Imininingwane Ejwayelekile: Lorem ipsum dolor sit
amet, consectetur adipiscing elit. Sed ukwenza i-eiusmod tempor incididunt
ut labore et dolore magna aliqua.
Imininingwane Yemvelo: Lorem ipsum
dolor sit amet, consectetur adipiscing elit. Sed do eiusmod tempor
incididunt ut labore et dolore magna aliqua.

Izitifiketi: Lorem ipsum dolor sit amet,
consectetur adipiscing elit. Sed do eiusmod tempor incididunt ut
labore et dolore magna aliqua.
Inombolo yomkhiqizo: XXX-XXXX-XXXX

Imiyalo yokusetshenziswa komkhiqizo:

1. Ukufakwa nokulinganiswa kwezinsimbi:

Landela izinyathelo ezichazwe kubhukwana le-pipette ne-gripper
ukufakwa.

2. Ukuthutha:

Ngomnyakazo omfushane, bheka isigaba 2.5 semanuwali. Ngoba
ukuhamba ibanga elide, landela imihlahlandlela enikeziwe. Okuhambayo okujwayelekile
iseluleko siyatholakala.

3. Izixhumanisi:

Qinisekisa ukuxhunywa kwamandla ngendlela efanele njengoba kuchaziwe kumanuwali.
Xhuma i-USB namadivayisi asizayo njengoba kudingeka. Izixhumanisi zenethiwekhi
kufanele isungulwe ngokulandela imiyalelo.

4. Umklami Wephrothokholi:

Qonda izimfuneko Zomklami Wephrothokholi futhi ufunde ukuthi kanjani
ukudizayina amaphrothokholi amasha noma uguqule ezikhona ngokwelebhu yakho
izidingo.

5. I-Python Protocol API:

Hlola ukubhala nokusebenzisa imibhalo usebenzisa i-Python Protocol API.
Zitholele izici ezikhethekile zePython ukuze uthole ukusebenza okuthuthukisiwe.

6. Amaphrothokholi we-OT-2:

Funda mayelana ne-OT-2 Python protocol, OT-2 JSON protocol, kanye
Amaphrothokholi emojula kazibuthe ezinhlobo ezahlukene zokuhlolwa.

Imibuzo Evame Ukubuzwa (FAQ):

Q: Ngiyixazulula kanjani inkinga uma irobhothi lingahambi njenge
kulindeleke?

A: Hlola uxhumano lwamandla, qinisekisa ukulinganiswa okufanele kwe
amathuluzi, futhi uqinisekise ukuthi azikho izithiyo ku
indlela yerobhothi.

Q: Ngingasebenzisa ama-pipettes angokwezifiso nge-Opentrons Flex?

A: I-Opentrons incoma ukusebenzisa ama-pipettes ahambisanayo ngokulungile
ukusebenza nokunemba.

“`

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I-Opentrons Flex
Incwadi Yeziqondiso
Inkampani Opentrons Labworks Inc.
Disemba 2023

© OPENTRONS 2023 Opentrons FlexTM (Opentrons Labworks, Inc.) Amagama abhalisiwe, izimpawu zokuthengisa, njll. ezisetshenziswe kulo mbhalo, ngisho noma zingamakwanga kanjalo ngokuqondile, akufanele zithathwe njengezingavikelwe umthetho.

Okuqukethwe
Isandulela . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 Isakhiwo sale manuwali . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Amanothi nezixwayiso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Isahluko 1: Isingeniso. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 1.1 Siyakwamukela ku-Opentrons Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Yini entsha ku-Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Iziteshi zokusebenzela ze-Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 1.2 Ulwazi lokuphepha . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Izimpawu zokuphepha. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Izixwayiso zokuphepha ngogesi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Ezinye izexwayiso zokuphepha . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 Izexwayiso zokuphepha . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 Ukuphepha kwezinto eziphilayo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Intuthu enobuthi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Uketshezi oluvuthayo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 1.3 Ukuthobela imithetho . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Ukuphepha . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Ukuhambisana kwe-electromagnetic . . . . . . . . . . . . . . . . . . . . . . . . .20 izexwayiso namanothi e-FCC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Ukuthobela i-ISED yaseCanada . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Isixwayiso semvelo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Ukuqinisekiswa kwe-Wi-Fi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Isahluko 2: Ukufakwa Nokuthutha . . . . . . . . . . . . . . . . . . . . . . . 22 2.1 Izidingo zokuphepha nokusebenza. . . . . . . . . . . . . . . . . . . . .22 Ibekwa kuphi i-Opentrons Flex . . . . . . . . . . . . . . . . . . . . . . . .22 Ukusetshenziswa kwamandla . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 Izimo zemvelo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 2.2 Unboxing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Umzamo nesikhathi esidingekayo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Ikhreyithi nezinto zokupakisha . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Izakhi zomkhiqizo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 Ingxenye 1: Khipha ikhreyithi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27 Ingxenye 2: Khipha i-Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29 Ingxenye 3: Ukuhlanganiswa kokugcina namandla kuvuliwe. . . . . . . . . . . . . . . . . . .32

2.3 Ukugijima kokuqala . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Vula amandla. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Xhuma kunethiwekhi noma kwikhompyutha . . . . . . . . . . . . . . . . . . . . .36 Faka izibuyekezo zesofthiwe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37 Namathisela I-Emergency Stop Pendant . . . . . . . . . . . . . . . . . . . . . . 38 Nikeza irobhothi lakho igama . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
2.4 Ukufakwa nokulinganiswa kwezinsimbi . . . . . . . . . . . . . . . . . 38 Ukufakwa kwe-Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 Ukufakwa kwe-Gripper. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
2.5 Ukuthutha . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 Ukunyakaza okufushane. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Ukuhamba amabanga amade . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Iseluleko esivamile esishukumisayo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 Imicabango yokugcina mayelana nokunyakaza. . . . . . . . . . . . . . . . . . . . . . . . . . .44
Isahluko 3: Incazelo Yesistimu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 3.1 Izingxenye zomzimba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45 Uhlaka nendawo ebiyelwe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 Idekhi nendawo yokusebenza. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 Stagindawo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47 Ukulungiswa kwedekhi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 Ibhodlela likadoti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49 Stagizindawo Slots . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49 Uhlelo lokunyakaza . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 Isikrini esithintwayo nezibonisi ze-LED . . . . . . . . . . . . . . . . . . . . . . . . .51 3.2 Amapayipi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 Pipette Imininingwane. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Ukulinganisa kwe-Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 I-adaptha yokubeka ithiphu ye-Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Ukuthatha ithiphu ingxenye. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 Izinzwa ze-Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 Izibuyekezo ze-firmware ze-Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 3.3 Igripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 Imininingwane ye-Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Ukulinganisa kwe-Gripper. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Ukubuyekezwa kwe-firmware ye-Gripper. . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 3.4 I-Emergency Stop Pendant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 Isetshenziswa nini i-E-stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 Ukuzibandakanya nokukhulula i-E-stop . . . . . . . . . . . . . . . . . . . . .60

3.5 Izixhumanisi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 Ukuxhumeka kwamandla . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 USB nokuxhumana okuyisiz. . . . . . . . . . . . . . . . . . . . . . . . .62 Izixhumanisi zenethiwekhi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
3.6 Imininingwane yesistimu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63 Ukucaciswa okujwayelekile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63 Ukucaciswa kwemvelo . . . . . . . . . . . . . . . . . . . . . . . . . .64 Izitifiketi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 Inombolo yeserial . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64
Isahluko 4: Amamojula. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 4.1 Amamojula asekelwe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66 4.2 Isistimu ye-module caddy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 4.3 Ukulinganiswa kwemojuli . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68 Ungalinganisa nini amamojula. . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 Ungalinganisa kanjani amamojula . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 4.4 Imojuli ye-Heater-Shaker GEN1 . . . . . . . . . . . . . . . . . . . . . . . . . .70 Izici ze-Heater-Shaker. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70 Ukucaciswa kwe-Heater-Shaker . . . . . . . . . . . . . . . . . . . . . . . . . .72 4.5 I-Magnetic Block GEN1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 Izici ze-Magnetic Block. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 Ukucaciswa kwe-Magnetic Block. . . . . . . . . . . . . . . . . . . . . . . . .74 4.6 I-Temperature Module GEN2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .75 Izici Zemojuli Yezinga Lokushisa . . . . . . . . . . . . . . . . . . . . . . . . .75 Izicaciso Zemojuli Yezinga Lokushisa . . . . . . . . . . . . . . . . . . . .77 4.7 Thermocycler Module GEN2. . . . . . . . . . . . . . . . . . . . . . . . . . . .77 Izici ze-Thermocycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .78 Ukucaciswa kweThermocycler . . . . . . . . . . . . . . . . . . . . . . . . . . .79
Isahluko 5: I-Labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 5.1 Imiqondo yelebhu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Uhlelo lokusebenza njengehadiwe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Ilebhu njengedatha . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Ilebhu yangokwezifiso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81 5.2 Amadamu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Amadamu anomthombo owodwa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Amadamu anemithombo eminingi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Amadamu nezincazelo ze-API . . . . . . . . . . . . . . . . . . . . . . . . 83 Ilebhu yereservoir yangokwezifiso. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 5.3 Izingcwecwe zemithombo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

Amapuleti angu-6. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 12-imithombo amapuleti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 24-imithombo amapuleti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 48-imithombo yamapuleti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 96-imithombo amapuleti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 384-imithombo amapuleti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 Ama-adaptha amapuleti omthombo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 Amapuleti emithombo kanye nezincazelo ze-API. . . . . . . . . . . . . . . . . . . . . . . . .87 Ilebhu yepuleti lemithombo yangokwezifiso. . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 5.4 Amathiphu namathiphu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 Ama-racks amathiphu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 Ukuhambisana kwe-Tippipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 I-adaptha yokubeka ithiphu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 5.5 Amashubhu nama-tube rack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90 Izinhlanganisela zamashubhu namarack . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 6-amapayipi amashubhu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 10-amashubhu racks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 15-amapayipi amashubhu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 24-amapayipi amashubhu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Izincazelo ze-API ye-Tube rack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Ilebhu yerack yeshubhu yangokwezifiso . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 5.6 Amabhulokhi e-Aluminium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 Ipuleti eliphansi eliyisicaba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 24-ibhulokhi ye-aluminium yomthombo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 96-ibhulokhi ye-aluminium yomthombo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Ama-adaptha azimele . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Inhlanganisela yelebhu yebhulokhi ye-Aluminium . . . . . . . . . . . . . . . .94 24-inhlanganisela ye-aluminium block labware yemithombo engu-XNUMX . . . . . . . . .95 96-inhlanganisela ye-aluminium block labware yemithombo engu-XNUMX . . . . . . . . .95 5.7 I-Labware kanye ne-Opentrons Flex Gripper . . . . . . . . . . . . . . .96 5.8 Izincazelo zelebhu yangokwezifiso . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 Ukudala izincazelo zelebhu yangokwezifiso . . . . . . . . . . . . . . . . . . .97 I-schema yelebhu ye-JSON . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99 Izincazelo zelebhu ye-JSON . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104 Isahluko 6: Ukuthuthukiswa Kwezinqubo . . . . . . . . . . . . . . . . . . . . . . . . . . .105 6.1 Amaphrothokholi enziwe ngaphambilini . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105 Ilabhulali Yephrothokholi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Isevisi ye-Custom Protocol Development 105 . . . . . . . . . . . . . . . .

6.2 Umklami Wephrothokholi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109 Izidingo Zomklami Wephrothokholi . . . . . . . . . . . . . . . . . . . . . . 109 Ukuklama iphrothokholi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Ukulungisa amaphrothokholi akhona . . . . . . . . . . . . . . . . . . . . . . . . . . 114
6.3 I-Python Protocol API . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 Ukubhala nokusebenzisa izikripthi . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 izici ezikhethekile zePython . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
6.4 OT-2 amaphrothokholi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117 OT-2 Python protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118 OT-2 JSON amaphrothokholi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120 Amaphrothokholi emojula kaMagnetic. . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Isahluko 7: Isoftware nokusebenza. . . . . . . . . . . . . . . . . . . . . . . . . . 121 7.1 Ukusebenza kwesikrini esithintwayo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 Ideshibhodi yerobhothi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 Ukuphathwa kwephrothokholi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122 Imininingwane yephrothokholi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124 Qalisa ukusetha. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I-127 Labware Position Check. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128 Qalisa inqubekelaphambili. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130 Ukuqeda ukugijima . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131 Ukuphathwa kwezinsimbi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132 Izilungiselelo zerobhothi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133 Ukumiswa kwedekhi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136 7.2 Uhlelo lokusebenza lwe-Opentrons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 Ukufakwa kohlelo lokusebenza . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 Ukudlulisa amaphrothokholi ku-Flex. . . . . . . . . . . . . . . . . . . . . . . . . 140 Isimo nezilawuli zemojuli. . . . . . . . . . . . . . . . . . . . . . . . . . . 142 Iphrothokholi yakamuva iyasebenza . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143 7.3 Ukusebenza okuthuthukile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144 Jupyter Notebook. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144 Ukusebenza komugqa womyalo phezu kwe-SSH. . . . . . . . . . . . . . . . . . . . 145
Isahluko 8: Isondlo Nesevisi. . . . . . . . . . . . . . . . . . . . . . . .147 8.1 Ukuhlanza iFlex yakho . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Ngaphambi kokuthi uqale . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Lokho ongakuhlanza . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Izixazululo zokuhlanza. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148 Ukuhlanza uhlaka nephaneli yamafasitela. . . . . . . . . . . . . . . . . . . . 148 Ukuhlanza amadekhi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148

Ukuhlanza i-gantry. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 Ukuhlanza imfucuza. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 8.2 Ukuhlanza amapayipi namathiphu. . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 Ukukhishwa kwe-Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Amathiphu angu-150 okuhlanza i-pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151 8.3 Ukuhlanza into yokubamba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152 8.4 Amamojula okuhlanza. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 Ukuhlanzwa kwamamojula okujwayelekile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 Thermocycler seals. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 8.5 Ilebhu ephephile ye-Autoclave . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 8.6 I-Service Flex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 izinsiza ze-Opentrons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 Ukufaneleka kokufakwa nokusebenza. . . 155 Ukugcinwa kokuvimbela . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156 Iwaranti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157 Isithasiselo A: Uhlu lwamagama . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .158 Isithasiselo B: Imibhalo Eyengeziwe . . . . . . . . . . . . . . . . . . . . . .170 B.1 I-Opentrons Knowledge Hub . . . . . . . . . . . . . . . . . . . . . . . . . . . . 170 B.2 Amadokhumenti e-Python Protocol API. . . . . . . . . . . . . . . . . . . 170 B.3 Ireferensi ye-Opentrons HTTP API. . . . . . . . . . . . . . . . . . . . . . . . 171 B.4 Amadokhumenti kanjiniyela . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171 Isithasiselo C: Isofthiwe Yomthombo Ovulekile . . . . . . . . . . . . . . . . . . . . . . . . .172 C.1 Ama-Opentrons ku-GitHub . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172 C.2 Opentrons monorepo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173 C.3 Ezinye izinqolobane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175 Isithasiselo D: Ukusekela kanye Nemininingwane Yokuxhumana . . . . . . . . . . . . . . .176 D.1 Ukuthengisa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 D.2 Ukusekela . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 D.3 Ulwazi lwebhizinisi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177

Isandulelo
Siyakwamukela kubhukwana lemiyalo yerobhothi elibamba uketshezi le-Opentrons Flex. Leli bhukwana likuqondisa cishe kukho konke odinga ukukwazi ukuze umise futhi usebenzise i-Flex, ugxile ezihlokweni ezifanele kakhulu kubasebenzisi bansuku zonke be-Flex endaweni yaselabhu.
Isakhiwo sale manuwali
I-Opentrons Flex iwuhlelo oluyinkimbinkimbi, ngakho-ke ziningi izindlela ezihlukene zokufunda yonke into engayenza. Zizwe ukhululekile ukugxumela ngqo esahlukweni esikhuluma nganoma yisiphi isihloko ofuna ukwazi ngaso! OkwesiboneloampNokho, uma usunayo i-Flex esethiwe elebhu yakho, ungakwazi ukweqa udlule isahluko Ukufaka Nokuthuthwa.
Uma ukhetha indlela eqondisiwe, leli bhukwana lakhiwe ngendlela yokuthi ungakwazi ukulilandela kusukela ekuqaleni uye ekugcineni.
Funda ngeFlex. Izici ezihlukanisayo ze-Flex zibalwe eSahlukweni 1: Isingeniso. Isethulo siphinde sihlanganise nolwazi olubalulekile lokuphepha nolwazi lokulawula.
Qala nge-Flex. Uma udinga ukumisa i-Flex yakho, landela imiyalelo enemininingwane eseSahlukweni sesi-2: Ukufakwa Nokuthuthwa. Bese uzijwayeza izingxenye ze-Flex eSahlukweni 3: Incazelo Yesistimu.
Setha idekhi yakho. Ukulungisa idekhi kunika amandla izinhlelo zokusebenza zesayensi ezahlukahlukene ku-Flex. Isahluko 4: Amamojula achaza amapherili e-Opentrons ongawafaka kuwo noma ngaphezulu kwedekhi ukuze wenze imisebenzi ethile yesayensi. Isahluko 5: I-Labware ichaza ukuthi kusetshenzwa kanjani ngemishini yokubamba uketshezi.
Qalisa iphrothokholi. Ukusetshenziswa okuyinhloko kwe-Flex kusebenzisa izinqubo zesayensi ezimisiwe, ezaziwa ngokuthi amaphrothokholi. Isahluko 6: Ukuthuthukiswa Kwephrothokholi kunikeza izindlela ezimbalwa zokuthola amaphrothokholi esenziwe ngomumo noma ukuziklama ngokwakho. Ukuze usebenzise umthetho olandelwayo, landela imiyalelo eseSahlukweni 7: Isofthiwe nokusebenza, esineziyalezo zokwenza eminye imisebenzi nokwenza izilungiselelo zerobhothi ngokwezifiso zakho.
Gcina i-Flex isebenza. Landela iseluleko esiseSahlukweni 8: Ukunakekela Nesevisi ukuze ugcine i-Flex yakho ihlanzekile futhi isebenza kahle. Noma bhalisela enye yezinkonzo ze-Opentrons ezisohlwini lapho futhi usivumele sikunakekelele i-Flex.
Funda okwengeziwe. Usadinga okunye? Bheka izithasiselo. Isithasiselo A: Uhlu lwamagama luchaza amagama ahlobene ne-Flex. Isithasiselo B: Imibhalo Eyengeziwe ikukhombisa izinsiza ezengeziwe zemikhiqizo ye-Opentrons kanye nekhodi yokubhala ukuze ulawule i-Flex.

I-OPENTRONS FLEX

ISANDULELA
I-Appendix C: I-Open-Source Software ichaza ukuthi isofthiwe ye-Opentrons isingathwa kanjani ku-GitHub njengesisetshenziswa sabo bobabili abathuthukisi nabangewona onjiniyela.
Isithasiselo D: Ulwazi Lokusekela Nokuxhumana lubala ukuthi ungathintana kanjani ne-Opentrons uma udinga usizo olungaphezu kwalokho okuhlinzekwa yimibhalo yethu.
Amanothi nezixwayiso
Kuyo yonke le manuwali, uzothola inothi efomethwe ngokukhethekile namabhulokhi wesexwayiso. Amanothi anikeza ulwazi oluwusizo olungase lungabonakali ngesikhathi esivamile sokusebenzisa i-Flex. Naka ngokukhethekile izexwayiso-zisetshenziswa kuphela ezimeni lapho usengozini yokulimala komuntu siqu, ukulinyazwa kwezinto zokusebenza, ukulahlekelwa noma ukumoshwa kwe-s.ampama-les noma ama-reagents, ukulahleka kwedatha, noma okunye ukulimala. Amanothi nezixwayiso zibukeka kanjena:
Sample Qaphela: Lena into okufanele uyazi, kodwa ayenzi ingozi.
Sample Isexwayiso: Lena into okumele uyazi ngoba kunengozi ehambisana nakho.

I-OPENTRONS FLEX

ISAHLUKO 1
Isingeniso

Lesi sahluko sikwethulela i-Opentrons Flex ecosystem, okuhlanganisa ukwakheka kwesistimu iyonke kanye nezilungiselelo ezitholakalayo zendawo yokusebenza. Kubandakanya futhi imininingwane ebalulekile yokuthobela kanye nokuphepha, okufanele uphinde uyenzeview ngaphambi kokumisa irobhothi lakho le-Opentrons Flex. Ukuze uthole imininingwane eyengeziwe ngezici ze-Opentrons Flex, bona Incazelo Yesistimu isahluko.

1.1 Siyakwamukela ku-Opentrons Flex
I-Opentrons Flex iyirobhothi elibamba uketshezi elakhelwe ukukhiqiza okuphezulu nokugeleza komsebenzi okuyinkimbinkimbi. Irobhothi le-Flex liyisisekelo sesistimu ye-modular ehlanganisa ama-pipettes, i-labware gripper, ukulungiswa kwedekhi, amamojula akudekhi, kanye ne-labware - konke ongakwazi ukuzishintshanisa ngokwakho. I-Flex yakhelwe ngesikrini esithintwayo ukuze usebenze ngaso ngqo ebhentshini ngelebhu, noma ungayilawula usuka kuyo yonke ilebhu yakho ngohlelo lokusebenza lwe-Opentrons noma ama-API ethu omthombo ovulekile.
Iziteshi zokusebenzela ze-Flex ziza nazo zonke izinto zokusebenza - irobhothi, i-Hardware, ne-labware - oyidingayo ukuze uqalise ngokuzenzakalelayo imisebenzi evamile yalebhu. Kwezinye izinhlelo zokusebenza, i-Opentrons Flex isebenzisa isofthiwe yomthombo ovulekile ngokugcwele kanye ne-firmware, futhi iyi-reagent- kanye ne-labware-agnostic, ekunikeza ukulawula ukuthi uklama futhi uqhuba kanjani izivumelwano zakho.

Yini entsha ku-Flex
I-Opentrons Flex iyingxenye yochungechunge lwesibambi se-Opentrons samarobhothi. Abasebenzisi be-Opentrons Flex bangase bajwayelene ne-Opentrons OT-2, irobhothi lethu lomuntu siqu lamapayipi. I-Flex idlulela ngalé kwamakhono e-OT-2 ezindaweni ezimbalwa ezibalulekile, iletha ukudlula okuphezulu nesikhathi sokuhamba.

Faka i-Pipette throughput
Amakhono we-Pipette namathiphu

Incazelo
Amapayipi e-Flex aneziteshi ezingu-1, 8, noma ezingu-96. I-pipette ye-96-channel isebenza emithonjeni eminingi izikhathi ezingu-12 ngesikhathi esisodwa kune-OT-2 pipette enkulu kunazo zonke.
Ama-Flex pipette anebanga levolumu elikhudlwana (1 µL, 50 µL) futhi angasebenza nganoma iyiphi ivolumu yamathiphu e-Opentrons Flex. Lokhu ukuthuthuka okungaphezu kwamapayipi e-OT-5, anobubanzi obuncane futhi okufanele asebenzise amathiphu anebanga levolumu elifanayo.

I-OPENTRONS FLEX

ISAHLUKO 1: ISINGENISO

I-Gripper Automated Calibration I-Touchscreen Module caddies I-Deck slot ixhumanisa udoti ohambisekayo Usayizi nesisindo

I-Opentrons Flex Gripper icosha bese ihambisa ilebula edeskini ngokuzenzakalelayo, ngaphandle kokungenelela komsebenzisi. I-gripper inika amandla ukuhamba komsebenzi okuyinkimbinkimbi ngaphakathi kokusebenza kwephrothokholi eyodwa.
Ukulinganiswa kokuma kwe-Flex pipettes kanye ne-gripper ku-othomathikhi ngokugcwele. Cindezela inkinobho eyodwa, futhi ithuluzi lizoya kumaphoyinti enziwe ngokunemba kudekhi ukuze kunqunywe indawo ekuyo ngqo, ilondoloza leyo datha ukuze isetshenziswe kumaphrothokholi akho.
I-Flex inesixhumi esibonakalayo sesikrini esithintwayo esikuvumela ukuthi usilawule ngokuqondile, ngaphezu kokusebenzisa i-Opentrons App. Sebenzisa isikrini esithintwayo ukuze uqale ukusebenza kwephrothokholi, hlola isimo somsebenzi, futhi ushintshe izilungiselelo khona kanye irobhothi.
Amamojula we-Flex angena kuma-caddies athatha isikhala ngaphansi kwedekhi. Ama-Caddies abeka ilebhu yakho eduze nendawo yedekhi futhi avumele umzila wekhebula ongaphansi kwedekhi. Ama-Caddies anika amandla ukulungiselelwa okwengeziwe kwemojula nelebhu edekhini.
Izikhala zedekhi ku-Flex zinombolwe ngohlelo lokudidiyela (A1D4) olufana nendlela imithombo ebalwa ngayo kwilebhu.
Umgqomo kadoti ungaya ezindaweni eziningi zedekhi ku-Flex. Indawo ezenzakalelayo (slot A3) yindawo enconyiwe. Ungasebenzisa futhi i-gripper ukuze ulahle udoti ku-chute kadoti ongayikhetha.
I-Flex inkulu kancane futhi isinda kakhulu kune-OT-2. Imisebenzi yokufaka ku-Flex idinga usizo lukazakwethu zelebhu.

Ukuqhathanisa okuningiliziwe kokucaciswa kobuchwepheshe berobhothi kuyatholakala kuma-Opentrons webindawo.
Womabili amarobhothi e-Flex ne-OT-2 asebenzisa isofthiwe yethu yomthombo ovulekile, futhi i-Opentrons App ingalawula zombili izinhlobo zamarobhothi ngesikhathi esisodwa. Nakuba izimiso ze-OT-2 zingakwazi ukusetshenziswa ngokuqondile ku-Flex, kuqondile ukuzivumelanisa nezimo (bona isigaba se-OT-2 Protocols sesahluko Sokuthuthukiswa Kwephrothokholi ukuze uthole imininingwane).

Flex workstations
Iziteshi zokusebenzela ze-Opentrons Flex zifaka irobhothi le-Flex, izesekeli, ama-pipettes kanye ne-gripper, amamojula asedekhini, kanye nelebhu edingekayo ukuze kuzenzakalelayo uhlelo oluthile. Zonke izingxenye zendawo yokusebenza ziyi-modular. Uma udinga ukushintsha izinhlelo zokusebenza, ungakwazi ukwengeza noma ukushintshanisa enye ihadiwe ye-Flex nezinto ezisebenzisekayo ezihambisanayo.

I-OPENTRONS FLEX

ISAHLUKO 1: ISINGENISO
INDAWO YOKUSEBENZA YE-NGS
I-Opentrons Flex NGS Workstation yenza ngokuzenzakalelayo ukulungiselelwa komtapo wezincwadi we-NGS. Ingakwazi ukwenza ngokuzenzakalelayo ukuhamba komsebenzi okulandelanayo isebenzisa noma iyiphi isistimu ehamba phambili ye-reagent, okuhlanganisa ukuhlukana- kanye tagukulungiselelwa komtapo wolwazi okusekelwe ekukhulumeni.
Ngaphezu kwerobhothi le-Flex, i-NGS Workstation ihlanganisa:
I-Gripper Ukukhetha kokucushwa kwe-pipette
Amapayipi amabili we-8-Channel (1 µL kanye no-50 µL) 5-Channel Pipette (1000 µL) Waste Chute Magnetic Block Temperature Module Thermocycler Module Labware kit enamathiphu okuhlunga, amashubhu e-microcentrifuge, ama-reservoir, namapuleti e-PCR
I-PCR WORKSTATION
I-Opentrons Flex PCR Workstation yenza ngokuzenzakalelayo ukusethwa kwe-PCR kanye nokugeleza komsebenzi we-thermocycling kuze kufike kumasekhondi angama-96.ampLes. Kungaba aliquot ama-reagents abandayo kanye ne-sampingena epuleti le-PCR elinemithombo engu-96. Ngokungezwa kwe-Automated Thermocycler Module, sebenzisa i-gripper ukuze ulayishe ipuleti ku-Thermocycler, bese usebenzisa uhlelo lwakho olukhethile lwe-PCR.
Ngaphezu kwerobhothi le-Flex, i-PCR Workstation ihlanganisa:
I-Gripper Ukukhetha kokucushwa kwe-pipette
I-1-Channel Pipette (1 µL) kanye ne-50-Channel Pipette (8 µL) 1-Channel Pipette (50 µL) Ikhithi ye-Waste Chute Temperature Module Labware Labware enamathiphu okuhlunga, amashubhu e-microcentrifuge, ama-reservoir, namapuleti e-PCR

I-OPENTRONS FLEX

ISAHLUKO 1: ISINGENISO
I-NUCLEIC Acid UMSEBENZI WOKUSEBENZA
I-Opentrons Flex Nucleic Acid Extraction Workstation yenza ngokuzenzakalelayo ukuhlukaniswa nokuhlanza kwe-DNA/RNA. Isebenzisa i-Magnetic Block ukuhlukanisa ubuhlalu kazibuthe, kanye ne-Heater-Shakerample lysis kanye nokumiswa kabusha kobuhlalu kazibuthe.
Ngaphezu kwerobhothi le-Flex, i-Nucleic Acid Extraction Workstation ihlanganisa:
I-Gripper Ukukhetha kokucushwa kwe-pipette
I-1-Channel Pipette (5 µL) kanye ne-1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL) I-Waste Chute Magnetic Block Heater-Shaker Module Labware kit enamathiphu okuhlunga, izigcinalwazi, amapuleti emithombo ejulile ye-PCR,
MAGNETIC BEAD PROTEIN PURIFICATION WORKSTATION
I-Opentrons Flex Magnetic Bead Protein Purification Workstation yenza ngokuzenzakalelayo ukuhlanzwa kwamaprotheni amancane kanye nama-proteomicsampngilungiselela ukufika kuma-96 sampLes. Ihambisana nama-reagents amaningi asekelwe ku-magnetic-bead.
Ngaphezu kwerobhothi le-Flex, i-Protein Purification Workstation ihlanganisa:
I-Gripper Ukukhetha kokucushwa kwe-pipette
I-1-Channel Pipette (5 µL) kanye ne-1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL) I-Waste Chute Magnetic Block Heater-Shaker Module Labware kit enamathiphu okuhlunga, izigcinalwazi, amapuleti emithombo ejulile ye-PCR,
I-FLEX PREP WORKSTATION
I-Opentrons Flex Prep Workstation yenza ngokuzenzakalelayo ukuhamba komsebenzi okulula kwamapayipi. Lungiselela indawo yokusebenza nge-1-Channel kanye ne-8-Channel Pipettes ukuze wenze imisebenzi efana ne-sample transfer, sample ukuphindaphinda, futhi

I-OPENTRONS FLEX

ISAHLUKO 1: ISINGENISO
i-reagent aliquoting. Lungiselela indawo yokusebenza nge-96-Channel Pipette ukuze wenze i-high-throughput reagent aliquoting ne-plate stamping.
Ngaphezu kwerobhothi leFlex, iFlex Prep Workstation ihlanganisa:
Ukukhethwa kokucushwa kwe-pipette: 1-Channel Pipette (5 µL) kanye ne-1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL)
Ikhithi yelebhu enamathiphu okuhlunga, amashubhu e-microcentrifuge, nama-reservoirs
I-PLASMID PREP WORKSTATION
I-Opentrons Flex Plasmid Prep Workstation yenza ngokuzenzakalelayo ukukhishwa kwe-plasmid okususelwe ebuhlalu kazibuthe kanye nokugeleza komsebenzi wokuhlanza. Lesi siteshi sifakwe amapayipi evolumu ephezulu, i-Heater-Shaker Module, kanye ne-Magnetic Block ukuze kufakwe iningi lamakhemikhali asekelwe ebuhlalu.
Ngaphezu kwerobhothi le-Flex, i-Plasmid Prep Workstation ihlanganisa:
I-Gripper 1-Channel Pipette (5 µL) kanye ne-1000-Channel Pipette (8 µL) Ikhithi ye-Labware ye-Waste Chute Magnetic Block Heater-Shaker Module Labware enamathiphu okuhlunga, amashubhu e-microcentrifuge, ama-reservoir, amapuleti e-PCR, namapuleti emithombo ejulile
I-SYNBIO WORKSTATION
I-Opentrons Flex SynBio Workstation izenzela ngokuzenzakalelayo ukugeleza komsebenzi okuhlukahlukene kokwenziwa kwebhayoloji okufana nokuhlanganiswa kwe-DNA nokuhlanganisa. Isebenzisa i-Magnetic Block and Temperature Module ukuze isekele iningi lamakhemikhali asekelwe kubuhlalu. Engeza i-Thermocycler Module ukwenza incubations yesivalo esishisayo futhi ampama-liifications.
Ngaphezu kwerobhothi leFlex, iSynBio Workstation ihlanganisa:
I-Gripper 1-Channel Pipette (5 µL) kanye ne-1000-Channel Pipette (8 µL) I-Magnetic Block Temperature Module

I-OPENTRONS FLEX

ISAHLUKO 1: ISINGENISO

Ikhithi ye-Labware enamathiphu ajwayelekile nawokuhlunga, amashubhu e-microcentrifuge, ama-reservoirs, amapuleti e-PCR, namapuleti emithombo ejulile

1.2 Ulwazi lokuphepha
Irobhothi le-Opentrons Flex eliphatha uketshezi lenzelwe ukusebenza okuphephile. Bheka izicaciso nemihlahlandlela yokuthobelana kulesi sigaba ukuze uqinisekise ukusetshenziswa okuphephile kwe-Flex yakho. Le mihlahlandlela ihlanganisa ukusetshenziswa okuphephile kokufakwayo nokuxhunywayo komkhiqizo, okuhlanganisa amandla nokuxhumeka kwedatha, kanye namalebula ayisixwayiso atholakala erobhothini le-Flex kanye nezingxenyekazi zekhompyutha ezihlobene. Ukusebenzisa idivayisi ngendlela engeyona eshiwo kule manuwali kungase kubeke umsebenzisi nezinto zokusebenza engcupheni.

Izimpawu zokuphepha
Amalebula ahlukahlukene ku-Flex nakule manuwali akwexwayisa ngemithombo yokulimala noma ukulimala okungaba khona.

Uphawu

Incazelo
Isexwayiso: Ixwayisa abasebenzisi ngezimo ezingaba yingozi. Izenzo ezingase zibangele ukulimala komuntu siqu noma ukufa.
Isexwayiso: Ixwayisa abasebenzisi ngokulimala kwezisetshenziswa. Idatha elahlekile noma eyonakele. Ukuphazamiseka okungalungiseki komsebenzi owenziwayo.
Ukushaqeka kukagesi: Ihlonza izingxenye zensimbi ezingase zibe engcupheni yokushaqeka kagesi uma ithuluzi liphathwa ngendlela engafanele.

Indawo eshisayo: Ikhomba izingxenye zensimbi ezibeka engcupheni yokulimala komuntu ngenxa yokushisa okuphezulu/amazinga okushisa uma ithuluzi liphathwa ngendlela engafanele.

Iphuzu lokuncinza: Ikhomba izingxenye zensimbi ezingase zibe sengcupheni yokulimala komuntu siqu lapho zinyakaza.

I-OPENTRONS FLEX

ISAHLUKO 1: ISINGENISO

Uzothola amalebula alandelayo ku-Flex:
Amalebula empahla eqanjiwe Amalebula omthetho wokuthobela imithetho (isb., ETL) Amalebula ezingozi zikagesi Amalebula ajwayelekile asexwayiso Amalebula omkhiqizo Amalebula aphuzu Amaphuzu aphezulutage amalebula Izilinganiso Amandla amalebula

Izixwayiso zokuphepha zikagesi
Ngaso sonke isikhathi qaphela izexwayiso zokuphepha zikagesi ezilandelayo:

Uphawu

Incazelo
Xhuma irobhothi kusiyingi esimisiwe, Yekilasi 1. Bona isigaba Sokuxhuma Amandla kusahluko Sencazelo Yesistimu.

Ungaxhumeki (uxhume), nqamula (khipha ipulaki), noma usebenzise izintambo zikagesi ze-AC uma: Ikhebula liphukile noma lilimele. Ezinye izintambo, izintambo, noma izitsha ezinamathiselwe zibolile noma zonakele.
Ukusebenzisa izintambo zikagesi ezilimele kungabangela ingozi yokushaqeka kukagesi okuholela ekulimaleni okubi kakhulu noma ukulimala irobhothi.
Ungashintshi ikhebula lamandla e-AC ngaphandle uma uqondiswa yi-Opentrons Support.

Ukuze uthole ulwazi olwengeziwe mayelana nezidingo zikagesi, bheka isigaba sokusebenzisa amandla sesahluko sokufaka nokuthuthwa.

I-OPENTRONS FLEX

ISAHLUKO 1: ISINGENISO

Izixwayiso zokuphepha ezengeziwe
Ngaso sonke isikhathi qaphela izexwayiso ezilandelayo zokuphepha:

Uphawu

Incazelo
I-Opentrons Flex ayiqinisekisiwe ukuthi isetshenziswe noketshezi oluqhumayo noma oluvuthayo. Ungalayishi amapuleti, amashubhu, noma izitsha eziqukethe uketshezi oluqhumayo noma oluvuthayo erobhothini noma usebenzise ithuluzi elinoketshezi oluqhumayo noma oluvuthayo endaweni ebiyelwe.
Sebenzisa izinqubo ezinhle zaselabhorethri futhi ulandele izixwayiso zomkhiqizi lapho usebenza ngamakhemikhali. I-Opentrons ayinasibopho noma ibophezelekile nganoma yimuphi umonakalo ngenxa, noma ngenxa, ukusetshenziswa kwamakhemikhali ayingozi.

I-Flex inesisindo esingu-88.5 kg (195 lbs). Ngenxa yalokho, kudinga abantu ababili ukuyiphakamisa futhi bayihambise ngokuphepha. Bheka isigaba Sokuthutha esahlukweni Ukufaka Nokuthuthwa.

I-Flex kufanele ibekwe endaweni ekwazi ukusekela isisindo sayo esingu-88.5 kg (195 lbs) enendawo eyanele ukuze ifake irobhothi kanye nebanga eliphansi lokuvunyelwa (20 cm/8 in). Bheka isigaba Sezimfuneko Zokuphepha Nokusebenza kusahluko sokufaka nokuthuthwa.
I-Flex ingakhipha ukudlidliza ngenkathi isebenza. Beka irobhothi endaweni eqinile, ileveli, futhi engangeni manzi enamajoyinti ahlanganisayo noma ashiselwe. Bheka isigaba Sezimfuneko Zokuphepha Nokusebenza kusahluko sokufaka nokuthuthwa.

Izixwayiso zokuphepha
Ukusiza ukuvikela i-Flex emonakalweni, landela lezi zinyathelo zokuphepha:

I-OPENTRONS FLEX

ISAHLUKO 1: ISINGENISO

Uphawu

Incazelo
Sebenzisa i-labware ethobela i-ANSI/SLAS noma egunyazwe i-Opentrons. Bheka isahluko seLabware.
Gcina izinto ezonakalisayo, ama-ejenti, noma ezinye izinto ezilimazayo kude nerobhothi.

Ukuphepha kwebhayoloji
Phatha izibonelo nama-reagents aqukethe izinto ezithathwe kubantu njengama-agent angaba amagciwane. U-Opentrons uncoma ukusebenzisa izinqubo zaselabhorethri eziphephile njengoba kuchazwe ku-Biosafety in Microbiological and Biomedical Laboratories (BMBL) 6th Edition.
Ngaphansi kwezimo ezijwayelekile, i-Flex ayiwakhi ama-aerosols atholakalayo oketshezini oluwumthombo. Kodwa-ke, ngaphansi kwezimo ezithile, kungenzeka ukukhiqiza ama-aerosol kusuka oketshezini oluwumthombo. Uma usebenza nge-biosafety level 2 noma uketshezi lomthombo omkhulu, cabanga ukuthatha izinyathelo zokuphepha ngokumelene nokuchayeka kwe-aerosol, ngokuvumelana nezindikimba ezilawulayo zendawo yangakini. Ukuze unciphise ingozi engaba khona yokuchayeka i-aerosol erobhothini, qinisekisa ukuthi:
Yenza isondlo njengoba kuchazwe esahlukweni Sokulondolozwa Nesevisi. Faka kahle futhi uvikele wonke amakhava wezinsimbi, ama-pipette, amamojula, nelebhu. Sebenzisa indlela efanele yokufaka amapayipi ukuze usize ekunciphiseni ama-aerosol.
Intuthu enobuthi
Uma usebenza ngezincibilikisi eziguquguqukayo noma izinto ezinobuthi, sebenzisa isistimu yokungenisa umoya yaselabhorethri ukuze ukhiphe noma yimiphi imfucumfucu engase ikhiqizwe.
Uketshezi oluvuthayo
I-Flex ayizange ihlolwe ukuthi isetshenziswe noketshezi oluvuthayo futhi akufanele isetshenziswe noketshezi oluvuthayo.

I-OPENTRONS FLEX

ISAHLUKO 1: ISINGENISO

1.3 Ukuthobela imithetho
I-Opentrons Flex ithobela zonke izidingo ezisebenzayo zalawa mazinga alandelayo okuphepha kanye ne-electromagnetic.

Ukuphepha
I-ID yomthetho IEC/UL/CSA 61010-1 IEC/UL/CSA 61010-2-051

Isihloko
Izidingo zokuphepha kwezisetshenziswa zikagesi zokukala, zokulawula, kanye nokusetshenziswa kwelabhorethri Ingxenye 1: Izidingo Ezijwayelekile
Izidingo ezikhethekile zemishini yaselabhorethri yokuxutshwa nokunyakaziswa

Ukuhambisana kwe-electromagnetic

I-ID yomthetho EN/BSI 61326-1
I-FCC 47 CFR Ingxenye 15 Ingxenye Engaphansi B Ikilasi A IC ICES-003

Isihloko
Izisetshenziswa zikagesi zokukala, zokulawula, kanye nokusetshenziswa kwelabhorethri Izidingo ze-EMC Ingxenye 1: Izidingo Ezijwayelekile Ama-radiator angahlosiwe.
Ukuphathwa Kwe-Spectrum kanye Nokuphazamiseka KwezokuxhumanaOkubangela Izisetshenziswa Ezivamile Zobuchwepheshe Bolwazi (Kufaka I-Digital Apparatus)

Izexwayiso ze-FCC namanothi
Isexwayiso: Izinguquko noma ukuguqulwa kule yunithi okungagunyazwanga ngokucacile yi-Opentrons kungase kuqede igunya lomsebenzisi lokusebenzisa okokusebenza.
Le divayisi ithobela Ingxenye 15 yemithetho ye-FCC. Ukusebenza kungaphansi kokulandelayo:

I-OPENTRONS FLEX

ISAHLUKO 1: ISINGENISO
Le divayisi ingase ingabangeli ukuphazamiseka okuyingozi. Lolu cingo kufanele lwamukele noma yikuphi ukuphazamiseka okutholiwe, okuhlanganisa ukuphazamiseka okungase kubangele okungafunwa
ukusebenza.
Qaphela: Lesi sixhobo sihloliwe futhi kwatholakala ukuthi sithobela imikhawulo yedivayisi yedijithali Yekilasi A, ngokuvumelana neNgxenye 15 yemithetho ye-FCC. Le mikhawulo iklanyelwe ukuhlinzeka ngokuvikeleka okufanele ekuphazanyisweni okuyingozi lapho okokusebenza kusebenza endaweni yezohwebo. Lesi sixhobo sikhiqiza, sisebenzise, futhi singakhipha amandla efrikhwensi yomsakazo futhi, uma ingafakiwe futhi isetshenziswe ngokuhambisana nebhukwana lemiyalo, ingase ibangele ukuphazamiseka okuyingozi ezokuxhumana ngomsakazo. Ukusebenza kwalesi sisetshenziswa endaweni okuhlalwa kuyo kungase kubangele ukuphazamiseka okuyingozi lapho umsebenzisi kuyodingeka ukuthi alungise ukuphazamiseka ngezindleko zakhe.
Ukuhambisana ne-ISED yaseCanada
I-Canada ICES-003(A) / NMB-003(A)
Lo mkhiqizo uhlangabezana nezicaciso zobuchwepheshe ezisebenzayo ze-Innovation, Science and Economic Development Canada.
Le présent produit est conforme aux specifications techniques ezisebenzayo d'Innovation, Sciences et Developpement économique Canada.
Isixwayiso semvelo
Isexwayiso: Umdlavuza kanye Nokulimala Kwenzalo www.P65Warnings.ca.gov
Ukuqinisekiswa kwe-Wi-Fi
Imojula ye-Wi-Fi iqinisekisiwe ukuthi isetshenziswe ezifundeni eziningi:
I-United States (FCC): I-FCC Identifier UAY-W8997-M1216 European Economic Area (CE): Ayikho inkomba esesidlangalaleni (self-declaration) Canada (IC): Hardware Version Identification Number W8997-M1216 Japan (TELEC): Inombolo eqinisekisiwe 020-170034 I-India (WPC): Inombolo yokubhalisa ETA-SD-20191005525 (ukuzimemezela)

I-OPENTRONS FLEX

ISAHLUKO 2
Ukufakwa Nokuthuthwa
Lesi sahluko sichaza ukuthi ungayilungiselela kanjani ilebhu yakho i-Opentrons Flex, ukuthi ulisetha kanjani irobhothi, nokuthi ulihambisa kanjani uma kunesidingo. Ngaphambi kokuletha i-Flex yakho, qiniseka ukuthi ilebhu noma indawo yakho ihlangabezana nazo zonke izimfuneko ezisesigabeni Sezimfuneko Zokuphepha Nokusebenza. Uma sekuyisikhathi sokwenza i-Flex yakho isebenze, landela imiyalelo enemininingwane esesigabeni se-Unboxing, First Run, kanye nese-Instrument Installation and Calibration, noma sebenzisa isevisi ye-Opentrons Onsite Support Set Up. Futhi uma kwenzeka udinga ukuhambisa i-Flex yakho endaweni entsha, eduze noma kude, landela izinyathelo ezisesigabeni Sokuthuthwa.
2.1 Izidingo zokuphepha nokusebenza
Lapho ungabeka khona i-Opentrons Flex
I-Space siyimpahla ebalulekile cishe kuwo wonke amalebhu. I-Flex yakho izodinga okunye–kodwa hhayi okuningi kakhulu, njengoba yakhelwe ukuthi ilingane nengxenye yebhentshi lelebhu evamile. Qiniseka ukuthi unesikhala esihlangabezana nemibandela elandelayo.
Indawo yebhentshi: Imile, iqinile, ileveli, indawo engangeni manzi. Amathebula noma amabhentshi anamasondo (ngisho namasondo okukhiya) akunconywa. I-Flex ihamba ngokushesha futhi inesisindo esiningi, esinganyakazisa noma ukungalingani amatafula angasindi noma anyakazayo.
Ukuthwala isisindo: Irobhothi lilodwa linesisindo esingu-88.5 kg (195 lb) futhi kufanele liphakanyiswe kuphela abantu ababili abasebenza ndawonye. Beka irobhothi endaweni engakwazi ukusekela isisindo salo kalula kanye nesisindo sanoma yimaphi amamojula, ilebhu, uketshezi, noma ezinye izinto zelabhu ezizosetshenziswa ezinhlelweni zakho.
Indawo yokusebenza: Ubukhulu besisekelo serobhothi bungu-87 cm W x 69 cm D x 84 cm H (cishe 34″ x 27″ x 33″). I-Flex idinga u-20 cm (8″) wokususwa kwezinhlangothi nangemuva kumakhebuli, uxhumo lwe-USB, kanye nokukhipha umoya ophuma kumamojula ashisa futhi apholile.
Isexwayiso: Ungabeki izinhlangothi noma ngemuva kwe-Flex eguquguqukayo odongeni.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA

84 cm 33"

87 cm 34"
Ubukhulu besisekelo se-Opentrons Flex.

69 cm 27"

20 cm 8"

Phezulu view ye-Opentrons Flex, ekhombisa ubuncane bokuvunyelwa ohlangothini nangemuva.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA

Ukusetshenziswa kwamandla
I-Opentrons Flex kufanele ixhunywe endaweni ophuma kuyo odongeni endaweni noma eduze nendawo yebhentshi lapho uyifake khona. Xhuma i-Flex kuphela kumasekhethi angakwazi ukuthwala amandla ayo aphezulu:
Amandla okokufaka: 36 VDC, 6.1 A Ukusetshenziswa kokungenzi lutho: 30 W Ukusetshenziswa okujwayelekile: 40 W (ngesikhathi sokuqaliswa kwephrothokholi) Ukusetshenziswa okuphezulu: Cishe u-50 W
Ukusetshenziswa kwamandla okuqondile kuncike kulokhu:
Inani nohlobo lomnyakazo owenziwe phakathi kwephrothokholi. Isikhathi esichithwa irobhothi lingenzi lutho. Isimo sezibani erobhothini. Zingaki izinsimbi zomculo ezinamathiselwe.
Khumbula ukulandisa ngezinye izinto zikagesi ezidla amandla kusekethe efanayo, okuhlanganisa namamojula e-Flex anezinto zawo zamandla. OkwesiboneloampLe, i-Thermocycler Module inamandla okusetshenziswa kakhulu (630 W) amakhulu kakhulu kunerobhothi le-Flex ngokwalo. Uma kunesidingo, thintana nomphathi wendawo yakho ukuze uqiniseke ukuthi ihlangabezana nezidingo zamandla emishini yakho.

Izimo zemvelo
Izimo zemvelo zokusetshenziswa okunconyiwe, ukusetshenziswa okwamukelekayo, nokugcinwa kuyehluka:

Kunconyelwe ukusebenza kwesistimu

Kwamukelekile ekusebenzeni kwesistimu

Ukushisa kwe-ambient +20 kuya ku-+25 °C

+2 kuya +40 °C

Umswakama ohlobene I-Altitude

40%, ayincishisi
Cishe ku-500 m ngaphezu kwezinga lolwandle

30%, ayijiki (ngaphansi kuka-80 °C)
Kufika ku-2000 m ngaphezu kwezinga lolwandle

Isitoreji kanye nezinto zokuhamba
-10 kuya +60 °C
10%, ayijiki (ngaphansi kuka-85 °C)
Kufika ku-2000 m ngaphezu kwezinga lolwandle

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
I-Opentrons iqinisekise ukusebenza kwe-Opentrons Flex ezimeni ezinconyelwe ukusebenza kwesistimu, futhi ukusebenza kulezo zimo kufanele kunikeze imiphumela emihle. I-Flex iphephile ukusetshenziswa ezimweni ezamukelekayo ekusebenzeni kwesistimu, kodwa imiphumela ingase ihluke. Ungayikhanyisi noma usebenzise i-Flex ezimeni ezingaphandle kwaleyo mingcele. Izimo zokugcina nezokuthutha zisebenza kuphela lapho irobhothi linqanyuliwe ngokuphelele kumandla nezinye izinto zokusebenza.
2.2 Ukuvula ibhokisi
Siyakuhalalisela! I-Opentrons Flex yakho isifikile futhi usuyilungiselele isikhala elebhu yakho. Masivule lelo khreyithi lesilo, sisuse irobhothi, futhi sililungiselele ukusebenza. Ulwazi olukulesi sigaba luhlinzeka ngohlu lwezingxenye neziyalezo ezikuhambisa ezinyathelweni ezidingekayo ukuze i-Flex isuswe ebhokisini, imiswe, futhi ilungele ukusetshenziswa. Sihlukanise inqubo yokusetha ibe izingxenye ezintathu:
Ingxenye 1 ihlanganisa ukuqaqa ikhreyithi. Ingxenye 2 ihlanganisa ukuhoxisa i-Flex ekhrethini bese iyiyisa endaweni yokugcina yomhlangano. Ingxenye 3 ihlanganisa ukuhlanganiswa kokugcina kanye nokunika amandla irobhothi okokuqala ngqa.
Umzamo nesikhathi esidingekayo
Uzofuna ukucela uzakwethu welebhu ukuthi akusize ngenqubo yokukhipha ibhokisi, ukuphakamisa, ukuhambisa, kanye nokuhlanganisa. Uzodinga ukwenza ibhajethi cishe imizuzu engama-30 ukuya ehoreni kulo mzamo.
Qaphela: I-Flex idinga abantu ababili ukuyiphakamisa kahle. Futhi, ukuphakamisa nokuthwala iFlex ngezibambo zayo kuyindlela engcono kakhulu yokuhambisa irobhothi.
Ikhreyithi nezinto zokupakisha
Ukukhipha i-Flex kukunikeza irobhothi elimangalisayo, kodwa futhi usele namaphaneli amaningana amakhulu ekhreyithi kanye nezingxenye zokuthumela ezihlukene kanye nama-padding. Nakuba ungase ulahle lezi zinto, sikukhuthaza ukuthi ugcine lezi zinto uma indawo yokugcina ikhona. Iphakheji ingasetshenziswa kabusha, okusiza ukulungiselela i-Flex yakho ukuthi ithunyelwe uma kwenzeka udinga ukuyithumela kwenye indawo (isb., engqungqutheleni noma endaweni entsha) ngokuzayo.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
Izakhi zomkhiqizo
I-Flex ithumela izinto ezibalwe ngezansi. Ama-Pipettes, i-gripper, namamojula afika emaphaketheni ahlukene asuka ekhreyithi elikhulu le-Flex, ngisho noma uwathenge ndawonye njengendawo yokusebenza.

(1) Irobhothi le-Opentrons Flex

(1) ikhebula le-USB

(1) Ikhebula lamandla

(1) Ikhebula le-Ethernet

(5) Okhiye be-L (12 mm hex, 1.5 mm hex, 2.5 mm hex, 3 mm hex,
T10 Torx)

(1) I-Emergency Stop Pendant

(1) Indawo yedekhi eneziqeshana ze-labware

(4) Iziqeshana zelebhu eyisipele

(1) I-Pipette calibration probe

(4) Ukuthwala izibambo namakepisi

(1) Iphaneli yewindi eliphezulu

(4) Amaphaneli ewindi aseceleni

(1) 2.5 mm i-hex screwdriver

(1) 19 mm isikrufu

(16 + izipele) Izikulufu zewindi (M4x8 mm ikhanda eliyisicaba)
26

(10) Izikulufo zesikhala sedekhi (ikhanda lesokhethi le-M4x10 mm)

(12) Izikulufo zesiqeshana zedekhi (ikhanda lesokhethi le-M3x6 mm)

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
Ingxenye 1: Khipha ikhreyithi
I-Opentrons ithumela i-Flex yakho ngekhreyithi eliqinile le-plywood. Ikhreyithi lokuthumela lisebenzisa i-hook ne-latch clamps ukuze uvikele amaphaneli aphezulu, ohlangothini, naphansi ndawonye. Ukusebenzisa ama-latches, esikhundleni sezinzipho noma izikulufu, kusho ukuthi ngeke udinge i-crowbar (noma amandla amaningi) ukuze uqaqe ikhreyithi, futhi ungalihlanganisa kabusha ngokuhamba kwesikhathi, uma kudingeka.
Qaphela: Amaphethelo e-Crate angaba rough ngesikhathi sokuthunyelwa. Ungase ufune ukusebenzisa amagilavu omsebenzi ukuze uvikele izandla zakho ezingxenyeni zezinkuni.
Ukuze ukhulule ama-latches, phenya ithebhu ye-latch phezulu bese uyiphendulela kwesokunxele (ngokuphambene newashi). Lesi senzo sinyakazisa i-clamp ingalo iphume kubakaki bayo abahambisanayo. Ungabe usususa ingalo ye-latch kude nekhreyithi.
1 Vula amaleshi ayisishiyagalombili ubambe phezulu emaceleni.

2 Khipha iphaneli ephezulu ngemuva kokukhulula ama-latches.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKA NOKUTHUTHUMELA 3 Sika uvule isikhwama sokuthumela esiluhlaza okwesibhakabhaka, khipha lezi zinto ku-padding, futhi uzibeke eceleni:
Ikhithi Yomsebenzisi Amandla, i-Ethernet, nezintambo ze-USB I-Emergency Stop Pendant
4 Khipha ucezu olungaphezulu lwe-foam padding ukuze uveze amaphaneli ewindi. I-padding ivikela amaphaneli aseceleni nangaphezulu.
5 Khipha amaphaneli ewindi uwabeke eceleni. Uzozinamathisela kamuva.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
6 Vula amalashi ayi-16 asele ebambe amaphaneli aseceleni komunye nomunye kanye nesisekelo sekhreyithi. 7 Khipha amapaneli aseceleni uwabeke eceleni.
Ingxenye 2: Khipha i-Flex
Ngemva kokuqeda izinyathelo eNgxenyeni yoku-1, manje kufanele ubone irobhothi elisesikhwameni sokuzivikela futhi linamathiselwe ezintweni ezifakwa ensimbi ezolintshi. Isikhwama sifaka irobhothi futhi silivikele endaweni engaphandle. Abakaki bensimbi bavikela irobhothi phansi kwekhreyithi. Amafreyimu wokuthumela amabili asekela irobhothi, asabalalisa isisindo salo ngokulinganayo, futhi aligcine liqinile ukuze linganyakazi ngesikhathi sokuthunyelwa. Qhubeka nokukhipha i-Flex futhi uyisuse kusisekelo sekhreyithi.
8 Usebenzisa isikrufu esingu-19 mm esisuka Kukhithi Yomsebenzisi, vula amabakaki ukusuka phansi kwekhreyithi. Ungalahla amabakaki, noma uwagcine ukuze uwasebenzise esikhathini esizayo.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA KANYE NOKUTHUTHUMELA 9 Donsa noma ugoqe isikhwama somkhumbi uye phansi ukuze uveze irobhothi lonke.
10 Ngosizo oluvela kuzakwenu ngelebhu, bamba izibambiso kumafreyimu wokuthumela awolintshi nhlangothi zombili zesisekelo irobhothi, phakamisa i-Flex uyisuse kusisekelo sekhreyithi, bese uyibeka phansi. Londoloza noma ulahle isisekelo sekhreyithi nohlaka lokuthumela.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKA NOKUTHUTHA 11 Usebenzisa ukhiye we-12 mm hex L kusuka Kukhithi Yomsebenzisi, susa amabhawodi amane abambe amafreyimu okuthumela ukuze
i-Flex. Londoloza noma ulahle amafreyimu namabhawudi.
12 Susa izibambo ezine ze-aluminium Kusethi Yomsebenzisi. Khipha izibambo ezindaweni ezifanayo ezibambe amabhawodi wozimele wokuhambisa ongu-12 mm.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
13 Ngosizo oluvela kuphathina wakho ngelebhu, phakamisa iFlex ngezibambo zayo zokuyiphatha bese uyiyisa endaweni yokusetshenzelwa ukuze uyihlanganise okokugcina.

Ingxenye 3: Ukuhlanganiswa kokugcina namandla avuliwe
Ngemva kokuthuthela i-Flex endaweni yokusebenza yesikhashana, noma ikhaya layo laphakade, sekuyisikhathi sokubeka ukuthinta kwerobhothi lakho elisha.
14 Uma uhambise irobhothi endaweni yalo yokugcina, yokusebenza, susa izibambo bese ufaka okepisi bokuqedela. Amakepisi avala izibambo zokuvuleka kufreyimu futhi anikeze irobhothi ukubukeka okuhlanzekile. Buyisela izibambo Kukhithi Yomsebenzisi ukuze uthole isitoreji.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
15 Buyisa amaphaneli aphezulu nasemaceleni kugwebu lokupakisha owubeke eceleni ngemva kokukhipha i-crate top.
16 Faka amaphaneli ewindi ku-Flex ngokulandela imininingwane yokulebula efilimini yokuvikela yangaphambili. Bese ususa ifilimu yokuzivikela.
17 Usebenzisa izikulufu zefasitela ezibevelelwe kanye ne-screwdriver engu-2.5 mm kusuka Kukhithi Yomsebenzisi, namathisela amaphaneli ewindi ku-Flex. Qiniseka ukuthi izimbobo ezigobekile (ezimise okwe-V) kumaphaneli ewindi zibheke ngaphandle (kuwe). Lokhu kuvumela izikulufu ukuthi zilingane nobuso bewindi.

Isexwayiso: Ukungaqondise kahle amaphaneli kungaholela ekulimaleni. I-screw torque eyeqile ingaqhekeza amaphaneli. Qinisa izikulufu ngesandla kuze kube yilapho amaphaneli ewindi evikelekile ngokunengqondo. Lokhu akusona isilingo samandla.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKA NOKUTHUTHUMELA 18 Usebenzisa i-screwdriver engu-2.5 mm Kukhithi Yomsebenzisi, susa izikulufu zokukhiya ku-gantry. Lezi
izikulufu zivimbela i-gantry ukuthi inganyakazi ngenkathi isendleleni. Izikulufu zokukhiya i-gantry zitholakala: Kujantshi ohlangothini lwesobunxele eduze nangaphambili kwerobhothi. Ngaphansi kwengalo ye-gantry eqondile. Kujantshi ongakwesokudla eduze nangaphambili kwerobhothi kubakaki abawolintshi. Kunezikulufu ezimbili lapha.
I-gantry ihamba kalula ngesandla ngemva kokukhipha zonke izikulufu zokuthumela. 19 Sika futhi ukhiphe amabhande enjoloba amabili abambe umgqomo kadoti ngesikhathi sokuthutha.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
20 Namathisela intambo yamandla ku-Flex bese uyixhuma odongeni. Qiniseka ukuthi indawo yedekhi ayinazihibe. Phenya iswishi yamandla ngemuva kwesokunxele serobhothi. Uma isivuliwe, i-gantry iya endaweni yayo yasekhaya futhi isikrini esithintwayo sibonisa imiyalelo eyengeziwe yokumisa.

Manje njengoba i-Flex yakho isiphumile ebhokisini futhi isilungele ukuhamba, qhubekela esigabeni se-First Run ngezansi.
2.3 Ukugijima kokuqala
Yenza ukusetha okuyisisekelo esikrinini esithintwayo ngaphambi kokuxhuma noma iyiphi enye ihadiwe ku-Flex yakho. Irobhothi lizokuqondisa ekuxhumekeni kunethiwekhi yakho yelebhu, ubuyekeze isofthiwe yakamuva, nokwenza iFlex ibe ngeyakho ngokuyinikeza igama.
Vula
Uma uvula i-Flex, ilogo ye-Opentrons izovela esikrinini esithintwayo. Ngemva kwemizuzwana embalwa, izobonisa isikrini esithi “Siyakwamukela ku-Opentrons Flex yakho”.
Isikrini sokwamukela i-Opentrons Flex. Kufanele ubone lesi sikrini kuphela lapho uqala i-Flex yakho okokuqala ngqa.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
Xhuma kunethiwekhi noma ikhompuyutha
Landela imiyalo esesikrinini esithintwayo ukuze irobhothi lakho lixhumeke ukuze lihlole ukubuyekezwa kwesofthiwe futhi lithole iphrothokholi files. Kunezindlela ezintathu zokuxhuma: i-Wi-Fi, i-Ethernet, ne-USB.

Izinketho zokuxhuma kwenethiwekhi. Udinga ukuba noxhumano lwe-inthanethi ukuze umise i-Flex. I-Wi-Fi: Sebenzisa isikrini esithintwayo ukuze uxhume kumanethiwekhi e-Wi-Fi avikelwe nge-WPA2 Personal authentication (amanethiwekhi amaningi adinga kuphela iphasiwedi ukuze ajoyine angena ngaphansi kwalesi sigaba).
Qaphela: I-Flex ayisekeli izingosi ezithunjiwe (amanethiwekhi angenalo iphasiwedi kodwa alayisha a webikhasi lokuqinisekisa abasebenzisi ngemuva kokuxhuma).
Ungakwazi futhi ukuxhuma kunethiwekhi ye-Wi-Fi evulekile, kodwa lokhu akunconyiwe.
Isexwayiso: Ukuxhuma kunethiwekhi ye-Wi-Fi evulekile kuzovumela noma ubani osebangeni lesignali yenethiwekhi ukuthi alawule irobhothi lakho le-Opentrons Flex ngaphandle kokuqinisekisa.

Uma udinga ukuxhuma kunethiwekhi ye-Wi-Fi esebenzisa ukuqinisekiswa kwebhizinisi (okuhlanganisa “i-eduroam” namanye amanethiwekhi ezemfundo afanayo adinga igama lomsebenzisi nephasiwedi), qala ngokuxhuma ku-Opentrons App nge-Ethernet noma nge-USB ukuze uqedele ukusetha kokuqala. Bese uxhuma kunethiwekhi ye-Wi-Fi yebhizinisi kuzilungiselelo zokuxhumana ze-Flex yakho. Ukuze ufinyelele izilungiselelo zenethiwekhi:

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
1. Chofoza Amadivayisi kubha eseceleni engakwesokunxele yohlelo lokusebenza lwe-Opentrons. 2. Chofoza imenyu yamachashazi amathathu () ye-Flex yakho bese ukhetha Izilungiselelo Zerobhothi. 3. Chofoza ithebhu Yenethiwekhi.
Khetha inethiwekhi yakho kumenyu eyehlayo noma khetha okuthi “Joyina enye inethiwekhi…” bese ufaka i-SSID yayo. Khetha indlela yokuqinisekisa yebhizinisi esetshenziswa inethiwekhi yakho. Izindlela ezisekelwayo yilezi:
I-EAP-TTLS ene-TLS EAP-TTLS ene-MS-CHAP v2 EAP-TTLS ene-MD5 EAP-PEAP ene-MS-CHAP v2 EAP-TLS
Ngayinye yalezi zindlela idinga igama lomsebenzisi nephasiwedi, futhi kuye ngokucushwa kwakho kwenethiwekhi kungadinga isitifiketi files noma ezinye izinketho. Bheka imibhalo ye-IT yendawo yakho noma uthinte umphathi wakho we-IT ukuze uthole imininingwane yokusethwa kwenethiwekhi yakho.
I-Ethernet: Xhuma irobhothi lakho kuswishi yenethiwekhi noma kuhabhu ngentambo ye-Ethernet. Ungakwazi futhi ukuxhuma ngqo embobeni ye-Ethernet kukhompyutha yakho, uqale ngenguqulo yesistimu yerobhothi 7.1.0.
I-USB: Xhuma ikhebula le-USB A-to-B enikeziwe embobeni ye-USB-B yerobhothi kanye nembobo evuliwe kukhompuyutha yakho. Sebenzisa ikhebula le-USB B-to-C noma i-adaptha ye-USB A-to-C uma ikhompuyutha yakho ingenayo imbobo ye-USB-A.
Ukuze uqhubeke nokusetha, ikhompuyutha exhunyiwe kufanele ibe nohlelo lokusebenza lwe-Opentrons olufakiwe futhi lusebenze. Ukuze uthole imininingwane yokufaka Uhlelo Lokusebenza Lwe-Opentrons, bheka isigaba Sokufakwa Kohlelo Lokusebenza sesahluko seSoftware kanye Nokusebenza.
Faka izibuyekezo zesofthiwe
Manje njengoba usuxhume kunethiwekhi noma ikhompuyutha, irobhothi lingahlola isofthiwe nezibuyekezo ze-firmware futhi izilande uma kudingeka. Uma kunesibuyekezo, kungase kuthathe amaminithi ambalwa ukukufaka. Uma isibuyekezo sesiqedile, irobhothi lizoqala kabusha.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
Namathisela I-Emergency Stop Pendant
Xhuma i-Emergency Stop Pendant (E-stop) efakiwe embobeni engumsizi (AUX-1 noma i-AUX-2) ngemuva kwerobhothi.

Ngaphambi nangemuva kokuxhuma i-Emergency Stop Pendant.
Ukunamathisela kanye nokwenza i-E-stop isebenze kuyisibopho sokunamathisela izinsimbi nokusebenzisa amaphrothokholi ku-Flex. Ukuze uthole ulwazi olwengeziwe ngokusebenzisa i-E-stop phakathi nokusebenza kwerobhothi, bheka isigaba se-Emergency Stop Pendant sesahluko Sencazelo Yesistimu.
Nikeza irobhothi lakho igama
Ukuqamba irobhothi lakho kukuvumela ukuthi ulikhombe kalula endaweni yakho yaselabhu. Uma unamarobhothi amaningi e-Opentrons kunethiwekhi yakho, qiniseka ukuthi uwanikeza amagama ahlukile. Uma usuliqinisekisile igama irobhothi lakho, uzoyiswa kudeshibhodi yakho ye-Opentrons Flex. Cishe isinyathelo esilandelayo ozofuna ukusithatha wukunamathisela amathuluzi, ahlanganiswe esigabeni esilandelayo.
2.4 Ukufakwa nokulinganiswa kwezinsimbi
Ngemuva kokusethwa kwerobhothi kokuqala, isinyathelo esilandelayo ukunamathisela izinsimbi irobhothi bese uzilinganisa.
Ukuze ufake ithuluzi, qala ngokuthepha Amathuluzi esikrinini esithintwayo noma uye esigabeni se-Pipettes namamojula sesikrini semininingwane yedivayisi Kuhlelo Lokusebenza lwe-Opentrons. Khetha ukukhweza okungenalutho bese ukhetha okuthi Namathisela i-Pipette noma Namathisela i-Gripper. Uma intaba ofuna ukuyisebenzisa isivele igcwele, udinga ukukhipha i-pipette noma i-gripper kuqala.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
Qaphela: Inqubo yokufaka iyonke iyafana noma ngabe usebenzisa isikrini esithintwayo noma Uhlelo Lokusebenza lwe-Opentrons. Noma iyiphi idivayisi oqala kuyo izolawula inqubo yokufaka uze uyiqede noma uyikhansele.
Uma uqala esikrinini esithintwayo, uhlelo lokusebenza luzobonisa irobhothi njengokuthi "limatasa". Uma uqala kuhlelo lokusebenza, isikrini esithintwayo sizobonisa i-modal ebonisa ukuthi ukufakwa kwensimbi kuyaqhubeka.
Inqubo yokufaka okuyiyonayona iyahlukahluka kuye ngethuluzi olinamathiselayo, njengoba kuvezwe ezigabeni ezingezansi. Wonke amathuluzi anenqubo yokulinganisa ezenzakalelayo, okufanele uyenze ngokushesha ngemva kokufakwa.
Ukufakwa kwe-pipette
Uma ufaka i-pipette, uzoqondiswa ezinyathelweni ezilandelayo kusikrini esithintwayo noma kuhlelo lokusebenza lwe-Opentrons.
1. KHETHA UHLOBO LWEPIPETTE Khetha phakathi kuka-1- noma 8-Channel Pipette kanye ne-96-Channel Pipette. Ukunamathisela i-96-Channel Pipette kudinga izinyathelo ezimbalwa ezengeziwe ngoba inamathisela epuleti elikhwezayo elikhethekile elihlanganisa kokubili izikhwebu ze-pipette.
2. LUNGISELELA UKUFAKA Khipha ilebula emphemeni bese uhlanza indawo yokusebenza ukuze wenze ukunamathisela nokulinganisa kube lula. Futhi qoqa okokusebenza okudingekayo, okufana ne-calibration probe, i-hex screwdriver, ne-mounting plate (ye-96-Channel Pipette).
3. XHUMANA FUTHI UVIKELE IPIPETTE I-gantry izohamba iye ngaphambi kwerobhothi ukuze ukwazi ukunamathisela ipayipi.
Amapayipi amapayipi angu-1- kanye no-8-Channel axhumeka ngokuqondile endaweni yokukhweza i-pipette. I-96-Channel Pipette idinga i-mounting plate. Ukuze unamathisele i-mounting plate, kuzomele uqale unqamule inqola engu-z-axis yesikhwezi se-pipette esilungile.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
Xhuma i-pipette endaweni ekhethiwe ye-pipette futhi uvikele izikrini zayo.
4. QAPHELA UKULINGANA OKUZENZAKALELAYO Ukuze ulinganise i-pipette, namathisela i-probe yokulinganisa kubhobho le-pipette elifanele. I-pipette izohamba ngokuzenzakalelayo ukuze ithinte amaphuzu athile emphemeni futhi ilondoloze lawa manani okulinganisa ukuze asetshenziswe esikhathini esizayo. Uma ukulinganisa sekuqediwe futhi usususe uphenyo, i-pipette izobe isilungele ukusetshenziswa kumaphrothokholi.
Ukufakwa kwe-Gripper
Uma ufaka i-gripper, uzoqondiswa ezinyathelweni ezilandelayo kusikrini esithintwayo noma kuhlelo lokusebenza lwe-Opentrons.
1. LUNGISELELA UKUFAKA Khipha ilebula emphemeni bese uhlanza indawo yokusebenza ukuze wenze ukunamathisela nokulinganisa kube lula. Futhi qoqa i-screwdriver ye-hex edingekayo futhi uqiniseke ukuthi iphinikhodi yokulinganisa isendaweni yayo yokugcina ku-gripper.
2. Xhuma FUTHI UVIKELE I-GRIPPER I-gantry izohamba iye ngaphambili irobhothi ukuze ukwazi ukunamathisela into yokubamba. Xhuma i-gripper ku-extension mount futhi uvikele izikulufu zayo.
3. QAPHELA UKUKHALA NGOKUZENZAKALELAYO Ukuze ulinganise isibambi, faka iphinikhodi yokulinganisa emhlathini ongaphambili. I-gripper izohamba ngokuzenzakalelayo ukuze ithinte amaphuzu athile kudekhi futhi ilondoloze lawa manani okulinganisa ukuze asetshenziswe esikhathini esizayo. Bese uphinda inqubo efanayo ngephinikhodi yokulinganisa emhlathini ongemuva. Uma ukulinganisa sekuqediwe futhi usubuyisele iphinikhodi endaweni yalo yokugcina, i-gripper izobe isilungele ukusetshenziswa kumaphrothokholi.
2.5 Ukufuduka
Lesi sigaba sihlinzeka ngezeluleko nemiyalelo mayelana nokuthi ulihambisa kanjani irobhothi lakho le-Opentrons Flex ebangeni elifushane nelide.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
Ukunyakaza okufushane
Ukunyakaza okufushane kuhlanganisa ibanga lamabanga ukusuka “asivele sikunyakazise kancane” kuye ngaphesheya kwelebhu, phansi kwehholo, noma kwesinye isitezi esakhiweni sakho. Kulezi zimo, ungahambisa i-Flex yakho ngesandla. Ukuyihambisa ngenqola yesandla nakho kuyinketho enhle.
Isexwayiso: I-Flex inesisindo esingu-88.5 kg. Ngenxa yalokho, kudinga abantu ababili ukuyiphakamisa futhi bayihambise ngokuphepha.
Namathisela kabusha izibambo zokuphakamisa ukuze uhambise i-Flex yakho endaweni entsha, eseduze. Ukuphakamisa nokuthwala iFlex ngezibambo zayo kuyindlela efanele yokuhambisa irobhothi amabanga amafushane. Susa izibambo futhi uzigcine Kukhithi Yomsebenzisi ngemva kokuqedwa kokuthutha. Ukuze uvimbele ukulimaza irobhothi, hlala usebenzisa izibambo zokuliphakamisa ukuze ulithathe futhi ulihambise. Ungathathi uhlaka ukuze uphakamise noma uhambise irobhothi lakho.
Ukunyakaza kwebanga elide
Ukuhamba ibanga elide kuthutha iFlex yakho isuke emagcekeni enyuvesi, isikhungo, noma isikhungo sakho. Ngaphesheya kwedolobha, idolobha elisha, isifunda, isifundazwe, noma izwe konke kuyi-exampokuncane kokuhamba ibanga elide. Kulokhu, uzodinga ukupakisha i-Flex ukuze uyivikele ezintweni, ukushaqeka, nokunyakaza okungamahlehla okungenzeka ngenkathi usendleleni.
Uma uye wagcina ikhreyithi yokuthumela kanye nezisekelo zangaphakathi eze ne-Flex yakho, ungayipakisha kabusha kulezi zinsiza ukuze uhambe ibanga elide. Landela izinyathelo ze-unboxing ngokulandelana ukuze ulungiselele i-Flex yakho ukunyakaza okude. Ngokuyisisekelo, kufanele:
Nqamula intambo yamandla nenethiwekhi, uma inamathiselwe. Susa zonke izingxenyekazi zekhompuyutha ezinamathiselwe nelebhu. Namathisela kabusha amapuleti edekhi. Khiya i-gantry (bona isigaba se-General Moving Advice ngezansi). Susa futhi ugcine amaphaneli ewindi.
Uma ugcine ikhreyithi langempela:
Namathisela kabusha uhlaka lokuthumela ku-Flex futhi uluvikele kusisekelo sephalethi usebenzisa amabakaki e-L. Engeza i-padding bese uhlanganisa kabusha ikhreyithi lokuthumela.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
Uma ungenalo ikhreyithi langempela kanye nokokusebenza okuhlobene, xhumana nenkampani yezokuthutha enedumela elihle. Bangakuphatha ukupakisha, ezokuthutha, nenqubo yokulethwa kwakho.
Iseluleko esijwayelekile esinyakazayo
NQAMULA AMANDLA KANYE AMAKHABULA ENENETHEKI Ngaphambi kokuhambisa i-Flex yakho, ungakhohlwa: Vala amandla futhi uwakhiphe esiphehlisweni samandla. Nqamula ikhebula le-Ethernet noma le-USB, uma lisetshenziswa.
KHIYA IGANTRI Ngaphambi kokuhambisa i-Flex yakho, phinda ufake izikulufu zokukhiya ukuze ubambe i-gantry endaweni. Izindawo zokukhiya i-gantry zitholakala: Kujantshi ongakwesokunxele eduze nangaphambili kwerobhothi. Ngaphansi kwengalo ye-gantry eqondile. Ngakwesokudla sesitimela eduze nangaphambili kwerobhothi. Ukukhiya le ngxenye ye-gantry kudinga encane
ubakaki osawolintshi nezikulufu zokukhiya ezimbili.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
EKHAYA IGANTRI Ungase ungafuni ukukhiya i-gantry uma uhambisa irobhothi endaweni eseduze. Uma unquma ukungayikhiyi, okungenani sebenzisa isikrini esithintwayo noma i-Opentrons App ukuze uthumele i-gantry endaweni yayo yasekhaya ngaphambi kokuyinciphisa. Ukuze ekhaya i-gantry ngesikrini esithintwayo, cindezela imenyu yamachashazi amathathu () bese uthinta Ikhaya Gantry. Ukuze ekhaya i-gantry ngohlelo lokusebenza lwe-Opentrons: Chofoza Amadivayisi. Chofoza ku-Flex yakho ohlwini lwamadivayisi. Chofoza imenyu yamachashazi amathathu () bese uchofoza inkinobho ethi Ikhaya.
SUSA AMAMODULE Amamojula angaphakathi kwedekhi nokunye okunamathiselwe kwengeza isisindo esengeziwe ku-Flex yakho. Ziphinde zithinte isikhungo serobhothi samandla adonsela phansi, alenza lizizwe “lithiphu” lapho liliphakamisa. Ukuze usize ukwenza lula futhi ulinganise irobhothi, susa noma yiziphi izisetshenziswa ezinamathiselwe nelebhu ngaphambi kokuthi uyithathe.
FAKA KABUSHA IZILOTHI ZAMADEKI Sincoma ukuthi uphinde unamathisele izikhala zedekhi ukuze uhambe ibanga elide. Ukuvikela izikhala ezindaweni zazo zangempela kusiza ukuvimbela ukulahleka kwengozi. Ukunamathisela kabusha izikhala zedekhi yokuhamba okufushane eduze kwelebhu kuyinketho.
POST-MOVE RECALIBRATION Kufanele ulinganise kabusha noma yimaphi amathuluzi namamojula ngemva kokuwafaka kabusha. Ukuze uthole imininingwane eyengeziwe mayelana nokulinganiswa kwamamojula, bheka isahluko samamojula.

I-OPENTRONS FLEX

ISAHLUKO 2: UKUFAKA NOKUTHUTHA
Imicabango yokugcina mayelana nokuthutha
I-Flex yakho iwumshini oqinile futhi owakhiwe kahle, kodwa futhi iyithuluzi lesayensi elinembile elakhelwe ukubiza ukubekezelelana. Ngenxa yalokho, kufanele uyiphathe ngokucophelela lapho uyithuthela endaweni yangakini yomsebenzi noma uyithumela ezweni lonke. Lokhu kusho ukulandela isiqondiso esinikezwe lapha kanye nokusebenzisa ingqondo yakho mayelana nendlela yokuthutha ucezu olubizayo lwempahla yaselabhorethri. Iphuzu elibalulekile: lapho uhambisa i-Flex yakho, yenza iphutha ohlangothini lokuqaphela kanye nokunamathisela okwengeziwe.
Uma unemibuzo noma ukukhathazeka mayelana nokuthuthwa kwe-Flex yakho, xhumana nathi ku-support@opentrons.com.

I-OPENTRONS FLEX

ISAHLUKO 3
Incazelo Yesistimu

Lesi sahluko sichaza izinhlelo zehadiwe ze-Opentrons Flex, ezisekela izici zayo eziyisisekelo zelebhu. Idekhi, i-gantry, kanye nokukhwezwa kwezinsimbi ze-Opentrons Flex kunika amandla ukusetshenziswa koketshezi olunembayo kanye nezingxenye zokuphatha i-labware. Isikrini esithintwayo esikudivayisi sinika amandla ukusebenzisa amaphrothokholi kanye nokuhlola isimo serobhothi ngaphandle kwesidingo sokuletha ikhompuyutha yakho ebhentshini laselabhu. Ukuxhumana okunezintambo nokungenantambo kunika amandla ukulawula okwengeziwe okuvela kuhlelo lokusebenza lwe-Opentrons (bona Isofthiwe Nesahluko Sokusebenza ukuze uthole imininingwane eyengeziwe) nokwelula izici zesistimu ngokunamathisela ama-peripheral (bona isahluko samamojula).

3.1 Izingxenye zomzimba

Ikhamera

Ukukhanya Kwesimo

Isikrini sokuthinta

Uhlaka

I-Gantry Deck
Umnyango Wangaphambili
Izindawo zezingxenye zomzimba ze-Opentrons Flex.

I-Side Windows Handle Caps

I-OPENTRONS FLEX

ISAHLUKO 3: INCAZELO YOHLELO

Uhlaka kanye nesivalo
Uhlaka lwerobhothi le-Opentrons Flex luhlinzeka ngokuqina nokusekelwa kwesakhiwo sedekhi yalo kanye ne-gantry. Wonke amasistimu angaphansi kamishini akhiwe futhi agibele kuhlaka oluyinhloko. Uhlaka lwakhiwe ngokuyinhloko ngeshidi lensimbi ne-aluminium extrusions.
Uhlaka lwensimbi lunezimbobo zamafasitela aseceleni kanye nomnyango ongaphambili owenziwe nge-polycarbonate ekhanyelayo ekuvumela ukuthi ubone ukuthi kwenzekani ngaphakathi kwe-Flex. Amahinji omnyango wangaphambili avulekile ukuze kungene ingaphakathi lesistimu. Ngomnyango wangaphambili ovulekile, ungakwazi ukunamathisela amathuluzi, amamojula, nezakhiwo zedekhi; lungisa idekhi ngaphambi kwephrothokholi; noma ulawule isimo sedekhi phakathi nephrothokholi.
Imichilo ye-LED emhlophe emaphethelweni angaphakathi aphezulu ohlaka inikeza ukukhanya kwe-ambient okulawulwa yisofthiwe. Ikhamera engu-2-megapixel ingathwebula idekhi nendawo yokusebenza ukuze irekhodwe futhi ilandele umkhondo wokwenziwa kwephrothokholi.

Ikhishi nendawo yokusebenza
Idekhi iyindawo ye-aluminium eyenziwe ngomshini lapho kusetshenziswa khona izivumelwano zesayensi ezizenzakalelayo. Idekhi inezikhala eziyi-12 eziphambili zefomethi ye-ANSI/SLAS ezingalungiselelwa kabusha ukuze zibambe i-labware, amamojula, nezinto ezisebenzisekayo. Izikhala zedekhi zikhonjwa ngesistimu yokuxhumanisa, ene-slot A1 ngemuva kwesokunxele kanye ne-slot D3 ngaphambili kwesokudla.

Ukunwetshwa Slot (for Thermocycler) Indawo yokusebenza

StagIndawo

Izindawo zedekhi ngaphakathi kweFlex.

I-OPENTRONS FLEX

ISAHLUKO 3: INCAZELO YOHLELO
Indawo yokusebenza yindawo ebonakalayo ngaphezu kwedekhi efinyeleleka ngamapayipi. Ilebhu ebekwe ezikhaleni A1 kuya ku-D3 isendaweni yokusebenza.
I-Opentrons Flex iza nezikhala zedekhi ezikhiphekayo kuzo zonke izikhundla eziyi-12 endaweni yokusebenza. Imbobo ngayinye yedekhi ineziqeshana zelebhu ekhoneni zokubeka i-labware ngokuphephile endaweni.
Ungakwazi ukulungisa kabusha idekhi ngokushintsha izikhala ngokufaka ezinye izinto ezilungiswayo, okuhlanganisa udoti ogudlukayo, i-waste chute, nama-module caddies. Imbobo enwetshiwe engemuva kwe-A1 isetshenziswa kuphela ukwenza igumbi elengeziwe le-Thermocycler Module, ethatha izikhala ze-A1 ne-B1.
Qaphela: Izikhala zedekhi ziyashintshana phakathi kwekholomu (1, 2, noma 3) kodwa hhayi kuwo wonke amakholomu; Ikholomu 1 kanye nekholomu 3 Izikhala ziyizingcezu ezihlukene naphezu kobukhulu bazo obufanayo. Ungakwazi ukubona ukuthi iyiphi ikholomu i-slot engena ngokuqondisa isiqeshana se-labware eluhlaza okwesibhakabhaka ngemuva kwesokunxele.
Kufanele ushiye izikhala zedekhi zifakwe ezindaweni lapho ufuna ukubeka i-labware ezimele. Idekhi nezinto ezibekwe kuyo zihlala zimile, ngaphandle uma kushukunyiswa into yokubamba noma ukungenelela okwenziwa ngesandla.
Stagindawo
I-stagindawo ye-ing iyindawo eyengeziwe ngakwesokudla kwedekhi. Ungagcina ilebula kule ndawo ngemuva kokufaka i-staging indawo Slots. Ilebhu ibekwe Slots A4 ngokusebenzisa D4 kukhona stagindawo. I-Flex pipettes ayikwazi ukufinyelela ku-stagIndawo, kodwa i-gripper ingakwazi ukucosha bese ihambisa ilebula ukuya nokusuka kule ndawo. Ukwengeza izikhala ezengeziwe kusiza ukugcina indawo yokusebenza itholakala kumishini esetshenziswa kumaphrothokholi akho azenzakalelayo.
Stagizindawo zokuhlala zifakiwe ekucushweni okuthile kwendawo yokusebenza futhi ziyatholakala futhi ukuze zithengwe ku-https://shop.opentrons.com.

I-OPENTRONS FLEX

ISAHLUKO 3: INCAZELO YOHLELO

Staging Indawo nge Slots Kufakiwe

Izakhiwo zedekhi
Izinto ezilungiswayo ziyizinto zehadiwe ezithatha indawo yezikhala zedekhi ejwayelekile. Bakuvumela ukuthi wenze ngendlela oyifisayo ukwakheka kwedekhi futhi wengeze ukusebenza ku-Flex yakho. Njengamanje, ukulungiswa kwedekhi kufaka phakathi i-stagizindawo zokubeka izikhala, umgqomo kadoti wangaphakathi, kanye ne-chute yangaphandle kadoti. Ungafaka kuphela izinto zokulungisa ezindaweni ezimbalwa ezithile zedekhi. Ithebula elilandelayo libala izindawo zedekhi zomdlalo ngamunye.

Ukulungiswa kwe-Staging indawo Slots Udoti bin Udoti chute Udoti chute nge stagindawo slot

Izikhala A3D3 A1D1 kanye A3-D3 D3 D3 kuphela

Ukulungiswa akunamandla. Aziqukethe izingxenye ze-elekthronikhi noma zemishini ezixhumanisa isimo sazo samanje nendawo yedekhi nerobhothi. Lokhu kusho ukuthi kufanele usebenzise isici sokumisa idekhi ukuze wazise iFlex ukuthi yiziphi izinto ezinamathiselwe emphemeni nokuthi zitholakala kuphi.

I-OPENTRONS FLEX

ISAHLUKO 3: INCAZELO YOHLELO

Ungafinyelela izilungiselelo zokucushwa kwedekhi esikrinini esithintwayo ngemenyu enamachashazi amathathu () nakuhlelo lokusebenza lwe-Opentrons. Bheka isigaba sokucushwa kweDekhi sesahluko seSofthiwe kanye Nokusebenza ukuze uthole ulwazi lokuthi ungayilungisa kanjani idekhi kusukela kusikrini esithintwayo.

Waste chute
I-Opentrons Flex Waste Chute idlulisa uketshezi, amathiphu, ama-tip rack, namapuleti emithombo kusuka endaweni ebiyelwe ye-Flex kuya emgqonyeni kadoti obekwe ngaphansi kokuvuleka kwawo kwangaphandle. I-chute kadoti inamathela ku-adaptha yepuleti ledekhi elingena ku-slot D3. Iphuma nephaneli yefasitela eliyisipesheli elinguhhafu elivumela i-chute ukuthi inwebe iphume ngaphambili kwerobhothi.
Izingxenye ze-chute yemfucuza.

Ikhava ye-adaptha ye-Deck Plate

Waste Chute
I-adaptha ye-Deck Plate ene-StagIndawo

Staging indawo Slots
Stagizindawo zokubeka izikhala ziyizicucu zedekhi ezihambisanayo ze-ANSI/SLAS ezithatha indawo yezikhala ezijwayelekile kukholamu 3 futhi zengeze izikhala ezintsha ku-s.tagindawo - konke ngaphandle kokulahlekelwa isikhala endaweni yokusebenza. Ungafaka imbobo eyodwa noma umkhawulo wezikhala ezine ukuze udale ikholomu entsha (A4 ukuya ku-D4) ngakwesokudla kwedekhi. Qaphela, nokho, ukuthi ukufaka indawo yedekhi ye-A3 kudinga ukuhambisa umgqomo kadoti. Ngokungeza u-stagizindawo zokubeka indawo emphemeni, irobhothi lakho le-Flex lingagcina i-labware eyengeziwe futhi lisebenze ngokuphumelelayo.

I-OPENTRONS FLEX

I-Flex stagindawo slot.
49

ISAHLUKO 3: INCAZELO YOHLELO
UKUFAKWA KWESIKHALA
Ukufaka, susa izikulufu ezinamathisela i-slot evamile endaweni bese uyishintsha ngo-stagindawo slot. Ngemva kokufaka, sebenzisa isikrini esithintwayo noma Uhlelo lokusebenza lwe-Opentrons ukuze utshele irobhothi oyengezile njengetagindawo yokubeka endaweni yokupaka.
Ifaka njengetagindawo slot.

UKUHAMBISANA KWESIKHALA Staging indawo Slots iyahambisana Flex amathuluzi, amamojula, kanye labware ohlwini ngezansi.

Flex ingxenye Gripper Pipettes Amamojula
Ilebhu

Stagukuhambisana kwendawo
I-Flex Gripper ingahambisa ilebhu iye noma isuke ku-staging indawo Slots.
I-Flex pipettes ayikwazi ukufinyelela ku-stagindawo. Sebenzisa i-gripper ukuhambisa ama-tip racks kanye ne-labware kusuka ku-stagindawo yokusebenzela endaweni yokusebenza ngaphambi kokufakwa kwamapayipi.
I-Magnetic Block GEN1 ingafakwa kukholamu 3 phezu kokuthitagindawo slot. Amamojula awasekelwe kukholomu yesi-4.
Amamojula anamandla afana ne-Heater-Shaker kanye ne-Temperature Module angena kuma-caddy angafakwa kukholamu 3. Awukwazi ukwengeza ngokuthitagindawo yokubeka indawo endaweni ehlala i-module caddy.
Stagizikhala zendawo ezinobukhulu obufanayo be-ANSI/SLAS njengezikhala zedekhi ezijwayelekile. Sebenzisa ilebula ehambisana ne-gripper ku-stagendaweni, noma engeza ngesandla futhi ususe ilebula kule ndawo.

I-OPENTRONS FLEX

ISAHLUKO 3: INCAZELO YOHLELO
Uhlelo lokunyakaza
Okunamathiselwe ohlakeni i-gantry, okuyisistimu yerobhothi yokuhamba nokubeka indawo. I-gantry ihamba ngokuhlukene eduze kwe-eksisi ka-x- kanye no-y ukuze imise amapayipi ne-gripper ezindaweni eziqondile zokwenziwa kwephrothokholi. Ukunyakaza kulawa ma-eks kunembe ngqo ku-0.1 mm eseduze. I-gantry ilawulwa yi-36 VDC hybrid bipolar stepper motors. Ngokulandelayo, okunamathiselwe ku-gantry kukhona izikhwebu ze-pipette kanye ne-extension mount. Lokhu kuhamba ku-axis engu-z ukuze kumiswe amapayipi kanye nokubamba ezindaweni eziqondile zokusetshenziswa kwephrothokholi. Ukuhamba kule eksisi kulawulwa ama-motor angu-36 e-VDC hybrid bipolar stepper. I-electronics equkethwe ku-gantry inikeza amandla angu-36 e-VDC nokuxhumana kuma-pipettes kanye ne-gripper, uma enamathiselwe.
I-Gantry

I-Pipette Mounts

I-Extension Mount

Indawo yensimbi ifakwa ku-Flex.

Isikrini sokuthinta kanye nezibonisi ze-LED
Umsebenzisi oyinhloko yisikrini esithintwayo se-LCD esingu-7 intshi, esitholakala ngaphambili kwesokudla serobhothi. Isikrini esithintwayo simbozwe nge-Gorilla Glass 3 yokumelana nokuklwebheka nokulimala. Finyelela izici eziningi ze-Flex khona kanye esikrinini esithintwayo, okuhlanganisa:

I-OPENTRONS FLEX

ISAHLUKO 3: INCAZELO YOHLELO

Ukuphathwa kwephrothokholi Ukusethwa kwephrothokholi, ukwenza, nokuqapha Ukuphathwa kwelebhu Izilungiselelo zerobhothi Isofthiwe yesistimu kanye ne-firmware ibuyekeza amalogi okusebenza nezaziso zamaphutha
Ukuze uthole ulwazi olwengeziwe ngokusebenzisa i-Flex ngesikrini esithintwayo, bheka isigaba sokuSebenza kwesikrini sokuthinta sesahluko se-Software and Operation.
Isibani sesimo siwuchungechunge lwama-LED ngaphezulu ngaphambili kwerobhothi olunikeza ulwazi lapho uthi shazi mayelana nerobhothi. Imibala ehlukene kanye namaphethini okukhanyisa angakhuluma ngempumelelo ehlukahlukene, ukwehluleka, noma izimo ezingasebenzi:

Umbala we-LED White Neutral states
Green Normal states
I-Blue Mandatory ithi i-Yellow Abnormal states i-Red Emergency states

Iphethini ye-LED Solid Pulsing
Icwayiza kabili
I-Solid Pulsing Pulsing

Isimo serobhothi
Ivuliwe futhi ayisebenzisi iphrothokholi Irobhothi limatasa (isb, ukubuyekeza isofthiwe noma i-firmware, ukumisa ukusebenza kwephrothokholi, ukukhansela ukusebenza kwephrothokholi) Isenzo siqediwe (isb., iphrothokholi egciniwe, isofthiwe ibuyekeziwe, ithuluzi elinamathiselwe noma lisusiwe) Iphrothokholi iyasebenza. Iphrothokholi imisiwe

Okuqinile

Iphutha lesofthiwe

Icwayiza kathathu, iphindelela

Iphutha elibonakalayo (isb, ukuphahlazeka kwensimbi)

Isibani sesimo singaphinda sikhutshazwe kuzilungiselelo zerobhothi.

I-OPENTRONS FLEX

ISAHLUKO 3: INCAZELO YOHLELO
3.2 Amapayipi
Ama-Opentrons pipette angamathuluzi alungisekayo asetshenziselwa ukuhambisa uketshezi kuyo yonke indawo yokusebenza ngesikhathi sokwenziwa kwezivumelwano. Kunamapayipi amaningana e-Opentrons Flex, angakwazi ukuphatha amavolumu ukusuka ku-1 µL kuye ku-1000 µL eziteshini ezingu-1, 8, noma ezingu-96:
I-Opentrons Flex 1-Channel Pipette (1 µL) i-Opentrons Flex 50-Channel Pipette (1 µL) Opentrons Flex 5-Channel Pipette (1000 µL) Opentrons Flex 8-Channel Pipette (1 µL) Opentrons 50 Flex 8x5x1000
Amapayipi anamathisele ku-gantry esebenzisa izikulufo ezithunjiwe ngaphambili kwe-pipette. I-1-channel kanye ne-8-channel pipettes ngayinye ithatha indawo eyodwa ye-pipette (kwesokunxele noma kwesokudla); i-piette ye-96-channel ithatha zombili izintaba. Ukuze uthole imininingwane ngokufaka ama-pipette, bheka Ukufakwa Kwensimbi Nokulinganiswa.

Okunamathiselwe Okuthunjiwe
Izikulufu

Izikulufo Ezinamathiselwe Ezithunjiwe

I-Ejector

Imilomo (O-rings eshintshwayo)

Imilomo (Fixed O-rings)

Izindawo zezingxenye zamapayipi amapayipi angu-1-, 8-, kanye namashaneli angu-96.

I-Ejector

I-OPENTRONS FLEX

ISAHLUKO 3: INCAZELO YOHLELO

Ama-pipette athatha amathiphu epulasitiki alahlwayo ngokuwacindezela emibhobho ye-pipette, bese esebenzisa amathiphu ukufisa nokukhipha uketshezi. Inani lamandla adingekayo ukuze kulandwe liyakhuphuka njengoba amathiphu engeziwe ethathwa kanyekanye. Ukuze uthole izinombolo ezincane zamathiphu, i-pipette inamathisela amathiphu ngokuphusha umbhobho ngamunye we-pipette phansi uthiphu. Ukuze kuzuzwe amandla adingekayo wokucosha i-rack egcwele yamathiphu, i-pipette ye-96-channel iphinde idonsele amathiphu phezulu kuma-nozzles. Lesi senzo sokudonsa sidinga ukubeka ama-tip rack ku-adaptha ye-tip rack, kunokuba ngokuqondile endaweni yedekhi. Ukuze ulahle amathiphu (noma uwabuyisele ku-rack yawo), indlela yokukhipha i-pipette iphusha amathiphu asuke emibhobho.

Imininingwane ye-Pipette
Amapayipi e-Opentrons Flex aklanyelwe ukuphatha izinhlobo eziningi zamavolumu. Ngenxa yobubanzi bawo bebonke ububanzi, bangasebenzisa osayizi abaningi bamathiphu, athinta izici zabo zokuphatha uketshezi. I-Opentrons ihlole amapayipi e-Flex ngokunemba nokunemba enanini lethiphu nenhlanganisela yevolumu yoketshezi:

Pipette
I-Flex 1-Channel
50µL
I-Flex 1-Channel
1000µL
I-Flex 8-Channel
50µL
I-Flex 8-Channel
1000µL

Ithiphu Umthamo 50 µL 50 µL 50 µL 50 µL 50 µL 200 µL 1000 µL 50 µL 50 µL 50 µL 50 µL 50 µL 200 µL 1000

Ivolumu Ehloliwe 1 µL 10 µL 50 µL 5 µL 50 µL
200µL 1000µL
1 µL 10 µL 50 µL 5 µL 50 µL 200 µL 1000 µL

Ukunemba %D 8.00% 1.50% 1.25% 5.00% 0.50% 0.50% 0.50% 10.00% 2.50% 1.25% 8.00% 2.50% 1.00% 0.70%

Precision %CV 7.00% 0.50% 0.40% 2.50% 0.30% 0.15% 0.15% 8.00% 1.00% 0.60% 4.00% 0.60% 0.25% 0.15%

I-OPENTRONS FLEX

ISAHLUKO 3: INCAZELO YOHLELO

I-Flex 96-Channel
1000µL

50 µL 50 µL 200 µL 1000 µL

5 µL 50 µL 200 µL 1000 µL

10.00% 2.50% 1.50% 1.50%

5.00% 1.25% 1.25% 1.50%

Gcina lolu lwazi lokunemba engqondweni lapho ukhetha amathiphu we-pipette yakho. Ngokuvamile, ukuze uthole imiphumela ehamba phambili kufanele usebenzise amathiphu amancane ahlangabezana nezidingo zephrothokholi yakho.

Qaphela: I-Opentrons yenza ukuhlolwa kwe-volumetric yamapayipi e-Flex ukuze kuqinisekiswe ukuthi ahlangabezana nokunemba nokunemba kwemininingwane ebhalwe ngenhla. Awudingi ukulinganisa ivolumu ama-pipettes akho ayikhiphayo ngaphambi kokusetshenziswa. Kufanele wenze ukulinganisa kwendawo. Bheka isigaba esilandelayo, kanye nesigaba Sokufakwa kwe-Pipette sesahluko sokufaka nokuthuthela kwenye indawo, ukuze uthole imininingwane.
Izinsizakalo ze-Opentrons Care kanye ne-Opentrons Care Plus zihlanganisa ukushintshwa kwe-pipette minyaka yonke kanye nezitifiketi zokulinganiswa. Bheka isigaba se-Service Flex sesahluko se-Maintenance and Service ukuze uthole imininingwane.

Ukulinganisa kwe-Pipette
Ikhithi Yomsebenzisi ihlanganisa uphenyo lokulinganisa i-pipette yensimbi, oyisebenzisayo ngesikhathi sokulinganiswa kwendawo. Ngesikhathi sokusebenza kwephrothokholi, gcina ngokuphephile i-probe kusibambi kazibuthe ensikeni engaphambili yerobhothi. Ngesikhathi senqubo yokulinganisa, namathisela i-probe ku-nozzle efanele futhi uyikhiye endaweni. Irobhothi lihambisa i-probe ezindaweni zokulinganisa edekhini ukuze likale indawo eqondile ye-pipette.
I-adaptha yokubeka ithiphu ye-Pipette
Imikhumbi ye-Opentrons Flex 96-channel pipette enama-adaptha ama-tip rack adapter. Lawa amabakaki e-aluminium akhiwe ngokunemba owabeka edekhini. Ama-adaptha abamba ama-racks we-Flex 50 L, 200 L, kanye no-1000 µL.

I-OPENTRONS FLEX

ISAHLUKO 3: INCAZELO YOHLELO
Ngenxa yamandla ahilelekile, i-pipette yesiteshi esingu-96 idinga i-adaptha ukuze inamathisele i-tip rack egcwele kahle. Ngesikhathi senqubo yokunamathisela, i-pipette ihamba phezu kwe-adaptha, izehlisele ezikhonkwaneni ezifakwayo, bese idonsa amathiphu kuma-pipette ngokuphakamisa i-adaptha kanye ne-tip rack. Ukudonsa amathiphu, kunokuphusha, kunikeza amandla adingekayo ukuze uvikele amathiphu kuma-pipette futhi kuvimbele ukusonteka kwendawo yedekhi. Uma isiqediwe, i-pipette yeziteshi ezingu-96 yehlisa i-adaptha kanye ne-tip rack engenalutho phezu kwedekhi. Bona isigaba samathiphu namathiphu sesahluko se-Labware ukuze uthole ulwazi olwengeziwe.
Ukuthatha ithiphu elincane
I-pipette yeziteshi ezingu-96 ingathatha irekhi egcwele yamathiphu noma inombolo encane yamathiphu. Lokhu kwandisa inani lezinhlelo zokusebenza ongazenza nge-pipette yeziteshi ezingu-96, njengoba ithatha kokubili izikhwebu ze-pipette.
Okwamanje, i-pipette yeziteshi ezingu-96 isekela ukulandwa kwethiphu ngokwengxenye yamathiphu angu-8 kusakhiwo sekholomu. Kulokhu kumisa, i-pipette isebenzisa imibhobho yayo yesobunxele kakhulu ukuze icoshe amathiphu ukusuka kwesokudla kuye kwesokunxele ku-tip rack, noma imibhobho yayo engakwesokudla kakhulu ukuze icoshe amathiphu ukusuka kwesokunxele kuye kwesokudla ku-tip rack.
Uma ucosha amathiphu angaphansi kuka-96 ku-tip rack, i-rack kufanele ibekwe ngqo edeskini, hhayi ku-adaptha yokubeka ithiphu.
Izinzwa ze-Pipette
Amapayipi e-Opentrons Flex anenani lezinzwa ezithola futhi zirekhode idatha mayelana nesimo se-pipette nanoma imaphi amathiphu ewathathile.
IZInzwa ZAmandla
Ngokuhambisana ne-probe yensimbi noma ithiphu ye-conductive, izinzwa ze-capacitance zibona lapho i-pipette ithintana nento ethile. Ukutholwa kokuxhumana phakathi kwe-probe yensimbi kanye nedekhi kusetshenziswa ekulinganisweni kwe-pipette okuzenzakalelayo kanye nezinqubo zokulinganisa imojula.
Amapayipi esiteshi esingu-1 anenzwa eyodwa ye-capacitance, kuyilapho ama-pipette eziteshi eziningi anamabili: eziteshini 1 no-8 zamapayipi angu-8 wesiteshi, naseziteshini 1 no-96 (izikhundla A1 kanye ne-H12) ze-pipette yamashaneli angu-96.

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ISAHLUKO 3: INCAZELO YOHLELO

IZInzwa ZEPHUBULO ZOKUBONA
Iswishi ye-photointerruptor ithola indawo yomshini wokukhipha ithiphu le-pipette, iqinisekisa ukuthi amathiphu athathwe noma awiswa ngempumelelo yini. 1-channel, 8-channel, kanye 96-channel pipettes wonke anenzwa eyodwa yokubona eqapha okunamathiselwe ithiphu kuzo zonke iziteshi.
I-Pipette firmware updates
I-Opentrons Flex ibuyekeza ngokuzenzakalelayo i-firmware ye-pipette ukuze iyigcine ivumelana nenguqulo yesofthiwe yerobhothi. Izibuyekezo ze-firmware ze-Pipette ngokuvamile ziyashesha, futhi zenzeka noma nini:
Unamathisele i-pipette. Irobhothi liqala kabusha.
Uma, nganoma isiphi isizathu, i-pipette firmware yakho nezinguqulo zesofthiwe yerobhothi ziphuma ekuvunyelanisweni, ungakwazi ukubuyekeza i-firmware kuhlelo lokusebenza lwe-Opentrons.
1. Chofoza Amadivayisi. 2. Chofoza i-Flex yakho ohlwini lwamadivayisi. 3. Ngaphansi Kwamathuluzi Namamojula, i-pipette engaphandle kokuvumelanisa izobonisa ukufundwa kwesibhengezo esiyisixwayiso
"Isibuyekezo se-Firmware siyatholakala." Chofoza okuthi Buyekeza manje ukuze uqale isibuyekezo.
Ungakwazi view inguqulo ye-firmware efakiwe njengamanje yanoma iyiphi i-pipette enamathiselwe. Esikrinini esithintwayo, hamba ku-Instruments bese uthinta igama le-pipette. Kuhlelo lokusebenza lwe-Opentrons, thola ikhadi le-pipette ngaphansi kokuthi Amathuluzi Namamojula, chofoza imenyu yamachashazi amathathu (), bese uchofoza okuthi Mayelana ne-pipette.

3.3 I-gripper
I-gripper ihambisa i-labware kuyo yonke indawo yokusebenza kanye ne-stagindawo ngesikhathi sokwenziwa kwamaphrothokholi. I-gripper inamathela ekukhuphukeni kwesandiso, esihlukene nezintaba ze-pipette; i-gripper ingasetshenziswa nanoma yikuphi ukucushwa kwe-pipette. Ukuze uthole imininingwane yokufaka i-gripper, bheka Ukufakwa Kwensimbi Nokulinganisa.
I-gripper ingahambisa ilebula edekhini ingene noma ivale amamojula. I-gripper ingakwazi ukushintsha amapuleti athile agcwele isiketi esigcwele ngokugcwele, amapuleti emithombo ejulile, nama-tip rack. Ukuze uthole imininingwane eyengeziwe ngokuthi iyiphi ilelebhu engakwazi ukuyihambisa, bheka I-Labware kanye nengxenye ye-Opentrons Flex Gripper yesahluko se-Labware, noma uthintane ne-Opentrons Labware Library.

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ISAHLUKO 3: INCAZELO YOHLELO

Imininingwane ye-Gripper
Imihlathi yenza ukunyakaza okuyinhloko kwe-gripper, okuwukuvula noma ukuvala ama-paddles amabili ahambisanayo ukuze kusetshenziswe noma ukukhulula amandla ezinhlangothini ze-labware. Ukunyakaza kwemihlathi kulawulwa i-36 VDC brushed motor exhunywe kusistimu yegiya le-rack-and-pinion.
Ukuhambisa ucezu lwe-labware olubanjwe imihlathi, i-gantry iphakamisa i-gripper eduze kwe-axis engu-z, iyinyakazise eceleni, bese iyayehlisa endaweni entsha ye-labware.
Izindawo zezingxenye ze-gripper.

Izikulufo zokunamathisela
Iphinikhodi yokulinganisa Izigwedlo zemihlathi

Ukulinganisa kwe-Gripper
I-gripper ihlanganisa iphinikhodi yokulinganisa yensimbi. Iphinikhodi yokulinganisa itholakala endaweni yokugcina ehlehlisiwe engxenyeni engezansi ye-gripper. Uzibuthe ubamba iphinikhodi. Ukuze ususe iphinikhodi yokulinganisa, yibambe ngeminwe yakho bese uyidonsa ngobumnene. Ukushintsha iphinikhodi, yibuyisele endaweni yokugcina. Uzokwazi ukuthi ivikelekile uma ingena endaweni.
Lapho ulinganisa i-gripper, namathisela iphinikhodi emhlathini ngamunye ngokushintshana. Irobhothi lihambisa iphinikhodi kumaphoyinti okulinganisa kudekhi ukuze likale indawo yokubamba okuyiyonayona.
Ngesikhathi sokusebenza kwephrothokholi, beka iphinikhodi endaweni yayo yokugcina ukuze igcinwe. Xhumana nathi ku-support@opentrons.com uma ulahlekelwa iphinikhodi yokulinganisa.

Izibuyekezo ze-firmware ze-Gripper
I-Opentrons Flex ibuyekeza ngokuzenzakalelayo i-firmware ye-gripper ukuze iyigcine ivumelana nenguqulo yesofthiwe yerobhothi. Izibuyekezo ze-firmware ye-Gripper ngokuvamile ziyashesha, futhi zenzeka noma nini:

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ISAHLUKO 3: INCAZELO YOHLELO
Unamathisele i-gripper. Irobhothi liqala kabusha.
Uma, nganoma isiphi isizathu, i-firmware yakho ye-gripper kanye nezinguqulo zesofthiwe yerobhothi ziphuma ekuvunyelanisweni, ungakwazi ukubuyekeza i-firmware kuhlelo lokusebenza lwe-Opentrons.
1. Chofoza Amadivayisi. 2. Chofoza i-Flex yakho ohlwini lwamadivayisi. 3. Ngaphansi Kwamathuluzi Namamojula, into yokubamba engaphandle kokuvumelanisa izobonisa ukufundwa kwesibhengezo esiyisixwayiso
"Isibuyekezo se-Firmware siyatholakala." Chofoza okuthi Buyekeza manje ukuze uqale isibuyekezo.
Ungakwazi view inguqulo ye-firmware efakiwe njengamanje ye-gripper. Esikrinini esithintwayo, hamba kokuthi Amathuluzi bese uthinta into yokubamba. Kuhlelo lokusebenza lwe-Opentrons, thola ikhadi le-gripper ngaphansi kokuthi Amathuluzi namamojula, chofoza imenyu yamachashazi amathathu (), bese uchofoza okuthi Mayelana nokubamba.
3.4 I-Emergency Stop Pendant
I-Emergency Stop Pendant (E-stop) iyinkinobho yehadiwe ezinikele yokumisa ngokushesha ukunyakaza kwerobhothi. I-Opentrons Flex idinga i-E-stop esebenzayo, engaxhunywanga ukuze inanyathiselwe ngaso sonke isikhathi. Uma ucindezela inkinobho yokumisa, i-Flex ikhansela noma iyiphi iphrothokholi esebenzayo noma ukusethwa kokuhamba komsebenzi ngokushesha ngangokunokwenzeka futhi ivimbele ukunyakaza kwamarobhothi amaningi.
Isetshenziswa nini i-E-stop
Ungase udinge ukucindezela i-E-stop:
Uma kunengozi eseduze yokulimala noma ukulimala kumsebenzisi. Lapho kunengozi eseduze yokulimala irobhothi noma enye ihadiwe. Lapho sampama-les noma ama-reagents asengozini eseduze yokungcola. Ngemva kokushayisana kwehadiwe.
Okufanelekile akufanele nanini ucindezele i-E-stop (ngaphandle ngesikhathi sokuhlolwa kwekhwalithi yehadiwe okungajwayelekile).
Ungasebenzisi i-E-stop ukuze ukhansele imisebenzi evamile, elindelekile. Kunalokho, sebenzisa inkinobho yesofthiwe esikrinini esithintwayo noma kuhlelo lokusebenza lwe-Opentrons. Ukumisa isikhashana usebenzisa isofthiwe kuzokuvumela ukuthi uqalise kabusha noma ukhansele umthetho olandelwayo, kuyilapho ukucindezela i-E-stop njalo kukhansela iphrothokholi ngokushesha.

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ISAHLUKO 3: INCAZELO YOHLELO

Ukubandakanya nokukhulula i-E-stop
I-E-stop inomshini wokucindezela ukuze uhlanganyele, ukusonta ukuze ukhulule.
Zibandakanye: Cindezela phansi ngokuqinile inkinobho ebomvu. I-Flex izongena esimweni sokumisa. Xazulula: Uma usumisiwe, lungisa ngokuphepha noma yiziphi izinkinga endaweni yokusebenza, njengokususa ukuchitheka kwegazi,
ukususa i-labware, noma ukuhambisa i-gantry (kufanele ihambe ngokukhululeka futhi kalula ngesandla). Khipha: Phendula inkinobho ngokwewashi. Izovela endaweni yayo yokungahlangani. Setha kabusha: Esikrinini esithintwayo noma kuhlelo lokusebenza lwe-Opentrons, qinisekisa ukuthi usukulungele ukuthi i-Flex iqalise kabusha
umnyakazo. I-gantry izobuyela endaweni yayo yasekhaya futhi umsebenzi wemojuli uzoqala kabusha.
Esimeni sokumiswa, i-Flex ne-hardware exhunyiwe izoziphatha ngale ndlela elandelayo:

Hardware Gantry Pipettes
I-Gripper
I-Heater-Shaker Module
I-Temperature Module Thermocycler Module Status light Isikrini sokuthinta

Ukuziphatha
Ukunyakaza okuzenzakalelayo okuvundlile kumisiwe. Ukunyakaza okuvundlile okwenziwa ngesandla kuvunyelwe.
Ukunyakaza okuqondile kwama-pipettes kumisiwe. Amabhuleki e-motor kuma-ax aqondile ayabanjwa ukuze avinjwe
ama-pipettes ekuweni. Ukunyakaza kwe-plunger nokuthatha ithiphu kumisiwe.
Ukunyakaza okuqondile kwe-gripper kumisiwe. I-motor brake ku-eksisi eqondile ibambe iqhaza ukuvimbela
into yokubamba ekuweni. Amamotho womhlathi anamandla okubamba ahlala evuliwe, ngakho-ke
i-gripper ngeke iwise i-labware okungenzeka iyithwele.
I-shaker iyama futhi ibuyele emakhaya. I-latch yelebhu iyavuleka. Ukushisisa kukhutshaziwe.
Ukushisisa noma ukupholisa kukhutshaziwe.
Ukushisisa noma ukupholisa kukhutshaziwe.
Ukukhanya kuba bomvu.
Umlayezo wokukhansela uthatha isikrini. Isibonisi esisesikrinini sibonisa ukuthi uphumelele nini
unqamule inkinobho yokumisa.

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ISAHLUKO 3: INCAZELO YOHLELO
3.5 Izixhumanisi

Vula/Vala Iswishi

Amakhava Eceleni

Izimbobo ze-USB-A

I-IEC Power Inlet

Izimbobo AUX-1, AUX-2, USB-B, Ethernet

Ukuxhumeka kwamandla
I-Opentrons Flex ixhuma kumthombo wamandla nge-IEC-C14 inlet evamile. Irobhothi liqukethe ugesi we-AC/DC wobubanzi obugcwele, lamukela i-100 VAC, okokufaka okungu-240/50 Hz futhi likuguqulele ku-60 VDC. Zonke ezinye izinto zikagesi zangaphakathi zinikwa amandla yi-36 VDC supply.

Isexwayiso: Sebenzisa kuphela intambo yamandla enikezwe irobhothi. Ungayisebenzisi intambo yamandla engenawo amandla anele noma ivolumutage izilinganiso.
Gcina intambo kagesi ingenazihibe ukuze ukwazi ukuyisusa uma kunesidingo.

Kukhona nebhethri yeselula enguhlamvu lwe-CR1220 ukunika amandla iwashi lesikhathi sangempela serobhothi uma lingaxhumekile kumandla amakhulu. Ibhethri itholakala ngaphakathi kwendawo ebiyelwe isikrini esithintwayo. Xhumana ne-Opentrons Support ukuze uthole ulwazi olwengeziwe uma ucabanga ukuthi udinga ukushintsha ibhethri.

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ISAHLUKO 3: INCAZELO YOHLELO
I-USB nokuxhumana okuyisizayo
I-Opentrons Flex inezimbobo ze-USB eziphelele ezingu-10 ezitholakala ezindaweni ezihlukahlukene zerobhothi, ezisebenza ngezinhloso ezahlukene.
Izimbobo ze-USB-A ezingemuva ezingu-8 (ezinezinombolo ze-USB-1 nge-USB-8) kanye nezinsimbi ezingu-2 (izixhumi ze-M12 ezinezinombolo ezingu-AUX-1 kanye ne-AUX-2) ezenzelwe ukuxhuma amamojula nezinsiza ze-Opentrons. Bona isahluko samamojula ukuze uthole ulwazi olwengeziwe mayelana nokuxhuma lawa madivayisi futhi uwasebenzise kumaphrothokholi akho. Imbobo engemuva ye-USB-B eyokuxhuma irobhothi kukhompuyutha ephathekayo noma yedeskithophu, ukuze kusungulwe ukuxhumana nohlelo lokusebenza lwe-Opentrons olusebenza kukhompuyutha exhunyiwe. Imbobo ye-USB-A yangaphambili (USB-9), etholakala ngaphansi kwesibonisi sesikrini esithintwayo, inomsebenzi ofanayo nezimbobo ze-USB-A ezingemuva.
Qaphela: Izimbobo ze-USB zilinganiselwe amandla okuvikela irobhothi namadivayisi axhunyiwe. Ukulethwa kwamandla kuhlukaniswa ngaphakathi ngamaqembu amathathu embobo: izimbobo ze-USB-A ezingemuva kwesokunxele (i-USB-1 nge-USB-4), izimbobo ze-USB-A ezingemuva kwesokudla (i-USB-5 nge-USB-8), kanye ne-USB-A yangaphambili. ichweba. Iqembu ngalinye kulawa lizoletha ubukhulu obungu-500 mA kumadivayisi ahambisanayo e-USB 2.0.
Izixhumanisi zenethiwekhi
I-Opentrons Flex ingaxhuma kunethiwekhi yendawo ngoxhumano olunezintambo (Ethernet) noma olungenantambo (Wi-Fi).
Imbobo ye-Ethernet itholakala ngemuva kwerobhothi. Ixhume kuhabhu ye-Ethernet noma vula inethiwekhi yakho. Noma, kusukela kunguqulo yesistimu yerobhothi 7.1.0, ixhume ngqo embobeni ye-Ethernet ekhompyutheni yakho. Imojuli ye-Wi-Fi yangaphakathi isekela amanethiwekhi angu-802.11 ac/a/b/g/n anothi olukabili lwebhendi engu-2.4/5 GHz.

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ISAHLUKO 3: INCAZELO YOHLELO

3.6 Imininingwane yesistimu

Imininingwane ejwayelekile

Ubukhulu Isisindo Deck Slots
Isikrini sokuthinta
I-Wi-Fi Ethernet USB
Okokufaka kwamandla ekhamera yerobhothi
Izimpahla zinikezwa voltagUkuguquguquka kwe-e Imishini yokuhlinzeka ngokuguquguquka kwemvamisa Isistimu yokusabalalisa Isiphakeli sesekethe emfishane yamanje Ukwakheka kohlaka Ukwakheka kwewindi Izidingo zokungenisa umoya

87 × 69 × 84 cm / 34.25 × 27 × 33 ku-(W, D, H)
88.5 kg / 195 lb 12 ANSI/SLAS-izikhala ezihambisanayo endaweni yokusebenza
(kufinyeleleka kuma-pipette) 4 izikhala ezengeziwe ze-staging amathiphu kanye labware
(ifinyeleleka kuphela kuma-gripper) isikrini esithintwayo se-LCD esingu-7-intshi esinokrwabha kanye ne-Gorilla Glass 3 evikela ukulimala
802.11 ac/a/b/g/n dual-band (2.4/5 GHz)
100 Mbps 9 USB-A izimbobo 1 Imbobo ye-USB-B USB 2.0 isivinini
2MP, isithombe nevidiyo 100 VAC, 240 Hz, 50 60 A/1 VAC, 4.0 A/115 VAC
±10%
±5%
I-TN-S
6.3 A
Insimbi eqinile kanye ne-CNC aluminium design
Amafasitela aseceleni e-polycarbonate akhiphekayo nomnyango wangaphambili Okungenani u-20 cm / 8 phakathi kweyunithi nodonga

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ISAHLUKO 3: INCAZELO YOHLELO

Izidingo ze-PC exhunyiwe

Uhlelo lokusebenza lwe-Opentrons lusebenza ku-: Windows 10 noma kamuva i-macOS 10.10 noma eyakamuva Ubuntu 12.04 noma kamuva

Imininingwane yemvelo

Izimo zemvelo Izinga lokushisa elizungezile Umswakama ohlobene Idigri yokungcola

Ukusetshenziswa kwasendlini kuphela +20 kuya +25 °C (kunconyiwe) 40%, ukungajiki (kunconyiwe) 60 (ukungcola okungekhona okwenziwayo kuphela)

Ukuze uthole ulwazi olwengeziwe ngezimo zemvelo ezamukelekayo zokusetshenziswa nezokuthutha, bheka Imibandela Yezemvelo ingxenye yesahluko Ukufaka Nokuthuthwa.

Izitifiketi

Izitifiketi ziqediwe Akuqinisekisiwe/Akuqinisekisiwe

CE, ETL, FCC, ISO 9001 IVD, GMP

Isifinyezo solwazi lwesitifiketi siphrintwa esitikhani ngemuva kwe-Flex, eduze neswishi yokuvula/yokuvala. Ukuze uthole ulwazi oluningiliziwe lwesitifiketi nolwazi lokuthobela, bheka isigaba Sokuthobela Umthetho Esethulweni.

Inombolo yomkhiqizo
Yonke i-Flex inenombolo ye-serial ehlukile. Ifomethi yenombolo ye-serial inikeza ulwazi olwengeziwe, okuhlanganisa usuku lokukhiqiza irobhothi. Okwesiboneloample, inombolo ye-serial FLXA1020231007001 izokhombisa:

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ISAHLUKO 3: INCAZELO YOHLELO

Izinhlamvu FLX A10 2023 10 07 001

Iyunithi Yosuku Lwenyanga Yemodeli Yesigaba

Okusho ukuthi irobhothi i-Opentrons Flex. Ikhodi yenguqulo yokukhiqiza yerobhothi. Irobhothi lenziwe ngo-2023. Irobhothi lenziwe ngo-Okthoba. Irobhothi lenziwe ngosuku lwesi-7 lwenyanga. Inombolo ehlukile yamarobhothi enziwe ngosuku oluthile.

Ungathola inombolo ye-serial ye-Flex yakho:
Esitikhani sesitifiketi esingemuva kwe-Flex, eduze neswishi yokuvula/yokuvala. Ngakolunye uhlangothi lwesikrini esithintwayo (sibheke endaweni yokusebenza). Kuhlelo lokusebenza lwe-Opentrons ngaphansi kokuthi Amadivayisi > i-Flex yakho > Izilungiselelo zerobhothi > Okuthuthukisiwe.

I-OPENTRONS FLEX

ISAHLUKO 4
Amamojula
I-Opentrons Flex ihlanganisa nenani lamamojula wehadiwe we-Opentrons. Wonke amamojula angama-peripheral ahlala ezikhaleni zedekhi, futhi amaningi alawulwa irobhothi ngoxhumano lwe-USB.
Lesi sahluko sichaza imisebenzi kanye nokucaciswa ngokomzimba kwamamojula ahambisana nohlelo lwe-Opentrons Flex, kanye nendlela yokuwanamathisela nokuwalinganisa. Ukuze uthole imininingwane eyengeziwe mayelana nokusethwa kwemojuli nokusetshenziswa, bheka imanyuwali yamamojula ngamanye. Ukuze uthole imininingwane yokuhlanganisa amamojula kuzivumelwano zakho, bheka isigaba Somklami Wephrothokholi sesahluko Sokuthuthukiswa Kwephrothokholi noma imibhalo ye-Python Protocol API eku-inthanethi.
4.1 Amamojula asekelwe
I-Opentrons Flex ihambisana nezinhlobo ezine zamamojula e-Opentrons edekhini:
I-Heater-Shaker Module inikeza ukufudumeza kwedekhi kanye nokunyakaziswa kwe-orbital. Imojuli ingashisiswa ibe ngu-95 °C, futhi inganyakazisa sampngaphansi kuka-200 kuya ku-3000 rpm.
I-Magnetic Block iyidivayisi engenzi lutho ebambe ilebhu eduze nozibuthe bayo be-neodymium bamandla aphezulu. I-OT-2 Magnetic Module GEN1 ne-GEN2, ezihambisa omazibuthe bazo phezulu naphansi ngokuhlobene ne-labware, ayisekelwe ku-Opentrons Flex.
I-Temperature Module iyimojula yepuleti elishisayo nelibandayo elikwazi ukugcina izinga lokushisa elizinzile phakathi kuka-4 no-95 °C.
I-Thermocycler Module inikeza i-on-deck, i-thermocycling ezenzakalelayo ngokugcwele, evumela ukuzenzekelayo kwezinyathelo zokuhamba komsebenzi ezikhuphuka nomfula. I-Thermocycler GEN2 ihambisana ngokugcwele ne-gripper. I-Thermocycler GEN1 ayikwazi ukusetshenziswa ne-gripper, ngakho-ke ayisekelwe ku-Opentrons Flex.
Amanye amamojula aklanyelwe i-OT-2 ekuqaleni ayahambisana ne-Flex, njengoba kufingqiwe kuthebula elingezansi. Umaki wokuhlola ukhombisa ukuhambisana, futhi u-X ukhombisa ukungahambisani.

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ISAHLUKO 4: AMAMODULE

Uhlobo lwedivayisi nesizukulwane Imojuli ye-Heater-Shaker Module GEN1 Magnetic Module GEN1 Magnetic Module GEN2 Magnetic Block GEN1 Temperature Module GEN1 Temperature Module GEN2 Thermocycler Module GEN1 Thermocycler Module GEN2 HEPA Module

OT-2

I-Flex

4.2 Uhlelo lwe-module caddy
Amamojula ahambisanayo aklanyelwe ukungena kuma-caddies athatha isikhala ngaphansi kwedekhi. Lolu hlelo luvumela i-labware engaphezulu kwamamojula ukuthi ihlale iseduze nendawo yedekhi, futhi ivumela umzila wekhebula ongaphansi kwedekhi ukuze idekhi ihlale icocekile ngesikhathi iphrothokholi yakho isebenza.

Ama-Caddies we-Heater-Shaker, izinga lokushisa, kanye namamojula we-Thermocycler.

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ISAHLUKO 4: AMAMODULE
Ukuze ufake imojuli endaweni yedekhi, kufanele iqale ibekwe kumojula yemojuli ehambisanayo. Uhlobo ngalunye lwemojuli ehambisanayo lunomklamo walo we-caddy oqondanisa imojuli kanye nelebhu ngokunembile nedekhi ezungezile. (Okuhlukile i-Magnetic Block, engadingi amandla noma umzila wekhebula le-USB futhi ngaleyo ndlela ihlale ngokuqondile endaweni yedekhi.) Ama-Caddies amamojula athatha indawo eyodwa angabekwa noma yikuphi kukholamu 1 noma 3; I-Thermocycler ingafakwa kuphela ku-slots A1 ne-B1 kanyekanye.
Ngokuvamile, ukufaka i-module caddy:
1. Susa noma yiziphi izikhala zedekhi endaweni lapho kuzoya khona imojuli. 2. Beka imojuli ku-caddy yayo bese uqinisa amahange ayo. 3. Hambisa amandla emojula kanye nezintambo ze-USB kumakhava aseceleni, ukhuphuke ngembobo yedekhi engenalutho, futhi
zinamathisele kumojula. 4. Beka i-module caddy endaweni yokubeka bese uyikulungela endaweni yayo.
Ukuze uthole imiyalelo eqondile yokufaka, bheka I-Quickstart Guide noma Imanuwali Yemiyalo yemojula ethile. Ukuxhumeka kwekhebula kanye nendlela yokunamathisela ku-caddy kuyahlukahluka ngemojula.
4.3 Ukulinganiswa kwemojuli
Lapho uqala ukufaka imojuli ku-Flex, udinga ukusebenzisa ukulinganisa kokuma okuzenzakalelayo. Le nqubo ifana nokulinganisa indawo yamathuluzi, futhi iqinisekisa ukuthi i-Flex iya ezindaweni ezinembile ukuze kusebenze iphrothokholi efanele. Ngesikhathi sokulinganisa, i-Flex izothuthela ezindaweni nge-adaptha yokulinganisa yemojuli, ebukeka ifana nezikwele zokulinganisa eziyingxenye yezikhala zedekhi ezikhiphekayo.

Ama-adaptha okulinganisa ama-Heater-Shaker, izinga lokushisa, kanye namamojula we-Thermocycler.
Ukulinganisa amamojula kuyadingeka kuwo wonke amamojula afaka nge-caddy: I-Heater-Shaker, Ithempelesha, ne-Thermocycler Modules. I-Magnetic Block ayidingi ukulinganisa, futhi isilungele ukusetshenziswa ngokushesha nje lapho usibeka edekhini.

I-OPENTRONS FLEX

ISAHLUKO 4: AMAMODULE
Nini ukulinganisa amamojula
I-Flex ikutshela ngokuzenzakalelayo ukuthi ulinganise lapho uxhuma futhi uvula imojuli engenayo idatha yokulinganisa egciniwe. (Ungacashisa lesi saziso, kodwa ngeke ukwazi ukusebenzisa izivumelwano ngemojula uze uyilinganise.)
Uma usuqedile ukulinganisa, i-Flex igcina idatha yokulinganisa kanye nenombolo ye-serial yemojula ukuze isetshenziswe esikhathini esizayo. I-Flex ngeke ikwazise ukuthi ulungise kabusha ngaphandle uma ususa idatha yokulinganisa yaleyo mojula kuzilungiselelo zerobhothi. Ungakwazi ukuvula noma ukuvala imojuli yakho ngokukhululekile, noma uyihambise kwenye indawo yedekhi, ngaphandle kokudinga ukulinganisa kabusha. Uma ufuna ukulinganisa kabusha, ungaqala inqubo noma nini ekhadini lemojula Kuhlelo Lokusebenza lwe-Opentrons. (Ukulinganiswa kabusha akutholakali esikrinini esithintwayo.)
Ungalinganisa kanjani amamojula
Imiyalo ekusikrini esithintwayo noma kuhlelo lokusebenza lwe-Opentrons izokuqondisa ngenqubo yokulinganisa. Ngokuvamile izinyathelo yilezi:
1. Qoqa okokusebenza okudingekayo, okuhlanganisa i-adaptha yokulinganisa imojuli kanye ne-pipette calibration probe. 2. Beka i-adaptha yokulinganisa endaweni yemojuli futhi uqinisekise ukuthi isezingeni ngokuphelele.
Amanye amamojula angase adinge ukuthi ubophe i-adaptha kumojula. 3. Namathisela i-probe yokulinganisa ku-pipette. 4. I-Flex izohamba ngokuzenzakalelayo ukuze ithinte amaphuzu athile ku-adaptha yokulinganisa futhi iwalondoloze
amanani okulinganisa ukuze asetshenziswe esikhathini esizayo.
Uma ukulinganisa sekuqediwe futhi usuyikhiphile i-adaptha nophenyo, imojuli izobe isilungele ukusetshenziswa kumaphrothokholi.
Noma nini, ungakwazi view futhi uphathe idatha yakho yokulinganisa imojuli ku-Opentrons App. Iya kokuthi Izilungiselelo Zerobhothi ze-Flex yakho bese uchofoza ithebhu Yokulinganisa.

I-OPENTRONS FLEX

ISAHLUKO 4: AMAMODULE
4.4 I-Heater-Shaker Module GEN1

Izici ze-heater-Shaker
UKUSHISA NOKUDIKIZISA
I-Heater-Shaker inikeza ukufudumeza kwedekhi kanye nokunyakaziswa kwe-orbital. Imojula ingashiswa ifike ku-95 °C, nge-temperature pro elandelayofile:
Ibanga lokushisa: 37 °C Ukunemba kwezinga lokushisa: ±95 °C ku-0.5 °C Ukufana kwezinga lokushisa: ±55 °C ku-0.5 °CRamp izinga: 10 °C/min
Imojula inganyakazisa sampkancane ukusuka ku-200 kuye ku-3000 rpm, nalaba abalandelayo abanyakazayofile:
Ububanzi be-Orbital: 2.0 mm Ukuqondiswa kwe-Orbital: Ibanga lejubane elilandela iwashi: 200 rpm Ukunemba kwesivinini: ±3000 rpm
Imojula ine-latch yelebhu enikwe amandla yokuvikela amapuleti kumojuli ngaphambi kokuzamazama.

I-OPENTRONS FLEX

ISAHLUKO 4: AMAMODULE
AMA-ADAPTER OKUSHISA I-adaptha eshisayo ehambisanayo iyadingeka ukuze kwengezwe ilebula ku-Heater-Shaker. Ama-adaptha angathengwa ngokuqondile kwa-Opentrons ku-https://shop.opentrons.com. I-Thermal Adapter etholakalayo manje ihlanganisa:

I-adaptha ye-Universal Flat

I-adaptha ye-PCR

I-adaptha ye-Deep Well

I-adaptha ye-Flat Bottom

UKULAWULWA KWESOFITI
I-Heater-Shaker ihleleka ngokugcwele Ku-Protocol Designer kanye ne-Python Protocol API. I-Python API ivumela futhi ukuthi ezinye izinyathelo zephrothokholi zenziwe ngokuhambisana ngenkathi i-Heater-Shaker isebenza. Bona imiyalo engavimbeli emibhalweni ye-API ukuze uthole imininingwane yokwengeza izinyathelo ezihambisanayo kumaphrothokholi akho.
Ngaphandle kwamaphrothokholi, i-Opentrons App ingabonisa isimo samanje se-Heater-Shaker futhi ingalawula ngokuqondile i-heater, shaker, ne-latch yelebhu.

I-OPENTRONS FLEX

ISAHLUKO 4: AMAMODULE

Imininingwane ye-heater-Shaker

Ubukhulu Isisindo Imojula Okokufaka amandla I-adaptha yamandla Okokufaka kwe-adaptha enkulu I-voltage Fluctuation Overvoltage Ukusetshenziswa kwamandla

152 × 90 × 82 mm (L/W/H) 1.34 kg 36 VDC, 6.1 A 100 VAC, 240/50 Hz ±60% Isigaba II Ukungenzi lutho: 10 W

Okujwayelekile: Ukuzamazama: 4 W Ukushisisa: 11 W Ukushisisa nokuzamazama: 10 W

Izimo zemvelo Izinga lokushisa elizungezile Umswakama ohlobene ne-Altitude Digri yokungcola

Ubukhulu: 125 W Ukusetshenziswa kwasendlini kuphela 130 °C Kufika ku-20%, okungajiki Kufike ku-25 m ngaphezu kwezinga lolwandle 80

I-OPENTRONS FLEX

ISAHLUKO 4: AMAMODULE
4.5 I-Magnetic Block GEN1

Izici ze-Magnetic Block
I-Opentrons Magnetic Block GEN1 iyisibambi sepuleti esinozibuthe esingu-96. Amabhulokhi kazibuthe asetshenziswa kumaphrothokholi ancike kukazibuthe ukuze akhiphe izinhlayiya ekumisweni futhi azigcine ezitsheni zemithombo ngesikhathi sokugeza, ukuwasha, noma ezinye izinqubo zokukhishwa. Okwesiboneloample, ukulungiswa kwe-NGS okuzenzakalelayo; ukuhlanza i-DNA ye-genomic ne-mitochondrial, i-RNA, noma amaprotheni; kanye nezinye izinqubo zokukhipha zonke izimo zokusetshenziswa ezingabandakanya amabhlogo kazibuthe.
IZIMPENDULO ZAMAZWE
I-Magnetic Block ayinawo amandla, ayinazo izingxenye ze-elekthronikhi, futhi ayihambisani nobuhlalu kazibuthe phezulu noma phansi ngesixazululo. Imithombo iqukethe omazibuthe bendandatho be-neodymium abangu-96 abanamandla aphezulu agxiliswe embhedeni olayishwe intwasahlobo, osiza ukugcina ukubekezelelana phakathi kwebhulokhi nama-pipette ngenkathi kusetshenziswa amaphrothokholi azenzakalelayo.
UKULAWULWA KWESOFITI
I-Magnetic Block GEN1 ihleleka ngokugcwele Ku-Protocol Designer kanye ne-Python Protocol API.

I-OPENTRONS FLEX

ISAHLUKO 4: AMAMODULE

Ngaphandle kwamaphrothokholi, nokho, isikrini esithintwayo kanye nohlelo lokusebenza lwe-Opentrons azikwazi futhi azikwazi ukubonisa isimo samanje se-Magnetic Block GEN1. Lena imojula engenamandla. Ayiqukethe izinto zikagesi noma zemishini ezingaxhumana nerobhothi leFlex. “Ulawula” I-Magnetic Block usebenzisa izivumelwano ezisebenzisa i-Opentrons Flex Gripper ukuze wengeze futhi ukhiphe ilebula kule mojuli.

Imininingwane ye-Magnetic Block

Ubukhulu Isisindo Imojuli Amandla Ibanga likaMazibuthe Izimo zemvelo Amazinga okushisa asendaweni Umswakama ohlobene I-Altitude Digrii yokungcola

136 × 94 × 45 mm (L/W/H) 1.13 kg Ayikho, imojuli ayinawo amandla e-N52 neodymium Ukusetshenziswa kwangaphakathi kuphela okungu-20 °C 25%, akujikijeli Kufika ku-30 m ngaphezu kwezinga lolwandle 80

I-OPENTRONS FLEX

ISAHLUKO 4: AMAMODULE
4.6 I-Temperature Module GEN2

Izici zemojuli yezinga lokushisa
UKUSHISA NOKUPHOLA
I-Opentrons Temperature Module GEN2 iyimojuli yepuleti elishisayo nelibandayo. Ivamise ukusetshenziswa kumaphrothokholi adinga ukushisisa, ukupholisa, noma ukushintshwa kwezinga lokushisa. Imojula ingafinyelela futhi igcine amazinga okushisa asukela ku-4 °C kuya ku-95 °C kungakapheli imizuzu, kuye ngokumiswa kwemojuli nokuqukethwe.
AMABLOKI OKUSHISAYO
Ukuze ubambe i-labware ezingeni lokushisa, imojula isebenzisa amabhulokhi ashisayo e-aluminium. Imojula iza namabhulokhi angu-24well kanye namabhulokhi ashisayo angama-96. I-Temperature Module caddy iza nebhulokhi yomthombo ojulile kanye nebhulokhi ephansi eyisicaba eyenzelwe ukusetshenziswa ne-Flex Gripper. Amabhulokhi aphethe amashubhu angu-1.5 mL kanye no-2.0 mL, amapuleti e-PCR angu-96-well, imicu ye-PCR, amapuleti emithombo ejulile, namapuleti aphansi ayisicaba.

I-OPENTRONS FLEX

ISAHLUKO 4: AMAMODULE
Qaphela: Imojuli iphinde ithunyelwe ngebhulokhi ephansi eyisicaba ye-OT-2. Ungasebenzisi i-OT-2 block nge-Flex. Ibhulokhi ephansi eyisicaba ye-Flex inamagama athi “Opentrons Flex” phezulu. Le ye-OT-2 ayikwenzi.

24-umthombo we-thermal block

96-umthombo we-thermal block

Ibhulokhi eshisayo ejulile

Ibhulokhi eshisayo ephansi yeFlex

AMANZI OKUGEZA NOKUSHISA
Ngenxa yokuthi umoya uyisivikelo esihle sokushisa, izikhala phakathi kwe-labware kanye ne-thermal block zingathinta ukusebenza kwezinga lokushisa lesikhathi kweMojuli Yezinga lokushisa. Ukubeka amanzi amancane kumabhulokhi ashisayo angama-24- noma angama-96 kuqeda izikhala zomoya futhi kuthuthukisa ukusebenza kahle kokushisa. Inani elifanele lamanzi lincike ku-thermal block kanye ne-labware. Bheka Iphepha Elimhlophe Lemojuli Yezinga lokushisa ukuze uthole izincomo ezengeziwe.

UKULAWULWA KWESOFITI
I-Temperature Module ihleleka ngokugcwele Ku-Protocol Designer naku-Python Protocol API.
Ngaphandle kwamaphrothokholi, i-Opentrons App ingabonisa isimo samanje se-Temperature Module futhi ingalawula ngokuqondile izinga lokushisa le-surface plate.

I-OPENTRONS FLEX

ISAHLUKO 4: AMAMODULE

Izicaciso zemojuli yezinga lokushisa

Ubukhulu Isisindo Module amandla
Izimo zemvelo Izinga lokushisa elizungezile Umswakama ohlobene ne-Altitude Digri yokungcola

194 × 90 × 84 mm (L/W/H) 1.5 kg Okokufaka: 100 VAC, 240/50 Hz, 60 A Okukhiphayo: 4.0 VDC, 36 A, 6.1 W max Ukusetshenziswa kwangaphakathi kuphela <219.6 °C (kunconyelwa ukupholisa okuphelele ) Kufika ku-22%, okungancishisi Kufike ku-60 m ngaphezu kwezinga lolwandle 2000

4.7 Thermocycler Module GEN2

I-OPENTRONS FLEX

ISAHLUKO 4: AMAMODULE
Izici ze-Thermocycler
I-Opentrons Thermocycler Module GEN2 iyi-thermocycler esebenza ngokuzenzakalelayo esedekhini, ehlinzeka nge-PCR engenazandla ngefomethi yepuleti lemithombo engu-96. Isivalo saso esishisayo kanye nophawu olulahlwayo kufanelana ngokuqinile phezu kwepuleti, okuqinisekisa ukuthi i-s esebenza kahleampukufudumeza kanye nokuhwamuka okuncane.
UKUSHISA NOKUPHOLA Ibhulokhi ye-Thermocycler ingafudumeza futhi iphole, futhi isivalo sayo singashisisa, nge-temperature pro elandelayo.file: Ibanga lokushisa le-Thermal block: 4 °C Ibhulokhi eshisayo ephezulu yokushisa ramp izinga: 4.25 °C/s ukusuka ku-GEN2 kuya ku-95 °C Ibhulokhi eshisayo ephezulu yokupholisa ramp izinga: 2.0 °C/s ukusuka ku-95 °C kuya kububanzi bezinga lokushisa kwesivalo: 37 °C Ukunemba kwezinga lokushisa lesivalo: ±110 °C Isivalo esizenzakalelayo singavulwa noma sivalwe njengoba kudingeka ngesikhathi sokwenziwa kwephrothokholi.
THERMOCYCLER PROFILES I-Thermocycler ingakwazi ukusebenzisa i-profiles: ukuhamba ngebhayisikili ngokuzenzakalela ngokulandelana kwamazinga okushisa okuvimba ukuze wenze ukusabela okuzwela ukushisa.
I-RUBBER AUTOMATION SEALS I-Thermocycler iza nama-automation seals enjoloba ukusiza ukunciphisa ukuhwamuka. Uphawu ngalunye kufanele lufakwe inzalo ngaphambi kokusetshenziswa futhi lungasetshenziselwa imigijimo eminingana. Izigxivizo ezengeziwe zingathengwa ngokuqondile kwa-Opentrons ku-https://shop.opentrons.com.
UKULAWULWA KWE-SOFTWARE I-Thermocycler iyahleleka ngokugcwele Ku-Protocol Designer kanye ne-Python Protocol API. Ngaphandle kwamaphrothokholi, i-Opentrons App ingabonisa isimo samanje se-Thermocycler futhi ingalawula ngokuqondile izinga lokushisa lokuvimba, izinga lokushisa lesivalo, nendawo yesivalo.

I-OPENTRONS FLEX

ISAHLUKO 4: AMAMODULE

Imininingwane ye-Thermocycler

Ubukhulu (isivalo sivuliwe) Ubukhulu (isivalo sivaliwe) Isisindo (kuhlanganise nepayipi elingemuva) I-adaptha yamandla ivolthitage I-adaptha yamandla yamanje i-Overvoltage Izimo zemvelo Amazinga okushisa asendaweni Umswakama ohlobene ne-Altitude Izidingo zokungena komoya

244.95 × 172 × 310.1 mm (L/W/H) 244.95 × 172 × 170.35 mm (L/W/H) 8.4 kg 100 V ku-240/50 Hz 60 A Isigaba II Ukusetshenziswa kwangaphakathi 8.5 °C5 kuphela 20 °C (kuyamukeleka) 25%, akujikijeli Kufika ku-2 m ngaphezu kwezinga lolwandle Okungenani u-40 cm / 30 phakathi kweyunithi nodonga

I-OPENTRONS FLEX

ISAHLUKO 5
Ilebhu
Lesi sahluko sihlanganisa izinto eziku-Opentrons Labware Library ongazisebenzisa ne-Opentrons Flex kanye ne-Opentrons Flex Gripper. Iphinde ihlanganise ilebhu yangokwezifiso futhi, kubasebenzisi bethu bamandla, ixhuma izingxenye zelebhu ku-JSON yazo ehambisanayo. file izincazelo.
Ungathenga ilebula kubakhiqizi bokuqala bemishini noma esitolo se-Opentrons kokuthi https://shop.opentrons.com. Futhi, i-Opentrons ihlala isebenza ukuze iqinisekise izincazelo zelebhu entsha. Bona Ilabhulali Yelebhulali (exhunywe ngenhla) ukuze uthole uhlu lwakamuva.
5.1 Imiqondo yelebhu
I-Labware ihlanganisa okungaphezu nje kwezinto ezibekwe emphemeni futhi ezisetshenziswa kuphrothokholi. Ukukusiza uqonde ilebula ye-Opentrons, ake sihlole lesi sihloko ngemibono emithathu ehlukene. Ku-Opentrons Flex, ilebhu ihlanganisa izinto ezikuLabhulali yethu Yelebhu, idatha echaza ucezu ngalunye lwelebhu, kanye nelebhu yangokwezifiso.
Ilebhu njengehadiwe
I-Labware Library ihlanganisa yonke into ongayisebenzisa ngokuzenzakalelayo nge-Opentrons Flex. Lezi izingxenye ezihlala isikhathi eside kanye nezinto ezisebenzisekayo osebenza ngazo, ophinda uzisebenzisa, noma ozilahlayo ngenkathi usebenzisa iphrothokholi. Awudingi ukuthatha noma yiziphi izinyathelo ezikhethekile ukuze usebenze ngezinto ezikuLabhulali Yelebhu. Irobhothi leFlex liyakwazi ukusebenza ngayo yonke into esemtatsheni wezincwadi ngokuzenzakalelayo.
Ilebhu njengedatha
Ulwazi lwelebhu lugcinwe ku-Javascript object notation (JSON) files nge .json file izandiso. A JSON file ihlanganisa ubukhulu bendawo (ubude, ububanzi, ubude), umthamo wevolumu (L, mL), namanye amamethrikhi achaza izici ezingaphezulu, umumo wazo, nezindawo. Lapho isebenzisa umthetho olandelwayo, i-Flex ifunda lezi .json files ukwazi ukuthi iyiphi i-labware esedekhini kanye nendlela yokusebenza ngayo.

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE
Ilebhu yangokwezifiso
Ilebhu yangokwezifiso iyilebhu engafakiwe Kulabhulali Yelebhu noma iyilebhu edalwe Umdali Welebhu Ngokwezifiso. Kodwa-ke, kwesinye isikhathi umqondo welebhu yangokwezifiso ufika ucindezelwe yimibono yobunkimbinkimbi, izindleko, noma ubunzima. Kodwa, ilebhu yangokwezifiso akufanele ibe nzima ukuyiqonda noma ukuyidala. Ake sithathe isikhashana ukuqaqa umqondo welebhu yangokwezifiso.
Njengesiboneloampfuthi, I-Opentrons Labware Library ihlanganisa amapuleti anemithombo engama-96 (200 L) asuka e-Corning nase-BioRad, kodwa nabanye abakhiqizi benza lawa mapuleti emithombo. Futhi, ngenxa yezindinganiso zemboni ezivame ukwamukelwa, umehluko phakathi kwalezi zinto zelebhu ezitholakala yonke indawo mincane. Kodwa-ke, ipuleti elijwayelekile elingu-200 L, 96-well elivela ku-Stellar Scientific, Oxford Lab, noma i-Krackeler Scientific (noma yimuphi omunye umphakeli walolo daba) “iwuhlelo lwangokwezifiso” lwe-Flex ngoba aluchazwanga kusengaphambili Kulabhulali yethu Yelebhu. . Ukwengeza, umehluko omncane kubukhulu be-labware ungaba nomthelela omkhulu empumelelweni yokuqalisa kwephrothokholi yakho. Ngalesi sizathu, kubalulekile ukuba nencazelo enembile yelebhu yelebhu ngayinye ofuna ukuyisebenzisa kuphrothokholi yakho.
Futhi, nakuba i-labware yangokwezifiso ingaba ikhithi esoteric, ephuma kanye, isikhathi esiningi kuba nje amathiphu, amapuleti, amashubhu, namarack asetshenziswa nsuku zonke kumalebhu emhlabeni wonke. Futhi, umehluko kuphela phakathi kwelebhu ye-Opentrons kanye nelebhu yangokwezifiso into entsha ayichazwanga kusengaphambili kusofthiwe enika irobhothi amandla. I-Flex ingakwazi, futhi iyasebenza, nezinye izinto eziyisisekelo zelebhu noma okuthile okuhlukile, kodwa udinga ukurekhoda izici zaleyo nto encazelweni yelebhu ethi JSON. file bese ungenisa leyo datha ku-Opentrons App. Bona isigaba Sezincazelo Zelebhu Yangokwezifiso ngezansi ukuze uthole ulwazi olwengeziwe.
Ukufingqa, i-labware ihlanganisa:
Yonke into ekulabhulali ye-Opentrons Labware. Izincazelo zelebhu: idatha ku-JSON file echaza umumo, osayizi, namandla ezinto ngazinye
njengamapuleti emithombo, amathiphu, izindawo zokugcina amanzi, njll. Ilebhu yangokwezifiso, okuyizinto ezingafakiwe Kulabhulali Yelebhu.
Ngemva kabushaviewUma sibheka le mibono ebalulekile, ake sihlole izigaba nezinto ezikuLabhulali Yelebhu ye-Opentrons. Ngemva kwalokho, sizoqeda isahluko nge-overview yezingxenye zedatha yelebhu file futhi ufingqe izici namasevisi e-Opentrons akusiza ukuthi udale ilebhu yangokwezifiso.

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE

5.2 Amadamu
I-Opentrons Flex isebenza ngokuzenzakalelayo namachibi omthombo owodwa kanye namanzi amaningi abhalwe ngezansi. Ukusebenzisa lawa madamu kusiza ukwehlisa umthwalo wakho wokuzilungiselela ngoba avele alungele ukuzenzela ngaphandle kwebhokisi. Ulwazi lwe-reservoir luyatholakala naku-Opentrons Labware Library.

Amachibi omthombo owodwa

Imininingwane Yomkhiqizi

I-Agilent

290 mL V phansi

I-axygen

90 mL Iphansi eliphansi

I-NEST

195 mL Iphansi eliphansi

290 mL V phansi

Igama lokulayisha i-API
i-agilient_1_ reserve_290ml
i-axygen_1_ reserve_90ml
nest_1_ reserve_195ml
nest_1_ reserve_290ml

Amadamu anemithombo eminingi

Imininingwane Yomkhiqizi

I-NEST

Imithombo engu-12 15 mL/umthombo V phansi

I-USA Scientific

Imithombo engu-12 22 mL/umthombo V phansi

Igama lokulayisha i-API nest_12_ reservoir_15ml
usascientific_12_ reservoir_22ml

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE
Ama-Reservoirs kanye nezincazelo ze-API
I-Opentrons Labware Library ichaza izici zezindawo zokugcina amanzi ezibalwe ngenhla ku-JSON ehlukene files. Irobhothi kanye ne-Opentrons Python API zithembele kulezi zincazelo ze-JSON ukuze zisebenze ngelebhu esetshenziswa amaphrothokholi akho. Okwesiboneloample, lapho usebenza ne-API, umsebenzi we-ProtocolContext.load_labware wamukela lawa magama ngelebhu njengamapharamitha avumelekile kukhodi yakho. Amagama okulayisha e-API axhunyiwe axhumeka kuzincazelo zelebhu ye-reservoir endaweni ye-Opentrons GitHub.
Ilebhu yereservoir yangokwezifiso
Zama ukudala incazelo yelebhu yangokwezifiso nge-Opentrons Labware Creator uma idamu ongathanda ukulisebenzisa lingekho ohlwini lapha. Incazelo yangokwezifiso ihlanganisa bonke ubukhulu, imethadatha, umumo, umthamo wevolumu, nolunye ulwazi ku-JSON file. I-Opentrons Flex idinga lolu lwazi ukuze iqonde ukuthi usebenza kanjani ngelebhu yakho yangokwezifiso. Bheka isigaba Sezincazelo Zelebhu Yangokwezifiso ukuze uthole ulwazi olwengeziwe.
5.3 Amapuleti emithombo
I-Opentrons Flex isebenza ngokuzenzakalelayo ngamapuleti emithombo abhalwe ngezansi. Ukusebenzisa lawa ma-well plate kusiza ekwehliseni umthwalo wakho womsebenzi wokulungiselela ngoba avele azilungiselele ngaphandle kwebhokisi. Ulwazi lwepuleti le-Well luyatholakala naku-Opentrons Labware Library.

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE

6-imithombo amapuleti
Umkhiqizi Corning

Imininingwane
Imithombo engu-6 16.8 mL/umthombo Imithombo eyindilinga, phansi okuyisicaba

Igama lokulayisha i-API corning_6_wellplate_16.8ml_flat

12-imithombo amapuleti
Umkhiqizi Corning

Imininingwane
Imithombo engu-12 6.9 mL/umthombo Imithombo eyindilinga, phansi okuyisicaba

Igama lokulayisha i-API corning_12_wellplate_6.9ml_flat

24-imithombo amapuleti
Umkhiqizi Corning

Imininingwane
Imithombo engu-24 3.4 mL/umthombo Imithombo eyindilinga, phansi okuyisicaba

Igama lokulayisha i-API corning_24_wellplate_3.4ml_flat

48-imithombo amapuleti
Umkhiqizi Corning

Imininingwane
Imithombo engu-48 1.6 mL/umthombo Imithombo eyindilinga, phansi okuyisicaba

Igama lokulayisha i-API corning_48_wellplate_1.6ml_flat

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE

96-imithombo amapuleti
Umkhiqizi we-Bio-Rad Corning NEST
I-Opentrons Thermo Scientific
I-USA Scientific

Imininingwane
Imithombo engu-96 200 µL/umthombo Imithombo eyindilinga, V phansi
Imithombo engu-96 360 µL/umthombo Imithombo eyindilinga, phansi okuyisicaba
Imithombo engu-96 100 µL/umthombo Imithombo eyindilinga, V ngezansi isiketi esigcwele se-PCR
Imithombo engu-96 200 µL/umthombo Imithombo eyindilinga, phansi okuyisicaba
Imithombo ejulile engu-96 2000 µL/imithombo yesikwele, V phansi
Imithombo eqinile engu-96 200 µL/imithombo eyindilinga, V ngezansi PCR isiketi esigcwele
Imithombo ejulile ye-Nunc 96 1300 µL/imithombo eyindilinga, U phansi
Imithombo ejulile ye-Nunc 96 2000 µL/imithombo eyindilinga, U phansi
96 imithombo ejulile 2.4 mL/umthombo Square imithombo, U phansi

Igama lokulayisha i-API biorad_96_wellplate_200ul_pcr
corning_96_wellplate_360ul_flat
nest_96_wellplate_100ul_pcr_full_skirt
nest_96_wellplate_200ul_flat
nest_96_wellplate_2ml_deep
opentrons_96_wellplate_200ul_pcr_full_ isiketi
thermoscientificnunc_96_wellplate_ 1300ul thermoscientificnunc_96_wellplate_ 2000ul usascientific_96_wellplate_2.4ml_deep

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE

384-imithombo amapuleti

Umkhiqizi Osetshenziswa I-Biosystems Bio-Rad
Corning

Imininingwane
Imithombo engu-384 40 µL/umthombo Imithombo eyindilinga, V phansi
Imithombo engu-384 50 µL/umthombo Imithombo eyindilinga, V phansi
384 imithombo 112 µL/umthombo Square imithombo, phansi flat

Igama lokulayisha i-API appliedbiosystemsmicroamp_384_ wellplate_40ul biorad_384_wellplate_50ul
corning_384_wellplate_112ul_flat

Ama-adaptha amapuleti
Amapuleti e-aluminium abhalwe ngezansi angama-adaptha ashisayo emojuli ye-Opentrons Heater-Shaker GEN1. Ungasebenzisa lezi zincazelo ze-adaptha ezimele ukuze ulayishe ilebula ye-Opentrons eqinisekisiwe noma yangokwezifiso phezu kwe-Heater-Shaker.

I-adaptha yohlobo lwe-Opentrons Universal Flat Heater-Shaker Adapter Opentrons 96 PCR Heater-Shaker Adapter Opentrons 96 Deep Well Heater-Shaker Adapter Opentrons 96 Flat Bottom heater-Shaker Adapter

Igama lokulayisha i-API opentrons_universal_flat_adapter opentrons_96_pcr_adapter opentrons_96_deep_well_adapter opentrons_96_flat_bottom_adapter

Ungakwazi futhi ukulayisha kokubili i-adaptha nelebhu ngencazelo eyodwa. Ilabhulali yethu Yelebhu ihlanganisa i-adaptha eshisayo elungiselelwe ngaphambilini nezinhlanganisela zelabhu ezenza i-Heater-Shaker ilungele ukusetshenziswa ngaphandle kwebhokisi.

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE

Yazi: Ungasebenzisi incazelo ehlanganisiwe uma udinga ukuhambisa ilebula ungene noma uyisuse ku-Heater-Shaker phakathi nephrothokholi yakho, kungaba ngokubamba noma ngesandla. Sebenzisa incazelo ye-adaptha ezimele esikhundleni salokho.

Inhlanganisela ye-adaptha/ilebhu

Igama lokulayisha i-API

I-Opentrons 96 Deep Well Heater-Shaker Adapter ene-NEST Deep Well Plate 2 mL

opentrons_96_deep_well_adapter_nest_ wellplate_2ml_deep

I-Opentrons 96 Flat Bottom heater-Shaker Adapter ene-NEST 96 Well Plate 200 µL Flat

opentrons_96_flat_bottom_adapter_nest_ wellplate_200ul_flat

I-Opentrons 96 PCR Heater-Shaker Adapter ene-NEST Well Plate 100 µL

opentrons_96_pcr_adapter_nest_wellplate_ 100ul_pcr_full_skirt

I-Opentrons Universal Flat Heater-Shaker Adapter ene-Corning 384 Well Plate 112 µL Flat

opentrons_universal_flat_adapter_corning_384_ wellplate_112ul_flat

Ama-adaptha angathengwa ngokuqondile kwa-Opentrons ku-https://shop.opentrons.com.

Amapuleti omthombo kanye nezincazelo ze-API
I-Opentrons Labware Library ichaza izici zamapuleti emithombo abhalwe ngenhla ku-JSON ehlukene files. Irobhothi le-Flex kanye ne-Opentrons Python API ancike kulezi zincazelo ze-JSON ukuze asebenze ngelebhu esetshenziswa izivumelwano zakho. Okwesiboneloample, lapho usebenza ne-API, umsebenzi we-ProtocolContext.load_labware wamukela lawa magama ngelebhu njengamapharamitha avumelekile kukhodi yakho. Amagama okulayisha e-API axhunyiwe axhumeka ezincazelweni zelebhu yepuleti lomthombo endaweni yesikhombi se-Opentrons GitHub.

I-labware yamapuleti angokwezifiso
Zama ukusebenzisa i-Opentrons Labware Creator ukuze wenze incazelo yelebhu yangokwezifiso uma ipuleti lomthombo ongathanda ukulisebenzisa lingekho ohlwini lapha. Incazelo yangokwezifiso ihlanganisa bonke ubukhulu, imethadatha, umumo, umthamo wevolumu, nolunye ulwazi ku-JSON file. I-Opentrons Flex ifunda lolu lwazi ukuze iqonde ukuthi usebenza kanjani ngelebhu yakho yangokwezifiso. Bheka isigaba Sezincazelo Zelebhu Yangokwezifiso ukuze uthole ulwazi olwengeziwe.

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE

5.4 Amathiphu nama-tip racks
Amathiphu e-Opentrons Flex afika ngosayizi ongu-50 µL, 200 µL, no-1000 µL. Lawa amathiphu e-polypropylene acacile, angaphenduki atholakala ngezihlungi noma ngaphandle kwazo. Apakishwe ayinyumba kuma-rack aphethe amathiphu angu-96 futhi ayinayo i-DNase, i-RNase, i-protease, ama-pyrogens, i-DNA yomuntu, ama-endotoxin, nama-PCR inhibitors. Ama-Racks afaka nezinombolo eziningi kanye nezinsuku zokuphelelwa yisikhathi.
Amathiphu e-Flex pipette asebenza namapayipi e-Opentrons Flex 50 µL no-1000 µL ekucushweni kweshaneli engu-1, 8-, kanye nengu-96. Nakuba ungakwazi ukubeka noma yimaphi amathiphu e-Flex kumapayipi angu-50 L kanye no-1000 L, zama ukufanisa ithiphu nomthamo olinganiselwe we-pipette. Okwesiboneloample, kungase kube okungajwayelekile ukubeka ithiphu engu-1000 L ku-pipette engu-50 L. Ku-1000 L pipette, ungasebenzisa ithiphu engu-50 L, 200, noma i-1000 L.

Ama-racks amathiphu
Amathiphu angahlungiwe nahlungiwe anqwabelene ku-rack equkethe i-base plate esebenzisekayo kabusha, ipuleti elimaphakathi eliphethe amathiphu angu-96, nesivalo.

I-Tip rack ngevolumu 50 µL 200 µL 1000 µL

Igama lokulayisha i-API
Okungahlungiwe: opentrons_flex_96_tiprack_50ul Okuhlungiwe: opentrons_flex_96_filtertiprack_50ul
Okungahlungiwe: opentrons_flex_96_tiprack_200ul Okuhlungiwe: opentrons_flex_96_filtertiprack_200ul
Okungahlungiwe: opentrons_flex_96_tiprack_1000ul Okuhlungiwe: opentrons_flex_96_filtertiprack_1000ul

Ukusiza ngokuhlonza, i-tip rack mid-plates anekhodi yombala ngokusekelwe kusayizi wethiphu:
50 µL: i-magenta 200 L: ephuzi 1000 µL: eluhlaza okwesibhakabhaka

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE

Lapho u-oda noma u-oda kabusha, amathiphu nama-rack kuza ekucushweni okupakishwe okuhlukene okubili:
Ama-Racks: Aqukethe ama-tip racks ahlanganiswe ngokuhlukene (i-base plate, i-mid-plate enamathiphu, nesivalo). Ukulungiswa kwamaRakhi kuhamba phambili uma ukuhlanzeka kubalulekile, ukugwema ukungcoliswa, noma lapho izimiso zakho eziyisisekelo zingakuvumeli ukusetshenziswa kabusha kwe-base plate noma ingxenye.
Ukugcwalisa kabusha: Kuqukethe i-tip rack eyodwa (i-base plate, ipuleti eliphakathi elinamathiphu, nesivalo) kanye neziqukathi zamathiphu ngazinye. Ukucushwa kokugcwalisa kuhle kakhulu uma amaphrothokholi akho avumela ukusetshenziswa kabusha kwe-base plate noma ingxenye.
Ukuhambisana kwe-Tippipette
Amathiphu we-Flex pipette aklanyelwe amapayipi e-Opentrons Flex. Amathiphu e-Flex awahambisani emuva namapayipi e-Opentrons OT-2, futhi awukwazi ukusebenzisa amathiphu e-OT-2 kuma-Flex pipette.
Amanye amathiphu ajwayelekile embonini angasebenza nama-Flex pipette, kodwa lokhu akunconywa. Ukuze uqinisekise ukusebenza kahle, kufanele usebenzise amathiphu e-Opentrons Flex kuphela ngama-Flex pipette.
I-adaptha ye-rack yethiphu
I-pipette yeziteshi ezingu-96 idinga i-adaptha ukuze inamathisele i-rack egcwele yamathiphu ngendlela efanele. Ngesikhathi senqubo yokunamathisela, i-pipette ihamba phezu kwe-adaptha, izehlisele ezikhonkwaneni ezifakwayo, bese idonsa amathiphu kuma-pipette ngokuphakamisa i-adaptha kanye ne-tip rack.

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE

Qaphela: Sebenzisa kuphela i-adaptha ye-tip rack uma ucosha amathiphu agcwele ngesikhathi esisodwa. Beka ama-tip racks ngqo edekhini lapho ucosha amathiphu ambalwa.
Isexwayiso: Ncinza indawo engozini. Gcina izandla zikude ne-adaptha ye-tip rack ngenkathi i-pipette inamathisela amathiphu e-pipette.

I-adaptha yohlobo lwe-Opentrons Flex 96 I-adaptha ye-Tip Rack

Igama lokulayisha i-API opentrons_flex_96_tiprack_adapter

I-adaptha ye-tip rack iyahambisana ne-Opentrons Flex Gripper. Ungasebenzisa i-gripper ukubeka ama-tip racks amasha ku-adaptha noma ukucosha bese uhambisa ama-tip rack asetshenzisiwe ku-chute kadoti.

5.5 Amashubhu nama-tube racks

Uhlelo lwe-Opentrons 4-in-1 Tube Rack lusebenza ne-Opentrons Flex ngokuzenzakalelayo. Ukusebenzisa i-tube rack engu-4-in-1 kusiza ukunciphisa umthwalo wakho wokuzilungiselela ngoba izinhlanganisela ezihlinzekayo zilungele ukuzenzela ngaphandle kwebhokisi. Ulwazi olwengeziwe luyatholakala naku-Opentrons Labware Library.

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE
Inhlanganisela ye-tube ne-rack
I-Opentrons 4-in-1 tube rack isekela izinhlobonhlobo zamasayizi amashubhu, izinhlanganisela zobukhulu obuhlukile (ivolumu). Lokhu kufaka phakathi: i-6-tube rack yamashubhu angu-50 mL (6 x 50 mL). I-rack yenhlanganisela yamashubhu angu-10 yamashubhu amane angu-50 mL namashubhu ayisithupha angu-15 mL (4 x 50 mL, 6 x 15 mL). I-15-tube rack yamashubhu angu-15 mL (15 x 15 mL). 24-tube rack for 0.5 mL, 1.5 mL, noma 2 mL amashubhu (24 x 0.5 mL, 1.5 mL, 2 mL).
Qaphela: Wonke amashubhu ayisilinda anezansi ezimise okuka-V (ikhonikhi) ngaphandle uma kuboniswe ngenye indlela.

6-tube racks
Uhlobo lweshubhu 6 Falcon 50 mL 6 NEST 50 mL
10-tube racks
Uhlobo lweshubhu 4 Falcon 50 mL 6 Falcon 15 mL 4 NEST 50 mL 6 NEST 15 mL

Igama lokulayisha i-API opentrons_6_tuberack_falcon_50ml_conical opentrons_6_tuberack_nest_50ml_conical
API load name opentrons_10_tuberack_falcon_4x50ml_6x15ml_conical opentrons_10_tuberack_nest_4x50ml_6x15ml_conical

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE

15-tube racks
Uhlobo lweshubhu 15 Falcon 15 mL 15 NEST 15 mL

Igama lokulayisha i-API opentrons_15_tuberack_falcon_15ml_conical opentrons_15_tuberack_nest_15ml_conical

24-tube racks

Uhlobo lwe-Tube
24 I-Eppendorf Safe-Lock 1.5 mL 24 Eppendorf Safe-Lock 2 mL, phansi okumise okuka-U 24 okuvamile 2 mL isikulufu cap 24 NEST 0.5 mL isikulufu cap 24 NEST 1.5 mL isikulufu cap 24 NEST 1.5 mL snap 24 m L2 cap 24 m I-NEST 2 mL snap cap, ezansi ezimise oku-U

Igama lokulayisha i-API opentrons_24_tuberack_eppendorf_1.5ml_safelock_snapcap opentrons_24_tuberack_eppendorf_2ml_safelock_snapcap
opentrons_24_tuberack_generic_2ml_screwcap opentrons_24_tuberack_nest_0.5ml_screwcap opentrons_24_tuberack_nest_1.5ml_screwcap opentrons_24_tuberack_nest_1.5ml_snapcap opentrons_24_tuberack_nest_2ml_screwcap opentrons_24_tuberack_nest_2ml_snapcap

Izincazelo ze-Tube rack API
I-Opentrons Labware Library ichaza izici zama-tube racks abhalwe ngenhla ku-JSON ehlukene files. Irobhothi le-Flex kanye ne-Opentrons Python API ancike kulezi zincazelo ze-JSON ukuze asebenze ngelebhu esetshenziswa izivumelwano zakho. Okwesiboneloample, lapho usebenza ne-API, umsebenzi we-ProtocolContext.load_labware wamukela lawa magama ngelebhu njengamapharamitha avumelekile kukhodi yakho. Amagama okulayisha e-API axhunyiwe axhumeka kuzincazelo zelebhu ye-tube rack ku-Opentrons GitHub repository.

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE
I-labware ye-tube rack yangokwezifiso
Zama ukudala incazelo yelebhu yangokwezifiso usebenzisa i-Opentrons Labware Creator uma ishubhu nenhlanganisela yokubeka ongathanda ukuyisebenzisa ingekho ohlwini lapha. Incazelo yangokwezifiso ihlanganisa bonke ubukhulu, imethadatha, umumo, umthamo wevolumu, nolunye ulwazi ku-JSON file. I-Opentrons Flex ifunda lolu lwazi ukuze iqonde ukuthi usebenza kanjani ngelebhu yakho yangokwezifiso. Bheka isigaba Sezincazelo Zelebhu Yangokwezifiso ukuze uthole ulwazi olwengeziwe.
5.6 Amabhulokhi e-Aluminium
I-Aluminium ivimba umkhumbi nge-Temperature Module GEN2 futhi ingathengwa ngokuhlukana njengesethi eneziqephu ezintathu. Isethi ihlanganisa ipuleti eliphansi eliyisicaba, ibhulokhi elinemithombo engu-24, kanye nebhulokhi elinemithombo engu-96.
I-Opentrons Flex isebenzisa amabhulokhi e-aluminium ukuze ibambe u-sampamashubhu kanye namapuleti emithombo ku-Temperature Module noma ngqo emphemeni. Uma isetshenziswa ne-Temperature Module, amabhlogo e-aluminium angagcina i-sampamashubhu, imicu ye-PCR, noma amapuleti ezingeni lokushisa elingashintshi phakathi kuka-4 °C no-95 °C.
Ipuleti eliphansi eliphansi
Ipuleti eliphansi eliyisicaba lemikhumbi ye-Flex eline-caddy ye-Temperature Module futhi lihambisana namapuleti emithombo ajwayelekile e-ANSI/SLAS. Leli puleti eliyisicaba lihlukile kupuleti elithunyelwa nge-Temperature Module ngokwayo noma isethi eneziqephu ezintathu ezihlukene. Ifaka indawo yokusebenza ebanzi kanye neziqeshana zamakhona ezinama-chamfered. Lezi zici zisiza ukuthuthukisa ukusebenza kwe-Opentrons Flex Gripper lapho ihambisa ilebula kupuleti noma liyikhipha.
Ungakwazi ukusho ukuthi iliphi ipuleti eliphansi eliyisicaba onalo ngoba leli le-Flex linamagama athi “Opentrons Flex” endaweni yalo engaphezulu. Le ye-OT-2 ayikwenzi.

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE

Ibhulokhi ye-aluminium engama-24
Ibhulokhi enemithombo engama-24 isetshenziswa neziqephu zomuntu ngamunyeample izitsha. Okwesiboneloample, yemukela sample vials ukuthi:
Yiba nezansi ezimise okwe-V noma ezimise oku-U. Vikela okuqukethwe nge-snap cap noma ngokuvalwa kwe-screw cap. Bamba uketshezi ngamanani angu-0.5 mL, 1.5 mL, no-2 mL.

Ibhulokhi ye-aluminium engama-96
Ibhulokhi enemithombo engu-96 isekela izinhlobo eziningi zamapuleti omthombo. Okwesiboneloample, yamukela kahle amapuleti athi:
Kusuka kubakhiqizi abakhulu bamapuleti afana ne-Bio-Rad ne-NEST.
Idizayinelwe ngezingezansi ezimise okuka-V, ezansi ezimise oku-U, noma ezansi eziyisicaba.
Idizayinelwe ngemithombo engu-100 µL noma engu-200 µL.
Iphinde ihambisane nemicu ye-PCR ejwayelekile.

Ama-adaptha azimele
Ibhulokhi eshisayo i-Flex flat floor plate 24-well aluminium block 96-well aluminium block

Igama lokulayisha le-API i-opentrons_aluminum_flat_bottom_plate Bona inhlanganisela yelebhu ngezansi. opentrons_96_well_aluminium_block

Inhlanganisela yelebhu ye-Aluminium block
I-Opentrons Labware Library isekela inhlanganisela elandelayo yebhulokhi, ibhodlela, nepuleti lemithombo, nayo echazwe ngencazelo ehlukene yelebhu ye-JSON. files. Irobhothi leFlex kanye ne-Opentrons Python API

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE
thembela kulezi zincazelo ze-JSON ukuze usebenze ngelebhu esetshenziswa amaphrothokholi akho. Okwesiboneloample, lapho usebenza ne-API, umsebenzi we-ProtocolContext.load_labware wamukela lawa magama ngelebhu njengamapharamitha avumelekile kukhodi yakho. Amathebula angezansi aklelisa izinhlanganisela ezizenzakalelayo zebhulokhi/isitsha kanye namagama omthwalo we-API ahlobene. Izixhumanisi zixhuma ezincazelweni ezihambisanayo ze-JSON endaweni yekhosombe ye-Opentrons GitHub.
Qaphela: Wonke amashubhu anezansi ezimise okuka-V ngaphandle uma kuboniswe ngenye indlela.

Inhlanganisela yelebhu yebhulokhi ye-aluminium engama-24

Okuqukethwe kwebhulokhi engu-24 Okujwayelekile 2 mL isikulufu cap NEST 0.5 mL isikulufu cap NEST 1.5 mL isikulufu cap NEST 1.5 mL snap cap NEST 2 mL isikulufu cap NEST 2 mL snap cap, ngezansi okumise oku-U

Igama lokulayisha i-API i-opentrons_24_aluminumblock_generic_2ml_screwcap opentrons_24_aluminumblock_nest_0.5ml_screwcap opentrons_24_aluminumblock_nest_1.5ml_screwcap opentrons_24_aluminumblock_nest_1.5ml ns_24_aluminiumblock_nest_2ml_snapcap

Inhlanganisela yelebhu yebhulokhi ye-aluminium engama-96

Okuqukethwe kwebhulokhi engu-96 ye-Bio-Rad well plate 200 µL Isiqeshana se-PCR esijwayelekile esingu-200 µL NEST ipuleti lomthombo 100 µL

Igama lokulayisha i-API opentrons_96_aluminumblock_biorad_wellplate_200uL opentrons_96_aluminumblock_generic_pcr_strip_200uL opentrons_96_aluminumblock_nest_wellplate_100uL

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE

5.7 I-Labware kanye ne-Opentrons Flex Gripper
Nakuba i-Opentrons Flex isebenza nakho konke okuku-Labware Library, i-Opentrons Flex Gripper ihambisana nezinto ezithile zelebhu kuphela. Njengamanje, i-Gripper ilungiselelwe ukusetshenziswa nezinto ezilandelayo zelebhu.

Isigaba seLabhulali Amapuleti Ajulile Agxunyekwe Ngokugcwele Amapuleti angama-96
Ama-Tip Racks (amathiphu angahlungiwe nahlungiwe)

Amabhrendi
I-NEST 96 I-Deep Well Plate 2 mL
I-Opentrons Tough 96 Well Plate 200 µL PCR Full Skirt NEST 96 Well Plate 200 µL Flat
I-Opentrons Flex 96 Tip Rack 50 µL Opentrons Flex 96 Tip Rack 200 µL Opentrons Flex 96 Tip Rack 1000 µL

Qaphela: Ukuze uthole imiphumela engcono kakhulu, sebenzisa i-Flex Gripper kuphela ilebhu ebhalwe ngenhla. I-Flex Gripper ingase isebenze nenye ilebhu ethobelayo ye-ANSI/SLAS ezenzakalelayo, kodwa lokhu akunconywa.

5.8 Izincazelo zelebhu yangokwezifiso
Njengoba kuxoxiwe ekuqaleni kwalesi sahluko, i-Labware yangokwezifiso i-labware engekho ohlwini lwe-Opentrons Labware Library. Ungasebenzisa ezinye izinto ezijwayelekile noma ezihlukile zelebhu nge-Flex ngokulinganisa ngokunembile futhi urekhode izici zaleyo nto futhi ulondoloze leyo datha ku-JSON. file. Uma kungeniswa kuhlelo lokusebenza, i-Flex ne-API isebenzisa leyo datha ye-JSON ukuze ixhumane nelebhu yakho. I-Opentrons inikeza amathuluzi namasevisi, esizowahlola ngezansi, ukuze akusize usebenzise i-Flex ngelebhu yangokwezifiso.

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE

Ukudala izincazelo zelebhu yangokwezifiso
Amathuluzi nezinsizakalo ze-Opentrons zisiza ukubeka ilebhu yangokwezifiso endaweni ongafinyelela kuyo. Lezi zici zivumela amazinga ahlukene wamakhono nezindlela zokusebenza. Ukwakha eyakho i-labware, futhi uyisebenzise ne-Opentrons Flex, kusiza ukwenza irobhothi libe ukungezwani okuguquguqukayo nokunamandla elebhu yakho.

UMDALI WE-LABWARE YANGEMPELA
Umdali Welebhu Yangokwezifiso awunakhodi, web-ithuluzi elisekelwe elisebenzisa i-graphical interface ukukusiza ukuthi udale incazelo yelebhu file. Umdali welebhu ukhiqiza incazelo yelebhu ye-JSON file oyingenisa ku-Opentrons App. Ngemuva kwalokho, ilebhu yakho yangokwezifiso iyatholakala kurobhothi leFlex kanye nePython API.

INKONZO YE-LABWARE YANGEMPELA
Thintana nathi uma i-labware ongathanda ukuyisebenzisa ingatholakali kulabhulali, uma ungakwazi ukudala izincazelo zakho, noma ngenxa yokuthi into yangokwezifiso ihlanganisa izimo ezihlukene, osayizi, noma okunye okungahambi kahle okuchazwe ngezansi.

Ilebhu ongayichaza kuMdali Welebhu
; Imithombo namashubhu kufana futhi kuyafana. ; Yonke imigqa ihlukaniswe ngokulinganayo
(isikhala phakathi kwemigqa siyalingana).
; Wonke amakholomu ahlukaniswe ngokulinganayo (isikhala phakathi kwamakholomu siyalingana).
; Ilingana kahle endaweni eyodwa yedekhi.

I-Labware Opentrons idinga ukuchaza; Imithombo kanye nokuma kweshubhu kuyahlukahluka. ; Imigqa ayihlukanisiwe ngokulinganayo.
; Amakholomu awahlukanisiwe ngokulinganayo.
; Incane kunesikhala sedekhi eyodwa (idinga i-adaptha) noma ihlanganisa izikhala eziningi zedekhi.

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE
Nansi eminye imidwebo ekusiza ukuthi ubone ngeso lengqondo i-exampokuchazwe ngenhla. Okuvamile Wonke amakholomu ahlukaniswe ngokulinganayo futhi yonke imigqa ihlukaniswe ngokulinganayo. Amakholomu awadingi ukuba nesikhala esifanayo nemigqa.
Okuvamile Igridi akudingeki ibe maphakathi ne-labware.
Imigqa Engajwayelekile ihlukaniswe ngokulinganayo kodwa amakholomu awahlukani ngokulinganayo.
Amakholomu/imigqa Engajwayelekile ihlukaniswe ngokulinganayo kodwa imithombo ayifani.
Okungajwayelekile Kunegridi engaphezu kweyodwa.
Ithimba lethu lelebhu lizosebenza ukuze liqonde izidingo zakho futhi likuklamele izincazelo zelebhu yangokwezifiso. Bona izindatshana zosekelo Ezicela Incazelo Yelebhu Yangokwezifiso kanye nefomu lesicelo selebhu yangokwezifiso ukuze uthole ulwazi olwengeziwe. Lena isevisi esekelwe enkokhelweni.

I-OPENTRONS FLEX

ISAHLUKO 5: I-LABWARE

PYTHON API
Nakuba ungeke ukwazi ukudala ilebula yangokwezifiso nge-API yethu, ungasebenzisa ilebhu yangokwezifiso nezindlela ze-API ezitholakalayo. Nokho, udinga ukuchaza ilebhu yakho yangokwezifiso kuqala bese uyingenisa ku-Opentrons App. Uma usungeze ilebula yakho kuhlelo lokusebenza lwe-Opentrons, iyatholakala ku-Python API kanye nerobhothi. Bona isigaba Sezincazelo Zelebhu Yangokwezifiso semibhalo ye-Python API ukuze uthole ulwazi olwengeziwe. Ukuze uthole ulwazi mayelana nokubhala izikripthi zephrothokholi nge-API, bona isigaba se-Python Protocol API kusahluko Sokuthuthukiswa Kwephrothokholi.
I-schema yelebhu ye-JSON
A JSON file ipulani ye-Opentrons ejwayelekile kanye nelebhu yangokwezifiso. Lokhu file iqukethe futhi ihlele idatha yelebhu ngokwezicaciso zedizayini ezisethwe i-schema ezenzakalelayo.
I-schema wuhlaka

Amadokhumenti / Izinsiza

opentrons FLEX FLEX Opentrons Flex Open Source Liquid Handling Robot [pdf] Incwadi Yeziqondiso
I-FLEX Opentrons Flex I-Open Source Liquid Handling Robot, i-FLEX, i-Opentrons Flex Open Source Liquid Handling Robot, i-Flex Open Source Liquid Handling Robot, I-Open Source Liquid Handling Robot, Irobhothi Lokuphatha Uketshezi Lomthombo, Irobhothi Lokuphatha Uketshezi, Irobhothi Lokuphatha, Irobhothi

Izithenjwa

Imaniwali yosebenzisayo

FLEX Opentrons Flex Open Source Liquid Handling Robot

I-Opentrons Flex

Imininingwane:

Imiyalo yokusetshenziswa komkhiqizo:

1. Ukufakwa nokulinganiswa kwezinsimbi:

2. Ukuthutha:

3. Izixhumanisi:

4. Umklami Wephrothokholi:

5. I-Python Protocol API:

6. Amaphrothokholi we-OT-2:

Imibuzo Evame Ukubuzwa (FAQ):

Q: Ngiyixazulula kanjani inkinga uma irobhothi lingahambi njenge
kulindeleke?

Q: Ngingasebenzisa ama-pipettes angokwezifiso nge-Opentrons Flex?

Amadokhumenti / Izinsiza

Izithenjwa

Shiya amazwana

Khansela impendulo

FLEX Opentrons Flex Open Source Liquid Handling Robot

I-Opentrons Flex

Imininingwane:

Imiyalo yokusetshenziswa komkhiqizo:

1. Ukufakwa nokulinganiswa kwezinsimbi:

2. Ukuthutha:

3. Izixhumanisi:

4. Umklami Wephrothokholi:

5. I-Python Protocol API:

6. Amaphrothokholi we-OT-2:

Imibuzo Evame Ukubuzwa (FAQ):

Q: Ngiyixazulula kanjani inkinga uma irobhothi lingahambi njenge kulindeleke?

Q: Ngingasebenzisa ama-pipettes angokwezifiso nge-Opentrons Flex?

Amadokhumenti / Izinsiza

Izithenjwa

Okuthunyelwe Okuhlobene

Shiya amazwana

Khansela impendulo

Q: Ngiyixazulula kanjani inkinga uma irobhothi lingahambi njenge
kulindeleke?