Iingcaciso ngokubanzi: Lorem ipsum dolor sit
amet, consectetur adipiscing elit. Sed do eiusmod tempor incididunt
ut labore et dolore magna aliqua.
Iinkcukacha zokusingqongileyo: Lorem ipsum
dolor sit amet, consectetur adipiscing elit. Sed ukwenza eiusmod tempor
incididunt ut labore et dolore magna aliqua.

Iziqinisekiso: Lorem ipsum dolor sit amet,
consectetur adipiscing elit. Sed do eiusmod tempor incididunt ut
labore et dolore magna aliqua.
Inombolo yesiriyeli: XXX-XXXX-XXXX

Imiyalelo yokusetyenziswa kwemveliso:

1. UFakelo lwesixhobo kunye noLungiso:

Landela amanyathelo achazwe kwincwadana ye-pipette kunye ne-gripper
ukufakela.

2. Ukufuduswa:

Xa ufuna iintshukumo ezimfutshane, jonga kwicandelo 2.5 kwincwadana yemigaqo. Kuba
imigama emide, landela izikhokelo ezinikiweyo. Ukuhamba ngokubanzi
iingcebiso ziyafumaneka.

3. Uqhagamshelwano:

Qinisekisa uqhagamshelo lwamandla olululo njengoko kuchaziwe kwincwadana.
Qhagamshela i-USB kunye nezixhobo ezincedisayo njengoko kufuneka. Uqhagamshelwano lwenethiwekhi
kufuneka zisekwe ngokulandela imiyalelo.

4. Umyili weProtocol:

Qonda iimfuno zoMyili weProtocol kwaye ufunde ukuba njani
ukuyila iiprothokholi ezintsha okanye ukuguqula esele zikhona ngokwelabhu yakho
iimfuno.

5. IPython Protocol API:

Phonononga ukubhala kunye nokuqhuba izikripthi usebenzisa iPython Protocol API.
Fumanisa iimpawu ezikhethekileyo zePython zokuphucula ukusebenza.

6. IiProthokholi ze-OT-2:

Funda malunga neeprothokholi ze-OT-2 zePython, iiprothokholi ze-OT-2 JSON, kunye
Iiprothokholi zeModyuli yeMagnetic yeentlobo ezahlukeneyo zovavanyo.

Imibuzo Ebuzwa Rhoqo (FAQ):

Umbuzo: Ndiyicombulula njani ingxaki ukuba irobhothi ayihambi njenge
kulindeleke?

A: Jonga uqhagamshelo lwamandla, uqinisekise ulungelelwaniso olululo lwe
izixhobo, kwaye uqinisekise ukuba akukho zithintelo kwi
indlela yerobhothi.

Umbuzo: Ndingasebenzisa iipipettes zesiko kunye ne-Opentrons Flex?

A: I-Opentrons icebisa ukuba kusetyenziswe iipipette ezihambelanayo ngokugqibeleleyo
ukusebenza kunye nokuchaneka.

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I-Opentrons Flex
Incwadi Yemiyalelo
I-Opentrons Labworks Inc.
Disemba 2023

© OPENTRONS 2023 Opentrons FlexTM (Opentrons Labworks, Inc.) Amagama abhalisiweyo, iimpawu zorhwebo, njl.njl. ezisetyenziswe kolu xwebhu, naxa zingaphawulwanga ngokuthe ngqo ngolo hlobo, azinakuqwalaselwa njengezingakhuselwanga ngumthetho.

Isiqulatho
Intshayelelo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 Isakhiwo sale ncwadana . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Amanqaku nezilumkiso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Isahluko 1: Intshayelelo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 1.1 Wamkelekile kwi-Opentrons Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Yintoni entsha kwiFlex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Flex workstations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 1.2 Ulwazi ngokhuseleko . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Iimpawu zokhuseleko. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Izilumkiso zokhuseleko lombane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Izilumkiso ezongezelelweyo zokhuseleko . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 Izilumkiso zokhuseleko . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 Ukhuseleko lwebhayoloji . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Umsi onetyhefu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Ulwelo olunokutsha . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 1.3 Ukuthotyelwa kwemigaqo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Ukhuseleko . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Ukuhambelana koMbane . . . . . . . . . . . . . . . . . . . . . . . . .20 Izilumkiso kunye namanqaku eFCC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Ukuthotyelwa kwe-ISED yaseKhanada . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Isilumkiso sokusingqongileyo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Wi-Fi yangaphambili. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Isahluko 2: Ukufakela kunye nokuTshintshwa. . . . . . . . . . . . . . . . . . . . . . . 22 2.1 Iimfuno zokhuseleko kunye nokusebenza. . . . . . . . . . . . . . . . . . . . .22 Ibekwe phi iOpentrons Flex . . . . . . . . . . . . . . . . . . . . . . . .22 Ukusetyenziswa kwamandla . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 Iimeko zokusingqongileyo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 2.2 Unboxing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Umgudu kunye nexesha elifunekayo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Ikhreyithi kunye nezinto zokupakisha . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Izinto zemveliso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 Icandelo 1: Susa ikhreyithi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27 Icandelo 2: Khupha iFlex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29 Icandelo 3: Indibano yokugqibela namandla . . . . . . . . . . . . . . . . . . .32

2.3 Ukubaleka okokuqala . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Vula amandla. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Qhagamshela kwinethiwekhi okanye ikhompyutha . . . . . . . . . . . . . . . . . . . . .36 Faka uhlaziyo lwesoftware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37 Qhoboshela i-Emergency Stop Pendant . . . . . . . . . . . . . . . . . . . . . . 38 Nika irobhothi yakho igama. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
2.4 Ufakelo lwesixhobo kunye nolungelelwaniso . . . . . . . . . . . . . . . . . 38 Ukufakwa kwePipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 Ufakelo lwegripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
2.5 Ukufuduswa kwenye indawo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 Iintshukumo ezimfutshane. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Iintshukumo ezinde . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Isiluleko esichukumisayo ngokubanzi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 Iingcinga zokugqibela malunga nokuhamba. . . . . . . . . . . . . . . . . . . . . . . . . . .44
Isahluko 3: Inkcazo yeNkqubo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 3.1 Amalungu omzimba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45 Isakhelo kunye nendawo ebiyelweyo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 Idekhi kunye nendawo yokusebenza . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 Stagindawo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47 Ukulungiswa kwedekhi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 I-chute yenkunkuma. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49 Stagiindawo zokubeka indawo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49 Inkqubo yentshukumo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 Isikrini esichukumisayo kunye neziboniso ze-LED . . . . . . . . . . . . . . . . . . . . . . . . .51 3.2 IiPipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 Pipette iinkcukacha. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Ukulinganisa kwePipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 iadaptha ye-rack ye-Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Ukuthatha ingcebiso ngokuyinxenye . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 Pipette abenzi boluvo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 Pipette uhlaziyo lwefirmware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 3.3 Igripper. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 Iinkcukacha zokubala . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Gripper ukulinganisa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Gripper uhlaziyo lwe-firmware. . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 3.4 I-Emergency Stop Pendant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 Isetyenziswa nini i-E-stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 Ukuzibandakanya kunye nokukhulula i-E-stop . . . . . . . . . . . . . . . . . . . . .60

3.5 Uqhagamshelwano . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 Uqhagamshelo lwamandla . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 USB kunye noqhagamshelwano oluncedisayo. . . . . . . . . . . . . . . . . . . . . . . . .62 Uqhagamshelwano lwenethiwekhi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
3.6 Iinkcukacha zenkqubo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63 Iinkcukacha ngokubanzi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63 Iinkcukacha zokusingqongileyo . . . . . . . . . . . . . . . . . . . . . . . . . .64 Iziqinisekiso. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 Inombolo yothotho . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64
Isahluko 4: Iimodyuli. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 4.1 Iimodyuli ezixhaswayo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66 4.2 Inkqubo yemodyuli yecaddy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 4.3 Ulungelelwaniso lwemodyuli . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68 Ulungelelaniswa nini iimodyuli. . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 Ulungelelaniswa njani na iimodyuli . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 4.4 Imodyuli ye-Heater-Shaker GEN1 . . . . . . . . . . . . . . . . . . . . . . . . . .70 Iimpawu ze-Heater-Shaker. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70 Iinkcukacha ze-Heater-Shaker . . . . . . . . . . . . . . . . . . . . . . . . . .72 4.5 Ibhloko yeMagnetic GEN1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 Iimpawu zeBlock yeMagnetic. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 Iimpawu zeBhloko yeMagnetic . . . . . . . . . . . . . . . . . . . . . . . . .74 4.6 Imodyuli yobushushu GEN2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .75 Iimpawu zeModyuli yoBuqondo . . . . . . . . . . . . . . . . . . . . . . . . .75 Imilinganiselo yeModyuli yoBuqondo . . . . . . . . . . . . . . . . . . . .77 4.7 Imodyuli yeThermocycler GEN2. . . . . . . . . . . . . . . . . . . . . . . . . . . .77 Iimpawu zeThermocycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .78 Iimpawu zeThermocycler . . . . . . . . . . . . . . . . . . . . . . . . . . .79
Isahluko 5: ILebhu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 5.1 Iikhonsepthi zeLebhu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 ILebhu njengehardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 ILebhu njengedatha . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Ilebhu yesiko . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81 5.2 Amadama . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Amadama anequla elinye . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Amadama amaqula amaninzi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Amadama kunye neenkcazelo zeAPI . . . . . . . . . . . . . . . . . . . . . . . . 83 Ilebhu yereservoir eqhelekileyo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 5.3 Iipleyiti zamaqula . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

Iipleyiti ezi-6. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Amacwecwe angama-84 12 anequla . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Amacwecwe angama-84 24 anequla . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 48-iipleyiti zequla. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Amacwecwe angama-84 96 anequla . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Amacwecwe angama-85 384 anequla . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 Iiadaptha zeepleyiti zeWell. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 Iipleyiti zeWell kunye neenkcazo ze-API. . . . . . . . . . . . . . . . . . . . . . . . .87 Ilebhu yepleyiti elungiselelwe kakuhle. . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 5.4 Iingcebiso kunye neerekhi zeengcebiso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 Iirekhi zeengcebiso. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 Ukuhambelana kweTippipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 I-adaptha ye-rack yeengcebiso. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 5.5 Iityhubhu kunye neerekhi zetyhubhu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90 Imibhobho kunye nemidibaniso yeerack . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 6-ityhubhu iiracks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 10-iityhubhu iiracks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 15-ityhubhu iiracks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 24-ityhubhu iiracks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Iinkcazelo ze-API . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Ilebhu yerekhi yesiko . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 5.6 Iibhloko zeAluminiyam. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 Ipleyiti ephantsi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 24 ibhloko ye-aluminiyam yequla. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 96 ibhloko ye-aluminiyam yequla. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Iiadaptha ezizimeleyo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Imidibaniso yelebhu ye-Aluminiyam yebhloko . . . . . . . . . . . . . . . .94 I-24-umdibaniso welebhu ye-aluminiyam ye-aluminiyam kakuhle. . . . . . . . .95 I-96-umdibaniso welebhu ye-aluminiyam ye-aluminiyam kakuhle. . . . . . . . .95 5.7 ILebhu kunye neOpentrons Flex Gripper . . . . . . . . . . . . . . .96 5.8 Iinkcazelo zelebhu eqhelekileyo . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 Ukwenza iinkcazelo zelebhu yesiko . . . . . . . . . . . . . . . . . . .97 Isicwangciso selebhu ye-JSON . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99 JSON iinkcazelo ngelebhu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104 ISahluko 6: Uphuhliso lweProtocol. . . . . . . . . . . . . . . . . . . . . . . . . . .105 6.1 Iiprothokholi ezenziwe kwangaphambili . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105 Ithala leencwadi loMgaqo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Inkonzo ye-105 yoPhuhliso lweSiko loMgaqo. . . . . . . . . . . . . . . .

6.2 uMyili weProtocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109 Iimfuno zoMyili weProtocol . . . . . . . . . . . . . . . . . . . . . . 109 Ukuyila inkqubo yomthetho . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Ukulungiswa kwemigaqo ekhoyo . . . . . . . . . . . . . . . . . . . . . . . . . . 114
6.3 IPython Protocol API . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 Ukubhala nokusebenzisa izikripthi . . . . . . . . . . . . . . . . . . . . . . . . . . . Iimpawu ezikhethekileyo ze-115 Python. . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Iiprothokholi ze-6.4 OT-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Iiprothokholi ze-117 OT-2 Python. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Iiprothokholi ze-118 OT-2 JSON. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Iiprothokholi zeModyuli ezili-120 . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Isahluko 7: Isoftware kunye nokusebenza. . . . . . . . . . . . . . . . . . . . . . . . . . 121 7.1 Ukusebenza kwesikrini esichukumisayo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 Ideshibhodi yerobhothi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 Ulawulo lomgaqo-nkqubo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122 Iinkcukacha zeProtocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124 Yenza useto . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127 Labware Position Check. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128 Ukubaleka inkqubela. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130 Ukugqibezela ukuphumelela. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131 Ulawulo lwesixhobo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132 Useto lweRobhothi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133 Ubume beDekhi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136 7.2 I-Opentrons App. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 Ufakelo lwe-App. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 Ukudluliselwa kwemigaqo kwiFlex. . . . . . . . . . . . . . . . . . . . . . . . . 140 Ubume bemodyuli kunye nolawulo. . . . . . . . . . . . . . . . . . . . . . . . . . . 142 Iprotocol yakutsha nje iqhuba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143 7.3 Ukusebenza okuphezulu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144 iJupyter Notebook. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144 Ukusebenza komgca womyalelo kwi-SSH. . . . . . . . . . . . . . . . . . . . 145
Isahluko 8: ULondolozo kunye neNkonzo. . . . . . . . . . . . . . . . . . . . . . . .147 8.1 Ukucoca iFlex yakho . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Ngaphambi kokuba uqalise . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Into onokuyicoca . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Izisombululo zokucoca. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148 Ukucoca ifreyim kunye nefestile yefestile. . . . . . . . . . . . . . . . . . . . 148 Ukucoca idekhi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148

Ukucoca iGantry. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 Ukucocwa kwenkunkuma. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 8.2 Ukucoca iipayipi neengcebiso . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 Pipette decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Iingcebiso ze-150 zokucoca i-pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151 8.3 Ukucoca into yokubamba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152 8.4 Iimodyuli zokucoca. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 Ukucoca imodyuli ngokubanzi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 Izitywina zeThermocycler. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 8.5 Ilebhu ekhuselekileyo ye-autoclave . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 8.6 I-Service Flex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 Iinkonzo ze-Opentrons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 Isiqinisekiso sofakelo kunye nesiqinisekiso sokusebenza. . . 155 Ukugcinwa kothintelo . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156 Iwaranti . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157 Isihlomelo A: Uluhlu lweenkcazelo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .158 ISihlomelo B: Amaxwebhu olongezelelweyo . . . . . . . . . . . . . . . . . . . . . .170 B.1 I-Opentrons Knowledge Hub . . . . . . . . . . . . . . . . . . . . . . . . . . . . 170 B.2 Amaxwebhu eProtocol yePython API. . . . . . . . . . . . . . . . . . . 170 B.3 Opentrons HTTP API reference. . . . . . . . . . . . . . . . . . . . . . . . 171 B.4 Amaxwebhu omphuhlisi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171 Isihlomelo C: I-Software yoMthombo oVulekileyo . . . . . . . . . . . . . . . . . . . . . . . . .172 C.1 IiOpentrons kwiGitHub. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172 C.2 Opentrons monorepo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173 C.3 Ezinye iindawo zokugcina . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175 IsiHlomelo D: Inkxaso kunye nolwazi loQhagamshelwano . . . . . . . . . . . . . . .176 D.1 Iintengiso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 D.2 Inkxaso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 D.3 Ulwazi loshishino . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177

Intshayelelo
Wamkelekile kwincwadana yemiyalelo ye-Opentrons Flex yokuphatha ulwelo irobhothi. Le ncwadana ikhokelela kuyo yonke into ekufuneka uyazi ukuseta nokusebenzisa iFlex, igxile kwizihloko ezibaluleke kakhulu kubasebenzisi bemihla ngemihla beFlex kwindawo yelebhu.
Isakhiwo sale ncwadana
I-Opentrons Flex yinkqubo entsonkothileyo, ngoko ke zininzi iindlela ezahlukeneyo zokufunda yonke into enokuyenza. Zive ukhululekile ukutsibela ngqo kwisahluko esithetha nangasiphi na isihloko onomdla ngaso! UmzekeloampLe, ukuba sele uneFlex emiselweyo kwilebhu yakho, ungatsiba udlule kwisahluko soFakelo noKufuduswa.
Ukuba ukhetha indlela ekhokelwayo, le ncwadana yakhiwe ukuze ukwazi ukuyilandela ukusuka ekuqaleni ukuya ekupheleni.
Funda ngeFlex. Iimpawu ezahlukileyo zeFleksi zidweliswe kwiSahluko 1: Intshayelelo. Intshayelelo ikwabandakanya ukhuseleko olubalulekileyo nolwazi olulawulayo.
Qalisa ngeFlex. Ukuba ufuna ukuseta iFlex yakho, landela imiyalelo eneenkcukacha kwiSahluko sesi-2: ukuFakelo nokuTyuswa. Emva koko uziqhelanise namacandelo eFlex kwiSahluko sesi-3: Inkcazo yeNkqubo.
Misa umgangatho wakho. Ukuqwalasela umgangatho kuvumela usetyenziso lwezenzululwazi olwahlukeneyo kwiFlex. Isahluko 4: Iimodyuli zichaza iiperipherals ze-Opentrons onokuthi uzifake ngaphakathi okanye ngaphezulu kwedesika ukwenza imisebenzi ethile yesayensi. Isahluko 5: I-Labware ichaza indlela yokusebenza ngezixhobo zokubamba ulwelo.
Yenza iprotocol. Olona setyenziso luphambili lweFlex lusebenzisa iinkqubo zenzululwazi ezisemgangathweni, ezaziwa ngokuba ziiprothokholi. Isahluko sesi-6: Uphuhliso lweProtocol lubonelela ngeendlela ezininzi zokufumana iiprothokholi esele zenziwe okanye uziyile ngokwakho. Ukuqhuba iprotocol yakho, landela imiyalelo kwiSahluko sesi-7: iSoftware kunye nokuSebenza, enemiyalelo yokwenza eminye imisebenzi kunye nokulungelelanisa useto lwerobhothi yakho.
Gcina iFlex isebenza. Landela icebiso elikwiSahluko sesi-8: ULondolozo kunye neNkonzo ukugcina iFlex yakho icocekile kwaye isebenza ngokugqibeleleyo. Okanye ubhalisele enye yeenkonzo ze-Opentrons ezidweliswe apho kwaye usivumele ukuba sikukhathalele iFlex.
Funda ngakumbi. Usafuna enye into? Jonga kwizihlomelo. Isihlomelo A: Uluhlu lweenkcazelo luchaza amagama anxulumene neFlex. Isihlomelo B: Uxwebhu oloNgezelelweyo lukukhomba kwimithombo engaphezulu yeemveliso ze-Opentrons kunye nekhowudi yokubhala yokulawula iFlex.

I-OPENTRONS FLEX

INTSHAYELELO
ISihlomelo C: I-Software ye-Open-Source ichaza indlela i-Opentrons i-software ebanjwe ngayo kwi-GitHub njengesixhobo kubo bobabini abaphuhlisi kunye nabangewona abaphuhlisi.
Isihlomelo D: INkxaso kunye neNgcaciso yoQhagamshelwano idwelisa indlela yokunxibelelana ne-Opentrons ukuba ufuna uncedo olungaphaya koko kunikelwa ngamaxwebhu ethu.
Amanqaku kunye nezilumkiso
Kuyo yonke le ncwadana, uya kufumana amanqaku afomathwe ngokukodwa kunye neebhloko zesilumkiso. Amanqaku anika ulwazi oluluncedo olusenokungabonakali kwindlela eqhelekileyo yokusebenzisa iFlex. Nika ingqalelo ekhethekileyo kwizilumkiso-zisetyenziswa kuphela kwiimeko apho ubaleka umngcipheko wokwenzakala, umonakalo kwisixhobo, ilahleko okanye ukonakaliswa s.ampi-les okanye i-reagents, ilahleko yedatha, okanye enye ingozi. Amanqaku kunye nezilumkiso zijongeka ngolu hlobo:
Sample Qaphela: Le yinto omele uyazi, kodwa ayibeki bungozi.
Sample Isilumkiso: Le yinto ekufuneka uyazi kuba kukho ingozi enxulumene nayo.

I-OPENTRONS FLEX

ISAHLUKO 1
Intshayelelo

Esi sahluko sikwazisa ngeOpentrons Flex ecosystem, kubandakanywa uyilo lwenkqubo iyonke kunye nolungelelwaniso lwendawo yokusebenza. Kukwabandakanya ukuthotyelwa okubalulekileyo kunye nolwazi lokhuseleko, ekufuneka uphindeview phambi kokuseta irobhothi yakho ye-Opentrons Flex. Ukufumana iinkcukacha ezithe vetshe kwiifitsha ze-Opentrons Flex, bona iNgcaciso yeNkqubo isahluko.

1.1 Wamkelekile kwi-Opentrons Flex
I-Opentrons Flex yirobhothi ephethe ulwelo eyilelwe ukuhanjiswa okuphezulu kunye nokuhamba komsebenzi onzima. Irobhothi yeFlex sisiseko senkqubo yemodyuli ebandakanya iipipettes, i-labware gripper, ukulungiswa kwedekhi, iimodyuli ezikwidekhi, kunye ne-labware - yonke into onokuthi uyitshintshe ngokwakho. I-Flex yenzelwe isikrini esichukumisayo ukuze usebenze nayo ngokuthe ngqo kwibhentshi yelebhu, okanye ungayilawula ukusuka kwilebhu yakho yonke nge-Opentrons App okanye ii-API zethu ezivulelekileyo.
Iindawo zokusebenzela zeFlex ziza nazo zonke izixhobo - irobhothi, ihardware, kunye nelebhu - ekufuneka uqalise ukwenza imisebenzi yelebhu eqhelekileyo. Kwezinye usetyenziso, i-Opentrons Flex isebenza kwisoftware evuleleke ngokupheleleyo kunye ne-firmware, kwaye i-reagent- kunye ne-labware-agnostic, ikunika ulawulo kwindlela oyila ngayo kwaye uqhube iiprothokholi zakho.

Yintoni entsha kwiFlex
I-Opentrons Flex yinxalenye yoluhlu lweerobhothi ze-Opentrons zombane. Abasebenzisi be-Opentrons Flex banokuqhelana ne-Opentrons OT-2, irobhothi yethu yobuqu yombhobho. I-Flex ihamba ngaphaya kwamandla e-OT-2 kwiindawo ezininzi eziphambili, ihambisa i-throughput ephezulu kunye nexesha lokuhamba.

Faka iPipette throughput
I-Pipette kunye nezakhono zeengcebiso

Inkcazo
I-Flex pipettes ine-1, 8, okanye i-96 channels. I-pipette ye-96-channel isebenza kumaxesha angama-12 amaninzi amaninzi kanye kanye njenge-OT-2 enkulu yepayipi.
I-Flex pipettes inoluhlu lwevolyum emikhulu (1 µL, 50 µL) kwaye zonke zinokusebenza ngawo nawuphi na umthamo weengcebiso ze-Opentrons Flex. Olu luphuculo ngaphezu kweepipettes ze-OT-5, ezinoluhlu oluncinci kwaye kufuneka lusebenzise iingcebiso ezinoluhlu oluhambelana nomthamo.

I-OPENTRONS FLEX

ISAHLUKO 1: INTSHAYELELO

Gripper Ulungelelwaniso oluzenzekelayo lwesikrini seModyuli yeemodyuli zeDeck slot ilungelelanisa inkunkuma eshukumayo Ubungakanani kunye nobunzima

I-Opentrons Flex Gripper ithatha kwaye ihambise i-labware ngeenxa zonke kwidekhi ngokuzenzekelayo, ngaphandle kokungenelela komsebenzisi. I-gripper ivumela ukuhamba komsebenzi okunzima ngakumbi kwiprotocol enye.
Ukulungelelaniswa kwesikhundla se-Flex pipettes kunye ne-gripper i-automated ngokupheleleyo. Cinezela iqhosha elinye, kwaye isixhobo siya kushukuma siye kwiindawo ezichanekileyo zoomatshini kwidekhi ukumisela eyona ndawo ikuyo, ukugcina loo datha ukuze isetyenziswe kwiiprothokholi zakho.
I-Flex ine-interface yayo yesikrini esichukumisayo esikuvumela ukuba usilawule ngokuthe ngqo, ukongeza ekusebenziseni i-Opentrons App. Sebenzisa isikrini sokuchukumisa ukuqalisa ukusebenza kweprotocol, jonga ubume bomsebenzi, kwaye utshintshe useto ngqo kwirobhothi.
Iimodyuli zeFlex zingena kwiikhadi ezithatha indawo engaphantsi kwedekhi. IiCaddies zibeka ilebhu yakho kufutshane nomphezulu wedekhi kwaye ivumele indlela yentambo engaphantsi kwedekhi. IiCaddies zenza ukuba imodyuli engakumbi kunye noqwalaselo lwelebhu kwindawo yomgangatho.
Iindawo zokubeka iDeck kwi-Flex zibalwe ngenkqubo yolungelelwaniso (A1D4) efana nendlela amaqula afakwe ngayo kwi-labware.
Umgqomo wenkunkuma unokuya kwiindawo ezininzi zedekhi kwiFlex. Indawo emiselweyo (slot A3) yindawo ecetyiswayo. Ungasebenzisa kwakhona into yokubamba ukulahla inkunkuma kwi-chute yenkunkuma ekhethiweyo.
I-Flex inkulu kwaye inzima kakhulu kune-OT-2. Imisebenzi yokufakela kwiFlex ifuna uncedo lweqabane laselebhu.

Uthelekiso oluneenkcukacha lweenkcukacha zobugcisa berobhothi luyafumaneka kwi-Opentrons webindawo.
Zombini iirobhothi zeFlex kunye ne-OT-2 zisebenza kwisoftware yethu evulekileyo, kwaye i-Opentrons App inokulawula zombini iindidi zeerobhothi kanye. Ngelixa iiprothokholi ze-OT-2 zingenakwenziwa ngokuthe ngqo kwiFlex, kulula ukuziqhelanisa (jonga icandelo le-OT-2 Protocols kwisahluko soPhuhliso lweProtocol ukuze ufumane iinkcukacha).

Flex workstations
Iindawo zokusebenzela ze-Opentrons Flex ziquka i-Flex irobhothi, izincedisi, iipayipi kunye ne-gripper, iimodyuli ezikwi-deck, kunye ne-labware efunekayo ukuzenzekelayo isicelo esithile. Onke amacandelo okusebenzela akwimodyuli. Ukuba ufuna ukutshintsha usetyenziso, unokongeza okanye utshintshe kwezinye izixhobo zeFlex kunye nezinto ezisetyenziswayo ezihambelanayo.

I-OPENTRONS FLEX

ISAHLUKO 1: INTSHAYELELO
INDAWO YOMSEBENZI YE-NGS
I-Opentrons Flex i-NGS Workstation yenza i-NGS isebenze ngokuzenzekela ukulungiselela ithala leencwadi. Iyakwazi ukwenza ngokuzenzekelayo ukuhamba komsebenzi kwangaphambili usebenzisa nayiphi na inkqubo ye-reagent ehamba phambili, kuquka ukuhlukana- kunye tagUkulungiselela ithala leencwadi elisekelwe kwingcaciso.
Ukongeza kwirobhothi yeFlex, i-NGS Workstation ibandakanya:
I-Gripper Ukukhethwa koqwalaselo lwepipette
IiPipette ezimbini ze-8-Channel (1 µL kunye ne-50 µL) i-5-Channel Pipette (1000 µL) iNkunkuma yeChute Magnetic Block Temperature yeModyuli yeThermocycler Ikhithi yeLabware yeModyuli enengcebiso, iityhubhu zemicrocentrifuge, iireservoirs, kunye neepleyiti zePCR.
PCR UMSEBENZI
I-Opentrons Flex PCR Workstation yenza ngokuzenzekelayo ukuseta iPCR kunye nokuhamba komsebenzi we-thermocycling ukuya kuthi ga kwi-96 s.ampLes. Iyakwazi aliquot reagents chilled kunye sampLes kwipleyiti yePCR engama-96. Ngokudityaniswa kweModyuli yeThermocycler ezenzekelayo, sebenzisa igripper ukulayisha ipleyiti kwiThermocycler, kwaye emva koko uqhube inkqubo oyikhethileyo yePCR.
Ukongeza kwirobhothi yeFlex, iPCR Workstation ibandakanya:
I-Gripper Ukukhethwa koqwalaselo lwepipette
I-1-Channel Pipette (1 µL) kunye ne-50-Channel Pipette (8 µL) 1-Channel Pipette (50 µL) Inkunkuma yeChute yoBubushushu beModyuli yekiti yeLabware enengcebiso, iityhubhu zemicrocentrifuge, iireservoirs, kunye neepleyiti zePCR.

I-OPENTRONS FLEX

ISAHLUKO 1: INTSHAYELELO
I-NUCLEIC Acid ExtraCTION UMSEBENZI WOKUSEBENZA
I-Opentrons Flex Nucleic Acid Extraction Workstation yenza i-DNA / i-RNA yodwa kunye nokuhlanjululwa. Isebenzisa iBhloko yeMagnetic yokwahlula amaso emagnethi, kunye neHeater-Shaker yesample lysis kunye nokumiswa kwamaso kazibuthe.
Ukongeza kwirobhothi yeFlex, iNucleic Acid Extraction Workstation ibandakanya:
I-Gripper Ukukhethwa koqwalaselo lwepipette
I-1-Channel Pipette (5 µL) kunye ne-1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL) Inkunkuma Chute Magnetic Block Heater-Shaker Imodyuli yeLabware ikiti enengcebiso zokucoca, iireservoir, iipleyiti zePCR ezinzulu, kunye neepleyiti zequla ezinzulu zePCR.
I-MAGNETIC BEAD YOMSEBENZI WOKUSEBENZA
I-Opentrons Flex iMagnetic Bead yeProtein yokuCoca iZiko lokuSebenza lizenza ngokuzenzekelayo ukucocwa kweprotheyini encinci kunye neproteomicsampndilungiselele ukuya kuthi ga kwi-96samples. Iyahambelana nezinto ezininzi ezidumileyo ezisekelwe kwi-magnetic-bead reagents.
Ukongeza kwirobhothi yeFlex, iProtein Purification Workstation ibandakanya:
I-Gripper Ukukhethwa koqwalaselo lwepipette
I-1-Channel Pipette (5 µL) kunye ne-1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL) Inkunkuma Chute Magnetic Block Heater-Shaker Imodyuli yeLabware ikiti enengcebiso zokucoca, iireservoir, iipleyiti zePCR ezinzulu, kunye neepleyiti zequla ezinzulu zePCR.
FLEX PREP UMSEBENZI WOKUSEBENZA
I-Opentrons Flex Prep Workstation yenza ngokuzenzekelayo ukuhamba kombhobho okulula. Lungisa indawo yokusebenzela nge-1-Channel kunye ne-8-Channel Pipettes ukwenza imisebenzi efana ne-sample transfer, sample ukuphindaphinda, kunye

I-OPENTRONS FLEX

ISAHLUKO 1: INTSHAYELELO
reagent aliquoting. Qwalasela indawo yokusebenzela nge-96-Channel Pipette ukwenza i-reagent ephumayo ephezulu kunye ne-plate stampnge.
Ukongeza kwirobhothi yeFlex, iFlex Prep Workstation ibandakanya:
Ukukhethwa kobumbeko lwepipette: 1-Channel Pipette (5 µL) kunye ne-1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL)
Ikhithi yeLebhu eneengcebiso zokucoca, iityhubhu ze-microcentrifuge, kunye neendawo zokugcina amanzi
I-PLASMID PREP YOKUSEBENZA
I-Opentrons Flex Plasmid Prep Workstation yenza i-automate-based-bead-based plasmid extraction kunye nokuhamba komsebenzi wokucoca. Esi sixhobo sokusebenzela sixhotyiswe ngeepipette ezinomthamo ophezulu, iModyuli ye-Heater-Shaker, kunye neBhloko yeMagnetic ukulungiselela uninzi lwekhemistri esekwe ngamaso.
Ukongeza kwirobhothi yeFlex, iPlasmid Prep Workstation ibandakanya:
I-Gripper 1-Channel Pipette (5 µL) kunye ne-1000-Channel Pipette (8 µL) iNkunkuma yeChute Magnetic Block Heater-Shaker Imodyuli yebhuthi yeLabware yeengcebiso, iityhubhu zemicrocentrifuge, iireservoirs, iipleyiti zePCR, kunye neepleyiti zequla elinzulu.
I-SYNBIO YOMSEBENZI
I-Opentrons Flex I-SynBio Workstation yenza ngokuzenzekelayo iindidi zebhayoloji yokwenziwa komsebenzi efana ne-DNA synthesis kunye ne-cloning. Isebenzisa iBhloko yeMagnetic kunye neModyuli yobushushu ukuxhasa uninzi lwekhemistri esekwe ngamaso. Yongeza iModyuli ye-Thermocycler ukwenza i-incubations ye-lid eshushu kunye ampliifications.
Ukongeza kwirobhothi yeFlex, iSynBio Workstation ibandakanya:
I-Gripper ye-1-Channel Pipette (5 µL) kunye ne-1000-Channel Pipette (8 µL) iMagnetic Block Temperature Module

I-OPENTRONS FLEX

ISAHLUKO 1: INTSHAYELELO

Ikhithi yeLabware enamacebiso aqhelekileyo kunye nesihluzi, iityhubhu zemicrocentrifuge, iireservoirs, iipleyiti zePCR, kunye neepleyiti zequla ezinzulu.

1.2 Ulwazi ngokhuseleko
I-Opentrons Flex yokuphatha ulwelo irobhothi yenzelwe ukusebenza ngokukhuselekileyo. Jonga kwiinkcukacha kunye nezikhokelo zokuthotyelwa kweli candelo ukuqinisekisa ukusetyenziswa ngokukhuselekileyo kweFlex yakho. Ezi zikhokelo zibandakanya ukusetyenziswa ngokukhuselekileyo kwegalelo kunye noqhagamshelo lwemveliso, kubandakanywa amandla kunye noqhagamshelwano lwedatha, kunye neelebhile zesilumkiso ezifunyenwe kwi-Flex robot kunye ne-hardware ehambelana nayo. Ukusebenzisa isixhobo ngendlela engeyiyo leyo ichazwe kule ncwadana inokubeka umsebenzisi kunye nesixhobo emngciphekweni.

Iimpawu zokhuseleko
Iilebhile ezahlukeneyo kwiFlex nakule ncwadana ziyakulumkisa malunga nemithombo yokwenzakala okanye ukwenzakala okunokwenzeka.

Uphawu

Inkcazo
Isilumkiso: Ulumkisa abasebenzisi ngeemeko ezinokuthi zibe yingozi. Izenzo ezinokukhokelela kukwenzakala okanye ukufa komntu.
Isilumkiso: Ilumkisa abasebenzisi ngomonakalo kwisixhobo. Idatha elahlekileyo okanye eyonakeleyo. Uphazamiseko olungafumanekiyo lotyando olwenziwayo.
Ukothuka kombane: Ichonga izixhobo zesixhobo ezinokubeka umngcipheko wokothuso lombane ukuba isixhobo siphathwa ngendlela engafanelekanga.

Umphezulu oshushu: Ichonga izixhobo zesixhobo ezibeka umngcipheko wokwenzakala ngenxa yobushushu obuphezulu/amaqondo obushushu ukuba isixhobo siphathwa ngendlela engafanelekanga.

Indawo yePinch: Ichonga izixhobo zesixhobo ezinokubeka umngcipheko wokwenzakala xa ushukuma.

I-OPENTRONS FLEX

ISAHLUKO 1: INTSHAYELELO

Uya kufumana ezi lebhile zilandelayo kwiFlex:
Ieleyibhile zepropathi enomgangatho ophezulu wokuqonda Ielebhile zothotyelo lolawulo (umzekelo, ETL) Ielebhile zengozi yombane Ielebhile ezilumkisa ngokubanzi Ieleyibhile zemveliso IPinch point iilebhiletage iilebhile iilebhile zokukala Amandla

Izilumkiso zokhuseleko lombane
Soloko uqwalasela ezi zilumkiso zokhuseleko zombane zilandelayo:

Uphawu

Inkcazo
Qhagamshelana irobhothi kwindawo esekelwe phantsi, yeKlasi yoku-1 yesekethe. Jonga icandelo loQhagamshelwano lwaMandla kwiNgcaciso yeNkqubo isahluko.

Musa ukuqhagamshela (iplagi ngaphakathi), qhawula (khulula), okanye usebenzise iintambo zombane ze-AC ukuba: Intambo yonakele okanye yonakele. Ezinye iintambo ezincanyathiselweyo, iintambo, okanye izinto zokungenisa izinto zonakele okanye zonakele.
Ukusebenzisa iintambo zamandla ezonakalisiweyo kunokubangela ingozi yokutshatyalaliswa kombane ekhokelela kukwenzakala okukhulu okanye umonakalo kwirobhothi.
Musa ukutshintsha intambo yombane ye-AC ngaphandle kokuba usiya kwicala leNkxaso ye-Opentrons.

Ngolwazi oluthe vetshe ngeemfuno zombane, jonga icandelo lokuSetyenziswa koMbane kwisahluko soFakelo nokuHambisa.

I-OPENTRONS FLEX

ISAHLUKO 1: INTSHAYELELO

Izilumkiso ezongezelelweyo zokhuseleko
Soloko uqwalasela ezi zilumkiso zongeziweyo zilandelayo:

Uphawu

Inkcazo
I-Opentrons Flex ayiqinisekiswanga ukuba ingasetyenziswa nolwelo oluqhumayo okanye olunokutsha. Musa ukulayisha iipleyiti, iityhubhu, okanye iingqayi ezinolwelo oluqhumayo okanye olunokutsha kwirobhothi okanye usebenzise isixhobo ngolwelo oluqhumayo okanye olunokutsha kwindawo ebiyelweyo.
Sebenzisa iindlela ezifanelekileyo zelabhoratri kwaye ulandele izilumkiso zomenzi xa usebenza ngemichiza. I-Opentrons ayinaxanduva okanye inoxanduva lwawo nawuphi na umonakalo ngenxa, okanye ngenxa yokusetyenziswa kweekhemikhali eziyingozi.

I-Flex inobunzima be-88.5 kg (195 lbs). Ngenxa yoko, kufuna abantu ababini ukuba bayiphakamise kwaye bayihambise ngokukhuselekileyo. Jonga icandelo lokuFudusa kwisahluko soFakelo nokuFudusa.

I-Flex kufuneka ibekwe kumphezulu okwaziyo ukuxhasa ubunzima bayo be-88.5 kg (195 lbs) kunye nendawo eyaneleyo yomphezulu ukuhlalisa irobhothi kunye nomgama wayo osezantsi wokucoceka (20 cm/8 in). Jonga icandelo loKhuseleko kunye neeMfuno zokuSebenza kwisahluko sokuFakelo nokuFudusa.
I-Flex inokukhupha iintshukumo ngelixa isebenza. Beka irobhothi kumphezulu owomeleleyo, okwinqanaba, nongevani namanzi kunye nezibophelelo ezinqamlezileyo okanye ezidityanisiweyo. Jonga icandelo loKhuseleko kunye neeMfuno zokuSebenza kwisahluko sokuFakelo nokuFudusa.

Izilumkiso zokhuseleko
Ukunceda ukukhusela iFlex kumonakalo, landela la manyathelo okhuseleko:

I-OPENTRONS FLEX

ISAHLUKO 1: INTSHAYELELO

Uphawu

Inkcazo
Sebenzisa ilebhu ehambelana ne-ANSI/SLAS okanye evunywe yi-Opentrons. Jonga isahluko seLabware.
Gcina imathiriyeli eyonakalisayo, iiarhente, okanye ezinye izinto ezonakalisayo kude nerobhothi.

Ukhuseleko lwebhayoloji
Nyanga iisampulu kunye nee-reagents ezinemathiriyeli ethathwe ebantwini njengee-arhente ezinokosuleleka. I-Opentrons icebisa ukuba kusetyenziswe iinkqubo zelabhoratri ezikhuselekileyo njengoko kuchaziwe kwi-Biosafety kwi-Microbiological and Biomedical Laboratories (BMBL) 6th Edition.
Kwiimeko eziqhelekileyo, i-Flex ayidali i-aerosols ebonakalayo evela kumthombo wolwelo. Nangona kunjalo, phantsi kweemeko ezithile, kuyenzeka ukuvelisa ii-aerosols kulwelo lwemithombo. Xa usebenza nge-biosafety level 2 okanye ulwelo olungaphezulu komthombo, cinga ukuthatha amanyathelo okhuseleko malunga nokuba sesichengeni se-aerosol, ngokuhambelana nezigqeba ezilawulayo zengingqi yakho. Ukunciphisa umngcipheko wokuvezwa kwe-aerosol kwirobhothi, qinisekisa ukuba:
Yenza ulondolozo njengoko kuchaziwe kwisahluko seSondlo neNkonzo. Faka ngokufanelekileyo kwaye ukhusele zonke izixhobo zokugubungela, iipayipi, iimodyuli, kunye ne-labware. Sebenzisa ubuchule obufanelekileyo bokufaka imibhobho ukunceda ukuthomalalisa ii-aerosols.
Umsi onetyhefu
Ukuba usebenzisa izinyibilikisi eziguquguqukayo okanye izinto eziyityhefu, sebenzisa isixokelelwano sokuphefumla selabhoratri esisebenzayo ukususa nawuphi na umphunga onokuthi uveliswe.
Ulwelo olunokutsha
I-Flex ayizange ivavanywe ukuba isetyenziswe ngolwelo olunokutsha kwaye akufanele isetyenziswe kunye nolwelo olunokutsha.

I-OPENTRONS FLEX

ISAHLUKO 1: INTSHAYELELO

1.3 Ukuthotyelwa kwemigaqo
I-Opentrons Flex ithobela zonke iimfuno ezisebenzayo zale migangatho ilandelayo yokhuseleko kunye nemigangatho yombane wombane.

Ukhuseleko
Rule ID IEC/UL/CSA 61010-1 IEC/UL/CSA 61010-2-051

Isihloko
IiMfuneko zoKhuseleko kwiSixhobo soMbane soMlinganiso, uLawulo, kunye nokuSetyenziswa kweLabhoratri iCandelo 1: IiMfuno ngokuPhangaleleyo
Iimfuno ezikhethekileyo zezixhobo zebhubhoratri zokuxuba kunye nokuvuselela

Ukuhambelana kwe-electromagnetic

Rule ID EN/BSI 61326-1
I-FCC 47 CFR Icandelo le-15 Icandelwana B Udidi A lwe-IC ICES-003

Isihloko
IsiXhobo soMbane soMlinganiso, uLawulo, kunye nokuSetyenziswa kweLabhoratri yeeMfuno ze-EMC iSahlulo soku-1: IiMfuno ngokuPhangaleleyo ngokuNgenanjongo
ULawulo lweSpectrum kunye noNxibelelwano loNxibelelwanoIsiXhobo soMgangatho weSixhobo seTekhnoloji yeSixhobo seTekhnoloji (kubandakanywa neDijithali yeDijithali)

Izilumkiso zeFCC kunye namanqaku
Isilumkiso: Utshintsho okanye uhlengahlengiso kule yunithi engavunywanga ngokucacileyo yi-Opentrons inokutshitshisa igunya lomsebenzisi lokusebenzisa isixhobo.
Esi sixhobo sithobela iCandelo le-15 lemithetho yeFCC. Umsebenzi uxhomekeke koku kulandelayo:

I-OPENTRONS FLEX

ISAHLUKO 1: INTSHAYELELO
Esi sixhobo sisenokungabangeli uphazamiseko oluyingozi. Esi sixhobo kufuneka samkele naluphi na uphazamiseko olufunyenweyo, kubandakanywa uphazamiseko olunokubangela okungafunekiyo
ukusebenza.
Qaphela: Esi sixhobo sivavanyiwe kwaye safunyanwa sithobela imida yesixhobo sedijithali soDidi A, ngokungqinelana neCandelo le-15 lemithetho yeFCC. Le mida yenzelwe ukubonelela ngokhuseleko olufanelekileyo ekuphazamisekeni okuyingozi xa isixhobo sisebenza kwindawo yorhwebo. Esi sixhobo sivelisa, sisebenzise, kwaye sinokusasaza amandla erediyo yerediyo kwaye, ukuba asifakanga kwaye sisetyenziswa ngokuhambelana nencwadana yemiyalelo, sinokubangela uphazamiseko oluyingozi kunxibelelwano lwerediyo. Ukusebenza kwesi sixhobo kwindawo yokuhlala kunokubangela uphazamiseko oluyingozi kwimeko apho umsebenzisi kuya kufuneka alungise uphazamiseko ngeendleko zakhe.
Canada ISED ukuthotyelwa
EKhanada ICES-003(A) / NMB-003(A)
Le mveliso idibana ne-Innovation esebenzayo, iSayensi kunye noPhuhliso loQoqosho lwaseKhanada iinkcukacha zobugcisa.
Le présent produit est conforme aux spécifications techniques ezisebenzayo kwi-Innovation, Sciences et Developpement économique Canada.
Isilumkiso ngokusingqongileyo
Isilumkiso: Umhlaza kunye Nokwenzakala kwiNzala www.P65Warnings.ca.gov
Wi-Fi precertification
Imodyuli ye-Wi-Fi iqinisekisiwe ukuba isetyenziswe kwimimandla emininzi:
EUnited States (FCC): Isazisi seFCC UAY-W8997-M1216 Ummandla woQoqosho waseYurophu (CE): Akukho sichongi sikawonke-wonke (ukuzichaza) eKhanada (IC): Inombolo yeNguqulelo yeHardware yeSazisi W8997-M1216 Japan (TELEC): Inombolo eqinisekisiweyo 020-170034 I-Indiya (WPC): Inombolo yobhaliso ETA-SD-20191005525 (ukuzichaza)

I-OPENTRONS FLEX

ISAHLUKO 2
Ukufakela kunye nokuFudusa
Esi sahluko sichaza indlela yokulungiselela ilebhu yakho ye-Opentrons Flex, indlela yokuseta irobhothi, kunye nendlela yokuyihambisa ukuba kuyimfuneko. Phambi kokuba uthathe iFlex yakho, qiniseka ukuba ilebhu okanye indawo osebenza kuyo iyahlangabezana nazo zonke iikhrayitheriya ezikwicandelo loKhuseleko kunye neMiqathango yokuSebenza. Xa ilixesha lokufumana iFlex yakho isebenze, landela imiyalelo eneenkcukacha kwi-Unboxing, i-First Run, kunye ne-Instrument Installation kunye ne-Calibration, okanye usebenzise inkonzo ye-Opentrons Onsite Support Set Up. Kwaye ukuba ufuna ukuhambisa iFlex yakho kwindawo entsha, kufutshane okanye kude, landela amanyathelo akwicandelo lokuHanjiswa.
2.1 Iimfuno zokhuseleko kunye nokusebenza
Indawo yokubeka i-Opentrons Flex
Indawo yimpahla exabisekileyo phantse kuyo yonke ilebhu. I-Flex yakho iza kufuna enye-kodwa hayi kakhulu, njengoko yenzelwe ukuba ilingane kwisiqingatha sebhentshi yelebhu eqhelekileyo. Qinisekisa ukuba unendawo ehambelana nezi mfuno zilandelayo.
Umphezulu webhentshi: Umphezulu omileyo, owomeleleyo, othe tyaba, umphezulu ongenamanzi. Iitafile okanye iibhentshi ezinamavili (nokuba ngamavili okutshixa) azikhuthazwa. I-Flex ihamba ngokukhawuleza kwaye inobunzima obuninzi, obunokugubha okanye ukungalingani kwetafile ekhaphukhaphu okanye eshukumayo.
Ukuthwala ubunzima: Irobhothi iyodwa inobunzima be-88.5 kg (195 lb) kwaye kufuneka iphakanyiswe kuphela ngabantu ababini abasebenza kunye. Beka irobhothi kwindawo enokuxhasa ubunzima bayo ngokulula kunye nobunzima bazo naziphi na iimodyuli, ilebhu, ulwelo, okanye ezinye izixhobo zaselebhu eziza kusetyenziswa kwizicelo zakho.
Indawo yokusebenza: Imilinganiselo yesiseko serobhothi yi-87 cm W x 69 cm D x 84 cm H (malunga ne-34 "x 27" x 33 "). I-Flex idinga i-20 cm (8″) yokukhutshwa kwecala nasemva kwiintambo, uqhagamshelo lwe-USB, kunye nokukhupha umsi kwiimodyuli ezishushu kwaye zipholile.
Isilumkiso: Musa ukubeka macala okanye ngasemva kweFlex egungxulwayo eludongeni.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA

84cm 33″

87cm 34″
Opentrons Flex isiseko imilinganiselo.

69cm 27″

20cm 8″

Phezulu view ye-Opentrons Flex, ebonisa ubuncinci becala kunye nokukhutshwa ngasemva.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA

Ukutya umbane
I-Opentrons Flex kufuneka iqhagamshelwe kwindawo yokuphuma eludongeni okanye kufutshane nendawo yebhentshi oyifaka kuyo. Qhagamshela iFlex kuphela kwiisekethe ezinokuthatha amandla ayo aphezulu:
Amandla okufakwayo: 36 VDC, 6.1 A Ukusetyenziswa ngokungasebenziyo: 30 W Ukusetyenziswa okuqhelekileyo: 40 W (ngexesha lokuqhuba kweprotocol) Ukusetyenziswa okuphezulu: Ngokumalunga ne-50 W
Ukusetyenziswa kwamandla ngqo kuxhomekeke kwi:
Ubungakanani kunye nohlobo lwentshukumo eyenziwa ngexesha lomgaqo olandelwayo. Ixesha elichithwa yirobhothi ingenzi nto. Ubume bezibane kwirobhothi. Zingaphi izixhobo ezincanyathiselweyo.
Khumbula ukuphendula ngezinye ii-electronics ezisebenzisa amandla kwisekethe enye, kubandakanywa iimodyuli zeFlex ezinezixhobo zabo zamandla. Umzekeloample, iModyuli yeThermocycler inokusetyenziswa kwamandla aphezulu (630 W) okungaphezulu kakhulu kunerobhothi yeFlex ngokwayo. Ukuba kuyimfuneko, dibana nomphathi weziko lakho ukuqinisekisa ukuba liyahlangabezana neemfuno zamandla zesixhobo sakho.

Iimeko zokusingqongileyo
Iimeko zokusingqongileyo zokusetyenziswa okucetyiswayo, ukusetyenziswa okwamkelekileyo, kunye nokugcinwa kuyahluka:

Icetyiselwe ukusebenza kwenkqubo

Yamkelekile ekusebenzeni kwenkqubo

Ubushushu be-Ambient +20 ukuya +25 °C

+2 ukuya +40 °C

Ukufuma okuhambelanayo Umphakamo

40%, non-condensing
Malunga nama-500 m ngaphezu komphakamo wolwandle

I-30%, i-non-condensing (ngaphantsi kwe-80 °C)
Ukuya kuthi ga kwi-2000 m ngaphezu komphakamo wolwandle

Ukugcinwa kunye nokuthutha
-10 ukuya +60 °C
I-10%, i-non-condensing (ngaphantsi kwe-85 °C)
Ukuya kuthi ga kwi-2000 m ngaphezu komphakamo wolwandle

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
I-Opentrons iqinisekise ukusebenza kwe-Opentrons Flex kwiimeko ezicetyiswayo ekusebenzeni kwenkqubo, kwaye ukusebenza kuloo meko kufuneka kunikeze iziphumo ezilungileyo. I-Flex ikhuselekile ukusetyenziswa kwiimeko ezamkelekileyo ekusebenzeni kwenkqubo, kodwa iziphumo zinokwahluka. Musa ukusebenzisa okanye ukusebenzisa iFlex kwiimeko ezingaphandle kwaloo mida. Iimeko zokugcina kunye nokuthutha zisebenza kuphela xa irobhothi ikhutshwe ngokupheleleyo kumandla kunye nezinye izixhobo.
2.2 Ukuvula ibhokisi
Sivuyisana nawe! I-Opentrons Flex yakho ifikile kwaye uyilungiselele isithuba kwilebhu yakho. Masivule laa crate ye-monster, sisuse irobhothi, kwaye siyilungiselele ukusebenza. Ulwazi olukweli candelo lubonelela ngoluhlu lwamalungu kunye nemiyalelo ehamba nawe kumanyathelo afunekayo ukuze ufumane iFlex ingenabhokisi, imiselwe, kwaye ilungele ukusetyenziswa. Siye sahlula inkqubo yokuseta ibe ngamacandelo amathathu:
Icandelo loku-1 libandakanya ukuchithwa kwekhreyithi. Icandelo lesi-2 libandakanya ukususa i-Flex kwikhreyithi kwaye iyise kwindawo yokudibanisa yokugqibela. Icandelo lesi-3 libandakanya indibano yokugqibela kunye nokwenza amandla kwirobhothi okokuqala.
Umzamo kunye nexesha elifunekayo
Uyakufuna ukucela iqabane laselebhu ukuba lincedise ngokukhupha ibhokisi, ukuphakamisa, ukuhambisa, kunye nenkqubo yokuhlanganisa. Kuya kufuneka wenze uhlahlo lwabiwo-mali malunga nemizuzu engama-30 ukuya kwiyure ukwenza lo mzamo.
Qaphela: I-Flex ifuna abantu ababini ukuyiphakamisa kakuhle. Kwakhona, ukuphakamisa nokuthwala iFlex ngezibambo zayo yindlela efanelekileyo yokuhambisa irobhothi.
Ikhreyithi kunye nezinto zokupakisha
Ukukhupha iFlex kukunika irobhothi eyoyikekayo, kodwa ushiyeke neepaneli ezininzi ezinkulu zekhreyithi kunye nezinto ezidityanisiweyo zokuthumela kunye neepadding. Ngoxa unokuwulahla lo mbandela, siyakukhuthaza ukuba uzigcine ezi zinto ukuba indawo yokugcina ikhona. Ipakethe iyasebenziseka kwakhona, nto leyo enceda ekulungiseleleni iFlex yakho yokuthunyelwa ngenqanawa ukuba ngaba ufuna ukuyithumela kwenye indawo (umzekelo, kwinkomfa okanye kwindawo entsha) kwixesha elizayo.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
Izinto zemveliso
Iinqanawa zeFlex ezinamacandelo adweliswe ngezantsi. IiPipettes, igripper, kunye neemodyuli zingena kwipakethe eyahlukileyo ukusuka kwikhreyithi yeFlex engundoqo, nokuba uzithenge kunye njengendawo yokusebenzela.

(1) Irobhothi ye-Opentrons Flex

(1) Intambo ye-USB

(1) Intambo yombane

(1) Intambo ye-Ethernet

(5) Izitshixo ze-L (12 mm hex, 1.5 mm hex, 2.5 mm hex, 3 mm hex,
T10 Torx)

(1) I-Emergency Stop Pendant

(1) Indawo yokubeka enekliphu zelebhu

(4) Iikliphu zeelebhu ezisecaleni

(1) Iprobe yokulinganisa iPipette

(4) Ukuthwala imiqheba neminqwazi

(1) Iphaneli yefestile ephezulu

(4) Iipaneli zefestile ezisecaleni

(1) 2.5 mm hex screwdriver

(1) 19 mm isitshisi

(16 + izikhonkwane) Izikrufu zefestile (M4x8 mm intloko ecaba)
26

(10) Izikrufu zedekhi esecaleni (M4x10 mm intloko yesokethi)

(12) Izikrufu zedekhi ezisecaleni (intloko yesokethi ye-M3x6 mm)

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
Isigaba 1: Susa ikhreyithi
I-Opentrons ithumela iFlex yakho kwikhreyithi eyomeleleyo yeplywood. Ikhreyithi yokuthumela ngenqanawa isebenzisa ihuku kunye ne-latch clamps ukukhusela phezulu, icala, kunye neepaneli ezisezantsi kunye. Ukusebenzisa iiletshi, endaweni yezikhonkwane okanye izikrufu, kuthetha ukuba awuyi kufuna i-crowbar (okanye amandla amaninzi) ukuqhawula ikhreyithi, kwaye unokuphinda uyidibanise kamva, ukuba iyafuneka.
Qaphela: Imiphetho yekhreyithi inokuba rhabaxa ngexesha lokuthumela ngenqanawa. Unokufuna ukusebenzisa iiglavu zomsebenzi ukukhusela izandla zakho kwiiplanga zokhuni.
Ukukhulula iiletshi, flip i-latch tab phezulu kwaye uyijike ngasekhohlo (ngokuchasene ne-clockwise). Esi senzo sishukumisa i-clamp ingalo iphuma kwisibiyeli sayo esihambelanayo. Emva koko ungajika ingalo yelatshi uyisuse kwikreyithi.
1 Vula iiletshi ezisibhozo ubambe umphezulu emacaleni.

2 Susa ipaneli ephezulu emva kokukhulula iiletshi.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKEKELWA NOKUTSHINTSHA 3 Sika uvule ibhegi yokuthumela eluhlaza okwesibhakabhaka, susa ezi zinto kwi-padding, kwaye uzibeke ecaleni:
Amandla eKit yomsebenzisi, i-Ethernet, kunye neentambo ze-USB i-Emergency Stop Pendant
4 Susa iqhekeza eliphezulu lephedi yogwebu ukuveza iipaneli zefestile. I-padding ikhusela icala kunye neepaneli eziphezulu.
5 Susa iipaneli zefestile uze uzibeke ecaleni. Uzakuncamathisela ezi kamva.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
6 Vula iiletshi ezili-16 ezishiyekileyo ubambe iipaneli ezisecaleni enye kwenye kunye nesiseko sekhreyithi. 7 Susa iipaneli ezisecaleni uze uzibeke ecaleni.
Icandelo 2: Khupha iFlex
Emva kokugqiba amanyathelo akwiNqanaba loku-1, ngoku kufuneka ubone irobhothi ekwibhegi ekhuselayo kwaye incamathele kwizinto ezifakwa kwintsimbi eorenji. Ibhegi ivale irobhothi kwaye iyikhusele kwindawo engaphandle. Izibiyeli zentsimbi zikhusela irobhothi ukuya ezantsi kwekhreyithi. Iifreyimu ezimbini zokuthumela zixhasa irobhothi, isasaza ubunzima bayo ngokulinganayo, kwaye iyigcine iqinile ukuze ingajiki ngexesha lokuthumela. Qhubeka nokukhupha iFlex kwaye uyisuse kwisiseko sekreyiti.
8 Usebenzisa i-19 mm isitshisi kwiKiti yoMsebenzisi, khulula izibiyeli ukusuka ezantsi kwekhreyithi. Ungalahla izibiyeli, okanye uzigcinele ukusetyenziswa kwixesha elizayo.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUBUYISA 9 Tsala okanye uqengqe ibhegi yokuthumela yonke indlela ezantsi ukuveza irobhothi yonke.
10 Ngoncedo oluvela kwiqabane lakho laselabhu, bamba ukubamba kwiifreyim eziorenji zokuthumela macala omabini esiseko serobhothi, phakamisa iFlex kwisiseko sekreyithi, kwaye uyibeke phantsi emgangathweni. Gcina okanye ulahle isiseko sekhreyithi kunye nesakhelo sokuthumela.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUBUYISELWA KWI-11 Ukusebenzisa i-12 mm hex isitshixo se-L kwiKiti yoMsebenzisi, susa iibholiti ezine ezibambe izakhelo zokuthumela ukuze
iFlex. Gcina okanye ulahle iifreyimu kunye neebholithi.
12 Susa iziphatho ezine ze-aluminiyam kwiKiti yoMsebenzisi. Krwela iziphatho kwiindawo ezifanayo ezibambe iibholithi zesakhelo sokuthumela eziyi-12 mm.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
13 Ngoncedo oluvela kwiqabane lakho laselebhu, phakamisa iFlex ngeziphatho zayo ze uyibeke kwindawo yokusebenza ukuze uyihlanganise okokugqibela.

Icandelo 3: Indibano yokugqibela kunye namandla avuliwe
Emva kokuhambisa iFlex kwindawo yokusebenza yethutyana, okanye ikhaya layo elisisigxina, lixesha lokuba ubeke ukugqiba kwirobhothi yakho entsha.
14 Ukuba uye wahambisa irobhothi kwindawo yayo yokugqibela, yokusebenza, susa izibambo eziphetheyo kwaye ubeke endaweni yazo ngeminqwazi yokugqiba. Iminqwazi ivala izibambo zokuvula kwisakhelo kwaye inike irobhothi imbonakalo ecocekileyo. Buyisela iziphatho kwiKiti yoMsebenzisi ukuze ugcinwe.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
15 Khupha umphezulu kunye neepaneli ezisecaleni kwi-foam yokupakisha oyibeke bucala emva kokususa umphezulu wekhreyithi.
16 Faka iipaneli zefestile kwiFlex ngokulandela ulwazi lwelebhile kwifilimu ekhuselayo yangaphambili. Emva koko susa ifilimu ekhuselayo.
17 Ukusebenzisa izikrufu zefestile ezibeveled kunye ne-screwdriver eyi-2.5 mm ukusuka kwiKit yomsebenzisi, ncamathela iipaneli zefestile kwiFlex. Qinisekisa ukuba imingxuma ebhentshiweyo (emilo engu-V) kwiiphaneli zefestile ijonge ngaphandle (ngawe). Oku kuvumela izikrufu ukuba zilingane nokugungxulwa komphezulu wefestile.

Isilumkiso: Ukungaziqondi kakuhle iiphaneli kunokukhokelela kumonakalo. I-screw torque egqithisileyo inokuqhekeka iipaneli. Isandla qinisa izikrufu de iipaneli zefestile zikhuseleke ngokufanelekileyo. Olu asilovavanyo lwamandla.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUBUYISELWA KWI-18 Ukusebenzisa i-screwdriver ye-2.5 mm kwiKiti yoMsebenzisi, susa izikrufu zokutshixa kwi-gantry. Ezi
izikrufu zinqanda i-gantry ukuba ihambe xa isendleleni. Izikrufu zokutshixa igantry zifumaneka: Kwicala lasekhohlo likaloliwe kufutshane nomphambili werobhothi. Ngaphantsi kwengalo ethe nkqo ye-gantry. Kwicala lasekunene likaloliwe kufutshane nomphambili werobhothi kwisibiyeli esiorenji. Kukho izikrufu ezibini apha.
I-gantry ihamba ngokulula ngesandla emva kokususa zonke izikrufu zokuthumela. 19 Sika kwaye ususe amabhanti amabini erabha abambe umgqomo wenkunkuma ngexesha lokuthumela.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
20 Qhoboshela intambo yombane kwiFlex uze uyiplage kwindawo ephuma eludongeni. Qinisekisa ukuba indawo yedekhi ayinazithintelo. Flip iswitsha yamandla ngasemva ekhohlo kwirobhothi. Xa ivuliwe, i-gantry iya kwindawo yayo yasekhaya kwaye isikrini sokuchukumisa sibonisa imiyalelo eyongezelelweyo yoqwalaselo.

Ngoku ukuba iFlex yakho ingaphandle kwebhokisi kwaye ilungele ukuhamba, qhubela phambili kwicandelo lokuQala lokuQala elingezantsi.
2.3 Ukubaleka okokuqala
Yenza useto olusisiseko kwiscreen sokuchukumisa phambi kokuqhagamshela nayiphi na enye ihardware kwiFlex yakho. Irobhothi iya kukukhokela ngokuqhagamshela kwinethiwekhi yakho yelebhu, ukuhlaziya isoftware yamva nje, kunye nokwenza iFlex ibe yeyakho ngokuyinika igama.
Vula
Xa uvula iFlex, ilogo yeOpentrons iya kuvela kwiscreen esichukumisayo. Emva kwemizuzwana embalwa, iya kubonisa isikrini esithi "Wamkelekile kwi-Opentrons Flex yakho".
Isikrini sokwamkela i-Opentrons Flex. Kuya kufuneka ubone esi sikrini kuphela xa uqala iFlex yakho okokuqala.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
Qhagamshela kwinethiwekhi okanye ikhompyuter
Landela izikhokelo kwiscreen sokuchukumisa ukuze irobhothi yakho iqhagamshelwe ukuze ikwazi ukujonga uhlaziyo lwesoftware kwaye ifumane iprotocol files. Kukho iindlela ezintathu zokunxibelelana: iWi-Fi, i-Ethernet, kunye ne-USB.

Iinketho zonxibelelwano lwenethiwekhi. Kufuneka ube noqhagamshelo lwe-intanethi ukuseta iFlex. I-Wi-Fi: Sebenzisa isikrini sokucofa ukuze uqhagamshele kuthungelwano lwe-Wi-Fi olukhuselwe nge-WPA2 yoBuqinisekiso boBuqu (uninzi lweenethiwekhi ezifuna kuphela igama lokugqitha ukuze zidibanise ziwela phantsi kolu didi).
Qaphela: I-Flex ayizixhasi iiphothali ezibanjwayo (uthungelwano olungenalo igama lokugqitha kodwa lulayisha a webiphepha lokuqinisekisa abasebenzisi emva kokudibanisa).
Unako kwakhona ukuqhagamshela kwinethiwekhi ye-Wi-Fi evulekileyo, kodwa oku akukhuthazwa.
Isilumkiso: Ukuqhagamshela kwinethiwekhi ye-Wi-Fi evulekileyo kuya kuvumela nabani na okuluhlu lwesignali yenethiwekhi ukuba alawule irobhothi yakho ye-Opentrons Flex ngaphandle koqinisekiso.

Ukuba ufuna ukuqhagamshela kwinethiwekhi ye-Wi-Fi esebenzisa ukuqinisekiswa kweshishini (kuquka "i-eduroam" kunye nothungelwano lwezemfundo olufanayo olufuna igama lomsebenzisi kunye negama lokugqitha), qala ngokuqhagamshela kwi-Opentrons App nge-Ethernet okanye i-USB ukugqiba ukuseta kokuqala. Emva koko uqhagamshele kwinethiwekhi ye-Wi-Fi yeshishini kwiisetingi zothungelwano zeFlex yakho. Ukufikelela kwiisetingi zothungelwano:

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
1. Cofa izixhobo kwibar esecaleni esekhohlo ye-Opentrons App. 2. Cofa imenyu enamanqaku amathathu () kwiFlex yakho kwaye ukhethe Useto lweRobhothi. 3. Cofa i Uthungelwano thebhu.
Khetha inethiwekhi yakho kwimenyu eyehlayo okanye ukhethe "Joyina enye inethiwekhi..." kwaye ufake iSSID yayo. Khetha indlela yoqinisekiso lweshishini esetyenziswa yinethiwekhi yakho. Iindlela ezixhaswayo zezi:
I-EAP-TTLS ene-TLS EAP-TTLS ene-MS-CHAP v2 EAP-TTLS ene-MD5 EAP-PEAP ene-MS-CHAP v2 EAP-TLS
Nganye kwezi ndlela ifuna igama lomsebenzisi kunye negama lokugqitha, kwaye ngokuxhomekeke kuqwalaselo oluchanekileyo lwenethiwekhi yakho inokufuna isatifikethi files okanye ezinye iinketho. Qhagamshelana namaxwebhu e-IT kwiziko lakho okanye uqhagamshelane nomphathi wakho we-IT ngeenkcukacha zokuseta inethiwekhi yakho.
I-Ethernet: Qhagamshela irobhothi yakho kwisitshixo sothungelwano okanye ihabhu ngentambo ye-Ethernet. Ungaqhagamshela ngqo kwizibuko le-Ethernet kwikhompyuter yakho, ukuqala kwirobhothi yenguqulo 7.1.0.
I-USB: Qhagamshela intambo ye-USB A-to-B enikiweyo kwizibuko le-USB-B yerobhothi kunye nezibuko elivuliweyo kwikhompyutha yakho. Sebenzisa intambo ye-USB B-to-C okanye iadaptha ye-USB A-to-C ukuba ikhompuyutha yakho ayinayo i-USB-A port.
Ukuqhubeka nokuseta, ikhompyutha eqhagamshelweyo kufuneka ibe ne-Opentrons App efakiweyo kwaye iyasebenza. Ngeenkcukacha zokuhlohla i-Opentrons App, bona icandelo loFakelo lwe-App yesahluko seSoftware kunye nokuSebenza.
Faka uhlaziyo lwesoftware
Ngoku ekubeni uqhagamshelwe kwinethiwekhi okanye kwikhompyuter, irobhothi inokukhangela isoftware kunye nohlaziyo lwefirmware kwaye uzikhuphele ukuba kuyimfuneko. Ukuba kukho uhlaziyo, kungathatha imizuzu embalwa ukuyifaka. Nje ukuba uhlaziyo lugqityiwe, irobhothi iya kuqalisa kwakhona.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
Qhoboshela i-Emergency Stop Pendant
Qhagamshela i-Emergency Stop Pendant efakiweyo (E-stop) kwizibuko elincedisayo (AUX-1 okanye i-AUX-2) ngasemva kwirobhothi.

Ngaphambi nasemva kokudibanisa i-Emergency Stop Pendant.
Ukuqhoboshela kunye nokwenza ukuba i-E-stop ikwazi ukuncamathela izixhobo kunye nokusebenzisa iiprothokholi kwiFlex. Ngolwazi oluthe kratya malunga nokusebenzisa i-E-stop ngexesha lokusebenza kwerobhothi, jonga icandelo le-Emergency Stop Pendant yesahluko seNgcaciso yeNkqubo.
Nika irobhothi yakho igama
Ukuthiya irobhothi yakho kukuvumela ukuba uyichonge ngokulula kwindawo yakho yelebhu. Ukuba uneerobhothi ezininzi ze-Opentrons kumsebenzi womnatha wakho, qiniseka ukuba ubanika amagama awodwa. Nje ukuba uqinisekise igama lerobhothi yakho, uya kuthathwa kwiDashboard yakho ye-Opentrons Flex. Mhlawumbi inyathelo elilandelayo oya kufuna ukulithabatha kukuncamathisela izixhobo, ezigutyungelwe kwicandelo elilandelayo.
2.4 Ufakelo lwesixhobo kunye nolungelelwaniso
Emva kokuseta irobhothi yokuqala, inyathelo elilandelayo kukuncamathisela izixhobo kwirobhothi kwaye uzilinganise.
Ukufakela isixhobo, cofa kuqala kwi-Instruments kwiscreen esichukumisayo okanye uye kwicandelo lePipettes kunye neeModyuli zesikrini seenkcukacha zesixhobo kwi-Opentrons App. Khetha intaba engenanto kwaye ukhethe nokuba Faka iPipette okanye Qhoboshela iGripper. Ukuba intaba ofuna ukuyisebenzisa sele ihleli, kufuneka ukhuphe i-pipette okanye i-gripper kuqala.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
Qaphela: Inkqubo yokuhlohla iyonke iyafana nokuba usebenzisa isikrini esichukumisayo okanye i-Opentrons App. Nasiphi na isixhobo oqala kuso siya kulawula inkqubo yokuhlohla de ube uyigqibe okanye uyirhoxise.
Ukuba uqala kwiscreen esichukumisayo, usetyenziso luya kubonisa irobhothi “njengexakekile”. Ukuba uqala kwi-app, isikrini esichukumisayo siya kubonisa imodal ebonisa ukuba ukufakela isixhobo kuyaqhubeka.
Inkqubo yofakelo echanekileyo iyahluka ngokuxhomekeke kwisixhobo osiqhoboshelayo, njengoko kugutyungelwe kumacandelo angezantsi. Zonke izixhobo zinenkqubo yokulinganisa ngokuzenzekelayo, omele uyenze ngokukhawuleza emva kokufakwa.
Ukufakwa kwePipette
Xa ufaka i-pipette, uya kukhokelwa ngamanyathelo alandelayo kwisikrini sokuchukumisa okanye kwi-Opentrons App.
1. KHETHA UHLOBO LWEPIPETTE Khetha phakathi kwe-1- okanye i-8-Channel Pipette kunye ne-96-Channel Pipette. Ukuqhoboshela i-96-Channel Pipette ifuna amanyathelo ongezelelweyo ambalwa ngenxa yokuba incamathela kwipleyiti ekhethekileyo yokukhwela ejikeleza zombini ii-pipette mounts.
2. LUNGISELELA UKUFAKWA Susa ilebhu kwidekhi kwaye ucoce indawo yokusebenza ukwenza uncamathiselo kunye nokulinganisa kube lula. Kananjalo qokelela izixhobo ezifunekayo, ezifana necalibration probe, hex screwdriver, kunye nepleyiti yokunyuswa (ye-96-Channel Pipette).
3. QHAGAMSHELANA KWAYE UKHUSELE I-PIPETTE I-gantry iya kuhamba phambi kwerobhothi ukuze uqhoboshele i-pipette.
I-1- kunye ne-8-Channel Pipettes idibanisa ngokuthe ngqo kwi-pipette mount. I-96-Channel Pipette ifuna ipleyiti yokukhwela. Ukuze uqhoboshele ipleyiti yokunyusela, kufuneka uqale uqhawule uqhagamshelo lwe-z-axis kwindawo efanelekileyo yokunyusela ipayipi.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
Xhuma i-pipette kwi-pipette ekhethiweyo kwaye ukhusele izikrini zayo.
4. QAPHELA UKULUNGISWA OKUFANELEKILEYO Ukulinganisa i-pipette, faka i-probe yokulinganisa kwi-pipette nozzle efanelekileyo. I-pipette iya kuhamba ngokuzenzekelayo ukuchukumisa amanqaku athile kwidekhi kwaye igcine la maxabiso okulinganisa ukusetyenziswa kwexesha elizayo. Emva kokuba ulungelelwaniso lugqityiwe kwaye ususe i-probe, i-pipette iya kuba ilungele ukusetyenziswa kwiiprothokholi.
Ufakelo lweGripper
Xa ufaka i-gripper, uya kukhokelwa kula manyathelo alandelayo kwiscreen sokuchukumisa okanye kwi-Opentrons App.
1. LUNGISELELA UKUFAKWA Susa ilebhu kwidekhi kwaye ucoce indawo yokusebenza ukwenza uncamathiselo kunye nokulinganisa kube lula. Kwakhona qokelela i-screwdriver ye-hex efunekayo kwaye uqinisekise ukuba i-pin yokulinganisa isendaweni yayo yokugcina kwi-gripper.
2. QHAGAMSHELANA KWAYE UKHUSELE I-GRIPPER I-gantry iya kuhambela phambili kwirobhothi ukuze uqhoboshele into yokubamba. Xhuma i-gripper kwindawo yokwandisa kwaye ukhusele izikrufu zayo.
3. QAPHELA UKULUNGISA OKUZIQHELEKILEYO Ukulinganisa isibambi, faka ipini yokulinganisa kumhlathi ongaphambili. Isibambi siya ngokuzenzekelayo sichukumise amanqaku athile kumgangatho kwaye sigcine la maxabiso olungelelwaniso ukuze asetyenziswe kwixesha elizayo. Emva koko phinda inkqubo efanayo kunye nepini yokulinganisa kumhlathi ongasemva. Nje ukuba ulungelelwaniso lugqityiwe kwaye ubuyisele iphini kwindawo yayo yokugcina, igripper iya kuba ilungele ukusetyenziswa kwiiprothokholi.
2.5 Ukufuduswa kwenye indawo
Eli candelo libonelela ngeengcebiso kunye nemiyalelo malunga nendlela yokuhambisa irobhothi yakho ye-Opentrons Flex kumgama omfutshane kunye omde.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
Iintshukumo ezimfutshane
Intshukumo emfutshane ithatha uluhlu lwemigama ukusuka “masiyihambise kancinane” ukuya ngaphaya kwelebhu, phantsi kweholo, okanye omnye umgangatho kwisakhiwo sakho. Kwezi meko, ungahambisa iFlex yakho ngesandla. Ukuyihambisa kwinqwelo yesandla nayo yindlela efanelekileyo.
Isilumkiso: I-Flex inobunzima obungama-88.5 kg. Ngenxa yoko, kufuna abantu ababini ukuba bayiphakamise kwaye bayihambise ngokukhuselekileyo.
Qhoboshela kwakhona izibambo zokuphakamisa ukuhambisa iFlex yakho kwindawo entsha, ekufutshane. Ukuphakamisa nokuthwala iFlex ngezibambo zayo yindlela efanelekileyo yokuhambisa irobhothi imigama emifutshane. Susa izibambo kwaye uzigcine kwiKit yomsebenzisi emva kokuba intshukumo igqityiwe. Ukuthintela ukonakalisa irobhothi, soloko usebenzisa iziphatho zokuphakamisa ukuyichola nokuyihambisa. Sukubamba isakhelo ukuphakamisa okanye ukuhambisa irobhothi yakho.
Iintshukumo zomgama omde
Ukuhamba umgama omde kuthutha iFlex yakho kumhlaba weyunivesithi, indawo, okanye iziko. Kwidolophu ephela, ukuya kwisixeko esitsha, iphondo, iphondo, okanye ilizwe zonke zi-exampintshukumo yomgama omde. Kule meko, kuya kufuneka upakishe iFlex ukuyikhusela kwizinto ezothusayo, kunye neentshukumo ezirhabaxa ezinokuthi zenzeke ngelixa uhamba.
Ukuba uyigcinile ikhreyithi yokuthumela kunye nenkxaso yangaphakathi eze neFlex yakho, ungayipakisha kwakhona kwezi zixhobo zohambo lomgama omde. Landela amanyathelo e-unboxing ngokulandelelana ukuze ulungiselele iFlex yakho yokuhamba umgama omde. Ngokubanzi, kufuneka:
Susa umbane kunye nentambo yenethiwekhi, ukuba iqhotyoshelwe. Susa zonke izixhobo ezincanyathiselweyo kunye nelebhu. Qhoboshela iipleyiti zedekhi. Tshixa i-gantry (jonga icandelo leNgcebiso yokuHamba Jikelele ngezantsi). Susa kwaye ugcine iipaneli zefestile.
Ukuba ugcine ikhreyithi yoqobo:
Phinda unamathisele isakhelo sokuthumela kwi-Flex kwaye uyikhusele kwisiseko se-pallet usebenzisa i-L-brackets. Yongeza i-padding kwaye udibanise kwakhona ikhreyithi yokuthumela.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
Ukuba awunalo ikhreyithi yokuqala kunye nezinto ezinxulumeneyo, qhagamshelana nenkampani ethembekileyo yokuthumela ngenqanawa. Banokulawula ukupakisha, ukuthutha, kunye nenkqubo yokuhambisa kuwe.
Iingcebiso ezihambayo ngokubanzi
HLANGANISA AMANDLA NEENCAWU ZENEWEKHI Ngaphambi kokuba uhambise iFlex yakho, ungalibali: Cima umbane kwaye uwukhuphe kumbane. Nqamula i-Ethernet okanye intambo ye-USB, ukuba isetyenzisiwe.
TSHIXA IGANTRI Ngaphambi kokuba uhambise iFlex yakho, phinda ufake izikrufu zokutshixa ukuze ubambe igantry endaweni. Iindawo zokutshixa i-gantry zifumaneka: Kwicala lasekhohlo likaloliwe kufutshane nomphambili werobhothi. Ngaphantsi kwengalo ethe nkqo ye-gantry. Kwicala lasekunene likaloliwe kufutshane nomphambili werobhothi. Ukutshixa le nxalenye ye-gantry ifuna encinci
isibiyeli esiorenji kunye nezikrufu ezibini zokutshixa.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
IKHAYA IGANTRI Usenokungafuni ukutshixa i-gantry ukuba uhambisa irobhothi kwindawo ekufutshane. Ukuba uthatha isigqibo sokungayitshixi, ubuncinane sebenzisa isikrini sokuchukumisa okanye i-Opentrons App ukuthumela i-gantry kwindawo yayo yasekhaya phambi kokuba uyivule. Ukuya ekhaya i-gantry ngesikrini sokuchukumisa, cofa imenyu enamachaphaza amathathu () uze ucofe uKhaya gantry. Ukuya ekhaya i-gantry nge-Opentrons App: Cofa izixhobo. Cofa kwiFlex yakho kuluhlu lwesixhobo. Cofa imenyu enamachaphaza amathathu () uze ucofe uKhaya gantry.
SUSA IIMODULI ezikwidekhi kunye nezinye izincamatheliso zongeza ubunzima obongezelelweyo kwiFlex yakho. Zikwachaphazela umbindi werobhothi womxhuzulane, nto leyo enokwenza ukuba ive “incanyana” xa iyiphakamisa. Ukunceda ukukhanyisa kunye nokulinganisela irobhothi, susa naziphi na izixhobo ezincanyathiselwe kunye nelebhu ngaphambi kokuba uyichole.
BUYISE IZILOTHI ZOKUBUYISA Sicebisa ukuba uphinde udibanise iindawo zokubeka kwindawo yokubeka umgama omde. Ukukhusela iindawo zokubeka kwiindawo zabo zokuqala kunceda ukuthintela ilahleko ngengozi. Ukufakela kwakhona iindawo zokubeka idekhi kwiintshukumo ezimfutshane ezijikeleze ilebhu yinto yokuzikhethela.
ULUNGISELELO LWEEMVA KOKUSUKA Kufuneka uhlengahlengise naziphi na izixhobo kunye neemodyuli emva kokuba uzifakile kwakhona. Ukufumana iinkcukacha ezithe vetshe ngolungelelwaniso lwemodyuli, jonga iiModyuli isahluko.

I-OPENTRONS FLEX

ISAHLUKO SESI-2: UKUFAKELWA NOKUTSHINTSHA
Iingcamango zokugqibela malunga nokuhamba
I-Flex yakho ngumatshini owomeleleyo nowakhiwe kakuhle, kodwa ikwasisixhobo esichanekileyo sezenzululwazi esenzelwe ukunyamezela ukunyamezelana. Ngenxa yoko, kufuneka uyiphathe ngononophelo xa uyifudusela kwindawo osebenza kuyo okanye uyithumela kwilizwe lonke. Oku kuthetha ukulandela isikhokelo esinikelwe apha kunye nokusebenzisa eyakho ingqiqo malunga nendlela yokuthutha isixhobo selabhoratri esinexabiso eliphezulu. Umgca ophantsi: xa uhambisa i-Flex yakho, phutha kwicala lokulumkisa kunye ne-padding eyongezelelweyo.
Ukuba unemibuzo okanye iinkxalabo malunga nokufudusa iFlex yakho, qhagamshelana nathi ku support@opentrons.com.

I-OPENTRONS FLEX

ISAHLUKO 3
Inkcazo yeNkqubo

Esi sahluko sichaza iinkqubo ze-hardware ze-Opentrons Flex, eziphantsi kwe-core lab automation features. Umgangatho, i-gantry, kunye nezixhobo zokunyuswa kwe-Opentrons Flex yenza ukuba kusetyenziswe izinto ezichanekileyo zolwelo kunye ne-labware-handling. Isikrini sokuchukumisa esikwisixhobo senza ukuba kusebenze iiprothokholi kunye nokujonga ubume berobhothi ngaphandle kokuzisa ikhompyuter yakho kwibhentshi yelebhu. Uqhagamshelo oluneentambo nolungenazingcingo lwenza ulawulo olongezelelweyo oluvela kwi-Opentrons App (bona iSoftware kunye nesahluko sokuSebenza ngeenkcukacha ezithe vetshe) kunye nokwandisa iimpawu zenkqubo ngokuncamathisela iiperipherals (bona iiModyuli isahluko).

3.1 Amalungu omzimba

Ikhamera

Ukukhanya kwesimo

Isikrini esibanjwayo

Isakhelo

Idekhi yeGantry
Icango langaphambili
Iindawo zamalungu omzimba we-Opentrons Flex.

Ecaleni Windows Handle Caps

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO

Isakhelo kunye nendawo ebiyelweyo
Isakhelo serobhothi ye-Opentrons Flex ibonelela ngokuqina kunye nenkxaso yesakhiwo kwidekhi kunye ne-gantry. Zonke ii-subsystems zoomatshini zikwindawo kwaye zixhonywe kwisakhelo esingundoqo. Isakhelo sakhiwe ngokuyinhloko kwiphepha lesinyithi kunye ne-aluminium extrusions.
Isakhelo sesinyithi sinemingxuma yeefestile ezisecaleni kunye nomnyango wangaphambili owenziwe ngepolycarbonate ecacileyo ekuvumela ukuba ubone okwenzekayo ngaphakathi kweFlex. Iihenjisi zocango lwangaphambili zivuleleke ukufikelela ngaphakathi kwenkqubo. Ngomnyango wangaphambili ovulekileyo, unokuqhoboshela izixhobo, iimodyuli, kunye nokulungiswa kwedekhi; lungisa umgangatho phambi kweprothokholi; okanye ulawule ubume bomgangatho ngexesha leprotocol.
Imicu ye-LED emhlophe kwimiphetho ephezulu yesakhelo ibonelela ngokukhanya okulawulekayo kwesoftware. Ikhamera ye-2-megapixel inokufota umgangatho kunye nendawo yokusebenza yokurekhoda kunye nokulandela umkhondo wokwenziwa kweprotocol.

Idekhi kunye nendawo yokusebenza
Umgangatho ngumgangatho wealuminiyam eyenziwe ngomatshini apho iiprothokholi zesayensi ezizenzekelayo ziphunyezwa. Idekhi ineendawo zokubeka ezingundoqo ezili-12 ze-ANSI/SLAS-format ezinokuphinda ziqwalaselwe ukuze zibambe i-labware, iimodyuli, kunye nezinto ezisetyenziswayo. Iindawo zokubeka idekhi zichongwa yinkqubo yolungelelwaniso, kunye ne-slot A1 ngasemva ngasekhohlo kunye ne-D3 ekunene ngaphambili.

Slot Ukwandiswa (for Thermocycler) Indawo yokusebenza

StagIngingqi

Iindawo zedekhi ngaphakathi kweFlex.

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO
Indawo yokusebenza yindawo ebonakalayo ngaphezu kwedekhi efikelelekayo kwipayipi. Ilebhu ebekwe kwiindawo zokubeka i-A1 nge-D3 zikwindawo yokusebenza.
I-Opentrons Flex iza neendawo zokubeka ezisusekayo kuzo zonke izithuba ezili-12 kwindawo yokusebenza. Indawo nganye yokubeka idekhi ineekliphu zelebhu yekona yokubeka ngokukhuselekileyo i-labware kumgangatho.
Ungayiqwalasela kwakhona idesika ngokutshintsha iindawo zokubeka kunye nezinye izilungiso zedekhi, kubandakanya inkunkuma eshukumayo, i-chute yenkunkuma, kunye neemodyuli zemodyuli. I-slot yokwandisa emva kwe-A1 isetyenziselwa kuphela ukwenza igumbi elongezelelweyo kwiModyuli ye-Thermocycler, ehlala kwiindawo zokubeka i-A1 kunye ne-B1.
Qaphela: Iindawo zokubeka iDekhi ziyatshintsheka ngaphakathi kwikholamu (1, 2, okanye 3) kodwa hayi kwiikholam zonke; ikholamu 1 kunye nekholam yesi-3 iindawo zokubeka ziziqwenga ezahlukileyo nangona ubukhulu bazo obufanayo. Ungatsho ukuba yeyiphi ikholamu i-slot engena ngokuqhelanisa ikliphu ye-labware eluhlaza ngasemva ngasekhohlo.
Kuya kufuneka ushiye iindawo zokubeka idekhi ezifakwe kwiindawo apho ufuna ukubeka ilebhu ezimeleyo. Idekhi kunye nezinto ezibekwe kuyo zihlala zimile, ngaphandle kokuba zishukunyiswe yi-gripper okanye ukungenelela ngesandla.
Stagindawo
I stagindawo ye-ing yindawo eyongezelelweyo kwicala lasekunene lomgangatho. Uyakwazi ukugcina ilebhu kule ndawo emva kokufaka stagiindawo zokubeka. Ilebhu ibekwe kwiindawo zokubeka A4 nge D4 kwi stagindawo. I-Flex pipettes ayikwazi ukufikelela kwi-stagIndawo, kodwa into yokubamba inokuthatha kwaye ihambise ilebhu ukuya nokusuka kule ndawo. Ukongeza iindawo zokubeka ezongezelelweyo kunceda ukugcina indawo yokusebenza ifumaneka kwisixhobo esisetyenziswa kwiiprothokholi zakho ezizenzekelayo.
Stagiindawo zokubeka iindawo zokubeka zibandakanyiwe kuqwalaselo oluthile lwendawo yokusebenza kwaye zikwafumaneka ukuba zithengwe kwi-https://shop.opentrons.com.

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO

Staging Indawo kunye Slots ezifakiweyo

Izixhobo zedesika
Ukulungiswa zizinto zehardware ezithatha indawo yeendawo zokubeka ezisemgangathweni. Bakuvumela ukuba ulungise uyilo lwedekhi kwaye wongeze ukusebenza kwiFlex yakho. Okwangoku, ukulungiswa kwedesika kubandakanya i-stagiindawo zokubeka iindawo zokubeka, umgqomo wenkunkuma wangaphakathi, kunye ne-chute yenkunkuma yangaphandle. Ungafakela kuphela izixhobo kwiindawo zokubeka ezimbalwa. Le theyibhile ilandelayo idwelisa iindawo zomgangatho womdlalo ngamnye.

Ulungiso Staging indawo kwiindawo zokubeka Inkunkuma umgqomo weNkunkuma chute Inkunkuma chute nge stagindawo slot

Slots A3D3 A1D1 kunye A3-D3 D3 kuphela D3 kuphela

Ulungiso alunamandla. Aziqulathanga izinto zombane okanye ezomatshini ezinxibelelana nemeko yazo yangoku kunye nendawo yedekhi kwirobhothi. Oku kuthetha ukuba kufuneka usebenzise inqaku lokumisela idekhi ukuze uvumele iFlex yazi ukuba zeziphi izinto ezincanyathiselwe kumgangatho kunye nalapho zikhoyo.

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO

Ungafikelela kwisethingi yoqwalaselo lwedekhi ukusuka kwiscreen sokuchukumisa usebenzisa amachaphaza amathathu () menu nakwiOpentrons App. Jonga icandelo loLungiselelo lweDekhi yeSoftware kunye nesahluko sokuSebenza ngolwazi malunga nendlela yokuqwalasela umgangatho ukusuka kwiscreen sokuchukumisa.

I-chute yenkunkuma
I-Opentrons Flex Waste Chute ihambisa ulwelo, iingcebiso, ii-tip racks, kunye neepleyiti zamaqula ukusuka kwindawo ebiyelweyo yeFlex ukuya kwindawo yokulahla inkunkuma ebekwe ngaphantsi kokuvulwa kwayo kwangaphandle. I-chute yenkunkuma incamathela kwi-adaptha yepleyiti ekwidekhi elingana ne-slot D3. Ikwaza nefestile ekhethekileyo yesiqingatha sephaneli evumela i-chute ukuba iphumele ngaphandle kwerobhothi.
Amalungu echute yenkunkuma.

I-Adapter yepleyiti yeNgqungquthela

Inkunkuma Chute
I-Adapter yepleyiti yeDekhi ene-StagIngingqi

Stagiindawo zokubeka
Stagiindawo zokubeka iindawo zokubeka ziyi-ANSI/SLAS ezihambelanayo zedekhi iziqwenga ezithatha indawo yokubeka umgangatho kwikholamu yesi-3 kwaye yongeze iindawo zokubeka ezintsha kwi-s.tagindawo - konke ngaphandle kokuphulukana nendawo kwindawo yokusebenza. Ungafakela indawo yokubeka enye okanye ubuninzi beendawo zokubeka ezine ukwenza ikholamu entsha (A4 ukuya kwi-D4) kwicala lasekunene ledekhi. Qaphela, nangona kunjalo, ukuba indawo ye-deck slot A3 ifuna ukuhambisa umgqomo wenkunkuma. Ngokudibanisa u-stagkwiindawo zokubeka kwiindawo zokubeka, irobhothi yakho yeFlex inokugcina i-labware eninzi kwaye isebenze ngokufanelekileyo.

I-OPENTRONS FLEX

Flex stagindawo slot.
49

ISAHLUKO SE-3: INKCAZELO YENKQUBO
UKUFAKWA KWESlot
Ukufakela, susa izikrufu eziqhoboshela i-slot esemgangathweni kwidekhi kwaye uyibuyisele nge-stagindawo slot. Emva kofakelo, sebenzisa isikrini sokuchukumisa okanye i-Opentrons App ukuxelela irobhothi oyongeze ngayotagindawo yokubeka kwindawo yokupaka.
Ukuhlohla njengetagindawo slot.

UKUHLANGABEZANA KWESIBALA Stagiindawo zokubeka kwiindawo zokubeka iyahambelana izixhobo Flex, iimodyuli, kunye labware ezidweliswe ngezantsi.

Flex component Gripper Pipettes Modules
Ilebhu

Stagukuhambelana kwendawo
I-Flex Gripper inokuhambisa i-labware ukuya okanye ukusuka kwi-stagiindawo zokubeka.
I-Flex pipettes ayikwazi ukufikelela kwi-stagindawo. Sebenzisa into yokubamba ukuhambisa incam yeeracks kunye ne-labware ukusuka kwi-stagindawo yokusebenzela kwindawo yokusebenzela ngaphambi kokuba umbhobho.
Ibhloko yeMagnetic GEN1 inokubekwa kwikholam yesi-3 ngaphezulu kwetagindawo slot. Iimodyuli azixhaswanga kuluhlu lwesi-4.
Iimodyuli ezinamandla ezifana ne-Heater-Shaker kunye neModyuli yoBubushushu zingena kwiikhadi ezinokubekwa kwikholam yesi-3. Awunako ukongeza njengetagindawo yokubeka indawo kwindawo ehlala imodyuli yecaddy.
Stagiindawo zokubeka iindawo zokubeka zinemilinganiselo efanayo ye-ANSI/SLAS njengeendawo zokubeka ezisemgangathweni. Sebenzisa i-labware ehambelana ne-gripper kwi-stagindawo, okanye yongeza ngesandla kwaye ususe ilebhu kule ndawo.

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO
Inkqubo yentshukumo
Incanyathiselwe kwisakhelo yigantry, eyintshukumo yerobhothi kunye nenkqubo yokubeka. I-gantry ihamba ngokwahlukeneyo ecaleni kwe-x- kunye ne-y-axis ukubeka iipayipi kunye ne-gripper kwiindawo ezichanekileyo zokwenziwa kweprotocol. Ukushukuma ecaleni kwala mazembe kuchanekile ukuya kweyona ikufutshane yi-0.1 mm. I-gantry ilawulwa yi-36 VDC hybrid bipolar stepper motors. Ngaloo ndlela, iqhotyoshelwe kwi-gantry i-pipette mounts kunye ne-extension mount. Ezi zihamba ecaleni kwe-z-axis ukubeka iipayipi kunye ne-gripper kwiindawo ezichanekileyo zokwenziwa kweprotocol. Ukushukuma kule axis kulawulwa 36 VDC hybrid bipolar stepper motors. I-electronics equlethwe kwi-gantry inikezela amandla e-VDC angama-36 kunye nonxibelelwano kwiipayipi kunye ne-gripper, xa iqhotyoshelweyo.
Gantry

Pipette Mounts

INtaba Yokwandiswa

Indawo yokuxhoma isixhobo kwiFlex.

Isikrini esichukumisayo kunye neziboniso ze-LED
Ujongano oluphambili lomsebenzisi yi-7-intshi ye-LCD yescreen sokuchukumisa, esiphambi kwerobhothi ekunene. Isikrini sokuchukumisa sigqunywe ngeGorilla Glass 3 yokukrweleka kunye nokumelana nomonakalo. Fikelela kwiimpawu ezininzi zeFlex ekunene kwiscreen esichukumisayo, kubandakanya:

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO

Ulawulo lweProtocol Ukuseta iProtocol, ukuphunyezwa, kunye nokubek'esweni Ulawulo lweLabware Useto lweRobhothi Inkqubo yesoftware kunye nohlaziyo lwefirmware Iilog zokusebenza kunye nezaziso zempazamo.
Ukufumana ulwazi oluthe kratya malunga nokusebenzisa i-Flex nge-touchscreen, jonga i-Touchscreen Operation icandelo le-Software kunye nesahluko sokuSebenza.
Isibane sesimo sisicu see-LED ecaleni komphambili werobhothi ebonelela ngolwazi lokujonga nje malunga nerobhothi. Imibala eyahlukeneyo kunye neepatheni zokukhanyisa zinokunxibelelana ngempumelelo, ukusilela, okanye imeko engasebenziyo:

Umbala we-LED oMhlophe ngokungathathi hlangothi
Green Normal states
Blue Mandatory ithi Yellow Abnormal states Red Emergency states

Ipateni ye-LED Ukutyhala okuqinileyo
Ukuqhwanyaza kabini
I-Solid Pulsing Pulsing

Imo yerobhothi
Ilayishiwe kwaye ayisebenzisi iprothokholi Irobhothi ixakekile (umzekelo, ukuhlaziya isoftwe okanye i-firmware, ukuseta iprotocol baleka, ukucinywa kweprotocol run) Isenzo sigqityiwe (umzekelo, iprotocol egciniweyo, isoftwe ihlaziywe, isixhobo esincanyathiselwe okanye sivaliwe) Iprothokholi iyasebenza iProtocol igqityiwe. Umgaqo unqunyanyisiwe

Iqinile

Impazamo yesoftware

Uqhwanyaza kathathu, ngokuphindaphindiweyo

Impazamo ebonakalayo (umzekelo, ukungqubana kwesixhobo)

Ukukhanya kwesimo kunokucinywa kwiisetingi zerobhothi.

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO
3.2 IiPipettes
I-Opentrons pipettes zizixhobo ezilungisekayo ezisetyenziselwa ukuhambisa ulwelo kuyo yonke indawo yokusebenza ngexesha lokwenziwa kweeprothokholi. Kukho iipipette ezininzi ze-Opentrons Flex, ezinokuphatha imiqulu ukusuka kwi-1 µL ukuya kwi-1000 µL kwisi-1, isi-8, okanye sama-96 amajelo:
I-Opentrons Flex 1-Channel Pipette (1 µL) Opentrons Flex 50-Channel Pipette (1 µL) Opentrons Flex 5-Channel Pipette (1000 µL) Opentrons Flex 8-Channel Pipette (1 µL) I-Opentrons 50 Flex 8x 5
Iipipettes zifakela kwi-gantry usebenzisa izikrufu ezithinjiweyo phambi kwepipette. I-1-channel kunye ne-8-channel pipettes nganye ithatha i-pipette mount enye (ekhohlo okanye ekunene); i-96-channel pipette ithatha zombini ii-mounts. Ukufumana iinkcukacha ngokufakela iipipettes, jonga ukuFakelo lweSixhobo kunye nokuKhawulwa.

Isiqhoboshelo esiqhotyoshelweyo
Izikrufu

Izikrufu zokuncamathisela ezithinjiweyo

Isixhobo

Imilomo (i-O-rings enokubuyiselwa)

Imilomo (i-O-rings esisigxina)

Iindawo zamacandelo e-1-, 8-, kunye ne-96-channel pipettes.

Isixhobo

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO

Iipipette zithatha iingcebiso zeplastiki ezilahlwayo ngokuzicinezela kwimibhobho ye-pipette, kwaye emva koko zisebenzisa iingcebiso zokunqwenela kunye nokukhupha ulwelo. Isixa samandla ewonke afunekayo ekuthatheni ayanda njengoko iingcebiso ezininzi zicholwa ngaxeshanye. Ngamanani amancinci amancinci, i-pipette idibanisa iingcebiso ngokutyhala i-pipette nozzle nganye phantsi kwi-tip. Ukufezekisa amandla afunekayo okuthatha i-rack epheleleyo yeengcebiso, i-pipette ye-channel 96 iphinda ikhuphe iingcebiso phezulu kwimibhobho. Esi senzo sokutsala sidinga ukubeka i-tip racks kwi-adaptha ye-tip rack, endaweni yokuba ngqo kwi-slot yedekhi. Ukulahla iingcebiso (okanye uzibuyisele kwi-rack yazo), i-pipette ejector mechanism ityhala iincam eziphuma kwimibhobho.

Iimpawu zePipette
Iipayipi ze-Opentrons Flex zenzelwe ukuphatha uluhlu olubanzi lwemiqulu. Ngenxa yoluhlu olubanzi ngokubanzi, banokusebenzisa ubungakanani obuninzi beengcebiso, ezichaphazela iimpawu zabo zokuphatha ulwelo. I-Opentrons ivavanye i-Flex pipettes yokuchaneka kunye nokuchaneka kwinani leengcebiso kunye nendibaniselwano yevolumu yolwelo:

Pipette
Flex 1-Channel
50µL
Flex 1-Channel
1000µL
Flex 8-Channel
50µL
Flex 8-Channel
1000µL

ITip Umthamo 50 µL 50 µL 50 µL 50 µL 50 µL 200 µL 1000 µL 50 µL 50 µL 50 µL 50 µL 50 µL 200L 1000

Uvavanyo lweVolume 1 µL 10 µL 50 µL 5 µL 50 µL
200µL 1000µL
1 µL 10 µL 50 µL 5 µL 50 µL 200 µL 1000 µL

Ukuchaneka %D 8.00% 1.50% 1.25% 5.00% 0.50% 0.50% 0.50% 10.00% 2.50% 1.25% 8.00% 2.50% 1.00% 0.70%

Ukuchaneka %CV 7.00% 0.50% 0.40% 2.50% 0.30% 0.15% 0.15% 8.00% 1.00% 0.60% 4.00% 0.60% 0.25% 0.15%

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO

Flex 96-Channel
1000µL

50 µL 50 µL 200 µL 1000µL

5 µL 50 µL 200 µL 1000µL

10.00% 2.50% 1.50% 1.50%

5.00% 1.25% 1.25% 1.50%

Gcina olu lwazi luchanekileyo engqondweni xa ukhetha iingcebiso kwi-pipette yakho. Ngokubanzi, iziphumo ezilungileyo kufuneka usebenzise amacebiso amancinci ahlangabezana neemfuno zeprotocol yakho.

Qaphela: I-Opentrons yenza uvavanyo lwe-volumetric ye-Flex pipettes ukuqinisekisa ukuba ziyahlangabezana nokuchaneka kunye neenkcukacha ezichanekileyo ezidweliswe ngasentla. Awudingi ukulinganisa umthamo okhutshwa ngeepipettes zakho ngaphambi kokusetyenziswa. Kufuneka wenze ulungelelwaniso lwendawo kuphela. Jonga icandelo elilandelayo, kunye necandelo loFakelo lwePipette kwisahluko sokuFakelo kunye nokuTshintshwa, ngeenkcukacha.
Iinkonzo ze-Opentrons Care kunye ne-Opentrons Care Plus zibandakanya ukutshintshwa kwepipette yonyaka kunye nezatifikethi zokulinganisa. Jonga icandelo leService Flex kwisahluko seSondlo neNkonzo ngeenkcukacha.

Ukulinganisa kwePipette
I-User Kit ibandakanya iprobe yokulinganisa i-pipette yensimbi, oyisebenzisayo ngexesha lokulinganisa indawo. Ngexesha lokuqhuba kweprotocol, gcina ngokukhuselekileyo iprobe kwisibambi semagnethi kwintsika yangaphambili yerobhothi. Ngethuba lenkqubo yokulinganisa, faka i-probe kwi-nozzle efanelekileyo kwaye uyitshixe endaweni. Irobhothi ihambisa iprobe kwindawo zokulinganisa kwidekhi ukulinganisa indawo ngqo yepipette.
Iadaptha ye-rack ye-Pipette
Iinqanawa ze-Opentrons Flex 96-channel pipette ezine-adaptha ezine-tip rack. Ezi zizibiyeli ezenziwe nge-aluminiyam ezichanekileyo ozibeke kumgangatho. Iiadaptha zibamba i-Flex 50 L, 200 L, kunye ne-1000 µL yeerekhi zeengcebiso.

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO
Ngenxa yamandla abandakanyekayo, i-pipette ye-96-channel idinga i-adapter ukuba ifake i-tip rack epheleleyo ngokufanelekileyo. Ngethuba lenkqubo yokuncamathisela, i-pipette ihamba phezu kwe-adapter, iyancipha ngokwayo kwizikhonkwane zokukhwela, kwaye idonsa iingcebiso kwiipayipi ngokuphakamisa i-adapter kunye ne-tip rack. Ukutsala iingcebiso, kunokuba utyhale, kunika amandla afunekayo ekukhuseleni iingcebiso kwiipipettes kunye nokuthintela ukulwa nomgangatho womgangatho. Xa igqityiwe, i-pipette ye-96-channel yehlisa iadaptha kunye ne-tip engenanto kwidekhi. Bona icandelo leeNgcebiso kunye neengcebiso zokubeka kwisahluko seLabware ngolwazi oluthe kratya.
Ukuthatha ingcebiso engaphelelanga
I-pipette ye-96-channel inokuthatha i-rack epheleleyo yeengcebiso okanye inani elincinci leengcebiso. Oku kwandisa inani lezicelo onokuthi uzenze nge-pipette ye-channel 96, kuba ithatha zombini ii-pipet mounts.
Okwangoku, i-pipette ye-96-channel isekela i-pickup yencam yencam yeengcebiso ezi-8 kuyilo lwekholamu. Kolu lungelelwaniso, i-pipette mhlawumbi isebenzisa imilomo yayo yasekhohlo ukuze ithathe iingcebiso ekunene ukuya ekhohlo ukusuka kwi-tip rack, okanye imilomo yayo ekunene ukuchola iingcebiso ukusuka ekhohlo ukuya ekunene kwi-tip rack.
Xa uthatha iingcebiso ezingaphantsi kwe-96 kwi-tip rack, i-rack kufuneka ibekwe ngokuthe ngqo kwi-deck, kungekhona kwi-adaptha ye-tip rack.
Iinzwa zePipette
I-Opentrons Flex pipettes inenani leenzwa ezifumanisa kwaye zirekhode idatha malunga nesimo se-pipette kunye naziphi na iingcebiso ezithathiweyo.
IZIVAMVA ZEZAKHONO
Ngokudibanisa neprobe yensimbi okanye i-tip conductive, i-capacitance sensors ibona xa i-pipette idibanisa nento ethile. Ukufunyanwa koqhagamshelwano phakathi kweprobe yensimbi kunye nedekhi isetyenziswe kwi-automated pipette calibration kunye neenkqubo zokulinganisa imodyuli.
I-pipettes ye-channel ye-1 ine-sensor ye-capacitance enye, ngelixa i-pipettes ye-channel inamabini amabini: kwi-channel 1 kunye ne-8 ye-pipettes ye-8-channel, kunye ne-channel 1 kunye ne-96 (izikhundla ze-A1 kunye ne-H12) ze-96-channel pipette.

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO

IZIMVO ZOKUBONA ICEBISO UBUKHONA
I-photointerruptor switch ibona indawo ye-pipette ye-tip ejector mechanism, iqinisekisa ukuba iingcebiso zicholwe ngempumelelo okanye ziwisiwe. I-1-channel, i-8-channel, kunye ne-96-channel pipettes zonke zine-sensor ye-optical enye ebeka esweni i-tip attachment kuwo onke amajelo.
Pipette firmware updates
I-Opentrons Flex ihlaziya ngokuzenzekelayo i-firmware ye-pipette ukuyigcina ihambelana nenguqulelo yesoftware yerobhothi. Uhlaziyo lwePipette firmware luhlala lukhawuleza, kwaye luyenzeka nanini na:
Uqhoboshela ipipette. Irobhothi iqala kwakhona.
Ukuba, ngenxa yaso nasiphi na isizathu, i-firmware yakho ye-pipette kunye ne-robot software versions ziphuma kwi-sync, unokuhlaziya ngesandla i-firmware kwi-Opentrons App.
1. Cofa izixhobo. 2. Cofa kwiFlex yakho kuluhlu lwesixhobo. 3. Ngaphantsi kweZixhobo kunye neeModyuli, i-pipette ephuma ngaphandle iya kubonisa ukufundwa kwebhena yesilumkiso
"Uhlaziyo lweFirmware luyafumaneka." Cofa uHlaziyo ngoku ukuqalisa uhlaziyo.
Unga view inguqulelo ye-firmware efakwe ngoku yayo nayiphi na ipayipi encanyathiselweyo. Kwisikrini esichukumisayo, yiya kwi-Instruments kwaye ucofe igama lepipette. Kwi-Opentrons App, fumana ikhadi le-pipette phantsi kwe-Instruments kunye neeModyuli, cofa imenyu yamachaphaza amathathu (), uze ucofe Malunga nepipette.

3.3 Igripper
I-gripper ihambisa i-labware kuyo yonke indawo yokusebenza kunye ne-stagindawo ngexesha lokwenziwa kweprotocol. I-gripper inamathele kwintaba yokwandiswa, eyahlukileyo kwi-pipette mounts; i-gripper ingasetyenziswa kunye naluphi na uqwalaselo lwepipette. Ukufumana iinkcukacha ngokufakela igripper, jonga uFakelo lweSixhobo kunye noLungiso.
Isibambi sinokuhambisa ilebhu ngapha kwedekhi kwaye ingene okanye ikhuphe iimodyuli. Isibambi sinokusebenzisa iipleyiti zequla ezidityanisiweyo ngokupheleleyo, iipleyiti zequla elinzulu, kunye neendawo zokubeka. Ukufumana iinkcukacha ezithe vetshe malunga nokuba yeyiphi ilebhu enokuthi ihambe, jonga iLabware kunye neOpentrons Flex Gripper icandelo lesahluko seLabware, okanye ujonge iLayibrari yeLabware yeOpentrons.

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO

Iimpawu zeGripper
Imihlathi yenza intshukumo ephambili ye-gripper, leyo kukuvula okanye ukuvala iipaddles ezimbini ezihambelanayo ukuze zifake okanye zikhulule amandla kumacala e-labware. Ukuhamba kwemihlathi kulawulwa yi-36 VDC ye-brush motor motor exhunywe kwi-rack-and-pinion gear system.
Ukuhambisa iqhekeza le-labware eliye labanjwa yimihlathi, i-gantry iphakamisa i-gripper ecaleni kwe-axis ye-z, iyihambisa ecaleni, kwaye iyihlisele kwindawo entsha ye-labware.
Iindawo zamacandelo e-gripper.

Izikrufu zokuncamathisela
Isikhonkwane sokulinganisa iiJaws Paddles

Ukulinganisa kweGripper
I-gripper iquka ipini yokulinganisa isinyithi. I-pin yokulinganisa ibekwe kwindawo yokugcina i-recessed kwindawo engezantsi ye-gripper. Umazibuthe ubamba iphini endaweni yalo. Ukususa ipini yokulinganisa, yibambe ngeminwe yakho kwaye uyitsale ngobunono. Ukutshintsha iphini, yibuyisele kwindawo yokugcina. Uya kuyazi ukuba ikhuselekile xa ingena endaweni.
Xa ulinganisa i-gripper, faka ipini kumhlathi ngamnye ngokulandelelana. Irobhothi ihambisa iphini kwiindawo zokulinganisa kwidekhi ukulinganisa eyona ndawo ikhoyo yokubamba.
Ngexesha lokuqhuba kweprotocol, beka ipini kwindawo yayo yokugcina ukuze ugcinwe ngokukhuselekileyo. Qhagamshelana nathi ku-support@opentrons.com ukuba uphulukene nepini yokulinganisa.

Gripper firmware updates
I-Opentrons Flex ihlaziya ngokuzenzekelayo i-firmware yegripper ukuyigcina ingqamanise nenguqulelo yesoftware yerobhothi. Uhlaziyo lwe-firmware yeGripper luhlala lukhawuleza, kwaye luyenzeka nanini na:

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO
Uqhoboshela into yokubamba. Irobhothi iqala kwakhona.
Ukuba, ngenxa yaso nasiphi na isizathu, i-firmware yakho yegripper kunye neenguqulelo zesoftware yerobhothi ziyaphuma ku-sync, ungenza ukuhlaziya i-firmware kwi-Opentrons App.
1. Cofa izixhobo. 2. Cofa kwiFlex yakho kuluhlu lwesixhobo. 3. Ngaphantsi kwezixhobo kunye neeModyuli, igripper engaphandle kwe-sync iya kubonisa ukufundwa kwebhena yesilumkiso.
"Uhlaziyo lweFirmware luyafumaneka." Cofa uHlaziyo ngoku ukuqalisa uhlaziyo.
Unga view inguqulelo yefirmware efakiweyo yangoku ye gripper. Kwisikrini esichukumisayo, yiya kwi-Instruments kwaye ucofe into yokubamba. Kwi-Opentrons App, fumana ikhadi lokubamba phantsi kwezixhobo kunye neeModyuli, cofa imenyu enamachaphaza amathathu (), uze ucofe Malunga nento yokubamba.
3.4 I-Emergency Stop Pendant
I-Emergency Stop Pendant (i-E-stop) yiqhosha le-hardware elizinikeleyo lokumisa ngokukhawuleza ukunyakaza kwerobhothi. I-Opentrons Flex ifuna i-E-stop esebenzayo, engasetyenziswanga ukuba incanyathiselwe ngawo onke amaxesha. Xa ucofa iqhosha lokumisa, iFlex irhoxisa nayiphi na iprotocol esebenzayo okanye ukuseta ukuhamba komsebenzi ngokukhawuleza kwaye inqanda uninzi lwentshukumo yerobhothi.
Kusetyenziswa nini i-E-stop
Unokufuna ukucofa i-E-stop:
Xa kukho umngcipheko wokwenzakala okanye ukwenzakala kumsebenzisi. Xa kukho ingozi esondeleyo yomonakalo kwirobhothi okanye enye i-hardware. Xa sampii-les okanye ii-reagents zisengozini esondeleyo yokosuleleka. Emva kokungqubana kwehardware.
Ngokufanelekileyo awufanelanga ukuba ucinezele i-E-stop (ngaphandle kwexesha lokuvavanywa komgangatho wehardware).
Musa ukusebenzisa i-E-stop ukurhoxisa imisebenzi eqhelekileyo, elindelekileyo. Endaweni yoko, sebenzisa iqhosha lesoftware kwiscreen sokuchukumisa okanye kwi-Opentrons App. Ukunqumamisa ngesoftware kuya kukuvumela ukuba uqalise okanye urhoxise umthetho olandelwayo, ngelixa ukucofa i-E-stop kusoloko kuyirhoxisa iprotocol ngoko nangoko.

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO

Ukuzibandakanya kunye nokukhulula i-E-stop
I-E-stop ine-cofa-to-engege, i-twist-to-release mechanism.
Zibandakanye: Cofa ezantsi ngokuqinileyo kwiqhosha elibomvu. I-Flex iya kungena kwimeko yokuyeka. Sombulula: Nje ukuba uyekile, lungisa ngokukhuselekileyo naziphi na iingxaki kwindawo yokusebenza, njengokucoca ukuchitheka,
ukususa i-labware, okanye ukuhambisa i-gantry (kufuneka ihambe ngokukhululekileyo kwaye ngokulula ngesandla). Khupha: Jija iqhosha ngokwewotshi. Iyakuvela kwindawo yayo yokungahlali. Seta kwakhona: Kwisikrini sokuchukumisa okanye kwi-Opentrons App, qinisekisa ukuba ukulungele ukuba iFlex iqalise kwakhona
intshukumo. I-gantry iya kubuyela kwindawo yayo yasekhaya kwaye umsebenzi wemodyuli uya kuqalisa kwakhona.
Kwimeko yokumisa, iFlex kunye nehardware edibeneyo iya kuziphatha ngolu hlobo lulandelayo:

Hardware Gantry Pipettes
Igripper
Imodyuli yesifudumezi-Shaker
Ubushushu beModyuli yeThermocycler Imodyuli yeModyuli ukukhanya kwescreen seTouchscreen

Ukuziphatha
Isindululo esizisebenzelayo esithe tyaba simisiwe. Ukunyakaza okuthe tyaba okubhalwe ngesandla kuvumelekile.
Ukuhamba ngokuthe nkqo kweepipettes kumisiwe. Iziqhoboshi zemoto kwii-axes ezithe nkqo zibotshelelwe ukuthintela
pipettes ekuweni. Intshukumo yePlunger kunye nokuthatha ingcebiso kunqunyanyisiwe.
Intshukumo ethe nkqo yesibambi imisiwe. I-motor brake kwi-axis ethe nkqo ibandakanyeka ukukhusela
into yokubamba ekuweni. Iinjini zemihlathi ezisebenzisa amandla okubamba zihlala zisebenza, ke
i-gripper ayiyi kuwisa i-labware enokuthi iphathe.
Ishaker iyayeka kwaye igoduke. Ilatshi yelebhu iyavula. Ukufudumeza kuvaliwe.
Ukufudumeza okanye ukupholisa kuvaliwe.
Ukufudumeza okanye ukupholisa kuvaliwe.
Ukukhanya kuba bomvu.
Umyalezo wokurhoxisa uthatha isikrini. Isalathisi sesikrini sibonisa xa uphumelele ngempumelelo
likhuphe iqhosha lokumisa.

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO
3.5 Uqhagamshelwano

Vula/Vala Tshintsha

Icala ukuvaleka

Izibuko ze-USB-A

I-IEC Power Inlet

Amazibuko AUX-1, AUX-2, USB-B, Ethernet

Uqhagamshelwano lwamandla
I-Opentrons Flex idibanisa kumthombo wamandla ngokusebenzisa i-IEC-C14 inlet eqhelekileyo. I-robot iqulethe i-internal full-range AC / DC power supply, eyamkela i-100 VAC, i-240/50 Hz igalelo kwaye iguqulelwe kwi-60 VDC. Zonke ezinye ii-electronics zangaphakathi zinikwa amandla yi-36 VDC supply.

Isilumkiso: Sebenzisa kuphela intambo yombane enikelwe nerobhothi. Musa ukusebenzisa intambo yamandla enomsinga onganelanga okanye ivolthitage iireyithingi.
Gcina intambo yombane ingenazithintelo ukuze ukwazi ukuyisusa ukuba kuyimfuneko.

Kukwakho ne-CR1220 yentsimbi yebhetri yeselula ukunika amandla iwotshi yexesha lokwenyani yerobhothi xa ingaqhagamshelwanga kumandla amakhulu. Ibhetri ibekwe ngaphakathi kwendawo ebiyelweyo yesikrini. Qhagamshelana neNkxaso ye-Opentrons ngolwazi oluthe kratya ukuba ucinga ukuba ufuna ukubuyisela ibhetri.

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO
I-USB kunye noqhagamshelwano oluncedisayo
I-Opentrons Flex ine-10 iyonke izibuko ze-USB ezibekwe kwiindawo ezahlukeneyo zerobhothi, ezisebenza ngeenjongo ezahlukeneyo.
Amazibuko asisi-8 angasemva e-USB-A (amanani e-USB-1 nge-USB-8) kunye namazibuko angama-2 ancedisayo (izihlanganisi ze-M12 ezinenombolo AUX-1 kunye ne-AUX-2) zezokudibanisa iimodyuli ze-Opentrons kunye nezixhobo. Jonga iiModyuli zesahluko ngolwazi oluthe kratya malunga nokudibanisa ezi zixhobo kunye nokuzisebenzisa kwiiprothokholi zakho. Izibuko le-USB-B elingasemva lelokudibanisa irobhothi kwilaptop okanye kwikhompyuter yedesktop, ukuseka unxibelelwano kunye ne-Opentrons App esebenza kwikhompyuter eqhagamshelweyo. I-USB-A port yangaphambili (USB-9), ibekwe ngezantsi kwesikrini sokuchukumisa, inomsebenzi ofanayo nomva we-USB-A.
Qaphela: Amazibuko e-USB anamandla alinganiselwe ukukhusela irobhothi kunye nezixhobo eziqhagamshelweyo. Unikezelo lwamandla lwahlulwe ngaphakathi ngokwamaqela amathathu ezibuko: izibuko le-USB-A lasekhohlo (USB-1 nge-USB-4), izibuko le-USB-A lasekunene (USB-5 nge-USB-8), kunye ne-USB-A yangaphambili. izibuko. Ngalinye lala maqela liya kuhambisa ubuninzi be-500 mA kwizixhobo ezihambelanayo ze-USB 2.0.
Uqhagamshelwano lwenethiwekhi
I-Opentrons Flex inokuqhagamshela kuthungelwano lwendawo yasekuhlaleni ngoqhagamshelwano olunentambo (Ethernet) okanye olungenazingcingo (Wi-Fi).
I-Ethernet port ibekwe ngasemva kwirobhothi. Qhagamshelana ne-Ethernet hub okanye utshintshe inethiwekhi yakho. Okanye, ukuqala kwi-robot system version 7.1.0, yiqhagamshele ngokuthe ngqo kwi-Ethernet port kwikhompyuter yakho. Imodyuli yangaphakathi ye-Wi-Fi ixhasa i-802.11 ac/a/b/g/n networks nge-dial-band 2.4/5 GHz antenna.

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ISAHLUKO SE-3: INKCAZELO YENKQUBO

3.6 Iinkcukacha zenkqubo

Iinkcukacha ngokubanzi

Imilinganiselo Ubunzima kwiindawo zokubeka iDekhi
Isikrini esibanjwayo
Wi-Fi Ethernet USB
Ikhamera yerobhothi igalelo lamandla
Ubonelelo lweemayile voltage Fluctuation Ubonelelo olungundoqo uguquguquko Inkqubo yokusasaza Ubonelelo lwesekethe emfutshane yangoku Ukwakhiwa kwesakhelo Iimfuno zomoya.

87 × 69 × 84 cm / 34.25 × 27 × 33 kwi (W, D, H)
88.5 kg / 195 lb 12 ANSI/SLAS-ehambelanayo kwiindawo zokubeka kwindawo yokusebenza
(ifikeleleke kwiipipettes) 4 iindawo zokubeka ezongezelelweyo ze-stagiingcebiso kunye nelebhu
(ifikeleleka kuphela kwigripper) 7-intshi ye-LCD yesikrini sokuchukumisa esinomkrwelo- kunye neGlasi yeGorilla enganyangekiyo kumonakalo 3
802.11 ac/a/b/g/n dual-band (2.4/5 GHz)
100 Mbps 9 USB-A izibuko 1 USB-B port USB 2.0 isantya
2MP, ifoto kunye nevidiyo 100 VAC, 240 Hz, 50 60 A/1 VAC, 4.0 A/115 VAC
±10%
±5%
TN-S
6.3 A
Intsimbi eqinile kunye noyilo lwe-aluminium ye-CNC
Iifestile zecala le-polycarbonate ezisuswayo kunye nomnyango wangaphambili Ubuncinci yi-20 cm / 8 phakathi kweyunithi kunye nodonga

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ISAHLUKO SE-3: INKCAZELO YENKQUBO

Iimfuno zePC ezidityanisiweyo

I-Opentrons App isebenza kwi: Windows 10 okanye kamva macOS 10.10 okanye kamva Ubuntu 12.04 okanye kamva

Iinkcukacha zokusingqongileyo

Iimeko zokusingqongileyo Iqondo lobushushu elihambelanayo Ukufuma okunxulumene nongcoliseko

Ukusetyenziswa kwangaphakathi kuphela +20 ukuya +25 °C (kunconyiwe) 40%, ukungaxinanisi (kuyacetyiswa) 60 (ungcoliseko olungaqhubekiyo kuphela)

Ngolwazi olongezelelweyo malunga neemeko ezamkelekileyo zokusingqongileyo zokusetyenziswa kunye nezothutho, jonga icandelo leMemo yokusiNgqongileyo kwisahluko soFakelo nokuFudusa.

Iziqinisekiso

Iziqinisekiso zigqityiwe Ayiqinisekiswanga/iqinisekisiweyo

CE, ETL, FCC, ISO 9001 IVD, GMP

Isishwankathelo solwazi lwesiqinisekiso siprintwa kwisincamathelisi ngasemva kweFlex, kufutshane nesitshixo sokuvula/sokucima. Ukufumana isiqinisekiso esineenkcukacha kunye nolwazi lokuthotyelwa, jonga icandelo lokuThotyelwa kweMimiselo kwiNtshayelelo.

Inombolo yesiriyeli
I-Flex nganye inenombolo eyodwa yesiriyali. Ubume benombolo yolandelelwano lunika ulwazi olongezelelweyo, kubandakanywa nomhla wokuveliswa kwerobhothi. UmzekeloampLe, inombolo yothotho FLXA1020231007001 ingabonisa:

I-OPENTRONS FLEX

ISAHLUKO SE-3: INKCAZELO YENKQUBO

Abalinganiswa FLX A10 2023 10 07 001

IYunithi yoSuku lweNyanga yodidi lweModeli yoNyaka

Intsingiselo Irobhothi yiOpentrons Flex. Ikhowudi yenguqulo yemveliso yerobhothi. Irobhothi yenziwe ngo-2023. Irobhothi yenziwe ngo-Oktobha. Irobhothi yenziwe ngomhla we-7 wenyanga. Inani elikhethekileyo leerobhothi ezenziwe ngosuku oluthile.

Ungafumana inombolo yesiriyeli yeFlex yakho:
Kwisincamathelisi sesiqinisekiso ngasemva kweFlex, kufutshane nesitshixo sokuvula/sokucima. Kwicala elingasemva lesikrini sokuchukumisa (ukuya kwindawo yokusebenza). Kwi-Opentrons App phantsi kweZixhobo> iFlex yakho> Useto lweRobhothi> Ethuthukile.

I-OPENTRONS FLEX

ISAHLUKO 4
Iimodyuli
I-Opentrons Flex idibanisa nenani leemodyuli ze-Opentrons hardware. Zonke iimodyuli zi peripherals ezihlala kwideck slots, kwaye uninzi lulawulwa yirobhothi ngaphezulu koqhagamshelo lwe-USB.
Esi sahluko sichaza imisebenzi kunye neenkcukacha eziphathekayo zeemodyuli ezihambelana nenkqubo ye-Opentrons Flex, kunye nendlela yokudibanisa nokuzilinganisa. Ukufumana iinkcukacha ezithe vetshe ngokuseta imodyuli nokusetyenziswa kwayo, jonga iimanyuwali zeemodyuli zomntu ngamnye. Ngeenkcukacha zokudibanisa iimodyuli kwiiprothokholi zakho, bona icandelo loMyili weProtocol kwisahluko soPhuhliso lweProtocol okanye amaxwebhu ePython Protocol API ekwi-intanethi.
4.1 Iimodyuli ezixhaswayo
I-Opentrons Flex iyahambelana neentlobo ezine zeemodyuli ze-Opentrons ezikwidekhi:
Imodyuli ye-Heater-Shaker ibonelela ngokufudumeza kumgangatho kunye nokungcangcazela kwe-orbital. Imodyuli inokufudunyezwa ukuya kuma-95 °C, kwaye inokugubha sampngaphantsi ukusuka 200 ukuba 3000 rpm.
Ibhloko yeMagnetic sisixhobo sokwenziwa esibambe ilebhu kufutshane nemagnethi yayo ephezulu ye-neodymium. Imodyuli ye-OT-2 yeMagnetic GEN1 kunye ne-GEN2, ezihambisa iimagnethi zazo phezulu naphantsi xa zithelekiswa nelebhu, azixhaswa kwi-Opentrons Flex.
IModyuli yeTemperature yimodyuli yepleyiti eshushu nebandayo ekwaziyo ukugcina ubushushu bemo obuzinzileyo phakathi kwe-4 kunye ne-95 °C.
Imodyuli ye-Thermocycler ibonelela nge-deck, i-thermocycling ezenzekelayo ngokupheleleyo, eyenza ukuba i-automation yamanyathelo anyukayo kunye nasezantsi. I-Thermocycler GEN2 ihambelana ngokupheleleyo ne-gripper. I-Thermocycler GEN1 ayinakusetyenziswa kunye ne-gripper, kwaye ke ayixhaswanga kwi-Opentrons Flex.
Ezinye iimodyuli eziyilelwe ekuqaleni i-OT-2 ziyahambelana neFlex, njengoko kushwankathelwe kwitheyibhile engezantsi. Uphawu lokukhangela lubonisa ukuhambelana, kwaye u-X ubonisa ukungahambelani.

I-OPENTRONS FLEX

ISAHLUKO 4: IIMODYULI

Uhlobo lwesixhobo kunye neModyuli yesizukulwana seHeater-Shaker Imodyuli yeGEN1 iModyuli yeMagnetic GEN1 Imodyuli yeMagnetic GEN2 Ibhloko yeMagnetic GEN1 Imodyuli yoBubushushu yeGEN1 Imodyuli yoBubushushu yeGEN2 Imodyuli yeThermocycler yeGEN1 Imodyuli yeThermocycler GEN2 HEPA Imodyuli

OT-2

Flex

4.2 Inkqubo yemodyuli yecaddy
Iimodyuli ezihambelanayo ziyilelwe ukuba zilingane kwiikhadi ezithatha indawo engaphantsi kwedekhi. Le nkqubo ivumela i-labware ngaphezulu kweemodyuli ukuba ihlale ikufutshane kumphezulu wedekhi, kwaye ikwavumela ukuhanjiswa kwentambo engaphantsi kwedekhi ukuze umgangatho uhlale ucocekile ngexesha leprotocol yakho ibaleka.

IiCaddies zeHeater-Shaker, iTemperature, kunye neeModyuli zeThermocycler.

I-OPENTRONS FLEX

ISAHLUKO 4: IIMODYULI
Ukufaka imodyuli kumphezulu wedekhi, kufuneka kuqala ibekwe kwimodyuli ehambelanayo yecaddy. Uhlobo ngalunye lwemodyuli ehambelanayo inoyilo lwayo lwecaddy olulungelelanisa imodyuli kunye nelebhu ngokuchanekileyo kunye nedekhi ejikelezileyo. (Imeko engafaniyo yiBlokhi yeMagnetic, engadingi amandla okanye intambo ye-USB kwaye ihlale ngokuthe ngqo kwindawo yedekhi.) I-Caddies yeemodyuli ezithatha i-slot enye zinokubekwa naphi na kwikholamu 1 okanye yesi-3; i-Thermocycler inokufakwa kuphela kwiindawo zokubeka i-A1 kunye ne-B1 ngaxeshanye.
Ngokubanzi, ukufaka imodyuli yecaddy:
1. Susa naziphi na iindawo zokubeka idekhi kwindawo apho imodyuli iya kuya khona. 2. Beka imodyuli kwikhadi yayo kwaye uqinise iiankile zayo. 3. Ukuhambisa amandla emodyuli kunye neentambo ze-USB ngaphaya kwezigqubuthelo zecala, phezulu nge-slot yedekhi engenanto, kunye
zincamathele kwimodyuli. 4. Beka imodyuli yecaddy kwindawo yokubeka kwaye uyijije endaweni yayo.
Ngemiyalelo yofako oluchanekileyo, jonga iSikhokelo sokuQala ngokukhawuleza okanye Incwadi yeMiyalelo yemodyuli ethile. Uqhagamshelo lwekhebula kunye nendlela yokuncamathela kwicadi iyahluka ngokwemodyuli.
4.3 Ulungelelwaniso lweemodyuli
Xa uqala ukufaka imodyuli kwiFlex, kufuneka usebenzise ulungelelwaniso lwendawo oluzenzekelayo. Le nkqubo ifana nokulinganisa indawo yezixhobo, kwaye iqinisekisa ukuba i-Flex ihambela kwiindawo ezichanekileyo ezichanekileyo zokusebenza kweprotocol. Ngexesha lokulinganisa, i-Flex iya kufudukela kwiindawo kwi-adapter yokulinganisa imodyuli, ebonakala ifana nezikwere zokulinganisa eziyinxalenye yeendawo zokubeka i-deck esuswayo.

Iiadaptha zokuCalibration zeHeater-Shaker, iTemperature, kunye neThermocycler Modules.
Ulungelelwaniso lwemodyuli luyafuneka kuzo zonke iimodyuli ezifakelwa ngecaddy: iHeater-Shaker, iTemperature, kunye neThermocycler Modules. Ibhloko yeMagnetic ayifuni kulungelelaniswa, kwaye ilungele ukusetyenziswa nje ukuba uyibeke kumgangatho.

I-OPENTRONS FLEX

ISAHLUKO 4: IIMODYULI
Nini ukulinganisa iimodyuli
I-Flex ikukhuthaza ngokuzenzekelayo ukuba wenze ulungelelwaniso xa uqhagamshela kwaye unika amandla kwimodyuli engenayo idatha egciniweyo yokulinganisa. (Ungasikhupha esi saziso, kodwa awuzukwazi ukuqhuba iprothokholi ngemodyuli de ube uyilinganisele.)
Wakuba ulugqibile ulungelelwaniso, iFlex igcina idatha yolungelelwaniso kunye nenombolo yesiriyali yemodyuli ukuze isetyenziswe kwixesha elizayo. I-Flex ayiyi kukukhuthaza ukuba uhlengahlengise ngaphandle kokuba ucima idatha yokulinganisa yaloo modyuli kwiisetingi zerobhothi. Unganika amandla imodyuli yakho ngokukhululekileyo kwaye uyicime, okanye uyisuse uye kwenye indawo yokubeka, ngaphandle kokufuna ukuhlaziya kwakhona. Ukuba ufuna ukuhlengahlengisa, ungaqala inkqubo nangaliphi na ixesha kwikhadi lemodyuli kwi-Opentrons App. (Uhlengahlengiso alufumaneki kwiscreen esichukumisayo.)
Ulinganisa njani iimodyuli
Imiyalelo ekwisikrini sokuchukumisa okanye kwi-Opentrons App iya kukukhokela kwinkqubo yokulinganisa. Ngokubanzi amanyathelo ngala:
1. Hlanganisa izixhobo ezifunekayo, kubandakanywa i-adapter yokulinganisa imodyuli kunye ne-pipette calibration probe. 2. Beka i-adaptha yokulinganisa kumphezulu wemodyuli kwaye uqinisekise ukuba ikwinqanaba elipheleleyo.
Ezinye iimodyuli zinokufuna ukuba uqhoboshele iadaptha kwimodyuli. 3. Qhoboshela iprobe yokulinganisa kwipayipi. 4. I-Flex iya kuhamba ngokuzenzekelayo ukuze ichukumise amanqaku athile kwi-adaptha yokulinganisa kwaye igcine ezi zinto
amaxabiso olungelelwaniso okusetyenziswa kwixesha elizayo.
Nje ukuba ulungelelwaniso lugqityiwe kwaye uyisusile iadaptha kunye nophando, imodyuli iya kuba ilungele ukusetyenziswa kwiiprothokholi.
Ngaliphi na ixesha, unako view kwaye ulawule idatha yolungelelwaniso lwemodyuli yakho kwi-Opentrons App. Yiya kwiisetingi zeRobhothi kwiFlex yakho kwaye ucofe ithebhu yoCalibration.

I-OPENTRONS FLEX

ISAHLUKO 4: IIMODYULI
4.4 Imodyuli ye-Heater-Shaker GEN1

Iimpawu zeHeater-Shaker
UKUSHISA NOKUDIKISEKA
I-Heater-Shaker ibonelela ngokufudumeza kumgangatho kunye nokungcangcazela kwe-orbital. Imodyuli inokufudunyezwa ukuya kwi-95 °C, kunye nolu bushushu bulandelayofile:
Uluhlu lobushushu: 37 °C Ukuchaneka kobushushu: ±95 °C kwi-0.5 °C Ubushushu obufanayo: ±55 °C kwi-0.5 °CRamp izinga: 10 °C/min
Imodyuli inokugubha samples ukusuka 200 ukuba 3000 rpm, kunye pro kungcangcazela zilandelayofile:
Idayamitha ye-Orbital: 2.0 mm Umkhombandlela we-Orbital: Uluhlu lwesantya se-Clockwise: 200 rpm Ukuchaneka kwesantya: ±3000 rpm
Imodyuli inelatshi yelebhu enamandla yokukhusela iipleyiti kwimodyuli phambi kokungcangcazela.

I-OPENTRONS FLEX

ISAHLUKO 4: IIMODYULI
IIADAPHATHA ZETHEMALI Iadaptha yobushushu ehambelanayo iyafuneka ukongeza ilebhu kwi-Heater-Shaker. Iiadaptha zingathengwa ngokuthe ngqo kwi-Opentrons apha https://shop.opentrons.com. Iiadaptha zeThermal ezikhoyo ngoku zibandakanya:

Iadaptha yeFlethi yeHlabathi

Iadaptha yePCR

I-Adapter ye-Deep Well

96 Isiadaptha esiMphantsi esisicaba

ULAWULO LWESOFTWARE
I-Heater-Shaker icwangciswe ngokupheleleyo kwiProtocol Designer kunye nePython Protocol API. I-API yePython ivumela ukuba amanye amanyathelo eprotocol aqhutywe ngokufanayo ngelixa i-Heater-Shaker isebenza. Jonga imiyalelo engathinteliyo kuxwebhu lwe-API ngeenkcukacha ekongezeni amanyathelo ahambelanayo kwiiprothokholi zakho.
Ngaphandle kweeprothokholi, i-Opentrons App ingabonisa imeko yangoku ye-Heater-Shaker kwaye inokulawula ngokuthe ngqo isifudumezi, i-shaker, kunye nelatshi yelebhu.

I-OPENTRONS FLEX

ISAHLUKO 4: IIMODYULI

Iinkcazo zesifudumezi-Shaker

Imilinganiselo Ubunzima Igalelo lemodyuli yamandla Igalelo kwiadaptha yamandla Ubonelelo olungundoqo voltage Fluctuation Overvoltage Ukusetyenziswa kwamandla

152 × 90 × 82 mm (L/W/H) 1.34 kg 36 VDC, 6.1 A 100 VAC, 240/50 Hz ±60% Udidi II Ukungenzi nto: 10 W

Umzekelo: Ukungcangcazela: 4 W Ukufudumeza: 11 W Ukufudumeza nokungcangcazela: 10 W

Iimeko zokusingqongileyo Iqondo lobushushu elihambelanayo Umphakamo wongcoliseko iqondo

Ubuninzi: 125 W Ukusetyenziswa kwangaphakathi kuphela ngama-130 °C Ukuya kuma-20%, ukungaxinanisi Ukuya kuthi ga kwi-25 m ngaphezu komphakamo wolwandle 80

I-OPENTRONS FLEX

ISAHLUKO 4: IIMODYULI
4.5 Ibhloko yeMagnetic GEN1

Iimpawu zeBlock Magnetic
I-Opentrons Magnetic Block GEN1 yimagnethi yesibambi sepleyiti esinama-96. Iibhloko zemagneti zisetyenziswa kwiiprothokholi ezixhomekeke kwimagnetism ukutsala amasuntswana ngaphandle kokunqunyanyiswa kwaye uwagcine kwiipleyiti zamaqula ngexesha lokuhlamba, lokuhlanjululwa, okanye ezinye iinkqubo zokuhlambulula. Umzekeloample, ulungiselelo lweNGS oluzenzekelayo; ukucoca i-DNA ye-genomic kunye ne-mitochondrial, i-RNA, okanye iiprotheni; kunye nezinye iinkqubo zokutsalwa kuzo zonke iimeko zokusetyenziswa ezinokubandakanya iibhloko zemagneti.
IZIQULATHO ZAMAZIBANE
Ibhloko yeMagnetic ayinawo amandla, ayiqulathanga naziphi na izinto ze-elektroniki, kwaye ayishukumisi amaso kazibuthe phezulu okanye ezantsi kwisisombululo. Amaqula aquka i-96 high-high-high-forth neodymium ring magnets egxininiswe kwibhedi elayishwe entwasahlobo, enceda ukugcina ukunyamezela phakathi kwebhloko kunye neepipettes ngelixa kuqhuba iiprothokholi ezizenzekelayo.
ULAWULO LWESOFTWARE
I-Magnetic Block GEN1 icwangciswe ngokupheleleyo kwiProtocol Designer kunye nePython Protocol API.

I-OPENTRONS FLEX

ISAHLUKO 4: IIMODYULI

Ngaphandle kweeprothokholi, nangona kunjalo, isikrini esichukumisayo kunye ne-Opentrons App ayazi kwaye ayikwazi ukubonisa isimo sangoku seMagnetic Block GEN1. Le yimodyuli engenamandla. Ayinayo i-electronic or mechanical components enokunxibelelana ne-Flex robot. “Ulawula” iBhloko yeMagnetic usebenzisa iprothokholi ezisebenzisa i-Opentrons Flex Gripper ukongeza nokususa ilebhu kule modyuli.

Iimpawu zeBlock yeMagnetic

Imilinganiselo Ubunzima Imodyuli amandla Ibakala lemagnethi Iimeko zokusingqongileyo Iqondo lobushushu eliphakathi Ukufuma okuhambelanayo Umphakamo woNgcoliseko isidanga

136 × 94 × 45 mm (L/W/H) 1.13 kg Akukho, imodyuli ayinamandla N52 neodymium Ukusetyenziswa kwangaphakathi kuphela 20 °C 25%, non-condensing Ukuya kwi-30 m ngaphezu komgangatho wolwandle 80

I-OPENTRONS FLEX

ISAHLUKO 4: IIMODYULI
4.6 Imodyuli yobushushu GEN2

Iimpawu zeModyuli yobushushu
UKUSHISA NOKUPHOLA
I-Opentrons Temperature Module GEN2 yimodyuli yepleyiti eshushu nebandayo. Ihlala isetyenziswa kwiiprothokholi ezifuna ukufudumeza, ukupholisa, okanye utshintsho lobushushu. Imodyuli inokufikelela kwaye igcine amaqondo obushushu ukusuka kwi-4 °C ukuya kwi-95 °C ngaphakathi kwemizuzu, kuxhomekeke kuqwalaselo lwemodyuli kunye nemixholo.
Iibhloko ezishushu
Ukubamba i-labware kwiqondo lokushisa, imodyuli isebenzisa iibhloko ze-aluminium thermal. Imodyuli iza ne-24well kunye neebhloko ze-thermal ezingama-96. I-Temperature Module caddy iza nebhloko enzulu yequla kunye nebhloko esezantsi ethe tyaba eyenzelwe ukusetyenziswa kunye neFlex Gripper. Iibhloko zibamba i-1.5 mL kunye ne-2.0 mL iityhubhu, iipleyiti ze-PCR ezingama-96, imichilo ye-PCR, iipleyiti ezinzulu, kunye neepleyiti ezisezantsi.

I-OPENTRONS FLEX

ISAHLUKO 4: IIMODYULI
Qaphela: Imodyuli ikwahamba ngenqanawa enebhloko esezantsi ethe tyaba yeOT-2. Musa ukusebenzisa ibhloko ye-OT-2 ngeFlex. Ibhloko esezantsi emcaba yeFlex inamagama athi “Opentrons Flex” kumphezulu wayo. Le ye-OT-2 ayifuni.

Ibhloko ye-thermal engama-24

Ibhloko ye-thermal engama-96

Ibhloko ye-thermal enzulu

Ibloko ye-thermal ephantsi yeFlex

AMANZI OKUHLAMBA NOKUFUDUMISA
Ngenxa yokuba umoya uyinto yokukhusela i-thermal, izithuba phakathi kwe-labware kunye nebhloko ye-thermal inokuchaphazela ukusebenza kwexesha lobushushu beModyuli yoBubushushu. Ukubeka amanzi amancinci kwiibhloko ze-thermal ze-24 okanye ze-96 zisusa izithuba zomoya kunye nokuphucula ukusebenza kakuhle kokufudumeza. Ubungakanani obufanelekileyo bamanzi buxhomekeke kwibhloko ye-thermal kunye ne-labware. Jonga iPhepha leNgcaciso yeModyuli yoBuqondo ukuze ufumane iingcebiso ezingaphezulu.

ULAWULO LWESOFTWARE
Imodyuli yobushushu icwangciswe ngokupheleleyo kwiProtocol Designer kunye nePython Protocol API.
Ngaphandle kweeprothokholi, i-Opentrons App ingabonisa imeko yangoku yeModyuli yoBubushushu kwaye inokulawula ngokuthe ngqo ubushushu bepleyiti yomphezulu.

I-OPENTRONS FLEX

ISAHLUKO 4: IIMODYULI

Imilinganiselo yeModyuli yobushushu

Imilinganiselo yobunzima beModyuli amandla
Iimeko zokusingqongileyo Iqondo lobushushu elihambelanayo Umphakamo wongcoliseko iqondo

194 × 90 × 84 mm (L/W/H) 1.5 kg Igalelo: 100 VAC, 240/50 Hz, 60 A Imveliso: 4.0 VDC, 36 A, 6.1 W max Ukusetyenziswa kwangaphakathi kuphela <219.6 °C (eyona nto inconywa ukuba iphole kakuhle ) Ukuya kuthi ga kwi-22%, engaxinanisi Ukuya kuthi ga kwi-60 m ngaphezu komphakamo we-2000 wolwandle

4.7 Imodyuli yeThermocycler GEN2

I-OPENTRONS FLEX

ISAHLUKO 4: IIMODYULI
Iimpawu zeThermocycler
I-Opentrons Thermocycler Module GEN2 yi-thermocycler esebenza ngokuzenzekelayo kwidekhi, ibonelela nge-PCR engenazandla kwifomathi yepleyiti engama-96. Isivalo sayo esishushu kunye nesitywina esilahlwayo silingana ngokuqinileyo phezu kwepleyiti, iqinisekisa i-s esebenzayoample ukufudumeza kunye nokunyuka komphunga okuncinci.
UKUSHISA NOKUPHOLISA Ibhloko yeThermocycler inokufudumeza kwaye ipholile, kwaye isiciko sayo sinokufudumeza, kunye nolu bushushu bulandelayo.file: Uluhlu lweqondo lobushushu block Thermal: 4 °C Thermal block ubuninzi ukufudumeza ramp izinga: 4.25 °C/s ukusuka kwi-GEN2 ukuya kuma-95 °C ibhloko ye-Thermal yokupholisa ubuninzi ramp izinga: 2.0 °C / s ukusuka kwi-95 °C ukuya kwi-ambient Uluhlu lobushushu be-Lid: 37 °C Ukuchaneka kobushushu be-Lid: ± 110 °C Isivalo esizenzekelayo sinokuvulwa okanye sivalwe njengoko kufuneka ngexesha lokwenziwa kweprotocol.
THERMOCYCLER PROFILES IThermocycler inokuphumeza iprofiles: ukukhwela ibhayisekile ngokuzenzekelayo ngokulandelelana kwamaqondo obushushu ebhloko ukwenza ubushushu obunobuzaza.
I-RUBBER AUTOMATION SEALS I-Thermocycler iza kunye ne-rubber automation seals ukunceda ukunciphisa ukuvela kwe-evaporation. Isitywina ngasinye kufuneka sicocwe phambi kokusetyenziswa kwaye singasetyenziselwa imitsi emininzi. Izitywina ezongezelelweyo zingathengwa ngokuthe ngqo kwi-Opentrons apha https://shop.opentrons.com.
ULAWULO LWESOFTWARE I-Thermocycler icwangciswe ngokupheleleyo kwiProtocol Designer kunye nePython Protocol API. Ngaphandle kweeprothokholi, i-Opentrons App inokubonisa imeko yangoku ye-Thermocycler kwaye inokulawula ngokuthe ngqo ubushushu bebhloko, ubushushu besiciko, kunye nesikhundla sesiciko.

I-OPENTRONS FLEX

ISAHLUKO 4: IIMODYULI

Iimpawu zeThermocycler

Imilinganiselo (isiciko sivulekile) Imilinganiselo (isiciko sivaliwe) Ubunzima (kuquka umbhobho ongasemva) Iadaptha yombane voltage Iadaptha yamandla yangoku Overvoltage Iimeko zokusingqongileyo Iqondo lobushushu elihambelanayo Ukufuma okuyeleleneyo Umphakamo Iimfuneko zokungena komoya

244.95 × 172 × 310.1 mm (L/W/H) 244.95 × 172 × 170.35 mm (L/W/H) 8.4 kg 100 V ku-240/50 Hz 60 A Udidi II Ukusetyenziswa kwangaphakathi 8.5 °C5 ngaphakathi kuphela 20 °C (eyamkelekileyo) 25%, engajikiyo Ukuya kuma-2 m ngaphezu komphakamo wolwandle Ubuncinane yi-40 cm / 30 phakathi kweyunithi kunye nodonga

I-OPENTRONS FLEX

ISAHLUKO 5
Ilebhu
Esi sahluko sigubungela izinto ezikwiThala leencwadi le-Opentrons onokulisebenzisa ne-Opentrons Flex kunye ne-Opentrons Flex Gripper. Ikwagquma ilebhu yesiko kwaye, kubasebenzisi bethu bamandla, inxibelelanisa izinto zelebhu kwi-JSON yazo ehambelanayo. file iinkcazelo.
Ungathenga ilebhu kubenzi bezixhobo zokuqala okanye kwivenkile yeOpentrons apha https://shop.opentrons.com. Kwaye, i-Opentrons isoloko isebenza ukuqinisekisa iinkcazelo zelebhu entsha. Jonga iLayibrari yeLayibrari (edityaniswe ngasentla) kuluhlu lwamva nje.
5.1 Iikhonsepthi zeLabware
ILabware ibandakanya ngaphezu kwezinto ezibekwe kumgangatho kwaye zisetyenziswe kwiprotocol. Ukukunceda uqonde i-Opentrons labware, masijonge esi sihloko kwiimbono ezintathu ezahlukeneyo. Kwi-Opentrons Flex, i-labware ibandakanya izinto kwiLayibrari yeLayibrari yethu, idatha echaza iqhekeza ngalinye lelebhu, kunye nelebhu yesiko.
Ilebhu njengehardware
ILayibrari yeLabhu ibandakanya yonke into onokuthi uyisebenzise ngokuzenzekelayo nge-Opentrons Flex. La ngamalungu ahlala ixesha elide kunye nezinto ezisebenzisekayo osebenza ngazo, uphinda usebenzise, okanye uzilahle ngelixa uqhuba iprotocol. Awudingi ukuthatha nawaphi na amanyathelo awodwa ukuze usebenze ngezinto ezikwiThala leencwadi leLabware. Irobhothi yeFlex iyayazi indlela yokusebenza nayo yonke into ekwithala leencwadi ngokuzenzekelayo.
Ilebhu njengedatha
Ulwazi lweLebhu lugcinwe kwi Javascript object notation (JSON) files kunye .json file izandiso. A JSON file ibandakanya imilinganiselo yendawo (ubude, ububanzi, ukuphakama), umthamo wevolumu (L, mL), kunye nezinye iimetriki ezichaza iimpawu zomphezulu, iimilo zazo kunye neendawo. Xa usebenzisa iprotocol, iFlex ifunda ezi .json files ukwazi ukuba yeyiphi i-labware ekwidekhi kunye nendlela yokusebenza ngayo.

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE
Ilebhu yesiko
Ilebhu yesiko yilebhu engaqukwanga kwiLayibrari yeLabware okanye ilebhu eyenziwe nguMyili weLabware yesiNtu. Nangona kunjalo, ngamanye amaxesha umbono welebhu yesiko ufika unomthwalo weengcinga ezinzima, indleko, okanye ubunzima. Kodwa, ilebhu yesiko akufuneki ibenzima ukuyiqonda okanye ukuyila. Masithathe umzuzwana sityhile ingqikelelo yelebhu yesiko.
Njenge example, Opentrons Labware Library ibandakanya 96-iqula iipleyiti (200 L) ukusuka Corning kunye BioRad, kodwa abanye abavelisi benza ezi pleyiti kakuhle kakhulu. Kwaye, ngenxa yemigangatho yoshishino eqhelekileyo, iyantlukwano phakathi kwezi zinto zikhoyo kwilebhu zincinci. Nangona kunjalo, i-200 L eqhelekileyo, ipleyiti yequla engama-96 esuka kwiStellar Scientific, Oxford Lab, okanye iKrackeler Scientific (okanye nawuphi na omnye umboneleli ngalo mba) “yinkqubo yesiko” yeFlex kuba ayichazwanga kwangaphambili kwiLayibrari yethu yeLabware. . Ukongeza, umahluko omncinci kwimilinganiselo yelebhu unokuba nempembelelo enkulu kwimpumelelo yeprotocol yakho. Ngesi sizathu, kubalulekile ukuba nengcaciso echanekileyo yelebhu yelebhu nganye ofuna ukuyisebenzisa kwiprothokholi yakho.
Kwakhona, ngelixa i-labware yesiko inokuba yi-esoteric, iqhekeza elinye lekhithi, ixesha elininzi ziingcebiso, iipleyiti, iityhubhu, kunye neerack ezisetyenziswa yonke imihla kwiilebhu kwihlabathi liphela. Kwakhona, umahluko phakathi kwe-Opentrons ilebhu kunye nelebhu yesiko yinto entsha ayichazwanga kwangaphambili kwisoftware enika irobhothi amandla. I-Flex inako, kwaye yenza, isebenze nezinye izinto ezisisiseko zelebhu okanye into eyodwa, kodwa kufuneka urekhode iimpawu zaloo nto kwinkcazelo yelebhu ye-JSON. file kwaye ungenise loo datha kwi-Opentrons App. Bona icandelo leeNgcaciso zeLabhure yesiNtu engezantsi ukuze ufumane ulwazi oluthe kratya.
Ukushwankathela, i-labware ibandakanya:
Yonke into ekwithala leencwadi le-Opentrons. Iinkcazelo zeLabhure: idatha kwi-JSON file echaza iimilo, ubungakanani, kunye nesakhono sezinto ezizimeleyo
njengeepleyiti zamaqula, iingcebiso, iireservoirs, njl. njl. Isiko elebhu, ezizizinto ezingaqukwanga kwiLayibrari yeLabhu.
Emva kwe-reviewXa siqwalasela ezi ngqikelelo zibalulekileyo, makhe sihlolisise iindidi kunye nezinto kwiThala leencwadi leLayibrari ye-Opentrons. Emva koko, siya kugqibezela isahluko ngokuphelaview yamacandelo edatha yelebhu file kwaye ushwankathele iimpawu zeOpentrons kunye neenkonzo ezikunceda wenze ilebhu yesiko.

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE

5.2 Amadama
I-Opentrons Flex isebenza ngokungagqibekanga kunye namadama amaqula amaninzi adweliswe ngezantsi. Ukusebenzisa la machibi kunceda ukunciphisa umthwalo wakho womsebenzi wamalungiselelo ngenxa yokuba zilungele ukuzenzela kanye ngaphandle kwebhokisi. Ulwazi ngedama lukwafumaneka kwiThala leeNcwadi le-Opentrons Labware.

Amadama anequla elinye

Iinkcukacha zomenzi

Agilent

290 mL V ezantsi

I-axygen

90 mL Umphantsi oMcaba

I-NEST

195 mL Umphantsi oMcaba

290 mL V ezantsi

Igama lokulayisha i-API
agilient_1_ reserve_290ml
i-axygen_1_ indawo yokugcina_90ml
nest_1_ reservoir_195ml
nest_1_ reservoir_290ml

Amachibi amaqula amaninzi

Iinkcukacha zomenzi

I-NEST

12 imithombo 15 mL / equleni V ezantsi

EzobuNzululwazi zase-USA

12 imithombo 22 mL / equleni V ezantsi

Igama lomthwalo we-API nest_12_ reservoir_15ml
usascientific_12_ reserve_22ml

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE
Amadama kunye neenkcazo ze-API
Ithala leencwadi le-Opentrons Labware lichaza iimpawu zeendawo zokugcina amanzi ezidweliswe ngasentla kwi-JSON eyahlukileyo files. Irobhothi kunye ne-Opentrons Python API zixhomekeke kwezi nkcazelo ze-JSON ukuze zisebenze ngeelebhu esetyenziswa yimigaqo yakho. Umzekeloample, xa usebenza nge API, i ProtocolContext.load_labware umsebenzi wamkela la magama alebhu njenge parameters ezisebenzayo kwikhowudi yakho. Amagama omthwalo we-API adityanisiweyo aqhagamshela kwiinkcazelo zelebhu yereservoir kwindawo yokugcina ye-Opentrons GitHub.
Ilebhu yesiko logcino
Zama ukuyila inkcazelo yelebhu yesiko kunye noMdali weLabware ye-Opentrons ukuba uvimba ongathanda ukuwusebenzisa awudweliswanga apha. Inkcazo yesiko idibanisa yonke imilinganiselo, idata, imilo, umthamo wevolumu, kunye nolunye ulwazi kwi-JSON. file. I-Opentrons Flex idinga olu lwazi ukuze iqonde indlela yokusebenza ngelebhu yesiko lakho. Bona icandelo leeNgcaciso zeLabhu yesiSiko ngolwazi oluthe kratya.
5.3 Iipleyiti zamaqula
I-Opentrons Flex isebenza ngokungagqibekanga kunye neepleyiti zamaqula ezidweliswe ngezantsi. Ukusebenzisa ezi pleyiti zamanzi kunceda ukunciphisa umthwalo wakho womsebenzi wamalungiselelo kuba zilungele ukuzenzela kanye ngaphandle kwebhokisi. Ulwazi ngepleyiti yeWell lukwafumaneka kwiThala leeNcwadi le-Opentrons Labware.

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE

Iipleyiti ezi-6
Umenzi weCorning

Iinkcukacha
Amaqula ama-6 16.8 mL/equleni Amaqula asetyhula, umzantsi osicaba

Igama lomthwalo we-API corning_6_wellplate_16.8ml_flat

Iipleyiti ezi-12
Umenzi weCorning

Iinkcukacha
Amaqula ama-12 6.9 mL/equleni Amaqula asetyhula, umzantsi osicaba

Igama lomthwalo we-API corning_12_wellplate_6.9ml_flat

Iipleyiti ezi-24
Umenzi weCorning

Iinkcukacha
Amaqula ama-24 3.4 mL/equleni Amaqula asetyhula, umzantsi osicaba

Igama lomthwalo we-API corning_24_wellplate_3.4ml_flat

Iipleyiti ezi-48
Umenzi weCorning

Iinkcukacha
Amaqula ama-48 1.6 mL/equleni Amaqula asetyhula, umzantsi osicaba

Igama lomthwalo we-API corning_48_wellplate_1.6ml_flat

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE

Iipleyiti ezi-96
Umenzi we-Bio-Rad Corning NEST
Opentrons Thermo Scientific
EzobuNzululwazi zase-USA

Iinkcukacha
Amaqula angama-96 200 µL/iqula Amaqula asetyhula, V emazantsi
Amaqula angama-96 360 µL/iqula Amaqula asetyhula, umzantsi osicaba
Amaqula angama-96 100 µL/equleni Amaqula asesetyhula, V ezantsi PCR isiketi esigcweleyo
Amaqula angama-96 200 µL/iqula Amaqula asetyhula, umzantsi osicaba
Amaqula anzulu angama-96 2000 µL/amaqula asisikwere, V emazantsi
Amaqula angama-96 amaqula angama-200 µL/amaqula aSetyhula, V ezantsi PCR isiketi esigcweleyo
I-Nunc 96 yemithombo enzulu 1300 µL/iqula eliSetyhula, U ezantsi
I-Nunc 96 yemithombo enzulu 2000 µL/iqula eliSetyhula, U ezantsi
Amaqula anzulu angama-96 2.4 mL/amaqula asisikwere, u-U ezantsi

Igama lokulayisha i-API biorad_96_wellplate_200ul_pcr
corning_96_wellplate_360ul_flat
nest_96_wellplate_100ul_pcr_full_skirt
nest_96_wellplate_200ul_flat
nest_96_wellplate_2ml_deep
opentrons_96_wellplate_200ul_pcr_full_ skirt
thermoscientificnunc_96_wellplate_ 1300ul thermoscientificnunc_96_wellplate_ 2000ul usascientific_96_wellplate_2.4ml_deep

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE

Iipleyiti ezi-384

Umenzi oSetyenziselwe iBiosystems yeBio-Rad
Corning

Iinkcukacha
Amaqula angama-384 40 µL/iqula Amaqula asetyhula, V emazantsi
Amaqula angama-384 50 µL/iqula Amaqula asetyhula, V emazantsi
Amaqula angama-384 112 µL/iqula Amaqula asisikwere, umzantsi osicaba

Igama lomthwalo we-API appliedbiosystemsmicroamp_384_ wellplate_40ul biorad_384_wellplate_50ul
corning_384_wellplate_112ul_flat

Iiadaptha zeplate kakuhle
Iipleyiti ze-aluminiyam ezidweliswe ngezantsi ziiadaptha ze-thermal zemodyuli ye-Opentrons Heater-Shaker GEN1. Ungasebenzisa ezi nkcazelo zeadaptha ezizimeleyo ukulayisha i-Opentrons eqinisekisiweyo okanye ilebhu yesiko phezu kwe-Heater-Shaker.

Uhlobo lweAdapter iOpentrons Universal Flat heater-Shaker Adapter Opentrons 96 PCR Heater-Shaker Adapter Opentrons 96 Deep Well-Shaker Adapter Opentrons 96 Isifudumezi esisemazantsi esiShaker

Igama lokulayisha i-API opentrons_universal_flat_adapter opentrons_96_pcr_adapter opentrons_96_deep_well_adapter opentrons_96_flat_bottom_adapter

Ungalayisha kwakhona zombini iadaptha kunye nelebhu ngenkcazelo enye. Ithala leencwadi lethu leLayibrari libandakanya iadaptha yobushushu esele iqwalaselwe kwangaphambili kunye nemidibaniso yelebhu eyenza i-Heater-Shaker ilungele ukusebenzisa ngaphandle kwebhokisi.

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE

Qaphela: Sukusebenzisa inkcazo edityanisiweyo ukuba ufuna ukuhambisa ilebhu kwi okanye kwi-Heater-Shaker ngexesha leprotocol yakho, nokuba yigripper okanye ngesandla. Sebenzisa inkcazo yeadaptha ezimeleyo endaweni yoko.

I-adapter/ilebhu indibaniselwano

Igama lokulayisha i-API

I-Opentrons 96 i-Adapter ye-Heater-Shaker enzulu ene-NEST yePlati eNzulu ye-2 mL

opentrons_96_deep_well_adapter_nest_ wellplate_2ml_deep

I-Opentrons 96 iFlethi ePhantsi yeSifudumezi-Isifudumezi-Adaptha esine-NEST 96 yePlati yeSihle engama-200 µL eFlethi

opentrons_96_flat_bottom_adapter_nest_ wellplate_200ul_flat

I-Opentrons 96 PCR heater-Shaker Adapter ene-NEST Well Plate 100 µL

opentrons_96_pcr_adapter_nest_wellplate_ 100ul_pcr_full_skirt

I-Opentrons Universal Flat heater-Shaker Adapter eneCorning 384 Well Plate 112 µL Flat

opentrons_universal_flat_adapter_corning_384_ wellplate_112ul_flat

Iiadaptha zingathengwa ngokuthe ngqo kwi-Opentrons apha https://shop.opentrons.com.

Iipleyiti zeWell kunye neenkcazo ze-API
Ithala leencwadi le-Opentrons Labware lichaza iimpawu zeepleyiti zequla ezidweliswe ngasentla kwi-JSON eyahlukileyo files. Irobhothi yeFlex kunye ne-Opentrons Python API zixhomekeke kwezi nkcazo ze-JSON ukuze zisebenze nge-labware esetyenziswa yimigaqo yakho. Umzekeloample, xa usebenza nge API, i ProtocolContext.load_labware umsebenzi wamkela la magama alebhu njenge parameters ezisebenzayo kwikhowudi yakho. Amagama omthwalo we-API adityanisiweyo aqhagamshela kwiinkcazo zelebhu yepleyiti kwindawo yokugcina i-Opentrons GitHub.

Custom ipleyiti kakuhle labware
Zama ukusebenzisa i-Opentrons Labware Creator ukwenza inkcazelo yelebhu yesiko ukuba ipleyiti yequla ongathanda ukuyisebenzisa ayidweliswanga apha. Inkcazo yesiko idibanisa yonke imilinganiselo, idata, imilo, umthamo wevolumu, kunye nolunye ulwazi kwi-JSON. file. I-Opentrons Flex ifunda olu lwazi ukuqonda indlela yokusebenza ngelebhu yesiko lakho. Bona icandelo leeNgcaciso zeLabhu yesiSiko ngolwazi oluthe kratya.

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE

5.4 Iingcebiso kunye neerekhi zeengcebiso
Iingcebiso ze-Opentrons Flex ziza ngama-50 µL, 200 µL, kunye ne-1000 µL ubukhulu. Ezi zingcebiso ezicacileyo, ezingaqhubekiyo ze-polypropylene ezifumanekayo okanye ngaphandle kwezihluzo. Zipakishwe zinyumba kwiiracks ezigcina iingcebiso ezingama-96 kwaye azinayo i-DNase, i-RNase, i-protease, i-pyrogens, i-DNA yomntu, i-endotoxins, kunye ne-PCR inhibitors. IiRakhi zikwabandakanya amanani eqashiso kunye nemihla yokuphelelwa kwexesha.
Iingcebiso ze-Flex pipette zisebenza kunye ne-Opentrons Flex 50 µL kunye ne-1000 µL yeepipette ku-1-, 8-, kunye no-96-bumbeko lwetshaneli. Nangona unako ukubeka naziphi na iingcebiso ze-Flex kwi-pipettes ye-50 L kunye ne-1000 L, zama ukufanisa i-tip kumthamo we-pipette. UmzekeloampLe, inokuba yinto engaqhelekanga ukubeka i-tip ye-1000 L kwi-50 L pipette. Kwi-pipette ye-1000 L, ungasebenzisa ngokuqinisekileyo i-50 L, i-200 L, okanye i-tip ye-1000 L.

Iirack zengcebiso
Iingcebiso ezingahluzwanga kunye nezihluziweyo zidityaniswe kwi-rack equkethe i-plate yesiseko ephinda isetyenziswe, i-middle-plate ephethe iingcebiso ze-96, kunye nesivalo.

Irekhi yengcebiso ngokomthamo 50 µL 200 µL 1000 µL

Igama lokulayisha i-API
Ayihluzwanga: opentrons_flex_96_tiprack_50ul Ihluziwe: opentrons_flex_96_filtertiprack_50ul
Ayihluzwanga: opentrons_flex_96_tiprack_200ul Ihluziwe: opentrons_flex_96_filtertiprack_200ul
Ayihluzwanga: opentrons_flex_96_tiprack_1000ul Ihluziwe: opentrons_flex_96_filtertiprack_1000ul

Ukunceda ngokuchongwa, i-tip rack mid-plates inekhowudi yombala ngokusekwe kubungakanani bengcebiso:
50 µL: magenta 200 L: mthubi 1000 µL: bhlowu

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE

Xa u-odola okanye uodola ngokutsha, iingcebiso kunye neeracks ziza kwiindlela ezimbini ezahlukeneyo zokupakishwa:
IiRakhi: Ziqulathe iirakhi ezisongelwe ngokwahlukeneyo (iplate yesiseko, ipleyiti ephakathi enengcebiso, kunye nesiciko). Ubume be-Racked bungcono xa ucoceko lubalulekile, ukunqanda ukungcoliseka, okanye xa iiprothokholi zakho zingakuvumeli ukuphinda kusetyenziswe iplate yesiseko okanye inxalenye.
Ukuzaliswa kwakhona: Iquka irack enye epheleleyo (ipleyiti esisiseko, ipleyiti ephakathi enamacebiso, kunye nesiciko) kunye nezikhongozeli zencam ezizimeleyo. Ulungelelwaniso lokuzalisa kwakhona lungcono xa iiprothokholi zakho zivumela iplate yesiseko okanye ukusetyenziswa kwakhona kwelungu.
Ukuhambelana kweTippipette
Iingcebiso ze-Flex pipette zenzelwe i-Opentrons Flex pipettes. Iingcebiso ze-Flex azikho ngasemva zihambelana ne-Opentrons OT-2 pipettes, kwaye awukwazi ukusebenzisa iingcebiso ze-OT-2 kwii-Flex pipettes.
Ezinye iingcebiso zomgangatho woshishino zinokusebenza kunye neepipettes zeFlex, kodwa oku akukhuthazwa. Ukuqinisekisa ukusebenza ngokupheleleyo, kufuneka usebenzise iingcebiso ze-Opentrons Flex kuphela ngeepipettes zeFlex.
I-adaptha yokubeka ingcebiso
I-pipette ye-96-channel idinga i-adapter ukuba ifake i-rack epheleleyo yeengcebiso ngokufanelekileyo. Ngethuba lenkqubo yokuncamathisela, i-pipette ihamba phezu kwe-adapter, iyancipha ngokwayo kwizikhonkwane zokukhwela, kwaye idonsa iingcebiso kwiipayipi ngokuphakamisa i-adapter kunye ne-tip rack.

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE

Qaphela: Sebenzisa kuphela i-adaptha ye-tip rack xa uthatha i-rack epheleleyo yeengcebiso kanye. Beka i-tip racks ngqo kwidekhi xa uthatha iingcebiso ezimbalwa.
Isilumkiso: Pinch point ingozi. Gcina izandla kude ne-adapter ye-tip rack ngelixa i-pipette idibanisa iingcebiso ze-pipette.

I-adaptha yohlobo lwe-Opentrons Flex 96 I-Adapter ye-Tip Rack

Igama lokulayisha i-API opentrons_flex_96_tiprack_adapter

I-adapter ye-tip rack ihambelana ne-Opentrons Flex Gripper. Ungasebenzisa into yokubamba ukubeka iiracks ezintsha kwi-adaptha okanye ukuchola kwaye uhambise iiracks ezisetyenzisiweyo kwi-chute yenkunkuma.

5.5 Iityhubhu kunye nee-tube racks

Inkqubo ye-Opentrons 4-in-1 ye-Tube Rack isebenza kunye ne-Opentrons Flex ngokungagqibekanga. Ukusebenzisa i-4-in-1 ye-tube rack kunceda ukunciphisa umthwalo wakho wokulungiselela kwangaphambili ngenxa yokuba indibaniselwano ebonelela ngayo i-automation-ready right out of the box. Ulwazi oluthe vetshe lukwafumaneka kwiThala leeNcwadi le-Opentrons Labware.

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE
Umbhobho kunye ne-rack indibaniselwano
I-Opentrons ye-4-in-1 ye-tube rack isekela ubuninzi beetyhubhu ezahlukeneyo, zodwa okanye ngobukhulu obuhlukeneyo (ivolumu) indibaniselwano. Ezi ziquka: i-6-tube rack ye-50 mL iityhubhu (6 x 50 mL). I-10-tube yokudibanisa i-tube ye-tube ezine ze-50 mL kunye neebhubhu ezintandathu ze-15 mL (4 x 50 mL, 6 x 15 mL). I-15-tube rack ye-15 mL iityhubhu (15 x 15 mL). I-24-tube rack ye-0.5 mL, 1.5 mL, okanye i-2 mL iityhubhu (24 x 0.5 mL, 1.5 mL, 2 mL).
Qaphela: Zonke iityhubhu zi-cylindrical ezine-V-shaped (conical) emazantsi ngaphandle kokuba kuboniswe ngenye indlela.

6-ityhubhu iiracks
Uhlobo lombhobho 6 Falcon 50 mL 6 NEST 50 mL
10-ityhubhu iiracks
Uhlobo lombhobho 4 uFalcon 50 mL 6 Falcon 15 mL 4 NEST 50 mL 6 NEST 15 mL

Igama lomthwalo we-API opentrons_6_tuberack_falcon_50ml_conical opentrons_6_tuberack_nest_50ml_conical
API load name opentrons_10_tuberack_falcon_4x50ml_6x15ml_conical opentrons_10_tuberack_nest_4x50ml_6x15ml_conical

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE

15-ityhubhu iiracks
Uhlobo lombhobho 15 Falcon 15 mL 15 NEST 15 mL

Igama lomthwalo we-API opentrons_15_tuberack_falcon_15ml_conical opentrons_15_tuberack_nest_15ml_conical

24-ityhubhu iiracks

Uhlobo lweThubhu
24 Eppendorf Safe-Lock 1.5 mL 24 Eppendorf Safe-Lock 2 mL, U-shaped uzantsi 24 generic 2 mL screw cap 24 NEST 0.5 mL screw cap 24 NEST 1.5 mL screw cap 24 NEST 1.5 mL snap 24 mL snap 2 m I-NEST 24 mL i-snap cap, i-U-shape ezantsi

Igama lomthwalo we-API opentrons_24_tuberack_eppendorf_1.5ml_safelock_snapcap opentrons_24_tuberack_eppendorf_2ml_safelock_snapcap
opentrons_24_tuberack_generic_2ml_screwcap opentrons_24_tuberack_nest_0.5ml_screwcap opentrons_24_tuberack_nest_1.5ml_screwcap opentrons_24_tuberack_nest_1.5ml_snapcap opentrons_24_tuberack_nest_2ml_screwcap opentrons_24_tuberack_nest_2ml_snapcap

Iinkcazo ze-API yokubeka iTube
Ithala leencwadi le-Opentrons Labware lichaza iimpawu ze-tube racks ezidweliswe ngasentla kwi-JSON eyahlukileyo files. Irobhothi yeFlex kunye ne-Opentrons Python API zixhomekeke kwezi nkcazo ze-JSON ukuze zisebenze nge-labware esetyenziswa yimigaqo yakho. Umzekeloample, xa usebenza nge API, i ProtocolContext.load_labware umsebenzi wamkela la magama alebhu njenge parameters ezisebenzayo kwikhowudi yakho. Amagama omthwalo we-API adityanisiweyo aqhagamshela kwiinkcazo zelebhu yokubeka i-tube kwindawo yokugcina i-Opentrons GitHub.

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE
Ilebhu yerekhi yesiko
Zama ukwenza inkcazelo yelebhu yesiko usebenzisa i-Opentrons Labware Creator ukuba umbhobho kunye nerekhi indibaniselwano ongathanda ukuyisebenzisa ayidweliswanga apha. Inkcazo yesiko idibanisa yonke imilinganiselo, idata, imilo, umthamo wevolumu, kunye nolunye ulwazi kwi-JSON. file. I-Opentrons Flex ifunda olu lwazi ukuqonda indlela yokusebenza ngelebhu yesiko lakho. Bona icandelo leeNgcaciso zeLabhu yesiSiko ngolwazi oluthe kratya.
5.6 iibhloko zeAluminiyam
I-Aluminiyam ivala inqanawa kunye neModyuli yoBubushushu be-GEN2 kwaye inokuthengwa ngokwahlukileyo njengeseti yamacandelo amathathu. Iseti ibandakanya ipleyiti esezantsi ephantsi, ibhloko yequla le-24, kunye nebhloko yequla le-96.
I-Opentrons Flex isebenzisa iibhloko ze-aluminiyam ukubamba i-sample iityhubhu kunye neepleyiti kakuhle kwiModyuli yoBubushushu okanye ngqo kwidekhi. Xa isetyenziswe kunye neModyuli yoBubushushu, iibhloko ze-aluminiyam zinokugcina i-s yakhoample iityhubhu, PCR strips, okanye iipleyiti kwiqondo lobushushu rhoqo phakathi 4 °C kunye 95 °C.
Ipleyiti esezantsi ecaba
Ipleyiti esezantsi ethe tyaba yeenqanawa zeFlex ezineCaddy yeModyuli yoBubushushu kwaye iyahambelana neepleyiti zequla eziqhelekileyo zeANSI/SLAS. Eli cwecwe lisicaba liyohluka kwipleyiti ethunyelwa ngeModyuli yoBubushushu ngokwayo okanye iseti enamacandelo amathathu ahlukeneyo. Ibonisa indawo yokusebenza ebanzi kunye neekliphu zekona ezichamfelweyo. Ezi mpawu zinceda ukuphucula ukusebenza kwe-Opentrons Flex Gripper xa uhambisa ilebhu phezu okanye ngaphandle kwepleyiti.
Unokwazi ukuba yeyiphi ipleyiti esezantsi ethe tyaba onayo ngenxa yokuba eyeFlex inamagama athi “Opentrons Flex” kumphezulu wayo. Le ye-OT-2 ayifuni.

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE

Ibhloko ye-aluminiyam engama-24
Ibhloko enamaqula angama-24 isetyenziswa nomntu ngamnyeample izitya. Umzekeloample, yamkela u-sample izitya ukuba:
Yiba namazantsi amile okwe-V okanye amile okwe-U. Khusela imixholo nge-snap cap okanye ukuvalwa kwe-screw cap. Bamba ulwelo ngomthamo we-0.5 mL, 1.5 mL, kunye ne-2 mL.

Ibhloko ye-aluminiyam engama-96
Ibhloko enamaqula angama-96 ixhasa iintlobo ngeentlobo zeepleyiti zequla. Umzekeloample, yamkela kakuhle iipleyiti ezi:
Ukusuka kubavelisi abakhulu bepleyiti entle njengeBio-Rad kunye neNEST.
Idizayinwe ngamazantsi amile okwe-V, amazantsi amile okwe-U, okanye amazantsi asicaba.
Iyilwe nge-100 µL okanye 200 µL yamaqula.
Iyahambelana nemicu yePCR eqhelekileyo.

Iiadaptha ezizimeleyo
Thermal block Flex flat ezantsi ipleyiti 24-well aluminiyam block 96-well aluminiyam block

Igama lomthwalo we-API opentrons_aluminum_flat_bottom_plate Bona indibaniselwano ilebhu engezantsi. opentrons_96_well_aluminiyam_block

Imidibaniso yelebhu yebloko yeAluminiyam
Ithala leencwadi le-Opentrons Labware lixhasa ibhloko elandelayo, ivial, kunye nemidibaniso yepleyiti kakuhle, ekwachazwa kwingcaciso eyahlukileyo yelebhu ye-JSON. files. Irobhothi yeFlex kunye ne-Opentrons Python API

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE
thembela kwezi nkcazelo ze-JSON ukusebenza ngelebhu esetyenziswa ziiprothokholi zakho. Umzekeloample, xa usebenza nge API, i ProtocolContext.load_labware umsebenzi wamkela la magama alebhu njenge parameters ezisebenzayo kwikhowudi yakho. Ezi theyibhile zingezantsi zidwelisa ibhloko engagqibekanga/isikhongozeli indibaniselwano kunye namagama omthwalo we-API anxulumeneyo. Amakhonkco aqhagamshela kwiinkcazo ze-JSON ezihambelanayo kwindawo yokugcina i-Opentrons GitHub.
Qaphela: Zonke iityhubhu zinemiphantsi emile okwe-V ngaphandle kokuba kuboniswe ngenye indlela.

24-kakuhle aluminiyam block labware indibaniselwano

Imixholo yebhloko eyi-24-yemithombo yeGeneric 2 mL screw cap NEST 0.5 mL screw cap NEST 1.5 mL screw cap NEST 1.5 mL snap cap NEST 2 mL screw cap NEST 2 mL snap cap, U-shaped snap cap.

Igama lomthwalo we-API opentrons_24_aluminumblock_generic_2ml_screwcap opentrons_24_aluminumblock_nest_0.5ml_screwcap opentrons_24_aluminumblock_nest_1.5ml_screwcap opentrons_24_aluminumblock_nest_1.5mlcaptrow_screw24 ns_2_aluminiyamblock_nest_24ml_snapcap

96-kakuhle aluminiyam block labware indibaniselwano

Imixholo yebhloko ye-96 ye-Bio-Rad kakuhle ipleyiti 200 µL Generic PCR strip 200 µL NEST ipleyiti entle 100 µL

Igama lomthwalo we-API opentrons_96_aluminumblock_biorad_wellplate_200uL opentrons_96_aluminumblock_generic_pcr_strip_200uL opentrons_96_aluminumblock_nest_wellplate_100uL

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE

5.7 ILabware kunye ne-Opentrons Flex Gripper
Nangona i-Opentrons Flex isebenza nalo lonke uluhlu lweempahla kwiLayibrari yeLabware, i-Opentrons Flex Gripper iyahambelana nezinto ezithile zelebhu kuphela. Okwangoku, i-Gripper ilungiselelwe ukusetyenziswa kunye nezinto ezilandelayo zelebhu.

Udidi lweLabware IiPlati eziNgxaki ziSkirt Ngokugcweleyo ezingama-96 iiPleti eziMhle
IiRakhi zeengcebiso (iingcebiso ezingahluzwanga kunye nezihluziweyo)

Iimpawu
I-NEST 96 Icwecwe eliNzulu nzulu 2 mL
Opentrons Tough 96 Well Plate 200 µL PCR Full Skirt NEST 96 Well Plate 200 µL Flat
Opentrons Flex 96 Tip Rack 50 µL Opentrons Flex 96 Tip Rack 200 µL Opentrons Flex 96 Tip Rack 1000 µL

Qaphela: Ukuze ufumane iziphumo ezingcono, sebenzisa iFlex Gripper kuphela ilebhu edweliswe ngasentla. I-Flex Gripper inokusebenza kunye ne-ANSI/SLAS i-automation labware ethobelayo, kodwa oku akukhuthazwa.

5.8 Iinkcazo zelebhu eqhelekileyo
Njengoko kuxoxiwe ekuqaleni kwesi sahluko, ilebhu yesiko yilabware engadweliswanga kwithala leencwadi leOpentrons Labware. Ungasebenzisa ezinye izinto eziqhelekileyo okanye ezizodwa zelebhu kunye neFlex ngokulinganisa ngokuchanekileyo kunye nokurekhoda iimpawu zaloo nto kwaye ugcine loo datha kwi-JSON. file. Xa ingeniswa kwi-app, i-Flex kunye ne-API isebenzisa loo datha ye-JSON ukusebenzisana ne-labware yakho. I-Opentrons ibonelela ngezixhobo kunye neenkonzo, esiza kuziqwalasela ngezantsi, ukukunceda ukuba usebenzise iFlex ngeelebhu yesiko.

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE

Ukudala iinkcazo zelebhu yesiko
Izixhobo kunye neenkonzo ze-Opentrons zinceda ukubeka ilebhu yesiko ekufikeleleni kwakho. Ezi mpawu zibandakanya amanqanaba ezakhono ezahlukeneyo kunye neendlela zokusebenza. Ukuzenzela eyakho ilebhu, kwaye uyisebenzise nge-Opentrons Flex, inceda ukwenza irobhothi yongezelelo oluguquguqukayo nolunamandla kwilebhu yakho.

UMDALI WEMSEBENZI YESIKO
Umdali weLebhu yesiNtu asiyokhowudi, web-Isixhobo esisekwe esisebenzisa ujongano lomzobo ukukunceda wenze inkcazelo yelebhu file. Umdali weLabware uvelisa inkcazo yelebhu ye-JSON file oyingenisayo kwi-Opentrons App. Emva koko, i-labware yakho yesiko iyafumaneka kwi-robot ye-Flex kunye ne-Python API.

INKONZO YOMTHETHO WESIKO
Qhagamshelana nathi ukuba ilebhu ongathanda ukuyisebenzisa ayifumaneki kwithala leencwadi, ukuba awukwazi ukuyila iinkcazelo zakho, okanye ngenxa yokuba into eqhelekileyo ibandakanya iimilo ezahlukeneyo, ubungakanani, okanye ezinye izitenxo ezichazwe ngezantsi.

I-Labware ungayichaza kwi-Labware Creator
; Imithombo kunye neetyhubhu zifana kwaye ziyafana. ; Yonke imiqolo ibekwe ngokulinganayo
(isithuba phakathi kwemiqolo siyalingana).
; Zonke iikholamu zibekwe ngokulinganayo (isithuba phakathi kweekholamu siyalingana).
; Ingena ngokugqibeleleyo kwindawo yedekhi enye.

ILabware Opentrons kufuneka ichaze; Imithombo kunye neemilo zetyhubhu ziyahluka. ; Imiqolo ayibekwanga ngokulinganayo.
; Iikholamu azichasananga ngokulinganayo.
; Incinci kunendawo yokubeka idekhi enye (ifuna iadaptha) okanye ivuleke kwiindawo zokubeka ezininzi.

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE
Nantsi eminye imizobo ekunceda ukuba ube nombono we-exampichazwe ngasentla. Rhoqo Yonke imiqolo ibekwe ngokulinganayo kwaye yonke imiqolo ibekwe ngokulinganayo. Iikholamu akufuneki zibe nezithuba ezifanayo njengemiqolo.
Rhoqo Igridi ayinyanzelekanga ukuba ibe sembindini welebhu.
Imiqolo engaqhelekanga ibekwe ngokulinganayo kodwa iikholamu azishiywanga ngokulinganayo.
Iikholamu/imiqolo engaqhelekanga ibekwe ngokulinganayo kodwa amaqula awafani.
Ngokungaqhelekanga Kukho ngaphezu kwegridi enye.
Iqela lethu lelebhu liya kusebenza ukuqonda iimfuno zakho kunye nokuyila iinkcazo zelebhu yesiko kuwe. Jonga amanqaku enkxaso ecela iNgcaciso yeLabhu yeSiko kunye nefomu yeSicelo seLabhure yeSiko ngolwazi oluthe kratya. Le yinkonzo esekelwe kwintlawulo.

I-OPENTRONS FLEX

ISAHLUKO SESI-5: I-LABWARE

PYTHON API
Ngelixa awukwazi ukwenza ilebhu yesiko nge-API yethu, ungasebenzisa ilebhu yesiko ngeendlela ezikhoyo ze-API. Nangona kunjalo, kufuneka uchaze ilebhu yesiko lakho kuqala kwaye uyingenise kwi-Opentrons App. Nje ukuba wongeze ilebhu yakho kwi-Opentrons App, iyafumaneka kwiPython API kunye nerobhothi. Jonga icandelo leeNgcaciso zeLabhu yeSiko lePython API amaxwebhu ngolwazi olungakumbi. Ngolwazi malunga nokubhala izikripthi zeprotocol kunye ne-API, bona icandelo lePython Protocol API kwisahluko soPhuhliso lweProtocol.
I-JSON labware schema
A JSON file yiplani yeOpentrons esemgangathweni kunye nelebhu yesiko. Oku file iqulathe kwaye iququzelele idatha yelebhu ngokwemigaqo yoyilo ebekwe yi-schema engagqibekanga.
I-schema isakhelo

Amaxwebhu / Izibonelelo

opentrons FLEX FLEX Opentrons Flex Open Source Liquid Handling Robot [pdf] Incwadi Yomyalelo
I-FLEX Opentrons Flex Umthombo oVulelekileyo wokuPhathwa kweRobhothi, iFLEX, iOpentrons Flex eVulelekileyo yoMthombo woLawulo lweRobhothi, iFlex eVulelekileyo yokuHamba iRobhothi, iRobhothi yokuphatha ulwelo, iRobhothi yokuphatha ulwelo, iRobhothi yokuphatha ulwelo, iRobhothi yokuphatha,

Iimbekiselo

Incwadi yokusebenzisa

FLEX Opentrons Flex Open Source Liquid Handling Robot

I-Opentrons Flex

Iinkcukacha:

Imiyalelo yokusetyenziswa kwemveliso:

1. UFakelo lwesixhobo kunye noLungiso:

2. Ukufuduswa:

3. Uqhagamshelwano:

4. Umyili weProtocol:

5. IPython Protocol API:

6. IiProthokholi ze-OT-2:

Imibuzo Ebuzwa Rhoqo (FAQ):

Umbuzo: Ndiyicombulula njani ingxaki ukuba irobhothi ayihambi njenge
kulindeleke?

Umbuzo: Ndingasebenzisa iipipettes zesiko kunye ne-Opentrons Flex?

Amaxwebhu / Izibonelelo

Iimbekiselo

Shiya uluvo

Rhoxisa impendulo

FLEX Opentrons Flex Open Source Liquid Handling Robot

I-Opentrons Flex

Iinkcukacha:

Imiyalelo yokusetyenziswa kwemveliso:

1. UFakelo lwesixhobo kunye noLungiso:

2. Ukufuduswa:

3. Uqhagamshelwano:

4. Umyili weProtocol:

5. IPython Protocol API:

6. IiProthokholi ze-OT-2:

Imibuzo Ebuzwa Rhoqo (FAQ):

Umbuzo: Ndiyicombulula njani ingxaki ukuba irobhothi ayihambi njenge kulindeleke?

Umbuzo: Ndingasebenzisa iipipettes zesiko kunye ne-Opentrons Flex?

Amaxwebhu / Izibonelelo

Iimbekiselo

Izithuba eziyeleleneyo

Shiya uluvo

Rhoxisa impendulo

Umbuzo: Ndiyicombulula njani ingxaki ukuba irobhothi ayihambi njenge
kulindeleke?