Gabaɗaya Bayani: Lorem ipsum dolor zauna
amet, consectetur adipiscing elit. Sed don eiusmod tempor inciddunt
ut labour and dolore magna aliqua.
Ƙayyadaddun Muhalli: Lorem ipsum
dolor zauna amet, consectetur adipiscing elit. Za a yi eiusmod tempor
inciddunt u labore et dolore magna aliqua.

Takaddun shaida: Lorem ipsum dolor zauna amet,
consectetur adipiscing elit. Sed do eiusmod tempor inciddunt ut
labour et dolore magna aliqua.
Serial Number: XXX-XXX-XXX

Umarnin Amfani da samfur:

1. Shigarwa da Daidaita Kayan aiki:

Bi matakan da aka zayyana a cikin jagorar pipette da gripper
shigarwa.

2. Matsar:

Don gajerun motsi, koma zuwa sashe na 2.5 a cikin jagorar. Domin
tafiya mai nisa, bi ƙa'idodin da aka bayar. Gabaɗaya motsi
shawara kuma akwai.

3. Haɗi:

Tabbatar da haɗin wutar lantarki daidai kamar yadda cikakken bayani a cikin jagorar.
Haɗa USB da na'urori masu taimako kamar yadda ake buƙata. Hanyoyin sadarwa
ya kamata a kafa bin umarnin.

4. Mai tsara yarjejeniya:

Fahimtar buƙatun don Mai tsara yarjejeniya kuma koyi yadda
don ƙirƙira sabbin ka'idoji ko gyara waɗanda ke akwai kamar yadda yake a cikin dakin binciken ku
bukatun.

5. Python Protocol API:

Bincika rubutu da gudanar da rubutun ta amfani da Python Protocol API.
Gano abubuwan musamman na Python don ingantattun ayyuka.

6. OT-2 ladabi:

Koyi game da OT-2 Python ladabi, OT-2 JSON ladabi, da
Ka'idojin Module Magnetic don nau'ikan gwaje-gwaje daban-daban.

Tambayoyin da ake yawan yi (FAQ):

Tambaya: Ta yaya zan magance matsalar idan robot baya motsi kamar
sa ran?

A: Bincika haɗin wutar lantarki, tabbatar da daidaitaccen daidaitawar
kayan aiki, da kuma tabbatar da cewa babu cikas a cikin
hanyar robot.

Tambaya: Zan iya amfani da pipettes na al'ada tare da Opentrons Flex?

A: Opentrons yana ba da shawarar amfani da pipettes masu jituwa don mafi kyau
aiki da daidaito.

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Opentrons Flex
Jagoran Jagora
Opentrons Labworks Inc. girma
Disamba 2023

© OPENTRONS 2023 Opentrons FlexTM (Opentrons Labworks, Inc.) Sunaye masu rijista, alamun kasuwanci, da sauransu. da aka yi amfani da su a cikin wannan takaddar, koda lokacin da ba a yi musu alama ta musamman ba, ba za a ɗauke su da rashin kariyar doka ba.

Teburin Abubuwan Ciki
Gabatarwa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 Tsarin wannan littafin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Bayanan kula da gargadi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Babi na 1: Gabatarwa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 1.1 Barka da zuwa Opentrons Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Menene sabo a Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Flex workstations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 1.2 Bayanin aminci. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Alamun aminci. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Gargadi na aminci na lantarki. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Ƙarin gargaɗin aminci. . . . . . . . . . . . . . . . . . . . . . . . . . . .18 Tsananin tsaro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 Tsaron Halittu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Turi mai guba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Ruwa masu ƙonewa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 1.3 Biyayya ga tsari. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Tsaro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Daidaitawar lantarki. . . . . . . . . . . . . . . . . . . . . . . . .20 FCC gargadi da bayanin kula. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Kanada ISD yarda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Gargadi na muhalli. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Wi-Fi precertification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Babi na 2: Shigarwa da Matsarwa . . . . . . . . . . . . . . . . . . . . . . . 22 2.1 Tsaro da buƙatun aiki . . . . . . . . . . . . . . . . . . . . .22 Inda za a sanya Opentrons Flex. . . . . . . . . . . . . . . . . . . . . . . .22 Amfani da wutar lantarki. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 Yanayin muhalli. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 2.2 Cire dambe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Ƙoƙari da lokacin da ake buƙata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Akwati da kayan tattarawa. . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Abubuwan samfur . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 Sashe na 1: Cire akwati . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27 Sashe na 2: Saki Saki. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29 Sashe na 3: Taro na ƙarshe da iko akan . . . . . . . . . . . . . . . . . . .32

2.3 Gudun farko . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 A kunne . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Haɗa zuwa cibiyar sadarwa ko kwamfuta . . . . . . . . . . . . . . . . . . . . .36 Shigar da sabunta software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37 Haɗa Tsayawar Gaggawa. . . . . . . . . . . . . . . . . . . . . . 38 Ka ba mutum-mutumin suna. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
2.4 Shigar da kayan aiki da daidaitawa. . . . . . . . . . . . . . . . . 38 Shigar Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 Shigar Gripper. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
2.5 Matsar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 Gajerun motsi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Motsa jiki mai nisa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Gabaɗaya nasiha mai motsi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 Tunani na ƙarshe game da motsi. . . . . . . . . . . . . . . . . . . . . . . . . . .44
Babi na 3: Bayanin Tsarin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 3.1 Abubuwan Jiki . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45 Firam da shinge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 bene da wurin aiki. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 Stagyankin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47 Gilashin bene. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 Sharar gida. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49 Staging area ramummuka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49 Tsarin motsi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 allon taɓawa da nunin LED. . . . . . . . . . . . . . . . . . . . . . . . .51 3.2 Pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 Pipette ƙayyadaddun bayanai. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Gyaran bututu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Adaftar tip tukwici. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Karɓar ɓangaren tip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 Pipette firikwensin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 Sabunta firmware Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 3.3 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 Bayani dalla-dalla. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Gripper calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Gripper firmware updates. . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 3.4 Tsayawa Tsaida Gaggawa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 Lokacin amfani da E-Stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 Shiga da sakin E-Stop. . . . . . . . . . . . . . . . . . . . .60

3.5 Haɗi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 Haɗin wutar lantarki. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 USB da haɗin haɗin gwiwa. . . . . . . . . . . . . . . . . . . . . . . . .62 Hanyoyin sadarwa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
3.6 Bayanin tsarin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63 Gabaɗaya Bayani. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63 Bayanin muhalli. . . . . . . . . . . . . . . . . . . . . . . . . .64 Takaddun shaida. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 Serial number . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64
Babi na 4: Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 4.1 Moduloli masu goyan baya . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66 4.2 Module caddy tsarin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 4.3 Module daidaitawa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68 Lokacin da za a daidaita kayayyaki. . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 Yadda ake daidaita kayayyaki. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 4.4 Module-Shaker mai zafi GEN1 . . . . . . . . . . . . . . . . . . . . . . . . . .70 Mai zafi-Shaker fasali. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70 Bayani dalla-dalla na Heater-Shaker. . . . . . . . . . . . . . . . . . . . . . . . . .72 4.5 Magnetic Block GEN1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 Magnetic Block fasali. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 Magnetic Block ƙayyadaddun bayanai. . . . . . . . . . . . . . . . . . . . . . . . .74 4.6 Zazzabi Module GEN2. . . . . . . . . . . . . . . . . . . . . . . . . . . .75 Yanayin Module Zazzabi . . . . . . . . . . . . . . . . . . . . . . . . .75 Matsakaicin Modulun Zazzabi. . . . . . . . . . . . . . . . . . . .77 4.7 Module na Keke GEN2. . . . . . . . . . . . . . . . . . . . . . . . . . . .77 Fasalolin Thermocycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .78 Bayani dalla-dalla na thermocycler. . . . . . . . . . . . . . . . . . . . . . . . . . .79
Babi na 5: Labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 5.1 Ra'ayoyin Labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Labware azaman hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Labware azaman bayanai . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Custom labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81 5.2 Tafkuna . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Tafkunan rijiyoyin guda daya . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Rijiyoyi masu yawa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Reservoirs da API ma'anar . . . . . . . . . . . . . . . . . . . . . . . . 83 Laburaren tafki na al'ada. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 5.3 Rijiyar faranti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

6-faranti . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 12-rijiya faranti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 24-rijiya faranti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 48-rijiya faranti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 96-rijiya faranti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 384-rijiya faranti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 To farantin adaftan. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 Well faranti da API ma'anar. . . . . . . . . . . . . . . . . . . . . . . . .87 Laburaren rijiyar al'ada. . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 5.4 Nasihu da tukwici. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 Tukwici. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 Daidaituwar Tippipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 Adaftar tukwici. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 5.5 Tubus da bututu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90 Tube da tarawa haduwa. . . . . . . . . . . . . . . . . . . . . . . . . . . 91 6-tubu tara. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 10-tube tara. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 15-tubu tara. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 24-tubu tara. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Tube rack API ma'anar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Custom tube tara labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 5.6 Aluminum tubalan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 Flat kasa farantin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 24- rijiyar aluminum block. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 96- rijiyar aluminum block. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Adaftan tsaye . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Aluminum block labware haduwa. . . . . . . . . . . . . . . .94 24- rijiyar aluminium block labware haduwa. . . . . . . . .95 96- rijiyar aluminium block labware haduwa. . . . . . . . .95 5.7 Labware da Opentrons Flex Gripper. . . . . . . . . . . . . . .96 5.8 Ma'anonin labware na al'ada. . . . . . . . . . . . . . . . . . . . . . . . . . . .96 Ƙirƙirar ma'anar labware na al'ada. . . . . . . . . . . . . . . . . . .97 JSON tsarin labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99 JSON ma'anar labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104 Babi na 6: Ci gaban yarjejeniya. . . . . . . . . . . . . . . . . . . . . . . . . . .105 6.1 Ka'idojin da aka riga aka yi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105 Laburaren yarjejeniya. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105 Custom Protocol Development sabis. . . . . . . . . . . . . . . .

6.2 Mai tsara yarjejeniya. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109 Bukatun Mai tsara yarjejeniya. . . . . . . . . . . . . . . . . . . . . . 109 Zayyana yarjejeniya. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Gyara ƙa'idodin da ke akwai. . . . . . . . . . . . . . . . . . . . . . . . . . 114
6.3 Python Protocol API . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 Rubutun rubutu da gudana. . . . . . . . . . . . . . . . . . . . . . . . . . . 115 Python-keɓaɓɓun fasali. . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
6.4 OT-2 ladabi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117 OT-2 Python ladabi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118 OT-2 JSON ladabi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120 Magnetic Module ladabi. . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Babi na 7: Software da Aiki. . . . . . . . . . . . . . . . . . . . . . . . . . 121 7.1 Aikin allo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 Robot dashboard. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 Gudanar da yarjejeniya. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122 cikakkun bayanai na yarjejeniya. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124 Run saitin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127 Duba Matsayin Labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128 Gudu ci gaba. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130 Gudu kammala. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131 Gudanar da kayan aiki. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132 Robot saituna. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Tsarin bene na 133. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136 7.2 Opentrons App. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 App shigarwa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 Canja wurin ladabi zuwa Flex. . . . . . . . . . . . . . . . . . . . . . . . . Matsayin Module da sarrafawa 140. . . . . . . . . . . . . . . . . . . . . . . . . . . 142 Kwanan nan yarjejeniya yana gudana . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143 7.3 Babban aiki. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144 Jupyter Notebook. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144 Ayyukan layin umarni akan SSH. . . . . . . . . . . . . . . . . . . . 145
Babi na 8: Kulawa da Hidima . . . . . . . . . . . . . . . . . . . . . . . .147 8.1 Tsaftace Matsala . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Kafin ka fara . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Abin da za ku iya tsaftacewa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 tsaftacewa mafita. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148 Frame da tsabtace panel taga. . . . . . . . . . . . . . . . . . . . 148 Tsabtace bene. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148

Gantry tsaftacewa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 shara shara. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 8.2 Tsabtace pipettes da tukwici . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 lalata Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150 Tsabtace pipette tukwici. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151 8.3 Tsaftace abin riko . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152 8.4 Tsabtace kayayyaki. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 Babban tsarin tsaftacewa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 Thermocycler hatimi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 8.5 Autoclave-amintaccen labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 8.6 Sabis na Sabis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 Opentrons sabis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 cancantar shigarwa da cancantar aiki. . . 155 Kulawa na hanawa . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156 Garanti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157 Karin Bayani A: Kalmomi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .158 Karin Bayani B: Ƙarin Takardun . . . . . . . . . . . . . . . . . . . . . .170 B.1 Opentrons Knowledge Hub. . . . . . . . . . . . . . . . . . . . . . . . . . . . 170 B.2 Python Protocol API takardun. . . . . . . . . . . . . . . . . . . 170 B.3 Opentrons HTTP API tunani. . . . . . . . . . . . . . . . . . . . . . . . 171 B.4 Takardun masu haɓakawa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171 Karin bayani C: Buɗe-Source Software. . . . . . . . . . . . . . . . . . . . . . . . .172 C.1 Opentrons akan GitHub . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172 C.2 Opentrons monorepo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173 C.3 Sauran wuraren ajiya. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175 Karin Bayani D: Taimako da Bayanin Tuntuɓi . . . . . . . . . . . . . . .176 D.1 Talla. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 D.2 Taimako . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 D.3 Bayanan kasuwanci. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177

Gabatarwa
Barka da zuwa ga jagorar koyarwa na Opentrons Flex robot mai sarrafa ruwa. Wannan jagorar tana jagorantar ku ta kusan duk abin da kuke buƙatar sani don saitawa da amfani da Flex, yana mai da hankali kan batutuwan da suka fi dacewa ga masu amfani da Flex na yau da kullun a cikin yanayin lab.
Tsarin wannan littafin
Opentrons Flex tsari ne mai rikitarwa, don haka akwai hanyoyi daban-daban don koyan duk abin da zai iya yi. Jin kyauta don tsalle kai tsaye zuwa babin da ke magana akan kowane batun da kuke sha'awar! Don misaliampto, idan kun riga kuna da Flex da aka saita a cikin lab ɗin ku, zaku iya tsallake babin Shigarwa da Matsarwa.
Idan kun fi son hanyar jagora, wannan jagorar an tsara shi don ku iya bi ta daga farko zuwa ƙarshe.
Koyi game da Flex. An jera abubuwan banbance-banbance na Flex a Babi na 1: Gabatarwa. Gabatarwa kuma ta ƙunshi mahimman bayanai na aminci da tsari.
Fara da Flex. Idan kana buƙatar saita Flex ɗin ku, bi cikakkun bayanai a Babi na 2: Shigarwa da Matsarwa. Sannan san kanku da abubuwan da ke cikin Flex a Babi na 3: Siffar Tsari.
Saita benen ku. Saita bene yana ba da damar aikace-aikacen kimiyya daban-daban akan Flex. Babi na 4: Modules sun bayyana abubuwan da ke kewaye da Opentrons waɗanda za ku iya shigar a ciki ko a saman bene don yin takamaiman ayyukan kimiyya. Babi na 5: Labware yana bayanin yadda ake aiki da kayan aiki don riƙe ruwa.
Gudanar da yarjejeniya. Babban amfani da Flex yana gudanar da daidaitattun hanyoyin kimiyya, waɗanda aka sani da ladabi. Babi na 6: Ci gaban yarjejeniya yana ba da hanyoyi da yawa don samun shirye-shiryen ladabi ko tsara su da kanku. Don gudanar da ƙa'idar ku, bi umarni a Babi na 7: Software da Aiki, wanda kuma yana da umarnin yin wasu ayyuka da tsara saitunan robot ɗin ku.
Ci gaba da gudana Flex. Bi shawara a Babi na 8: Kulawa da Sabis don kiyaye Flex ɗinku mai tsabta da aiki da kyau. Ko yin rajista don ɗayan ayyukan Opentrons da aka jera a wurin kuma bari mu kula da ku Flex.
Koyi har ma. Har yanzu kuna buƙatar wani abu dabam? Tuntuɓi appendices. Karin bayani A: Kalma yana bayyana sharuddan da ke da alaƙa da Flex. Shafi B: Ƙarin Takaddun shaida yana nuna maka har ma da ƙarin albarkatu don samfuran Opentrons da lambar rubutu don sarrafa Flex.

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GABATARWA
Karin bayani C: Buɗe-Source Software yana bayanin yadda Opentrons software ke ɗaukar nauyin GitHub a matsayin hanya ga masu haɓakawa da waɗanda ba masu haɓakawa ba.
Shafi D: Taimako da Bayanan Tuntuɓi ya lissafa yadda ake tuntuɓar Opentrons idan kuna buƙatar taimako fiye da abin da takaddunmu ke bayarwa.
Bayanan kula da gargadi
A cikin wannan jagorar, zaku sami ingantaccen bayanin kula da tubalan faɗakarwa. Bayanan kula suna ba da bayanai masu taimako waɗanda ƙila ba za su fito fili ba a cikin tsarin yau da kullun na amfani da Flex. Kula da hankali na musamman ga gargaɗi-ana amfani da su ne kawai a cikin yanayin da kuke fuskantar haɗarin rauni na mutum, lalacewar kayan aiki, asara ko ɓarna na s.amples ko reagents, asarar bayanai, ko wasu lahani. Bayanan kula da gargadi sunyi kama da haka:
SampLura: Wannan abu ne da ya kamata ku sani, amma ba ya haifar da wani haɗari.
SampGargaɗi: Wannan wani abu ne da kuke buƙatar sani saboda akwai haɗarin da ke tattare da shi.

OPENTRONS FLEX

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Gabatarwa

Wannan babin yana gabatar muku da tsarin yanayin yanayin Opentrons Flex, gami da ƙirar tsarin gabaɗaya da kuma saitunan wurin aiki. Hakanan ya haɗa da mahimman yarda da bayanan aminci, waɗanda yakamata ku sake suview kafin kafa robot na Opentrons Flex. Don ƙarin cikakkun bayanai kan fasalulluka na Opentrons Flex, duba babin Bayanin Tsarin.

1.1 Barka da zuwa Opentrons Flex
Opentrons Flex mutum-mutumi ne mai sarrafa ruwa wanda aka ƙera don babban kayan aiki da hadaddun ayyukan aiki. Robot ɗin Flex shine tushen tsarin na'ura wanda ya haɗa da pipettes, gripper na labware, kayan aikin bene, na'urorin kan bene, da labware - duk waɗanda zaku iya musanya da kanku. An ƙera Flex tare da allon taɓawa don ku iya aiki da shi kai tsaye a benci na lab, ko kuna iya sarrafa shi daga ko'ina cikin lab ɗin ku tare da Opentrons App ko APIs masu buɗewa.
Wuraren aiki na Flex sun zo tare da duk kayan aiki - robot, hardware, da labware - waɗanda kuke buƙatar fara sarrafa ayyukan laburar gama gari. Ga wasu aikace-aikacen, Opentrons Flex yana aiki akan cikakkiyar buɗaɗɗen software da firmware, kuma shine reagent- da labware-agnostic, yana ba ku iko akan yadda kuke ƙira da gudanar da ka'idojin ku.

Menene sabo a Flex
Opentrons Flex wani bangare ne na jerin robobin masu sarrafa ruwa na Opentrons. Masu amfani da Opentrons Flex ƙila sun saba da Opentrons OT-2, mutummutumi na bututun mu. Flex ya wuce ƙarfin OT-2 a wurare masu mahimmanci, yana ba da mafi girman kayan aiki da lokacin tafiya.

Feature kayan aikin Pipette
Pipette da iyawar tip

Bayani
Flex pipettes suna da tashoshi 1, 8, ko 96. Pipette mai tashar tashoshi 96 yana aiki a kan rijiyoyin da yawa sau 12 a lokaci ɗaya kamar pipette mafi girma na OT-2.
Flex pipettes suna da girma girma jeri (1 µL, 50 µL) kuma duk suna iya aiki tare da kowane girma na Opentrons Flex tukwici. Wannan haɓakawa ne akan pipettes OT-5, waɗanda ke da ƙananan jeri kuma dole ne suyi amfani da tukwici tare da kewayon ƙarar daidai.

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BABI NA 1: GABATARWA

Gripper Automated Calibration Touchscreen Module caddies Deck Ramin daidaitawa Girman sharar Motsi da nauyi

Opentrons Flex Gripper yana ɗauka yana motsa labware a kusa da bene ta atomatik, ba tare da sa hannun mai amfani ba. Mai riko yana ba da ƙarin hadaddun ayyukan aiki a cikin tsari guda ɗaya.
Matsakaicin daidaitawar pipettes na Flex da gripper cikakke ne mai sarrafa kansa. Latsa maɓalli ɗaya, kuma kayan aikin zai matsa zuwa madaidaicin mashin ɗin akan bene don tantance ainihin matsayinsa, adana bayanan don amfani a cikin ladabi.
Flex yana da nasa allon taɓawa wanda zai baka damar sarrafa shi kai tsaye, ban da amfani da Opentrons App. Yi amfani da allon taɓawa don fara ƙa'idar aiki, duba matsayin aiki, da canza saituna daidai akan robot.
Modulolin Flex sun dace cikin ɗimbin kadi waɗanda ke mamaye sarari a ƙasan bene. Caddies suna sanya labware ɗin ku kusa da saman bene kuma suna ba da izinin kewaya kebul na ƙasa. Caddies yana ba da damar ƙarin ƙirar ƙira da saitunan labware akan bene.
Ramin bene akan Flex ana ƙididdige su tare da tsarin daidaitawa (A1D4) wanda yayi kama da yadda ake ƙidayar rijiyoyi akan labware.
Wurin shara na iya tafiya a wurare da yawa a kan Flex. Wurin tsoho (ramin A3) shine matsayin da aka ba da shawarar. Hakanan zaka iya amfani da gripper don zubar da shara a cikin sharar zaɓin zaɓi.
Flex ya ɗan girma kuma ya fi OT-2 nauyi. Ayyukan shigarwa akan Flex suna buƙatar taimakon abokin aikin lab.

Ana samun cikakken kwatancen ƙayyadaddun fasaha na mutum-mutumi akan Opentrons website.
Duka Flex da OT-2 mutum-mutumi suna aiki akan software na buɗe tushen mu, kuma Opentrons App na iya sarrafa nau'ikan robots guda biyu a lokaci ɗaya. Yayin da OT-2 ba za a iya tafiyar da ka'idojin OT-2 kai tsaye akan Flex ba, yana da sauƙi don daidaita su (duba sashin ladabi na OT-XNUMX na babin Ci gaban yarjejeniya don cikakkun bayanai).

Matsalolin aiki
Opentrons Flex ayyuka sun haɗa da robot Flex, na'urorin haɗi, pipettes da gripper, na'urorin kan bene, da labware da ake buƙata don sarrafa takamaiman aikace-aikacen. Duk abubuwan da aka gyara wurin aiki na zamani ne. Idan kana buƙatar canza aikace-aikace, za ka iya ƙara ko musanya a cikin wasu kayan aikin Flex da masu jituwa masu jituwa.

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Farashin NGS
Opentrons Flex NGS Workstation yana sarrafa shirye-shiryen ɗakin karatu na NGS. Yana iya sarrafa kansa pre-sequencing workflows ta amfani da kowane manyan reagent tsarin, ciki har da fragmentation- da tagPrep na tushen laburare.
Baya ga robot Flex, NGS Workstation ya haɗa da:
Gripper Zaɓin daidaitawar pipette
Biyu 8-Channel Pipettes (1 µL da 50 µL) 5-Channel Pipette (1000 µL) Waste Chute Magnetic Block Temperatuur Module Thermocycler Module Labware kit tare da tace tukwici, microcentrifuge tubes, reservoirs, da PCR.
PCR AIKI
Opentrons Flex PCR Workstation yana sarrafa saitin PCR da ayyukan aikin thermocycling har zuwa 96 samples. Yana iya fitar da reagents masu sanyi da samples cikin farantin PCR mai rijiyar 96. Tare da ƙarin Module na Thermocycler mai sarrafa kansa, yi amfani da gripper don loda farantin cikin Thermocycler, sannan gudanar da shirin PCR da kuka zaɓa.
Baya ga robot Flex, PCR Workstation ya haɗa da:
Gripper Zaɓin daidaitawar pipette
1-Channel Pipette (1 µL) da 50-Channel Pipette (8 µL) 1-Channel Pipette (50 µL) Waste Chute Temperatuur Module Labware Kit tare da tukwici na tacewa, microcentrifuge tubes, reservoirs, da PCR faranti.

OPENTRONS FLEX

BABI NA 1: GABATARWA
AIKIN HARIN NUCLEIC ACIID
Aikin Opentrons Flex Nucleic Acid Extraction Workstation yana sarrafa keɓewar DNA/RNA da tsarkakewa. Yana amfani da Magnetic Block don rabuwa da beads na maganadisu, da Heater-Shaker don s.ample lysis da resuspension na Magnetic beads.
Baya ga Robot Flex, Aikin Haɗin Acid Nucleic Acid ya haɗa da:
Gripper Zaɓin daidaitawar pipette
1-Channel Pipette (5 µL) da 1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL) Waste Chute Magnetic Block Heater-Shaker Module Labware kit tare da tace tukwici, tafki, PCR faranti, da zurfi faranti, da kuma zurfin PCR faranti, da kuma zurfin faranti.
MAGANAR BEAD PROTEIN TSARKI AIKIN
The Opentrons Flex Magnetic Bead Protein Worksification Worksation yana sarrafa ƙananan sikelin furotin da kuma proteomics s.ample prep har zuwa 96 samples. Ya dace da yawancin mashahurin reagents na tushen maganadisu.
Baya ga Robot Flex, Wurin Tsabtace Protein ya haɗa da:
Gripper Zaɓin daidaitawar pipette
1-Channel Pipette (5 µL) da 1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL) Waste Chute Magnetic Block Heater-Shaker Module Labware kit tare da tace tukwici, tafki, PCR faranti, da zurfi faranti, da kuma zurfin PCR faranti, da kuma zurfin faranti.
FLEX PREP AIKI
The Opentrons Flex Prep Workstation yana sarrafa sauƙin aikin bututun aiki. Saita wurin aiki tare da 1-Channel da 8-Channel Pipettes don aiwatar da ayyuka kamar s.ampcanja wuri, sample kwafi, da

OPENTRONS FLEX

BABI NA 1: GABATARWA
reagent alkalami. Saita wurin aiki tare da 96-Channel Pipette don yin babban kayan aikin reagent aliquoting da farantin karfe st.amping.
Baya ga robot Flex, Flex Prep Workstation ya haɗa da:
Zaɓin daidaitawar pipette: 1-Channel Pipette (5 µL) da 1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL)
Kayan aikin Labware tare da tukwici masu tacewa, bututun microcentrifuge, da tafki
PLASMID PREP AIKI
The Opentrons Flex Plasmid Prep Workstation yana sarrafa aikin haƙar plasmid na tushen maganadisu da aikin tsarkakewa. Wannan wurin aiki an sanye shi da pipettes masu girma, Module mai zafi-Shaker, da Magnetic Block don ɗaukar mafi yawan sinadarai na tushen katako.
Baya ga robot Flex, Plasmid Prep Workstation ya haɗa da:
Gripper 1-Channel Pipette (5 µL) da 1000-Channel Pipette (8 µL) Waste Chute Magnetic Block Heater-Shaker Module Labware Kit tare da tukwici na tacewa, bututun microcentrifuge, tafki, faranti PCR, da faranti mai zurfi.
SYNBIO AIKI
The Opentrons Flex SynBio Workstation yana sarrafa nau'ikan ayyukan aikin ilimin halitta na roba kamar haɗin DNA da cloning. Yana amfani da Magnetic Block da Module Zazzabi don tallafawa mafi yawan sinadarai na tushen katako. Ƙara Module na Thermocycler don yin zafafan murfi da ampillolin.
Baya ga robot Flex, SynBio Workstation ya haɗa da:
Gripper 1-Channel Pipette (5 µL) da 1000-Channel Pipette (8 µL) Magnetic Block Temperature Module

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BABI NA 1: GABATARWA

Kayan aikin Labware tare da tukwici na yau da kullun da tacewa, bututun microcentrifuge, tafki, faranti PCR, da faranti mai zurfi.

1.2 Bayanin aminci
An ƙera na'urar sarrafa ruwa ta Opentrons Flex don aiki mai aminci. Koma zuwa ƙayyadaddun ƙayyadaddun ƙa'idodi da ƙa'idodin yarda a cikin wannan sashe don tabbatar da amintaccen amfani da Flex ɗin ku. Waɗannan jagororin sun ƙunshi amintaccen amfani da haɗin shigarwa da fitarwa don samfurin, gami da ƙarfi da haɗin bayanai, da kuma alamun gargaɗin da aka samu akan Flex robot da kayan aikin da ke da alaƙa. Yin amfani da na'urar ta wata hanya dabam da waɗanda aka ƙayyade a cikin wannan jagorar na iya jefa mai amfani da kayan aiki cikin haɗari.

Alamun aminci
Alamomi daban-daban akan Flex kuma a cikin wannan jagorar suna yi muku gargaɗi game da tushen yuwuwar rauni ko lahani.

Alama

Bayani
Gargaɗi: Yana faɗakar da masu amfani zuwa ga yuwuwar yanayi masu haɗari. Ayyukan da zasu iya haifar da rauni ko mutuwa.
Tsanaki: Gargaɗi masu amfani game da lalacewar kayan aiki. Batattu ko gurɓatattun bayanai. Katsewar aikin da ba a iya murmurewa ba.
Girgizar Wutar Lantarki: Yana gano abubuwan kayan aiki waɗanda zasu haifar da haɗarin girgiza wutar lantarki idan kayan aikin ba su da kyau.

Fuskar zafi: Yana gano abubuwan kayan aiki waɗanda ke haifar da haɗarin rauni na mutum saboda tsananin zafi/zazzabi idan an sarrafa kayan aikin ba daidai ba.

Maƙallin Tsoka: Yana gano abubuwan kayan aiki waɗanda zasu iya haifar da haɗarin rauni na mutum yayin motsi.

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BABI NA 1: GABATARWA

Za ku sami alamun masu zuwa akan Flex:
Alamomin mallakar fasaha Alamomin bin ka'ida (misali, ETL) Lakabin haɗari na lantarki Gabaɗayan alamun faɗakarwa Alamomin samfur Takamaiman mahimmi mai girma vol.tage Labels Lakabin Ƙimar Ƙarfi

Gargadi na aminci na lantarki
Koyaushe kiyaye gargaɗin amincin lantarki masu zuwa:

Alama

Bayani
Toshe robot ɗin cikin ƙasa mai da'ira, Class 1. Duba sashin Haɗin Wuta a cikin babin Siffanta Tsarin.

Kar a haɗa (toshe ciki), cire haɗin (cire haɗin), ko amfani da igiyoyin wutar AC idan: Kebul ɗin ya lalace ko ya lalace. Sauran igiyoyin da aka haɗe, igiyoyi, ko ma'ajin ajiya sun lalace ko sun lalace.
Yin amfani da igiyoyin wutar lantarki da suka lalace na iya haifar da haɗarin girgizar wutar lantarki wanda ke haifar da mummunan rauni ko lahani ga robot ɗin.
Kar a musanya kebul na wutar AC sai dai idan a jagorancin Opentrons Support.

Don ƙarin bayani game da buƙatun lantarki, duba sashin Amfani da Wutar Lantarki na babin Shigarwa da Matsarwa.

OPENTRONS FLEX

BABI NA 1: GABATARWA

Ƙarin gargaɗin aminci
Koyaushe kiyaye ƙarin gargaɗin aminci masu zuwa:

Alama

Bayani
Opentrons Flex ba a ba da takaddun shaida don amfani da abubuwa masu fashewa ko masu ƙonewa ba. Kar a ɗora faranti, bututu, ko filaye masu ɗauke da abubuwa masu fashewa ko masu ƙonewa a cikin mutum-mutumi ko in ba haka ba yi aiki da kayan aiki tare da abubuwa masu fashewa ko masu ƙonewa a cikin yadi.
Yi amfani da ayyuka masu kyau na dakin gwaje-gwaje kuma bi kariyar masana'anta lokacin aiki da sinadarai. Opentrons ba shi da alhakin ko alhakin kowane lalacewa saboda, ko sakamakon, amfani da sinadarai masu haɗari.

Flex yana auna kilo 88.5 (lbs 195). A sakamakon haka, yana buƙatar mutane biyu su ɗaga su kuma motsa shi cikin aminci. Dubi sashin Maɗaukaki a cikin Babin Shigarwa da Ƙaura.

Ya kamata a sanya Flex a saman da zai iya tallafawa nauyinsa na 88.5 kg (195 lbs) tare da isasshiyar wuri don ɗaukar mutum-mutumin tare da mafi ƙarancin nisa (20 cm/8 in). Dubi sashin Bukatun Tsaro da Aiki a cikin Babin Shigarwa da Matsarwa.
Flex na iya fitar da girgiza yayin aiki. Sanya mutum-mutumi a saman da yake da ƙarfi, matakin, kuma mai jure ruwa tare da igiyar igiyar igiya ko welded. Dubi sashin Bukatun Tsaro da Aiki a cikin Babin Shigarwa da Matsarwa.

Gargaɗi na aminci
Don taimakawa kare Flex daga lalacewa, bi waɗannan matakan tsaro:

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BABI NA 1: GABATARWA

Alama

Bayani
Yi amfani da labware wanda ya dace da ANSI/SLAS ko Opentrons ya amince da shi. Duba sashin Labware.
Ajiye abubuwa masu lalacewa, wakilai, ko wasu abubuwan da ke lalatawa nesa da mutum-mutumi.

Lafiyar halittu
Kula da samfurori da reagents masu ƙunshe da kayan da aka karɓa daga mutane azaman masu iya kamuwa da cuta. Opentrons yana ba da shawarar yin amfani da amintattun hanyoyin dakin gwaje-gwaje kamar yadda aka yi bayani a Biosafety a cikin Dakunan gwaje-gwajen Microbiological da Biomedical (BMBL) 6th Edition.
A ƙarƙashin yanayi na al'ada, Flex baya ƙirƙirar iska mai iya ganowa daga ruwa mai tushe. Koyaya, a ƙarƙashin wasu yanayi, yana yiwuwa a samar da iska daga ruwa mai tushe. Lokacin aiki tare da matakin biosafety matakin 2 ko mafi girma tushen ruwa mai tushe, yi la'akari da ɗaukar matakan kariya daga bayyanar da iska mai iska, daidai da hukumomin ku na gida. Don rage yuwuwar haɗarin faɗuwar iska daga robot, tabbatar da cewa:
Yi kulawa kamar yadda aka bayyana a cikin Babin Kulawa da Sabis. Shigar da kyau da kiyaye duk murfin kayan aiki, pipettes, kayayyaki, da labware. Yi amfani da dabarar bututun da ya dace don taimakawa wajen rage iska.
Turi mai guba
Idan kuna aiki tare da masu kaushi ko abubuwa masu guba, yi amfani da ingantaccen tsarin iska don cire duk wani tururi da za'a iya samarwa.
Ruwa masu ƙonewa
Ba a kimanta Flex don amfani da ruwa mai ƙonewa ba kuma bai kamata a yi amfani da shi da ruwa mai ƙonewa ba.

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BABI NA 1: GABATARWA

1.3 Yarda da tsari
Opentrons Flex ya bi duk buƙatun da suka dace na aminci da ka'idojin lantarki masu zuwa.

Tsaro
Dokar ID IEC/UL/CSA 61010-1 IEC/UL/CSA 61010-2-051

Take
Bukatun Tsaro don Kayan Aikin Lantarki don Aunawa, Sarrafa, da Amfani da Laboratory Sashe na 1: Gabaɗayan Bukatu
Abubuwan buƙatu na musamman don kayan aikin dakin gwaje-gwaje don haɗawa da motsawa

Daidaitawar lantarki

ID na doka EN/BSI 61326-1
FCC 47 CFR Kashi na 15 Karamin B Class A IC ICES-003

Take
Kayayyakin Wutar Lantarki don Aunawa, Sarrafa, da Amfani da Laboratory Buƙatun EMC Sashe na 1: Gabaɗaya Bukatun Radiators ba tare da niyya ba
Gudanarwar Spectrum da Tsangwama na SadarwaHaɗaɗɗen Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Fasaha na Watsa Labarai (ciki har da Na'urar Dijital)

FCC gargadi da bayanin kula
Gargaɗi: Canje-canje ko gyare-gyare ga wannan rukunin da Opentrons ba su yarda da shi ba na iya ɓata ikon mai amfani don sarrafa kayan aikin.
Wannan na'urar ta bi Sashe na 15 na dokokin FCC. Aiki yana ƙarƙashin abubuwa masu zuwa:

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BABI NA 1: GABATARWA
Wannan na'urar bazai haifar da tsangwama mai cutarwa ba. Dole ne wannan na'urar ta karɓi duk wani tsangwama da aka karɓa, gami da tsangwama wanda zai iya haifar da rashin so
aiki.
Lura: An gwada wannan kayan aikin kuma an same shi don biyan iyaka don na'urar dijital A Class A, bisa ga Sashe na 15 na dokokin FCC. An tsara waɗannan iyakokin don ba da kariya mai ma'ana daga tsangwama mai cutarwa lokacin da ake sarrafa kayan aiki a cikin yanayin kasuwanci. Wannan kayan aiki yana haifarwa, yana amfani da shi, kuma yana iya haskaka ƙarfin mitar rediyo kuma, idan ba'a shigar da shi ba kuma aka yi amfani dashi daidai da littafin koyarwa, na iya haifar da kutse mai cutarwa ga sadarwar rediyo. Yin aiki da wannan kayan aiki a cikin wurin zama yana iya haifar da tsangwama mai cutarwa wanda idan za a buƙaci mai amfani ya gyara tsangwama a cikin kuɗin nasu.
Kanada ISED yarda
Kanada ICES-003(A) / NMB-003(A)
Wannan samfurin ya haɗu da innovation, Kimiyya da Ci gaban Tattalin Arziki ƙayyadaddun fasaha na Kanada.
Le present produit est conforme aux fasahohin ƙayyadaddun bayanai sun dace da Innovation, Sciences et Developpement economique Kanada.
Gargadi na muhalli
Gargaɗi: Ciwon daji da Ciwon Haihuwa www.P65Warnings.ca.gov
Tabbatar da Wi-Fi
An inganta tsarin Wi-Fi don amfani a yankuna da yawa:
Amurka (FCC): Mai gano FCC UAY-W8997-M1216 Yankin Tattalin Arzikin Turai (CE): Babu mai gano jama'a (bayyana kansa) Kanada (IC): Lambar Shaidar Hardware W8997-M1216 Japan (TELEC): Lambar da aka tabbatar 020-170034 Indiya (WPC): Lambar rajista ETA-SD-20191005525 (bayanin kai)

OPENTRONS FLEX

BABI NA 2
Shigarwa da Matsarwa
Wannan babin yana bayyana yadda ake shirya lab ɗin ku don Opentrons Flex, yadda ake saita mutum-mutumi, da yadda ake motsa shi idan ya cancanta. Kafin ɗaukar isar da Flex ɗin ku, tabbatar da cewa ɗakin bincikenku ko kayan aikinku sun cika duk ƙa'idodi a cikin Safety da Buƙatun Aiki. Lokacin da lokaci ya yi don haɓaka Flex ɗin ku da aiki, bi cikakkun bayanai a cikin Unboxing, Run First, da Instrument Instrument and Calibration sections, ko amfani da sabis na Saitin Tallafi na Onsite Opentrons. Kuma idan kuna buƙatar matsar da Flex ɗin ku zuwa sabon wuri, kusa ko nesa, bi matakan da ke cikin sashin Matsar.
2.1 Tsaro da buƙatun aiki
Inda za a sanya Opentrons Flex
Sarari abu ne mai kima a kusan kowane lab. Flex ɗin ku zai buƙaci wasu-amma ba da yawa ba, saboda an ƙera shi don dacewa da rabin daidaitaccen benci na lab. Tabbatar cewa kana da sarari wanda ya cika ka'idoji masu zuwa.
Bench surface: Tsaye, sturdy, matakin, ruwa mai jure ruwa. Tebura ko benci masu ƙafafu (ko da makullin ƙafafun) ba a ba da shawarar ba. Flex yana motsawa da sauri kuma yana da tarin yawa, wanda zai iya girgiza ko rashin daidaiton nauyi ko teburi masu motsi.
Nauyin nauyi: Mutum-mutumi shi kadai yana da nauyin kilogiram 88.5 (195 lb) kuma mutane biyu ne kawai ke aiki tare. Sanya mutum-mutumi a saman wanda zai iya tallafawa nauyinsa da sauri tare da nauyin kowane nau'i, kayan aiki, ruwa, ko wasu kayan aikin lab da za a yi amfani da su a aikace-aikacenku.
Wurin aiki: Girman gindin robot ɗin shine 87 cm W x 69 cm D x 84 cm H (kimanin 34 ″ x 27″ x 33″). Flex yana buƙatar 20 cm (8 ") na gefe da baya don igiyoyi, haɗin USB, da kuma watsar da shaye-shaye daga na'urori masu zafi da sanyi.
Gargaɗi: Kada a sanya tarnaƙi ko baya na Flex a zubar da bango.

OPENTRONS FLEX

BABI NA 2: SHIGA DA MATSAYI

84 cm 33 ″

87 cm 34 ″
Opentrons Flex girman tushe.

69 cm 27 ″

20 cm 8 ″

Sama view na Opentrons Flex, yana nuna mafi ƙarancin gefe da baya.

OPENTRONS FLEX

BABI NA 2: SHIGA DA MATSAYI

Amfanin wutar lantarki
Opentrons Flex yakamata a haɗa shi zuwa mashin bango a ko kusa da wurin benci inda kuka shigar dashi. Haɗa Flex kawai zuwa da'irori waɗanda zasu iya ɗaukar iyakar ƙarfinsa:
Ƙarfin shigarwa: 36 VDC, 6.1 A Amfani mara amfani: 30 W Yawan amfani: 40 W (a yayin tafiyar da yarjejeniya) Matsakaicin amfani: Kimanin 50 W
Daidaitaccen amfani da wutar lantarki ya dogara da:
Adadi da nau'in motsi da aka aiwatar yayin yarjejeniya. Adadin lokacin da mutum-mutumin ke kashewa ba aiki. Matsayin fitilu akan robot. Nawa kayan aiki aka haɗa.
Ka tuna don lissafin wasu na'urorin lantarki waɗanda ke cinye wuta akan da'irar iri ɗaya, gami da na'urorin Flex tare da nasu wutar lantarki. Don misaliampHar ila yau, Module na Thermocycler yana da matsakaicin ƙarfin amfani (630 W) wanda ya fi na Flex robot kanta. Idan ya cancanta, tuntuɓi manajan kayan aikin ku don tabbatar da ya cika buƙatun wutar lantarki na kayan aikin ku.

Yanayin muhalli
Sharuɗɗan muhalli don amfani da shawarar da aka ba da shawarar, amfani mai karɓuwa, da ajiya sun bambanta:

An ba da shawarar don tsarin aiki

An yarda da tsarin aiki

Yanayin zafin jiki daga +20 zuwa +25 °C

+2 zuwa +40 ° C

Dangantakar zafi Matsayi

40%.
Kimanin mita 500 sama da matakin teku

30%, mara sanyaya (kasa da 80 °C)
Har zuwa 2000 m sama da matakin teku

Adana da sufuri
-10 zuwa +60 ° C
10%, mara sanyaya (kasa da 85 °C)
Har zuwa 2000 m sama da matakin teku

OPENTRONS FLEX

BABI NA 2: SHIGA DA MATSAYI
Opentrons ya inganta aikin Opentrons Flex a cikin yanayin da aka ba da shawarar don aiki na tsarin, kuma aiki a cikin waɗannan yanayi ya kamata ya samar da sakamako mafi kyau. Flex yana da aminci don amfani a cikin yanayin da aka yarda don aikin tsarin, amma sakamakon zai iya bambanta. Kar a kunna ko amfani da Flex a cikin yanayin waje na waɗannan iyakoki. Yanayin ajiya da sufuri yana aiki ne kawai lokacin da mutum-mutumi ya katse gaba ɗaya daga wuta da sauran kayan aiki.
2.2 Buɗewa
Taya murna! Opentrons Flex ɗinku ya isa kuma kun shirya masa sarari a cikin ɗakin binciken ku. Bari mu buɗe wannan rami na dodo, cire robot ɗin, mu shirya shi don aiki. Bayanin da ke cikin wannan sashe yana ba da jerin sassa da umarni waɗanda ke bi da ku ta matakan da ake buƙata don buɗe akwatin Flex, saita, kuma shirye don amfani. Mun raba tsarin saitin zuwa sassa uku:
Kashi na 1 ya ƙunshi tarwatsa akwatunan. Sashe na 2 ya ƙunshi cire Flex daga ramin da matsar da shi zuwa wurin taro na ƙarshe. Sashe na 3 ya ƙunshi taro na ƙarshe da ƙarfin aiki akan robot a karon farko.
Ƙoƙari da lokacin da ake buƙata
Kuna so ku tambayi abokin aikin lab don taimakawa tare da cirewa, ɗagawa, motsi, da tsarin haɗuwa. Kuna buƙatar tsara kasafin kusan mintuna 30 zuwa awa ɗaya don wannan ƙoƙarin.
Lura: Flex yana buƙatar mutane biyu don ɗaga shi daidai. Hakanan, ɗagawa da ɗaukar Flex ta hanunsa ita ce hanya mafi kyau don motsa mutum-mutumi.
Akwati da kayan tattarawa
Cire fakitin Flex yana ba ku mutum-mutumi mai ban sha'awa, amma kuma an bar ku da manyan fafutoci masu yawa tare da kayan jigilar kayayyaki iri-iri da padding. Yayin da za ku iya zubar da wannan kayan, muna ƙarfafa ku ku ajiye waɗannan abubuwan idan akwai sararin ajiya. Ana iya sake amfani da fakitin, wanda ke taimakawa shirya Flex ɗinku don jigilar kaya idan kuna buƙatar aika ta wani wuri dabam (misali, zuwa taro ko sabon wurin aiki) nan gaba.

OPENTRONS FLEX

BABI NA 2: SHIGA DA MATSAYI
Abubuwan samfur
Jirgin Flex tare da abubuwan da aka jera a ƙasa. Pipettes, gripper, da kayayyaki sun zo cikin marufi daban-daban daga babban akwati na Flex, ko da kun sayi su tare azaman wurin aiki.

(1) Opentrons Flex robot

(1) kebul na USB

(1) Kebul na wuta

(1) Ethernet na USB

(5) L-keys (12 mm hex, 1.5 mm hex, 2.5 mm hex, 3 mm hex,
T10 Torx)

(1) Tsayawar Gaggawa

(1) Ramin bene tare da shirye-shiryen labware

(4) Kayan kayan aikin labware

(1) Binciken daidaitawar Pipette

(4)Dauke hannaye da huluna

(1) Babban taga taga

(4) Bankunan taga

(1) 2.5mm hex sukurori

(1) 19mm madaukai

(16+ spares) Window sukurori (M4x8 mm lebur kai)
26

(10) Screws (M4x10 mm head socket)

(12) Screws (M3x6 mm shugaban soket)

OPENTRONS FLEX

BABI NA 2: SHIGA DA MATSAYI
Sashe na 1: Cire akwati
Opentrons yana jigilar Flex ɗin ku a cikin akwati mai ƙarfi. Akwatin jigilar kaya yana amfani da ƙugiya da latch clamps don tabbatar da saman, gefe, da na ƙasa tare. Yin amfani da latches, maimakon ƙusoshi ko screws, yana nufin ba za ku buƙaci maƙarƙashiya (ko ƙarfi mai yawa) don kwance akwati ba, kuma za ku iya sake haɗa shi daga baya, idan an buƙata.
Lura: Gefen ramuka na iya yin muni yayin jigilar kaya. Kuna iya amfani da safar hannu na aiki don kare hannayenku daga tsagewar itace.
Don sakin latches, juye shafin latch sama kuma juya shi zuwa hagu (madaidaicin agogo). Wannan aikin yana motsa clamp hannu daga madaidaicin sashi mai riƙewa. Sannan zaku iya juyar da hannun latch ɗin daga ramin.
1 Buɗe latches takwas ɗin da ke riƙe saman zuwa tarnaƙi.

2 Cire babban kwamiti bayan sakin latches.

OPENTRONS FLEX

BABI NA 2: SHIGA DA MATSAYI 3 Yanke buhunan shudin na jigilar kaya, cire wadannan abubuwan daga cikin madodin, sannan a ajiye su a gefe:
Ƙarfin Kit ɗin mai amfani, Ethernet, da igiyoyin USB na Tsayar da Gaggawa Tsayawa
4 Cire saman saman kumfa don fallasa faifan taga. Padding yana kare gefen da manyan bangarori.
5 Cire bangarorin taga kuma ajiye su a gefe. Za ku haɗa waɗannan daga baya.

OPENTRONS FLEX

BABI NA 2: SHIGA DA MATSAYI
6 Buɗe sauran latches 16 waɗanda ke riƙe da sassan gefe da juna da gindin ramin. 7 Cire sassan gefen kuma ajiye su a gefe.
Sashe na 2: Saki Flex
Bayan kammala matakan da ke cikin Sashe na 1, yanzu ya kamata ka ga wani mutum-mutumi wanda ke cikin jakar kariya kuma an makala shi da kayan hawan ƙarfe na orange. Jakar tana rufe robobin kuma tana kare shi daga yanayin waje. Maƙarƙashiyar ƙarfe ta amintar da mutum-mutumin zuwa kasan ramin. Firam ɗin jigilar kaya guda biyu suna goyan bayan robobin, suna rarraba nauyinsa daidai gwargwado, da kuma kiyaye shi da tsayin daka don kada ya karkata yayin jigilar kaya. Ci gaba da cire kayan Flex kuma cire shi daga gindin akwati.
8 Yin amfani da maƙarƙashiya mm 19 daga Kit ɗin Mai amfani, buɗe maƙallan daga ƙasan rami. Kuna iya jefar da maƙallan, ko ajiye su don amfani na gaba.

OPENTRONS FLEX

BABI NA 2: SHIGA DA MATSAYI 9 Ja ko mirgine jakar jigilar kaya zuwa ƙasa don fallasa duk robot ɗin.
10 Tare da taimako daga abokin aikin lab ɗin ku, ɗauki hannaye a cikin firam ɗin jigilar kaya orange a kowane gefen gindin robot ɗin, ɗaga Flex daga gindin akwati, sannan saita shi a ƙasa. Ajiye ko jefar da gindin akwati da firam ɗin jigilar kaya.

OPENTRONS FLEX

BABI NA 2: SHIGA DA MATSAYI 11 Yin amfani da maɓalli na hex L-mm 12 daga Kit ɗin Mai amfani, cire kusoshi huɗu masu riƙe da firam ɗin jigilar kaya zuwa
Flex. Ajiye ko jefar da firam ɗin da kusoshi.
12 Cire hanun aluminium huɗu daga Kit ɗin Mai amfani. Matsar da hannaye a cikin wurare iri ɗaya waɗanda ke riƙe da kusoshi na jigilar mm 12.

OPENTRONS FLEX

BABI NA 2: SHIGA DA MATSAYI
13 Tare da taimako daga abokin aikin lab ɗin ku, ɗaga Flex ta hannun ɗaukarsa kuma matsar da shi zuwa wurin aiki don taro na ƙarshe.

Sashe na 3: Haɗuwa ta ƙarshe da kunnawa
Bayan matsar da Flex zuwa wurin aiki na wucin gadi, ko gidansa na dindindin, lokaci yayi da za a sanya abubuwan gamawa akan sabon robot ɗin ku.
14 Idan kun matsar da mutum-mutumin zuwa wurinsa na ƙarshe, inda yake aiki, cire hannayen ɗauka kuma ku maye gurbinsu da iyakoki na gamawa. Maƙallan suna rufe buɗewar hannu a cikin firam ɗin kuma suna ba wa mutum-mutumin kamanni mai tsabta. Mayar da hannaye zuwa Kit ɗin Mai amfani don ajiya.

OPENTRONS FLEX

BABI NA 2: SHIGA DA MATSAYI
15 Maido saman da gefen gefen daga kumfa ɗin da kuka ajiye a gefe bayan cire saman kwalin.
16 Daidaita ginshiƙan taga zuwa Flex ta bin bayanin alamar akan fim ɗin kariya na gaba. Sa'an nan kuma cire fim ɗin kariya.
17 Yin amfani da kusoshi na taga da screwdriver mm 2.5 daga Kit ɗin Mai amfani, haɗa fakitin taga zuwa Flex. Tabbatar cewa ramukan beveled (V-dimbin yawa) a cikin bangarorin taga suna fuskantar waje (zuwa gare ku). Wannan yana ba da damar skru su dace da saman taga.

Gargaɗi: Ba daidai ba daidaita bangarorin na iya haifar da lalacewa. Matsananciyar jujjuyawar juzu'i na iya fashe bangarorin. Hannu danne sukurori har sai ginshiƙan taga sun sami amintaccen tsaro. Wannan ba gwaji ne na ƙarfi ba.

OPENTRONS FLEX

BABI NA 2: SHIGA DA MATSAYI 18 Yin amfani da screwdriver 2.5 mm daga Kit ɗin Mai amfani, cire skru na kulle daga gantry. Wadannan
screws suna hana gantry motsi yayin da suke wucewa. Wuraren kulle gantry suna nan: A kan titin gefen hagu kusa da gaban robot. Ƙarƙashin hannun gantry na tsaye. A gefen dogo na dama kusa da gaban mutummutumi a cikin madaidaicin lemu. Akwai sukurori biyu a nan.
Gantry yana motsawa cikin sauƙi da hannu bayan cire duk skru na jigilar kaya. 19 Yanke kuma cire igiyoyin roba guda biyu waɗanda suke riƙe kwandon shara a wurin yayin jigilar kaya.

OPENTRONS FLEX

BABI NA 2: SHIGA DA MATSAYI
20 Haɗa igiyar wutar lantarki zuwa Flex kuma toshe ta cikin mashin bango. Tabbatar cewa wurin ba shi da cikas. Juya wutar lantarki a gefen hagu na robot ɗin. Da zarar an kunna, gantry yana motsawa zuwa wurin gida kuma allon taɓawa yana nuna ƙarin umarnin daidaitawa.

Yanzu da Flex ɗin ku ya fita daga cikin akwatin kuma yana shirye don tafiya, ci gaba zuwa sashin Run na Farko a ƙasa.
2.3 Gudun farko
Yi saitin asali akan allon taɓawa kafin haɗa duk wani kayan aiki zuwa Flex ɗin ku. Robot ɗin zai jagorance ku ta hanyar haɗa zuwa cibiyar sadarwar ku, sabunta zuwa sabuwar software, da keɓance Flex ta hanyar ba ta suna.
A kunne
Lokacin da kuka kunna Flex, tambarin Opentrons zai bayyana akan allon taɓawa. Bayan 'yan mintuna kaɗan, zai nuna allon "Barka da zuwa ga Opentrons Flex ɗin ku".
Allon maraba da Opentrons Flex. Ya kamata ku ga wannan allon kawai lokacin da kuka fara Flex ɗin ku a karon farko.

OPENTRONS FLEX

BABI NA 2: SHIGA DA MATSAYI
Haɗa zuwa cibiyar sadarwa ko kwamfuta
Bi abubuwan faɗakarwa akan allon taɓawa don haɗa mutum-mutumin ku ta yadda zai iya bincika sabunta software da karɓar yarjejeniya files. Akwai hanyoyin haɗin kai guda uku: Wi-Fi, Ethernet, da USB.

Zaɓuɓɓukan haɗin yanar gizo. Kuna buƙatar samun haɗin intanet don saita Flex. Wi-Fi: Yi amfani da allon taɓawa don haɗawa da cibiyoyin sadarwar Wi-Fi waɗanda aka amintattu tare da ingantaccen WPA2 (mafi yawan cibiyoyin sadarwar da ke buƙatar kalmar sirri kawai don shiga suna faɗi ƙarƙashin wannan rukunin).
Lura: Flex baya goyan bayan mashigai na kama (cibiyoyin da ba su da kalmar sirri amma suna loda a webshafi don tabbatar da masu amfani bayan haɗawa).
Hakanan zaka iya haɗawa zuwa cibiyar sadarwar Wi-Fi buɗe, amma wannan ba a ba da shawarar ba.
Gargaɗi: Haɗa zuwa cibiyar sadarwar Wi-Fi da ke buɗe zai ba duk wanda ke cikin kewayon siginar hanyar sadarwar damar sarrafa robot ɗin Opentrons Flex ɗin ku ba tare da tantancewa ba.

Idan kana buƙatar haɗawa zuwa cibiyar sadarwar Wi-Fi da ke amfani da amincin kamfani (ciki har da "eduroam" da makamantan cibiyoyin sadarwar ilimi waɗanda ke buƙatar sunan mai amfani da kalmar wucewa), fara haɗawa zuwa Opentrons App ta Ethernet ko USB don kammala saitin farko. Sannan haɗa zuwa cibiyar sadarwar Wi-Fi na kasuwanci a cikin saitunan sadarwar don Flex ɗin ku. Don samun dama ga saitunan sadarwar:

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BABI NA 2: SHIGA DA MATSAYI
1. Danna Devices a gefen hagu na Opentrons App. 2. Danna menu mai digo uku () don Flex ɗin ku kuma zaɓi Saitin Robot. 3. Danna Networking shafin.
Zaɓi cibiyar sadarwar ku daga menu na zazzage ko zaɓi "Haɗa sauran hanyar sadarwar..." kuma shigar da SSID. Zaɓi hanyar tabbatar da kasuwancin da cibiyar sadarwar ku ke amfani da ita. Hanyoyin tallafi sune:
EAP-TTLS tare da TLS EAP-TTLS tare da MS-CHAP v2 EAP-TTLS tare da MD5 EAP-PEAP tare da MS-CHAP v2 EAP-TLS
Kowace waɗannan hanyoyin suna buƙatar sunan mai amfani da kalmar wucewa, kuma dangane da ainihin tsarin sadarwar ku na iya buƙatar takaddun shaida files ko wasu zažužžukan. Tuntuɓi takaddun IT na kayan aikin ku ko tuntuɓi manajan IT don cikakkun bayanai na saitin cibiyar sadarwar ku.
Ethernet: Haɗa robot ɗin ku zuwa maɓalli na cibiyar sadarwa ko cibiya tare da kebul na Ethernet. Hakanan zaka iya haɗa kai tsaye zuwa tashar Ethernet akan kwamfutarka, farawa daga tsarin tsarin robot 7.1.0.
USB: Haɗa kebul na USB A-zuwa-B da aka tanadar zuwa tashar USB-B ta robot da buɗaɗɗen tashar jiragen ruwa akan kwamfutarka. Yi amfani da kebul na USB B-to-C ko adaftar USB A-zuwa C idan kwamfutarka ba ta da tashar USB-A.
Don ci gaba da saitin, kwamfutar da aka haɗa dole ne a shigar da Opentrons App kuma tana aiki. Don cikakkun bayanai kan shigar da Opentrons App, duba sashin shigar da App na babin Software da Aiki.
Shigar da sabunta software
Yanzu da kun haɗa zuwa cibiyar sadarwa ko kwamfuta, robot na iya bincika sabunta software da firmware kuma zazzage su idan an buƙata. Idan akwai sabuntawa, yana iya ɗaukar ƴan mintuna don shigarwa. Da zarar sabuntawar ya cika, robot ɗin zai sake farawa.

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BABI NA 2: SHIGA DA MATSAYI
Haɗa Tsayar da Gaggawa Pendant
Haɗa abin da aka haɗa na Tsaida Gaggawa (E-stop) zuwa tashar taimako (AUX-1 ko AUX-2) a bayan robot.

Kafin da bayan haɗa Alamar Tsaida Gaggawa.
Haɗawa da kunna tashar E-stop ya zama tilas don haɗa kayan aiki da ƙa'idodi masu gudana akan Flex. Don ƙarin bayani kan amfani da E-Stop yayin aikin mutum-mutumi, duba sashin Tsaida Tsaida Gaggawa na babin Siffanta Tsarin.
Ka ba mutum-mutumin suna
Sanya sunan mutum-mutumi naka yana ba ka damar gane shi cikin sauƙi a cikin mahallin lab ɗin ku. Idan kuna da mutum-mutumi na Opentrons da yawa akan hanyar sadarwar ku, tabbatar da ba su sunaye na musamman. Da zarar ka tabbatar da sunan mutum-mutumi naka, za a kai ka zuwa Opentrons Flex Dashboard. Wataƙila mataki na gaba da za ku so ɗauka shine haɗa kayan aiki, waɗanda aka rufe a sashe na gaba.
2.4 Shigar kayan aiki da daidaitawa
Bayan saitin mutum-mutumi na farko, mataki na gaba shine haɗa kayan aiki zuwa mutum-mutumi da daidaita su.
Don shigar da kayan aiki, da farko danna Instruments akan allon taɓawa ko je zuwa sashin Pipettes da Modules na allon dalla-dalla na na'urar a cikin Opentrons App. Zaɓi dutsen da babu komai kuma zaɓi ko dai Haɗa Pipette ko Haɗa Gripper. Idan dutsen da kuke son amfani da shi ya riga ya mamaye, kuna buƙatar cire pipette ko gripper da farko.

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BABI NA 2: SHIGA DA MATSAYI
Lura: Tsarin shigarwa gabaɗaya iri ɗaya ne ko da kuna amfani da allon taɓawa ko Opentrons App. Duk na'urar da kuka fara za ta sarrafa tsarin shigarwa har sai kun gama ko soke ta.
Idan ka fara kan allon taɓawa, ƙa'idar za ta nuna mutum-mutumin a matsayin "aiki". Idan ka fara a cikin ƙa'idar, allon taɓawa zai nuna yanayin da ke nuna cewa ana ci gaba da shigar da kayan aikin.
Madaidaicin tsarin shigarwa ya bambanta dangane da kayan aikin da kuke haɗawa, kamar yadda aka rufe a cikin sassan da ke ƙasa. Duk kayan aikin suna da tsarin daidaitawa mai sarrafa kansa, wanda yakamata ku yi nan da nan bayan shigarwa.
Shigar da pipette
Lokacin da kuka shigar da pipette, za a jagorance ku ta matakai masu zuwa akan allon taɓawa ko a cikin Opentrons App.
1. ZABI NAU'IN PIPETTE Zaɓi tsakanin 1- ko 8-Channel Pipette da 96-Channel Pipette. Haɗa Pipette na 96-Channel yana buƙatar ƙarin matakai kaɗan saboda yana haɗawa da faranti na musamman wanda ya mamaye duka pipette.
2. SHIRI DON SHIRYA Cire labware daga bene kuma tsaftace wurin aiki don sauƙaƙe haɗe-haɗe da daidaitawa. Har ila yau tattara kayan aikin da ake buƙata, kamar na'urar tantancewa, screwdriver hex, da farantin hawa (don 96-Channel Pipette).
3. HAƊA DA TSARE PIPETTE Gantry zai matsa zuwa gaban robot don haka za ku iya haɗa pipette.
1- da 8-Channel Pipettes suna haɗa kai tsaye zuwa dutsen pipette. Pipette 96-Channel yana buƙatar farantin hawa. Domin haɗa farantin hawa, dole ne ka fara cire haɗin jigilar z-axis don madaidaicin pipette.

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BABI NA 2: SHIGA DA MATSAYI
Haɗa pipette zuwa dutsen pipette da aka zaɓa kuma aminta da sukurori.
4. GUDANAR DA CALIBRATION AUTOMAN Don daidaita pipette, haɗa bincike na daidaitawa zuwa bututun pipette mai dacewa. Pipette zai matsa ta atomatik don taɓa wasu maki akan bene kuma ya adana waɗannan ƙimar ƙima don amfani na gaba. Da zarar an gama daidaitawa kuma kun cire binciken, pipette zai kasance a shirye don amfani a cikin ladabi.
Shigarwa Gripper
Lokacin da kuka shigar da gripper, za a jagorance ku ta hanyar matakai masu zuwa akan allon taɓawa ko a cikin Opentrons App.
1. SHIRI DON SHIRYA Cire labware daga bene kuma tsaftace wurin aiki don sauƙaƙe haɗewa da daidaitawa. Hakanan tattara hex screwdriver da ake buƙata kuma a tabbata cewa fil ɗin daidaitawa yana cikin wurin ajiyarsa akan abin riko.
2. HAƊA DA TSARE MAI GIRMAN Gantry ɗin zai matsa zuwa gaban mutum-mutumi don haka za ku iya haɗa abin riko. Haɗa gripper zuwa dutsen tsawaita kuma kiyaye sukurorin sa.
3. GUDU CALIBRATION AUTOMAN Don daidaita abin riko, saka fil ɗin daidaitawa a muƙamuƙi na gaba. Mai riko zai matsa ta atomatik don taɓa wasu maki akan bene kuma ya adana waɗannan ƙimar ƙimar don amfani na gaba. Sa'an nan kuma maimaita wannan tsari tare da fil ɗin daidaitawa a cikin muƙamuƙi na baya. Da zarar an gama daidaitawa kuma kun mayar da fil ɗin a wurin ajiyarsa, mai riko zai kasance a shirye don amfani a cikin ladabi.
2.5 Matsar
Wannan sashe yana ba da shawara da umarni game da yadda ake matsar da mutum-mutumi na Opentrons Flex kan gajere da dogon nesa.

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BABI NA 2: SHIGA DA MATSAYI
Gajeren motsi
Wani ɗan gajeren tafiya yana ɗaukar nisa da yawa daga “bari mu matsar da shi kaɗan kaɗan” zuwa ƙetaren lab, ƙasa zauren, ko wani bene a cikin ginin ku. A wannan yanayin, zaku iya motsa Flex ɗin ku da hannu. Yin jigilar shi a kan keken hannu shima zaɓi ne mai kyau.
Gargaɗi: Flex yana auna kilo 88.5. A sakamakon haka, yana buƙatar mutane biyu su ɗaga su kuma motsa shi cikin aminci.
Sake haɗa hannayen ɗagawa don matsar da Flex ɗin ku zuwa sabon wuri, kusa. Ɗagawa da ɗaukar Flex ta hannayensa ita ce hanya madaidaiciya don matsar da mutum-mutumin gajeriyar nisa. Cire hannaye kuma adana su a cikin Kit ɗin Mai amfani bayan an gama motsi. Don hana lalata mutum-mutumi, koyaushe yi amfani da hannayen ɗagawa don ɗauka da motsa shi. Kar a ɗauki firam ɗin don ɗagawa ko motsa mutum-mutumi naka.
Motsa jiki mai nisa
Motsi mai nisa yana jigilar Flex ɗin ku daga filaye na jami'ar ku, wurin aiki, ko cibiyar ku. A ko'ina cikin gari, zuwa sabon birni, jiha, lardi, ko ƙasa duk examples na tafiya mai nisa. A wannan yanayin, kuna buƙatar shirya Flex don kare shi daga abubuwa, firgita, da matsananciyar motsi waɗanda ka iya faruwa yayin tafiya.
Idan kun adana akwatun jigilar kaya da tallafi na ciki waɗanda suka zo tare da Flex ɗinku, zaku iya sake tattara shi cikin waɗannan kayan don tafiya mai nisa. Bi matakan kwancewa a baya domin shirya Flex ɗinku don tafiya mai nisa. Ainihin, ya kamata ku:
Cire haɗin wutar lantarki da kebul na cibiyar sadarwa, idan haɗe. Cire duk kayan aikin da aka haɗe da labware. Sake haɗa faranti na bene. Kulle gantry (duba Babban Shawarar Motsawa a ƙasa). Cire kuma adana bangarorin taga.
Idan kun ajiye akwati na asali:
Sake haɗa firam ɗin jigilar kaya zuwa Flex kuma aminta da shi zuwa gindin pallet ta amfani da madaidaicin L. Ƙara manne da sake haɗa akwatunan jigilar kaya.

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BABI NA 2: SHIGA DA MATSAYI
Idan ba ku da ainihin akwati da kayan da ke da alaƙa, tuntuɓi wani kamfani na jigilar kaya mai suna. Za su iya sarrafa jigilar kaya, sufuri, da tsarin isarwa gare ku.
Gabaɗaya nasiha mai motsi
CUTAR DA WUTA DA CIWON NETWORK Kafin motsa Flex ɗinka, kar a manta: Kashe wutar lantarki kuma cire shi daga wutar lantarki. Cire haɗin Ethernet ko kebul na USB, idan ana amfani da su.
KULLE GANTRY Kafin matsar da Flex ɗin ku, sake saka skru na kulle don riƙe gantry a wurin. Wuraren kulle gantry suna nan: A kan titin gefen hagu kusa da gaban robot. Ƙarƙashin hannun gantry na tsaye. A gefen gefen dama kusa da gaban robot. Kulle wannan ɓangaren gantry yana buƙatar ƙananan
Bakin orange da kusoshi na kulle biyu.

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BABI NA 2: SHIGA DA MATSAYI
GIDA GANTRY Wataƙila ba za ku so ku kulle gantry ba idan kuna matsar da mutum-mutumi zuwa wani wuri kusa. Idan kun yanke shawarar kada ku kulle shi, aƙalla yi amfani da allon taɓawa ko Opentrons App don aika gantry zuwa matsayinsa na gida kafin kunna shi. Don gida gantry ta fuskar taɓawa, matsa menu mai dige uku () sannan ka matsa Gidan gantry. Don gida gantry ta Opentrons App: Danna na'urori. Danna kan Flex ɗin ku a cikin jerin na'urar. Danna menu mai digo uku () sannan ka danna Home gantry.
Cire Modules In-deck modules da sauran haɗe-haɗe suna ƙara ƙarin nauyi ga Flex ɗin ku. Hakanan suna shafar cibiyar motsin mutum-mutumi, wanda zai iya sa ya ji “karkayi” lokacin ɗaga shi. Don taimakawa sauƙaƙa da daidaita mutum-mutumi, cire duk wani kayan aikin da aka makala da labware kafin ɗauka.
SAKE SHIGA DECK SLOTS Muna bada shawarar sake haɗa ramukan bene don tafiya mai nisa. Tabbatar da ramummuka a wurarensu na asali yana taimakawa hana asarar bazata. Sake haɗa ramukan bene don gajerun motsi a kusa da lab ɗin zaɓi ne.
GYARAN MATSUWA BAYANI Ya kamata ku sake daidaita kowane kayan kida da kayayyaki bayan sake shigar da su. Don ƙarin cikakkun bayanai kan daidaita tsarin, duba babin Modules.

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BABI NA 2: SHIGA DA MATSAYI
Tunani na ƙarshe game da motsi
Flex ɗin ku na'ura ce mai ƙarfi kuma ingantaccen gini, amma kuma takamaiman kayan aikin kimiyya ne wanda aka ƙera don ɗaukar haƙuri. A sakamakon haka, ya kamata ku bi da shi da kulawa yayin ƙaura zuwa yankin aikin ku na gida ko aika shi a cikin ƙasa. Wannan yana nufin bin jagorar da aka bayar anan da yin amfani da hankalin ku game da yadda ake jigilar kayan aikin dakin gwaje-gwaje masu tsada. Layin ƙasa: lokacin motsa Flex ɗin ku, yi kuskure a gefen taka tsantsan da ƙarin fakiti.
Idan kuna da tambayoyi ko damuwa game da ƙaura Flex ɗinku, tuntuɓe mu a support@opentrons.com.

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BABI NA 3
Bayanin Tsarin

Wannan babin yana bayyana tsarin kayan masarufi na Opentrons Flex, wanda ke ginshiƙan ainihin fasalulluka na kayan aikin lab. Dutsen bene, gantry, da kayan hawan kayan aiki na Opentrons Flex suna ba da damar yin amfani da madaidaicin ruwa da abubuwan sarrafa labware. Allon taɓawa na kan na'urar yana ba da damar gudanar da ka'idoji da duba matsayin mutum-mutumi ba tare da buƙatar kawo kwamfutarka zuwa benci na lab ba. Haɗin waya da mara waya yana ba da damar ƙarin iko daga Opentrons App (duba babin Software da Aiki don ƙarin cikakkun bayanai) da faɗaɗa fasalin tsarin ta haɗe-haɗe (duba babin Modules).

3.1 Abubuwan da ke cikin jiki

Kamara

Hasken Matsayi

Kariyar tabawa

Frame

Gantry Deck
Ƙofar Gaba
Wuraren abubuwan haɗin jiki na Opentrons Flex.

Side Windows Handle Caps

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BABI NA 3: BAYANIN TSARI

Frame da shinge
Firam ɗin Robot na Opentrons Flex yana ba da tsattsauran ra'ayi da tallafi na tsari don bene da gantry. Duk na'urorin injina suna kan su kuma an ɗora su zuwa babban firam. An gina firam ɗin ne da farko da ƙarfe na takarda da kuma extrusions na aluminum.
Firam ɗin ƙarfe yana da buɗewa don tagogin gefe da ƙofar gaba da aka yi da polycarbonate na zahiri wanda zai ba ku damar ganin abin da ke faruwa a cikin Flex. Ƙofar ƙofar gaba tana buɗewa don samun damar shiga cikin tsarin. Tare da buɗe ƙofar gaba, za ku iya haɗa kayan aiki, kayayyaki, da kayan aiki na bene; shirya bene kafin yarjejeniya; ko sarrafa yanayin bene yayin yarjejeniya.
Farar firam ɗin LED a saman saman gefuna na firam ɗin suna ba da hasken yanayi mai sarrafa software. Kyamara 2-megapixel na iya ɗaukar bene da wurin aiki don yin rikodi da bin diddigin aiwatar da ƙa'idar.

Deck da wurin aiki
Tushen shine saman aluminum da aka kera wanda akan aiwatar da ka'idojin kimiyyar sarrafa kansa. Gidan bene yana da manyan ramummuka guda 12 na ANSI/SLAS waɗanda za'a iya sake saita su don riƙe labware, kayayyaki, da abubuwan amfani. Ana gano ramukan bene ta tsarin daidaitawa, tare da Ramin A1 a hagu na baya da Ramin D3 a gaban dama.

Ramin Faɗawa (na Thermocycler) Wurin Aiki

Stagshiga Yanki

Yankunan bene a cikin Flex.

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BABI NA 3: BAYANIN TSARI
Wurin aiki shine sararin samaniya a sama da bene wanda ke da damar yin amfani da bututu. Labware da aka sanya a cikin ramummuka A1 zuwa D3 suna cikin wurin aiki.
Opentrons Flex ya zo tare da ramummuka masu cirewa don duk matsayi 12 a cikin wurin aiki. Kowane ramin bene yana da shirye-shiryen bidiyo na kusurwa don sanya labware a kan bene.
Kuna iya sake saita bene ta hanyar maye gurbin ramummuka tare da sauran kayan gyara bene, gami da sharar da ake iya motsi, sharar sharar gida, da kadi na module. Ramin fadada bayan A1 ana amfani dashi kawai don yin ƙarin ɗaki don Module na Thermocycler, wanda ya mamaye ramummuka A1 da B1.
Lura: Ramin bene suna musanya tsakanin ginshiƙi (1, 2, ko 3) amma ba a cikin ginshiƙai ba; shafi na 1 da shafi na 3 ramummuka ne daban-daban duk da girmansu iri ɗaya. Kuna iya sanin ko wane ginshiƙi ramin ke shiga ta hanyar daidaita hoton labware shuɗi zuwa hagu na baya.
Ya kamata ku bar ramukan bene da aka sanya a wuraren da kuke son sanya labware na tsaye. Wurin jirgin da abubuwan da aka ɗora akan sa sun kasance a tsaye, sai dai idan mai riko ko sa hannun hannu ya motsa.
Stagyanki
A stagyankin ing shine ƙarin sarari tare da gefen dama na bene. Kuna iya adana labware a wannan wurin bayan shigar da staging area ramummuka. Labware da aka sanya a cikin ramummuka A4 zuwa D4 suna cikin stagyankin. Flex pipettes ba zai iya shiga cikin staging area, amma gripper na iya ɗauka da motsa labware zuwa kuma daga wannan wurin. Ƙara ƙarin ramummuka yana taimakawa samar da wurin aiki don kayan aikin da ake amfani da su a cikin ƙa'idodin ku na atomatik.
Staging yankin ramummuka an haɗa su a cikin wasu saitunan wurin aiki kuma ana samun su don siye daga https://shop.opentrons.com.

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BABI NA 3: BAYANIN TSARI

StagWurin da aka Sanya Ramin

Kayan aiki na bene
Fixtures abubuwa ne na kayan masarufi waɗanda ke maye gurbin daidaitattun ramummuka na bene. Suna ba ku damar tsara shimfidar bene da ƙara ayyuka zuwa Flex ɗin ku. A halin yanzu, kayan aikin bene sun haɗa da stagWuraren yanki, kwandon shara na ciki, da sharar gida na waje. Kuna iya shigar da kayan aiki kawai a cikin ƴan takamaiman ramummuka na bene. Teburin da ke gaba yana lissafin wuraren bene na kowane kayan aiki.

Fixture Staging area ramummuka shara shara sharar sharar sharar sharar sharar gida tare da staging area slot

Ramin A3D3 A1D1 da A3-D3 D3 kawai D3 kawai

Kayan aiki ba su da ƙarfi. Ba su ƙunshi kayan lantarki ko injiniyoyi waɗanda ke sadar da yanayinsu na yanzu da wurin bene ga robot ɗin ba. Wannan yana nufin dole ne ku yi amfani da fasalin daidaitawar bene don sanar da Flex abubuwan da aka haɗa da bene da kuma inda suke.

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BABI NA 3: BAYANIN TSARI

Kuna iya samun dama ga saitunan daidaitawar bene daga allon taɓawa ta hanyar menu mai dige uku () da kuma daga App ɗin Opentrons. Dubi sashin Kanfigareshan Deck na babin Software da Aiki don bayani kan yadda ake saita bene daga allon taɓawa.

Sharar gida
Opentrons Flex Waste Chute yana canja wurin ruwa, tukwici, tukwici, da faranti daga wurin Flex zuwa ma'aunin shara da aka sanya a ƙasan buɗewar waje. Wurin shara yana manne da adaftar farantin bene wanda yayi daidai da Ramin D3. Hakanan yana zuwa tare da taga na musamman na rabin panel wanda ke ba da damar chute ɗin ya miƙe daga gaban robot ɗin.
Abubuwan da ke cikin sharar gida.

Adaftar Wuta Mai Rufe

Sharar gida
Deck Plate Adapter tare da Stagshiga Yanki

Staging area ramummuka
Staging area ramummuka su ne ANSI/SLAS guda bene masu jituwa waɗanda ke maye gurbin daidaitattun ramummuka a shafi na 3 kuma suna ƙara sabbin ramummuka zuwa s.tagyanki - duk ba tare da rasa sarari a wurin aiki ba. Kuna iya shigar da ramin guda ɗaya ko matsakaicin ramuka huɗu don ƙirƙirar sabon shafi (A4 zuwa D4) tare da gefen dama na bene. Lura, duk da haka, cewa maye gurbin bene A3 yana buƙatar motsa kwandon shara. Ta hanyar ƙara stagTare da ramukan yanki zuwa bene, robot ɗin ku na Flex na iya adana ƙarin labware kuma yayi aiki da inganci.

OPENTRONS FLEX

Flex staging area slot.
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BABI NA 3: BAYANIN TSARI
SLOT SHIGA
Don shigarwa, cire sukurori waɗanda ke haɗa daidaitaccen ramin zuwa bene kuma musanya shi da staging area slot. Bayan shigarwa, yi amfani da allon taɓawa ko Opentrons App don gaya wa robot ɗin da kuka ƙara a matsayintaging area Ramin zuwa bene.
Ana shigarwa azamantaging area slot.

RABUWA DA RABO StagRamin yanki sun dace da kayan aikin Flex, kayayyaki, da labware da aka jera a ƙasa.

Abubuwan Flex Gripper Pipettes Modules
Labware

Stagdaidaita yankin
Flex Gripper na iya motsa labware zuwa ko daga staging area ramummuka.
Flex pipettes ba zai iya isa ga stagyankin. Yi amfani da gripper don matsar da tukwici da labware daga staging yankin zuwa wurin aiki kafin bututu.
Ana iya sanya Magnetic Block GEN1 a shafi na 3 a saman astaging area slot. Ba a samun goyan bayan moduloli a shafi na 4.
Abubuwan da aka yi amfani da su kamar Heater-Shaker da Module Temperature sun dace cikin caddies waɗanda za a iya sanya su a shafi na 3. Ba za ku iya ƙara kamar yadda kuke so ba.tagRamin yanki zuwa matsayi wanda keɓaɓɓen module caddy.
StagRamin yanki suna da girman ANSI/SLAS iri ɗaya kamar daidaitattun ramummuka. Yi amfani da labware masu dacewa da gripper a cikin stagyankin, ko ƙara da cire kayan aikin labware daga wannan wurin.

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BABI NA 3: BAYANIN TSARI
Tsarin motsi
A haɗe da firam ɗin akwai gantry, wanda shine tsarin motsi da tsarin sakawa na robot. Gantry yana motsawa daban tare da x- da y-axis don sanya pipettes da gripper a daidaitattun wurare don aiwatar da yarjejeniya. Motsi tare da waɗannan gatura daidai yake zuwa 0.1 mm mafi kusa. Ana sarrafa gantry ta 36 VDC matasan bipolar stepper motors. Bi da bi, a haɗe zuwa gantry ne pipette firam da tsawo tsawo. Waɗannan suna motsawa tare da axis z don sanya pipettes da gripper a daidaitattun wurare don aiwatar da yarjejeniya. Motsi tare da wannan axis ana sarrafa ta 36 VDC matasan bipolar stepper Motors. Na'urorin lantarki da ke cikin gantry suna ba da wutar lantarki 36 VDC da sadarwa zuwa pipettes da gripper, lokacin da aka haɗa su.
Gantry

Pipette Dutsen

Extension Dutsen

Wurin hawa kayan aiki akan Flex.

Touchscreen da LED nuni
Babban abin da ake amfani da shi shine allon taɓawa na inch 7 LCD, wanda yake a gaban dama na mutum-mutumi. An rufe allon taɓawa da Gorilla Glass 3 don juriya da lalacewa. Samun dama ga abubuwa da yawa na Flex dama akan allon taɓawa, gami da:

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BABI NA 3: BAYANIN TSARI

Saitin tsarin yarjejeniya, aiwatarwa, da sa ido kan sarrafa Labware Saitunan Robot Software na tsarin da sabunta firmware Rajistar ayyuka da sanarwar kuskure
Don ƙarin bayani kan amfani da Flex ta fuskar taɓawa, duba sashin Aiki na Touchscreen na babin Software da Aiki.
Hasken matsayi wani tsiri ne na LEDs tare da saman gaban mutum-mutumi wanda ke ba da bayanan kallo-kallo game da mutum-mutumin. Launuka daban-daban da tsarin haskakawa na iya sadar da nasara iri-iri, gazawa, ko jahohi marasa aiki:

LED launi Farin tsaka tsaki jihohi
Green Al'ada Jihohi
Jahohin Tilastawa Jahohin Jahohin Jahohin Jahar Gaggawa

Tsarin LED Solid Pulsing
Kifi sau biyu
Ƙaƙƙarfan Pulsing Pulsing

Matsayin Robot
An kunna shi kuma baya gudanar da yarjejeniya Robot yana aiki (misali, sabunta software ko firmware, kafa tsarin tafiyar da aiki, soke tsarin tafiyar da yarjejeniya) An kammala aikin (misali, ƙayyadaddun yarjejeniya, sabunta software, kayan aikin da aka haɗe ko keɓe) Yarjejeniyar ta cika. An dakatar da yarjejeniya

Kuskuren software

Kifi sau uku, akai-akai

Kuskuren jiki (misali, karon kayan aiki)

Hakanan za'a iya kashe hasken matsayi a cikin saitunan mutum-mutumi.

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BABI NA 3: BAYANIN TSARI
3.2 Pipettes
Opentrons pipettes sune na'urori masu daidaitawa da ake amfani da su don matsar da ruwa a ko'ina cikin wurin aiki yayin aiwatar da ka'idoji. Akwai da yawa Opentrons Flex pipettes, waɗanda zasu iya ɗaukar juzu'i daga 1 µL zuwa 1000 µL a cikin tashoshi 1, 8, ko 96:
Opentrons Flex 1-Channel Pipette (1 µL) Buɗewar Flex 50-Channel Pipette (1 µL) Opentrons Flex 5-Channel Pipette (1000 µL) Opentrons Flex 8-Channel Pipette (1) µL)
Pipettes suna haɗawa da gantry ta amfani da sukurori a gaban pipette. 1-tashar da 8-tashar pipettes kowannensu ya mamaye dutsen pipette daya (hagu ko dama); pipette mai tashar tashoshi 96 ya mamaye duka hawa biyu. Don cikakkun bayanai game da shigar pipettes, duba Shigar Kayan aiki da Calibration.

Abubuwan da aka Kama
Sukurori

Maƙallan Haɗe-haɗe na kama

Mai fitarwa

Nozzles (O-zoben da za a iya maye gurbinsu)

Nozzles (Kafaffen O-zobba)

Wuraren abubuwan da aka haɗa na 1-, 8-, da 96-tashar pipettes.

Mai fitarwa

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BABI NA 3: BAYANIN TSARI

Su pipettes na ɗaukar tukwici na filastik da za a iya zubarwa ta hanyar danna su a kan bututun pipette, sannan a yi amfani da tukwici don nema da watsa ruwa. Adadin jimlar ƙarfin da ake buƙata don ɗauka yana ƙaruwa yayin da ake karɓar ƙarin nasihun lokaci guda. Don ƙananan lambobi na tukwici, pipette yana haɗa tukwici ta hanyar tura kowane bututun pipette zuwa ƙasa. Don cimma ƙarfin da ake buƙata don ɗaukar cikakkun tukwici, pipette mai tashar tashoshi 96 kuma yana jan tukwici zuwa sama kan nozzles. Wannan aikin ja yana buƙatar sanya tulun tip a cikin adaftan tip, maimakon kai tsaye a cikin ramin bene. Don watsar da tukwici (ko mayar da su zuwa rumbun su), injin pipette ejector yana tura tukwici daga cikin nozzles.

Bayanan Pipette
Opentrons Flex pipettes an ƙera su don ɗaukar nau'ikan ƙira. Saboda fa'idarsu gabaɗaya, za su iya amfani da tukwici masu girma dabam, waɗanda ke shafar halayen sarrafa ruwa. Opentrons ya gwada Flex pipettes don daidaito da daidaito a cikin adadin tip da haɗuwar ƙarar ruwa:

Pipette
Flex 1-Channel
50 µL
Flex 1-Channel
1000 µL
Flex 8-Channel
50 µL
Flex 8-Channel
1000 µL

Ƙarfin Tukwici 50 µL 50 µL 50 µL 50 µL 50 µL 200 µL 1000 µL

Gwajin Juzu'i 1 µL 10 µL 50 µL 5 µL 50 µL
200 µL 1000 µL
1 µL 10 µL 50 µL 5 µL 50 µL 200 µL 1000 µL

Daidaiton %D 8.00% 1.50% 1.25% 5.00% 0.50% 0.50% 0.50% 10.00% 2.50% 1.25% 8.00% 2.50% 1.00% 0.70%

Daidaitaccen %CV 7.00% 0.50% 0.40% 2.50% 0.30% 0.15% 0.15% 8.00% 1.00% 0.60% 4.00% 0.60% 0.25% 0.15%

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BABI NA 3: BAYANIN TSARI

Flex 96-Channel
1000 µL

50 µL 50 µL 200 µL 1000 µL

5 µL 50 µL 200 µL 1000 µL

10.00% 2.50% 1.50% 1.50%

5.00% 1.25% 1.25% 1.50%

Rike wannan daidaiton bayanin a zuciyarsa lokacin zabar nasiha don pipette ɗinku. Gabaɗaya, don kyakkyawan sakamako yakamata ku yi amfani da mafi ƙanƙanta nasihun da suka dace da buƙatun ƙa'idar ku.

Lura: Opentrons na yin gwajin juzu'i na Flex pipettes don tabbatar da cewa sun cika daidaito da ƙayyadaddun ƙayyadaddun bayanai da aka jera a sama. Ba dole ba ne ka daidaita ƙarar da pipettes ɗinka ke bayarwa kafin amfani. Dole ne ku yi daidaitawar matsayi kawai. Duba sashe na gaba, da kuma sashin Shigar da Pipette na babin Shigarwa da Matsala, don cikakkun bayanai.
Ayyukan Opentrons Care da Opentrons Care Plus sun haɗa da maye gurbin pipette na shekara da takaddun shaida. Duba sashin Sabis na Sabis na Babin Kulawa da Sabis don cikakkun bayanai.

Pipette calibration
Kit ɗin Mai amfani ya haɗa da binciken daidaita bututun ƙarfe, wanda kuke amfani da shi yayin daidaita matsayi. Yayin gudanar da yarjejeniya, adana binciken lafiya a kan mariƙin maganadisu akan ginshiƙin gaban robot. Yayin aikin daidaitawa, haɗa binciken zuwa bututun da ya dace kuma a kulle shi a wuri. Robot ɗin yana motsa binciken zuwa wuraren daidaitawa a kan bene don auna ainihin matsayin pipette.
Adaftar tip tip
Opentrons Flex 96-tashar pipette jiragen ruwa tare da adaftan tukwici huɗu. Waɗannan su ne madaidaicin kafafan katako na aluminum waɗanda kuke sanyawa a kan bene. Adaftan suna riƙe da Flex 50 L, 200 L, da 1000 µL tip.

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BABI NA 3: BAYANIN TSARI
Saboda ƙarfin da ke tattare da shi, pipette mai tashar tashoshi 96 yana buƙatar adafta don haɗa cikakken rakiyar tukwici yadda ya kamata. A lokacin aikin haɗin gwiwa, pipette yana motsawa a kan adaftan, ya sauke kansa a kan fil ɗin hawa, kuma yana jan tukwici a kan pipettes ta ɗaga adaftan da tip. Jawo tukwici, maimakon turawa, yana ba da damar da ake buƙata don amintaccen tukwici ga pipettes kuma yana hana warping saman bene. Lokacin da aka gama, pipette mai tashar tashoshi 96 yana saukar da adaftar da madaidaicin tukwici a kan bene. Duba sashin Tukwici da tukwici na sashin Labware don ƙarin bayani.
Karɓar ɓangaren tip
Pipette mai tashar tashoshi 96 na iya ɗaukar cikakken tarin tukwici ko ƙaramin adadin tukwici. Wannan yana ƙara yawan aikace-aikacen da za ku iya yi tare da pipette mai tashar tashoshi 96, tun da yake ya mamaye duka biyun pipette.
A halin yanzu, pipette mai tashar tashoshi 96 yana goyan bayan ɗaukar hoto na ɓangarori don tukwici 8 a cikin shimfidar shafi. A cikin wannan saitin, pipette ko dai yana amfani da nozzles ɗin sa na hagu don ɗaukar tukwici dama-zuwa-hagu daga rakiyar tip, ko madaidaicin nozzles ɗinsa na dama don ɗaukar tukwici hagu-zuwa-dama daga taragon tip.
Lokacin ɗaukar ƙasa da tukwici 96 daga tulun tip, dole ne a sanya rak ɗin kai tsaye a kan bene, ba a cikin adaftan tip ɗin ba.
Pipette firikwensin
Opentrons Flex pipettes suna da adadin na'urori masu auna firikwensin da ke ganowa da rikodin bayanai game da matsayin pipette da duk wani tukwici da ya ɗauka.
SENSOR KYAUTA
A haɗe tare da binciken ƙarfe ko tip mai ɗaukar nauyi, na'urori masu auna ƙarfin aiki suna gano lokacin da pipette yayi hulɗa da wani abu. Ana amfani da gano lamba tsakanin binciken ƙarfe da bene a cikin gyare-gyaren pipette mai sarrafa kansa da tsarin daidaita tsarin.
1-tashar pipettes suna da firikwensin capacitance guda ɗaya, yayin da pipettes masu yawa na tashoshi suna da biyu: akan tashoshi 1 da 8 na pipettes na tashoshi 8, kuma akan tashoshi 1 da 96 (matsayi A1 da H12) na 96-tashar pipette.

OPENTRONS FLEX

BABI NA 3: BAYANIN TSARI

HANYOYIN GABATAR DA HANYOYIN GABATARWA
Maɓalli na photointerruptor yana gano matsayin injin fitar da tip ɗin pipette, yana tabbatar da ko an sami nasarar karɓo nasihohi ko jefar. 1-tashar, 8-tashar, da 96-tashar pipettes duk suna da firikwensin gani guda ɗaya wanda ke lura da abin da aka makala a duk tashoshi.
Pipette firmware updates
Opentrons Flex yana sabunta firmware na pipette ta atomatik don kiyaye shi tare da sigar software na robot. Sabunta firmware Pipette yawanci sauri ne, kuma suna faruwa a duk lokacin da:
Kuna haɗa pipette. Robot ya sake farawa.
Idan, saboda kowane dalili, firmware na pipette da nau'ikan software na robot sun daina aiki tare, zaku iya sabunta firmware da hannu a cikin Opentrons App.
1. Danna Na'urori. 2. Danna kan Flex ɗin ku a cikin jerin na'urar. 3. A ƙarƙashin Instruments da Modules, pipette da ba a daidaita ba zai nuna karatun banner na gargaɗi
"An sabunta firmware." Danna Sabuntawa yanzu don fara sabuntawa.
Za ka iya view sigar firmware da aka shigar a halin yanzu na kowane pipette da aka haɗe. A kan allon taɓawa, je zuwa Instruments kuma danna sunan pipette. A cikin Opentrons App, nemo katin pipette a ƙarƙashin Instruments da Modules, danna menu mai dige uku (), sannan danna Game da pipette.

3.3 Gurasa
Mai riko yana motsa labware a duk faɗin wurin aiki da stagyankin yayin aiwatar da ka'idoji. Mai gripper yana haɗawa zuwa tsayin tsayi, wanda ya bambanta da ma'aunin pipette; Ana iya amfani da gripper tare da kowane tsarin pipette. Don cikakkun bayanai kan shigar da gripper, duba Shigar Kayan Kayan aiki da Calibration.
Mai riko na iya matsar da kayan aikin labware a ƙetaren bene da uwa ko kashe na kayan aiki. Mai riko na iya sarrafa wasu cikakkun faranti masu cikakken siket, faranti mai zurfin rijiyar, da tarkace. Don ƙarin cikakkun bayanai kan abin da labware mai riko zai iya motsawa, duba sashin Labware da Opentrons Flex Gripper na sashin Labware, ko tuntubi Laburaren Laburaren Laburaren Opentrons.

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BABI NA 3: BAYANIN TSARI

Bayani dalla-dalla
Muƙamuƙi suna yin motsi na farko na gripper, wanda shine buɗewa ko rufe paddles guda biyu don amfani ko sakin ƙarfi a bangarorin labware. Motsin muƙamuƙi ana sarrafa shi ta injin goga mai lamba 36 VDC wanda aka haɗa da tsarin rak-da-pinion gear.
Don matsar da wani yanki na labware wanda jaws suka kama, gantry yana ɗaga gripper tare da z-axis, yana motsa shi a gefe, sa'an nan kuma ya sauke shi zuwa sabon matsayi na labware.
Wuraren abubuwan da ke cikin gripper.

Abubuwan da aka makala
Calibration fil Jaws Paddles

Gripper calibration
Mai riko ya haɗa da fil ɗin gyaran ƙarfe. Fitin daidaitawa yana cikin wurin ajiya da aka ajiye akan ƙananan ɓangaren abin riko. Magnet yana riƙe fil a wurin. Don cire fil ɗin daidaitawa, kama shi da yatsanka kuma ja a hankali. Don maye gurbin fil, mayar da shi cikin ramin ajiya. Za ku san yana da amintacce lokacin da ya shiga wurin.
Lokacin yin calibrating gripper, haɗa fil zuwa kowane muƙamuƙi bi da bi. Robot ɗin yana motsa fil zuwa wuraren daidaitawa a kan bene don auna ainihin matsayin mai riko.
Yayin gudanar da yarjejeniya, sanya fil a wurin ajiyarsa don kiyayewa. Tuntube mu a support@opentrons.com idan kun rasa fil ɗin daidaitawa.

Gripper firmware updates
Opentrons Flex yana sabunta firmware ta atomatik don kiyaye shi tare da sigar software na robot. Sabunta firmware Gripper yawanci sauri ne, kuma yana faruwa a duk lokacin da:

OPENTRONS FLEX

BABI NA 3: BAYANIN TSARI
Kuna haɗa abin riko. Robot ya sake farawa.
Idan, saboda kowane dalili, gripper firmware da nau'ikan software na robot sun daina aiki tare, zaku iya sabunta firmware da hannu a cikin Opentrons App.
1. Danna Na'urori. 2. Danna kan Flex ɗin ku a cikin jerin na'urar. 3. Ƙarƙashin Instruments da Modules, gripper wanda ba ya aiki tare zai nuna karatun banner na gargadi
"An sabunta firmware." Danna Sabuntawa yanzu don fara sabuntawa.
Za ka iya view sigar firmware da aka shigar a halin yanzu na gripper. A kan allon taɓawa, je zuwa Instruments kuma danna gripper. A cikin Opentrons App, nemo katin gripper a ƙarƙashin Instruments da Modules, danna menu mai dige uku (), sannan danna Game da gripper.
3.4 Tsayawa Tsaida Gaggawa
Pendant Tsaida Gaggawa (E-stop) maɓalli ne na kayan aikin sadaukarwa don dakatar da motsin robot. Opentrons Flex yana buƙatar E-stop mai aiki mai aiki, wanda za a haɗa shi a kowane lokaci. Lokacin da ka danna maɓallin tsayawa, Flex yana soke duk wata yarjejeniya mai gudana ko saitin aiki da sauri da sauri kuma yana hana yawancin motsin mutum-mutumi.
Lokacin amfani da E-Stop
Kuna iya buƙatar danna E-Stop:
Lokacin da akwai kusantar rauni ko lahani ga mai amfani. Lokacin da akwai kusantar lahani ga mutum-mutumi ko wasu kayan masarufi. Lokacin samples ko reagents suna cikin haɗarin kamuwa da cuta. Bayan wani karo na hardware.
Da kyau kada ka taɓa danna E-stop (sai dai lokacin gwajin ingancin kayan aiki da yawa).
Kar a yi amfani da E-stop don soke ayyukan al'ada, da ake tsammani. Madadin haka, yi amfani da maɓallin software akan allon taɓawa ko a cikin Opentrons App. Tsayawa ta hanyar software zai baka damar ci gaba ko soke yarjejeniya, yayin da danna E-stop koyaushe yana soke yarjejeniya nan da nan.

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BABI NA 3: BAYANIN TSARI

Shiga da sakin E-Stop
E-Stop yana da tsarin latsa-zuwa-shiga, karkatarwa-zuwa-saki.
Shiga: Matsa ƙasa da ƙarfi akan maɓallin ja. Flex zai shiga jihar da aka tsaya. Gyara: Da zarar an tsaya, a amince da magance duk wata matsala a wurin aiki, kamar share zubewa,
cire labware, ko motsa gantry (ya kamata ya motsa cikin yardar kaina da hannu). Saki: Juya maɓallin kewayawa agogo. Zai tashi har zuwa matsayin da ba a kwance ba. Sake saitin: A kan allon taɓawa ko a cikin Opentrons App, tabbatar da cewa kun shirya don Flex ya ci gaba
motsi. Gantry zai koma matsayinsa na gida kuma aikin module zai ci gaba.
A cikin jihar da aka dakatar, Flex da kayan aikin da aka haɗa za su kasance kamar haka:

Hardware Gantry Pipettes
Gripper
Module mai zafi-Shaker
Yanayin Zazzabi Module Thermocycler Module Matsayin Hasken taɓawa

Hali
An dakatar da motsi a kwance mai sarrafa kansa. An ba da izinin motsi a kwance da hannu.
An dakatar da motsin pipettes a tsaye. Birki na motar akan gatura a tsaye an haɗa shi don hanawa
pipettes daga fadowa. An dakatar da motsin ƙwanƙwasa da ɗaukar tsinke.
An dakatar da motsi a tsaye na riko. The motor birki a tsaye axis an tsunduma a hana
gripper daga fadowa. Motocin muƙamuƙi waɗanda ke yin ƙarfi sun kasance suna aiki, don haka
gripper ba zai sauke labware da zai iya ɗauka ba.
Girgizawa ta tsaya da gidaje. Latch ɗin labware yana buɗewa. An kashe dumama
An kashe dumama ko sanyaya.
An kashe dumama ko sanyaya.
Hasken yayi ja.
Saƙon sokewa yana ɗaukar allon. Alamun kan allo yana nuna lokacin da kuka yi nasara
kashe maɓallin tsayawa.

OPENTRONS FLEX

BABI NA 3: BAYANIN TSARI
3.5 Haɗi

Kunnawa/Kashe Canjawa

Rufin Side

USB-A Ports

IEC Power Inlet

Tashar jiragen ruwa AUX-1, AUX-2, USB-B, Ethernet

Haɗin wutar lantarki
Opentrons Flex yana haɗi zuwa tushen wuta ta madaidaicin mashigan IEC-C14. Robot ɗin ya ƙunshi wutar lantarki mai cikakken kewayon AC/DC na ciki, yana karɓar 100 VAC, shigarwar 240/50 Hz kuma yana canza shi zuwa 60 VDC. Duk sauran na'urorin lantarki na ciki suna da ƙarfi ta hanyar 36 VDC wadata.

Gargaɗi: Yi amfani da igiyar wutar lantarki da aka bayar tare da mutum-mutumi. Kar a yi amfani da igiyar wuta tare da rashin isassun halin yanzu ko voltage ratings.
Kiyaye igiyar wutar ba tare da cikas ba don haka zaka iya cire ta idan ya cancanta.

Akwai kuma batirin tantanin halitta na tsabar kudin CR1220 da zai iya kunna agogon robot a ainihin lokacin lokacin da ba a haɗa shi da wutar lantarki ba. Baturin yana cikin madaidaicin allon taɓawa. Tuntuɓi Tallafin Opentron don ƙarin bayani idan kuna tunanin kuna buƙatar maye gurbin baturin.

OPENTRONS FLEX

BABI NA 3: BAYANIN TSARI
USB da haɗin haɗin gwiwa
Opentrons Flex yana da jimlar tashoshin USB guda 10 waɗanda ke cikin yankuna daban-daban na robot, waɗanda ke ba da dalilai daban-daban.
Tashar jiragen ruwa na 8 na baya na USB-A (wanda aka lasafta USB-1 ta hanyar USB-8) da 2 ƙarin tashoshin jiragen ruwa (Masu haɗin M12 masu lamba AUX-1 da AUX-2) don haɗa nau'ikan Opentrons da kayan haɗi. Duba babin Modules don ƙarin bayani kan haɗa waɗannan na'urori da amfani da su a cikin ka'idojin ku. Babban tashar USB-B na baya shine don haɗa robot ɗin zuwa kwamfutar tafi-da-gidanka ko kwamfutar tebur, don kafa sadarwa tare da Opentrons App da ke aiki akan kwamfutar da aka haɗa. Tashar tashar USB-A ta gaba (USB-9), wacce ke ƙasa da allon taɓawa, tana da ayyuka iri ɗaya da tashoshin USB-A na baya.
Lura: Tashoshin USB suna da iyakacin ƙarfi don kare mutum-mutumi da na'urorin da aka haɗa. Ana rarraba isar da wutar lantarki a ciki zuwa ƙungiyoyin tashar jiragen ruwa guda uku: na baya na USB-A tashar jiragen ruwa (USB-1 ta hanyar USB-4), tashar USB-A ta dama (USB-5 ta USB-8), da USB-A na gaba. tashar jiragen ruwa. Kowane ɗayan waɗannan rukunin zai sadar da iyakar 500mA zuwa na'urorin USB 2.0 masu jituwa da aka haɗa.
Hanyoyin sadarwa
Opentrons Flex na iya haɗawa zuwa cibiyar sadarwar yanki ta hanyar haɗin waya (Ethernet) ko mara waya (Wi-Fi).
Tashar tashar Ethernet tana kan bayan mutum-mutumin. Haɗa shi zuwa cibiyar Ethernet ko kunna hanyar sadarwar ku. Ko, farawa daga tsarin tsarin mutum-mutumi 7.1.0, haɗa shi kai tsaye zuwa tashar Ethernet a kan kwamfutarka. Tsarin Wi-Fi na ciki yana goyan bayan cibiyoyin sadarwa 802.11 ac/a/b/g/n tare da eriya mai dual-band 2.4/5 GHz.

OPENTRONS FLEX

BABI NA 3: BAYANIN TSARI

3.6 Bayanin tsarin

Gabaɗaya ƙayyadaddun bayanai

Matsakaicin Ma'aunin nauyi
Kariyar tabawa
Wi-Fi Ethernet USB
Shigar da wutar lantarki ta kamara
Main kayan aiki voltage Sauyawar Mains samar da mitar juyi tsarin Rarraba Gajerun kewayawa wadata na yanzu Frame abun da ke ciki Window Bukatun samun iska

87 × 69 × 84 cm / 34.25 × 27 × 33 a cikin (W, D, H)
88.5 kg / 195 lb 12 ANSI/ SLAS masu jituwa ramummuka a wurin aiki
(mai isa ga pipettes) ƙarin ramummuka 4 don staging tips da labware
(mai samuwa kawai ga gripper) 7-inch LCD touchscreen tare da karce- da lalacewa-resistant Gorilla Glass 3
802.11 ac/a/b/g/n dual-band (2.4/5 GHz)
100 Mbps 9 USB-A tashar jiragen ruwa 1 USB-B tashar jiragen ruwa USB 2.0 gudun
2MP, hoto da bidiyo 100 VAC, 240 Hz, 50 60 A/1 VAC, 4.0 A/115 VAC
± 10%
± 5%
TN-S
6.3 A
M karfe da CNC aluminum zane
Tagar gefen polycarbonate mai cirewa da ƙofar gaba Aƙalla 20 cm / 8 a tsakanin naúrar da bango

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Abubuwan buƙatun PC masu haɗawa

Opentrons App yana gudana akan: Windows 10 ko daga baya macOS 10.10 ko daga baya Ubuntu 12.04 ko kuma daga baya.

Bayanan muhalli

Yanayin muhalli Matsayin zafin yanayi Matsayin gurɓataccen yanayi

Amfani na cikin gida kawai +20 zuwa +25 °C (an shawarta) 40%, mara sanyaya (shawarar) 60 (ƙazanta mara amfani kawai)

Don ƙarin bayani kan karɓaɓɓen yanayin muhalli don amfani da sufuri, duba sashin Yanayin Muhalli na babin shigarwa da ƙaura.

Takaddun shaida

Takaddun shaida sun cika Ba a tantance su ba

CE, ETL, FCC, ISO 9001 IVD, GMP

Ana buga taƙaitaccen bayanin takaddun shaida akan sitika a bayan Flex, kusa da kunnawa/kashe. Don cikakkun takaddun shaida da bayanin yarda, duba sashin Yarda da Ka'ida a cikin Gabatarwa.

Serial number
Kowane Flex yana da lambar serial na musamman. Tsarin lambar serial ɗin yana ba da ƙarin bayani, gami da kwanan watan samarwa na robot. Domin misaliample, lambar serial FLXA1020231007001 zai nuna:

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Bayanan Bayani na FLX A10 2023 10 07 001

Rukunin Ranar Watan Shekarar Nau'in Model

Ma'ana Robot shine Opentrons Flex. Lambar don sigar samar da robot. An kera wannan mutum-mutumin ne a shekarar 2023. An yi na’urar a watan Oktoba. An kera na'urar robot din ne a ranar 7 ga wata. Lamba na musamman don mutummutumi da aka yi a wata rana.

Kuna iya nemo serial number don Flex ɗin ku:
A kan siti na takaddun shaida a bayan Flex, kusa da kunnawa/kashe. A gefen baya na allon taɓawa (zuwa wurin aiki). A cikin Opentrons App a ƙarƙashin Na'urori> Flex ɗinku> Saitunan Robot> Na ci gaba.

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Modules
Opentrons Flex yana haɗawa tare da adadin kayan aikin Opentrons. Duk nau'ikan nau'ikan nau'ikan nau'ikan kayan aiki ne waɗanda ke mamaye ramummukan bene, kuma galibi ana sarrafa su ta hanyar haɗin kebul na USB.
Wannan babin yana bayyana ayyuka da ƙayyadaddun bayanai na zahiri na kayayyaki waɗanda suka dace da tsarin Opentrons Flex, da yadda ake haɗawa da daidaita su. Don ƙarin cikakkun bayanai kan saitin module da amfani, tuntuɓi littafin jagora don kowane nau'ikan. Don cikakkun bayanai kan haɗa na'urori a cikin ka'idojin ku, duba sashin Mai tsara yarjejeniya na babin Haɓaka yarjejeniya ko takaddun API ɗin layi na Python Protocol.
4.1 Moduloli masu goyan baya
Opentrons Flex ya dace da nau'ikan nau'ikan nau'ikan Opentrons na kan bene:
Module na Heater-Shaker yana ba da dumama kan bene da girgiza orbital. Za'a iya mai da module ɗin zuwa 95 ° C, kuma yana iya girgiza sampdaga 200 zuwa 3000 rpm.
Block Magnetic na'ura ce mai wuce gona da iri wacce ke rike da kayan aikin labware kusa da babban karfinsa na neodymium maganadiso. Module Magnetic na OT-2 GEN1 da GEN2, waɗanda ke matsar da maganadisu sama da ƙasa dangane da labware, ba su da tallafi akan Opentrons Flex.
Module na Zazzabi na'urar faranti ne mai zafi da sanyi wanda ke da ikon kiyaye yanayin zafi tsakanin 4 zuwa 95 ° C.
Module na Thermocycler yana ba da kan bene, cikakken sarrafa zafin jiki mai sarrafa kansa, yana ba da damar aiki da kai na matakai na sama da ƙasa. Thermocycler GEN2 ya dace da mai riko. Ba za a iya amfani da Thermocycler GEN1 tare da gripper ba, don haka ba a samun tallafi akan Opentrons Flex.
Wasu na'urori waɗanda aka tsara asali don OT-2 sun dace da Flex, kamar yadda aka taƙaita a teburin da ke ƙasa. Alamar dubawa tana nuna dacewa, kuma X yana nuna rashin jituwa.

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Nau'in na'ura da tsara Module mai zafi-Shaker GEN1 Magnetic Module GEN1 Magnetic Module GEN2 Magnetic Block GEN1 Zazzabi Module

OT-2

sassauƙa

4.2 Module caddy tsarin
An ƙirƙira na'urori masu jituwa masu jituwa don dacewa da kuɗaɗe waɗanda ke mamaye sarari a ƙasan bene. Wannan tsarin yana ba da damar labware a saman na'urori su kasance kusa da saman bene, kuma yana ba da damar yin jigilar kebul na ƙasan bene don haka belin ya kasance cikin tsabta yayin aiwatar da yarjejeniya.

Caddies don Heater-Shaker, Zazzabi, da Modulolin Thermocycler.

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Don dacewa da wani module a saman bene, dole ne a fara sanya shi a cikin madaidaicin ma'auni. Kowane nau'in nau'i mai jituwa yana da nasa ƙirar ƙira wanda ya daidaita ƙirar da labware daidai da bene da ke kewaye. ( Banda shi ne Magnetic Block, wanda baya buƙatar wutar lantarki ko kebul na USB don haka yana zaune kai tsaye a saman bene.) Ana iya sanya Caddies don kayayyaki waɗanda ke mamaye ramin guda ɗaya a ko'ina cikin shafi na 1 ko 3; Thermocycler za a iya sanya shi a cikin ramummuka A1 da B1 a lokaci guda.
Gabaɗaya, don shigar da module caddy:
1. Cire duk wani ramummuka na bene daga wurin da tsarin zai tafi. 2. Zama module ɗin a cikin ɗigon sa kuma ku ƙara matsawa. 3. Rarraba ikon module da kebul na USB ta cikin murfin gefe, sama ta cikin rami mara kyau, kuma
haɗa su zuwa tsarin. 4. Zama ɗigon module a cikin ramin kuma murƙushe shi a wuri.
Don ainihin umarnin shigarwa, tuntuɓi Jagorar Quickstart ko Manual Umarni don takamaiman ƙirar. Haɗin kebul da hanyar haɗewa zuwa caddy sun bambanta ta module.
4.3 Module daidaitawa
Lokacin da kuka fara shigar da module akan Flex, kuna buƙatar gudanar da daidaitawar matsayi na atomatik. Wannan tsari yayi kama da daidaitawar matsayi don kayan aiki, kuma yana tabbatar da cewa Flex yana motsawa zuwa daidaitattun wurare don ingantaccen aikin ƙa'ida. A lokacin daidaitawa, Flex zai matsa zuwa wurare akan adaftan daidaitawa, wanda yayi kama da murabba'in daidaitawa waɗanda ke ɓangaren ramukan bene mai cirewa.

Adaftar daidaitawa don Heater-Shaker, Zazzabi, da Modulolin Thermocycler.
Ana buƙatar gyare-gyaren ƙirar ƙira don duk nau'ikan da ke shigarwa ta hanyar caddy: Heater-Shaker, Temperature, da Modules na Thermocycler. Toshe Magnetic baya buƙatar daidaitawa, kuma yana shirye don amfani da zaran kun sanya shi akan bene.

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Lokacin da za a daidaita modules
Flex yana motsa ku ta atomatik don yin gyare-gyare lokacin da kuka haɗa da kunna wuta akan tsarin da bashi da kowane bayanan daidaitawa da aka adana. (Zaku iya watsi da wannan faɗakarwa, amma ba za ku iya gudanar da ladabi tare da tsarin ba har sai kun daidaita shi.)
Da zarar kun gama daidaitawa, Flex yana adana bayanan daidaitawa da lambar serial ɗin module don amfani nan gaba. Flex ba zai sa ku sake daidaitawa ba sai dai idan kun share bayanan daidaitawa na wannan tsarin a cikin saitunan mutum-mutumi. Kuna iya kunnawa da kashe tsarin ku kyauta, ko ma matsar da shi zuwa wani ramin bene, ba tare da buƙatar sake daidaitawa ba. Idan kuna son sake daidaitawa, zaku iya fara aiwatar da kowane lokaci daga katin ƙirar a cikin Opentrons App. (Ba a samun recalibration daga allon taɓawa.)
Yadda ake daidaita modules
Umarnin akan allon taɓawa ko a cikin Opentrons App zai jagorance ku ta hanyar daidaitawa. Gabaɗaya matakan sune:
1. Tattara kayan aikin da ake buƙata, gami da adaftar daidaitawa na module da binciken daidaitawar pipette. 2. Sanya adaftar daidaitawa a kan saman module kuma tabbatar da cewa matakin gaba daya ne.
Wasu kayayyaki na iya buƙatar ka ɗaure adaftar zuwa tsarin. 3. Haɗa bincike na daidaitawa zuwa pipette. 4. Flex zai matsa kai tsaye don taɓa wasu maki akan adaftar daidaitawa kuma ya adana waɗannan
ƙimar daidaitawa don amfani na gaba.
Da zarar an gama daidaitawa kuma kun cire adaftar da bincike, tsarin zai kasance a shirye don amfani a cikin ladabi.
A kowane lokaci, zaku iya view kuma sarrafa bayanan daidaita tsarin ku a cikin Opentrons App. Je zuwa Saitunan Robot don Flex ɗin ku kuma danna kan shafin Calibration.

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4.4 Module mai zafi-Shaker GEN1

Heater-Shaker fasali
DUMI-DUMINSU DA KYAUTA
Heater-Shaker yana ba da dumama kan bene da girgiza orbital. Za'a iya mai da module ɗin zuwa 95 ° C, tare da yanayin zafin jiki mai zuwafile:
Yanayin zafin jiki: 37 °C Daidaitaccen zafin jiki: ± 95 °C a 0.5 °C Daidaitawar yanayin zafi: ± 55 °C a 0.5 ° CRamp Yawan: 10 °C/min
Module na iya girgiza samples daga 200 zuwa 3000 rpm, tare da mai girgiza profile:
Diamita na Orbital: 2.0 mm Jagoran Orbital: Kewayon saurin agogo: 200 rpm Daidaitaccen sauri: ± 3000 rpm
Samfurin yana da madaidaicin labware mai ƙarfi don adana faranti zuwa tsarin kafin girgiza.

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THERMAL ADAPTERS Ana buƙatar adaftar thermal mai dacewa don ƙara labware zuwa Heater-Shaker. Ana iya siyan adaftar kai tsaye daga Opentrons a https://shop.opentrons.com. Abubuwan Adaftar thermal a halin yanzu sun haɗa da:

Universal Flat Adafta

Adaftar PCR

Adaftar Rijiyar Zurfi

96 Flat Bottom Adafta

Sarrafa SOFTWARE
Heater-Shaker yana da cikakken shirye-shirye a cikin Protocol Designer da Python Protocol API. Python API kuma yana ba da damar yin wasu matakan ƙa'idodi a layi daya yayin da Heater-Shaker ke aiki. Dubi umarnin da ba a toshewa a cikin takaddun API don cikakkun bayanai kan ƙara matakan layi ɗaya zuwa ƙa'idodin ku.
A waje da ka'idoji, Opentrons App na iya nuna halin yanzu na Heater-Shaker kuma yana iya sarrafa hita, shaker, da latch ɗin labware kai tsaye.

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Bayani dalla-dalla na Heater-Shaker

Girman Ma'aunin nauyi Module shigar wutar lantarki shigarwar adaftar wutar lantarki Main samar da wutar lantarki voltage sauye-sauyen Overvoltage Amfani da wutar lantarki

152 × 90 × 82 mm (L/W/H) 1.34 kg 36 VDC, 6.1 A 100 VAC, 240/50 Hz ± 60% Category II Rago: 10 W

Yawanci: girgiza: 4 W dumama: 11 W Dumama da girgiza: 10 W

Yanayin Muhalli Matsayin yanayin zafi Dangantakar zafi Matsayin gurɓataccen yanayi

Matsakaicin: 125 W Amfani na cikin gida kawai 130 °C Har zuwa 20%, mara ƙarfi Har zuwa 25 m sama da matakin teku 80

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4.5 Magnetic Block GEN1

Fasalolin Magnetic Block
Opentrons Magnetic Block GEN1 shine mariƙin rijiyar magnetic 96. Ana amfani da tubalan Magnetic a cikin ƙa'idodin da suka dogara da maganadisu don cire barbashi daga dakatarwa da riƙe su a cikin faranti rijiyoyin yayin wankewa, kurkure, ko wasu hanyoyin haɓakawa. Don misaliample, shirye-shiryen NGS mai sarrafa kansa; tsarkake genomic da mitochondrial DNA, RNA, ko sunadarai; da sauran hanyoyin hakar duk lokuta ne na amfani da zasu iya haɗa da tubalan maganadisu.
KAYAN MAGANAR
Toshe Magnetic ba shi da ƙarfi, baya ƙunshe da kowane kayan lantarki, kuma baya matsar da beads sama ko ƙasa cikin bayani. Rijiyoyin sun ƙunshi maganadiso na zoben neodymium mai ƙarfi 96 da aka gyara a kan gadon da aka ɗora a bazara, wanda ke taimakawa kiyaye juriya tsakanin toshe da pipettes yayin gudanar da ƙa'idodi na atomatik.
Sarrafa SOFTWARE
Magnetic Block GEN1 cikakke ne wanda za'a iya tsara shi a cikin Mai tsara yarjejeniya da Python Protocol API.

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A waje da ka'idoji, duk da haka, allon taɓawa da Opentrons App ba su da masaniya kuma ba za su iya nuna halin yanzu na Magnetic Block GEN1 ba. Wannan ƙirar mara ƙarfi ce. Ba ya ƙunshi kayan lantarki ko na inji waɗanda zasu iya sadarwa tare da mutum-mutumin Flex. Kuna "sarrafa" Block na Magnetic ta hanyar ladabi waɗanda ke amfani da Opentrons Flex Gripper don ƙarawa da cire labware daga wannan tsarin.

Magnetic Block ƙayyadaddun bayanai

Girman Ma'aunin Ma'aunin Module Ikon Magnet Matsayin Muhalli Yanayin yanayi Yanayin zafi Dangantakar zafi Matsayin Matsayin gurɓataccen digiri

136 × 94 × 45 mm (L/W/H) 1.13 kg Babu, module ba shi da iko N52 neodymium Amfani na cikin gida kawai 20 °C 25%, mara sanyaya Har zuwa 30 m sama da matakin teku 80

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4.6 Module Zazzabi GEN2

Fasalolin Modulun Zazzabi
DUFA DA SANYI
Module Zazzabi na Opentrons GEN2 ƙirar faranti ne mai zafi da sanyi. Ana amfani da shi sau da yawa a cikin ladabi waɗanda ke buƙatar dumama, sanyaya, ko canjin yanayin zafi. Na'urar zata iya kaiwa da kula da yanayin zafi daga 4 °C zuwa 95 °C cikin mintuna, ya danganta da tsarin tsarin da abun ciki.
KASHE-KASHEN THERMAL
Don riƙe labware a zafin jiki, ƙirar tana amfani da tubalan thermal na aluminum. Module ɗin ya zo tare da 24well da 96-rijiyoyin thermal blocks. Module na zafin jiki ya zo tare da toshe mai zurfi mai zurfi da shingen ƙasa lebur wanda aka tsara don amfani tare da Flex Gripper. Tubalan suna riƙe da bututun 1.5 ml da 2.0 ml, faranti 96 mai kyau PCR, tube PCR, faranti mai zurfi, da faranti na ƙasa lebur.

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Lura: Hakanan tsarin yana jigilar kaya tare da toshe ƙasa mai lebur don OT-2. Kada kayi amfani da toshe OT-2 tare da Flex. Ƙaƙwalwar ƙasa na Flex yana da kalmomin "Opentrons Flex" a saman samansa. Nau'in na OT-2 bai yi ba.

24- rijiyar thermal block

96- rijiyar thermal block

Zurfafa rijiyar thermal block

Lebur ƙasa thermal block don Flex

WANKAN RUWA DA ZUMUNCI
Saboda iska shine insulator mai kyau na thermal, rata tsakanin labware da toshewar thermal na iya rinjayar aikin-lokaci-lokaci na Module Zazzabi. Sanya ruwa kadan a cikin 24- ko 96-rijiyoyin thermal tubalan yana kawar da gibin iska kuma yana inganta aikin dumama. Madaidaicin adadin ruwa ya dogara da toshe thermal da labware. Duba Farar Takarda Module Temperatuur don ƙarin shawarwari.

Sarrafa SOFTWARE
Module Yanayin Zazzabi cikakke ne da za a iya tsara shi a cikin Mai tsara yarjejeniya da API ɗin Protocol na Python.
A waje da ka'idoji, Opentrons App na iya nuna halin yanzu na Module Zazzabi kuma yana iya sarrafa zafin farantin saman kai tsaye.

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Ƙayyadaddun Modulun Zazzabi

Girman Module Nauyin Ƙarfin
Yanayin Muhalli Matsayin yanayin zafi Dangantakar zafi Matsayin gurɓataccen yanayi

194 × 90 × 84 mm (L/W/H) 1.5 kg Shigarwa: 100 VAC, 240/50 Hz, 60 A Fitarwa: 4.0 VDC, 36 A, 6.1 W max Amfani na cikin gida kawai <219.6 °C (an shawarta don mafi kyawun sanyaya ) Har zuwa 22%, ba mai narkewa Har zuwa 60 m sama da matakin teku 2000

4.7 Module na Thermocycler GEN2

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Fasalolin Thermocycler
Module na Opentrons Thermocycler Module GEN2 cikakken mai sarrafa kayan zafi ne akan bene, yana samar da PCR mara hannu a tsarin farantin rijiyar 96. Zafin murfinsa da hatimin da za a iya zubarwa sun yi daidai da farantin, yana tabbatar da ingantaccen sample dumama da kadan evaporation.
DUMA DA SANYA Tushewar Thermocycler na iya zafi da sanyi, kuma murfinsa na iya zafi, tare da ƙarin zafin jiki mai zuwa.file: Thermal block zafin jiki kewayon: 4 °C Thermal block iyakar dumama ramp Darajar: 4.25 ° C / s daga GEN2 na yanayi zuwa 95 ° C Thermal block matsakaicin sanyaya ramp kudi: 2.0 °C/s daga 95 °C zuwa na yanayi kewayon zafin jiki na Lid: 37 °C Daidaitaccen zafin jiki: ± 110 °C Ana iya buɗe murfin mai sarrafa kansa kamar yadda ake buƙata yayin aiwatar da yarjejeniya.
Bayani: THERMOCYCLER PROFILES Thermocycler na iya aiwatar da profiles: yin keke ta atomatik ta hanyar jerin toshe yanayin zafi don aiwatar da halayen zafi.
RUBBER AAUTOMATION SEALS Thermocycler yana zuwa tare da hatimin sarrafa roba don taimakawa rage ƙawancewar ruwa. Kowane hatimi dole ne a haifuwa kafin amfani kuma ana iya amfani dashi don gudu da yawa. Ana iya siyan ƙarin hatimai kai tsaye daga Opentrons a https://shop.opentrons.com.
Sarrafa SOFTWARE Thermocycler yana da cikakken shirye-shirye a cikin Mawallafin Protocol da Python Protocol API. A waje da ka'idoji, Opentrons App na iya nuna halin yanzu na Thermocycler kuma yana iya sarrafa zafin toshe kai tsaye, zafin murfi, da matsayin murfi.

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Bayani dalla-dalla na Thermocycler

Girma (buɗe murfi) Girma (rufe rufe) Nauyi (ciki har da bututun baya) Adaftar wuta vol.tage Power adaftar halin yanzu Overvoltage Yanayin Muhalli Yanayin zafi Dangin zafi Matsayin buƙatun samun iska

244.95 × 172 × 310.1 mm (L/W/H) 244.95 × 172 × 170.35 mm (L/W/H) 8.4 kg 100 V a 240/50 Hz 60 A Category II Amfani cikin gida kawai 8.5 °C 5 °C (an yarda) 20%, ba mai haɗawa Har zuwa 25 m sama da matakin teku Aƙalla 2 cm / 40 a tsakanin naúrar da bango

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Labware
Wannan babin ya ƙunshi abubuwa a cikin Laburaren Labware na Opentrons da za ku iya amfani da su tare da Opentrons Flex da Opentrons Flex Gripper. Hakanan yana rufe labware na al'ada kuma, ga masu amfani da wutar lantarki, yana haɗa abubuwan haɗin labware zuwa JSON masu dacewa file ma'anoni.
Kuna iya siyan labware daga masana'antun kayan aiki na asali ko daga shagon Opentrons a https://shop.opentrons.com. Kuma, Opentrons koyaushe yana aiki don tabbatar da sabbin ma'anar labware. Duba Laburaren Labware (wanda aka haɗa a sama) don sabbin jeri.
5.1 Ra'ayoyin Labware
Labware ya ƙunshi fiye da abubuwan da aka sanya a kan bene kuma ana amfani da su a cikin yarjejeniya. Don taimaka muku fahimtar labware na Opentrons, bari mu bincika wannan batu ta fuskoki uku daban-daban. Don Opentrons Flex, labware ya haɗa da abubuwa a cikin Laburaren Laburaren mu, bayanan da ke bayyana kowane yanki na labware, da labware na al'ada.
Labware a matsayin hardware
Laburaren Labware ya ƙunshi duk abin da zaku iya amfani da shi ta tsohuwa tare da Opentrons Flex. Waɗannan abubuwa ne masu dorewa da abubuwa masu amfani waɗanda kuke aiki da su, sake amfani da su, ko jefar yayin aiwatar da yarjejeniya. Ba kwa buƙatar ɗaukar kowane matakai na musamman don aiki tare da abubuwan da ke cikin Laburaren Labura. Robot Flex ya san yadda ake aiki da komai a cikin ɗakin karatu ta atomatik.
Labware kamar bayanai
Ana adana bayanan Labware a cikin bayanin abubuwan Javascript (JSON) files da .json file kari. A JSON file ya haɗa da girman sararin samaniya (tsawo, faɗi, tsayi), ƙarfin juzu'i (L, mL), da sauran ma'auni waɗanda ke ayyana fasalulluka, sifofinsu, da wurare. Lokacin gudanar da yarjejeniya, Flex yana karanta waɗannan .json files don sanin abin da labware yake a kan bene da yadda ake aiki da shi.

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Labware na al'ada
Labware na al'ada labware ne wanda ba a haɗa shi a cikin Laburaren Labura ko labware ne wanda Mahaliccin Labware na Custom ya ƙirƙira. Koyaya, wani lokacin ra'ayin labware na al'ada yana zuwa da nauyi ta ra'ayi na rikitarwa, kuɗi, ko wahala. Amma, labware na al'ada bai kamata ya zama da wahala a fahimta ko ƙirƙira ba. Bari mu ɗan ɗauki ɗan lokaci don buɗe manufar labware na al'ada.
A matsayin exampHar ila yau, Laburaren Laburaren Labware na Opentrons ya ƙunshi faranti 96-rijiya (200 L) daga Corning da BioRad, amma sauran masana'antun suna yin waɗannan faranti ma. Kuma, godiya ga ƙa'idodin masana'antu da aka saba yarda da su, bambance-bambancen da ke tsakanin waɗannan abubuwan laburaren ko'ina ƙanana ne. Koyaya, talakawan 200 L, farantin rijiyar 96 daga Stellar Scientific, Oxford Lab, ko Krackler Scientific (ko duk wani mai siyar da wannan lamarin) shine “Labware na al’ada” don Flex saboda ba a riga an bayyana shi a cikin Laburaren Labware na mu ba. . Bugu da ƙari, ƙananan bambance-bambance a cikin ma'auni na labware na iya yin tasiri sosai kan nasarar tafiyar da ka'idar. Don wannan dalili, yana da mahimmanci a sami cikakkiyar ma'anar labware ga kowane labware da kuke son amfani da shi a cikin ƙa'idar ku.
Hakanan, yayin da labware na al'ada na iya zama abin ɓoyewa, yanki ɗaya na kit, mafi yawan lokaci kawai tukwici, faranti, bututu, da racks da ake amfani da su kowace rana a cikin labs a duk faɗin duniya. Bugu da ƙari, kawai bambanci tsakanin labware na Opentrons da labware na al'ada shine sabon abu ba a riga an bayyana shi a cikin software da ke ba da ikon robot. Flex na iya, kuma ya aikata, aiki tare da wasu kayan aikin labware na asali ko wani abu na musamman, amma kuna buƙatar yin rikodin halayen abin a cikin ma'anar labware JSON. file kuma shigo da wannan bayanan cikin Opentrons App. Dubi sashin Ma'anar Labware na Musamman a ƙasa don ƙarin bayani.
Don taƙaitawa, labware ya haɗa da:
Komai a cikin Laburaren Labware na Opentrons. Ma'anar Labware: bayanai a cikin JSON file wanda ke bayyana siffofi, girma, da iyawar abubuwa ɗaya
kamar faranti, tukwici, tafki, da dai sauransu. Labware na al'ada, waɗanda abubuwa ne waɗanda ba a haɗa su a cikin Laburaren Labura.
Bayan reviewA cikin waɗannan mahimman ra'ayoyi, bari mu bincika nau'ikan da abubuwa a cikin Laburaren Laburaren Opentrons. Bayan haka, za mu gama babin da kariview daga cikin abubuwan da ke cikin bayanan labware file da taƙaita fasali da sabis na Opentrons waɗanda ke taimaka muku ƙirƙirar labware na al'ada.

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5.2 Tafkunan ruwa
Opentrons Flex yana aiki ta tsohuwa tare da tafki guda- da rijiyoyi da yawa da aka jera a ƙasa. Yin amfani da waɗannan tafkunan tana taimakawa rage nauyin aikin ku na shirye-shiryen saboda suna shirye-shiryen sarrafa kansa kai tsaye daga cikin akwatin. Hakanan ana samun bayanin tafki a cikin Opentrons Labware Library.

Tafkunan rijiyoyin guda daya

Ƙayyadaddun Ƙira

Agilent

290 ml V kasa

Axygen

90 ml ruwan zafi

NEST

195 ml ruwan zafi

290 ml V kasa

API load name
agilient_1_ tafki_290ml
axygen_1_ reservoir_90ml
nest_1_ tafki_195ml
nest_1_ tafki_290ml

Rijiyoyi masu yawa

Ƙayyadaddun Ƙira

NEST

12 rijiyoyi 15 ml/ rijiyar V kasa

Amurka Kimiyya

12 rijiyoyi 22 ml/ rijiyar V kasa

Sunan lodin API nest_12_ reservoir_15ml
usascientific_12_ reservoir_22ml

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Ma'anar tafki da API
Laburaren Laburaren Labware na Opentrons yana bayyana halayen tafkunan da aka jera a sama a JSON daban files. Robot da Opentrons Python API sun dogara da waɗannan ma'anar JSON don aiki tare da labware da ka'idojin ku ke amfani da su. Don misaliampHar ila yau, lokacin aiki tare da API, aikin ProtocolContext.load_labware yana karɓar waɗannan sunayen labware azaman ingantattun sigogi a lambar ku. Sunayen lodin API masu alaƙa suna haɗawa da ma'anar ma'anar labware na tafki a cikin ma'ajin Opentrons GitHub.
Labware na tafki na al'ada
Gwada ƙirƙirar ma'anar labware na al'ada tare da Opentrons Labware Creator idan ba a jera tafki da kuke son amfani da shi anan ba. Ma'anar al'ada ta haɗu da duk ma'auni, metadata, siffofi, ƙarfin juzu'i, da sauran bayanai a cikin JSON file. Opentrons Flex yana buƙatar wannan bayanin don fahimtar yadda ake aiki da labware na al'ada. Duba sashin Ma'anar Ma'anar Labware na Musamman don ƙarin bayani.
5.3 Faranti mai kyau
Opentrons Flex yana aiki ta tsohuwa tare da faranti da aka jera a ƙasa. Yin amfani da waɗannan faranti rijiyoyin yana taimakawa rage nauyin aikin ku na shirye-shiryen saboda an shirya su ta atomatik daga cikin akwatin. Hakanan ana samun bayanan faranti a cikin ɗakin karatu na Opentrons Labware.

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6-faranti rijiya
Manufacturer Corning

Ƙayyadaddun bayanai
6 rijiyoyi 16.8 ml/ rijiyoyin madauwari, lebur kasa

API load sunan coring_6_wellplate_16.8ml_flat

12-faranti rijiya
Manufacturer Corning

Ƙayyadaddun bayanai
12 rijiyoyi 6.9 ml/ rijiyoyin madauwari, lebur kasa

API load sunan coring_12_wellplate_6.9ml_flat

24-faranti rijiya
Manufacturer Corning

Ƙayyadaddun bayanai
24 rijiyoyi 3.4 ml/ rijiyoyin madauwari, lebur kasa

API load sunan coring_24_wellplate_3.4ml_flat

48-faranti rijiya
Manufacturer Corning

Ƙayyadaddun bayanai
48 rijiyoyi 1.6 ml/ rijiyoyin madauwari, lebur kasa

API load sunan coring_48_wellplate_1.6ml_flat

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96-faranti rijiya
Mai ƙera Bio-Rad Corning NEST
Opentrons Thermo Scientific
Amurka Kimiyya

Ƙayyadaddun bayanai
96 rijiyoyin 200 µL/ rijiyoyin madauwari, V kasa
96 rijiyoyin 360 µL/ rijiyoyin madauwari, lebur ƙasa
96 rijiyoyin 100 µL/ rijiyoyin madauwari, V kasa PCR cikakken siket
96 rijiyoyin 200 µL/ rijiyoyin madauwari, lebur ƙasa
96 zurfin rijiyoyi 2000 µL/ rijiyoyin murabba'i, V kasa
Rijiyoyin tauri 96 200 µL/ rijiyoyin madauwari, V kasa PCR cikakken siket
Nunc 96 zurfin rijiyoyin 1300 µL/ rijiyoyin madauwari, U kasa
Nunc 96 zurfin rijiyoyin 2000 µL/ rijiyoyin madauwari, U kasa
96 zurfin rijiyoyi 2.4 ml/ rijiyoyin murabba'i, U kasa

Sunan nauyin API biorad_96_wellplate_200ul_pcr
corning_96_wellplate_360ul_flat
nest_96_wellplate_100ul_pcr_full_skirt
nest_96_wellplate_200ul_flat
nest_96_wellplate_2ml_deep
opentrons_96_wellplate_200ul_pcr_full_ skirt
thermoscientificnuc_96_wellplate_ 1300ul thermoscientificnuc_96_wellplate_ 2000ul usascientific_96_wellplate_2.4ml_deep

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384-faranti rijiya

Manufacturer Applied Biosystems Bio-Rad
Corning

Ƙayyadaddun bayanai
384 rijiyoyin 40 µL/ rijiyoyin madauwari, V kasa
384 rijiyoyin 50 µL/ rijiyoyin madauwari, V kasa
384 rijiyoyin 112 µL/ rijiyoyin murabba'i, lebur ƙasa

Sunan nauyin API amfani da biosystemsmicroamp_384_ rijiya_40ul biorad_384_wellplate_50ul
corning_384_wellplate_112ul_flat

To farantin adaftan
Faranti na aluminium da aka jera a ƙasa sune adaftan thermal don tsarin Opentrons Heater-Shaker GEN1. Kuna iya amfani da waɗannan ma'anar adaftar da ke tsaye don loda tabbataccen Opentrons ko labware na al'ada a saman Heater-Shaker.

Nau'in adaftar Opentrons Universal Flat Heater-Shaker Adaftan Buɗewa 96 PCR Adaftar Wuta-Shaker Buɗewa 96 Zurfin Rijiyar Wutar Wuta-Shaker Adaftan Buɗewa 96 Flat Bottom Heater-Shaker Adapter

Sunan nauyin API opentrons_universal_flat_adapter opentrons_96_pcr_adapter opentrons_96_deep_well_adapter opentrons_96_flat_bottom_adapter

Hakanan zaka iya loda duka adaftan da labware tare da ma'anar guda ɗaya. Laburaren mu na Labware ya haɗa da adaftar zafi da aka riga aka tsara da yawa da haɗaɗɗun labware waɗanda ke sa Heater-Shaker ya shirya don amfani da shi kai tsaye daga cikin akwatin.

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Lura: Kada a yi amfani da ma'anar da aka haɗa idan kuna buƙatar matsar da labware akan ko kashe na Heater-Shaker yayin ƙa'idar ku, ko dai tare da gripper ko da hannu. Yi amfani da ma'anar adaftar da ke tsaye maimakon.

Haɗin Adafta/Labware

API load name

Opentrons 96 Deep Rijiyar Wuta-Shaker Adafta tare da NEST Deep Rijiyar Plate 2 mL

opentrons_96_deep_well_adapter_nest_ wellplate_2ml_deep

Opentrons 96 Flat Bottom Heater-Shaker Adafta tare da NEST 96 Well Plate 200 µL Flat

opentrons_96_flat_bottom_adapter_nest_ wellplate_200ul_flat

Opentrons 96 PCR Adaftar Heater-Shaker tare da NEST Well Plate 100 µL

opentrons_96_pcr_adapter_nest_wellplate_ 100ul_pcr_full_skirt

Opentrons Universal Flat Heater-Shaker Adafta tare da Corning 384 Well Plate 112 µL Flat

opentrons_universal_flat_adapter_corning_384_ rijiyar_112ul_flat

Ana iya siyan adaftar kai tsaye daga Opentrons a https://shop.opentrons.com.

To faranti da API ma'anar
Laburaren Laburaren Labware na Opentrons yana bayyana halayen faranti da aka jera a sama a JSON daban files. Robot Flex da Opentrons Python API sun dogara da waɗannan ma'anar JSON don aiki tare da labware da ka'idojin ku ke amfani da su. Domin misaliampHar ila yau, lokacin aiki tare da API, aikin ProtocolContext.load_labware yana karɓar waɗannan sunayen labware azaman ingantattun sigogi a lambar ku. Sunayen lodin API masu alaƙa suna haɗawa da ma'anar ma'anar farantin rijiyar a cikin ma'ajin Opentrons GitHub.

Custom rijiyar faranti labware
Gwada amfani da Opentrons Labware Creator don yin ma'anar labware na al'ada idan ba a jera farantin rijiyar da kuke son amfani da ita anan ba. Ma'anar al'ada ta haɗu da duk ma'auni, metadata, siffofi, ƙarfin juzu'i, da sauran bayanai a cikin JSON file. Opentrons Flex yana karanta wannan bayanin don fahimtar yadda ake aiki da labware na al'ada. Duba sashin Ma'anar Ma'anar Labware don ƙarin bayani.

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5.4 Nasihu da tukwici
Opentrons Flex tukwici sun zo cikin 50 µL, 200 µL, da 1000 µL masu girma dabam. Waɗannan fayyace ne, nasihun polypropylene maras gudanarwa waɗanda ke samuwa tare da ko ba tare da tacewa ba. An tattara su bakararre a cikin akwatunan da ke riƙe tukwici 96 kuma ba su da DNase, RNase, protease, pyrogens, DNA ɗan adam, endotoxins, da masu hana PCR. Racks kuma sun haɗa da lambobin kuri'a da kwanakin ƙarewa.
Tukwici na Flex pipette suna aiki tare da Opentrons Flex 50 µL da 1000 µL pipettes a cikin saitunan 1-, 8-, da 96-tashar. Yayin da zaku iya sanya kowane nasihu na Flex akan pipettes 50 L da 1000 L, kuyi ƙoƙarin daidaita tip zuwa ƙimar ƙimar pipette. Domin misaliampHar ila yau, yana iya zama m don saka tip 1000 L akan pipette 50 L. Don 1000 L pipette, tabbas za ku iya amfani da tip 50 L, 200 L, ko 1000 L.

Tukwici
Abubuwan da ba a tace su ba kuma an haɗa su a cikin tarkace wanda ya ƙunshi farantin tushe da za a sake amfani da shi, farantin tsakiyar da ke ɗauke da tukwici 96, da murfi.

Tukwici ta ƙara 50 µL 200 µL 1000 µL

API load name
Ba a tace: opentrons_flex_96_tiprack_50ul Tace: opentrons_flex_96_filtertiprack_50ul
Ba a tace: opentrons_flex_96_tiprack_200ul Tace: opentrons_flex_96_filtertiprack_200ul
Ba a tace: opentrons_flex_96_tiprack_1000ul Tace: opentrons_flex_96_filtertiprack_1000ul

Don taimakawa tare da ganowa, faranti na tip na tsakiyar faranti suna da lambobi masu launi bisa girman tip:
50 µL: magenta 200 L: rawaya 1000 µL: blue

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Lokacin yin oda ko sake yin oda, tukwici da racks suna zuwa cikin tsari guda biyu daban daban:
Racks: Ya ƙunshi raƙuman tip ɗin da aka nannade daban-daban (farantin tushe, farantin tsakiyar tare da tukwici, da murfi). Saitunan da aka ɗora sun fi kyau lokacin da tsabta ta kasance mafi mahimmanci, don guje wa gurɓatawa, ko lokacin da ka'idojin ku ba su ba da izinin sake amfani da farantin tushe ko kayan aiki ba.
Sake cikawa: Ya ƙunshi cikakken rakiyar tukwici guda ɗaya (farantin gindi, farantin tsakiya tare da tukwici, da murfi) da kwantena ɗaya tip. Matsakaicin cikawa ya fi kyau lokacin da ka'idojin ku suka ba da izinin sake amfani da farantin tushe ko kayan aiki.
Daidaituwar Tippipet
Flex pipette tukwici an tsara su don Opentrons Flex pipettes. Tukwici masu sauƙi ba su dace da baya ba tare da Opentrons OT-2 pipettes, kuma ba za ku iya amfani da shawarwarin OT-2 akan Flex pipettes ba.
Wasu nasihu masu ma'ana na masana'antu na iya aiki tare da Flex pipettes, amma wannan ba a ba da shawarar ba. Don tabbatar da ingantaccen aiki, yakamata ku yi amfani da tukwici na Opentrons Flex kawai tare da Flex pipettes.
Adaftar tukwici
Pipette mai tashar tashoshi 96 yana buƙatar adaftar don haɗa cikakken tarin tukwici yadda yakamata. A lokacin aikin haɗin gwiwa, pipette yana motsawa a kan adaftan, ya sauke kansa a kan fil ɗin hawa, kuma yana jan tukwici a kan pipettes ta ɗaga adaftan da tip.

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Lura: Yi amfani da adaftar tip ɗin kawai lokacin ɗaukar cikakken tukwici a lokaci ɗaya. Sanya raƙuman tukwici kai tsaye a kan bene lokacin ɗaukar ƴan tukwici.
Gargaɗi: Haɗarin tsinke. Tsare hannaye daga adaftan tip yayin da pipette ke haɗa tukwici na pipette.

Nau'in adaftar Opentrons Flex 96 Tukwici Rack Adafta

Sunan nauyin API opentrons_flex_96_tiprack_adapter

Adaftar tip rack ya dace da Opentrons Flex Gripper. Kuna iya amfani da gripper don sanya sabbin ramukan tukwici akan adaftar ko don ɗauka da matsar da raƙuman titin da aka yi amfani da su zuwa cikin sharar gida.

5.5 tubes da tubs

Tsarin Opentrons 4-in-1 Tube Rack yana aiki tare da Opentrons Flex ta tsohuwa. Yin amfani da tarakin bututun 4-in-1 yana taimakawa rage nauyin aikin ku na shirye-shiryen saboda haɗin gwiwar da yake bayarwa suna shirye-shiryen sarrafa kansa kai tsaye daga cikin akwatin. Ana kuma samun ƙarin bayani a cikin Laburaren Laburaren Labware na Opentrons.

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Haɗin Tube da tara
Bututun bututu na Opentrons 4-in-1 yana goyan bayan nau'ikan nau'ikan bututu iri-iri, guda ɗaya ko cikin nau'ikan girman (girma) haɗuwa. Waɗannan sun haɗa da: 6-tube tara don bututun 50 ml (6 x 50 ml). 10-tube hade tara ga hudu 50 ml tubes da shida 15 ml shambura (4 x 50 ml, 6 x 15 ml). 15-tube tara don 15 ml tubes (15 x 15 ml). 24-tube tara don 0.5 ml, 1.5 ml, ko 2 ml bututu (24 x 0.5 ml, 1.5 ml, 2 ml).
Lura: Duk bututun silindi ne tare da gindin V-dimbin yawa (conical) sai dai in an nuna.

6-rubutun tube
Nau'in tube 6 Falcon 50 ml 6 NEST 50 ml
10-rubutun tube
Nau'in tube 4 Falcon 50 ml 6 Falcon 15 ml 4 NEST 50 ml 6 NEST 15 ml

Sunan nauyin API opentrons_6_tuberak_falcon_50ml_conical opentrons_6_tuberack_nest_50ml_conical
API load name opentrons_10_tuberack_falcon_4x50ml_6x15ml_conical opentrons_10_tuberack_nest_4x50ml_6x15ml_conical

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15-rubutun tube
Nau'in tube 15 Falcon 15 ml 15 NEST 15 ml

Sunan nauyin API opentrons_15_tuberak_falcon_15ml_conical opentrons_15_tuberack_nest_15ml_conical

24-rubutun tube

Nau'in tube
24 Eppendorf Safe-Lock 1.5 ml 24 Eppendorf Safe-Lock 2 ml, U-dimbin ƙasa 24 generic 2 ml dunƙule hula 24 NEST 0.5 ml dunƙule hula 24 NEST 1.5 mL dunƙule hula 24 NEST 1.5 m24 screw cap 2 mL NEST hula 24 mL NEST 2 mL hular ɗaukar hoto, ƙasa mai siffar U

Sunan nauyin API opentrons_24_tuberack_eppendorf_1.5ml_safelock_snapcap opentrons_24_tuberak_ependorf_2ml_safelock_snapcap
opentrons_24_tuberack_generic_2ml_screwcap opentrons_24_tuberack_nest_0.5ml_screwcap opentrons_24_tuberack_nest_1.5ml_screwcap opentrons_24_tuberack_nest_1.5ml_snapcap opentrons_24_tuberack_nest_2ml_screwcap opentrons_24_tuberack_nest_2ml_snapcap

Ma'anar Tube rack API
Laburaren Laburaren Labware na Opentrons yana bayyana halayen raƙuman bututun da aka jera a sama a cikin JSON daban files. Robot Flex da Opentrons Python API sun dogara da waɗannan ma'anar JSON don aiki tare da labware da ka'idojin ku ke amfani da su. Domin misaliampHar ila yau, lokacin aiki tare da API, aikin ProtocolContext.load_labware yana karɓar waɗannan sunayen labware azaman ingantattun sigogi a lambar ku. Sunaye masu nauyin API masu alaƙa suna haɗawa da ma'anar labware na bututu a cikin ma'ajin Opentrons GitHub.

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Custom tube tara labware
Gwada ƙirƙirar ma'anar labware na al'ada ta amfani da Opentrons Labware Creator idan ba'a jera bututu da haɗin rak ɗin da kuke son amfani da shi anan ba. Ma'anar al'ada ta haɗu da duk ma'auni, metadata, siffofi, ƙarfin juzu'i, da sauran bayanai a cikin JSON file. Opentrons Flex yana karanta wannan bayanin don fahimtar yadda ake aiki da labware na al'ada. Duba sashin Ma'anar Ma'anar Labware na Musamman don ƙarin bayani.
5.6 Aluminum tubalan
Aluminum tubalan jirgi tare da Module Temperatuur GEN2 kuma ana iya siyan shi daban azaman saitin yanki uku. Saitin ya haɗa da farantin ƙasa mai lebur, toshe mai rijiyoyi 24, da shingen rijiyoyi 96.
Opentrons Flex yana amfani da tubalan aluminum don riƙe sample tubes da rijiyoyin faranti akan Module Zazzabi ko kai tsaye akan bene. Lokacin amfani da Module na Zazzabi, tubalan aluminium na iya kiyaye sample tubes, PCR tubes, ko faranti a akai-akai zazzabi tsakanin 4 °C da 95 °C.
Flat kasa farantin
Farantin ƙasa mai lebur don jiragen ruwa na Flex tare da ɗimbin zafin jiki na Module kuma yana dacewa da daidaitattun faranti na ANSI/SLAS daban-daban. Wannan farantin lebur ya bambanta da farantin da ke jigilar da Temperature Module kanta ko saitin guda uku daban daban. Yana fasalta faffadan aiki mai faɗi da shirye-shiryen bidiyo na kusurwa. Waɗannan fasalulluka suna taimakawa haɓaka aikin Opentrons Flex Gripper lokacin motsi labware akan ko kashe farantin.
Kuna iya faɗi wane farantin ƙasa mai lebur ɗin da kuke da shi saboda na Flex yana da kalmomin "Opentrons Flex" a saman samansa. Nau'in na OT-2 bai yi ba.

OPENTRONS FLEX

BABI NA 5: LABWARE

24-da kyau aluminum block
Ana amfani da toshe rijiyoyin 24 tare da kowane sample vials. Don misaliample, yana karban sampda wadannan abubuwa:
Yi kasa mai siffar V ko U-dimbin yawa. Amintaccen abun ciki tare da hular karye ko rufe hula. Riƙe ruwa a cikin ƙarfin 0.5 ml, 1.5 ml, da 2 ml.

96-da kyau aluminum block
Katangar rijiyar 96 tana goyan bayan nau'ikan faranti iri-iri. Don misaliample, yana karɓar faranti masu kyau waɗanda sune:
Daga manyan masana'antun faranti kamar Bio-Rad da NEST.
An ƙera shi da ƙasa mai siffar V, gindin U-dimbin yawa, ko ƙasa lebur.
An tsara shi da rijiyoyin 100 µL ko 200 µL.
Hakanan yana dacewa da jigon PCR na gabaɗaya.

Adaftan tsaye
Thermal block Flex lebur kasa farantin 24-riji aluminum toshe 96-riji aluminum block

Sunan kaya na API opentrons_aluminum_flat_bottom_plate Dubi hada-hadar labware a kasa. opentrons_96_well_aluminum_block

Aluminum block labware haduwa
Laburaren Laburaren Labware na Opentrons yana goyan bayan toshe mai zuwa, vial, da haɗe-haɗen faranti, waɗanda kuma an ayyana su cikin ma'anar labware na JSON daban. files. Robot Flex da Opentrons Python API

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BABI NA 5: LABWARE
dogara ga waɗannan ma'anar JSON don aiki tare da labware da ka'idojin ku ke amfani da su. Don misaliampHar ila yau, lokacin aiki tare da API, aikin ProtocolContext.load_labware yana karɓar waɗannan sunayen labware azaman ingantattun sigogi a lambar ku. Teburan da ke ƙasa suna lissafin tsoffin haɗin toshe/kwantena da sunayen lodin API masu alaƙa. Hanyoyin haɗi suna haɗi zuwa ma'anar JSON masu dacewa a cikin ma'ajin Opentrons GitHub.
Lura: Duk bututu suna da gindin V-dimbin yawa sai dai in an nuna su.

24-da kyau aluminum block labware haduwa

24-riji toshe abun ciki Generic 2 mL dunƙule hula NEST 0.5 mL dunƙule hula NEST 1.5 mL dunƙule hula NEST 1.5 mL karye hula NEST 2 mL dunƙule hula NEST 2 mL hula hula, U-dimbin yawa kasa

API load name opentrons_24_aluminumblock_generic_2ml_screwcap opentrons_24_aluminumblock_nest_0.5ml_screwcap opentrons_24_aluminumblock_nest_1.5ml_screwcap opentrons_24_aluminumblock_nest_p1.5ml crewcap opentrons_24_aluminumblock_nest_2ml_snapcap

96-da kyau aluminum block labware haduwa

96-riji toshe abun ciki Bio-Rad rijiyar farantin 200 µL Generic PCR tsiri 200 µL NEST rijiyar farantin 100 µL

API load name opentrons_96_aluminumblock_biorad_wellplate_200uL opentrons_96_aluminumblock_generic_pcr_strip_200uL opentrons_96_aluminumblock_nest_wellplate_100uL

OPENTRONS FLEX

BABI NA 5: LABWARE

5.7 Labware da Opentrons Flex Gripper
Kodayake Opentrons Flex yana aiki tare da duk kaya a cikin Laburaren Labware, Opentrons Flex Gripper ya dace da takamaiman abubuwan labware kawai. A halin yanzu, an inganta Gripper don amfani tare da abubuwan labware masu zuwa.

Rukunin Labware Mai Zurfafa Rijiyar Faranti Cikakkiyar Skirted 96 Rijiyoyin Rijiyoyi
Tip Racks (ba a tace ba kuma ba a tace ba)

Alamomi
NEST 96 Zurfin Rijiyar Plate 2 ml
Opentrons Tauri 96 Rijiyar Plate 200 µL PCR Cikakken Skirt NEST 96 Rijiyar Farantin 200 µL Flat
Opentrons Flex 96 Tukwici Rack 50 µL Buɗewar Tukwici Flex 96 Tukwici Rack 200 µL Opentrons Flex 96 Tip Rack 1000 µL

Lura: Don kyakkyawan sakamako, yi amfani da Flex Gripper kawai tare da labware da aka jera a sama. Flex Gripper na iya aiki tare da sauran kayan aikin ANSI/SLAS masu dacewa da kayan aiki, amma wannan ba a ba da shawarar ba.

5.8 Ma'anonin labware na al'ada
Kamar yadda aka tattauna a farkon wannan babin, labware na al'ada labware ne waɗanda ba a jera su a cikin Laburaren Laburaren Labura na Opentrons ba. Kuna iya amfani da wasu na gama-gari ko na musamman na labware tare da Flex ta hanyar auna daidai da yin rikodin halayen abin da adana bayanan a cikin JSON file. Lokacin da aka shigo da shi cikin ƙa'idar, Flex da API suna amfani da wannan bayanan JSON don yin hulɗa tare da labware ɗin ku. Opentrons yana ba da kayan aiki da ayyuka, waɗanda za mu bincika a ƙasa, don taimaka muku amfani da Flex tare da labware na al'ada.

OPENTRONS FLEX

BABI NA 5: LABWARE

Ƙirƙirar ma'anar labware na al'ada
Kayan aikin Opentrons da sabis suna taimakawa sanya labware na al'ada cikin isar ku. Waɗannan fasalulluka suna ɗaukar matakan fasaha daban-daban da hanyoyin aiki. Ƙirƙirar labware na kanku, da amfani da shi tare da Opentrons Flex, yana taimakawa wajen sa mutum-mutumi ya zama mai juzu'i da ƙarfi ga ɗakin binciken ku.

CUSTOM LABWARE MAHALICI
Mai ƙirƙira Labware na Custom ba-ladi ba ne, web- kayan aiki mai tushe wanda ke amfani da ƙirar hoto don taimaka muku ƙirƙirar ma'anar labware file. Mahaliccin Labware yana samar da ma'anar labware na JSON file da kuke shigo da su cikin Opentrons App. Bayan haka, kayan aikin ku na al'ada yana samuwa ga Flex robot da Python API.

HIDIMAR LABWARE CUSTOM
Tuntuɓi mu idan labware ɗin da kuke son amfani da shi ba ya samuwa a cikin ɗakin karatu, idan ba za ku iya ƙirƙirar ma'anar ku ba, ko saboda wani abu na al'ada ya ƙunshi siffofi daban-daban, girma, ko wasu rashin daidaituwa da aka bayyana a ƙasa.

Labware zaka iya ayyana a cikin Mahaliccin Labware
; Rijiyoyi da bututun iri ɗaya ne kuma iri ɗaya ne. ; Duk layuka suna da wuri daidai gwargwado
( sarari tsakanin layuka daidai yake).
; Duk ginshiƙai suna da wuri daidai gwargwado (sarari tsakanin ginshiƙai daidai yake).
; Yayi daidai da kyau a cikin rami ɗaya.

Labware Opentrons yana buƙatar ayyana; Rijiyoyi da siffofin bututu sun bambanta. ; Ba a daidaita layuka daidai gwargwado.
; ginshiƙai ba su daidaita ba.
; Karami fiye da ɗaya ramin bene (yana buƙatar adaftar) ko ya faɗi ramummuka da yawa.

OPENTRONS FLEX

BABI NA 5: LABWARE
Anan akwai wasu zane-zane da ke taimaka muku hango tsohonampkamar yadda aka bayyana a sama. Na yau da kullun Duk ginshiƙai suna da wuri daidai gwargwado kuma duk layuka suna jeri daidai gwargwado. ginshiƙai baya buƙatar samun tazara iri ɗaya da layuka.
Na yau da kullun ba dole ba ne grid ya kasance a tsakiyar labware.
Layukan da ba bisa ka'ida ba suna da wuri daidai gwargwado amma ginshiƙai ba su daidaita ba.
Layukan da ba su bi ka'ida ba suna da wuri daidai gwargwado amma rijiyoyin ba iri ɗaya ba ne.
Ba bisa ka'ida ba Akwai grid fiye da ɗaya.
Ƙungiyar mu na labware za ta yi aiki don fahimtar bukatunku da ƙirƙira ma'anar labware na al'ada a gare ku. Dubi labaran goyan bayan Neman Ma'anar Labware na Musamman da Form ɗin Buƙatar Labware na Musamman don ƙarin bayani. Wannan sabis ne na tushen kuɗi.

OPENTRONS FLEX

BABI NA 5: LABWARE

API ɗin Python
Duk da yake ba za ku iya ƙirƙirar labware na al'ada tare da API ɗinmu ba, kuna iya amfani da labware na al'ada tare da hanyoyin API ɗin da ke akwai. Koyaya, kuna buƙatar fara ayyana labware na al'ada da shigo da shi cikin Opentrons App. Da zarar kun ƙara labware ɗin ku zuwa Opentrons App, yana samuwa ga Python API da robot. Duba sashin Ma'anar Ma'anar Labware na Musamman na takaddun API na Python don ƙarin bayani. Don bayani game da rubuta rubutun ladabi tare da API, duba sashin API ɗin Protocol Protocol a cikin babin Haɓaka yarjejeniya.
JSON labware tsarin
A JSON file shine tsarin ƙirar Opentrons da labware na al'ada. Wannan file ya ƙunshi kuma yana tsara bayanan labware bisa ga ƙayyadaddun ƙira da aka saita ta tsarin tsoho.
Tsari shine firam

Takardu / Albarkatu

budetrons FLEX FLEX Opentrons Flex Buɗe Tushen Liquid Handling Robot [pdf] Jagoran Jagora
FLEX Opentrons Flex Buɗe tushen Ruwan Robot, FLEX, Opentrons Flex Buɗe Tushen Liquid Handling Robot, Flex Buɗe Tushen Liquid Robot, Buɗe Tushen Liquid Robot, Tushen Ruwan Robot, Robot Mai Kula da Liquid, Robot Sarrafa, Robot

Magana

Manual mai amfani

FLEX Opentrons Flex Buɗe Tushen Liquid Handling Robot

Opentrons Flex

Ƙayyadaddun bayanai:

Umarnin Amfani da samfur:

1. Shigarwa da Daidaita Kayan aiki:

2. Matsar:

3. Haɗi:

4. Mai tsara yarjejeniya:

5. Python Protocol API:

6. OT-2 ladabi:

Tambayoyin da ake yawan yi (FAQ):

Tambaya: Ta yaya zan magance matsalar idan robot baya motsi kamar
sa ran?

Tambaya: Zan iya amfani da pipettes na al'ada tare da Opentrons Flex?

Takardu / Albarkatu

Magana

Bar sharhi

Soke amsa

FLEX Opentrons Flex Buɗe Tushen Liquid Handling Robot

Opentrons Flex

Ƙayyadaddun bayanai:

Umarnin Amfani da samfur:

1. Shigarwa da Daidaita Kayan aiki:

2. Matsar:

3. Haɗi:

4. Mai tsara yarjejeniya:

5. Python Protocol API:

6. OT-2 ladabi:

Tambayoyin da ake yawan yi (FAQ):

Tambaya: Ta yaya zan magance matsalar idan robot baya motsi kamar sa ran?

Tambaya: Zan iya amfani da pipettes na al'ada tare da Opentrons Flex?

Takardu / Albarkatu

Magana

Labarai masu alaka

Bar sharhi

Soke amsa

Tambaya: Ta yaya zan magance matsalar idan robot baya motsi kamar
sa ran?