Litlhaloso Kakaretso: Lorem ipsum dolor sit
amet, consectetur adipiscing elit. Sed etsa eiusmod tempor incididunt
ut labore et dolore magna aliqua.
Litlhaloso tsa Tikoloho: Lorem ipsum
dolor sit amet, consectetur adipiscing elit. Sed etsa eiusmod tempor
incididunt ut labore et dolore magna aliqua.

Setifikeiti: Lorem ipsum dolor sit amet,
consectetur adipiscing elit. Sed do eiusmod tempor incididunt ut
labore et dolore magna aliqua.
Nomoro ea letoto: XXX-XXXX-XXXX

Litaelo tsa Tšebeliso ea Sehlahisoa:

1. Kemiso ea lisebelisoa le ho lekanya:

Latela mehato e hlalositsoeng bukeng ea pipette le gripper
ho kenya.

2. Phalliso:

Bakeng sa mehato e khutšoanyane, sheba karolo ea 2.5 bukeng ea litaelo. Bakeng sa
maeto a malelele, latela litataiso tse fanoeng. Kakaretso e tsamayang
keletso e teng hape.

3. Lihokelo:

Netefatsa hore ho na le khokahanyo e nepahetseng ea motlakase joalokaha ho hlalositsoe bukeng ea tataiso.
Hokela USB le lisebelisoa tse thusang ha ho hlokahala. Likhokahano tsa marang-rang
e lokela ho thehoa ho latela litaelo.

4. Moqapi oa Protocol:

Utloisisa litlhoko tsa Protocol Designer 'me u ithute ho
ho rala liprothokholo tse ncha kapa ho fetola tse seng li ntse li le teng ho latela lab ea hau
ditlhoko.

5. Python Protocol API:

Lekola ho ngola le ho tsamaisa lingoliloeng u sebelisa Python Protocol API.
Fumana likarolo tse ikhethileng tsa Python bakeng sa ts'ebetso e ntlafalitsoeng.

6. OT-2 Protocols:

Ithute ka OT-2 Python protocols, OT-2 JSON protocols, le
Liprothokholo tsa Magnetic Module tsa mefuta e fapaneng ea liteko.

Lipotso Tse Botsoang Khafetsa (FAQ):

P: Nka rarolla bothata joang haeba roboto e sa tsamaee joalo ka
lebeletsoe?

A: Sheba khokahanyo ea matla, etsa bonnete ba hore ho na le tekano e nepahetseng ea
lisebelisoa, le ho netefatsa hore ha ho na litšitiso ho
tsela ea roboto.

P: A na nka sebelisa li-pipette tse tloaelehileng ka Opentrons Flex?

A: Opentrons e khothaletsa ho sebelisa li-pipette tse lumellanang hantle
tshebetso le nepahalo.

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Opentrons Flex
Buka ea Tataiso
Litlhaloso tsa likarolo tsa Opentrons Labworks Inc.
Tšitoe 2023

© OPENTRONS 2023 Opentrons FlexTM (Opentrons Labworks, Inc.) Mabitso a ngodisitsweng, matshwao a kgwebo, jj. a sebelisitsoeng tokomaneng ena, le ha a sa tshwauwa ka ho kgetheha jwalo, ha a lokelwa ho nkuwa a sa sireletsoa ke molao.

Tafole ea likateng
Selelekela . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 Sebopeho sa bukana ena . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Lintlha le litemoso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Khaolo ea 1: Selelekela . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 1.1 Rea u amohela ho Opentrons Flex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Ke eng e ncha ho Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Lits'ebetso tsa Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 1.2 Litaba tsa polokeho . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Matšoao a tšireletso. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Litemoso tsa polokeho ea motlakase . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Litemoso tse ling tsa polokeho . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 Litemoso tsa polokeho . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 Tšireletseho ea likokoana-hloko . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Mosi o chefo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Mekelikeli e tukang. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 1.3 Ho latela melao ea tsamaiso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Tšireletseho . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Tšebelisano ea motlakase . . . . . . . . . . . . . . . . . . . . . . . . .20 Litemoso le lintlha tsa FCC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Ho latela melao ea ISED ea Canada . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Temoso ea tikoloho . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 netefatso ea Wi-Fi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Khaolo ea 2: Ho kenya le ho Fallisoa . . . . . . . . . . . . . . . . . . . . . . . 22 2.1 Litlhoko tsa polokeho le ts'ebetso. . . . . . . . . . . . . . . . . . . . .22 Moo u ka behang Opentrons Flex . . . . . . . . . . . . . . . . . . . . . . . .22 Tšebeliso ea matla . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 Maemo a tikoloho . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 2.2 Unboxing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Boiteko le nako e hlokahalang . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Kereiti le thepa ea ho paka . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Lintlha tsa sehlahisoa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 Karolo ea 1: Tlosa kreiti . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27 Karolo ea 2: Lokolla Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29 Karolo ea 3: Kopano ea ho qetela le matla ho. . . . . . . . . . . . . . . . . . .32

2.3 Lebelo la pele . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Matla ho . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Hokela ho netweke kapa khomphuta . . . . . . . . . . . . . . . . . . . . .36 Kenya liapdeite tsa software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37 Hokela Pendant ea Emergency Stop . . . . . . . . . . . . . . . . . . . . . . 38 Fana ka roboto ea hau lebitso. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
2.4 Ho kenya lisebelisoa le ho lekanya . . . . . . . . . . . . . . . . . 38 Ho kenya Pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 Ho kenya Gripper. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
2.5 Ho falla . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 Mehato e kgutshwane. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Mehato ea sebaka se selelele . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Keletso e akaretsang e susumetsang . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 Mehopolo ea ho qetela mabapi le ho falla. . . . . . . . . . . . . . . . . . . . . . . . . . .44
Khaolo ea 3: Tlhaloso ea Tsamaiso. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 3.1 Likaroloana tsa 'mele . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45 Foreimi le kampeng . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 Teko le sebaka sa ho sebetsa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 Staging sebaka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47 Lisebelisoa tsa mekato. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 Letlapa la litšila. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49 Staglibaka tsa slots . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49 Tsamaiso ea tsamaiso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 Sekirini sa ho tšoara le lisebelisoa tsa LED . . . . . . . . . . . . . . . . . . . . . . . . .51 3.2 Pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 Litlhaloso tsa Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Tekanyetso ea Pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Pipette tip rack adaptara. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Ho nka malebela a sa fellang. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 li-sensors tsa Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 Lintlafatso tsa firmware ea Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 3.3 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 Litlhaloso tsa Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Gripper calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Lintlafatso tsa firmware ea Gripper. . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 3.4 Pendanta ea Emergency Stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 Nako ea ho sebelisa E-stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 Ho kenya letsoho le ho lokolla E-stop . . . . . . . . . . . . . . . . . . . . .60

3.5 Lihokelo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 Khokelo ea matla . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 USB le likhokahano tse thusang. . . . . . . . . . . . . . . . . . . . . . . . .62 Lihokelo tsa marang-rang . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
3.6 Litlhaloso tsa tsamaiso. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63 Litlhaloso tse akaretsang . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63 Litlhaloso tsa tikoloho . . . . . . . . . . . . . . . . . . . . . . . . . .64 Litifikeiti. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 Nomoro ea serial. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64
Khaolo ea 4: Li-module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 4.1 Dimojule tse tsheheditsweng . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66 4.2 Module caddy system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 4.3 Tekanyetso ya mojule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68 Nako ea ho lekanya li-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 Mokhoa oa ho lekanya li-module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 4.4 Heater-Shaker Mojule GEN1 . . . . . . . . . . . . . . . . . . . . . . . . . .70 Likarolo tsa Heater-Shaker. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70 Litlhaloso tsa Heater-Shaker . . . . . . . . . . . . . . . . . . . . . . . . . .72 4.5 Thibelo ea Magnetic GEN1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 Likarolo tsa Magnetic Block. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 Litlhaloso tsa Magnetic Block. . . . . . . . . . . . . . . . . . . . . . . . .74 4.6 Mojule oa Mocheso GEN2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .75 Likarolo tsa Mojule oa Mocheso . . . . . . . . . . . . . . . . . . . . . . . . .75 Litlhaloso tsa Mojule oa Mocheso . . . . . . . . . . . . . . . . . . . .77 4.7 Thermocycler Mojule GEN2. . . . . . . . . . . . . . . . . . . . . . . . . . . .77 Likarolo tsa Thermocycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .78 Litlhaloso tsa Thermocycler . . . . . . . . . . . . . . . . . . . . . . . . . . .79
Khaolo ea 5: Labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 5.1 Likhopolo tsa Labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Labware e le hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Lab e le data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Labure ea tloaelo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81 5.2 Matamo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Matamo a seliba se le seng . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Matamo a liliba tse ngata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Reservoirs le litlhaloso tsa API . . . . . . . . . . . . . . . . . . . . . . . . 83 Custom reservoir labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 5.3 Lipoleiti tsa seliba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

Lipoleiti tse 6 tsa seliba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . lipoleiti tse 84 12 tsa seliba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . lipoleiti tse 84 24 tsa seliba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Lipoleiti tse 84 48-liliba. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . lipoleiti tse 84 96 tsa seliba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . lipoleiti tse 85 384 tsa seliba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 Li-adapter tsa lipoleiti tsa Well plate. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 Lipoleiti tsa Well plate le litlhaloso tsa API. . . . . . . . . . . . . . . . . . . . . . . . .87 Tloaelo hantle poleiti labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 5.4 Malebela le liraka tsa malebela . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 Liraka tsa malebela . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 Tšebelisano ea Tippipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 Adapter ea rack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 5.5 Li-tubes le li-tube racks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90 Metsoako ea li-tube le rack. . . . . . . . . . . . . . . . . . . . . . . . . . . 91 6-tube liraka. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 10-tube liraka. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 15-tube liraka. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 24-tube liraka. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Litlhaloso tsa Tube rack API . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Custom tube rack labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 5.6 Diboloko tsa aluminium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 Letlapa le tlase le bataletseng. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 boloko ba aluminium ea liliba tse 24. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 boloko ba aluminium ea liliba tse 96. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Li-adapter tse ikemetseng . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Aluminium block labware metsoako . . . . . . . . . . . . . . . .94 24-libabole tsa aluminium block labware metsoako . . . . . . . . .95 96-libabole tsa aluminium block labware metsoako . . . . . . . . .95 5.7 Labware le Opentrons Flex Gripper . . . . . . . . . . . . . . .96 5.8 Litlhaloso tse tloahelehileng tsa labware . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 Ho theha litlhaloso tsa "laborare" tse ikhethileng . . . . . . . . . . . . . . . . . . .97 JSON labware schema . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99 Litlhaloso tsa labware tsa JSON . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104 Khaolo ea 6: Ntlafatso ea Tsamaiso . . . . . . . . . . . . . . . . . . . . . . . . . . .105 6.1 Mekhoa e entsoeng esale pele . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105 Laeborari ea Tsamaiso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105 Tšebeletso ea Custom Protocol Development . . . . . . . . . . . . . . . .

6.2 Moqapi oa Protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109 Litlhokahalo tsa Moqapi oa Protocol. . . . . . . . . . . . . . . . . . . . . . 109 Ho theha protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Ho fetola mekhoa e teng. . . . . . . . . . . . . . . . . . . . . . . . . . 114
6.3 Python Protocol API . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 Ho ngola le ho tsamaisa mengolo . . . . . . . . . . . . . . . . . . . . . . . . . . . Likarolo tse ikhethileng tsa 115 Python. . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
6.4 OT-2 protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117 OT-2 Python protocols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118 OT-2 JSON protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120 Magnetic Module protocols . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Khaolo ea 7: Software le Ts'ebetso. . . . . . . . . . . . . . . . . . . . . . . . . . 121 7.1 Ts'ebetso ea sekirini sa ho ama. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 Dashboard ea liroboto. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 Tsamaiso ea tsamaiso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122 Lintlha tsa Protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124 Matha ho seta. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127 Labware Position Check. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128 Matha tsoelopele. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130 Ho qeta ho matha . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131 Tsamaiso ea liletsa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132 Litlhophiso tsa roboto. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133 Tlhophiso ea Deck. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136 7.2 Opentrons App. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 Ho kenya app. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 Ho fetisetsa liprothokholo ho Flex. . . . . . . . . . . . . . . . . . . . . . . . . 140 Boemo le ditaolo tsa mojule . . . . . . . . . . . . . . . . . . . . . . . . . . . 142 Protocol ea morao-rao e sebetsa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143 7.3 Ts'ebetso e tsoetseng pele . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144 Jupyter Notebook . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144 Ts'ebetso ea mola oa taelo holim'a SSH. . . . . . . . . . . . . . . . . . . . 145
Khaolo ea 8: Tlhokomelo le Tšebeletso . . . . . . . . . . . . . . . . . . . . . . . .147 8.1 Ho hloekisa Flex ea hau . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Pele o qala . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Seo u ka se hloekisang . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Litharollo tsa ho hloekisa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148 Ho hloekisa foreimi le liphanele tsa lifensetere . . . . . . . . . . . . . . . . . . . . 148 Ho hloekisa matekoane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148

Ho hloekisa gantry. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 Ho hloekisa litšepe tsa litšila. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 8.2 Ho hloekisa lipeipi le malebela . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 Ho hloekisoa ha Pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150 Malebela a ho hloekisa pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151 8.3 Ho hloekisa gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152 8.4 Limojule tsa ho hloekisa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 Ho hloekisa mojule ka kakaretso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 Thermocycler litiiso. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 8.5 Autoclave-safe labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 8.6 Servicing Flex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 Litšebeletso tsa Opentrons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 Tlhophiso ea ho kenya le ts'ebetso ea ts'ebetso. . . 155 Tlhokomelo e thibelang . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156 Tiisetso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157 Sehlomathiso A: Bukana ea ho hlalosa mantsoe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .158 Sehlomathiso B: Litokomane Tse Eketsehileng . . . . . . . . . . . . . . . . . . . . . .170 B.1 Opentrons Knowledge Hub . . . . . . . . . . . . . . . . . . . . . . . . . . . . 170 B.2 Litokomane tsa Python Protocol API. . . . . . . . . . . . . . . . . . . 170 B.3 Opentrons HTTP API reference. . . . . . . . . . . . . . . . . . . . . . . . 171 B.4 Litokomane tsa Moqapi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171 Sehlomathiso C: Software ea Open-Source . . . . . . . . . . . . . . . . . . . . . . . . .172 C.1 Opentrons ho GitHub. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172 C.2 Opentrons monorepo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173 C.3 Libaka tse ling tsa polokelo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175 Sehlomathiso D: Tšehetso le Boitsebiso ba Boikopanyo . . . . . . . . . . . . . . .176 D.1 Lithekiso. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 D.2 Tšehetso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 D.3 Boitsebiso ba khoebo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177

Selelekela
Rea u amohela bukeng ea litaelo bakeng sa roboto e sebetsanang le metsi ea Opentrons Flex. Bukana ena e u tataisa hoo e ka bang ntho e 'ngoe le e' ngoe eo u hlokang ho e tseba ho theha le ho sebelisa Flex, e shebane le lihlooho tse bohlokoa haholo ho basebelisi ba letsatsi le letsatsi ba Flex sebakeng sa laborateng.
Sebopeho sa bukana ena
Opentrons Flex ke sistimi e rarahaneng, kahoo ho na le litsela tse ngata tse fapaneng tsa ho ithuta tsohle tseo e ka li etsang. Ikutloe u lokolohile ho tlolela ka ho toba khaolong e buang ka taba efe kapa efe eo u labalabelang ho e tseba! Bakeng sa mohlalaampLeha ho le joalo, haeba u se u ntse u e-na le Flex e hlophisitsoeng ka laboratoring ea hau, u ka tlōla khaolo ea Ho Kenya le Phalliso.
Haeba u khetha mokhoa oa tataiso, bukana ena e hlophisitsoe hore u tle u e latele ho tloha qalong ho isa qetellong.
Ithute ka Flex. Likarolo tse khethollang tsa Flex li thathamisitsoe Khaolong ea 1: Selelekela. Kenyelletso e boetse e kenyelletsa lintlha tsa bohlokoa tsa polokeho le taolo.
Qala ka Flex. Haeba o hloka ho theha Flex ea hau, latela litaelo tse qaqileng Khaolong ea 2: Ho Kenyeletsoa le Phalliso. Ebe u tloaelana le likarolo tsa Flex Khaolong ea 3: Tlhaloso ea Tsamaiso.
Beha setulo sa hau. Ho hlophisa mokato ho thusa lits'ebetso tse fapaneng tsa mahlale ho Flex. Khaolo ea 4: Li-modules li hlalosa li-peripherals tsa Opentrons tseo u ka li kenyang kapa ka holim'a setulo ho etsa mesebetsi e itseng ea saense. Khaolo ea 5: Labware e hlalosa mokhoa oa ho sebetsa ka lisebelisoa tsa ho ts'oara metsi.
Etsa protocol. Tšebeliso ea mantlha ea Flex e tsamaisa mekhoa e tloaelehileng ea mahlale, e tsejoang ka hore ke liprothokholo. Khaolo ea 6: Nts'etsopele ea Protocol e fana ka mekhoa e mengata ea ho fumana liprothokholo tse lokiselitsoeng kapa ho iketsetsa tsona. Ho tsamaisa protocol ea hau, latela litaelo tse Khaolong ea 7: Software le Ts'ebetso, e nang le litaelo tsa ho etsa mesebetsi e meng le ho etsa litlhophiso tsa roboto ea hau.
Boloka Flex e sebetsa. Latela keletso e Khaolong ea 8: Tlhokomelo le Tšebeletso ho boloka Flex ea hau e hloekile 'me e sebetsa hantle. Kapa ingolise bakeng sa e 'ngoe ea litšebeletso tsa Opentrons tse thathamisitsoeng moo 'me u re lumelle ho u hlokomela ka Flex.
Ithute haholoanyane. U ntse u hloka ntho e 'ngoe hape? Sheba lihlomathiso. Sehlomathiso A: Bukana e hlalosang mantsoe a hlalosang mantsoe a amanang le Flex. Sehlomathiso B: Litokomane tse Eketsehileng li u supisa lisebelisoa tse eketsehileng bakeng sa lihlahisoa tsa Opentrons le khoutu ea ho ngola ho laola Flex.

OPENTRONS FLEX

SEBAKA
Sehlomathiso C: Software ea Open-Source e hlalosa hore na software ea Opentrons e tsamaisoa joang ho GitHub e le sesebelisoa bakeng sa bahlahisi le bao e seng bahlahisi.
Sehlomathiso sa D: Tlhahisoleseding ea Tšehetso le Mehala e thathamisa mokhoa oa ho ikopanya le Opentrons haeba u hloka thuso ho feta seo litokomane tsa rona li fanang ka tsona.
Lintlha le litemoso
Ho pholletsa le bukana ena, u tla fumana lintlha tse hlophisitsoeng ka mokhoa o ikhethileng le li-block tsa temoso. Lintlha li fana ka lintlha tse thusang tse ka 'nang tsa se ke tsa bonahala nakong e tloaelehileng ea ho sebelisa Flex. Ela hloko ka ho khetheha litemoso-li sebelisoa feela maemong ao u ka ipehang kotsing ea ho tsoa kotsi, tšenyo ea lisebelisoa, tahlehelo kapa ho senyeha ha s.amplisebelisoa, tahlehelo ea data, kapa kotsi e 'ngoe. Lintlha le litemoso li shebahala tjena:
Sample Tlhokomeliso: Ena ke ntho eo u lokelang ho e tseba, empa ha e hlahise kotsi leha e le efe.
SampLe Tlhokomeliso: Sena ke ntho eo u lokelang ho e tseba hobane ho na le kotsi e amanang le eona.

OPENTRONS FLEX

KHAOLO EA 1
Selelekela

Khaolo ena e u tsebisa ka Opentrons Flex ecosystem, ho kenyeletsoa moralo oa sistimi ka kakaretso le litlhophiso tse fumanehang tsa sebaka sa mosebetsi. E boetse e kenyelletsa tlhahisoleseding ea bohlokoa ea ho latela melao le polokeho, eo u lokelang ho e tsosolosaview pele o theha roboto ea hau ea Opentrons Flex. Bakeng sa lintlha tse ling mabapi le likarolo tsa Opentrons Flex, bona khaolo ea Tlhaloso ea Sistimi.

1.1 Rea u amohela ho Opentrons Flex
Opentrons Flex ke roboto e sebetsanang le mokelikeli e etselitsoeng tšebetso e phahameng le ho sebetsa ka mokhoa o rarahaneng. Roboto ea Flex ke motheo oa sistimi e modular e kenyelletsang li-pipette, mochini o ts'oarang li-labware, lisebelisoa tsa mekato, li-module tsa on-deck, le labware - tseo kaofela u ka li fetolang. Flex e entsoe ka "touchscreen" hore o tle o sebetse le eona ka kotloloho bencheng ea lab, kapa o ka e laola ho tsoa lab ea hau ka Opentrons App kapa li-API tsa rona tse bulehileng.
Lits'ebetso tsa Flex li tla le lisebelisoa tsohle - roboto, hardware, le labware - tseo u li hlokang ho qala ho iketsetsa mesebetsi e tloaelehileng ea lab. Bakeng sa lits'ebetso tse ling, Opentrons Flex e sebetsa ho software le firmware e bulehileng ka botlalo, 'me ke reagent- le labware-agnostic, e u fang taolo ea hore na u rala le ho tsamaisa liprothocol tsa hau joang.

Ke eng e ncha ho Flex
Opentrons Flex ke karolo ea letoto la liroboto tsa Opentrons tse sebetsanang le metsi. Basebelisi ba Opentrons Flex e kanna ea ba ba tloaelane le Opentrons OT-2, roboto ea rona ea lipeipi. Flex e fetela ka nģ'ane ho bokhoni ba OT-2 libakeng tse 'maloa tsa bohlokoa, e fana ka nako e phahameng ea ho feta le nako ea ho tsamaea.

Feature Pipette throughput
Bokhoni ba Pipette le malebela

Tlhaloso
Li-pipette tsa Flex li na le likanale tse 1, 8, kapa tse 96. Pipette ea 96-channel e sebetsa ka makhetlo a 12 liliba tse ngata ka nako e le 'ngoe ho feta pipette e kholo ka ho fetisisa ea OT-2.
Li-pipette tsa Flex li na le mefuta e meholo ea molumo (1 µL, 50 µL) 'me kaofela li ka sebetsa ka bolumo efe kapa efe ea malebela a Opentrons Flex. Ena ke ntlafatso ho feta liphaephe tsa OT-5, tse nang le mekhahlelo e menyenyane 'me li tlameha ho sebelisa malebela a nang le mefuta e lumellanang ea molumo.

OPENTRONS FLEX

KHAOLO EA 1: SELELEKELA

Gripper Automated calibration Touchscreen Module caddies Deck slot e hokahanya matlakala a tsamaisoang Boholo le boima ba 'mele

Opentrons Flex Gripper e nka le ho tsamaisa labware ho potoloha mokato ka bo eona, ntle le ts'ebetso ea mosebelisi. The gripper e nolofalletsa mosebetsi o rarahaneng haholoanyane ka har'a protocol e le 'ngoe.
Tekanyo ea boemo ba lipeipi tsa Flex le gripper e iketselitse ka botlalo. Tobetsa konopo e le 'ngoe, 'me sesebelisoa se tla ea libakeng tse entsoeng ka mokhoa o nepahetseng mokatong oa sekepe ho fumana hore na e hokae, ho boloka data eo hore e sebelisoe liprothokholeng tsa hau.
Flex e na le sebopeho sa eona sa "touchscreen" se u lumellang hore u se laole ka kotloloho, ntle le ho sebelisa Opentrons App. Sebelisa "touchscreen" ho qala ts'ebetso ea protocol, hlahloba boemo ba mosebetsi, 'me u fetole litlhophiso hantle robotong.
Li-module tsa Flex li kenella ka har'a li-caddies tse lulang sebakeng se ka tlase ho mokato. Li-Caddies li beha labware ea hau haufi le bokaholimo ba mokato 'me u lumelle ho tsamaisa lithapo tse ka tlase ho mokato. Li-Caddies li thusa le ho feta tlhophiso ea module le labware setulong.
Li-slots tsa Deck ho Flex li baloa ka sistimi e hokahanyang (A1D4) e ts'oanang le tsela eo liliba li baloang ka eona ho labware.
Moqomo oa lithōle o ka ea libakeng tse ngata tsa mokatong oa Flex. Sebaka sa kamehla (slot A3) ke sebaka se khothalelitsoeng. U ka sebelisa gripper ho lahla lithōle ka har'a chute ea lithōle eo u ka e khethang.
Flex e batla e le kholoanyane ebile e boima haholo ho feta OT-2. Mesebetsi ea ho kenya Flex e hloka thuso ea molekane oa laboratori.

Papiso e qaqileng ea lintlha tsa tekheniki ea liroboto e fumaneha ho Opentrons websebaka.
Ka bobeli liroboto tsa Flex le OT-2 li sebelisa software ea rona e bulehileng, 'me Opentrons App e ka laola mefuta ka bobeli ea liroboto hang. Le hoja li-protocol tsa OT-2 li ke ke tsa tsamaisoa ka ho toba ho Flex, ho bonolo ho li fetola (sheba karolo ea OT-2 Protocols ea khaolo ea Protocol Development bakeng sa lintlha tse qaqileng).

Lisebelisoa tsa Flex
Lits'ebetso tsa Opentrons Flex li kenyelletsa roboto ea Flex, lisebelisoa, li-pipette le gripper, li-module tsa on-deck, le li-labware tse hlokahalang ho iketsetsa ts'ebeliso e itseng. Likarolo tsohle tsa mochini oa mosebetsi li modular. Haeba o hloka ho fetola lits'ebetso, o ka eketsa kapa oa fapanyetsana ka lisebelisoa tse ling tsa Flex le lisebelisoa tse tsamaellanang.

OPENTRONS FLEX

KHAOLO EA 1: SELELEKELA
NGS MOSEBETSI
Opentrons Flex NGS Workstation e iketsetsa prep ea laebrari ea NGS. E ka iketsetsa phallo ea mosebetsi pele ho tatellano ka ho sebelisa sistimi efe kapa efe e etellang pele ea reagent, ho kenyeletsoa karohano- le tagtlhophiso ea laebrari e thehiloeng ho mantsoe.
Ntle le roboto ea Flex, NGS Workstation e kenyelletsa:
Khetho ea Gripper ea tlhophiso ea pipette
Lipipette tse peli tsa 8-Channel (1 µL le 50 µL) 5-Channel Pipette (1000 µL) Waste Chute Magnetic Block Temperature Module Thermocycler Module Labware kit e nang le malebela a sefe, li-tubes tsa microcentrifuge, li-reservoirs, le lipoleiti tsa PCR.
PCR MOSEBETSI
Opentrons Flex PCR Workstation e iketsetsa PCR setup le thermocycling workflows ho fihlela ho 96 s.amples. E ka aliquot chilled reagents le sampe kenella ka har'a poleiti ea PCR ea seliba tse 96. Ka kenyelletso ea Thermocycler Module e ikemetseng, sebelisa gripper ho kenya poleiti ka har'a Thermocycler, ebe u tsamaisa lenaneo la hau la PCR leo u le khethileng.
Ntle le roboto ea Flex, PCR Workstation e kenyelletsa:
Khetho ea Gripper ea tlhophiso ea pipette
1-Channel Pipette (1 µL) le 50-Channel Pipette (8 µL) 1-Channel Pipette (50 µL) Waste Chute Temperature Module Labware kit e nang le malebela a sefe, li-tubes tsa microcentrifuge, matamo le lipoleiti tsa PCR.

OPENTRONS FLEX

KHAOLO EA 1: SELELEKELA
MOSEBETSI OA NAKO EA ACID
Opentrons Flex Nucleic Acid Extraction Workstation e iketsetsa DNA/RNA ho itšehla thajana le tlhoekiso. E sebelisa Magnetic Block bakeng sa karohano ea lifaha tsa makenete, le Heater-Shaker bakeng sa s.ample lysis le ho emisoa ha lifaha tsa makenete.
Ntle le roboto ea Flex, Nucleic Acid Extraction Workstation e kenyelletsa:
Khetho ea Gripper ea tlhophiso ea pipette
1-Channel Pipette (5 µL) le 1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL) Waste Chute Magnetic Block Heater-Shaker Module Labware kit e nang le malebela a sefe, matamo, lipoleiti tsa seliba tse tebileng tsa PCR
MOSEBETSI OA MAGNETIC BEAD PHETHATSO EA PROTEIN
Setsi sa Opentrons Flex Magnetic Bead Protein Purification Workstation se iketsetsa hloekiso ea liprotheine tse nyane le li-proteomics.ample lokisetsa ho fihlela ho 96 samples. E tsamaisana le li-reagents tse ngata tse tsebahalang tse thehiloeng ho lifaha.
Ntle le roboto ea Flex, Protein Purification Workstation e kenyelletsa:
Khetho ea Gripper ea tlhophiso ea pipette
1-Channel Pipette (5 µL) le 1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL) Waste Chute Magnetic Block Heater-Shaker Module Labware kit e nang le malebela a sefe, matamo, lipoleiti tsa seliba tse tebileng tsa PCR
FLEX PREP MOSEBETSI
Opentrons Flex Prep Workstation e iketsetsa mokhoa o bonolo oa ho tsamaisa lipeipi. Lokisa sebaka sa mosebetsi ka 1-Channel le 8-Channel Pipettes ho etsa mesebetsi e kang s.ample transfer, sample phetiso, le

OPENTRONS FLEX

KHAOLO EA 1: SELELEKELA
reagent aliquoting. Lokisa sebaka sa mosebetsi ka 96-Channel Pipette ho etsa aliquoting ea boemo bo holimo le plate st.ampng.
Ntle le roboto ea Flex, Flex Prep Workstation e kenyelletsa:
Khetho ea tlhophiso ea pipette: 1-Channel Pipette (5 µL) le 1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL)
Labware Kit e nang le malebela a sefa, li-tubes tsa microcentrifuge, le matamo
PLAMID PREP MOSEBETSI
Opentrons Flex Plasmid Prep Workstation e iketsetsa tlhahiso ea plasmid e thehiloeng ho lifaha tsa makenete le phallo ea mosebetsi oa tlhoekiso. Setsi sena sa mosebetsi se na le liphaephe tsa molumo o phahameng, Heater-Shaker Module, le Magnetic Block ho amohela lik'hemik'hale tse ngata tse thehiloeng ho lifaha.
Ntle le roboto ea Flex, Plasmid Prep Workstation e kenyelletsa:
Gripper 1-Channel Pipette (5 µL) le 1000-Channel Pipette (8 µL) Waste Chute Magnetic Block Heater-Shaker Module Labware kit e nang le malebela a sefe, li-tubes tsa microcentrifuge, matamo, lipoleiti tsa PCR, le lipoleiti tse tebileng tsa seliba.
SYNBIO MOSEBETSI
Opentrons Flex SynBio Workstation e iketsetsa mefuta e fapaneng ea liphatlalatso tsa tlhaho tsa tlhaho tse kang DNA synthesis le cloning. E sebelisa Magnetic Block le Temperature Module ho tšehetsa lik'hemik'hale tse ngata tse thehiloeng ho lifaha. Kenya Thermocycler Module ho etsa li-incubations tsa sekoahelo se futhumetseng le amplihlahisoa.
Ntle le roboto ea Flex, SynBio Workstation e kenyelletsa:
Gripper 1-Channel Pipette (5 µL) le 1000-Channel Pipette (8 µL) Magnetic Block Temperature Module

OPENTRONS FLEX

KHAOLO EA 1: SELELEKELA

Labware kit e nang le malebela a kamehla le a sefa, li-tubes tsa microcentrifuge, matamo, lipoleiti tsa PCR, le lipoleiti tse tebileng tsa seliba.

1.2 Litaba tsa polokeho
Roboto ea Opentrons Flex e sebetsanang le metsi e etselitsoe ts'ebetso e sireletsehileng. Sheba lintlha le litataiso tsa ho latela melao karolong ena ho netefatsa ts'ebeliso e bolokehileng ea Flex ea hau. Litaelo tsena li akaretsa ts'ebeliso e bolokehileng ea likhokahano tse kenang le tse hlahisoang bakeng sa sehlahisoa, ho kenyeletsoa likhokahano tsa matla le data, hammoho le lileibole tsa temoso tse fumanoang robotong ea Flex le hardware e amanang le eona. Ho sebelisa sesebelisoa ka tsela e fapaneng le e boletsoeng bukeng ena ho ka beha mosebelisi le thepa kotsing.

Matšoao a tšireletso
Lileibole tse fapaneng ho Flex le bukaneng ena li u lemosa ka mehloli ea kotsi kapa kotsi e ka bang teng.

Letšoao

Tlhaloso
Tlhokomeliso: E lemosa basebelisi maemo a ka bang kotsi. Liketso tse ka bakang kotsi kapa lefu.
Tlhokomeliso: E lemosa basebelisi khahlanong le Tšenyo ea lisebelisoa. Lintlha tse lahlehileng kapa tse senyehileng. Khatiso e ke keng ea hlakoloa ea tšebetso e etsoang.
Motlakase: E hlwaya likarolo tsa lisebelisoa tse ka bakang kotsi ea ho tsosoa ke motlakase haeba sesebelisoa se sa sebetsoe hantle.

Hot surface: E hlwaya likarolo tsa lisebelisoa tse bakang kotsi ea kotsi ea motho ka lebaka la mocheso o phahameng / mocheso haeba sesebelisoa se sa sebetse hantle.

Pinch point: E hlwaya lisebelisoa tse ka bakang kotsi ea ho lemala ha motho a tsamaea.

OPENTRONS FLEX

KHAOLO EA 1: SELELEKELA

U tla fumana mangolo a latelang ho Flex:
Lileibole tsa thepa ea bohlale Lileibole tsa melao ea tsamaiso (mohlala, ETL) Lileibole tsa kotsi ea motlakase Lileibole tsa tlhokomeliso ka kakaretso Lileibole tsa sehlahisoa Pinch point labels High voltage labels Power rating labels

Litemoso tsa polokeho ea motlakase
Kamehla ela hloko litemoso tse latelang tsa polokeho ea motlakase:

Letšoao

Tlhaloso
Hokela roboto potolohong e thehiloeng fatše, ea Sehlopha sa 1. Sheba karolo ea Khokahano ea Matla khaolong ea Tlhaloso ea Tsamaiso.

Se ke oa hokela (plug in), hakolla (qhalla), kapa sebelisa lithapo tsa motlakase tsa AC haeba: Thapo e robehile kapa e senyehile. Lithapo tse ling tse khomaretsoeng, lithapo, kapa lijana tsa ho tšela li ea robeha kapa lia senyeha.
Ho sebelisa lithapo tsa motlakase tse senyehileng ho ka baka kotsi ea ts'oaetso ea motlakase e bakang kotsi e mpe kapa tšenyo ea roboto.
Se ke oa khutlisa thapo ea motlakase ea AC ntle le haeba e le ka tataiso ea Opentrons Support.

Bakeng sa lintlha tse ling mabapi le litlhoko tsa motlakase, bona karolo ea Tšebeliso ea Motlakase khaolong ea ho Kenya le ho Falla.

OPENTRONS FLEX

KHAOLO EA 1: SELELEKELA

Litemoso tse ling tsa polokeho
Kamehla ela hloko litemoso tse latelang tsa polokeho:

Letšoao

Tlhaloso
Opentrons Flex ha e so netefatsoe hore e ka sebelisoa le lino tse ka qhomang kapa tse tukang. Se ke oa kenya lipoleiti, litšupu, kapa linkho tse nang le maro a ka phatlohang kapa a ka tukang ka har'a roboto kapa ka tsela e 'ngoe sebelisa sesebelisoa ka maro a ka phatlohang kapa a ka tukang sebakeng se kampetsoeng.
Sebelisa mekhoa e metle ea laboratori 'me u latele litemoso tsa moetsi ha u sebetsa ka lik'hemik'hale. Opentrons ha e ikarabelle kapa e ikarabella bakeng sa tšenyo leha e le efe ka lebaka la, kapa ka lebaka la tšebeliso ea lik'hemik'hale tse kotsi.

The Flex e boima ba lik'hilograma tse 88.5 (195 lbs). Ka lebaka leo, ho hloka batho ba babeli ho e phahamisa le ho e tsamaisa ka mokhoa o sireletsehileng. Sheba karolo ea Phalliso khaolong ea Tlhophiso le Phalliso.

The Flex e lokela ho behoa holim'a sebaka se khonang ho tšehetsa boima ba eona ba 88.5 kg (195 lbs) e nang le sebaka se lekaneng sa ho amohela roboto hammoho le sebaka sa eona se fokolang sa sebaka (20 cm / 8 in). Sheba karolo ea Litlhoko tsa Tšireletseho le Ts'ebetso khaolong ea Tlhophiso le Phalliso.
Flex e ka ntša ho thothomela ha e ntse e sebetsa. Beha roboto sebakeng se tiileng, se leka-lekaneng, 'me se sa keneleng metsi se nang le li-cross-bracing kapa manonyeletso a cheselitsoeng. Sheba karolo ea Litlhoko tsa Tšireletseho le Ts'ebetso khaolong ea Tlhophiso le Phalliso.

Litlhokomeliso tsa polokeho
Ho thusa ho sireletsa Flex ts'enyong, latela litemoso tsena:

OPENTRONS FLEX

KHAOLO EA 1: SELELEKELA

Letšoao

Tlhaloso
Sebelisa labware e lumellanang le ANSI/SLAS kapa e amohetsoeng ke Opentrons. Sheba khaolo ea Labware.
Boloka lintho tse senyang, liakhente, kapa lintho tse ling tse senyang hole le roboto.

Tšireletseho ea likokoana-hloko
Tšoara mehlala le li-reagents tse nang le lisebelisoa tse nkiloeng ho batho e le lintho tse ka tšoaetsanoang. Opentrons e khothalletsa ho sebelisa mekhoa e sireletsehileng ea laboratori joalokaha e hlalositsoe ho Biosafety in Microbiological and Biomedical Laboratories (BMBL) 6th Edition.
Tlas'a maemo a tloaelehileng, Flex ha e thehe li-aerosol tse bonoang ho tsoa ho metsi a mohloli. Leha ho le joalo, tlas'a maemo a itseng, hoa khoneha ho hlahisa li-aerosols ho tsoa ho metsi a mohloli. Ha o sebetsa ka biosafety level 2 kapa metsi a tsoang mohloling o moholoanyane, nahana ka ho nka mehato ea ho itšireletsa khahlanong le ho pepesehela aerosol, ho latela mekhatlo ea taolo ea lehae ea hau. Ho fokotsa kotsi e ka bang teng ea ho pepesehela aerosol ho tsoa ho roboto, etsa bonnete ba hore u:
Etsa tlhokomelo joalokaha ho hlalositsoe khaolong ea Tlhokomelo le Tšebeletso. Kenya hantle 'me u sireletse likoahelo tsohle tsa lisebelisoa, li-pipette, li-module le labware. Sebelisa mokhoa o nepahetseng oa liphaephe ho thusa ho fokotsa li-aerosols.
Mosi o chefo
Haeba u sebetsa ka lihlapolli tse sa fetoheng kapa lintho tse chefo, sebelisa mokhoa o sebetsang oa laboratori oa ho kenya moea ho tlosa mouoane leha e le ofe o ka hlahisoang.
Mekelikeli a tukang
Flex ha e so hlahlojoe hore e sebelisoe ka lino tse tukang 'me ha ea lokela ho sebelisoa ka lino tse tukang.

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KHAOLO EA 1: SELELEKELA

1.3 Ho latela melao
Opentrons Flex e lumellana le litlhoko tsohle tse sebetsang tsa litekanyetso tse latelang tsa polokeho le tsa motlakase.

Polokeho
Molao ID IEC/UL/CSA 61010-1 IEC/UL/CSA 61010-2-051

Sehlooho
Litlhoko tsa Polokeho Bakeng sa Thepa ea Motlakase bakeng sa Tekanyo, Taolo, le Tšebeliso ea Laboratori Karolo ea 1: Litlhoko tse akaretsang.
Litlhoko tse khethehileng tsa lisebelisoa tsa laboratori bakeng sa ho kopanya le ho hlohlelletsa

Tšebelisano ea motlakase

ID ea molao EN/BSI 61326-1
FCC 47 CFR Karolo ea 15 Karolo e Nyenyane ea B Sehlopha sa A IC ICES-003

Sehlooho
Thepa ea Motlakase bakeng sa Tekanyo, Taolo, le Litlhoko tsa Tšebeliso ea Lab ea EMC Karolo ea 1: Litlhokahalo tse Akaretsang
Tšitiso ea Tsamaiso ea Mahlale le Mehala e Bakang Thepa e Tloaelehileng ea Theknoloji ea Boitsebiso (Ho kenyeletsoa Digital Apparatus)

Litemoso le lintlha tsa FCC
Tlhokomeliso: Liphetoho kapa liphetoho yuniti ena e sa amoheloang ka ho hlaka ke Opentrons li ka hlakola matla a mosebelisi a ho sebelisa sesebelisoa.
Sesebelisoa sena se lumellana le Karolo ea 15 ea melao ea FCC. Ts'ebetso e ipapisitse le tse latelang:

OPENTRONS FLEX

KHAOLO EA 1: SELELEKELA
Sesebelisoa sena se ka 'na sa se ke sa baka tšitiso e kotsi. Sesebelisoa sena se tlameha ho amohela tšitiso efe kapa efe e amohetsoeng, ho kenyelletsa le tšitiso e ka bakang ho sa lakatseheng
ts'ebetso.
Ela hloko: Thepa ena e lekotswe mme e fumanwe e dumellana le meedi ya sesebediswa sa dijithale sa Sehlopha sa A, ho latela Karolo ya 15 ya melao ya FCC. Meeli ena e etselitsoe ho fana ka tšireletso e utloahalang khahlanong le tšitiso e kotsi ha thepa e sebelisoa sebakeng sa khoebo. Thepa ena e hlahisa, e sebelisa, 'me e ka khantša matla a maqhubu a seea-le-moea, 'me haeba e sa kengoa le ho sebelisoa ho latela bukana ea litaelo, e ka baka tšitiso e kotsi likhokahanong tsa radio. Ts'ebetso ea lisebelisoa tsena sebakeng sa bolulo e kanna ea baka tšitiso e kotsi moo mosebelisi a tla tlameha ho lokisa tšitiso ka litšenyehelo tsa bona.
Canada ISED compliance
Canada ICES-003(A) / NMB-003(A)
Sehlahisoa sena se kopana le lintlha tse sebetsang tsa Boqapi, Saense le Ntlafatso ea Moruo Canada.
Le présent produit est conforme aux specifications techniques applys d'Innovation, Sciences et Developpement économique Canada.
Temoso ea tikoloho
Tlhokomeliso: Mofets'e le Kotsi ea Pelehi www.P65Warnings.ca.gov
Setifikeiti sa Wi-Fi
Mojule oa Wi-Fi o netefalitsoe esale pele hore o sebelisoe libakeng tse ngata:
United States (FCC): FCC Identifier UAY-W8997-M1216 European Economic Area (CE): Ha ho sesupo sa sechaba (boiphatlalatso) Canada (IC): Hardware Version Identification Number W8997-M1216 Japan (TELEC): Nomoro e netefalitsoeng 020-170034 India (WPC): Nomoro ea ngoliso ETA-SD-20191005525 (boiphatlalatso)

OPENTRONS FLEX

KHAOLO EA 2
Kemiso le Phalliso
Khaolo ena e hlalosa mokhoa oa ho lokisa lab ea hau bakeng sa Opentrons Flex, mokhoa oa ho theha roboto, le mokhoa oa ho e tsamaisa ha ho hlokahala. Pele o tlisa Flex ea hau, etsa bonnete ba hore lab kapa setsi sa hau se fihlela litekanyetso tsohle tse karolong ea Safety and Operating Requirements. Ha e le nako ea ho lokisa Flex ea hau, latela litaelo tse qaqileng tse karolong ea Unboxing, First Run, le Instrument Installation le Calibration, kapa sebelisa tšebeletso ea Opentrons Onsite Support Set Up. 'Me haeba u hloka ho fallisetsa Flex sebakeng se secha, haufi kapa hole, latela mehato e karolong ea Phalliso.
2.1 Litlhoko tsa polokeho le ts'ebetso
Moo u ka behang Opentrons Flex
Sebaka ke thepa ea bohlokoa hoo e ka bang lab e 'ngoe le e 'ngoe. Flex ea hau e tla hloka tse ling - empa eseng tse ngata haholo, kaha e etselitsoe ho lekana halofo ea benche e tloaelehileng ea lab. Etsa bonnete ba hore u na le sebaka se lumellanang le lintlha tse latelang.
Sebaka sa benche: Sebaka se emeng, se tiileng, se leka-lekaneng, se sa keneleng metsi. Litafole kapa libenche tse nang le mabili (esita le mabili a notlellang) ha li khothalletsoe. Flex e tsamaea ka potlako 'me e na le boima bo bongata, bo ka sisinyehang kapa bo sa leka-lekaneng litafole tse bobebe kapa tse tsamaeang.
Boima ba 'mele: Roboto feela e boima ba 88.5 kg (195 lb) 'me e lokela ho phahamisoa feela ke batho ba babeli ba sebetsang hammoho. Beha roboto sebakeng se ka khonang ho tšehetsa boima ba eona le boima ba limojule life kapa life, labware, lino, kapa lisebelisoa tse ling tsa laboratori tse tla sebelisoa lits'ebetsong tsa hau.
Sebaka sa tšebetso: Litekanyo tsa motheo tsa roboto ke 87 cm W x 69 cm D x 84 cm H (hoo e ka bang 34 ″ x 27″ x 33 ″). Flex e hloka 20 cm (8 ″) ea tumello ea lehlakore le ka morao bakeng sa likhoele, likhokahano tsa USB, le ho ntša mosi o tsoang ho li-module tse futhumatsang le tse pholileng.
Tlhokomeliso: U se ke ua beha mahlakore kapa ka morao ho Flex flush khahlanong le lebota.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA

84cm 33 ″

87cm 34 ″
Opentrons Flex litekanyo tsa motheo.

69cm 27 ″

20cm 8 ″

Holimo view ea Opentrons Flex, e bonts'ang sebaka se tlase le se ka morao.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA

Tšebeliso ea matla
Opentrons Flex e lokela ho hokeloa leboteng sebakeng sa benche kapa haufi le moo o e kenyang teng. Hokela Flex feela ho lipotoloho tse ka amohelang matla a eona a phahameng ka ho fetesisa:
Matla a kenyang: 36 VDC, 6.1 A Tšebeliso e sa sebetseng: 30 W Tšebeliso e tloaelehileng: 40 W (nakong ea tsamaiso ea protocol) Tšebeliso e phahameng: Hoo e ka bang 50 W
Tšebeliso e nepahetseng ea matla e itšetlehile ka:
Chelete le mofuta oa motsamao o etsoang nakong ea protocol. Nako eo roboto e e qetang e sa sebetse. Boemo ba mabone robotong. Ke liletsa tse kae tse hokeletsoeng.
Hopola ho ikarabella bakeng sa lisebelisoa tse ling tsa elektroniki tse sebelisang matla a potoloho e le 'ngoe, ho kenyelletsa le li-module tsa Flex tse nang le lisebelisoa tsa tsona tsa motlakase. Bakeng sa mohlalaample, Thermocycler Module e na le matla a mangata a sebelisoang (630 W) a maholo ho feta roboto ea Flex ka boeona. Haeba ho hlokahala, buisana le molaoli oa setsi sa hau ho etsa bonnete ba hore se kopana le litlhoko tsa motlakase tsa sesebelisoa sa hau.

Maemo a tikoloho
Maemo a tikoloho bakeng sa tšebeliso e khothaletsoang, tšebeliso e amohelehang, le polokelo ea fapana:

E khothalelitsoe ts'ebetsong ea sistimi

E amohelehang bakeng sa ts'ebetso ea tsamaiso

Mocheso oa tikoloho +20 ho +25 °C

+2 ho ea ho +40 °C

Mongobo o amanang Bophahamo

40%, e sa fokotseng
Hoo e ka bang 500 m ka holim'a bophahamo ba leoatle

30%, e sa fokotseng (ka tlase ho 80 °C)
Ho fihlela ho 2000 m ka holim'a bophahamo ba leoatle

Polokelo le lipalangoang
-10 ho ea ho +60 °C
10%, e sa fokotseng (ka tlase ho 85 °C)
Ho fihlela ho 2000 m ka holim'a bophahamo ba leoatle

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
Opentrons e netefalitse ts'ebetso ea Opentrons Flex maemong a khothaletsoang bakeng sa ts'ebetso ea sistimi, mme ts'ebetso maemong ao e lokela ho fana ka liphetho tse nepahetseng. Flex e bolokehile hore e ka sebelisoa maemong a amohelehang bakeng sa ts'ebetso ea sistimi, empa liphetho li ka fapana. Se ke oa kenya matla kapa oa sebelisa Flex maemong a kantle ho meeli eo. Maemo a polokelo le lipalangoang a sebetsa feela ha roboto e khaotsoe ka ho feletseng ho tloha motlakase le lisebelisoa tse ling.
2.2 Unboxing
Kea u babatsa! Opentrons Flex ea hau e fihlile 'me u e lokiselitse sebaka ka laboratoring ea hau. Ha re bule kereiti eo ea monster, re tlose roboto, 'me re e lokisetse ho sebetsa. Lintlha tse karolong ena li fana ka lethathamo la likarolo le litaelo tse u tsamaisang mehatong e hlokahalang ho etsa hore Flex e senngoe ka lebokoseng, e hlophisoe, 'me e loketse ho sebelisoa. Re arotse mokhoa oa ho seta likarolo tse tharo:
Karolo ea 1 e akaretsa ho qhaqholla kreiti. Karolo ea 2 e akaretsa ho tlosa Flex ho tloha ka kreiting le ho e isa sebakeng sa ho qetela sa kopano. Karolo ea 3 e akaretsa kopano ea ho qetela le ho kenya matla ho roboto ka lekhetlo la pele.
Boiteko le nako e hlokahalang
U tla batla ho kopa molekane oa lab ho thusa ka ts'ebetso ea unboxing, ho phahamisa, ho falla le ho kopanya. U tla hloka ho etsa tekanyetso ea metsotso e ka bang 30 ho isa ho hora bakeng sa boiteko bona.
Tlhokomeliso: Flex e hloka batho ba babeli ho e phahamisa hantle. Hape, ho phahamisa le ho jara Flex ka matsoho a eona ke tsela e molemohali ea ho tsamaisa roboto.
Kereiti le thepa ea ho paka
Ho notlolla Flex ho u fa roboto e ntle haholo, empa hape u siiloe ka liphanele tse kholo tsa crate hammoho le likarolo tse fapaneng tsa thomello le padding. Le hoja u ka lahla thepa ena, re u khothalletsa hore u boloke lintho tsena haeba sebaka sa polokelo se le teng. Sephutheloana se ka sebelisoa hape, se thusang ho lokisa Flex ea hau bakeng sa ho romelloa haeba u hloka ho e romela sebakeng se seng (mohlala, kopanong kapa setsing se secha) nakong e tlang.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
Lintlha tsa sehlahisoa
The Flex likepe tse nang le likarolo tse thathamisitsoeng ka tlase. Li-pipettes, gripper, le modules li tla ka liphutheloana tse arohaneng ho tloha ka har'a crate e kholo ea Flex, leha u li rekile hammoho e le setsi sa mosebetsi.

(1) Roboto ea Opentrons Flex

(1) Thapo ea USB

(1) Thapo ea motlakase

(1) Thapo ea Ethernet

(5) Linotlolo tsa L (12 mm hex, 1.5 mm hex, 2.5 mm hex, 3 mm hex,
T10 Torx)

(1) Emergency Stop Pendant

(1) Deck slot e nang le likarolo tsa labware

(4) Likotwana tsa li-labware tse bolokiloeng

(1) Pipette calibration probe

(4) Ho nka lihape le likatiba

(1) Phanele ea fensetere e kaholimo

(4) Liphanele tsa lifensetere tse ka mahlakoreng

(1) 2.5 mm hex screwdriver

(1) 19 mm wrench

(16 + li-spea) Li-screws tsa lifensetere (hlooho e bataletseng ea M4x8 mm)
26

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
Karolo ea 1: Tlosa kreiti
Opentrons e romella Flex ea hau ka kreiti e tiileng ea plywood. Kreiti ea likepe e sebelisa hook le latch clamps ho tiisa liphanele tse ka holimo, tse ka holimo le tse ka tlase hammoho. Ho sebelisa li-latches, sebakeng sa lipekere kapa lisekere, ho bolela hore ha u na ho hloka crowbar (kapa matla a mangata) ho qhaqha kreiti, 'me u ka e kopanya hape hamorao, ha ho hlokahala.
Tlhokomeliso: Likarolo tsa crate li ka senyeha nakong ea thomello. U ka 'na ua batla ho sebelisa li-gloves tsa mosebetsi ho sireletsa matsoho a hau ho tloha ho li-splinters tsa lehong.
Ho lokolla li-latches, flip the latch holimo 'me u e fetole ka ho le letšehali (counterclockwise). Ketso ena e tsamaisa clamp letsoho ho tsoa ka har'a borakete e tsamaisanang le eona. Joale o ka suthisetsa letsoho la latch hole le kreiti.
1 Notlolla lilache tse robeli tse tšoereng bokaholimo ka mahlakoreng.

2 Ntsha phanele e ka hodimo ka mora ho lokolla diletjhe.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FIHLELA 3 Seha mokotla o moputsoa oa ho tsamaisa thepa, tlosa lintho tsena ho padding, 'me u li behe ka thōko:
User Kit Power, Ethernet, le lithapo tsa USB Emergency Stop Pendant
4 Tlosa karolo e ka holimo ea foam padding ho pepesa liphanele tsa lifensetere. Padding e sireletsa mahlakoreng le mahlakoreng a holimo.
5 Ntša liphanele tsa lifensetere ’me u li behelle ka thōko. U tla li hokela hamorao.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
6 Notlolla lilache tse setseng tse 16 tse tšoereng liphanele tse ka mahlakoreng le botlaaseng ba kreiti. 7 Tlosa diphanele tse mahlakoreng mme o di behelle ka thoko.
Karolo ea 2: Lokolla Flex
Ka mor'a ho qeta mehato e Karolong ea 1, joale u lokela ho bona roboto e ka mokotleng o sireletsang 'me e khomaretsoe likarolong tsa tšepe ea lamunu. Mokotla o kenya roboto ebe o e sireletsa tikolohong e kantle. Li-brackets tsa tšepe li sireletsa roboto ho ea tlase ho crate. Liforeimi tse peli tse tsamaisang thepa li tšehetsa roboto, li arola boima ba eona ka ho lekana, 'me li e boloka e tiile hore e se ke ea thekesela nakong ea sekepe. Tsoela pele ho notlolla Flex ebe o e tlosa setsing sa crate.
8 U sebelisa sekoahelo sa 19 mm ho tswa ho User Kit, notlolla masakaneng ho tloha tlase kreiteng. U ka lahla masakana, kapa ua li boloka bakeng sa tšebeliso ea nako e tlang.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FIHLELA 9 Hula kapa u theole mokotla o tsamaisang thepa ho theosa ho ea tlase ho pepesa roboto eohle.
10 Ka thuso ho tsoa ho molekane oa hau oa laborateng, tšoara lintho tse tšoarang matsoho liforeiming tse lamunu tsa thomello ka mahlakoreng a mabeli a motheo oa roboto, phahamisa Flex ho tloha botlaaseng ba crate, 'me u e behe fatše. Boloka kapa u lahle motheo oa krete le foreimi ea thomello.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FIHLELA 11 U sebelisa senotlolo sa L-12 mm hex ho tswa ho User Kit, tlosa liboutu tse 'nè tse tšoereng liforeimi tsa thepa ho.
ea Flex. Boloka kapa u lahle liforeimi le liboutu.
12 Tlosa lihapi tse 'nè tsa aluminium ho tswa ho User Kit. Sekelletsa lihape libakeng tse tšoanang tse neng li tšoere liboutu tsa foreimi ea thomello ea 12 mm.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
13 Ka thuso ea molekane oa hau oa laborateng, phahamisa Flex ka meharo ea eona 'me u e ise setulong sa mosebetsi bakeng sa kopano ea ho qetela.

Karolo ea 3: Kopano ea ho qetela le matla a ho sebetsa
Ka mor'a hore u fallise Flex sebakeng sa mosebetsi oa nakoana, kapa lehae la eona la ka ho sa feleng, ke nako ea ho beha lintlha tsa ho qetela ho roboto ea hau e ncha.
14 Haeba u tsamaisitse roboto sebakeng sa eona sa ho qetela, sa ho sebetsa, tlosa lithupa tsa ho jara 'me u li nkele sebaka ka li-caps tsa ho qetela. Likatiba li koala masoba a mofeng foreiming ebe li fa roboto ponahalo e hloekileng. Khutlisetsa lihape ho User Kit bakeng sa polokelo.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
15 Khutlisetsa liphanele tse ka holimo le tse ka mahlakoreng ho tloha foam ea ho paka eo u e beheletseng ka thōko ka mor'a ho tlosa bokaholimo ba krete.
16 Lokisa liphanele tsa lifensetere ho Flex ka ho latela lintlha tse ngotsweng filiming e ka pele e sireletsang. Ebe u tlosa filimi e sireletsang.
17 U sebelisa li-screw tsa lifensetere tse betliloeng le screwdriver ea 2.5 mm ho tloha ho User Kit, hokela liphanele tsa lifensetere ho Flex. Etsa bonnete ba hore masoba a beveled (e bōpehileng joaloka V) lifensetereng li shebile ka ntle (ho uena). Sena se lumella li-screws hore li lumellane le bokaholimo ba fensetere.

Tlhokomeliso: Ho lebisa liphanele ka tsela e fosahetseng ho ka baka tšenyo. Torque e feteletseng ea screw e ka qhetsola liphanele. Matlafatsa li-screws ho fihlela lifensetere li sireletsehile ka mokhoa o utloahalang. Sena ha se teko ea matla.

OPENTRONS FLEX

KHAOLO 2: HO KENYA LE HO FIHLELA 18 U sebelisa screwdriver ea 2.5 mm ho tswa ho User Kit, tlosa li-screws tsa ho notlela ka har'a gantry. Tsena
li-screw tse thibelang kolotsana ho sisinyeha ha e ntse e tsamaea. Li-screws tsa ho notlela tsa gantry li teng: Ka lehlakoreng le letšehali la terene haufi le ka pele ho roboto. Ka tlas'a letsoho le otlolohileng la gantry. Ka lehlakoreng le letona la terene e haufi le ka pele ho roboto ka har'a bracket ea lamunu. Ho na le li-screw tse peli mona.
Gantry e tsamaea habonolo ka letsoho ka mor'a ho tlosa li-screw tsohle tsa sekepe. 19 Khaola 'me u tlose lihlopha tse peli tsa rabara tse bolokang moqomo oa lithōle sebakeng sa ho tsamaisa thepa.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
20 Hokela thapo ea motlakase ho Flex 'me u e hokele ka har'a lebota. Etsa bonnete ba hore sebaka sa mokato ha se na litšitiso. Fetola sesebelisoa sa motlakase se ka morao ho le letšehali la roboto. Hang ha e buletsoe, gantry e fallela sebakeng sa eona sa lehae 'me skrine ea ho ama e bontša litaelo tse eketsehileng tsa tlhophiso.

Kaha joale Flex ea hau e tsoile ka lebokoseng 'me e se e loketse ho tsamaea, tsoela pele ho ea Karolong ea Pele ea ho Run ka tlase.
2.3 Lebelo la pele
Etsa tlhophiso ea mantlha skrineng sa ho ama pele o hokela lisebelisoa tse ling ho Flex ea hau. Roboto e tla u tataisa ka ho hokela marang-rang a lab, ho ntlafatsa software ea morao-rao, le ho iketsetsa Flex ka ho e fa lebitso.
Matlafatsa
Ha o sebelisa Flex, logo ea Opentrons e tla hlaha skrineng sa ho ama. Kamora metsotsoana e seng mekae, e tla bonts'a skrine ea "Welcome to your Opentrons Flex".
Skrine e amohelehang ea Opentrons Flex. U lokela ho bona skrine sena feela ha u qala Flex ea hau ka lekhetlo la pele.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
Hokela ho netweke kapa komporo
Latela likhothaletso tse skrineng sa ho ama ho hokela roboto ea hau hore e tsebe ho bona lintlafatso tsa software le ho amohela protocol files. Ho na le mekhoa e meraro ea khokahano: Wi-Fi, Ethernet, le USB.

Likhetho tsa khokahano ea marang-rang. U hloka ho ba le khokahano ea inthanete ho theha Flex. Wi-Fi: Sebelisa skrine ea ho ama ho hokela ho marang-rang a Wi-Fi a sirelelitsoeng ka netefatso ea WPA2 Personal (maqhubu a mangata a hlokang password feela ho kena a oela tlas'a sehlopha sena).
Tlhokomeliso: Flex ha e tšehetse li-portal tse hapuoeng (marang-rang a se nang phasewete empa a kenya a webleqepheng la ho netefatsa basebelisi ka mor'a ho hokela).
U ka boela ua hokahanya le marang-rang a bulehileng a Wi-Fi, empa sena ha se khothalletsoe.
Tlhokomeliso: Ho hokela marang-rang a bulehileng a Wi-Fi ho tla lumella mang kapa mang ea sebakeng sa leqhubu la marang-rang ho laola roboto ea hau ea Opentrons Flex ntle le netefatso.

Haeba o hloka ho hokela marang-rang a Wi-Fi a sebelisang netefatso ea khoebo (ho kenyeletsoa "eduroam" le marang-rang a tšoanang a thuto a hlokang lebitso la mosebelisi le password), qala ka ho hokela ho Opentrons App ka Ethernet kapa USB ho phethela ho seta. Ebe u hokela ho netweke ea Wi-Fi ea khoebo ho li-setting tsa marang-rang bakeng sa Flex ea hau. Ho fihlella litlhophiso tsa marang-rang:

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
1. Tobetsa Lisebelisoa ka lehlakoreng le letšehali la Opentrons App. 2. Tobetsa lethathamo la matheba a mararo () bakeng sa Flex ea hau ebe u khetha Litlhophiso tsa Robot. 3. Tobetsa tab ya Networking.
Khetha marang-rang a hau ho tsoa ho menu e theoha kapa u khethe "Kena le marang-rang a mang ..." ebe u kenya SSID ea eona. Khetha mokhoa oa netefatso oa khoebo oo marang-rang a hau a o sebelisang. Mekhoa e sebelisoang ke:
EAP-TTLS e nang le TLS EAP-TTLS e nang le MS-CHAP v2 EAP-TTLS e nang le MD5 EAP-PEAP e nang le MS-CHAP v2 EAP-TLS
E 'ngoe le e 'ngoe ea mekhoa ena e hloka lebitso la mosebelisi le phasewete, 'me ho ipapisitsoe le tlhophiso ea marang-rang e ka hloka setifikeiti files kapa likhetho tse ling. Sheba litokomane tsa IT tsa setsi sa hau kapa ikopanye le mookameli oa hau oa IT bakeng sa lintlha tse mabapi le ho seta marang-rang a hau.
Ethernet: Hokela roboto ea hau ho sesebelisoa sa marang-rang kapa hub ka thapo ea Ethernet. U ka hokela ka kotloloho boema-kepeng ba Ethernet komporong ea hau, ho qala ka mofuta oa 7.1.0 oa roboto.
USB: Hokela thapo e fanoeng ea USB A-to-B boema-kepeng ba roboto ba USB-B le koung e bulehileng komporong ea hau. Sebelisa thapo ea USB B-to-C kapa adaptara ea USB A-to-C haeba komporo ea hau e se na boema-kepe ba USB-A.
Ho tsoela pele ka ho seta, komporo e hoketsoeng e tlameha ho ba le Opentrons App e kentsoeng le ho sebetsa. Bakeng sa lintlha tse mabapi le ho kenya Opentrons App, bona karolo ea App Installation khaolong ea Software le Operation.
Kenya liapdeite tsa software
Kaha joale u se u hokahane le marang-rang kapa k'homphieutha, roboto e ka hlahloba lisebelisoa tsa software le firmware le ho li jarolla ha ho hlokahala. Haeba ho na le ntlafatso, ho ka nka metsotso e seng mekae ho kenya. Hang ha ntlafatso e phethiloe, roboto e tla qala hape.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
Hokela Pendant ea Emergency Stop
Hokela Emergency Stop Pendant (E-stop) e kenyellelitsoeng boema-kepeng ba thusang (AUX-1 kapa AUX-2) ka morao ho roboto.

Pele le ka morao ho hokela Emergency Stop Pendant.
Ho hokela le ho nolofalletsa E-stop hoa tlama bakeng sa ho hokela lisebelisoa le ho tsamaisa liprothokholo ho Flex. Ho fumana lintlha tse ling mabapi le ho sebelisa E-stop nakong ea ts'ebetso ea roboto, bona karolo ea Emergency Stop Pendant ea khaolo ea Tlhaloso ea Tsamaiso.
Fana ka roboto ea hau lebitso
Ho reha lebitso la roboto ea hau ho u thusa ho e tseba habonolo sebakeng sa laboratoring ea hau. Haeba u na le liroboto tse ngata tsa Opentrons marang-rang a hau, etsa bonnete ba hore u li fa mabitso a ikhethang. Hang ha u se u netefalitse lebitso la roboto ea hau, u tla isoa ho Opentrons Flex Dashboard ea hau. Mohlomong mohato o latelang oo u tla batla ho o nka ke ho hokela lisebelisoa, tse tšohliloeng karolong e latelang.
2.4 Ho kenya lisebelisoa le ho lekanya
Kamora ho seta liroboto tsa pele, mohato o latelang ke ho hokela lisebelisoa ho roboto le ho li lekanya.
Ho kenya sesebediswa, tlanya pele ho Instruments skrineng sa ho thetsa kapa u ee karolong ea Pipettes le Modules skrineng sa lintlha tsa sesebelisoa ho Opentrons App. Khetha thaba e se nang letho ebe u khetha Hokela Pipette kapa Hokela Gripper. Haeba thaba eo u batlang ho e sebelisa e se e ntse e tšoaretsoe, u lokela ho tlosa pipette kapa gripper pele.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
Tlhokomeliso: Ts'ebetso ea ho kenya ka kakaretso ea ts'oana ho sa tsotelehe hore na o sebelisa sekirini sa ho ama kapa Opentrons App. Sesebediswa sefe kapa sefe seo o qalang ho sona se tla laola tshebetso ya ho instola ho fihlela o se qetile kapa o se hlakola.
Haeba o qala skrineng sa ho ama, sesebelisoa se tla bonts'a roboto e le "maphathaphathe". Haeba o qala ho sesebelisoa, "touchscreen" e tla bonts'a modal e bonts'ang hore ho kenya sesebelisoa ho ntse ho tsoela pele.
Ts'ebetso e nepahetseng ea ho kenya e fapana ho latela sesebelisoa seo u se hokelang, joalo ka ha ho akarelitsoe likarolong tse ka tlase. Lisebelisoa tsohle li na le mokhoa o ikemetseng oa calibration, oo u lokelang ho o etsa hang ka mor'a ho kenya.
Ho kenya Pipette
Ha o kenya pipette, o tla tataisoa ho latela mehato e latelang skrineng sa ho ama kapa ho Opentrons App.
1. KHETHA PIPETTE TYPE Khetha pakeng tsa 1- kapa 8-Channel Pipette le 96-Channel Pipette. Ho hokela Pipette ea 96-Channel ho hloka mehato e 'maloa e eketsehileng hobane e khomaretse poleiti e khethehileng ea ho kenya e pota-potileng li-pipette tse peli.
2. LOKISETSA HO INSTALLATION Tlosa labware ho tloha mokatong 'me u hloekise sebaka sa ho sebetsa ho etsa hore ho be bonolo ho kopanya le ho lekanya. Hape bokella lisebelisoa tse hlokahalang, joalo ka calibration probe, hex screwdriver, le mounting plate (bakeng sa 96-Channel Pipette).
3. HLOKA LE HO SIRELETSA PIPETTE The gantry e tla fallela ka pele ho roboto e le hore u ka hokela pipette.
Li-pipettes tsa 1- le 8-Channel li hokahana ka kotloloho sebakeng sa pipette. Pipette ea 96-Channel e hloka poleiti e ntseng e hola. Ho hokela mounting plate, o tlameha ho qala ka ho hakolla kariki ea z-axis bakeng sa ho homa pipette e nepahetseng.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
Kopanya pipette sebakeng se khethiloeng sa pipette 'me u sireletse li-screws tsa eona.
4. SEBELISA KHALIBRI E TSAMAISITSENG Ho lekanya pipette, hokela probe ea calibration ho nozzle ea pipette e loketseng. Pipette e tla itsamaela ka bo eona ho ama lintlha tse itseng mokatong le ho boloka litekanyetso tsena tsa ho lekanya bakeng sa tšebeliso ea nako e tlang. Hang ha tlhahlobo e phethiloe 'me u tlositse probe, pipette e tla be e loketse ho sebelisoa ho liprothokholo.
Ho kenya Gripper
Ha o kenya gripper, o tla tataisoa ke mehato e latelang skrineng sa ho ama kapa ho Opentrons App.
1. LOKISETSA HO INSTALLATION Tlosa labware ho tloha mokatong 'me u hloekise sebaka sa ho sebetsa ho etsa hore ho be bonolo ho kopanya le ho lekanya. Hape bokella hex screwdriver e hlokahalang 'me u netefatse hore pin ea calibration e sebakeng sa eona sa polokelo holim'a gripper.
2. HOKAHA 'ME U SIRELETSE GRIPPER Sekoti se tla ea ka pele ho roboto e le hore u khone ho hokela gripper. Hokela gripper ho thaba ea katoloso 'me u sireletse li-screws tsa eona.
3. REN AUTOMATED CALIBRATION Ho lekanya gripper, kenya phini ea calibration mohlahareng o ka pele. gripper e tla itsamaela ka bo eona ho ama lintlha tse itseng mokatong oa sekepe ebe e boloka boleng bona ba ho lekanya hore e tle e sebelisoe nakong e tlang. Ebe u pheta ts'ebetso e ts'oanang ka pini ea calibration ka mohlahare o ka morao. Hang ha tlhahlobo e phethiloe 'me u khutlisitse pinana sebakeng sa eona sa polokelo, gripper e tla be e loketse ho sebelisoa ho liprothokholo.
2.5 Ho falla
Karolo ena e fana ka likeletso le litaelo mabapi le mokhoa oa ho tsamaisa roboto ea hau ea Opentrons Flex maetong a makhutšoane le a malelele.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
Metsamao e mekgutshwane
Ho tsamaea hakhutšoane ho nka sebaka se fapaneng ho tloha “ha re e tsamaiseng hanyane” ho ea ho lab, ho theosa le holo, kapa mokatong o mong mohahong oa hau. Maemong ana, o ka tsamaisa Flex ea hau ka letsoho. Ho e tsamaisa ka kariki ea letsoho le eona ke khetho e ntle.
Tlhokomeliso: The Flex e boima ba lik'hilograma tse 88.5. Ka lebaka leo, ho hloka batho ba babeli ho e phahamisa le ho e tsamaisa ka mokhoa o sireletsehileng.
Hokela hape lihapo tsa lift ho isa Flex ea hau sebakeng se secha, se haufi. Ho phahamisa le ho jara Flex ka matsoho a eona ke tsela e nepahetseng ea ho tsamaisa roboto sebaka se sekhutšoanyane. Tlosa li-handle 'me u li boloke ho User Kit ka mor'a hore ho falla ho phethoe. Ho thibela ho senya roboto, kamehla sebelisa lithupa tsa lifti ho e nka le ho e tsamaisa. Se ke oa tšoara foreimi ho phahamisa kapa ho tsamaisa roboto ea hau.
Metsamao ya sebaka se selelele
Ho tsamaea sebaka se selelele ho tlosa Flex ea hau sebakeng sa univesithi, setsing kapa setsing sa hau. Ho pholletsa le toropo, ho ea toropong e ncha, setereke, profinse, kapa naha kaofela ke exampbofokoli ba ho tsamaya sebaka se selelele. Tabeng ena, o tla hloka ho paka Flex ho e sireletsa linthong tsa tlhaho, litšisinyeho le metsamao e mpe e ka bang teng ha u le tseleng.
Haeba u bolokile kreiti ea thomello le litšehetso tsa kahare tse tlileng le Flex ea hau, u ka e paka hape ka har'a lisebelisoa tsena bakeng sa ho tsamaea hole. Latela mehato ea unboxing ka tatellano e ka morao ho lokisa Flex ea hau bakeng sa motsamao o molelele. Ha e le hantle, u lokela ho:
Hakolla thapo ya motlakase le ya netweke, haeba e hoketswe. Tlosa lisebelisoa tsohle tse khomaretsoeng le labware. Kenya lipoleiti tsa mokato hape. Notlela seketsoana (sheba karolo ea General Moving Advice ka tlase). Tlosa 'me u boloke liphanele tsa fensetere.
Haeba u bolokile crate ea mantlha:
Hokela hape foreimi ea thomello ho Flex 'me u e sireletse setsing sa pallet u sebelisa li-brackets tsa L. Eketsa padding 'me u kopanye hape lebokose la ho tsamaisa thepa.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
Haeba ha u na krete ea mantlha le lintho tse amanang le tsona, ikopanye le k'hamphani ea likepe e tsebahalang. Ba ka u laola ho paka, ho palangoa le ho u tsamaisa.
Keletso e akaretsang e tsamaeang
KHAOLA LIKHABELLO TSA MATLA LE TSA NETEKANE Pele u tsamaisa Flex ea hau, u se ke ua lebala ho: Tima motlakase 'me u o lokolle phepelong ea motlakase. Hakolla thapo ea Ethernet kapa ea USB, haeba e sebelisoa.
LOCK THE GANTRY Pele o tsamaisa Flex ea hau, kenya hape li-screws tsa ho notlela ho tšoara lekhalo sebakeng. Libaka tsa ho notlela tsa gantry li fumaneha: Ka lehlakoreng le letšehali la terene haufi le ka pele ho roboto. Ka tlas'a letsoho le otlolohileng la gantry. Ka lehlakoreng le letona la terene haufi le ka pele ho roboto. Ho notlela karolo ena ea gantry ho hloka tse nyane
borakete ea lamunu le likurufu tse peli tsa ho notlela.

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KHAOLO EA 2: HO KENYA LE HO FELISOA
HOME THE GANTRY Ho ka etsahala hore ha o batle ho notlela gantry haeba o isa roboto sebakeng se haufi. Haeba u etsa qeto ea ho se e notlele, bonyane sebelisa sekirini sa ho ama kapa Opentrons App ho romela gantry sebakeng sa eona sa lapeng pele o e theola. Ho kenya lekhalo lapeng ka sekirini sa ho thetsa, tlanya menyu ea matheba a mararo () ebe o tlanya Home Gantry. Ho ea lapeng la gantry ka Opentrons App: Tobetsa Lisebelisoa. Tobetsa ho Flex ea hau lethathamong la lisebelisoa. Tobetsa lenane la matheba a mararo () ebe o tobetsa Lehae gantry.
TLOSA LI-MODULES Li-module tse ka har'a deck le lihokelo tse ling li eketsa boima bo eketsehileng ho Flex ea hau. Li boetse li ama setsi sa matla a khoheli sa roboto, se ka etsang hore e ikutloe e le "tippy" ha e e phahamisa. Ho thusa ho bebofatsa le ho leka-lekanya roboto, tlosa lisebelisoa life kapa life tse khomaretsoeng pele u li nka.
REINSTALL DECK SLOTS Re khothaletsa ho kopanya libaka tsa deck bakeng sa ho tsamaea hole. Ho boloka li-slots libakeng tsa bona tsa mantlha ho thusa ho thibela tahlehelo e sa lebelloang. Ho hokela libaka tsa mekato bakeng sa metsamao e khutšoane ho potoloha lab ke boikhethelo.
POST-MOVE RECALIBRATION U lokela ho lokisa lisebelisoa le likarolo life kapa life ka mor'a ho li kenya hape. Bakeng sa lintlha tse ling mabapi le ho lekanya li-module, sheba khaolo ea Li-module.

OPENTRONS FLEX

KHAOLO EA 2: HO KENYA LE HO FELISOA
Mehopolo ea ho qetela mabapi le ho falla
Your Flex ke mochini o tiileng le o hahiloeng hantle, empa hape ke sesebelisoa se nepahetseng sa mahlale se etselitsoeng ho hloka mamello. Ka lebaka leo, u lokela ho e tšoara ka hloko ha u e fallisetsa sebakeng sa heno sa mosebetsi kapa u e romela ho pholletsa le naha. Sena se bolela ho latela tataiso e fanoeng mona le ho sebelisa kelello ea hau e tloaelehileng mabapi le mokhoa oa ho tsamaisa thepa e turang ea lisebelisoa tsa laboratori. Ntlha ea bohlokoa: ha u tsamaisa Flex ea hau, etsa phoso ka lehlakoreng la tlhokomeliso le padding e eketsehileng.
Haeba u na le lipotso kapa matšoenyeho mabapi le ho fallisa Flex ea hau, ikopanye le rona ho support@opentrons.com.

OPENTRONS FLEX

KHAOLO EA 3
Tlhaloso ea Tsamaiso

Khaolo ena e hlalosa lits'ebetso tsa lisebelisoa tsa Opentrons Flex, tse ipapisitseng le likarolo tsa eona tsa mantlha tsa lab. Sebaka sa marang-rang, li-gantry, le lisebelisoa tsa Opentrons Flex li nolofalletsa tšebeliso ea lisebelisoa tse nepahetseng tsa metsi le lisebelisoa tsa ho sebetsana le labware. Sekerine sa ho ama se sesebelisoa se thusa ho tsamaisa liprothokholo le ho sheba boemo ba roboto ntle le ho hloka ho tlisa komporo ea hau bencheng ea lab. Khokahano e nang le mehala le ea waelese e nolofalletsa taolo e eketsehileng ho tsoa ho Opentrons App (sheba khaolo ea Software le Operation bakeng sa lintlha tse ling) le ho atolosa likarolo tsa sistimi ka ho hokela li-peripherals (sheba khaolo ea Li-Modules).

3.1 Likarolo tsa 'mele

Khamera

Leseli la Boemo

Skrine se thetsoang

Foreimi

Deck ea Gantry
Monyako o ka Pele
Libaka tsa likarolo tsa 'mele tsa Opentrons Flex.

Side Windows Handle Caps

OPENTRONS FLEX

KHAOLO EA 3: TLHALOSO EA TSAMAISO

Foreimi le kampetsoeng
Foreimi ea roboto ea Opentrons Flex e fana ka ts'ehetso e tsitsitseng le ea sebopeho bakeng sa mokato oa eona le gantry. Li-subsystem tsohle tsa mochini li lutse holim'a le ho beoa foreiming e kholo. The foreimi e hahiloe haholo-holo ka lakane tšepe le extrusions aluminium.
Foreimi ea tšepe e na le menyetla ea lifensetere tse mahlakoreng le lemati le ka pele le entsoeng ka polycarbonate e bonaletsang e u lumellang hore u bone se etsahalang ka har'a Flex. Lihokelo tsa monyako o ka pele li bulehile bakeng sa ho fihlella kahare ho sistimi. Ha lemati le ka pele le bulehile, o ka hokela lisebelisoa, li-module le li-deck fixtures; lokisetsa mokato pele ho protocol; kapa ho laola boemo ba deske nakong ea protocol.
Lithapa tse tšoeu tsa LED tse ka mathōkong a ka hare a foreimi li fana ka mabone a ambient a laoloang ke software. Khamera ea 2-megapixel e ka nka setšoantšo sa mokatong le sebaka sa ho sebetsa bakeng sa ho rekota le ho latela ts'ebetso ea protocol.

Deck le sebaka sa ho sebetsa
Bokaholimo ke sebaka sa aluminium se entsoeng ka mochini moo liprothokholo tsa mahlale a ikemetseng li etsoang teng. Bokaholimo bo na le li-slots tse 12 tsa mantlha tsa ANSI/SLAS-format tse ka hlophisoang bocha hore li tšoare li-labware, li-module, le lisebelisoa. Li-slots tsa mekato li khetholloa ka sistimi e hokahanyang, e nang le slot A1 ka morao ho le letšehali le slot D3 ka pele ho le letona.

Sebaka sa Katoloso (bakeng sa Thermocycler) Sebaka sa Ts'ebetso

StagSebaka

Libaka tsa mokato ka har'a Flex.

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KHAOLO EA 3: TLHALOSO EA TSAMAISO
Sebaka sa ho sebetsa ke sebaka sa 'mele se ka holim'a mokato o fumanehang bakeng sa pipetting. Lisebelisoa tse behiloeng ka har'a slots A1 ho ea ho D3 li sebakeng sa ho sebetsa.
Opentrons Flex e tla le li-slots tsa mekato e tlosoang bakeng sa maemo ohle a 12 sebakeng sa mosebetsi. Sebaka se seng le se seng sa mekato se na le likotoana tsa labware tsa sekhutlo bakeng sa ho beha li-labware ka mokhoa o sireletsehileng mokatong oa sekepe.
U ka lokisa mokato ka ho fetola li-slots ka lisebelisoa tse ling tsa mekato, ho kenyeletsoa lithōle tse suthang, li-waste chute le li-module caddies. Sebaka sa katoloso se ka morao ho A1 se sebelisoa feela ho etsa sebaka se eketsehileng bakeng sa Thermocycler Module, e lutseng A1 le B1.
Tlhokomeliso: Li-slots tsa Deck li ka fapanyetsanoa ka har'a kholomo (1, 2, kapa 3) empa eseng ka har'a likholomo; kholomo ea 1 le kholomo ea 3 likotoana ke likotoana tse ikhethileng ho sa tsotellehe boholo ba tsona bo tšoanang. U ka tseba hore na sekotjana se kena ho eng ka ho lebisa sekotwana sa labware ka ho le letšehali ka morao.
U lokela ho siea li-slots tse kentsoeng libakeng tseo u batlang ho beha li-labware tse ikemetseng. Sebaka sa marang-rang le lintho tse behiloeng holim'a eona li lula li tsitsitse, ntle le haeba li susumetsoa ke gripper kapa ho kenella ka letsoho.
Stagsebaka
Likarolo tsa staging sebaka ke sebaka se eketsehileng ka lehlakoreng le letona la mokato. U ka boloka labware sebakeng sena ka mor'a ho kenya staglibaka tsa slots. Labware beha ka slots A4 ka D4 ba ka staging area. Flex pipettes e ke ke ea fihla ho staging, empa gripper e ka nka le ho tsamaisa labware ho ea le ho tloha sebakeng sena. Ho kenya li-slots tse ling ho thusa ho boloka sebaka sa ho sebetsa se le teng bakeng sa lisebelisoa tse sebelisoang ho liprothokholo tsa hau tse ikemetseng.
Staglibaka tsa libaka li kenyellelitsoe tlhophisong e itseng ea setsi sa mosebetsi 'me li fumaneha hape bakeng sa ho rekoa ho https://shop.opentrons.com.

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Staging Sebaka se nang le Slots e kentsoeng

Lisebelisoa tsa mekato
Li-fixtures ke lintho tsa Hardware tse nkang sebaka sa li-slots tse tloaelehileng. Ba u lumella hore u iketsetse sebopeho sa mekato le ho eketsa ts'ebetso ho Flex ea hau. Hajoale, lisebelisoa tsa mekato li kenyelletsa staglibaka tse betliloeng, moqomo oa lithōle o ka hare, le moqomo oa litšila o kantle. U ka kenya li-fixtures ka har'a li-slots tse 'maloa tse ikhethileng. Tafole e latelang e thathamisa libaka tsa mokatong o mong le o mong.

Sebopeho sa Staging sebaka slots Lithōle bin Litšila chute Litšila chute le stagsekotjana sa sebaka

Slots A3D3 A1D1 le A3-D3 D3 D3 feela

Lisebelisoa ha li na matla. Ha li na lisebelisoa tsa elektroniki kapa tsa mochini tse fetisang boemo ba tsona ba hajoale le sebaka sa mekato ho roboto. Sena se bolela hore o tlameha ho sebelisa sebopeho sa mekato ho tsebisa Flex hore na ho na le lisebelisoa life tse khomaretsoeng mokatong le moo li leng teng.

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U ka fihlella litlhophiso tsa tlhophiso ea mekato ho tsoa skrineng se ts'oaroang ka li-menu tsa matheba a mararo () le ho tsoa ho Opentrons App. Sheba karolo ea Deck Configuration ea Khaolo ea Software le Ts'ebetso bakeng sa tlhaiso-leseling ea hore na u ka hlophisa mokato joang ho tloha skrineng sa ho ama.

Chelete ea litšila
Opentrons Flex Waste Chute e fetisetsa metsi, malebela, li-racks, le lipoleiti tsa seliba ho tloha ka har'a Flex e kampetsoeng ho ea mokelikeli oa lithōle o behiloeng ka tlas'a ho buloa ha oona ka ntle. Lesela la litšila le hokela ho adaptara ea mekato e lekanang sekotjana sa D3. E boetse e tla le fensetere e khethehileng ea halofo ea phanele e lumellang chute hore e atolose ho tsoa ka pele ho roboto.
Likarolo tsa chute ea litšila.

Koahela Deck Plate Adapter

Hlakola Chute
Deck Plate Adapter e nang le StagSebaka

Staglibaka tsa slots
Staging area slots ke likotoana tsa ANSI/SLAS tse lumellanang tse nkang sebaka sa maemo a tloaelehileng kholomong ea 3 le ho eketsa libaka tse ncha ho s.taging area - bohle ntle le ho lahleheloa ke sebaka sebakeng sa ho sebetsa. U ka kenya slot e le 'ngoe kapa boholo ba li-slots tse' ne ho theha kholomo e ncha (A4 ho ea ho D4) ka lehlakoreng le letona la mokatong. Leha ho le joalo, hlokomela hore ho nkela sebaka sa A3 sebaka sa deck ho hloka ho tsamaisa moqomo oa lithōle. Ka ho eketsa staging sebaka slots ho mokatong oa, roboto ea hau ea Flex e ka boloka li-labware tse ngata le ho sebetsa ka mokhoa o atlehileng haholoanyane.

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Flex stagsekotjana sa sebaka.
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KHAOLO EA 3: TLHALOSO EA TSAMAISO
KEKETSO SEKOTI
Ho kenya, tlosa li-screws tse hokelang sekotjana se tloaelehileng mokatong 'me u se nkele sebaka ka stagsekotjana sa sebaka. Ka mor'a ho kenya, sebelisa "touchscreen" kapa Opentrons App ho bolella roboto eo u e kentseng joalo ka eonatagsekotjana sa sebaka ho ea mokatong.
Ho kenya e letagsekotjana sa sebaka.

KOPANO Slot Staging sebaka slots e lumellana le lisebelisoa tsa Flex, li-module, le labware tse thathamisitsoeng ka tlase.

Flex karolo ea Gripper Pipettes Modules
Lesebelisoa

Stagho tsamaellana ha sebaka
Flex Gripper e ka tsamaisa labware ho ea kapa ho tloha staglibaka tsa slots.
Flex pipettes e ke ke ea fihla ho staging area. Sebelisa gripper ho tsamaisa likeletso liraka le labware ho tloha stagho kenya sebaka sebakeng sa mosebetsi pele ho phala.
Magnetic Block GEN1 e ka behoa kholomong ea 3 ka holim'a etagsekotjana sa sebaka. Li-module ha li tšehetsoe kholomong ea 4.
Li-module tsa motlakase tse kang Heater-Shaker le Temperature Module li kena ka har'a li-caddy tse ka kenngoa kholomong ea 3. U ke ke ua eketsataging sebaka sekotjana ho boemo ba mojule caddy.
Staglibaka tsa ing li na le litekanyo tse tšoanang tsa ANSI/SLAS joalo ka mekotla e tloaelehileng ea mokato. Sebelisa labware e lumellanang le gripper ho stagsebakeng sena, kapa ka letsoho eketsa le ho tlosa labware sebakeng sena.

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Tsamaiso ea motsamao
Ho khomaretsoe foreiming ho na le gantry, e leng mokhoa oa ho tsamaea le boemo ba roboto. The gantry e tsamaea ka ho arohana x- le y-axis ho beha pipette le gripper libakeng tse nepahetseng bakeng sa ho phethahatsa protocol. Ho tsamaisana le lilepe tsena ho nepahetse ho isa ho 0.1 mm e haufi. The gantry e laoloa ke 36 VDC hybrid bipolar stepper motors. Ka lehlakoreng le leng, ho kenyelelitsoe ho gantry ke li-pipette mounts le sebaka sa ho atolosa. Tsena li tsamaea haufi le z-axis ho beha li-pipette le gripper libakeng tse nepahetseng bakeng sa ts'ebetso ea protocol. Movement hammoho le axis ena e laoloa ke 36 VDC hybrid bipolar stepper motors. Lisebelisoa tsa elektronike tse ka har'a gantry li fana ka matla a 36 VDC le puisano ho li-pipette le gripper, ha li khomaretsoe.
Gantry

Lithaba tsa Pipette

Extension Mount

Sebaka sa lisebelisoa tsa lisebelisoa ho Flex.

Lits'oants'o tsa skrini le lisebelisoa tsa LED
Sebopeho sa mantlha sa mosebelisi ke sekirini sa LCD sa 7-inch, se ka pele ho le letona la roboto. The touchscreen e koahetsoe ka Gorilla Glass 3 bakeng sa ho ingoaea le ho hanyetsa tšenyo. Fumana likarolo tse ngata tsa Flex hantle skrineng sa ho ama, ho kenyelletsa:

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Tsamaiso ea protokhole Ho seta, ho etsa, le ho beha leihlo Tsamaiso ea Labware Litlhophiso tsa Robot Software ea sistimi le liapdeite tsa firmware Li-log tsa ts'ebetso le litsebiso tsa liphoso.
Bakeng sa tlhaiso-leseling e batsi mabapi le ho sebelisa Flex ka sekirini sa ho ama, bona karolo ea Ts'ebetso ea Touchscreen ea khaolo ea Software le Operation.
Leseli la boemo ke lesela la li-LED le kaholimo ho roboto le fanang ka tlhaiso-leseling ka roboto. Mebala le lipaterone tse fapaneng tsa khanya li ka bua ka katleho e fapaneng, ho hloleha kapa maemo a sa sebetseng:

Mmala oa LED White Neutral states
Green Normal States
Blue Mandatory e re Yellow Abnormal States Red Emergency states

LED paterone Solid Pulsing
E panya habeli
Pulsing e tiileng

Boemo ba roboto
E butse 'me ha e sebelise protocol Robot e phathahane (mohlala, ho ntlafatsa software kapa firmware, ho theha protocol run, canceling protocol run) Ketso e phethiloe (mohlala, protocol e bolokiloeng, software e nchafalitsoe, sesebelisoa se khomaretsoeng kapa se koaletsoe) Protocol e ntse e sebetsa. Protocol e emisitsoe

Tiileng

Phoso ea software

E panya ka makhetlo a mararo, khafetsa

Phoso ea 'mele (mohlala, ho oa ha sesebelisoa)

Leseli la boemo le ka boela la koaloa litlhophisong tsa roboto.

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3.2 Pipettes
Li-pipette tsa Opentrons ke lisebelisoa tse ka lokisoang tse sebelisoang ho tsamaisa maro sebakeng sa ts'ebetso nakong ea ts'ebetso ea liprothokholo. Ho na le lipeipi tse 'maloa tsa Opentrons Flex, tse khonang ho sebetsana le li-volumes ho tloha ho 1 µL ho isa ho 1000 µL ho li-channel tse 1, 8, kapa tse 96:
Opentrons Flex 1-Channel Pipette (1 µL) Opentrons Flex 50-Channel Pipette (1 µL) Opentrons Flex 5-Channel Pipette (1000 µL) Opentrons Flex 8-Channel Pipette (1 µL) Opentrons 50 Flex 8 µL) Opentrons 5 Flex 1000
Li-pipettes li hokela ho gantry li sebelisa li-screws tse hapuoeng ka pele ho pipette. 1-channel le 8-channel pipette e 'ngoe le e' ngoe e lula holim'a pipette e le 'ngoe (ka ho le letšehali kapa ka ho le letona); pipette ea 96-channel e nka lithabeng ka bobeli. Bakeng sa lintlha tse mabapi le ho kenya li-pipette, bona Ho kenyeletsoa ha Instrument le Calibration.

Tlamahaneloa
Likokoana-hloko

Li-Screw Attachment Screw

Ejector

Nozzles (Replaceable O-rings)

Nozzles (Fixed O-rings)

Libaka tsa likarolo tsa 1-, 8-, le 96-channel pipettes.

Ejector

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Li-pipette li nka malebela a polasetiki a lahloang ka ho li hatella holim'a li-nozzles tsa pipette, ebe li sebelisa malebela ho lakatsa le ho fana ka metsi. Palo ea matla a hlokahalang bakeng sa ho lata e ea eketseha ha malebela a mangata a nkuoa ka nako e le 'ngoe. Bakeng sa lipalo tse nyane tsa malebela, pipette e hokela malebela ka ho sutumelletsa molomo o mong le o mong oa pipette tlase ntlheng. Ho fihlela matla a hlokahalang a ho nka rack e felletseng ea malebela, pipette ea 96-channel e boetse e hula malebela holimo holim'a li-nozzles. Ketso ena ea ho hula e hloka hore ho behoe li-racks ka har'a adaptara ea "tip rack", ho e-na le ka ho toba sekoting sa mekato. Ho lahla malebela (kapa ho a khutlisetsa rakeng ea bona), mochini oa ejector oa pipette o sutumelletsa malebela ho tsoa ho li-nozzles.

Litlhaloso tsa Pipette
Opentrons Flex pipettes e etselitsoe ho sebetsana le mefuta e mengata e fapaneng. Ka lebaka la boholo ba bona bo pharaletseng, ba ka sebelisa malebela a boholo bo fapaneng, a amang litšobotsi tsa bona tsa ho sebetsana le metsi. Opentrons e lekile li-pipette tsa Flex bakeng sa ho nepahala le ho nepahala ho kopantseng lintlha tse ngata le molumo oa metsi:

Pipette
Flex 1-Channel
50µL
Flex 1-Channel
1000µL
Flex 8-Channel
50µL
Flex 8-Channel
1000µL

Keletso Bokhoni 50 µL 50 µL 50 µL 50 µL 50 µL 200 µL 1000 µL 50 µL 50 µL 50 µL 50 µL 50 µL 200L 1000

E Lekoa Moqolo oa 1 µL 10 µL 50 µL 5 µL 50 µL
200 μL 1000 μL
1 µL 10 µL 50 µL 5 µL 50 µL 200 µL 1000 µL

Nepahalo %D 8.00% 1.50% 1.25% 5.00% 0.50% 0.50% 0.50% 10.00% 2.50% 1.25% 8.00% 2.50% 1.00% 0.70%

Precision %CV 7.00% 0.50% 0.40% 2.50% 0.30% 0.15% 0.15% 8.00% 1.00% 0.60% 4.00% 0.60% 0.25% 0.15%

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Flex 96-Channel
1000µL

50 μL 50 µL 200 µL 1000 µL

5 μL 50 µL 200 µL 1000 µL

10.00% 2.50% 1.50% 1.50%

5.00% 1.25% 1.25% 1.50%

Hopola lintlha tsena tse nepahetseng ha u khetha malebela bakeng sa pipette ea hau. Ka kakaretso, bakeng sa liphetho tse ntle u lokela ho sebelisa malebela a manyane a fihlelang litlhoko tsa protocol ea hau.

Tlhokomeliso: Opentrons e etsa tlhahlobo ea volumetric ea li-pipette tsa Flex ho etsa bonnete ba hore li kopana le lintlha tse nepahetseng le tse nepahetseng tse thathamisitsoeng ka holimo. Ha ua tlameha ho lekanya molumo oo li-pipette tsa hau li o ntšang pele u sebelisoa. U tlameha feela ho etsa calibration ea boemo. Sheba karolo e latelang, hammoho le karolo ea Pipette Installation ea khaolo ea ho kenya le ho falla, bakeng sa lintlha.
Litšebeletso tsa Opentrons Care le Opentrons Care Plus li kenyelletsa phetoho ea selemo le selemo ea pipette le litifikeiti tsa ho lekanya. Sheba karolo ea Servicing Flex ea khaolo ea Maintenance and Service bakeng sa lintlha tse ling.

Pipette calibration
The User Kit e kenyeletsa tšepe ea ho lekanya pipette, eo u e sebelisang nakong ea ho lekanya boemo. Nakong ea ts'ebetso ea protocol, boloka ka mokhoa o sireletsehileng probe ho sets'oants'o sa makenete holim'a pilara e ka pele ea roboto. Nakong ea ts'ebetso ea ho lekanya, hokela probe ho nozzle e loketseng ebe u e notlela sebakeng. Roboto e suthisa probe libakeng tsa ho lekanya setulong ho metha moo pipette e leng teng.
Adapter ea pipette tip rack
The Opentrons Flex 96-channel pipette likepe tse nang le li-adapter tse 'nè tsa rack. Tsena ke li-brackets tsa aluminium tse entsoeng ka nepo tseo u li behang mokatong. Li-adapter li na le li-racks tsa Flex 50 L, 200 L le 1000 μL.

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Ka lebaka la matla a amehang, pipette ea 96-channel e hloka adaptara ho hokela rack e felletseng hantle. Nakong ea ts'ebetso ea ho kopanya, pipette e tsamaea holim'a adaptara, e theohela holim'a lithakhisa tse ntseng li kenngoa, ebe e hula malebela holim'a li-pipette ka ho phahamisa adaptara le rack ea ntlha. Ho hula malebela, ho fapana le ho sutumetsa, ho fana ka matla a hlokahalang ho boloka malebela ho li-pipette le ho thibela ho sotha bokaholimo ba mokatong. Ha e felile, pipette ea 96-channel e theola adaptara le rack e se nang letho holim'a mokato. Sheba karolo ea Malebela le malebela a khaolo ea Labware bakeng sa tlhaiso-leseling e batsi.
Ho lata likeletso tse sa fellang
Pipette ea 96-channel e ka nka rack e feletseng ea malebela kapa palo e nyenyane ea malebela. Sena se eketsa palo ea lits'ebetso tseo u ka li etsang ka pipette ea li-channel tse 96, kaha e na le li-pipette tse peli.
Hajoale, pipette ea li-channel tse 96 e ts'ehetsa ho nka malebela a 8 ka sebopeho sa kholomo. Tokisong ena, pipette e sebelisa li-nozzles tsa eona tse ka ho le letšehali ho nka malebela ho tloha ho le letona ho ea ho le letšehali ho tloha ho rack, kapa li-nozzles tsa eona tse ka ho le letona ho nka malebela ho tloha ho le letšehali ho ea ho le letona ho tloha rack.
Ha u nka malebela a ka tlase ho 96 ho tloha rack, rack e tlameha ho beoa ka kotloloho mokatong, eseng ka har'a adaptara ea rack.
Li-sensor tsa Pipette
Li-pipette tsa Opentrons Flex li na le li-sensor tse ngata tse lemohang le ho rekota lintlha tse mabapi le boemo ba pipette le malebela leha e le afe ao e a nkileng.
MOKHOA MATSOSO
Ho kopantsoe le probe ea tšepe kapa ntlha e tsamaisang, li-sensor tsa capacitance li lemoha ha pipette e kopana le ntho e itseng. Ho lemoha ho kopana pakeng tsa probe ea tšepe le mokatong o sebelisoa ka mokhoa o ikemetseng oa ho lekanya pipette le mekhoa ea ho lekanya li-module.
Li-pipette tsa 1-channel li na le sensor e le 'ngoe ea capacitance, ha li-pipette tse ngata li na le tse peli: li-channel 1 le 8 tsa li-pipette tsa 8, le li-channel 1 le 96 (maemo a A1 le H12) a pipette ea 96-channel.

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TŠEBELETSO PELE HO HLAHALA
Sesepa sa photointerruptor se lemoha boemo ba mochine oa ejector oa pipette, ho tiisa hore na malebela a ile a nkoa ka katleho kapa a lihuoa. 1-channel, 8-channel, le 96-channel pipettes kaofela li na le sensara e le 'ngoe ea optical e hlokomelang sehokelo sa likeletso ho likanale tsohle.
Lintlafatso tsa firmware ea Pipette
Opentrons Flex e ntlafatsa firmware ea pipette ka bo eona ho e boloka e hokahane le mofuta oa software ea liroboto. Lintlafatso tsa firmware tsa Pipette hangata li potlakile, 'me li etsahala neng kapa neng ha:
U kopanya pipette. Roboto e qala hape.
Haeba, ka lebaka lefe kapa lefe, liphetolelo tsa hau tsa firmware le liroboto li tsoa ho sync, u ka khona ho nchafatsa firmware ho Opentrons App.
1. Tobetsa Lisebelisoa. 2. Tobetsa ho Flex ea hau lethathamong la lisebelisoa. 3. Tlas'a Lisebelisoa le Li-Module, pipette e sa sebetseng e tla bontša ho bala banner ea temoso
"Firmware ea ntlafatso e teng." Tobetsa Update hona joale ho qala ntjhafatso.
U ka khona view mofuta oa firmware o kentsoeng hajoale oa pipette efe kapa efe e khomaretsoeng. Ka skrineng ea ho ama, e ea ho Lisebelisoa ebe u tlanya lebitso la pipette. Ho Opentrons App, fumana karete ea pipette tlas'a Instruments and Modules, tobetsa menu ea matheba a mararo (), ebe u tobetsa About pipette.

3.3 Gripper
The gripper susumelletsa labware ho pholletsa le sebaka sa ho sebetsa le stagsebaka sa ho sebetsa nakong ea ho etsoa ha liprothokholo. The gripper e khomarela thaba e atolositsoeng, e arohaneng le lithapo tsa pipette; gripper e ka sebelisoa ka tlhophiso efe kapa efe ea pipette. Bakeng sa lintlha tse mabapi le ho kenya gripper, bona ho Kenyeletsoa ha Instrument le Calibration.
Sebapali se ka tsamaisa labware ho pholletsa le mokato le ho kena kapa ho tima li-module. Sebapali se khona ho qhekella lipoleiti tsa seliba tse koetsoeng ka botlalo, lipoleiti tsa liliba tse tebileng le li-racks. Bakeng sa lintlha tse ling mabapi le hore na gripper e ka tsamaisa lisebelisoa life, bona karolo ea Labware le Opentrons Flex Gripper khaolong ea Labware, kapa sheba Laeborari ea Opentrons Labware.

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Litlhaloso tsa Gripper
Mehlahare e etsa motsamao o ka sehloohong oa gripper, e leng ho bula kapa ho koala li-paddles tse peli tse bapileng ho sebelisa kapa ho lokolla matla mahlakoreng a labware. Motsamao oa mehlahare o laoloa ke 36 VDC brushed motor e hokahantsoeng le rack-and-pinion gear system.
Ho tsamaisa sengoathoana sa labware se ts'oeroeng ke mehlahare, gantry e phahamisa gripper haufi le z-axis, e e tsamaisa ka thōko, ebe e e theolela sebakeng se secha sa labware.
Libaka tsa likarolo tsa gripper.

Li-screws tse hokelang
Calibration Pin Jaws Paddles

Gripper calibration
The gripper e kenyelletsa phini ea tšepe ea calibration. Pini ea calibration e sebakeng sa polokelo e khutsitsoeng karolong e ka tlase ea gripper. Makenete o tšoara phini sebakeng sa eona. Ho tlosa phini ea calibration, e tšoare ka menoana ea hau ebe u e hula ka bonolo. Ho khutlisa phini, e khutlisetse sebakeng sa polokelo. U tla tseba hore e bolokehile ha e fihla sebakeng sa eona.
Ha u etsa calibrating gripper, hokela pinana mohlahareng o mong le o mong ka ho latellana. Roboto e suthisa phini libakeng tsa ho lekanya holim'a deki ho metha moo gripper e leng teng.
Nakong ea ts'ebetso ea protocol, beha pinana sebakeng sa eona sa polokelo hore se bolokehe. Ikopanye le rona ho support@opentrons.com haeba o lahleheloa ke pin ea calibration.

Lintlafatso tsa firmware ea Gripper
Opentrons Flex e nchafatsa firmware ea gripper ka bo eona ho e boloka e hokahane le mofuta oa software ea liroboto. Lintlafatso tsa firmware ea Gripper hangata li potlakile, 'me li etsahala neng kapa neng ha:

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U hokela gripper. Roboto e qala hape.
Haeba, ka lebaka lefe kapa lefe, liphetolelo tsa hau tsa gripper firmware le liroboto li tsoa ho sync, u ka khona ho nchafatsa firmware ho Opentrons App.
1. Tobetsa Lisebelisoa. 2. Tobetsa ho Flex ea hau lethathamong la lisebelisoa. 3. Tlas'a Lisebelisoa le Li-Modules, gripper e sa sebetseng e tla bonts'a ho bala banner ea temoso.
"Firmware ea ntlafatso e teng." Tobetsa Update hona joale ho qala ntjhafatso.
U ka khona view mofuta oa firmware o kentsoeng hajoale oa gripper. Ho skrine sa ho ama, e ea ho Lisebelisoa ebe u tlanya gripper. Ho Opentrons App, fumana karete ea gripper tlas'a Instruments and Modules, tobetsa menu ea matheba a mararo (), ebe u tobetsa About gripper.
3.4 Pendanta ea Emergency Stop
Emergency Stop Pendant (E-stop) ke konopo ea hardware e ikemiselitseng ho emisa ho sisinyeha ha roboto kapele. Opentrons Flex e hloka E-stop e sebetsang, e sa sebetseng hore e hoketsoe ka linako tsohle. Ha o tobetsa konopo ea ho emisa, Flex e hlakola protocol efe kapa efe e sebetsang kapa setup workflow kapele kamoo ho ka khonehang mme e thibela ho sisinyeha ha liroboto tse ngata.
Nako ea ho sebelisa E-stop
U ka 'na ua hloka ho tobetsa E-stop:
Ha ho na le kotsi e haufi ea ho tsoa kotsi kapa kotsi ho mosebelisi. Ha ho na le kotsi e haufi ea ho senya roboto kapa lisebelisoa tse ling. Ha sampli-reagents li kotsing e haufi ea ho tšoaetsoa. Ka mor'a ho thulana ha hardware.
Hantle-ntle ha ua lokela ho tobetsa E-stop (ntle le nakong ea tlhahlobo ea boleng ba hardware e sa tloaelehang).
Se ke oa sebelisa E-stop ho hlakola lits'ebetso tse tloaelehileng, tse lebelletsoeng. Ho e-na le hoo, sebelisa konopo ea software skrineng sa ho ama kapa ho Opentrons App. Ho khefutsa ka software ho tla u lumella ho qala kapa ho hlakola protocol ea hau, athe ho tobetsa E-stop kamehla ho hlakola protocol hang hang.

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Ho kenya letsoho le ho lokolla E-stop
E-stop e na le mochine oa ho tobetsa-to-engage, twist-to-release mechanism.
Kenyelletsa: Tobetsa fatše ka tieo holim'a konopo e khubelu. Flex e tla kena sebakeng se emisitsoeng. Rarolla: Hang ha e emisitsoe, rarolla mathata leha e le afe sebakeng sa ts'ebetso ka mokhoa o sireletsehileng, joalo ka ho tlosa litšila,
ho tlosa labware, kapa ho tsamaisa gantry (e lokela ho tsamaea ka bolokolohi le habonolo ka letsoho). Phatlalatso: Sotha konopo ho ea ka nako. E tla hlahella sebakeng sa eona se sa sebetseng. Seta bocha: Ho skrineng sa ho ama kapa ho Opentrons App, netefatsa hore u se u loketse hore Flex e qale hape
tshisinyo. Gantry e tla khutlela sebakeng sa eona sa lapeng mme mosebetsi oa module o tla qala hape.
Boemong bo emisitsoeng, Flex le lisebelisoa tse hokahaneng li tla sebetsa ka tsela e latelang:

Hardware Gantry Pipettes
Gripper
Heater-Shaker Module
Temperature Module Thermocycler Module Status lightscreen Touchscreen

Boitšoaro
Motsamao o otlolohileng o emisitsoe. Motsamao o otlolohileng ka letsoho o lumelletsoe.
Motsamao o otlolohileng oa li-pipette o emisitsoe. mariki a enjene holim'a lilepe tse otlolohileng lia sebetsa ho thibela
pipettes ho tloha ho oela. Motsamao oa plunger le ho lata lintlha li emisitsoe.
Motsamao o otlolohileng oa gripper o emisitsoe. Brake ea enjene holim'a axis e emeng e tšoaretsoe ho thibela
mokgoaretsi ho tloha ho wela. Li-motors tsa mohlahare tse sebelisang matla a ho tšoara li lula li le teng, kahoo
gripper e ke ke ea liha labware eo e ka 'nang ea e jara.
The shaker emisa le mahae. Latch ea labware ea buleha. Ho futhumatsa ho koetsoe.
Ho futhumatsa kapa ho pholisa ho koetsoe.
Ho futhumatsa kapa ho pholisa ho koetsoe.
Leseli le fetoha le lefubelu.
Molaetsa oa ho hlakola o nka skrineng. Letshwao le hlahang skrineng le bontsha ha o atlehile
hlakola konopo ea ho emisa.

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KHAOLO EA 3: TLHALOSO EA TSAMAISO
3.5 Lihokelo

On/Off Switch

Likoahelo Tse Lehlakoreng

Li-Ports tsa USB-A

IEC Power Inlet

Maemakepe AUX-1, AUX-2, USB-B, Ethernet

Khokahano ea matla
Opentrons Flex e hokahana le mohloli oa matla ka seketsoana se tloaelehileng sa IEC-C14. Roboto e na le matla a kahare a marang-rang a AC / DC a felletseng, a amohelang 100 VAC, 240/50 Hz ho kenya le ho e fetolela ho 60 VDC. Lisebelisoa tse ling tsohle tsa ka hare tsa elektroniki li tsamaisoa ke 36 VDC phepelo.

Tlhokomeliso: Sebelisa feela thapo ea motlakase e fanoeng le roboto. Se ke oa sebelisa thapo ea motlakase e nang le motlakase o sa lekaneng kapa voltage litekanyetso.
Boloka thapo ea motlakase e se na litšitiso e le hore u ka e tlosa ha ho hlokahala.

Ho boetse ho na le betri ea sele ea tšepe ea CR1220 ho matlafatsa oache ea nako ea sebele ea roboto ha e sa hokahanngoa le matla a motlakase. Betri e fumaneha ka har'a sekoahelo sa "touchscreen". Ikopanye le Opentrons Support bakeng sa tlhaiso-leseling e batsi haeba o nahana hore o hloka ho khutlisa betri.

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KHAOLO EA 3: TLHALOSO EA TSAMAISO
USB le likhokahano tse thusang
Opentrons Flex e na le likou tsa USB tse 10 tse fumanehang libakeng tse fapaneng tsa roboto, tse sebeletsang merero e fapaneng.
Likou tse 8 tse ka morao tsa USB-A (tse nang le linomoro tsa USB-1 ka USB-8) le likou tse 2 tse thusang (lihokelo tsa M12 tse nang le linomoro tsa AUX-1 le AUX-2) ke tsa ho hokahanya li-module tsa Opentrons le lisebelisoa. Sheba khaolo ea Li-Modules ho fumana lintlha tse ling mabapi le ho hokela lisebelisoa tsena le ho li sebelisa liprothokholeng tsa hau. Boema-kepe bo ka morao ba USB-B ke ba ho hokela roboto ho laptop kapa komporo ea komporo, ho theha puisano le Opentrons App e sebetsang komporong e hoketsoeng. Boema-kepe bo ka pele ba USB-A (USB-9), bo ka tlase ho sekirini sa touchscreen, bo na le ts'ebetso e ts'oanang le ea likou tsa USB-A tse ka morao.
Tlhokomeliso: Likou tsa USB li na le matla a fokolang ho sireletsa roboto le lisebelisoa tse hokahaneng. Phano ea motlakase e arotsoe ka hare ho lihlopha tse tharo tsa likou: likou tsa USB-A tse ka morao (USB-1 ho ea ho USB-4), likou tsa ka morao tsa USB-A (USB-5 ka USB-8), le USB-A e ka pele. boemakepe. E 'ngoe le e 'ngoe ea lihlopha tsena e tla fana ka boholo ba 500 mA ho lisebelisoa tse lumellanang tsa USB 2.0.
Likhokahano tsa marang-rang
Opentrons Flex e ka hokela marang-rang a sebakeng sa heno ka khokahanyo e nang le mehala (Ethernet) kapa e se nang mohala (Wi-Fi).
Boema-kepe ba Ethernet bo ka morao ho roboto. E hokahane le Ethernet hub kapa bulela netweke ea hau. Kapa, ho qala ka mofuta oa 7.1.0 oa liroboto, e hokela ka kotloloho boema-kepeng ba Ethernet komporong ea hau. Mojule oa ka hare oa Wi-Fi o ts'ehetsa marang-rang a 802.11 ac/a/b/g/n a nang le lenakana la 2.4/5 GHz.

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KHAOLO EA 3: TLHALOSO EA TSAMAISO

3.6 Litlhaloso tsa tsamaiso

Litlhaloso tse akaretsang

Litekanyo Weight Deck slots
Skrine se thetsoang
Wi-Fi Ethernet USB
Kenyelletso ea matla a khamera ea Robot
Lisebelisoa phepelo voltage fluctuation Mains phepelo frequency fluctuation Sistimi ea kabo ea phepelo e khuts'oane ea potoloho ea hajoale Sebopeho sa Fensetere Litlhoko tsa moea

87 × 69 × 84 cm / 34.25 × 27 × 33 ho (W, D, H)
88.5 kg / 195 lb 12 ANSI / SLAS-slots e lumellanang sebakeng sa ho sebetsa
(e fumaneha ho li-pipette) 4 slots e eketsehileng bakeng sa staging malebela le labware
(e fumaneha feela ho gripper) 7-inch LCD touchscreen e nang le mengoapo le Gorilla Glass 3 e sa senyeheng.
802.11 ac/a/b/g/n dual-band (2.4/5 GHz)
100 Mbps 9 USB-A boema-kepe bo 1 boema-kepe ba USB-B lebelo la 2.0
2MP, senepe le video 100 VAC, 240 Hz, 50 60 A/1 VAC, 4.0 A/115 VAC
±10%
±5%
TN-S
6.3 A
Moralo oa tšepe o thata le CNC aluminium
Lifensetere tsa mahlakoreng a polycarbonate tse ka tlosoang le lemati le ka pele Bonyane 20 cm / 8 lipakeng tsa yuniti le lebota

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KHAOLO EA 3: TLHALOSO EA TSAMAISO

Litlhoko tsa PC tse hokahaneng

Opentrons App e sebetsa ka: Windows 10 kapa hamorao macOS 10.10 kapa hamorao Ubuntu 12.04 kapa hamorao

Litlhaloso tsa tikoloho

Maemo a tikoloho Thempereichara ea tikoloho Mongobo o amanang le tšilafalo Tekanyo ea tšilafalo

Tšebeliso ea ka tlung feela +20 ho +25 °C (e khothalelitsoe) 40%, e sa koaheleng (e khothaletsoa) 60 (tšilafalo e sa tsamaisaneng feela)

Bakeng sa tlhahisoleseding e eketsehileng mabapi le maemo a amohelehang a tikoloho bakeng sa tšebeliso le lipalangoang, bona karolo ea Maemo a Tikoloho khaolong ea Ho Kenya le Phalliso.

Setifikeiti

Setifikeiti se phethiloe Ha se netefalitsoe/se netefalitsoe

CE, ETL, FCC, ISO 9001 IVD, GMP

Kakaretso ea tlhaiso-leseling ea setifikeiti e hatisitsoe setikarang se ka morao ho Flex, haufi le switch ea on/off. Bakeng sa lintlha tse felletseng tsa setifikeiti le tlhahisoleseling, sheba karolo ea Regulatory Compliance ho Selelekela.

Nomoro ea letoto
Flex e 'ngoe le e' ngoe e na le nomoro ea serial e ikhethang. Sebopeho sa nomoro ea serial se fana ka lintlha tse ling, ho kenyelletsa le letsatsi la tlhahiso ea roboto. Bakeng sa mohlalaampLe, nomoro ea serial FLXA1020231007001 e ka bontša:

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KHAOLO EA 3: TLHALOSO EA TSAMAISO

Litlhaku FLX A10 2023 10 07 001

Sehlopha sa Letsatsi la Letsatsi la Khoeli ea Selemo sa Model Version Unit

Ho bolela Roboto ke Opentrons Flex. Khoutu ea mofuta oa tlhahiso ea roboto. Roboto e entsoe ka 2023. Roboto e entsoe ka Mphalane. Roboto e entsoe ka letsatsi la 7 la khoeli. Nomoro e ikhethang ea liroboto tse entsoeng ka letsatsi le itseng.

U ka fumana nomoro ea serial bakeng sa Flex ea hau:
Setifikeiting sa setifikeiti se ka morao ho Flex, haufi le sesebelisoa sa ho tima/ho tima. Ka lehlakoreng le ka morao la "touchscreen" (ho leba sebakeng sa ho sebetsa). Ho Opentrons App tlas'a Lisebelisoa> Flex ea hau> Litlhophiso tsa Robot> E tsoetseng pele.

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KHAOLO EA 4
Limojule
Opentrons Flex e kopanya le li-module tse ngata tsa Opentrons hardware. Li-module kaofela ke li-peripherals tse lutseng li-slots, 'me boholo ba tsona li laoloa ke roboto holim'a khokahanyo ea USB.
Khaolo ena e hlalosa mesebetsi le litlhaloso tsa 'mele tsa li-module tse lumellanang le tsamaiso ea Opentrons Flex, hammoho le mokhoa oa ho li kopanya le ho li lekanya. Bakeng sa lintlha tse ling mabapi le ho seta le ho sebelisoa ha module, sheba bukana ea litaelo bakeng sa li-module ka bomong. Bakeng sa lintlha tse mabapi le ho kenyelletsa li-module ho liprothokholo tsa hau, bona karolo ea Moqapi oa Prothokhole khaolong ea ntlafatso ea Protocol kapa litokomane tsa inthaneteng tsa Python Protocol API.
4.1 Li-module tse tšehelitsoeng
Opentrons Flex e tsamaisana le mefuta e mene ea li-module tsa Opentrons tse holim'a mokato:
Heater-Shaker Module e fana ka ho futhumatsa ka mokatong le ho sisinyeha ha orbital. Mojule o ka futhumatsoa ho 95 ° C, 'me o khona ho sisinyeha sampho tloha ho 200 ho isa ho 3000 rpm.
Magnetic Block ke sesebelisoa se sa sebetseng se ts'oereng labware haufi le limakenete tsa eona tse matla haholo tsa neodymium. OT-2 Magnetic Module GEN1 le GEN2, tse tsamaisang limakete tsa tsona holimo le tlase tse amanang le labware, ha li tšehetsoe ho Opentrons Flex.
Temperature Module ke mojule oa poleiti e chesang le e batang e khonang ho boloka mocheso o tsitsitseng o pakeng tsa 4 le 95 °C.
Thermocycler Module e fana ka on-deck, thermocycling e feletseng, e nolofalletsang ho iketsetsa mehato e holimo le e tlaase ea ho phalla ha mosebetsi. Thermocycler GEN2 e lumellana ka botlalo le gripper. Thermocycler GEN1 e ke ke ea sebelisoa le gripper, ka hona ha e tšehetsoe ho Opentrons Flex.
Li-module tse ling tse etselitsoeng OT-2 qalong li lumellana le Flex, joalo ka ha li akaretsoa tafoleng e ka tlase. Letšoao la ho hlahloba le bontša ho lumellana, 'me X e bontša ho se lumellane.

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KHAOLO EA 4: LIMOLAO

Mofuta oa sesebelisoa le moloko oa Heater-Shaker Module GEN1 Magnetic Module GEN1 Magnetic Module GEN2 Magnetic Block GEN1 Temperature Module GEN1 Thermocycler Module GEN2 Thermocycler Module GEN1 HEPA Module

OT-2

Flex

4.2 Sistimi ea caddy ea mojule
Li-module tse lumellanang li etselitsoe hore li kenelle ka har'a li-caddy tse lulang sebakeng se ka tlase ho mokato. Sistimi ena e lumella li-labware ka holim'a li-module hore li lule li le haufi le bokaholimo ba mokatong, hape e lumella hore ho be le routing ea cable e ka tlase ho mokatong o ka tlase e le hore mokato o lule o le makhethe ha protocol ea hau e ntse e sebetsa.

Li-Caddies tsa Heater-Shaker, Mocheso, le Thermocycler Modules.

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KHAOLO EA 4: LIMOLAO
Ho kenya mojule ka har'a bokaholimo ba mokato, o tlameha ho qala ka ho beoa ka har'a caddy ea mojule e tsamaellanang. Mofuta o mong le o mong oa mojule o tsamaellanang o na le moralo oa ona oa caddy o hokahanyang module le labware hantle le mokato o o potileng. (Mokhelo ke Magnetic Block, e sa hlokeng matla kapa thapo ea USB routing 'me kahoo e lutse ka ho toba holim'a mokatong oa sekepe.) Li-caddies tsa li-module tse nang le slot e le' ngoe li ka behoa kae kapa kae ho kholomo ea 1 kapa ea 3; Thermocycler e ka behoa feela ka har'a slots A1 le B1 ka nako e le 'ngoe.
Ka kakaretso, ho kenya module caddy:
1. Tlosa li-slots tsa deck sebakeng seo module e tla ea ho sona. 2. Beha module ka har'a caddy ea eona 'me u tiise liankora tsa eona. 3. Tsamaisa matla a module le lithapo tsa USB ka likoahelo tse mahlakoreng, ho ea holimo ka sekoting se se nang letho, le
li hokela mojuleng. 4. Beha caddy ea module ka har'a sekotjana ebe u e fafatsa sebakeng sa eona.
Bakeng sa litaelo tse nepahetseng tsa ho instola, sheba Buka ea Quickstart kapa Buka ea Litaelo bakeng sa mojule o itseng. Likhokahano tsa cable le mokhoa oa ho hokela ho caddy li fapana ho ea ka mojule.
4.3 Tekanyetso ea mojule
Ha o qala ho kenya mojule ho Flex, o hloka ho tsamaisa tlhophiso ea boemo bo ikemetseng. Ts'ebetso ena e ts'oana le ho lekanya maemo bakeng sa lisebelisoa, 'me e netefatsa hore Flex e fallela libakeng tse nepahetseng hantle bakeng sa ts'ebetso e nepahetseng ea protocol. Nakong ea ho lekanya, Flex e tla fallela libakeng tse nang le adaptara ea module calibration, e shebahalang e tšoana le li-square tsa calibration tseo e leng karolo ea li-slots tse khonang ho tlosoa.

Li-adapter tsa calibration tsa Heater-Shaker, Mocheso, le Thermocycler Modules.
Tekanyo ea mojule ea hlokahala bakeng sa limmojule tsohle tse kentsoeng ka caddy: Heater-Shaker, Temperature, le Thermocycler Modules. Magnetic Block ha e hloke ho lekanyetsoa, 'me e se e loketse ho sebelisoa hang ha u e beha mokatong oa sekepe.

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Nako ea ho lekanya li-module
Flex e u khothaletsa ka bo eona ho etsa calibration ha o hokela le ho kenya motlakase mojuleng o se nang data ea calibration e bolokiloeng. (O ka hlakola tlhahiso ena, empa u ke ke ua khona ho tsamaisa liprothokholo ka module ho fihlela u e lekanya.)
Ha u se u qetile ho lekanya, Flex e boloka data ea calibration le nomoro ea seriale ea module bakeng sa tšebeliso ea nako e tlang. Flex e ke ke ea u khothaletsa ho nchafatsa ntle le haeba u hlakola data ea calibration ea mojule oo litlhophisong tsa liroboto. O ka kenya le ho tima module oa hau ka bolokolohi, kapa oa e isa sebakeng se seng sa mokatong o mong, ntle le ho hloka ho nchafatsa. Haeba u batla ho nchafatsa, u ka qala ts'ebetso ka nako efe kapa efe ho tsoa kareteng ea module ho Opentrons App. (Recalibration ha e fumanehe ho tsoa ho "touchscreen".
Mokhoa oa ho lekanya li-module
Litaelo tse skrineng sa ho ama kapa ho Opentrons App li tla u tataisa mabapi le mokhoa oa ho lekanya. Ka kakaretso, mehato ke:
1. Bokella lisebelisoa tse hlokahalang, ho kenyeletsoa adaptara ea calibration ea module le probe ea calibration ea pipette. 2. Beha adaptara ea calibration holim'a mojulu 'me u netefatse hore e boemong bo felletseng.
Li-module tse ling li ka hloka hore u fase adaptara mojuleng. 3. Hokela probe ea calibration ho pipette. 4. Flex e tla itsamaela e ama lintlha tse itseng ho adaptara ea calibration ebe e boloka tsena
litekanyetso tsa calibration bakeng sa tšebeliso ea nako e tlang.
Hang ha litekanyo li felile 'me u tlositse adaptara le probe, mojule o tla be o loketse ho sebelisoa liprothokholeng.
Ka nako efe kapa efe, u ka khona view 'me u laole lintlha tsa tlhahlobo ea mojule oa hau ho Opentrons App. Eya ho Litlhophiso tsa Robot bakeng sa Flex ea hau ebe u tobetsa konopo ea Calibration.

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4.4 Heater-Shaker Mojule oa GEN1

Likarolo tsa Heater-Shaker
CHESA LE TŠIKINELA
The Heater-Shaker e fana ka ho futhumatsa ka mokatong le ho sisinyeha ha orbital. Mojule o ka futhumatsoa ho 95 ° C, ka mocheso o latelang oa profile:
Sebaka sa mocheso: 37 °C Ho nepahala ha mocheso: ±95 °C ho 0.5 °C Ho lumellana ha mocheso: ±55 °C ho 0.5 °CRamp sekhahla: 10 °C/min
Mojule o ka sisinya sampho tloha ho 200 ho isa ho 3000 rpm, ka pro e latelang e sisinyehangfile:
Bophara ba Orbital: 2.0 mm Tsela ea Orbital: Lebelo la lebelo la Clockwise: 200 rpm Ho nepahala ha lebelo: ± 3000 rpm
Mojule o na le latch ea labware e matla bakeng sa ho boloka lipoleiti ho mojulung pele o sisinyeha.

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KHAOLO EA 4: LIMOLAO
LI-ADAPTER TSA THERMAL Ho hlokahala adaptara e tsamaellanang le mocheso bakeng sa ho eketsa labware ho Heater-Shaker. Li-adapter li ka rekoa ka kotloloho ho Opentrons ho https://shop.opentrons.com. Li-adapter tsa Thermal tse fumanehang hajoale li kenyelletsa:

Adapter ea Universal Flat

Adapter ea PCR

Adapter e Tebileng Hantle

Adapter e ka tlase ea 96

PUSELETSO SOFETA
The Heater-Shaker e hlophisehile ka botlalo ho Protocol Designer le Python Protocol API. Python API e boetse e lumella hore mehato e meng ea protocol e etsoe ka mokhoa o ts'oanang ha Heater-Shaker e ntse e sebetsa. Sheba litaelo tse sa thibeleng litokomaneng tsa API bakeng sa lintlha tse mabapi le ho eketsa mehato e bapileng ho liprothokholo tsa hau.
Ka ntle ho liprothokholo, Opentrons App e ka bonts'a boemo ba hona joale ba Heater-Shaker 'me e ka laola ka ho toba heater, shaker le latch labware.

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KHAOLO EA 4: LIMOLAO

Litlhaloso tsa Heater-Shaker

Dimensions Weight Module ho kenya motlakase Kenyeletso ya adaptara ya motlakase Mains phepelo voltage fluctuation Overvoltage Tšebeliso ea matla

152 × 90 × 82 mm (L/W/H) 1.34 kg 36 VDC, 6.1 A 100 VAC, 240/50 Hz ±60% Sehlopha II Ho se sebetse: 10 W

Ka tloaelo: Ho sisinyeha: 4 W Ho futhumatsa: 11 W Ho futhumatsa le ho tsukutleha: 10 W

Maemo a tikoloho Thempereichara ea tikoloho Mongobo o lekantsoeng Altitude tšilafalo tekanyo

Boholo: 125 W Tšebeliso ea ka tlung feela 130 °C Ho fihla ho 20%, e sa koaheleng Ho fihlela ho 25 m ka holim'a bophahamo ba leoatle 80

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KHAOLO EA 4: LIMOLAO
4.5 Thibelo ea Magnetic GEN1

Likarolo tsa Magnetic Block
Opentrons Magnetic Block GEN1 ke sets'oants'o sa poleiti ea seliba sa 96 sa makenete. Li-block tsa Magnetic li sebelisoa ho liprothokholo tse itšetlehileng ka magnetism ho hula likaroloana ho tsoa ho fanyeha le ho li boloka ka har'a lipoleiti tsa seliba nakong ea ho hlatsoa, ho hlatsoa kapa ho etsa lits'ebetso tse ling. Bakeng sa mohlalaample, boitokisetso ba NGS bo ikemetseng; ho hloekisa DNA, RNA, kapa liprotheine tsa genomic le mitochondrial; le mekhoa e meng ea ho ntša ke linyeoe tsa tšebeliso tse ka kenyelletsang li-blocks tsa makenete.
LIEKETSENG MAKANETE
Magnetic Block ha e na matla, ha e na lisebelisoa tsa elektroniki, 'me ha e tsamaise lifaha tsa makenete holimo kapa tlase ho tharollo. Liliba li na le li-magnets tse 96 tsa matla a phahameng a neodymium tse kentsoeng betheng e tletseng selemo, e thusang ho boloka mamello pakeng tsa boloko le li-pipette ha ho ntse ho etsoa liprothokholo tse ikemetseng.
PUSELETSO SOFETA
Magnetic Block GEN1 e hlophisehile ka botlalo ho Protocol Designer le Python Protocol API.

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KHAOLO EA 4: LIMOLAO

Ka ntle ho liprothokholo, leha ho le joalo, sekirini sa touch le Opentrons App ha li tsebe ebile ha li khone ho bonts'a boemo ba hajoale ba Magnetic Block GEN1. Ena ke mojule o se nang matla. Ha e na lisebelisoa tsa elektroniki kapa tsa mochini tse ka buisanang le roboto ea Flex. U "laola" Thibelo ea Magnetic ka liprothokholo tse sebelisang Opentrons Flex Gripper ho eketsa le ho tlosa lisebelisoa tsa lisebelisoa mojulung ona.

Litlhaloso tsa Magnetic Block

Ditekanyo Boima ba Mojule Matla a Magnet Kereiti ya Maemo a Tikoloho Mocheso wa tikoloho Mongobo o lekanyeditsweng Altitude Pollution degree

136 × 94 × 45 limilimithara (L/W/H) 1.13 lik'hilograma Ha ho na, mojule ha e na matla a N52 neodymium Ka hare ho sebelisoa feela 20 °C 25%, e sa tsitsang Ho fihlela ho 30 m ka holim'a bophahamo ba leoatle 80

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KHAOLO EA 4: LIMOLAO
4.6 Mojule oa Mocheso GEN2

Likarolo tsa Module oa Mocheso
CHESA LE KHANYA
Opentrons Temperature Module GEN2 ke mojule oa poleiti e chesang le e batang. Hangata e sebelisoa ho liprothokholo tse hlokang ho futhumatsa, ho pholisa, kapa ho fetoha ha mocheso. Mojule o ka fihla le ho boloka mocheso ho tloha ho 4 °C ho isa ho 95 °C ka metsotso e seng mekae, ho ipapisitse le tlhophiso le likahare tsa module.
LIBOLO MOCHESO
Ho tšoara labware ka mocheso, mojule o sebelisa li-block tsa aluminium tse futhumatsang. Mojule ona o tla le li-block tsa 24well le 96-well tse futhumatsang hantle. The Temperature Module caddy e tla le boloko bo tebileng ba seliba le boloko bo tlase bo sephara bo etselitsoeng ho sebelisoa le Flex Gripper. Li-blocks li na le li-tubes tsa 1.5 mL le 2.0 mL, lipoleiti tsa PCR tse 96 hantle, likhoele tsa PCR, lipoleiti tse tebileng tsa seliba, le lipoleiti tse tlase tse bataletseng.

OPENTRONS FLEX

KHAOLO EA 4: LIMOLAO
Tlhokomeliso: Mojule o boetse o tsamaea ka sekepe se nang le sekoti se ka tlase bakeng sa OT-2. Se ke oa sebelisa block ea OT-2 le Flex. Karolo e ka tlase e bataletseng ea Flex e na le mantsoe "Opentrons Flex" holim'a eona. E 'ngoe ea OT-2 ha e joalo.

24 - seliba sa mocheso oa mocheso

96 - seliba sa mocheso oa mocheso

Sebaka se tebileng sa mocheso oa mocheso

Sebaka se tlase sa mocheso oa Flex

LIBATE TSA METSI LE CHESA
Hobane moea ke insulator e ntle ea mocheso, likheo pakeng tsa labware le "thermal block" li ka ama ts'ebetso ea nako ea mocheso oa Mocheso oa Mocheso. Ho kenya metsi a seng makae ka har'a li-24- kapa 96-lithako tsa mocheso ho felisa likheo tsa moea le ho ntlafatsa bokhoni ba ho futhumatsa. Palo e nepahetseng ea metsi e ipapisitse le boloko ba mocheso le labware. Sheba Temperature Module White Paper bakeng sa likhothaletso tse ling.

PUSELETSO SOFETA
The Temperature Module e hlophisehile ka botlalo ho Protocol Designer le Python Protocol API.
Ka ntle ho liprothokholo, Opentrons App e ka bonts'a boemo ba hajoale ba Temperature Module mme e ka laola ka kotloloho mocheso oa poleiti ea holim'a metsi.

OPENTRONS FLEX

KHAOLO EA 4: LIMOLAO

Litlhaloso tsa Module oa Mocheso

Litekanyo Weight Module matla
Maemo a tikoloho Thempereichara ea tikoloho Mongobo o lekantsoeng Altitude tšilafalo tekanyo

194 × 90 × 84 mm (L/W/H) 1.5 kg Kenyeletso: 100 VAC, 240/50 Hz, 60 A Output: 4.0 VDC, 36 A, 6.1 W max Tshebediso ya ka hare feela <219.6 °C (e kgothaletswa hore o pholile ka ho feletseng ) Ho fihla ho 22%, e sa koaheleng Ho fihlela ho 60 m ka holim'a bophahamo ba leoatle 2000

4.7 Thermocycler Module GEN2

OPENTRONS FLEX

KHAOLO EA 4: LIMOLAO
Likarolo tsa Thermocycler
Opentrons Thermocycler Module GEN2 ke thermocycler e itirisang ka botlalo, e fana ka PCR e se nang matsoho ka sebopeho sa poleiti ea liliba tse 96. Sekoaelo sa eona se futhumetseng le tiiso e lahloang li lekana ka thata holim'a poleiti, ho netefatsa hore sample ho futhumatsa le moya o fokolang.
HO FUTA LE HO PHOLISA Thermocycler block e ka futhumatsa le ho pholile, 'me sekoahelo sa eona se ka futhumatsa, ka mocheso o latelang oa pro.file: Mocheso oa mocheso oa "Thermal block": 4 °C Thermal block boholo ba ho futhumatsa ramp sekhahla: 4.25 °C/s ho tloha GEN2 ambient ho 95 °C Thermal block maximum cooling ramp sekhahla: 2.0 °C/s ho tloha 95 °C ho ea ho ambient Mocheso oa mocheso oa Sekoahelo: 37 °C Ho nepahala ha mocheso oa sekoahelo: ± 110 °C Sekoahelo se ikemetseng se ka buloa kapa sa koaloa ha ho hlokahala nakong ea ts'ebetso ea protocol.
THERMOCYCLER PROFILES Thermocycler e ka etsa profiles: ho tsamaea ka libaesekele ka tatellano ea lithempereichara tsa block ho etsa liketso tse sa utloeng mocheso.
RUBBER AUTOMATION SEALS Thermocycler e tla le litiiso tsa ho iketsetsa rabara ho thusa ho fokotsa mouoane. Setiiso se seng le se seng se tlameha ho hloekisoa pele se sebelisoa 'me se ka sebelisoa bakeng sa ho matha ka makhetlo a mangata. Litiiso tse ling li ka rekoa ka kotloloho ho Opentrons ho https://shop.opentrons.com.
TSAMAISO EA SOFTWARE Thermocycler e hlophisehile ka botlalo ho Protocol Designer le Python Protocol API. Ka ntle ho liprothokholo, Opentrons App e ka bonts'a boemo ba hajoale ba Thermocycler mme e ka laola ka kotloloho mocheso oa block, mocheso oa sekwahelo le boemo ba sekwahelo.

OPENTRONS FLEX

KHAOLO EA 4: LIMOLAO

Litlhaloso tsa Thermocycler

Litekanyo (sekoahelo se butsoe) Litekanyo (sekoahelo se koetsoe) Boima (ho kenyeletsoa le kotopo e ka morao) Adapter ea matlatage Adapter ea hona joale ea Overvoltage Maemo a tikoloho Thempereichara ea tikoloho Mongobo o lekanyelitsoeng oa Altitude Litlhoko tsa moea

244.95 × 172 × 310.1 mm (L/W/H) 244.95 × 172 × 170.35 limilimithara (L/W/H) 8.4 kg 100 V ho 240/50 Hz 60 A Category II Tšebeliso ea ka hare 8.5 °C5 feela 20 °C (e amohelehang) 25%, e sa fokotseheng Ho fihlela ho 2 m ka holim'a bophahamo ba leoatle Bonyane 40 cm / 30 pakeng tsa yuniti le lebota

OPENTRONS FLEX

KHAOLO EA 5
Lesebelisoa
Khaolo ena e akaretsa lintho tse Laeboraring ea Opentrons Labware eo u ka e sebelisang le Opentrons Flex le Opentrons Flex Gripper. E boetse e akaretsa li-labware tsa tloaelo, 'me bakeng sa basebelisi ba rona ba matla, e hokahanya likarolo tsa labware ho JSON ea bona e tsamaellanang. file litlhaloso.
O ka reka labware ho bahlahisi ba lisebelisoa tsa mantlha kapa lebenkeleng la Opentrons ho https://shop.opentrons.com. Hape, Opentrons e lula e sebetsa ho netefatsa litlhaloso tse ncha tsa labware. Sheba Laeborari ea Labware (e hokahaneng ka holimo) bakeng sa manane a morao-rao.
5.1 Likhopolo tsa Labware
Labware e akaretsa ho feta feela lintho tse behiloeng mokatong le tse sebelisoang ho protocol. Ho o thusa ho utloisisa labware ea Opentrons, ha re hlahlobe sehlooho sena ho tsoa liponong tse tharo tse fapaneng. Bakeng sa Opentrons Flex, labware e kenyelletsa lintho tse Laeboraring ea rona ea Labware, data e hlalosang karolo e 'ngoe le e 'ngoe ea lisebelisoa, le lisebelisoa tsa tloaelo.
Lab e le lisebelisoa
Laeborari ea Labware e kenyelletsa ntho e 'ngoe le e' ngoe eo u ka e sebelisang ka boiketsetso ka Opentrons Flex. Tsena ke lisebelisoa tse tšoarellang le lintho tse ka jeoang tseo u sebetsang le tsona, u li sebelisa hape, kapa u li lahla ha u ntse u sebelisa protocol. Ha ho hlokahale hore u nke mehato efe kapa efe e khethehileng ho sebetsa ka lintho tse Laeboraring ea Labware. Roboto ea Flex e tseba ho sebetsa le ntho e 'ngoe le e' ngoe e laebraring ka bo eona.
Lab e le data
Boitsebiso ka Labware bo bolokoa ho Javascript object notation (JSON) files le .json file katoloso. E JSON file e kenyelletsa litekanyo tsa sebaka (bolelele, bophara, bophahamo), bophahamo ba molumo (L, mL), le metrics e meng e hlalosang likarolo tsa bokaholimo, libopeho tsa tsona le libaka. Ha o sebelisa protocol, Flex e bala tsena .json files ho tseba hore na labware ke eng setulong le mokhoa oa ho sebetsa ka eona.

OPENTRONS FLEX

KHAOLO EA 5: LABWARE
Labware e tloaelehileng
Labware e tloahelehileng ke lisebelisoa tse sa kenyellelitsoeng Laeboraring ea Labware kapa ke lisebelisoa tse entsoeng ke 'Mopi oa Custom Labware. Leha ho le joalo, ka linako tse ling mohopolo oa lisebelisoa tsa tloaelo o tla o imetsoe ke maikutlo a ho rarahana, litšenyehelo, kapa bothata. Empa, li-labware tsa moetlo ha lia lokela ho ba thata ho li utloisisa kapa ho li etsa. Ha re nke nakoana ho manolla mohopolo oa "custom labware".
Joalo ka mohlalaample, Laeborari ea Opentrons Labware e kenyelletsa lipoleiti tsa liliba tse 96 (200 L) tse tsoang Corning le BioRad, empa bahlahisi ba bang le bona ba etsa lipoleiti tsena tsa seliba. 'Me, ka lebaka la maemo a tloaelehileng a indasteri, liphapang pakeng tsa lintho tsena tsa lab tse fumanehang hohle li nyane. Leha ho le joalo, poleiti e tloaelehileng ea 200 L, 96-well e tsoang ho Stellar Scientific, Oxford Lab, kapa Krackeler Scientific (kapa morekisi ofe kapa ofe oa taba eo) ke "custom labware" bakeng sa Flex hobane ha e hlalosoe esale pele Laeboraring ea rona ea Labware. . Ho feta moo, liphapang tse nyane lipakeng tsa li-labware li ka ba le tšusumetso e kholo katlehong ea protocol ea hau. Ka lebaka lena, ho bohlokoa ho ba le tlhaloso e nepahetseng ea labware bakeng sa sesebelisoa ka seng seo u batlang ho se sebelisa ho protocol ea hau.
Hape, le hoja labware e tloaelehileng e ka ba sesebelisoa sa esoteric, sa nako e le 'ngoe, boholo ba nako e mpa e le malebela, lipoleiti, liphaephe le liraka tse sebelisoang letsatsi le leng le le leng lilaboratoring lefatšeng ka bophara. Hape, phapang e le 'ngoe feela lipakeng tsa Opentrons labware le labware ea tloaelo ke ntho e ncha ha e hlalosoe esale pele ho software e fang roboto matla. Flex e khona, 'me ea sebetsa, e sebetsa le lintho tse ling tsa mantlha tsa labware kapa ntho e ikhethang, empa o hloka ho hatisa litšobotsi tsa ntho eo ka tlhaloso ea labware JSON. file le ho kenya data eo ho Opentrons App. Sheba karolo ea Custom Labware Definitions ka tlase bakeng sa lintlha tse ling.
Ho akaretsa, labware e kenyelletsa:
Ntho e ngoe le e ngoe e Laeboraring ea Opentrons Labware. Litlhaloso tsa Lab: data ho JSON file e hlalosang libopeho, boholo, le bokhoni ba lintho ka bomong
joalo ka lipoleiti tsa seliba, malebela, matamo, joalo-joalo. Labware e tloahelehileng, e leng lintho tse sa kenyelletsoeng Laeboraring ea Labware.
Ka mor'a reviewHa re nahana ka mehopolo ena ea bohlokoa, a re hlahlobeng lihlopha le lintho tse Laeboraring ea Opentrons Labware. Ka mor'a moo, re tla qetella khaolo ka ho qetelaview ea likarolo tsa data tsa labware file 'me u akaretse likarolo le lits'ebeletso tsa Opentrons tse u thusang hore u thehe lisebelisoa tse ikhethileng.

OPENTRONS FLEX

KHAOLO EA 5: LABWARE

5.2 Matamo a metsi
Opentrons Flex e sebetsa ka mokhoa o ikhethileng ka matamo a le mong le a nang le liliba tse ngata tse thathamisitsoeng ka tlase. Ho sebelisa matamo ana ho thusa ho fokotsa moroalo oa mosebetsi oa hau oa pele hobane a itokiselitse ho tsoa ka ntle ho lebokose. Lintlha tsa polokelo li fumaneha hape Laeboraring ea Opentrons Labware.

Matamo a seliba se le seng

Litlhaloso tsa Moetsi

Agilent

290 mL V tlase

Aksijene

90 ml ka tlase e bataletseng

SEHLAHA

195 ml ka tlase e bataletseng

290 mL V tlase

Lebitso la phalliso ea API
agilient_1_ reservoir_290ml
axygen_1_ reserve_90ml
nest_1_ reservoir_195ml
nest_1_ reservoir_290ml

Libaka tse nang le liliba tse ngata

Litlhaloso tsa Moetsi

SEHLAHA

12 liliba 15 mL / seliba V tlase

USA Saense

12 liliba 22 mL / seliba V tlase

Lebitso la phalliso ea API nest_12_ reservoir_15ml
usascientific_12_ reservoir_22ml

OPENTRONS FLEX

KHAOLO EA 5: LABWARE
Matamo le litlhaloso tsa API
Laeborari ea Opentrons Labware e hlalosa litšobotsi tsa matamo a thathamisitsoeng ka holimo ho JSON e arohaneng files. Roboto le Opentrons Python API li itšetlehile ka litlhaloso tsena tsa JSON ho sebetsa le labware e sebelisoang ke liprothokholo tsa hau. Bakeng sa mohlalaample, ha o sebetsa le API, mosebetsi oa ProtocolContext.load_labware o amohela mabitso ana a labware e le liparamente tse sebetsang khoutu ea hau. Mabitso a khokahanyo a API a hokahana le litlhaloso tsa polokelo ea polokelo sebakeng sa polokelo ea Opentrons GitHub.
Custom reservoir labware
Leka ho iketsetsa tlhaloso ea labware ka Opentrons Labware Creator haeba polokelo eo u batlang ho e sebelisa e sa thathamisoa mona. Tlhaloso ea tloaelo e kopanya litekanyo tsohle, metadata, libopeho, bophahamo ba molumo, le lintlha tse ling ho JSON. file. Opentrons Flex e hloka tlhahisoleseling ena ho utloisisa mokhoa oa ho sebetsa ka lisebelisoa tsa hau tsa tloaelo. Sheba karolo ea Custom Labware Definitions bakeng sa lintlha tse ling.
5.3 Lipoleiti tsa seliba
Opentrons Flex e sebetsa ka ho sa feleng ka lipoleiti tsa seliba tse thathamisitsoeng ka tlase. Ho sebelisa lipoleiti tsena tsa seliba ho thusa ho fokotsa moroalo oa hau oa ho sebetsa esale pele hobane li itokiselitse ho tsoa ka har'a lebokose. Tlhahisoleseding ea poleiti ea hantle e fumaneha hape Laeboraring ea Opentrons Labware.

OPENTRONS FLEX

KHAOLO EA 5: LABWARE

Lipoleiti tse 6 tsa seliba
Moetsi oa Corning

Litlhaloso
6 liliba 16.8 mL / seliba seliba seliba, sephara tlaase

Lebitso la mojaro oa API corning_6_wellplate_16.8ml_flat

Lipoleiti tse 12 tsa seliba
Moetsi oa Corning

Litlhaloso
12 liliba 6.9 mL / seliba seliba seliba, sephara tlaase

Lebitso la mojaro oa API corning_12_wellplate_6.9ml_flat

Lipoleiti tse 24 tsa seliba
Moetsi oa Corning

Litlhaloso
24 liliba 3.4 mL / seliba seliba seliba, sephara tlaase

Lebitso la mojaro oa API corning_24_wellplate_3.4ml_flat

Lipoleiti tse 48 tsa seliba
Moetsi oa Corning

Litlhaloso
48 liliba 1.6 mL / seliba seliba seliba, sephara tlaase

Lebitso la mojaro oa API corning_48_wellplate_1.6ml_flat

OPENTRONS FLEX

KHAOLO EA 5: LABWARE

Lipoleiti tse 96 tsa seliba
Moetsi oa Bio-Rad Corning NEST
Opentrons Thermo Scientific
USA Saense

Litlhaloso
96 liliba 200 µL / seliba seliba selikalikoe, V tlase
Liliba tse 96 360 µL/ seliba Liliba tse chitja, tse tlase tse bataletseng
96 liliba 100 µL / seliba Circle liliba, V tlase PCR full skirt
Liliba tse 96 200 µL/ seliba Liliba tse chitja, tse tlase tse bataletseng
Liliba tse tebileng tse 96 2000 µL / liliba tsa Sekwere, V tlase
Liliba tse thata tse 96 200 µL / liliba tse selikalikoe, V tlase PCR moleka o felletseng
Liliba tse tebileng tsa Nunc 96 1300 µL / seliba se chitja, U tlase
Liliba tse tebileng tsa Nunc 96 2000 µL / seliba se chitja, U tlase
96 liliba tse tebileng 2.4 mL / seliba Sekwere liliba, U tlase

Lebitso la mojaro la API biorad_96_wellplate_200ul_pcr
corning_96_wellplate_360ul_flat
nest_96_wellplate_100ul_pcr_full_skirt
nest_96_wellplate_200ul_flat
nest_96_wellplate_2ml_deep
opentrons_96_wellplate_200ul_pcr_full_ skirt
thermoscientificnunc_96_wellplate_ 1300ul thermoscientificnunc_96_wellplate_ 2000ul usascientific_96_wellplate_2.4ml_deep

OPENTRONS FLEX

KHAOLO EA 5: LABWARE

Lipoleiti tse 384 tsa seliba

Bio-Rad e sebelisitsoeng ke moetsi
Corning

Litlhaloso
384 liliba 40 µL / seliba seliba selikalikoe, V tlase
384 liliba 50 µL / seliba seliba selikalikoe, V tlase
384 liliba 112 µL / seliba Square liliba, tlaase bataletseng

Lebitso la mojaro la API appliedbiosystemsmicroamp_384_ wellplate_40ul biorad_384_wellplate_50ul
corning_384_wellplate_112ul_flat

Li-adapter tsa poleiti hantle
Lipoleiti tsa aluminium tse thathamisitsoeng ka tlase ke li-adapter tsa mocheso bakeng sa mojule oa Opentrons Heater-Shaker GEN1. U ka sebelisa litlhaloso tsena tsa adaptara e ikemetseng ho kenya Opentrons e netefalitsoeng kapa lisebelisoa tsa tloaelo ka holim'a Heater-Shaker.

Adapter mofuta Opentrons Universal Flat Heater-Shaker Adapter Opentrons 96 PCR Heater-Shaker Adapter Opentrons 96 Deep Well Heater-Shaker Adapter Opentrons 96 Flat Bottom Heater-Shaker Adapter

API lebitso la mojaro opentrons_universal_flat_adapter opentrons_96_pcr_adapter opentrons_96_deep_well_adapter opentrons_96_flat_bottom_adapter

U ka kenya hape adaptara le labware ka tlhaloso e le 'ngoe. Laeborari ea rona ea Labware e kenyelletsa li-adapter tse 'maloa tse lokiselitsoeng esale pele le li-labware tse etsang hore Heater-Shaker e itokisetse ho sebelisoa ka ntle ho lebokose.

OPENTRONS FLEX

KHAOLO EA 5: LABWARE

Tlhokomeliso: Se ke oa sebelisa tlhaloso e kopantsoeng haeba u hloka ho tsamaisa labware holim'a kapa ho tsoa ho Heater-Shaker nakong ea protocol ea hau, ebang ke ka gripper kapa ka letsoho. Sebelisa tlhaloso ea adaptara e ikemetseng ho e-na le hoo.

Motsoako oa li-adapter/labware

Lebitso la phalliso ea API

Opentrons 96 Deep Well Heater-Shaker Adapter e nang le NEST Deep Well Plate 2 mL

opentrons_96_deep_well_adapter_nest_ wellplate_2ml_deep

Adapter ea Opentrons 96 Flat Bottom Heater-Shaker e nang le NEST 96 Well Plate 200 µL Flat

opentrons_96_flat_bottom_adapter_nest_ wellplate_200ul_flat

Adapter ea Opentrons 96 PCR Heater-Shaker e nang le NEST Well Plate 100 µL

opentrons_96_pcr_adapter_nest_wellplate_ 100ul_pcr_full_skirt

Adapter ea Opentrons Universal Flat Heater-Shaker e nang le Corning 384 Well Plate 112 µL Flat

opentrons_universal_flat_adapter_corning_384_ wellplate_112ul_flat

Li-adapter li ka rekoa ka kotloloho ho Opentrons ho https://shop.opentrons.com.

Lipoleiti tsa hantle le litlhaloso tsa API
Laeborari ea Opentrons Labware e hlalosa litšobotsi tsa lipoleiti tsa liliba tse thathamisitsoeng ka holimo ho JSON e arohaneng files. Robot ea Flex le Opentrons Python API li itšetlehile ka litlhaloso tsena tsa JSON ho sebetsa le labware e sebelisoang ke liprothokholo tsa hau. Bakeng sa mohlalaample, ha o sebetsa le API, mosebetsi oa ProtocolContext.load_labware o amohela mabitso ana a labware e le liparamente tse sebetsang khoutu ea hau. Mabitso a hokahaneng a API a hokahana le litlhaloso tsa li-labware tsa seliba sebakeng sa polokelo ea Opentrons GitHub.

Custom hantle poleiti labware
Leka ho sebelisa Opentrons Labware Creator ho etsa tlhaloso ea "labware" e tloaelehileng haeba poleiti ea seliba eo u ka ratang ho e sebelisa e sa thathamisoa mona. Tlhaloso ea tloaelo e kopanya litekanyo tsohle, metadata, libopeho, bophahamo ba molumo, le lintlha tse ling ho JSON. file. Opentrons Flex e bala tlhahisoleseling ena ho utloisisa mokhoa oa ho sebetsa le lisebelisoa tsa hau tsa tloaelo. Sheba karolo ea Custom Labware Definitions bakeng sa lintlha tse ling.

OPENTRONS FLEX

KHAOLO EA 5: LABWARE

5.4 Malebela le liraka tsa malebela
Malebela a Opentrons Flex a tla ka boholo ba 50 µL, 200 µL, le 1000 μL. Tsena ke malebela a hlakileng, a sa sebetseng a polypropylene a fumanehang ka li-filters kapa ntle le tsona. Li phuthetsoe ka liraka tse nang le malebela a 96 'me ha li na DNase, RNase, protease, pyrogens, DNA ea motho, endotoxins, le PCR inhibitors. Li-racks li boetse li kenyelletsa linomoro tsa lotho le matsatsi a ho felloa ke nako.
Malebela a lipeipi tsa Flex a sebetsa le lipeipi tsa Opentrons Flex 50 µL le 1000 µL lipeakanyong tsa 1-, 8-, le 96-channel. Ha o ntse o ka beha malebela afe kapa afe a Flex ho lipeipi tsa 50 L le 1000 L, leka ho bapisa ntlha eo le matla a lekantsoeng a pipette. Bakeng sa mohlalaampLeha ho le joalo, e kanna ea ba ntho e makatsang ho beha ntlha ea 1000 L ho pipette ea 50 L. Bakeng sa pipette ea 1000 L, ka sebele u ka sebelisa ntlha ea 50 L, 200 L, kapa 1000 L.

Liraka tsa malebela
Malebela a sa sefeng le a tlhotliloeng a bokelloa ka har'a rake e nang le poleiti e ka sebelisoang hape, poleiti e bohareng e ts'oereng malebela a 96, le sekwahelo.

Keletso rack ka bophahamo ba modumo 50 µL 200 µL 1000 µL

Lebitso la phalliso ea API
E sa sefisoang: opentrons_flex_96_tiprack_50ul E hloekisitsoeng: opentrons_flex_96_filtertiprack_50ul
E sa sefisoang: opentrons_flex_96_tiprack_200ul E hloekisitsoeng: opentrons_flex_96_filtertiprack_200ul
E sa sefisoang: opentrons_flex_96_tiprack_1000ul E hloekisitsoeng: opentrons_flex_96_filtertiprack_1000ul

Ho thusa ka boitsebahatso, "tip rack mid-plates" e ngotsoe ka mebala ho latela boholo ba ntlha:
50 µL: magenta 200 L: mosehla 1000 µL: putsoa

OPENTRONS FLEX

KHAOLO EA 5: LABWARE

Ha o odara kapa o hlophisa bocha, malebela le liraka li tla ka litlhophiso tse peli tse fapaneng tse pakiloeng:
Racks: E na le li-racks tse phuthetsoeng ka thoko (plate ea motheo, poleiti e bohareng e nang le malebela, le sekwahelo). Litlhophiso tse hloekisitsoeng li molemo ka ho fetisisa ha bohloeki e le ba bohlokoa, ho qoba tšoaetso, kapa ha liprothokholi tsa hau li sa u lumelle ho sebelisoa hape.
Refills: E na le rack e le 'ngoe e felletseng (plate ea motheo, poleiti e bohareng e nang le malebela, le sekwahelo) le lijana tsa malebela. Litlhophiso tsa ho tlatsa li molemo ka ho fetisisa ha liprothokholo tsa hau li u lumella ho sebelisoa hape.
Tšebelisano ea Tippipette
Malebela a pipette a Flex a etselitsoe lipeipi tsa Opentrons Flex. Malebela a Flex ha a tsamaellane ka morao le lipeipi tsa Opentrons OT-2, hape u ka se sebelise malebela a OT-2 ho li-pipette tsa Flex.
Malebela a mang a tloaelehileng a indasteri a ka sebetsa le Flex pipettes, empa sena ha se khothalletsoe. Ho netefatsa ts'ebetso e nepahetseng, o lokela ho sebelisa malebela a Opentrons Flex feela ka lipeipi tsa Flex.
Adapter ea rack ea malebela
Pipette ea 96-channel e hloka adaptara ho hokela rack e felletseng ea malebela hantle. Nakong ea ts'ebetso ea ho kopanya, pipette e tsamaea holim'a adaptara, e theohela holim'a lithakhisa tse ntseng li kenngoa, ebe e hula malebela holim'a li-pipette ka ho phahamisa adaptara le rack ea ntlha.

OPENTRONS FLEX

KHAOLO EA 5: LABWARE

Tlhokomeliso: Sebelisa adaptara ea rack feela ha u nka malebela a mangata hang-hang. Beha liraka tsa malebela ka kotloloho mokatong oa sekepe ha u nka malebela a fokolang.
Tlhokomeliso: Kotsi ea ntlha. Beha matsoho hole le adaptara ea rack ha pipette e ntse e hokela malebela a pipette.

Adapter ea mofuta oa Opentrons Flex 96 Tip Rack Adapter

Lebitso la mojaro la API opentrons_flex_96_tiprack_adapter

Adapter ea rack e tsamaisana le Opentrons Flex Gripper. U ka sebelisa gripper ho beha li-racks tse ncha holim'a adaptara kapa ho nka le ho tsamaisa liraka tse sebelisitsoeng ka har'a chute ea litšila.

5.5 Li-tubes le li-tube racks

Sistimi ea Opentrons 4-in-1 Tube Rack e sebetsa le Opentrons Flex ka ho sa feleng. Ho sebelisa 4-in-1 tube rack ho thusa ho fokotsa moroalo oa hau oa pele oa mosebetsi hobane metsoako eo e fanang ka eona e itokiselitse ho iketsetsa ka ntle ho lebokose. Lintlha tse ling li fumaneha hape Laeboraring ea Opentrons Labware.

OPENTRONS FLEX

KHAOLO EA 5: LABWARE
Motsoako oa li-tube le rack
Opentrons 4-in-1 tube rack e ts'ehetsa mefuta e mengata ea boholo ba li-tube, e le 'ngoe kapa ka mefuta e fapaneng ea boholo (bolumo). Tsena li kenyelletsa: rack ea 6-tube bakeng sa li-tubes tse 50 mL (6 x 50 mL). 10-tube motsoako rack bakeng sa li-tubes tse 'nè tsa 50 mL le tse tšeletseng tsa 15 mL (4 x 50 mL, 6 x 15 mL). 15-tube rack bakeng sa 15 mL tubes (15 x 15 mL). 24-tube rack bakeng sa 0.5 mL, 1.5 mL, kapa 2 mL li-tubes (24 x 0.5 mL, 1.5 mL, 2 mL).
Tlhokomeliso: Li-tubes tsohle li cylindrical tse nang le bokatlase ba sebopeho sa V (conical) ntle le ha ho boletsoe ka tsela e 'ngoe.

6-tube liraka
Mofuta oa Tube 6 Phakoe 50 mL 6 NEST 50 mL
10-tube liraka
Mofuta oa Tube 4 Phakoe 50 mL 6 Phakoe 15 mL 4 NEST 50 mL 6 NEST 15 mL

API lebitso la mojaro opentrons_6_tuberack_falcon_50ml_conical opentrons_6_tuberack_nest_50ml_conical
API load name opentrons_10_tuberack_falcon_4x50ml_6x15ml_conical opentrons_10_tuberack_nest_4x50ml_6x15ml_conical

OPENTRONS FLEX

KHAOLO EA 5: LABWARE

15-tube liraka
Mofuta oa Tube 15 Phakoe 15 mL 15 NEST 15 mL

API lebitso la mojaro opentrons_15_tuberack_falcon_15ml_conical opentrons_15_tuberack_nest_15ml_conical

24-tube liraka

Mofuta oa Tube
24 Eppendorf Safe-Lock 1.5 mL 24 Eppendorf Safe-Lock 2 mL, U-shaped bottom 24 generic 2 mL screw cap 24 NEST 0.5 mL screw cap 24 NEST 1.5 mL screw cap 24 NEST 1.5 mL snap 24 m2 cap 24 m2 NEST XNUMX mL snap cap, tlase e nang le sebopeho sa U

API lebitso la mojaro opentrons_24_tuberack_eppendorf_1.5ml_safelock_snapcap opentrons_24_tuberack_eppendorf_2ml_safelock_snapcap
opentrons_24_tuberack_generic_2ml_screwcap opentrons_24_tuberack_nest_0.5ml_screwcap opentrons_24_tuberack_nest_1.5ml_screwcap opentrons_24_tuberack_nest_1.5ml_snapcap opentrons_24_tuberack_nest_2ml_screwcap opentrons_24_tuberack_nest_2ml_snapcap

Litlhaloso tsa Tube rack API
Laeborari ea Opentrons Labware e hlalosa litšobotsi tsa li-tube racks tse thathamisitsoeng ka holimo ho JSON e arohaneng files. Robot ea Flex le Opentrons Python API li itšetlehile ka litlhaloso tsena tsa JSON ho sebetsa le labware e sebelisoang ke liprothokholo tsa hau. Bakeng sa mohlalaample, ha o sebetsa le API, mosebetsi oa ProtocolContext.load_labware o amohela mabitso ana a labware e le liparamente tse sebetsang khoutu ea hau. Mabitso a hokahaneng a API a hokahana le litlhaloso tsa labware ea tube rack polokelong ea Opentrons GitHub.

OPENTRONS FLEX

KHAOLO EA 5: LABWARE
Custom tube rack labware
Leka ho iketsetsa tlhaloso ea "labware" ka "Opentrons Labware Creator" haeba motsoako oa li-tube le liraka tseo u ka ratang ho li sebelisa li sa thathamisoa mona. Tlhaloso ea tloaelo e kopanya litekanyo tsohle, metadata, libopeho, bophahamo ba molumo, le lintlha tse ling ho JSON. file. Opentrons Flex e bala tlhahisoleseling ena ho utloisisa mokhoa oa ho sebetsa le lisebelisoa tsa hau tsa tloaelo. Sheba karolo ea Custom Labware Definitions bakeng sa lintlha tse ling.
5.6 liboloko tsa aluminium
Aluminium e thibela sekepe ka Temperature Module GEN2 mme e ka rekoa ka thoko e le sete ea likarolo tse tharo. Setulo se kenyelletsa poleiti e tlase e bataletseng, boloko ba liliba tse 24, le boloko ba liliba tse 96.
Opentrons Flex e sebelisa li-block tsa aluminium ho tšoara sample li-tubes le lipoleiti tsa seliba ho Module oa Mocheso kapa ka kotloloho mokatong oa sekepe. Ha e sebelisoa le Temperature Module, li-block tsa aluminium li ka boloka sample li-tubes, li-strip tsa PCR, kapa lipoleiti mocheso o sa khaotseng oa pakeng tsa 4 °C le 95 °C.
Letlapa le tlase le bataletseng
Poleite e tlase e bataletseng bakeng sa likepe tsa Flex e nang le caddy ea Temperature Module 'me e tsamaisana le lipoleiti tse fapaneng tse tloaelehileng tsa ANSI/SLAS. Letlapa lena le bataletseng le fapane le poleiti e tsamaisoang ka Temperature Module ka boeona kapa sete e arohaneng ea likarolo tse tharo. E na le sebaka se pharaletseng sa ho sebetsa le likarolo tsa sekhutlo tse chamfered. Likarolo tsena li thusa ho ntlafatsa ts'ebetso ea Opentrons Flex Gripper ha o tsamaisa labware holim'a poleiti.
U ka tseba hore na u na le poleiti efe e ka tlase e bataletseng hobane ea Flex e na le mantsoe “Opentrons Flex” bokaholimong ba eona. E 'ngoe ea OT-2 ha e joalo.

OPENTRONS FLEX

KHAOLO EA 5: LABWARE

24-liliba tsa aluminium block
Sebaka sa seliba sa 24 se sebelisoa le motho ka mong sample linkho. Bakeng sa mohlalaample, e amohela sample linkho tse:
E be le bokatlase ba sebopeho sa V kapa sa U. Sireletsa litaba ka snap cap kapa sekoti se koetsoeng. Tšoara mokelikeli ka matla a 0.5 mL, 1.5 mL, le 2 mL.

96-liliba tsa aluminium block
Sebaka sa seliba sa 96 se tšehetsa mefuta e mengata e fapaneng ea lipoleiti tsa seliba. Bakeng sa mohlalaample, e amohela lipoleiti hantle tse leng:
Ho tsoa ho baetsi ba lipolanete tse kholo joalo ka Bio-Rad le NEST.
E entsoe ka botlaaseng ba sebopeho sa V, bokatlase ba sebopeho sa U, kapa bokatlase bo bataletseng.
E entsoe ka liliba tsa 100 µL kapa 200 μL.
E boetse e tsamaisana le likhoele tsa generic tsa PCR.

Li-adapter tse ikemetseng
Thermal block Flex e bataletseng ka tlase poleiti ea 24-liliba aluminium boloko ba 96-liliba aluminium boloko

API load name opentrons_aluminium_flat_bottom_plate Sheba metsoako ea labware ka tlase. opentrons_96_well_aluminium_block

Aluminium block labware metsoako
Laeborari ea Opentrons Labware e ts'ehetsa motsoako o latelang oa block, vial, le liplate tsa seliba, tseo le tsona li hlalosoang ka tlhaloso e arohaneng ea labware ea JSON. files. Roboto ea Flex le Opentrons Python API

OPENTRONS FLEX

KHAOLO EA 5: LABWARE
itšetleha ka litlhaloso tsena tsa JSON ho sebetsa le labware e sebelisoang ke liprothokholo tsa hau. Bakeng sa mohlalaample, ha o sebetsa le API, mosebetsi oa ProtocolContext.load_labware o amohela mabitso ana a labware e le liparamente tse sebetsang khoutu ea hau. Litafole tse ka tlase li thathamisa motsoako oa li-block/container le mabitso a amanang le API. Lihokelo li hokahana le litlhaloso tse tsamaellanang tsa JSON sebakeng sa polokelo sa Opentrons GitHub.
Tlhokomeliso: Li-tubes tsohle li na le botlaaseng ba sebopeho sa V ntle le ha ho boletsoe ka tsela e 'ngoe.

Motsoako oa lisebelisoa tsa lisebelisoa tsa aluminium tse 24 hantle

24-well block contents Generic 2 mL screw cap NEST 0.5 mL screw cap NEST 1.5 mL screw cap NEST 1.5 mL snap cap NEST 2 mL screw cap NEST 2 mL snap cap, tlase e bōpehileng joaloka U.

Lebitso la mojaro la API opentrons_24_aluminumblock_generic_2ml_screwcap opentrons_24_aluminiumblock_nest_0.5ml_screwcap opentrons_24_aluminumblock_nest_1.5ml_screwcap opentrons_24_aluminumblock_nest_1.5mlcaptropin_scrowp ns_24_aluminiumblock_nest_2ml_snapcap

Motsoako oa lisebelisoa tsa lisebelisoa tsa aluminium tse 96 hantle

Likahare tsa seliba tse 96 tsa Bio-Rad seliba sa 200 µL Generic PCR strip 200 µL NEST poleiti ea seliba 100 µL

API lebitso la mojaro opentrons_96_aluminumblock_biorad_wellplate_200uL opentrons_96_aluminumblock_generic_pcr_strip_200uL opentrons_96_aluminumblock_nest_wellplate_100uL

OPENTRONS FLEX

KHAOLO EA 5: LABWARE

5.7 Labware le Opentrons Flex Gripper
Le ha Opentrons Flex e sebetsa le lethathamo la lintho tsohle tse Laeboraring ea Labware, Opentrons Flex Gripper e tsamaisana le lintho tse itseng tsa labware feela. Hajoale, Gripper e ntlafalitsoe hore e sebelisoe le lintho tse latelang tsa labware.

Sehlopha sa Labware Lipoleiti tsa Deep Well Skirted ka ho Feletseng 96 Well Plates
Li-Racks tsa Tip (malebela a sa sefisoang le a tlhotliloeng)

Brands
Sehlaha sa 96 Seliba se Tebileng 2 mL
Opentrons Tough 96 Well Plate 200 µL PCR Full Skirt NEST 96 Well Plate 200 µL Flat
Opentrons Flex 96 Tip Rack 50 µL Opentrons Flex 96 Tip Rack 200 µL Opentrons Flex 96 Tip Rack 1000 µL

Tlhokomeliso: Bakeng sa liphetho tse ntle, sebelisa Flex Gripper feela ka labware e thathamisitsoeng ka holimo. Flex Gripper e ka sebetsa le lisebelisoa tse ling tse lumellanang le ANSI/SLAS, empa sena ha se khothalletsoe.

5.8 Litlhaloso tsa Custom labware
Joalo ka ha ho boletsoe qalong ea khaolo ena, labware ea tloaelo ke labware e sa thathamisoang Laeboraring ea Opentrons Labware. U ka sebelisa lintho tse ling tse tloaelehileng kapa tse ikhethang tsa labware ka Flex ka ho lekanya ka nepo le ho rekota litšobotsi tsa ntho eo le ho boloka data eo ho JSON. file. Ha e kenngoa ka har'a sesebelisoa, Flex le API li sebelisa data eo ea JSON ho sebelisana le lab ea hau. Opentrons e fana ka lisebelisoa le lits'ebeletso, tseo re tla li hlahloba ka tlase, ho u thusa ho sebelisa Flex ka lisebelisoa tsa tloaelo.

OPENTRONS FLEX

KHAOLO EA 5: LABWARE

Ho theha litlhaloso tsa lisebelisoa tsa lisebelisoa
Lisebelisoa le lits'ebeletso tsa Opentrons li thusa ho beha li-labware tse ikhethileng moo u ka li fihlelang. Likarolo tsena li kenyelletsa maemo a fapaneng a boiphihlelo le mekhoa ea ho sebetsa. Ho iketsetsa lab ea hau, le ho e sebelisa ka Opentrons Flex, ho thusa ho etsa hore roboto e be tlatsetso e matla le e sebetsang ho lab ea hau.

Custom LABWARE MMOPI
The Custom Labware Creator ke no-code, web-e thehiloeng sesebelisoa se sebelisang sebopeho sa graphical ho u thusa ho theha tlhaloso ea labware file. Moetsi oa Labware o hlahisa tlhaloso ea labware ea JSON file tseo o li kenyang ho Opentrons App. Kamora moo, labware ea hau ea tloaelo e fumaneha ho roboto ea Flex le Python API.

TŠEBELETSO EA LABWARE
Ikopanye le rona haeba labware eo u batlang ho e sebelisa e le sieo laebraring, haeba u sa khone ho iketsetsa litlhaloso, kapa hobane ntho e ikhethileng e kenyelletsa libopeho tse fapaneng, boholo, kapa litlolo tse ling tse hlalositsoeng ka tlase.

Labware eo u ka e hlalosang ho 'Mopi oa Labware
; Liliba le li-tubes li tšoana ebile lia tšoana. ; Mela eohle e arohane ka ho lekana
(sebaka se pakeng tsa mela sea lekana).
; Litšiea tsohle li arohane ka ho lekana (sebaka se pakeng tsa likholomo sea lekana).
; E lumellana hantle le sethala se le seng.

Labware Opentrons e hloka ho hlalosa; Liliba le libopeho tsa tube lia fapana. ; Mela ha e arohane ka ho lekana.
; Likholomo ha li arohane ka ho lekana.
; E nyane ho feta sekotjana sa deske e le 'ngoe (e hloka adaptara) kapa e hola li-slots tse ngata.

OPENTRONS FLEX

KHAOLO EA 5: LABWARE
Mona ke litšoantšo tse u thusang ho bona ka mahlo a kelelloamptse hlalositsoeng ka holimo. Kamehla Likholomo tsohle li arohane ka ho lekana 'me mela eohle e arohane ka ho lekana. Likholomo ha li hloke ho ba le sebaka se lekanang le mela.
Kamehla Grid ha ea tlameha ho ba bohareng ba labware.
Mela e Irregular e arohane ka ho lekana empa dikholomo ha di arohane ka ho lekana.
Litšiea/ mela e sa tloaelehang li arohane ka ho lekana empa liliba ha li tšoane.
Irregular Ho na le marang-rang a fetang bonngoe.
Sehlopha sa rona sa lisebelisoa tsa marang-rang se tla sebetsa ho utloisisa litlhoko tsa hau le ho u rala litlhaloso tse tloaelehileng tsa labware bakeng sa hau. Sheba lingoloa tsa tšehetso tse Kopang Tlhaloso ea Tloaelo ea Labware le foromo ea Kopo ea Tloaelo ea Labware bakeng sa tlhaiso-leseling e batsi. Ena ke tšebeletso ea chelete.

OPENTRONS FLEX

KHAOLO EA 5: LABWARE

PYTHON API
Le ha o sa khone ho etsa lisebelisoa tsa tloaelo ka API ea rona, o ka sebelisa lisebelisoa tsa tloaelo ka mekhoa e fumanehang ea API. Leha ho le joalo, o hloka ho hlalosa labware ea hau ea tloaelo pele ebe o e kenya ho Opentrons App. Ha u se u kentse lab ea hau ho Opentrons App, e fumaneha ho Python API le roboto. Sheba karolo ea Custom Labware Definitions ea litokomane tsa Python API bakeng sa boitsebiso bo eketsehileng. Ho fumana leseli mabapi le ho ngola mangolo a protocol ka API, bona karolo ea Python Protocol API khaolong ea ntlafatso ea Protocol.
JSON labware schema
E JSON file ke polane ea Opentrons e tloaelehileng le lisebelisoa tsa tloaelo. Sena file e na le ho hlophisa data ea labware ho latela litlhaloso tsa moralo tse behiloeng ke schema ea kamehla.
Leano ke foreime

Litokomane / Lisebelisoa

opentrons FLEX FLEX Opentrons Flex Open Source Liquid Handling Robot [pdf] Buka ea Taelo
FLEX Opentrons Flex Open Source Liquid Handling Robot, FLEX, Opentrons Flex Open Source Liquid Handling Robot, Flex Open Source Liquid Handling Robot, Robot e Bulehileng ea Liquid Handling Robot, Mohloli oa Mokelikeli oa Mokelikeli, Robot e sebetsanang le Mokelikeli, Robot ea ho tšoara, Robot

Litšupiso

Bukana ea Mosebelisi

FLEX Opentrons Flex Open Source Liquid Ho sebetsana le Robot

Opentrons Flex

Litlhaloso:

Litaelo tsa Tšebeliso ea Sehlahisoa:

1. Kemiso ea lisebelisoa le ho lekanya:

2. Phalliso:

3. Lihokelo:

4. Moqapi oa Protocol:

5. Python Protocol API:

6. OT-2 Protocols:

Lipotso Tse Botsoang Khafetsa (FAQ):

P: Nka rarolla bothata joang haeba roboto e sa tsamaee joalo ka
lebeletsoe?

P: A na nka sebelisa li-pipette tse tloaelehileng ka Opentrons Flex?

Litokomane / Lisebelisoa

Litšupiso

Tlohela maikutlo

Hlakola karabo

FLEX Opentrons Flex Open Source Liquid Ho sebetsana le Robot

Opentrons Flex

Litlhaloso:

Litaelo tsa Tšebeliso ea Sehlahisoa:

1. Kemiso ea lisebelisoa le ho lekanya:

2. Phalliso:

3. Lihokelo:

4. Moqapi oa Protocol:

5. Python Protocol API:

6. OT-2 Protocols:

Lipotso Tse Botsoang Khafetsa (FAQ):

P: Nka rarolla bothata joang haeba roboto e sa tsamaee joalo ka lebeletsoe?

P: A na nka sebelisa li-pipette tse tloaelehileng ka Opentrons Flex?

Litokomane / Lisebelisoa

Litšupiso

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Tlohela maikutlo

Hlakola karabo

P: Nka rarolla bothata joang haeba roboto e sa tsamaee joalo ka
lebeletsoe?