Nkọwa n'ozuzu: Lorem ipsum dolor nọdụ ala
amet, consectetur adipiscing elit. Sed na eiusmod tempor inciddunt
ut labore et dolore magna aliqua.
Nkọwapụta gburugburu: Lorem ipsum
dolor ọdụ amet, consectetur adipiscing elit. Dị ka eiusmod tempor
inciddunt ut labore et dolore magna aliqua.

Asambodo: Lorem ipsum dolor nọ ọdụ,
consectetur adipiscing elit. Sed do eiusmod tempor inciddunt ut
labore et dolore magna aliqua.
Nọmba usoro n'usoro: XXX-XXX-XXXX

Ntuziaka ojiji:

1. Nwụnye na nhazi akụrụngwa:

Soro usoro ndị edepụtara na ntuziaka maka pipette na gripper
echichi.

2. Mbugharị:

Maka mmegharị mkpụmkpụ, rụtụ aka na ngalaba 2.5 na ntuziaka. Maka
njem dị anya, soro ntuziaka enyere. Ntugharị izugbe
ndụmọdụ dịkwa.

3. Njikọ:

Gbaa mbọ hụ na njikọ ike kwesịrị ekwesị dị ka akọwapụtara n'akwụkwọ ntuziaka.
Jikọọ USB na ngwaọrụ inyeaka dị ka achọrọ. Njikọ netwọk
kwesịrị guzobe na-eso ntuziaka.

4. Onye Mmebe Protocol:

Ghọta ihe achọrọ maka Onye nrụpụta Protocol wee mụta otu esi eme ya
iji chepụta protocol ọhụrụ ma ọ bụ gbanwee ndị dị ugbu a dịka ụlọ nyocha gị
chọrọ.

5. Python Protocol API:

Jiri Python Protocol API nyochaa edemede na ịgba ọsọ.
Chọpụta atụmatụ Python-iche maka arụrụ arụ ọrụ.

6. OT-2 Usoro:

Mụta maka OT-2 Python protocols, OT-2 JSON protocols, na
Usoro modul magnetik maka ụdị nnwale dị iche iche.

Ajụjụ A na-ajụkarị (FAQ):

Ajụjụ: Kedu ka m ga-esi chọpụta nsogbu ma ọ bụrụ na robot anaghị emegharị dị ka
tụrụ anya?

A: Lelee njikọ ike, hụ na nhazi nke ọma
ngwa, ma chọpụta na enweghị ihe mgbochi na
ụzọ robot.

Ajụjụ: Enwere m ike iji pipettes omenala na Opentrons Flex?

A: Opentrons na-atụ aro iji pipettes dakọtara maka kacha mma
arụmọrụ na izi ezi.

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Opentrons Flex
Akwụkwọ ntuziaka
Ụlọ ọrụ Opentrons Labworks Inc.
Disemba 2023

Tebulu ọdịnaya
Okwu mmalite . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 Nhazi akwụkwọ ntuziaka a. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Ihe ndetu na ịdọ aka ná ntị . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Isi nke 1: Okwu Mmalite . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 1.1 Nabata na Opentrons Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Kedu ihe dị ọhụrụ na Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Flex ọdụ ọrụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 1.2 Ozi nchekwa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 akara nchekwa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Ịdọ aka ná ntị nchekwa eletrik . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Ịdọ aka ná ntị nchekwa agbakwunyere. . . . . . . . . . . . . . . . . . . . . . . . . . . .18 Nchekwa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 Nchekwa ndu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Uzuzu nsi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 mmiri mmiri na-ere ọkụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 1.3 Nrube isi n'usoro. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Nchekwa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 ndakọrịta elektrọnik. . . . . . . . . . . . . . . . . . . . . . . . .20 FCC ịdọ aka ná ntị na ndetu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Canada ISE nnabata . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Ịdọ aka ná ntị gburugburu ebe obibi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Asambodo Wi-Fi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Isi nke 2: Nwụnye na mbugharị . . . . . . . . . . . . . . . . . . . . . . . 22 2.1 chọrọ nchekwa na arụ ọrụ. . . . . . . . . . . . . . . . . . . . .22 Ebe a ga-edebe Opentrons Flex. . . . . . . . . . . . . . . . . . . . . . . .22 Ike oriri. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 Ọnọdụ gburugburu ebe obibi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 2.2 Unboxing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Mgbalị na oge achọrọ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Crate na nkwakọ ngwaahịa. . . . . . . . . . . . . . . . . . . . . . . . . . . .25 ngwaahịa ihe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 Nkebi nke 1: Wepu igbe ahụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27 Nkebi 2: Hapụ Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29 Nkebi 3: Mgbakọ ikpeazụ na ike na . . . . . . . . . . . . . . . . . . .32

2.3 Agba mbụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Ike na . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Jikọọ na netwọk ma ọ bụ kọmputa . . . . . . . . . . . . . . . . . . . . .36 Wụnye mmelite ngwanrọ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37 Tinye pendant kwụsị ihe mberede. . . . . . . . . . . . . . . . . . . . . . 38 Nye robot gị aha . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
2.4 Ntinye akụrụngwa na nhazi. . . . . . . . . . . . . . . . . 38 Ntinye pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 Gripper echichi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
2.5 Mbugharị . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 Ntugharị mkpụmkpụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Ngagharị anya . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Ndụmọdụ na-akpali akpali n'ozuzu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 ikpeazụ echiche banyere ịkwaga. . . . . . . . . . . . . . . . . . . . . . . . . . .44
Isi nke 3: Nkọwa Sistemu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 3.1 Akụkụ anụ ahụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45 Frame na mkpuchi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 Ogbe na ebe ọrụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 Stagmpaghara . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47 Ihe ndozi oche. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 Ebe mkpofu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49 Stagoghere mpaghara oghere. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49 Usoro mmegharị. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 ihuenyo mmetụ na ihe ngosi LED. . . . . . . . . . . . . . . . . . . . . . . . .51 3.2 ọkpọkọ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 nkọwa pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Nhazi pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Ihe nkwụnye ọkụ n'ọnụ Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Ntuli aka n'akụkụ akụkụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 Pipette sensọ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 Mmelite ngwa ngwa Pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 3.3 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 nkọwapụta gripper. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Gripper mmezi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Gripper emelite ngwa ngwa. . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 3.4 Pendant Kwụsị Mberede . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 Mgbe iji E-kwụsị . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 Na-etinye aka na ịtọhapụ E-kwụsị . . . . . . . . . . . . . . . . . . . . .60

3.5 Njikọ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 Njikọ ike. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 USB na njikọ inyeaka . . . . . . . . . . . . . . . . . . . . . . . . .62 Njikọ netwọk. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
3.6 nkọwa sistemu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63 nkọwapụta. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63 Nkọwapụta gburugburu ebe obibi. . . . . . . . . . . . . . . . . . . . . . . . . .64 Asambodo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 Oghere Usoro nọmba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64
Isi nke 4: Modul . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 4.1 modul akwadoro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66 4.2 Sistemụ caddy modul. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 4.3 Nhazi modul . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68 Mgbe a ga-ahazi modul. . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 Otu esi ahazi modul. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 4.4 Igwe ọkụ-Shaker Module GEN1 . . . . . . . . . . . . . . . . . . . . . . . . . .70 Heater-Shaker atụmatụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70 Nkọwapụta ọkụ-Shaker . . . . . . . . . . . . . . . . . . . . . . . . . .72 4.5 Igwe Ọdụdọ GEN1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 Magnetik Block atụmatụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 Nkọwapụta ngọngọ magnetik. . . . . . . . . . . . . . . . . . . . . . . . .74 4.6 Okpomọkụ Module GEN2. . . . . . . . . . . . . . . . . . . . . . . . . . . .75 Atụmatụ modul okpomọkụ. . . . . . . . . . . . . . . . . . . . . . . . .75 Nkọwapụta ọnọdụ okpomọkụ. . . . . . . . . . . . . . . . . . . .77 4.7 Thermocycler Modul GEN2. . . . . . . . . . . . . . . . . . . . . . . . . . . .77 Thermocycler atụmatụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .78 Nkọwa ọkụ ọkụ. . . . . . . . . . . . . . . . . . . . . . . . . . .79
Isi nke 5: Labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 5.1 Echiche labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Labware dị ka ngwaike . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Labware dị ka data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 omenala labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81 5.2 Mmiri mmiri. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Otu mmiri mmiri mmiri. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Multi-olulu mmiri. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Ebe nchekwa na nkọwa API. . . . . . . . . . . . . . . . . . . . . . . . 83 Custom reservoir labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 5.3 Efere nke ọma. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

6-efere nke ọma . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Efere 84 12-olulu mmiri. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Efere 84 24-olulu mmiri. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Efere 84 48-olulu mmiri. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Efere 84 96-olulu mmiri. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Efere 85 384-olulu mmiri. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 Ọfọn efere nkwụnye. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 Efere ọma na nkọwa API. . . . . . . . . . . . . . . . . . . . . . . . .87 omenala ọma efere labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 5.4 Ndụmọdụ na nkwakọba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 Nkwụnye ego. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 ndakọrịta Tippipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 Ntụpọ nkwụnye ọkụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 5.5 tubes na akwa ọkpọkọ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90 Ngwakọta tube na rack. . . . . . . . . . . . . . . . . . . . . . . . . . . 91 6-akwa akwa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 10-tube akwa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 15-akwa akwa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 24-tube akwa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Tube rack API nkọwapụta . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Omenala tube ogbe labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 5.6 Aluminom blocks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 Efere ala dị larịị. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 24-nke ọma aluminum ngọngọ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 96-nke ọma aluminum ngọngọ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 nkwụnye nkwụnye kwụ ọtọ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Nchikota aluminom ngọngọ labware. . . . . . . . . . . . . . . .94 24-ọmarịcha aluminom block labware nchikota. . . . . . . . .95 96-ọmarịcha aluminom block labware nchikota. . . . . . . . .95 5.7 Labware na Opentrons Flex Gripper . . . . . . . . . . . . . . .96 5.8 Nkọwa labware omenala. . . . . . . . . . . . . . . . . . . . . . . . . . . .96 Ịmepụta nkọwa labware omenala. . . . . . . . . . . . . . . . . . .97 JSON labware schema . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99 nkọwapụta labware JSON . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104 Isi nke 6: Protocol Development. . . . . . . . . . . . . . . . . . . . . . . . . . .105 6.1 Protocol emeburu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105 Protocol Library. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ọrụ mmepe Protocol omenala 105. . . . . . . . . . . . . . . .

6.2 Onye nrụpụta Protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109 Protocol Designer chọrọ. . . . . . . . . . . . . . . . . . . . . . 109 Ịmepụta protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Na-agbanwe ụkpụrụ ndị dị adị. . . . . . . . . . . . . . . . . . . . . . . . . . 114
6.3 Python Protocol API . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 Ederede ma na-agba ọsọ . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 Python-iche . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
6.4 OT-2 ụkpụrụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117 OT-2 Python protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118 OT-2 JSON protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120 Usoro modul magnetik. . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Isi nke 7: Akụrụngwa na Ọrụ. . . . . . . . . . . . . . . . . . . . . . . . . . 121 7.1 Touchscreen arụ ọrụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 Robot dashboard. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 njikwa protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122 nkọwa protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124 Gbaa ntọlite . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127 Nyochaa ọnọdụ ụlọ nyocha. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128 Na-agba ọsọ ọganihu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130 Na-agba ọsọ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131 njikwa akụrụngwa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132 Robot ntọala. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133 Nhazi oche. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136 7.2 Opentrons ngwa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 Ngwa nwụnye. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 Nyefee protocol na Flex. . . . . . . . . . . . . . . . . . . . . . . . . 140 modul na njikwa. . . . . . . . . . . . . . . . . . . . . . . . . . . 142 na nso nso a protocol na-agba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143 7.3 Ọrụ dị elu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Akwụkwọ ndetu Jupyter 144. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144 Ọrụ ahịrị iwu karịrị SSH. . . . . . . . . . . . . . . . . . . . 145
Isi nke 8: Nlekọta na Ọrụ . . . . . . . . . . . . . . . . . . . . . . . .147 8.1 Na-ehicha Flex gị. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Tupu ịmalite . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Ihe ị nwere ike ihicha . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Ngwọta nhicha. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148 Frame na windo panel ihicha. . . . . . . . . . . . . . . . . . . . 148 nhicha akwa akwa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148

Gantry nhicha . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 ihicha ụlọ mkpofu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 8.2 ihicha pipettes na ndụmọdụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 Pipette mkpochapụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150 ihicha ndụmọdụ pipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151 8.3 Na-ehicha ihe nkedo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152 8.4 modul nhicha. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 modul ihicha. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 Thermocycler akara. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 8.5 Autoclave-safe labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 8.6 Ọrụ Flex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 Opentrons ọrụ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 ntozu nwụnye na ntozu ọrụ. . . 155 Ndozi mgbochi . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156 Akwụkwọ ikike. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157 Ihe Okike A: Nkọwa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .158 Ihe Odide B: Mgbakwunye akwụkwọ . . . . . . . . . . . . . . . . . . . . . .170 B.1 Ebe Ọmụma Opentrons. . . . . . . . . . . . . . . . . . . . . . . . . . . . 170 B.2 Python Protocol API akwụkwọ . . . . . . . . . . . . . . . . . . . 170 B.3 Opentrons HTTP API ntụaka. . . . . . . . . . . . . . . . . . . . . . . . 171 B.4 Onye nrụpụta akwụkwọ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171 Ihe mgbakwunye C: Ngwanrọ mepere emepe. . . . . . . . . . . . . . . . . . . . . . . . .172 C.1 Opentrons na GitHub . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172 C.2 Opentrons monorepo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173 C.3 Ebe nchekwa ndị ọzọ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175 Ihe mgbakwunye D: Nkwado na ozi kọntaktị. . . . . . . . . . . . . . .176 D.1 Ahịa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 D.2 Nkwado . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 D.3 ozi azụmahịa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177

Okwu mmalite
Nnọọ na ntuziaka ntuziaka maka ngwa ngwa njikwa mmiri mmiri Opentrons Flex. Akwụkwọ ntuziaka a na-eduzi gị naanị ihe niile ịchọrọ ịma maka ịtọlite na iji Flex, na-elekwasị anya n'isiokwu ndị kachasị mkpa maka ndị ọrụ Flex kwa ụbọchị na gburugburu ụlọ nyocha.
Nhazi akwụkwọ ntuziaka a
Opentrons Flex bụ usoro dị mgbagwoju anya, yabụ enwere ọtụtụ ụzọ dị iche iche iji mụta ihe niile ọ nwere ike ime. Enwere onwe gị ịwụlikwa ozugbo gaa n'isiakwụkwọ na-ekwu maka isiokwu ọ bụla ị na-achọsi ike! Maka exampYa mere, ọ bụrụ na ịnweelarị Flex n'ime ụlọ nyocha gị, ị nwere ike ịgafe isi nwụnye na mbugharị.
Ọ bụrụ na-amasị gị ụzọ eduzi, a haziri akwụkwọ ntuziaka a ka ị nwee ike soro ya site na mmalite ruo ọgwụgwụ.
Mụta maka Flex. Edepụtara ihe dị iche iche nke Flex n'Isi nke 1: Okwu mmalite. Okwu mmeghe gụnyekwara ozi nchekwa dị mkpa na usoro iwu.
Bido na Flex. Ọ bụrụ na ịchọrọ ịtọlite Flex gị, soro ntuziaka zuru ezu n'Isi nke 2: Nwụnye na Mbugharị. Mara onwe gị nke ọma na akụkụ nke Flex na Isi nke 3: Nkọwa Sistemu.
Hazie oche gị. Ịhazi oche oche na-enyere ngwa sayensị dị iche iche aka na Flex. Isi nke 4: Modul na-akọwa oghere Opentrons nke ị nwere ike itinye n'ime ma ọ bụ n'elu oche iji rụọ ọrụ sayensị akọwapụtara. Isi nke 5: Labware na-akọwa otu esi arụ ọrụ na akụrụngwa maka ijide mmiri mmiri.
Gbaa usoro. Isi ojiji nke Flex bụ usoro sayensị ahaziri ahazi, nke a maara dị ka protocol. Isi nke 6: Protocol Development na-enye ụzọ dị iche iche iji nweta protocol emebere ma ọ bụ chepụta ha n'onwe gị. Iji mee protocol gị, soro ntuziaka dị n'Isi nke 7: Software na Operation, nke nwekwara ntuziaka maka ịrụ ọrụ ndị ọzọ na ịhazi ntọala robot gị.
Debe Flex na-agba ọsọ. Soro ndụmọdụ dị n'Isi nke 8: Nlekọta na Ọrụ ka Flex gị dị ọcha ma na-agba ọsọ nke ọma. Ma ọ bụ debanye aha maka otu ọrụ Opentrons edepụtara ebe ahụ ka anyị lekọta Flex maka gị.
Mụta ọbụna karịa. Ị ka chọrọ ihe ọzọ? Gaa na ngwa ngwa. Ihe mgbakwunye A: Nkọwa okwu na-akọwa okwu metụtara Flex. Ihe mgbakwunye B: Akwụkwọ mgbakwunye na-atụ gị aka na akụrụngwa ndị ọzọ maka ngwaahịa Opentron na koodu ederede iji jikwaa Flex.

OPENTRONS FLEX

MMADỤ
Ihe mgbakwunye C: Open-Source Software na-akọwa otu esi akwado ngwa ngwa Opentrons na GitHub dịka ihe enyemaka maka ma ndị mmepe na ndị na-abụghị ndị mmepe.
Ihe mgbakwunye D: Nkwado na ozi kọntaktị depụtara otu esi enweta kọntaktị na Opentron ma ọ bụrụ na ịchọrọ enyemaka karịrị ihe akwụkwọ anyị nyere.
Ihe ndetu na ịdọ aka ná ntị
N'ime akwụkwọ ntuziaka a, ị ga-ahụ ndetu ahaziri ahazi na mgbochi ịdọ aka ná ntị. Ihe ndetu na-enye ozi na-enye aka nke nwere ike ọ gaghị apụta ìhè n'ụzọ nkịtị nke iji Flex. Lezienụ anya na ịdọ aka ná ntị pụrụ iche-a na-eji ha naanị n'ọnọdụ ebe ị nwere ihe ize ndụ nke mmerụ onwe gị, mmebi nke akụrụngwa, mfu ma ọ bụ mmebi nke s.amples ma ọ bụ reagents, mfu data, ma ọ bụ ihe ọjọọ ndị ọzọ. Ihe ndetu na ịdọ aka ná ntị dị ka nke a:
SampRịba ama: Nke a bụ ihe ị kwesịrị ịma, mana ọ nweghị ihe egwu ọ bụla.
Sample Ịdọ aka ná ntị: Nke a bụ ihe i kwesịrị ịma n'ihi na e nwere ihe ize ndụ metụtara ya.

OPENTRONS FLEX

ISI NKE 1
Okwu mmalite

Isiakwụkwọ a na-ewebata gị na sistemụ gburugburu ebe obibi Opentrons Flex, gụnyere nhazi sistemu n'ozuzu yana nhazi ebe arụ ọrụ dị. Ọ gụnyekwara nrubeisi na ozi nchekwa dị mkpa, nke ị ga-emegharịview tupu ịtọlite robot Opentrons Flex gị. Maka nkọwa ndị ọzọ gbasara njirimara Opentrons Flex, lee isi nkọwa nkọwa.

1.1 Nabata na Opentrons Flex
Opentrons Flex bụ robot na-ejikwa mmiri emebere maka ntinye dị elu yana usoro ọrụ siri ike. Robot Flex bụ ntọala nke sistemu modular na-agụnye pipettes, ihe nrụnye labware, ihe ndozi oche, modul n'elu oche, na labware - ihe niile ị nwere ike ịgbanwe onwe gị. Emebere Flex na ihuenyo mmetụ ka ị nwee ike soro ya rụọ ọrụ ozugbo na bench ụlọ nyocha, ma ọ bụ ị nwere ike ijikwa ngwa Opentrons ma ọ bụ API mepere emepe.
Ebe a na-arụ ọrụ Flex na-eji akụrụngwa niile - robot, ngwaike, na labware - nke ịchọrọ ịmalite ịmezi ọrụ ụlọ nyocha. Maka ngwa ndị ọzọ, Opentrons Flex na-agba ọsọ na ngwanrọ mepere emepe yana ngwa ngwa, yana reagent na labware-agnostic, na-enye gị njikwa otu esi emepụta na hazie usoro gị.

Kedu ihe dị ọhụrụ na Flex
Opentrons Flex bụ akụkụ nke usoro robots nke Opentrons mmiri mmiri. Ndị na-eji Opentrons Flex nwere ike mara Opentrons OT-2, rọbọt ọkpọ ọkpọ anyị. Flex na-agabiga ikike nke OT-2 n'ọtụtụ mpaghara isi, na-ebuga ntinye dị elu na oge njem.

Njirimara Pipette ntinye
Pipette na n'ọnụ ike

Nkọwa
Flex pipettes nwere ọwa 1, 8 ma ọ bụ 96. Pipette nke ọwa 96 na-arụ ọrụ ugboro 12 karịa ọtụtụ olulu mmiri n'otu oge dị ka pipette OT-2 kacha ukwuu.
Flex pipettes nwere oke olu buru ibu (1 µL, 50 µL) na ha niile nwere ike iji olu ọ bụla nke ndụmọdụ Opentrons Flex rụọ ọrụ. Nke a bụ nkwalite karịa pipettes OT-5, nke nwere oke dị nta ma ga-eji ndụmọdụ nwere oke ụda dabara adaba.

OPENTRONS FLEX

ISI NKE MBU: NKE MBU

Gripper Automated calibration Touchscreen Module caddies Deck slot na-ahazi nha ahịhịa na ịdị arọ

Opentrons Flex Gripper na-ebuli ma bugharịa labware gburugburu oche ahụ na-akpaghị aka, na-enweghị enyemaka onye ọrụ. The gripper na-enyere aka mgbagwoju workflows n'ime otu protocol ọsọ.
Nhazi ọkwa nke Flex pipettes na gripper bụ akpaaka zuru oke. Pịa otu bọtịnụ, ngwa ahụ ga-aga n'ihu na-arụ ọrụ nkenke n'elu oche iji chọpụta kpọmkwem ọnọdụ ya, na-echekwa data ahụ maka ojiji na usoro gị.
Flex nwere interface mmetụ aka ya nke na-enye gị ohere ijikwa ya ozugbo, na mgbakwunye na iji ngwa Opentrons. Jiri ihuenyo mmetụ ahụ ka ịmalite ịgba ọsọ protocol, lelee ọkwa ọrụ, wee gbanwee ntọala ozugbo na robot.
Modul Flex dabara na caddies na-ejide oghere n'okpuru oche ahụ. Caddies na-edobe labware gị nso n'elu oche ma kwe ka ị na-ebugharị eriri USB n'okpuru oche. Caddies na-eme ka modul na nhazi labware karịa na oche ahụ.
A na-agụ oghere oche na Flex na sistemụ nhazi (A1D4) nke yiri ka esi agụ olulu mmiri na labware.
Ihe mkpofu ahịhịa nwere ike ịga n'ọtụtụ ebe nchekwa na Flex. Ebe ndabara (oghere A3) bụ ọnọdụ akwadoro. Ị nwekwara ike iji gripper tụfuo ahịhịa n'ime ụlọ mkpofu nhọrọ.
Flex dị ntakịrị ma dị arọ karịa OT-2. Ọrụ ntinye na Flex chọrọ enyemaka nke onye mmekọ ụlọ nyocha.

Ntụle zuru ezu nke nkọwa teknụzụ robot dị na Opentrons websaịtị.
Ma Flex na OT-2 robots na-agba ọsọ na ngwanrọ mepere emepe anyị, na Opentrons App nwere ike ijikwa ụdị rọbọtụ abụọ n'otu oge. Ọ bụ ezie na OT-2 enweghị ike ịgba ọsọ ozugbo na Flex, ọ dị mfe imeghari ha (lee ngalaba OT-2 Protocols nke isi Protocol Development maka nkọwa).

Ụlọ ọrụ Flex
Ụlọ ọrụ Opentrons Flex gụnyere Flex robot, ngwa, pipettes na gripper, modul n'elu oche, na labware achọrọ iji megharịa otu ngwa. Ngwa niile arụ ọrụ bụ modular. Ọ bụrụ na ịchọrọ ịgbanwe ngwa, ịnwere ike ịgbakwunye ma ọ bụ gbanwee na ngwaike Flex ndị ọzọ yana ngwa ahịa dakọtara.

OPENTRONS FLEX

ISI NKE MBU: NKE MBU
Ụlọ ọrụ NGS
Ụlọ ọrụ Opentrons Flex NGS na-arụ ọrụ nkwadobe ọba akwụkwọ NGS. Ọ nwere ike rụọ ọrụ usoro nhazi nke mbụ site na iji sistemụ reagent ọ bụla na-eduga, gụnyere nkewa- na tagNkwadebe n'ọbá akwụkwọ dabere n'aha.
Na mgbakwunye na robot Flex, NGS Workstation gụnyere:
Nhọrọ Gripper nke nhazi pipette
Ọwa 8-Channel Pipettes abụọ (1 µL na 50 µL) 5-Channel Pipette (1000 µL) Waste Chute Magnetic Block Temperature Module Thermocycler Module Labware kit na ndụmọdụ nzacha, microcentrifuge tubes, reservoirs, na PCR.
PCR ọrụ
Ụlọ ọrụ Opentrons Flex PCR na-arụ ọrụ nhazi PCR na ọrụ ọkụ ọkụ ruo 96 s.amples. Ọ nwere ike iwepụta reagents jụrụ oyi na samples n'ime efere PCR nke ọma 96. Na mgbakwunye na Modul Thermocycler akpaghị aka, jiri gripper bunye efere n'ime Thermocycler, wee mee mmemme PCR ị họọrọ.
Na mgbakwunye na robot Flex, PCR Workstation gụnyere:
Nhọrọ Gripper nke nhazi pipette
1-Channel Pipette (1 µL) na 50-Channel Pipette (8 µL) 1-Channel Pipette (50 µL) Waste Chute Temperature Module Labware kit na ndụmọdụ nzacha, microcentrifuge tubes, reservoirs, na PCR efere.

OPENTRONS FLEX

ISI NKE MBU: NKE MBU
NUCLEIC ACIID ỌRỤ ỌRỤ
Opentrons Flex Nucleic Acid Extraction Workstation na-arụ ọrụ ikewapụ DNA/RNA na ịdị ọcha. Ọ na-eji Magnetic Block maka nkewa nke ọla mma magnetik, yana Heater-Shaker maka s.ample lysis na resuspension nke magnetik beads.
Na mgbakwunye na robot Flex, Nucleic Acid Extraction Workstation gụnyere:
Nhọrọ Gripper nke nhazi pipette
1-Channel Pipette (5 µL) na 1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL) Waste Chute Magnetic Block Heater-Shaker Module Labware kit na nzacha Atụmatụ, reservoirs, PCR efere ọma, na miri emi efere.
ỌRỤ ỌRỤ MMANTIC Bead Protein dị ọcha
Ụlọ ọrụ Opentrons Flex Magnetic Bead Protein Purification Workstation na-arụ ọrụ ọcha nke obere protein na proteomics s.ample njikere maka ihe ruru 96 samples. Ọ dakọtara na ọtụtụ reagents dabere na magnetik ama ama.
Na mgbakwunye na robot Flex, Protein Purification Workstation gụnyere:
Nhọrọ Gripper nke nhazi pipette
1-Channel Pipette (5 µL) na 1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL) Waste Chute Magnetic Block Heater-Shaker Module Labware kit na nzacha Atụmatụ, reservoirs, PCR efere ọma, na miri emi efere.
FLEX PREP ỌRỤ
Ụlọ ọrụ Opentrons Flex Prep na-arụ ọrụ na-arụ ọrụ pipetting dị mfe. Hazie ebe a na-arụ ọrụ na 1-Channel na 8-Channel Pipettes iji rụọ ọrụ dịka s.ample mbufe, sample mbiputegharị, na

OPENTRONS FLEX

ISI NKE MBU: NKE MBU
ihe na-eme reagent. Hazie ebe a na-arụ ọrụ na 96-Channel Pipette iji rụpụta reagent aliquoting dị elu na efere efere.amping.
Na mgbakwunye na Flex robot, Flex Prep Workstation gụnyere:
Nhọrọ nke nhazi pipette: 1-Channel Pipette (5 µL) na 1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL)
Ngwa labware nwere ndụmọdụ nzacha, ọkpọkọ microcentrifuge, na ebe nchekwa
ỌRỤ Nkwadebe PlaSMID
Ụlọ ọrụ Opentrons Flex Plasmid Prep na-arụ ọrụ na-arụ ọrụ plasmid nke dabere na magnetik na nchacha. Ejiri pipette dị elu rụọ ọrụ a, Module Heater-Shaker, yana Block Magnetic iji nabata kemistri dabere na ọtụtụ.
Na mgbakwunye na robot Flex, Plasmid Prep Workstation gụnyere:
Gripper 1-Channel Pipette (5 µL) na 1000-Channel Pipette (8 µL) Waste Chute Magnetic Block Heater-Shaker Module Labware kit nwere ndụmọdụ nzacha, obere microcentrifuge tubes, reservoirs, PCR efere, na miri ọma efere.
Ụlọ ọrụ SYNBIO
Ụlọ ọrụ Opentrons Flex SynBio na-arụ ọrụ dịgasị iche iche nke usoro ihe ọmụmụ ihe dị ka DNA njikọ na cloning. Ọ na-eji Magnetic Block na Temperature Module iji kwado ọtụtụ kemistri dabere na bead. Tinye Thermocycler Module ka ịrụ mkpuchi mkpuchi na-ekpo ọkụ na ampọkụ ọkụ.
Na mgbakwunye na robot Flex, SynBio Workstation gụnyere:
Gripper 1-Channel Pipette (5 µL) na 1000-Channel Pipette (8 µL) Igwe Ọdụdọ Igwe Igwe Igwe Ọdụdọ

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ISI NKE MBU: NKE MBU

Ngwa labware nwere ndụmọdụ oge niile na nzacha, ọkpọkọ microcentrifuge, mmiri mmiri, efere PCR, na efere olulu mmiri miri emi.

1.2 Ozi nchekwa
Emebere igwe mmiri mmiri Opentrons Flex maka ịrụ ọrụ nchekwa. Rụtụ aka na nkọwapụta na ụkpụrụ nnabata dị na ngalaba a iji hụ na iji Flex gị eme ihe n'enweghị nsogbu. Ntuziaka ndị a na-ekpuchi iji nchekwa ntinye na njikọ mmepụta maka ngwaahịa ahụ, gụnyere ike na njikọ data, yana akara ịdọ aka ná ntị achọtara na Flex robot na ngwaike metụtara ya. Iji ngwaọrụ n'ụzọ na-abụghị nke akọwapụtara n'akwụkwọ ntuziaka a nwere ike itinye onye ọrụ na akụrụngwa n'ihe egwu.

Akara nchekwa
Akara aha dị iche iche na Flex na n'akwụkwọ ntuziaka a na-adọ gị aka na ntị maka isi mmalite nke mmerụ ahụ ma ọ bụ mmerụ ahụ.

Akara

Nkọwa
Ịdọ aka ná ntị: Na-eme ka ndị ọrụ mara ọnọdụ nwere ike ịdị ize ndụ. Omume ndị nwere ike ibute mmerụ ahụ ma ọ bụ ọnwụ nke onwe.
Akpachara anya: dọọ ndị ọrụ aka na ntị megide mmebi nke akụrụngwa. Data furu efu ma ọ bụ mebie. Nkwụsị ọrụ a na-eme na-enweghị mgbake.
Igwe ọkụ eletrik: Na-achọpụta ihe akụrụngwa nwere ike ibute ihe egwu ọkụ eletrik ma ọ bụrụ na ejiri ya nke ọma.

Elu na-ekpo ọkụ: Na-achọpụta ihe akụrụngwa na-ebute ihe ize ndụ nke mmerụ onwe onye n'ihi oke okpomọkụ / okpomọkụ ma ọ bụrụ na ejizi ngwaọrụ ahụ n'ụzọ na-ekwesịghị ekwesị.

Pinch point: Na-achọpụta akụrụngwa akụrụngwa nwere ike ibute ihe egwu nke mmerụ ahụ mgbe ọ na-emegharị.

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Ị ga-ahụ akara ndị a na Flex:
Mpempe akwụkwọ ikike ọgụgụ isi Akara nrube isi nke usoro iwu (dịka ọmụmaatụ, ETL) akara egwu ọkụ eletrik Akara ngosi ịdọ aka na ntị izugbe Akara ngwaahịa Pinch point labels High voltage na-ede aha akara ọkwa ike

Ịdọ aka ná ntị nchekwa eletrik
Na-edebe ịdọ aka ná ntị nchekwa eletrik ndị a mgbe niile:

Akara

Nkọwa
Tinye robot n'ime sekit nke klaasị 1. Hụ ngalaba Njikọ Ike na isi nkọwa Sistemu.

Ejikọtala (ntinye), kwupu (wepu) ma ọ bụ jiri eriri ọkụ AC ma ọ bụrụ: Ejijiri eriri ma ọ bụ mebie. Igwe, eriri, ma ọ bụ ihe nkwụnye ego ndị ọzọ agbakwunyere na-agbaji ma ọ bụ mebie.
Iji eriri ọkụ mebiri emebi nwere ike ibute ihe egwu ọkụ eletrik na-ebute mmerụ ahụ dị egwu ma ọ bụ mebie robọt.
Edochiela eriri ọkụ AC ọ gwụla ma na ntụzịaka nke Nkwado Opentrons.

Maka ozi ndị ọzọ gbasara ihe ọkụ eletrik chọrọ, lee ngalaba oriri ike nke isi nwụnye na mbugharị.

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Ịdọ aka ná ntị nchekwa ndị ọzọ
Debe ịdọ aka ná ntị nchekwa ndị a mgbe niile:

Akara

Nkọwa
Opentrons Flex enwetabeghị asambodo maka ojiji ya na mmiri mgbawa ma ọ bụ ọkụ. Ejila efere, tubes, ma ọ bụ vial nwere ihe mgbawa ma ọ bụ ọkụ ọkụ n'ime robọt ma ọ bụ jiri ihe mgbawa ma ọ bụ ihe na-ere ọkụ rụọ ọrụ n'ime ogige ahụ.
Jiri ezigbo ụlọ nyocha wee soro kpachara anya nke ndị nrụpụta mgbe ị na-arụ ọrụ na kemịkalụ. Opentrons anaghị akwụ ụgwọ ma ọ bụ kwụọ ụgwọ maka mmebi ọ bụla n'ihi, ma ọ bụ n'ihi, iji kemịkal dị ize ndụ.

Flex dị kilogram 88.5 (195 lbs). N'ihi ya, ọ na-achọ mmadụ abụọ ka ha bulie ma bugharịa ya n'enweghị nsogbu. Hụ ngalaba Mbugharị na isi nwụnye na mbugharị.

Ekwesịrị idowe Flex n'elu elu nke nwere ike ịkwado ịdị arọ ya nke 88.5 kg (195 lbs) na mpaghara elu zuru oke iji nabata robot gbakwunyere nkwụsị kacha nta (20 cm/8 in). Hụ ngalaba nchekwa na ọrụ chọrọ n'isi nke nwụnye na mbugharị.
Flex nwere ike ibunye ịma jijiji mgbe ọ na-arụ ọrụ. Tinye robot n'elu nke siri ike, dị larịị, na mmiri na-eguzogide ya na ihe nkwado ma ọ bụ nkwonkwo welded. Hụ ngalaba nchekwa na ọrụ chọrọ n'isi nke nwụnye na mbugharị.

Akpachara anya maka nchekwa
Iji nyere aka chekwa Flex site na mmebi, soro akpachapụ anya ndị a:

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Akara

Nkọwa
Jiri labware dabara ANSI/SLAS ma ọ bụ nke Opentron kwadoro. Hụ isi Labware.
Debe ihe ndị na-emebi emebi, ihe ndị na-emebi emebi, ma ọ bụ ihe ndị ọzọ na-emebi ihe n'ebe robot ahụ dị.

Nchekwa ndu
Mesoo ihe nlere na reagents nwere akụrụngwa ewepụtara n'aka mmadụ dị ka ihe nwere ike ibute ọrịa. Opentrons na-atụ aro iji usoro ụlọ nyocha dị mma dịka akọwara na Biosafety in Microbiological and Biomedical Laboratories (BMBL) Mbipụta nke isii.
N'ọnọdụ nkịtị, Flex anaghị emepụta ikuku ikuku nwere ike ịchọpụta site na mmiri mmiri. Otú ọ dị, n'okpuru ọnọdụ ụfọdụ, ọ ga-ekwe omume ịmepụta aerosols site na mmiri mmiri mmiri. Mgbe ị na-arụ ọrụ na ọkwa biosafety 2 ma ọ bụ mmiri mmiri ka ukwuu, tụlee ịkpachara anya megide ikpughe ikuku aerosol, dịka ndị ọrụ na-ahụ maka mpaghara gị siri dị. Iji belata ihe ize ndụ nke ikpughe ikuku aerosol site na robot, hụ na ị:
Mee mmezi dị ka akọwara n'isi nke mmezi na ọrụ. Wụnye nke ọma ma chekwaa mkpuchi ngwá ọrụ niile, pipettes, modul na labware. Jiri usoro ọkpọkọ kwesịrị ekwesị nyere aka n'ibelata ikuku ikuku.
Uzuzu na-egbu egbu
Ọ bụrụ na ị na-eji ihe mgbaze na-agbanwe agbanwe ma ọ bụ ihe ndị na-egbu egbu na-arụ ọrụ, jiri sistemu ikuku ventilashị nke ọma wee wepụ vapors ọ bụla enwere ike imepụta.
Mmiri mmiri ọkụ
Atụlela Flex maka iji mmiri ọkụ na-ere ọkụ ma e kwesịghị iji mmiri ọkụ na-ere ọkụ.

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1.3 Nrube isi n'usoro
Opentrons Flex na-akwado ihe niile achọrọ maka nchekwa na ụkpụrụ elektrọn.

Nchekwa
Iwu ID IEC/UL/CSA 61010-1 IEC/UL/CSA 61010-2-051

Aha
Ihe nchekwa nchekwa maka ngwa eletriki maka nha, njikwa na ojiji ụlọ nyocha Nkebi 1: Ihe ndị achọrọ n'ozuzu
Ihe achọrọ pụrụ iche maka akụrụngwa ụlọ nyocha maka ịgwakọta na ịkpali

Ndakọrịta elektrọnik

Iwu ID EN/BSI 61326-1
FCC 47 CFR Nkebi 15 Nkebi B Klas A IC ICES-003

Aha
Ngwa eletriki maka nha, njikwa na ụlọ nyocha jiri ihe EMC chọrọ Nkebi 1: Ihe ndị achọrọ n'ozuzu radiators na-amaghị ama.
Njikwa Spectrum na nnyonye anya nzikọrịta ozi na-ebute ngwa akụrụngwa ọkọlọtọ teknụzụ teknụzụ ozi (gụnyere ngwa dijitalụ)

FCC ịdọ aka ná ntị na ndetu
Ịdọ aka ná ntị: Mgbanwe ma ọ bụ mgbanwe na ngalaba a nke Opentrons anabataghị nke ọma nwere ike imebi ikike onye ọrụ iji rụọ ọrụ ahụ.
Ngwaọrụ a na-akwado akụkụ 15 nke iwu FCC. A na-arụ ọrụ n'okpuru ihe ndị a:

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Ngwaọrụ a nwere ike ọ gaghị ebute nnyonye anya na-emerụ ahụ. Ngwaọrụ a ga-anabatarịrị nnyonye anya ọ bụla enwetara, gụnyere nnyonye anya nke nwere ike ibute enweghị isi
ọrụ.
Mara: A nwalere ngwa a wee hụ na ọ dabara na oke maka ngwaọrụ dijitalụ klaasị A, dabere na akụkụ 15 nke iwu FCC. Emebere oke ndị a iji nye nchebe ezi uche dị na ya pụọ na nnyonye anya na-emerụ emerụ mgbe ejiri akụrụngwa na mpaghara azụmahịa. Akụrụngwa a na-ewepụta, na-eji, ma nwee ike ịgbasa ike ugboro redio yana, ọ bụrụ na etinyeghị ya ma jiri ya dịka ntuziaka ntuziaka siri dị, nwere ike ibute ndabichi na-emerụ ahụ na nkwukọrịta redio. Ịrụ ngwa a n'ime ebe obibi nwere ike ịkpata nnyonye anya na-emerụ ahụ nke a ga-achọ ka onye ọrụ dozie nnyonye anya na ego nke aka ha.
Canada ISED nnabata
Canada ICES-003(A) / NMB-003(A)
Ngwaahịa a zutere nkọwa nka nka nke Innovation, Science and Economic Development Canada.
Usoro nkọwapụta nke ọma ga-emetụta d'Innovation, Sciences et Développement economique Canada.
Ịdọ aka ná ntị gburugburu ebe obibi
Ịdọ aka ná ntị: Ọrịa cancer na mmempụ ịmụ nwa www.P65Warnings.ca.gov
Asambodo Wi-Fi
Edoziri modul Wi-Fi maka ojiji n'ọtụtụ mpaghara:
United States (FCC): FCC Identifier UAY-W8997-M1216 European Economic Area (CE): Enweghị njirimara ọha (nkwupụta onwe) Canada (IC): Nọmba Nchọpụta Ngwaike W8997-M1216 Japan (TELEC): Nọmba akwadoro 020-170034 India (WPC): Nọmba ndebanye aha ETA-SD-20191005525 (nkwupụta onwe)

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ISI NKE 2
Nwụnye na mbugharị
Isiakwụkwọ a na-akọwa otu esi akwado ụlọ nyocha gị maka Opentrons Flex, otu esi edozi rọbọt, yana otu esi ebugharị ya ma ọ dị mkpa. Tupu ibuga Flex gị, jide n'aka na ụlọ nyocha gị ma ọ bụ ụlọ ọrụ gị na-emezu ihe niile dị na ngalaba nchekwa na ọrụ chọrọ. Mgbe oge ruru iji bulie Flex gị ma na-agba ọsọ, soro ntuziaka zuru ezu na ngalaba Unboxing, Run Run, na Instrument Installation and Calibration, ma ọ bụ jiri Opentrons Onsite Support Set Up service. Ma ọ bụrụ na ịchọrọ ịkwaga Flex gị gaa ebe ọhụrụ, nso ma ọ bụ dị anya, soro usoro ndị dị na ngalaba Mbugharị.
2.1 Nchekwa na ọrụ chọrọ
Ebe ị ga-edebe Opentrons Flex
Oghere bụ ihe bara uru n'ihe fọrọ nke nta ka ọ bụrụ ụlọ nyocha ọ bụla. Flex gị ga-achọ ụfọdụ-ma ọ bụghị nke ukwuu, ebe e mere ya ka ọ dabara na ọkara nke ụlọ nyocha ụlọ nyocha. Jide n'aka na ị nwere oghere na-emezu ụkpụrụ ndị a.
N'elu oche: Ọ kwụ ọtọ, siri ike, ọkwa, elu mmiri na-eguzogide ọgwụ. A naghị atụ aro tebụl ma ọ bụ oche nwere wiil (ọbụlagodi mkpuchi mkpuchi). Flex na-aga ngwa ngwa ma nwee ọtụtụ oke, nke nwere ike ịma jijiji ma ọ bụ mee ka tebụl dị fechaa ma ọ bụ nke mbughari.
Ibu ibu: Robot naanị ya ruru kilogram 88.5 (195 lb) na naanị mmadụ abụọ na-arụkọ ọrụ ga-ebuli ya. Tinye robot n'elu nke nwere ike ịkwado ibu ya ngwa ngwa gbakwunyere ibu nke modul ọ bụla, labware, mmiri mmiri, ma ọ bụ ngwa ụlọ nyocha ndị ọzọ a ga-eji na ngwa gị.
Oghere eji arụ ọrụ: Akụkụ ntọala robot bụ 87 cm W x 69 cm D x 84 cm H (ihe dịka 34 ″ x 27″ x 33″). Flex chọrọ 20 cm (8 ″) nke akụkụ na azụ azụ maka eriri, njikọ USB, na ikpochapụ ikuku sitere na modul na-ekpo ọkụ ma dị jụụ.
Ịdọ aka ná ntị: Edoghachila akụkụ ma ọ bụ azụ nke Flex na-asọba na mgbidi.

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84 cm 33 "

87 cm 34 "
Akụkụ ntọala Opentrons Flex.

69 cm 27 "

20 cm 8 "

N'elu view nke Opentrons Flex, na-egosi njedebe kacha nta na akụkụ azụ.

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Oriri ike
Ekwesịrị ijikọ Opentrons Flex na oghere mgbidi na ma ọ bụ nso ebe oche ebe ị rụnyere ya. Naanị jikọọ Flex na sekit nwere ike ịnabata ihe osise kacha ike:
Ike ntinye: 36 VDC, 6.1 A oriri na-adịghị arụ ọrụ: 30 W Oriri a na-ahụkarị: 40 W (n'oge usoro protocol) Oriri kachasị: Ihe dịka 50 W
Oriri ike dabere na:
Ọnụ ego na ụdị mmegharị ahụ a na-eme n'oge protocol. Ogologo oge robọt na-etinye n'ọrụ. Ọnọdụ nke ọkụ na robot. Ngwa ole agbakwunyere.
Cheta na ị ga-aza ajụjụ maka ngwa eletrọnịkị ndị ọzọ na-eri ike n'otu sekit, gụnyere Flex modul nwere ike nke ha. Maka exampLe, Thermocycler Module nwere oke ike oriri (630 W) nke dị ukwuu karịa Flex robot n'onwe ya. Ọ bụrụ na ọ dị mkpa, kpọtụrụ onye njikwa ụlọ ọrụ gị ka ị hụ na ọ dabara ihe achọrọ ike akụrụngwa gị.

Ọnọdụ gburugburu ebe obibi
Ọnọdụ gburugburu maka ojiji akwadoro, ojiji anabatara na nchekwa dị iche iche:

Akwadoro maka ịrụ ọrụ sistemụ

A na-anabata maka ịrụ ọrụ sistemụ

Okpomọkụ ikuku sitere na +20 ruo +25 Celsius

+2 ruo +40 Celsius

Ọdịda iru mmiri dị elu

40%, enweghị condensing
Ihe dị ka 500 m n'elu oke osimiri

30%, enweghị condensing (n'okpuru 80 Celsius)
Ruo 2000 m n'elu oke osimiri

Nchekwa na ụgbọ njem
-10 ka +60 Celsius
10%, enweghị condensing (n'okpuru 85 Celsius)
Ruo 2000 m n'elu oke osimiri

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Opentrons akwadola arụmọrụ Opentrons Flex na ọnọdụ akwadoro maka ịrụ ọrụ sistemu, yana ịrụ ọrụ na ọnọdụ ndị ahụ kwesịrị inye nsonaazụ kacha mma. Flex adịghị mma iji na ọnọdụ anabatara maka ịrụ ọrụ sistemụ, mana nsonaazụ nwere ike ịdị iche. Agbanyela ma ọ bụ jiri Flex na ọnọdụ na-abụghị oke ndị ahụ. Ọnọdụ nchekwa na njem na-emetụta naanị mgbe robot kwụsịrị kpamkpam na ike na akụrụngwa ndị ọzọ.
2.2 Unboxing
Ekele! Opentrons Flex gị abatala ma ị kwadola oghere maka ya n'ụlọ nyocha gị. Ka anyị mepee igbe anụ ahụ, wepụ robọt, ma kwado ya maka ịrụ ọrụ. Ozi dị na ngalaba a na-enye ndepụta akụkụ na ntuziaka na-ejegharị gị site na usoro achọrọ iji mee ka Flex enweghị igbe, hazie ma dị njikere maka ojiji. Anyị ekewala usoro nhazi ahụ ụzọ atọ:
Akụkụ nke 1 na-ekpuchi ịkwasa crate. Akụkụ nke 2 na-ekpuchi iwepụ Flex na crate ma bugharịa ya na ebe mgbakọ ikpeazụ. Akụkụ 3 na-ekpuchi mgbakọ ikpeazụ na ike na robot maka oge mbụ.
Mgbalị na oge achọrọ
Ị ga-achọ ịrịọ onye gị na ya na-arụkọ ọrụ ụlọ nyocha ka ọ nyere aka na ịgbapụ, ibuli elu, ịkwagharị na usoro mgbakọ. Ị ga-achọ ịhazi ihe dị ka nkeji 30 ruo otu awa maka mbọ a.
Mara: Flex chọrọ mmadụ abụọ ka ebuli ya nke ọma. Ọzọkwa, ibuli na iburu Flex site na aka ya bụ ụzọ kacha mma isi bugharịa roboti.
Crate na nkwakọ ngwaahịa
Iwepu Flex na-enye gị robot dị egwu, mana ị ga-ahapụkwa ọtụtụ nnukwu oghere yana ngwa mbupu dị iche iche na padding. Mgbe ị nwere ike ịtụfu ihe a, anyị na-agba gị ume idobe ihe ndị a ma ọ bụrụ na ohere nchekwa dị. Enwere ike ịmegharị nkwakọ ngwaahịa ahụ, nke na-enyere aka ịkwadebe Flex gị maka mbupu ma ọ bụrụ na ịchọrọ iziga ya ebe ọzọ (dịka ọmụmaatụ, na ogbako ma ọ bụ ụlọ ọhụrụ) n'ọdịnihu.

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Ngwa ngwaahịa
Ụgbọ mmiri Flex nwere ihe ndị e depụtara n'okpuru. Pipettes, gripper na modul na-abata na nkwakọ ngwaahịa dị iche iche site na nnukwu oghere Flex, ọbụlagodi na ị zụtara ha ọnụ dịka ebe a na-arụ ọrụ.

(1) Opentrons Flex robot

(1) eriri USB

(1) Igwe ọkụ

(1) eriri Ethernet

(5) igodo L (hex 12 mm, hex 1.5 mm, hex 2.5 mm, hex 3 mm,
T10 Torx)

(1) Pendanti Kwụsị Mberede

(1) Oghere oche nwere mkpachị labware

(4) Mpempe akwụkwọ mpempe akwụkwọ mapụtara

(1) Nchọpụta nhazi nke pipette

(4) Ibu aka na okpu

(1) Ogwe windo dị elu

(4) Oghere windo akụkụ

(1) 2.5mm hex screwdriver

(1) 19 mm mgbakwụnye

(16+ mapụtara) kposara windo (isi larịị M4x8 mm)
26

(10) skru oghere oghere mapụtara (isi oghere M4x10 mm)

(12) skru mkpirisi mkpirisi mapụtara (isi oghere M3x6 mm)

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU
Nkebi nke 1: Wepu igbe ahụ
Opentrons na-ebufe Flex gị n'ime akpa plywood siri ike. Igwe mbupu na-eji nko na latch clamps iji chekwaa akụkụ elu, akụkụ na ala ọnụ. Iji latches, kama ntu ma ọ bụ skru, pụtara na ị gaghị achọ crowbar (ma ọ bụ ike dị ukwuu) iji chịkọta akwa ahụ, ma ị nwere ike ịkwakọta ya ma emesịa, ọ bụrụ na ọ dị mkpa.
Mara: Akụkụ crate nwere ike ịgbatị elu n'oge mbupu. Ị nwere ike iji gloves ọrụ iji chebe aka gị pụọ na mgbawa osisi.
Ka ịtọhapụ latches, tụgharịa taabụ latch elu wee tụgharịa gaa n'aka ekpe (akụkụ elekere). Omume a na-akpali clamp ogwe aka pụọ na njigide njigide kwekọrọ. Ị nwere ike wee tụgharịa ogwe aka nkedo ahụ pụọ na crate.
1 Mepee latches asatọ na-ejide elu n'akụkụ.

2 Wepu akụkụ elu ka ahapụchara latches.

OPENTRONS FLEX

ISI NKE 2: Nwụnye na Mbugharị 3 Mepee akpa mbupu na-acha anụnụ anụnụ, wepụ ihe ndị a na padding, ma debe ha n'akụkụ:
Ike ngwa onye ọrụ, Ethernet, na eriri USB Mkwụsị ihe mberede
4 Wepu mpempe ụfụfụ dị n'elu ka ikpughe akụkụ windo. Ihe mgbochi na-echebe akụkụ na akụkụ elu.
5 Wepu akụkụ windo ma debe ha n'akụkụ. Ị ga-agbakwunye ndị a ma emechaa.

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU
6 Mepee oghere 16 fọdụrụnụ na-ejide akụkụ akụkụ na ibe ya na ala nke crate. 7 Wepu akụkụ akụkụ ma debe ha n'akụkụ.
Akụkụ 2: Hapụ Flex
Mgbe ịmechara usoro ndị dị na Nkebi nke 1, ị ga-ahụ ugbu a robot nke dị na akpa nchekwa ma tinye ya na ihe mgbakwasị nchara oroma. Akpa ahụ na-ekpuchi robot ma chebe ya pụọ na gburugburu ebe dị n'èzí. Ihe nkwado ígwè na-echekwa robọt ahụ na ala nke igbe ahụ. Oghere mbupu abụọ na-akwado rọbọt, na-ekesa ịdị arọ ya nke ọma, na idobe ya nke ọma ka ọ ghara ịgbagha n'oge mbupu. Gaa n'ihu ịtọpụ Flex ma wepụ ya n'ime oghere.
8 Iji mgbanaka 19 mm site na ngwa onye ọrụ, wepụ ihe nkwado ahụ site na crate ala. Ị nwere ike tụfuo brackets, ma ọ bụ chekwaa ha maka ojiji n'ọdịnihu.

OPENTRONS FLEX

ISI NKE 2: Nwụnye na Mbugharị 9 Dọrọ ma ọ bụ tụgharịa akpa mbupu ruo ala ka ikpughe robot niile.
10 Site n'enyemaka site n'aka onye gị na ya na-arụ ọrụ ụlọ nyocha, jide aka n'ime okpokolo agba oroma dị n'akụkụ abụọ nke ntọala robot, bulite Flex ahụ n'ala ala, ma tọọ ya n'ala. Chekwaa ma ọ bụ tụfuo ntọala crate na etiti mbupu.

OPENTRONS FLEX

ISI NKE 2: Nwụnye na Mbugharị 11 Iji 12 mm hex L-key sitere na ngwa onye ọrụ, wepụ bolts anọ ahụ na-ejide okpokolo agba mbupu ka
Flex. Chekwaa ma ọ bụ tụfuo fremu na bolts.
12 Wepu aka anọ aluminom na ngwa onye ọrụ. Kpochie aka ndị ahụ n'otu ebe ahụ ejiri bolts mbupu 12 mm.

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU
13 Site n'enyemaka site n'aka onye gị na ya na-arụ ọrụ ụlọ nyocha, welie Flex site na iji aka ya ma bugharịa ya na oche ọrụ maka mgbakọ ikpeazụ.

Nkebi nke 3: Mgbakọ ikpeazụ na ike gbanye
Mgbe ịkwaga Flex na mpaghara ọrụ nwa oge, ma ọ bụ ebe obibi ya na-adịgide adịgide, oge erugo itinye mmecha mmecha na robot ọhụrụ gị.
14 Ọ bụrụ na ibugharịla rọbọt ahụ n'ebe ikpeazụ ya na-arụ ọrụ, wepụ aka na-ebu ma were okpu mmecha dochie ha. Okpu ndị ahụ na-emechi oghere aka na etiti ahụ ma nye robot ahụ ọdịdị dị ọcha. Weghachite aka ndị a na ngwa onye ọrụ maka nchekwa.

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU
15 Weghachite akụkụ elu na akụkụ site na ụfụfụ nkwakọ ngwaahịa nke ị wepụrụ n'elu mgbe ị wepụsịrị akwa akwa ahụ.
16 Kwado akụkụ mpio ahụ na Flex site n'ịgbaso ozi akara n'ihu ihe nkiri nchebe. Mgbe ahụ wepụ ihe nkiri nchebe.
17 N'iji skru windo gbagoro agbagoro na screwdriver 2.5 mm sitere na ngwa onye ọrụ, tinye akụkụ windo na Flex. Jide n'aka na oghere ndị gbara agba (V-shaped) dị n'akụkụ windo na-eche ihu (n'ebe gị). Nke a na-enye ohere ka kposara ahụ dabara na elu nke windo ahụ.

Ịdọ aka ná ntị: Ịhazi akụkụ ahụ n'ụzọ na-ezighi ezi nwere ike ibute mmebi. Oke ịghasa torque nwere ike ịgbawa ogwe. Aka mee ka skru dị ike ruo mgbe akụkụ mpio ahụ nwere nke ọma. Nke a abụghị nnwale nke ike.

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU 18 Iji screwdriver 2.5 mm sitere na ngwa onye ọrụ, wepụ skru mkpọchi na gantry. Ndị a
skru na-egbochi gantry ahụ ịkwagharị mgbe ọ na-agafe. The gantry kpochidoro kposara dị: N'akụkụ aka ekpe okporo ígwè dị nso n'ihu nke robot. N'okpuru ogwe aka gantry kwụ ọtọ. N'akụkụ okporo ụzọ aka nri dị nso n'ihu robot n'ime mgbodo oroma. Enwere skru abụọ ebe a.
Gantry na-eji aka eme ihe ngwa ngwa mgbe ọ wepụsịrị kposara mbupu niile. 19 Bee ma wepụ eriri rọba abụọ ahụ nke na-ejide ebe mkpofu ebe n'oge mbupu.

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU
20 Tinye eriri ọkụ na Flex ma tinye ya na oghere mgbidi. Jide n'aka na mpaghara oche ahụ enweghị ihe mgbochi. Tụgharịa ngbanwe ọkụ n'azụ aka ekpe nke robot. Ozugbo agbanyere ya, gantry na-aga n'ebe obibi ya na ihuenyo mmetụ ahụ na-egosiputa ntuziaka nhazi ndị ọzọ.

Ugbu a Flex gị si na igbe ahụ wee dị njikere ịga, gaa n'ihu na ngalaba Run Run n'okpuru.
2.3 Agba mbụ
Mee ntọala ntọala na ihuenyo mmetụ tupu jikọọ ngwaike ọ bụla ọzọ na Flex gị. Robot ga-eduzi gị site na ijikọ na netwọk ụlọ nyocha gị, imelite na ngwanrọ kachasị ọhụrụ, na ịhazi Flex site n'inye ya aha.
Ike na
Mgbe ị gbanyere Flex, akara Opentron ga-apụta na ihuenyo mmetụ ahụ. Mgbe obere oge gasịrị, ọ ga-egosi ihuenyo "Nnọọ na Opentrons Flex gị".
Ihuenyo nnabata Opentrons Flex. Ị ga-ahụ naanị ihuenyo a mgbe ịmalitere Flex gị maka oge mbụ.

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU
Jikọọ na netwọk ma ọ bụ kọmputa
Soro mkpali dị na ihuenyo mmetụ aka ka ijikọ robot gị ka o wee lelee maka mmelite ngwanrọ wee nweta protocol files. Enwere ụzọ njikọ atọ: Wi-Fi, Ethernet, na USB.

Nhọrọ njikọ netwọkụ. Ịkwesịrị ịnwe njikọ ịntanetị iji melite Flex. Wi-Fi: Jiri ihuenyo mmetụ ahụ jikọọ na netwọk Wi-Fi echekwara na njirimara WPA2 nkeonwe (ọtụtụ netwọk na-achọ naanị paswọọdụ iji sonyere na-ada n'okpuru ụdị a).
Mara: Flex anaghị akwado ọnụ ụzọ ndị eji eji (netwọk na-enweghị paswọọdụ mana na-ebu a webibe iji nyochaa ndị ọrụ mgbe ejikọtara).
Ị nwekwara ike jikọọ na netwọk Wi-Fi mepere emepe, mana akwadoghị nke a.
Ịdọ aka ná ntị: Ijikọ na netwọk Wi-Fi mepere emepe ga-enye onye ọ bụla nọ na nso nso nke mgbama netwọk aka ịchịkwa robot Opentrons Flex gị na-enweghị nyocha.

Ọ bụrụ na ịchọrọ ijikọ na netwọk Wi-Fi nke na-eji nyocha ụlọ ọrụ (gụnyere "eduroam" na netwọk agụmakwụkwọ yiri nke chọrọ aha njirimara na paswọọdụ), buru ụzọ jikọọ na Opentrons App site na Ethernet ma ọ bụ USB iji mechaa nhazi mbụ. Jikọọ na netwọk Wi-Fi ụlọ ọrụ na ntọala netwọk maka Flex gị. Iji nweta ntọala netwọk:

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU
1. Pịa Ngwaọrụ na akụkụ aka ekpe nke Opentrons App. 2. Pịa menu ntụpọ atọ () maka Flex gị wee họrọ Ntọala Robot. 3. Pịa taabụ netwọk.
Họrọ netwọk gị site na menu ndọpụta ma ọ bụ họrọ "Jikọọ netwọk ọzọ..." wee tinye SSID ya. Họrọ usoro nyocha ụlọ ọrụ nke netwọk gị na-eji. Ụzọ ndị akwadoro bụ:
EAP-TTLS nwere TLS EAP-TTLS nwere MS-CHAP v2 EAP-TTLS nwere MD5 EAP-PEAP nwere MS-CHAP v2 EAP-TLS
Ụzọ ọ bụla n'ime usoro ndị a chọrọ aha njirimara na paswọọdụ, yana dabere na nhazi netwọk gị nwere ike ịchọ asambodo files ma ọ bụ nhọrọ ndị ọzọ. Kpọtụrụ akwụkwọ IT nke akụrụngwa gị ma ọ bụ kpọtụrụ onye njikwa IT gị maka nkọwa nke nhazi netwọkụ gị.
Ethernet: Jikọọ robot gị na ngbanwe netwọkụ ma ọ bụ hub na eriri Ethernet. Ị nwekwara ike jikọọ ozugbo na ọdụ ụgbọ mmiri Ethernet na kọmputa gị, malite na ụdị robot 7.1.0.
USB: Jikọọ eriri USB A-na-B enyere na ọdụ ụgbọ mmiri USB-B nke robot na ọdụ ụgbọ mmiri mepere emepe na kọmputa gị. Jiri eriri USB B-na-C ma ọ bụ ihe nkwụnye USB A-na-C ma ọ bụrụ na kọmputa gị enweghị ọdụ ụgbọ mmiri USB-A.
Iji gaa n'ihu na nhazi, kọmpụta ejikọrọ ga-enwerịrị ngwa Opentrons arụnyere ma na-agba ọsọ. Maka nkọwa gbasara ịwụnye ngwa Opentrons, lee ngalaba nwụnye ngwa nke ngwa ngwanrọ na isi ọrụ.
Wụnye mmelite ngwanrọ
Ugbu a ị jikọọla na netwọkụ ma ọ bụ kọmpụta, robot nwere ike lelee mmelite ngwanrọ na ngwa ngwa wee budata ha ma ọ dị mkpa. Ọ bụrụ na enwere mmelite, ọ nwere ike were nkeji ole na ole iji wụnye. Ozugbo emelitere, robot ga-amalitegharị.

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU
Tinye pendant kwụsị ihe mberede
Jikọọ Pendanti Kwụsị Mberede (E-stop) gụnyere n'ọdụ ụgbọ mmiri inyeaka (AUX-1 ma ọ bụ AUX-2) n'azụ robot.

Tupu na mgbe jikọọ Pendanti Kwụsị Mberede.
Ịgbakwunye na ime ka E-stop bụ iwu maka itinye ngwa na usoro ịgba ọsọ na Flex. Maka ozi ndị ọzọ maka iji E-nkwụsị n'oge arụ ọrụ robot, lee ngalaba ihe nkwụsị ihe mberede nke isi nkọwa Sistemu.
Nye robot gị aha
Ịkpọ aha robọt gị na-enye gị ohere ịchọpụta ya ngwa ngwa na gburugburu ụlọ nyocha gị. Ọ bụrụ na ị nwere ọtụtụ roboti Opentrons na netwọk gị, gbaa mbọ nye ha aha pụrụ iche. Ozugbo ị kwadoro aha robot gị, a ga-akpọrọ gị gaa na Opentrons Flex Dashboard gị. Ma eleghị anya, nzọụkwụ ọzọ ị ga-achọ ime bụ itinye ngwá ọrụ, nke a na-ekpuchi na ngalaba na-esote.
2.4 Ntinye akụrụngwa na nhazi
Ka emechara ntọlite robot nke mbụ, nzọụkwụ na-esote bụ itinye ngwa na rọbọt ma mezie ha.
Iji wụnye ngwa, buru ụzọ kpatụ Ngwa na ihuenyo mmetụ aka ma ọ bụ gaa na ngalaba Pipettes na Modules nke ihuenyo nkọwa ngwaọrụ na ngwa Opentrons. Họrọ ugwu efu wee họrọ ma tinye Pipette ma ọ bụ Tinye Gripper. Ọ bụrụ na ejirilarị elu nke ịchọrọ iji, ị ga-ebu ụzọ wepụ pipette ma ọ bụ gripper.

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU
Mara: Usoro nrụnye n'ozuzu bụ otu ihe ahụ n'agbanyeghị ma ị na-eji ihuenyo mmetụ ma ọ bụ ngwa Opentrons. Ngwa ọ bụla ịmalitere ga-ejikwa usoro nrụnye ahụ ruo mgbe ịmechara ma ọ bụ kagbuo ya.
Ọ bụrụ na ibido na ihuenyo mmetụ ahụ, ngwa ahụ ga-egosi robot ka ọ “na-arụ ọrụ”. Ọ bụrụ na ibido na ngwa ahụ, ihuenyo mmetụ ahụ ga-egosi modal na-egosi na ntinye ngwá ọrụ na-aga n'ihu.
Usoro nrụnye kpọmkwem dịgasị iche dabere na ngwá ọrụ ị na-etinye, dị ka ekpuchiri ya na ngalaba n'okpuru. Ngwa niile nwere usoro mmezi akpaaka, nke ị ga-eme ozugbo echichichara.
Ntinye pipette
Mgbe ị wụnye pipette, a ga-eduzi gị site na usoro ndị a na ihuenyo mmetụ ma ọ bụ na Opentrons App.
1. Họrọ ụdị PIPETTE Họrọ n'etiti 1- ma ọ bụ 8-Channel Pipette na 96-Channel Pipette. Ịgbakwunye 96-Channel Pipette chọrọ usoro ọzọ ole na ole n'ihi na ọ na-ejikọta na efere pụrụ iche nke na-agbanye ma ọ bụ pipette.
2. Kwadebe maka ntinye Wepu labware site na oche ma hichaa ebe ọrụ iji mee ka mgbakwunye na nhazi dị mfe. Na-achịkọtakwa akụrụngwa dị mkpa, dị ka nyocha calibration, screwdriver hex, na ịrị elu efere (maka 96-Channel Pipette).
3. Jikọọ na Chekwaa PIPETTE Gantry ga-aga n'ihu nke robot ka i wee nwee ike itinye pipette.
1- na 8-Channel Pipettes jikọọ ozugbo na ugwu pipette. Pipette 96-Channel chọrọ efere nrụnye. Iji tinye efere na-arị elu, ị ga-ebu ụzọ wepụ ụgbọ ala z-axis maka ugwu pipette ziri ezi.

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU
Jikọọ pipette na ugwu pipette a họọrọ ma chekwaa skru ya.
4. Na-agba ọsọ CALIBRATION AUTOMATED Iji mee ka pipette dị nro, tinye ihe nyocha nhazi na oghere pipette kwesịrị ekwesị. pipette ga-emegharị ozugbo iji metụ isi ihe ụfọdụ dị na oche ma chekwaa ụkpụrụ nhazi ndị a maka ojiji n'ọdịnihu. Ozugbo mmezigharị agwụla ma wepụrụ nyocha ahụ, pipette ga-adị njikere maka ojiji na usoro iwu.
Ntinye Gripper
Mgbe ị wụnye gripper, a ga-eduzi gị site na usoro ndị a na ihuenyo mmetụ ma ọ bụ na ngwa Opentrons.
1. Kwadebe maka ntinye Wepu labware site na oche ma hichaa ebe ọrụ iji mee ka mgbakwunye na nhazi dị mfe. Chịkọtakwa screwdriver hex chọrọ ma hụ na ntụtụ calibration dị na ebe nchekwa ya na gripper.
2. Jikọọ na Chekwaa GRIPPER Gantry ga-aga n'ihu nke robot ka ị nwee ike itinye gripper. Jikọọ gripper na mgbati ndọtị ma chekwaa skru ya.
3. Gbaa CALIBRATION akpaghị aka Ka ịdozi ihe njide, tinye ntụtụ nhazi n'ihu n'agba. The gripper ga-akpaghị aka na-akpali emetụ aka ụfọdụ isi ihe na oche na-echekwa ndị a calibration ụkpụrụ maka ọdịnihu ojiji. Mgbe ahụ megharịa otu usoro ahụ site na ntụtụ nhazi n'azụ agba. Ozugbo mmezigharị agwụla ma tinyeghachi ntụtụ ahụ na ebe nchekwa ya, gripper ga-adị njikere maka ojiji na ụkpụrụ.
2.5 Mbugharị
Akụkụ a na-enye ndụmọdụ na ntụziaka gbasara otu esi ebugharị rọbọt Opentrons Flex gị n'ofe dị mkpụmkpụ na ogologo.

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU
Ntugharị dị mkpirikpi
Ntugharị dị mkpirikpi na-adị anya site na "ka anyị bugharịa ya ntakịrị" gaa n'ofe ụlọ nyocha, n'okpuru ụlọ nzukọ, ma ọ bụ ala ọzọ n'ime ụlọ gị. N'ọnọdụ ndị a, ị nwere ike ibugharị Flex gị n'aka. Ibufe ya na ụgbọ ala aka bụkwa nhọrọ dị mma.
Ịdọ aka ná ntị: Flex dị kilogram 88.5. N'ihi ya, ọ na-achọ mmadụ abụọ ka ha bulie ma bugharịa ya n'enweghị nsogbu.
Tinyegharịa aka elu ka ibugharịa Flex gị gaa na ebe ọhụrụ dị nso. Ibulite na iburu Flex site na aka ya bụ ụzọ ziri ezi isi bugharịa robot dị anya. Wepu aka ma chekwaa ha na ngwa onye ọrụ ka emechara mmegharị ahụ. Iji gbochie imebi robọt ahụ, na-eji aka ebuli elu bulie ya ma bugharịa ya. Ejidela etiti ka ebuli ma ọ bụ bugharịa roboti gị.
Ntugharị dị anya
Ntugharị dị anya na-ebuga Flex gị na mgbanaka nke mahadum, akụrụngwa, ma ọ bụ ụlọ ọrụ gị. N'ofe obodo, gaa n'obodo ọhụrụ, steeti, mpaghara, ma ọ bụ obodo niile bụ examples nke njem dị anya. N'okwu a, ị ga-achọ ịkwakọ Flex iji chebe ya pụọ na mmetụ, ihe mgbagwoju anya na mmegharị siri ike nke nwere ike ime mgbe ị na-agafe.
Ọ bụrụ na idowela igbe mbupu na nkwado ime nke jiri Flex gị bịa, ị nwere ike tinyeghachi ya na ngwa ndị a maka njem dị anya. Soro usoro iwepụ igbe n'azụ ka iji kwado Flex gị maka njem dị anya. N'ezie, ị kwesịrị:
Kwupu ike na eriri netwọk, ọ bụrụ na etinyere ya. Wepu ngwaike niile agbakwunyere na labware. Tinyegharịa efere oche. Mkpọchi gantry (lee ngalaba ndụmọdụ ịkwaga izugbe n'okpuru). Wepu ma chekwaa akụkụ windo.
Ọ bụrụ na ị debere akpa akpa ahụ:
Tinyegharịa etiti mbupu na Flex wee chekwaa ya na pallet base site na iji L-brackets. Tinye padding ma chịkọtakwa igbe mbupu.

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU
Ọ bụrụ na ịnweghị akpa akpa na ihe metụtara, kpọtụrụ ụlọ ọrụ mbupu ama ama. Ha nwere ike ijikwa nkwakọ ngwaahịa, ụgbọ njem na usoro nnyefe maka gị.
Ndụmọdụ na-akpali akpali n'ozuzu
Kwụpụ ike na eriri netwọk Tupu ịkwaga Flex gị, echefula ime: Gbanyụọ ọkụ wee wepụ ya na ọkụ ọkụ. Kwupu eriri Ethernet ma ọ bụ eriri USB, ọ bụrụ na ejiri ya.
Kpọchie ndị agha ahụ Tupu ịkwaga Flex gị, tinyeghachi skru mkpọchi iji jide gantry ahụ. Ebe mkpọchi gantry dị: N'akụkụ okporo ụzọ aka ekpe dị nso n'ihu robot. N'okpuru ogwe aka gantry kwụ ọtọ. N'akụkụ okporo ụzọ aka nri dị nso n'ihu robot. Ikpuchi akụkụ a nke gantry chọrọ obere
ihe nkwado oroma na skru abụọ akpọchi.

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU
ỤLỌ NKE GANTRY Ị nwere ike ị gaghị achọ igbachi gantry ma ọ bụrụ na ị na-ebuga rọbọt n'ebe dị nso. Ọ bụrụ na ị kpebie na ị gaghị akpọchi ya, opekata mpe, jiri ihu mmetụ ahụ ma ọ bụ ngwa Opentrons ziga gantry n'ọkwa ụlọ ya tupu ịgbanye ya ike. Iji banye n'ụlọ site na ihuenyo mmetụ aka, kpatụ menu ntụpọ atọ () wee pịa ụlọ gantry. Iji nweta ụlọ ahụ site na ngwa Opentrons: Pịa Ngwaọrụ. Pịa na Flex gị na listi ngwaọrụ. Pịa menu ntụpọ atọ () wee pịa Home gantry.
Wepu modul dị n'ime ụlọ yana mgbakwunye ndị ọzọ na-agbakwunye ibu na Flex gị. Ha na-emetụtakwa etiti rọbọt nke ike ndọda, nke nwere ike ime ka ọ na-adị ya ka ọ “na-afụ ụfụ” mgbe ọ na-ebuli ya. Iji nyere aka mee ka rọbọt dị mfe ma dozie ya, wepụ ngwa ọ bụla jikọtara ya na labware tupu ị bulie ya.
KWESỊRỊ DECK SLOTS Anyị na-akwado ịgbakwunye oghere oche maka njem dị anya. Ịchekwa oghere ndị ahụ na ebe mbụ ha na-enyere aka igbochi mfu mberede. Ịgbakwunye oghere oche maka obere mmegharị gburugburu ụlọ nyocha bụ nhọrọ.
Post-ịkwagharị replalibration ị kwesịrị ịza ngwa ọ bụla na modulu mgbe ị gụghachiri ha. Maka nkọwa ndị ọzọ gbasara nhazi modul, lee isi nke Modul.

OPENTRONS FLEX

ISI NKE 2: NKWUKWU NA NKWUKWU
Echiche ikpeazụ banyere ịkwaga
Flex gị bụ igwe siri ike ma rụkwaa nke ọma, mana ọ bụkwa ngwa sayensị nke emebere iji nweta nnabata. N'ihi ya, ị kwesịrị iji nlezianya na-emeso ya mgbe ị na-ebuga ya na mpaghara ọrụ mpaghara gị ma ọ bụ na-eziga ya na mba ahụ. Nke a pụtara ịgbaso ntuziaka enyere ebe a na iji amamihe nke onwe gị gbasara otu esi ebufe ngwa ụlọ nyocha dị oke ọnụ. N'okpuru ala: mgbe ị na-akpụgharị Flex gị, hie ụzọ n'akụkụ ịkpachara anya na mgbakwunye mgbakwunye.
Ọ bụrụ na ị nwere ajụjụ ma ọ bụ nchegbu gbasara mbugharị Flex gị, kpọtụrụ anyị na support@opentrons.com.

OPENTRONS FLEX

ISI NKE 3
Nkọwa sistemu

Isiakwụkwọ a na-akọwa sistemụ ngwaike nke Opentrons Flex, nke na-adabere na njirimara akpaaka ụlọ nyocha ya. Ụlọ elu, gantry, na ngwá ọrụ nke Opentrons Flex na-enyere aka iji nkenke mmiri mmiri na ihe ejizi labware. Ihuenyo mmetụ ngwaọrụ na-enyere aka ịgba ọsọ protocol na ịlele ọkwa robot na-enweghị mkpa ibubata kọmputa gị na bench ụlọ nyocha. Njikọta ikuku na ikuku na-enyere njikwa ndị ọzọ sitere na ngwa Opentrons (lee akụkụ Software na arụmọrụ maka nkọwa ndị ọzọ) yana ịgbatị atụmatụ sistemụ site na ijikọta akụkụ (lee isiakwụkwọ modulu).

3.1 Akụkụ anụ ahụ

Igwefoto

Ìhè ọnọdụ

Ihuenyo mmetụ aka

Frame

Ụgbọ mmiri Gantry
Ọnụ ụzọ ihu
Ebe akụkụ anụ ahụ nke Opentrons Flex.

Akụkụ Windows Handle Caps

OPENTRONS FLEX

ISI NKE 3: Nkọwa usoro

Frame na mkpuchi
Okpokoro nke Opentrons Flex robot na-enye nkwado siri ike na nhazi maka oche ya na gantry ya. Sistemụ sistemu arụ ọrụ niile dị na ma tinye ya na etiti isi. A na-eji ígwè mpempe akwụkwọ na aluminom extrusions rụọ etiti ahụ.
Igwe igwe ahụ nwere oghere maka windo n'akụkụ yana ọnụ ụzọ dị n'ihu nke polycarbonate pụtara nke na-eme ka ị hụ ihe na-eme n'ime Flex. Ọnụ ụzọ n'ihu na-emeghe maka ịbanye n'ime ime nke usoro ahụ. Site n'ọnụ ụzọ ọnụ ụzọ meghere, ị nwere ike itinye ngwa, modul, na ihe ndozi oche; kwadebe oche tupu a protocol; ma ọ bụ megharịa ọnọdụ nke oche n'oge a protocol.
Iberibe ọkụ ọkụ na-acha ọcha n'ime akụkụ elu nke etiti ahụ na-enye ọkụ ambient na-achịkwa ngwanrọ. Igwefoto 2-megapixel nwere ike ịse foto oche na ebe ọrụ maka ịdekọ na nsuso usoro ogbugbu.

Dek na ngalaba ọrụ
Ogbe ahụ bụ elu aluminom arụpụtara nke a na-eme ụkpụrụ sayensị akpaghị aka na ya. Ogbe ahụ nwere oghere ANSI/SLAS isi iri na abụọ nke enwere ike ịhazigharị iji jide labware, modul, na ihe oriri. A na-amata oghere oche ahụ site na usoro nhazi, yana oghere A12 n'azụ aka ekpe yana oghere D1 n'ihu aka nri.

Oghere Mgbasawanye (maka Thermocycler) Mpaghara Ọrụ

StagMpaghara

Mpaghara nke oche n'ime Flex.

OPENTRONS FLEX

ISI NKE 3: Nkọwa usoro
Ebe a na-arụ ọrụ bụ oghere anụ ahụ dị n'elu oche nke nwere ike ịnweta pipetting. Labware etinyere na oghere A1 ruo D3 dị na mpaghara ọrụ.
Opentrons Flex na-abịa na oghere oche mbughari maka ọnọdụ iri na abụọ na mpaghara ọrụ. Oghere oche nke ọ bụla nwere mkpachị nkuku labware maka idowe labware n'elu oche ahụ.
Ị nwere ike ịhazigharị oche ahụ site na iji ihe ndozi oche ndị ọzọ dochie oghere, gụnyere ihe mkpofu a na-ebugharị, ihe mkpofu na modul caddies. A na-eji oghere mgbasawanye n'azụ A1 mee ka ọnụ ụlọ ọzọ maka Thermocycler Module, nke nwere oghere A1 na B1.
Mara: Oghere oche na-agbanwe n'ime kọlụm (1, 2, ma ọ bụ 3) mana ọ bụghị n'ofe ogidi; oghere 1 na kọlụm 3 bụ iberibe dị iche iche n'agbanyeghị nha ha yiri ya. Ị nwere ike ịmata kọlụm nke oghere na-abanye site n'ịtụgharị obere ihe nleba anya na-acha anụnụ anụnụ n'azụ aka ekpe.
Ịkwesịrị ịhapụ oghere oche arụnyere na ebe ịchọrọ idowe labware kwụ ọtọ. Ogbe ahụ na ihe ndị edobere na ya na-anọgide na-agbanwe agbanwe, ọ gwụla ma gripper ma ọ bụ aka aka kpaliri.
Stagtinye ebe
Ndị stagMpaghara ing bụ ohere ọzọ n'akụkụ aka nri nke oche ahụ. Ị nwere ike ịchekwa labware na ebe a mgbe ị wụnyechara stagoghere mpaghara oghere. Labware etinyere na oghere A4 ruo D4 dị na stagmpaghara. Flex pipettes enweghị ike ịbanye na stagmpaghara, mana gripper nwere ike bulie ma bugharịa labware gaa na site na ebe a. Ịtinye oghere ndị ọzọ na-enyere aka idobe ebe ọrụ maka akụrụngwa eji na protocol akpaghị aka gị.
StagA na-etinye oghere mpaghara n'ụfọdụ nhazi ọrụ ma dịkwa maka ịzụta site na https://shop.opentrons.com.

OPENTRONS FLEX

ISI NKE 3: Nkọwa usoro

StagMpaghara nwere oghere arụnyere

Ihe ndozi oche
Ndozi bụ ngwaike nke na-edochi oghere oche ọkọlọtọ. Ha na-ahapụ gị ka ị hazie nhazi oche ma tinye ọrụ na Flex gị. Ugbu a, ihe ndozi oche gụnyere stagOghere ebe a na-adọba ụgbọala, ebe a na-ekpofu ahịhịa dị n'ime, na ebe mkpofu mpụta. Ị nwere ike ịwụnye ngwa nrụnye na oghere oche ole na ole akọwapụtara. Tebụl na-esonụ na-edepụta ebe oche maka ihe ọ bụla.

Fixture Stagoghere mpaghara ahịhịa ahịhịa ahịhịa ahịhịa nwere stagoghere oghere

Oghere oghere A3D3 A1D1 na A3-D3 D3 naanị D3

Ihe nrụnye enweghị ike. Ha enweghị akụrụngwa eletrọnịkị ma ọ bụ igwe arụrụ arụ nke na-agwa robọt ọnọdụ ọnọdụ ha ugbu a na ebe oche ha nọ. Nke a pụtara na ị ga-eji atụmatụ nhazi oche ahụ mee ka Flex mara ihe agbakwunyere na oche na ebe ha dị.

OPENTRONS FLEX

ISI NKE 3: Nkọwa usoro

Ị nwere ike ịnweta ntọala nhazi oche site na ihuenyo mmetụ site na menu ntụpọ atọ () yana site na ngwa Opentrons. Hụ ngalaba nhazi nke sọftụwia na arụmọrụ maka ozi gbasara otu esi ahazi oche ahụ site na ihuenyo mmetụ ahụ.

Ụlọ mkpofu
Opentrons Flex Waste Chute na-ebufe mmiri mmiri, ntụmadị, ọnụ ọnụ ọnụ, na efere ọma site na ogige Flex gaa na ebe mkpofu ahịhịa edobere n'okpuru oghere mpụta ya. Ụlọ ihe mkpofu na-ejikọta na nkwụnye efere oche nke dabara na oghere D3. Ọ na-abịa na oghere ọkara pụrụ iche nke na-eme ka chute ahụ gbasaa n'ihu nke robot.
Akụkụ nke mkpofu ahịhịa.

Ihe nkwụnye efere mkpuchi mkpuchi

Ụlọ mkpofu
Deck Plate Adapter nwere StagMpaghara

Stagoghere mpaghara oghere
StagOghere mpaghara bụ ANSI/SLAS oche dakọtara na-anọchi oghere ọkọlọtọ na kọlụm 3 wee tinye oghere ọhụrụ na s.tagmpaghara mpaghara - niile na-enweghị ohere na mpaghara ọrụ. Ị nwere ike ịwụnye otu oghere ma ọ bụ karịa oghere anọ iji mepụta kọlụm ọhụrụ (A4 ruo D4) n'akụkụ aka nri nke oche ahụ. Mara, Otú ọ dị, na dochie oghere oche A3 chọrọ ịkwaga ebe mkpofu. Site n'ịgbakwunye stagN'ime oghere mpaghara n'elu oche, robot Flex gị nwere ike ịchekwa ọtụtụ labware ma rụọ ọrụ nke ọma.

OPENTRONS FLEX

Flex stagoghere oghere.
49

ISI NKE 3: Nkọwa usoro
Nwụnye oghere
Iji wụnye, wepụ skru na-etinye oghere ọkọlọtọ na oche ma jiri s dochie yatagoghere oghere. Mgbe echichi gasịrị, jiri ihuenyo mmetụ aka ma ọ bụ Opentrons App gwa robot ị gbakwunyere dị katagoghere oghere na oche.
Ịwụnye dị katagoghere oghere.

NKWUKWU SLOT Stagoghere mpaghara dakọtara na ngwa Flex, modul, na labware edepụtara n'okpuru.

Akụkụ Flex Gripper Pipettes Modul
Labware

Stagndakọrịta mpaghara
Flex Gripper nwere ike ibugharị labware gaa na ma ọ bụ site na stagoghere oghere.
Flex pipettes enweghị ike iru stagmpaghara. Jiri gripper bugharịa racks na labware site na staging ebe na-arụ ọrụ ebe tupu pipetting.
Enwere ike itinye Magnetic Block GEN1 na kọlụm 3 n'elu dị katagoghere oghere. Anaghị akwado modul na kọlụm 4.
Modul kwadoro dị ka Heater-Shaker na Temperature Module dabara n'ime caddies nke nwere ike itinye na kọlụm 3. Ị nweghị ike ịgbakwunye dị kataging ebe oghere gaa n'ọkwa nke modul caddy ji.
StagOghere mpaghara nwere otu ANSI/SLAS dị ka oghere oche ọkọlọtọ. Jiri labware dakọtara na gripper na stagmpaghara ing, ma ọ bụ jiri aka tinye ma wepụ labware n'ebe a.

OPENTRONS FLEX

ISI NKE 3: Nkọwa usoro
Usoro mmegharị
Agbakwunyere na etiti ahụ bụ gantry, nke bụ usoro ngagharị na nhazi nke robot. Gantry ahụ na-aga iche n'akụkụ x- na y-axis iji dobe pipettes na gripper n'ebe ziri ezi maka mmezu ụkpụrụ. Ntugharị n'akụkụ anyụike ndị a bụ kpọmkwem na 0.1 mm kacha nso. A na-achịkwa gantry ahụ site na moto 36 VDC ngwakọ bipolar stepper. N'aka nke ya, a na-ejikọta ya na gantry bụ ọkpọkọ pipette na mgbatị ahụ. Ndị a na-aga n'akụkụ z-axis iji dobe pipettes na gripper n'ebe kwesịrị ekwesị maka ogbugbu protocol. A na-ejikwa moto 36 VDC ngwakọ bipolar stepper na-achịkwa mmegharị n'akụkụ axis a. Ngwa elektrọnik dị na gantry na-enye 36 VDC ike na nkwukọrịta na pipettes na gripper, mgbe etinyere ya.
Gantry

Pipette Ugwu

Ugwu ndọtị

Ebe a na-agbanye ngwá ọrụ na Flex.

Ihuenyo mmetụ na ihe ngosi LED
Ihe nrụnye onye ọrụ bụ isi bụ ihuenyo mmetụ LCD 7 inch, nke dị n'ihu aka nri nke robot. A na-ekpuchi ihuenyo mmetụ ahụ na Gorilla Glass 3 maka ọkọ na nguzogide mmebi. Nweta ọtụtụ atụmatụ Flex ozugbo na ihuenyo mmetụ, gụnyere:

OPENTRONS FLEX

ISI NKE 3: Nkọwa usoro

Nhazi usoro njikwa protocol, mkpochapụ, na nlekota njikwa Labware Ntọala Robot Ngwanrọ sistemu na mmelite firmware Ndekọ ọrụ yana ọkwa njehie.
Maka ozi ndị ọzọ maka iji Flex site na ihuenyo mmetụ ahụ, hụ ngalaba ọrụ Touchscreen nke akụkụ Software na Ọrụ.
Ọkụ ọkwa bụ eriri LED dị n'akụkụ elu nke robot nke na-enye ozi nleba anya gbasara robot. Agba dị iche iche na ụkpụrụ nke ọkụ nwere ike ịkọwapụta ihe ịga nke ọma dị iche iche, ọdịda, ma ọ bụ okwu efu:

Ikanam agba White nọpụ iche
Green Normal steeti
Blue amanyere iwu na-ekwu na steeti na-adịghị mma na-acha odo odo steeti Emergency Red Emergency

Ikanam ụkpụrụ Solid Pulsing
Na-enwu gbaa ugboro abụọ
Ọkpụkpụ Pulsing siri ike

Ọkwa robot
Gbanyere na anaghị arụ ọrụ usoro Robot nọ n'ọrụ (dịka, imelite sọftụwia ma ọ bụ firmware, ịtọlite protocol run, ịkagbu ịgba ọsọ protocol) Ọrụ ezuola (dịka ọmụmaatụ, echekwara protocol, emelitere sọftụwia, ngwa mgbakwunye ma ọ bụ kewapụrụ) Protocol na-arụ ọrụ Protocol zuru ezu. Akwụsịla protocol

siri ike

Njehie ngwanrọ

Na-atụgharị anya ugboro atọ, ugboro ugboro

Njehie anụ ahụ (dịka ọmụmaatụ, okuku ngwa)

Enwere ike gbanyụọ ọkụ ọkwa na ntọala robot.

OPENTRONS FLEX

ISI NKE 3: Nkọwa usoro
3.2 Pipettes
Opentrons pipettes bụ ngwaọrụ ahazi nke a na-eji na-ebugharị mmiri mmiri n'akụkụ ebe a na-arụ ọrụ n'oge a na-eme usoro iwu. Enwere ọtụtụ Opentrons Flex pipettes, nke nwere ike ijikwa mpịakọta sitere na 1 µL ruo 1000 µL na ọwa 1, 8, ma ọ bụ 96:
Opentrons Flex 1-Channel Pipette (1µL) Opentrons Flex 50-Channel Pipette (1 µL) Opentrons Flex 5-Channel Pipette (1000 µL) Opentrons Flex 8-Channel Pipette (1) µL)
Pipettes na-ejikọta na gantry na-eji kposara n'agha n'ihu pipette. 1-ọwa na 8-ọwa pipettes nke ọ bụla na-eburu otu pipette ugwu (aka ekpe ma ọ bụ aka nri); pipette nke ọwa 96 na-eburu n'elu abụọ ahụ. Maka nkọwa gbasara ịwụnye pipettes, lee Nrụnye Ngwa na Calibration.

Mgbakwụnye n'agha
kposara

Mgbakwụnye mgbakwụnye n'agha

Onye na-achụpụ

Nozzles (o-mgbanaka enwere ike dochie ya)

Nozzles (O-mgbanaka edobere)

Ebe akụkụ nke 1-, 8-, na 96-ọwa pipettes.

Onye na-achụpụ

OPENTRONS FLEX

ISI NKE 3: Nkọwa usoro

Pipettes na-eburu ndụmọdụ rọba nke a na-atụfu n'efu site na ịpị ha n'elu pipette nozzles, wee jiri ndụmọdụ ndị ahụ na-achọ ma na-ekesa mmiri mmiri. Ọnụ ọgụgụ ike nke achọrọ maka mbuli na-abawanye ka a na-ebulite ndụmọdụ ndị ọzọ n'otu oge. Maka ọnụ ọgụgụ dị nta nke ndụmọdụ, pipette na-ejikọta ndụmọdụ site n'ịkwanye nozzle pipette ọ bụla n'ime ọnụ. Iji nweta ike dị mkpa iji bulie ndụmọdụ zuru oke, pipette nke ọwa 96 na-adọtakwa ndụmọdụ ahụ elu na nozzles. Ihe ndọkpụ a na-achọ ka ịtinye racks n'ime ihe nkwụnye ọkụ n'ọnụ, karịa ozugbo na oghere oche. Iji tụfuo ndụmọdụ (ma ọ bụ weghachi ha n'akpa ha), usoro pipette ejector na-akwapụ ntụmadị nke nozzles.

Nkọwapụta pipette
Emebere Opentrons Flex pipettes ka ọ na-ejikwa ọtụtụ mpịakọta. N'ihi oke ha zuru oke, ha nwere ike iji ọtụtụ ndụmọdụ dị iche iche, nke na-emetụta njirimara njikwa mmiri mmiri ha. Opentrons anwalela Flex pipettes maka izi ezi na nkenke n'ọtụtụ ọnụ na ngwakọta olu mmiri mmiri:

Pipette
Flex 1-Ọwa
50 µL
Flex 1-Ọwa
1000 µL
Flex 8-Ọwa
50 µL
Flex 8-Ọwa
1000 µL

Ntụpọ ike 50 µL 50 µL 50 µL 50 µL 50 µL 200 µL 1000 µL 50 µL

Nwalere olu 1 µL 10 µL 50 µL 5 µL 50 µL
200 µL 1000 µL
1 µL 10 µL 50 µL 5 µL 50 µL 200 µL 1000 µL

Izi ezi %D 8.00% 1.50% 1.25% 5.00% 0.50% 0.50% 0.50% 10.00% 2.50% 1.25% 8.00% 2.50% 1.00% 0.70%

Nkenke %CV 7.00% 0.50% 0.40% 2.50% 0.30% 0.15% 0.15% 8.00% 1.00% 0.60% 4.00% 0.60% 0.25% 0.15%

OPENTRONS FLEX

ISI NKE 3: Nkọwa usoro

Flex 96-Ọwa
1000 µL

50 µL 50 µL 200 µL 1000 µL

5 µL 50 µL 200 µL 1000 µL

10.00% 2.50% 1.50% 1.50%

5.00% 1.25% 1.25% 1.50%

Buru ozi ziri ezi a n'uche mgbe ị na-ahọrọ ndụmọdụ maka pipette gị. N'ozuzu, maka nsonaazụ kacha mma ị ga-eji ndụmọdụ kacha nta na-egbo mkpa nke protocol gị.

Mara: Opentrons na-eme nnwale volumetric nke Flex pipettes iji hụ na ha zutere izi ezi na nkọwapụta nke edepụtara n'elu. Ịkwesighi ịhazigharị ụda nke pipettes gị na-enye tupu eji ya. Naanị ị ga-eme mmezi ọnọdụ. Hụ ngalaba na-esote, yana ngalaba nrụnye Pipette nke isi nrụnye na mbugharị, maka nkọwa.
Ọrụ Opentrons Care na Opentrons Care Plus gụnyere nnọchi pipette kwa afọ yana asambodo nhazi. Hụ ngalaba ọrụ Flex nke Mmezi na Isi Ọrụ maka nkọwa.

Nhazi pipette
Ngwa onye ọrụ na-agụnye nyocha ngbanwe igwe pipette, nke ị na-eji n'oge nhazi ọkwa. Mgbe a na-agba ọsọ protocol, chekwaa nyocha ahụ n'enweghị ihe na-ejide magnet n'ihu ogidi nke robot. N'oge nhazi nhazi, tinye nyocha ahụ na nozzle kwesịrị ekwesị ma kpochie ya. Robot ahụ na-ebuga nyocha ahụ gaa n'ebe nrụzigharị n'elu oche ahụ iji tụọ kpọmkwem ọnọdụ pipette ahụ.
Ihe nkwụnye ọkụ n'ọnụ Pipette
Ụgbọ mmiri pipette nke Opentrons Flex 96 nwere ihe nkwụnye ọnụ anọ. Ndị a bụ brackets aluminom kpụrụ akpụ nke ị na-etinye n'elu oche. Ihe nkwụnye ọkụ na-ejide Flex 50L, 200L na 1000µL racks.

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ISI NKE 3: Nkọwa usoro
N'ihi ike dị na ya, pipette nke ọwa 96 chọrọ ihe nkwụnye iji tinye ọnụ n'ọnụ zuru ezu nke ọma. N'oge usoro ntinye aka, pipette na-agagharị n'elu nkwụnye ahụ, na-agbada onwe ya na ntụtụ na-arị elu, ma na-adọta ndụmọdụ na pipettes site na ibuli ihe nkwụnye ọkụ na nkwụnye ọnụ. Ịdọrọ ndụmọdụ ahụ, kama ịkwanye, na-enye ike dị mkpa iji chekwaa ndụmọdụ na pipettes ma gbochie ịgbagha elu oche ahụ. Mgbe emechara ya, pipette nke ọwa 96 na-ewetu ihe nkwụnye ọkụ na sọks efu n'elu oche ahụ. Hụ ngalaba ndụmọdụ na ndụmọdụ nke isi Labware maka ozi ndị ọzọ.
mbuli n'ọnụ akụkụ
Pipette nke ọwa 96 nwere ike iburu ndụmọdụ zuru oke ma ọ bụ ọnụ ọgụgụ dị nta nke ndụmọdụ. Nke a na-abawanye ọnụ ọgụgụ nke ngwa ị nwere ike iji pipette 96-channel rụọ ọrụ, ebe ọ bụ na ọ na-ejide ma ọ bụ pipette mounts.
Ugbu a, pipette nke ọwa 96 na-akwado ntinye aka n'akụkụ maka ndụmọdụ 8 na nhazi kọlụm. N'ime nhazi a, pipette na-eji nozzles aka ekpe ya na-eburu ndụmọdụ aka nri ruo n'aka ekpe site na ngwungwu ọnụ ọnụ, ma ọ bụ nozzles aka nri ya iji bulie ndụmọdụ aka ekpe gaa n'aka nri site n'ọnụ ọnụ.
Mgbe ị na-ebuli ihe na-erughị ndụmọdụ 96 site n'ọnụ ọnụ ọnụ, a ga-edobe rack ozugbo na oche, ọ bụghị na nkwụnye nkwụnye n'ọnụ.
Ihe mmetụta Pipette
Opentrons Flex pipettes nwere ọtụtụ sensọ na-achọpụta ma dekọọ data gbasara ọnọdụ pipette na ndụmọdụ ọ bụla ọ welitere.
Ihe mmetụta mmetụta
Na mgbakwunye na nyocha igwe ma ọ bụ ihe na-eduzi, ihe mmetụta ike na-achọpụta mgbe pipette na-emekọ ihe. Nchọpụta nke kọntaktị n'etiti metal nyocha na oche na-eji na akpaghị aka pipette calibration na modul nhazi usoro.
1-ọwa pipettes nwere otu ihe mmetụta capacitance, ebe multi-channel pipettes nwere abụọ: na ọwa 1 na 8 nke 8-ọwa pipettes, na na ọwa 1 na 96 (ọnọdụ A1 na H12) nke 96-channel pipette.

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ISI NKE 3: Nkọwa usoro

NDỤMỌDỤ anya anya ọnụnọ ihe mmetụta
Mgbanwe fotointerruptor na-achọpụta ọnọdụ nke pipette's n'ọnụ ejector usoro, na-akwado ma ọ bụrụ na ewelitere ma ọ bụ tufuo ndụmọdụ nke ọma. 1-ọwa, 8-ọwa, na 96-ọwa pipettes niile nwere otu ihe mmetụta anya nke na-enyocha ntinye aka n'ofe ọwa niile.
Pipette firmware mmelite
Opentrons Flex na-emelite firmware pipette na-akpaghị aka iji dobe ya na ụdị ngwanrọ robot. Mmelite ngwa ngwa Pipette na-adịkarị ngwa ngwa, na-eme mgbe ọ bụla:
Ị na-etinye pipette. Robot malitegharịa.
Ọ bụrụ na, n'ihi ihe ọ bụla, pipette firmware gị na ụdị ngwanrọ robot pụọ na mmekọrịta, ị nwere ike iji aka melite ngwa ngwa na ngwa Opentrons.
1. Pịa Ngwaọrụ. 2. Pịa na Flex gị na listi ngwaọrụ. 3. N'okpuru akụrụngwa na modul, pipette na-apụ apụ ga-egosi ịgụ akwụkwọ ọkọlọtọ ịdọ aka ná ntị
"Nwelite firmware dị." Pịa Mmelite ugbu a ka ịmalite mmelite.
Ị nwere ike view ụdị firmware arụnyere ugbu a nke pipette ọ bụla agbakwunyere. Na ihuenyo mmetụ ahụ, gaa na Ngwa wee pịa aha pipette. Na ngwa Opentrons, chọta kaadị pipette n'okpuru Ngwa na Modules, pịa menu ntụpọ atọ (), wee pịa Banyere pipette.

3.3 Gripper
The gripper na-ebugharị labware n'akụkụ ebe ọrụ yana staging ebe n'oge ogbugbu nke protocol. Onye na-ejide ihe na-ejikọta ya na mgbatị mgbatị ahụ, nke dị iche na n'elu pipette; Enwere ike iji gripper na nhazi pipette ọ bụla. Maka nkọwa gbasara ịwụnye gripper, lee Nwụnye Ngwa na Calibration.
Ihe gripper nwere ike ibugharị labware n'ofe oche ahụ wee banye ma ọ bụ gbanyụọ nke modul. Ihe njide ahụ nwere ike ijikwa ụfọdụ efere olulu mmiri nwere uwe mwụda zuru oke, efere olulu mmiri miri emi, na akwa ntụ. Maka nkọwa ndị ọzọ gbasara ihe labware onye gripper nwere ike ịkwaga, hụ ngalaba Labware na Opentrons Flex Gripper nke isi Labware, ma ọ bụ gaa na Ọbá akwụkwọ Opentrons Labware.

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ISI NKE 3: Nkọwa usoro

Nkọwapụta Gripper
Anụ ahụ na-arụ ọrụ bụ isi nke gripper, nke bụ imeghe ma ọ bụ mechie paddle abụọ yiri ya iji tinye ma ọ bụ hapụ ike n'akụkụ nke labware. A na-ejikwa moto brushed 36 VDC jikọtara na sistemu gia rack na pinion.
Iji bugharịa otu mpempe labware nke agba agbajidere, gantry na-ebuli gripper n'akụkụ z-axis, na-ebugharị ya n'akụkụ, wee wedata ya n'ọnọdụ ọhụrụ labware.
Ebe akụkụ nke gripper.

Mgbakwụnye skru
Ndozi ntụtụ Jaws Paddles

Nhazi nke Gripper
Ihe njide na-agụnye ntụtụ nhazigharị igwe. Ntụtụ nhazi ahụ dị n'ime ebe nchekwa echekwabara na akụkụ ala nke gripper. Ihe ndọta na-ejide ntụtụ ahụ n'ebe. Iji wepu ntụtụ nhazi, jiri mkpịsị aka gị jide ya wee dọpụta nwayọ. Iji dochie ntụtụ ahụ, tinyeghachi ya na oghere nchekwa. Ị ga-ama na ọ dị nchebe mgbe ọ batara n'ebe.
Mgbe ị na-edozi gripper, tinye ntụtụ ahụ n'agba nke ọ bụla n'aka nke ya. Robọt ahụ na-ebuga ntụtụ ahụ gaa n'ihe nrụzigharị n'elu oche iji tụọ kpọmkwem ọnọdụ gripper ahụ.
Mgbe protocol na-agba ọsọ, tinye pin na ebe nchekwa ya maka nchekwa. Kpọtụrụ anyị na support@opentrons.com ma ọ bụrụ ntụtụ calibration efunahụ gị.

Nwelite ngwa ngwa Gripper
Opentrons Flex na-emelite ngwa ngwa gripper na-akpaghị aka iji dobe ya na ụdị ngwanrọ robot. Mmelite ngwa ngwa Gripper na-adịkarị ngwa ngwa, na-eme mgbe ọ bụla:

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ISI NKE 3: Nkọwa usoro
Ị na-etinye gripper. Robot malitegharịa.
Ọ bụrụ na, n'ihi ihe ọ bụla, gripper firmware gị na ụdị sọftụwia robot pụọ na mmekọrịta, ị nwere ike iji aka kwalite ngwa ngwa na ngwa Opentrons.
1. Pịa Ngwaọrụ. 2. Pịa na Flex gị na listi ngwaọrụ. 3. N'okpuru akụrụngwa na modul, onye na-apụ apụ na-emekọrịta ihe ga-egosi ịgụ ọkọlọtọ ịdọ aka ná ntị.
"Nwelite firmware dị." Pịa Mmelite ugbu a ka ịmalite mmelite.
Ị nwere ike view ụdị firmware arụnyere ugbu a nke gripper. Na ihuenyo mmetụ ahụ, gaa na akụrụngwa wee pịa gripper. Na ngwa Opentrons, chọta kaadị gripper n'okpuru Ngwa na Modules, pịa menu ntụpọ atọ (), wee pịa About gripper.
3.4 Pendant Kwụsị Mberede
Pendant Kwụsị Mberede (E-stop) bụ bọtịnụ ngwaike raara onwe ya nye maka ịkwụsị ngagharị robot ngwa ngwa. Opentrons Flex chọrọ ebe E-stop na-arụ ọrụ, nke ewepụrụ ka etinye ya oge niile. Mgbe ịpịrị bọtịnụ nkwụsị, Flex na-akagbu ụkpụrụ ọ bụla na-agba ọsọ ma ọ bụ nhazi ọrụ nhazi ngwa ngwa o kwere mee ma gbochie ọtụtụ ngagharị robot.
Mgbe iji E-nkwụsị
Ị nwere ike pịa E-stop:
Mgbe enwere ihe ize ndụ nke mmerụ ahụ ma ọ bụ mmerụ ahụ nye onye ọrụ. Mgbe enwere ihe egwu dị nso nke mmebi nke robot ma ọ bụ ngwaike ndị ọzọ. Mgbe samples ma ọ bụ reagents nọ n'ihe egwu dị nso nke mmetọ. Mgbe a ngwaike nkukota.
Dị ka ọ dị, ịkwesighi ịpị akara E-stop (ma e wezụga n'oge ule njirimara ngwaike na-adịkarịghị).
Ejila E-nkwụsị kagbuo ọrụ a na-atụ anya ya. Kama, jiri bọtịnụ ngwanrọ na ihuenyo mmetụ ma ọ bụ na ngwa Opentrons. Nkwụsịtụ site na ngwanrọ ga-ahapụ gị ịmaliteghachi ma ọ bụ kagbuo protocol gị, ebe ịpị E-nkwụsị na-akagbu ụkpụrụ ahụ ozugbo.

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ISI NKE 3: Nkọwa usoro

Na-etinye aka na ịtọhapụ E-stop
E-nkwụsị ahụ nwere usoro pịa-iji tinye aka, ntụgharị-na-ahapụ.
Tinye: Pịa aka na bọtịnụ uhie. Flex ga-abanye na steeti kwụsịrị. Kpebisie ike: Ozugbo kwụsịrị, dozie nsogbu ọ bụla dị n'ebe ọrụ, dị ka ikpochapụ ihe ndị awụfuru,
wepụ labware, ma ọ bụ ịkwagharị gantry (ọ kwesịrị iji aka na-agagharị n'enweghị ihe ọ bụla). Mwepụta: Tụgharịa bọtịnụ ahụ n'akụkụ elekere. Ọ ga-agbago ruo n'ọnọdụ ewepụrụ ya. Tọgharia: Na ihuenyo mmetụ ma ọ bụ na ngwa Opentrons, gosi na ị dịla njikere maka Flex ịmaliteghachi
ngagharị. Gantry ga-alaghachi n'ọnọdụ ụlọ ya na ọrụ modul ga-amaliteghachi.
Na steeti kwụsịrị, Flex na ngwaike ejikọrọ ga-eme ka ndị a:

Ngwa ngwa Gantry Pipettes
Gripper
Igwe ọkụ-Shaker Module
Okpomọkụ Module Thermocycler Ọnọdụ ìhè Touchscreen

Omume
Akwụsịla ngagharị kwụ ọtọ na akpaghị aka. A na-anabata mmegharị kehoraizin nke aka.
Akwụsịla mmegharị kwụ ọtọ nke pipettes. Igwe breeki moto na anyụike kwụ ọtọ na-etinye aka iji gbochie
pipettes si na-ada. Akwụsịla ngagharị iwe na mbuli elu.
Akwụsịla ngagharị kwụ ọtọ nke gripper. The moto breeki na vetikal axis na-aku iji gbochie
gripper si na-ada. The agba Motors na-egosipụta gripping ike na-anọgide na-enyere aka, ya mere
gripper agaghị ada labware ọ nwere ike na-ebu.
Onye na-ama jijiji na-akwụsị na ụlọ. Ihe mgbochi labware na-emepe. Agbanyụrụ ikpo ọkụ.
Agbanyụrụ ikpo ọkụ ma ọ bụ jụrụ oyi.
Agbanyụrụ ikpo ọkụ ma ọ bụ jụrụ oyi.
Ìhè ahụ na-atụgharị uhie.
Ozi kagbuo na-eweghara ihuenyo ahụ. Ihe ngosi ihuenyo na-egosi mgbe ịmechara nke ọma
wepụrụ bọtịnụ nkwụsị.

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ISI NKE 3: Nkọwa usoro
3.5 Njikọ

Mgbanwu / Gbanyụọ

Ekpuchi akụkụ

Ọdụ ụgbọ mmiri USB-A

Ntinye ike IEC

Ọdụ ụgbọ mmiri AUX-1, AUX-2, USB-B, Ethernet

Njikọ ike
Opentrons Flex na-ejikọta na isi iyi ọkụ site na ntinye IEC-C14 ọkọlọtọ. Robot nwere ihe ọkụ ọkụ AC/DC zuru oke n'ime, na-anabata 100 VAC, ntinye 240/50 Hz wee tụgharịa ya na 60 VDC. Igwe eletrọnịkị ndị ọzọ niile dị n'ime bụ 36 VDC ọkọnọ.

Ịdọ aka ná ntị: Jiri naanị eriri ọkụ enyere na robọt. Ejila eriri ọkụ na-ezughị oke ma ọ bụ voltage ratings.
Debe eriri ọkụ n'enweghị ihe mgbochi ka i wee wepụ ya ma ọ dị mkpa.

Enwekwara batrị cell mkpụrụ ego CR1220 iji mee ka elekere robot na-arụ ọrụ ozugbo mgbe ọ na-ejikọtaghị ya na ike mains. Batrị ahụ dị n'ime mkpuchi ihuenyo mmetụ ahụ. Kpọtụrụ Nkwado Opentron maka ozi ndị ọzọ ma ọ bụrụ na ị chere na ị ga-edochi batrị.

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ISI NKE 3: Nkọwa usoro
USB na njikọ inyeaka
Opentrons Flex nwere ọdụ ụgbọ mmiri USB 10 dị na mpaghara dị iche iche nke robot, nke na-eje ozi dị iche iche.
Ọdụ ụgbọ mmiri 8 azụ USB-A (ọnụọgụgụ USB-1 site na USB-8) na ọdụ ụgbọ mmiri inyeaka 2 (njikọ M12 ọnụ AUX-1 na AUX-2) bụ maka ijikọ modul na ngwa Opentrons. Hụ isi modul maka ozi ndị ọzọ gbasara ijikọ ngwaọrụ ndị a na iji ha na protocol gị. Ọdụ ụgbọ mmiri USB-B n'azụ bụ maka ijikọ robot na laptọọpụ ma ọ bụ kọmputa desktọpụ, iji guzobe nkwurịta okwu na ngwa Opentrons na-agba ọsọ na kọmputa ejikọrọ. Ọdụ ụgbọ mmiri USB-A n'ihu (USB-9), nke dị n'okpuru ihuenyo mmetụ ahụ, nwere otu ọrụ dị ka ọdụ ụgbọ mmiri USB-A n'azụ.
Mara: Ọdụ ụgbọ mmiri USB nwere oke ike iji chekwa robot na ngwaọrụ ejikọrọ. A na-ekewa nnyefe ike n'ime n'ime ọdụ ụgbọ mmiri atọ: ọdụ ụgbọ mmiri USB-A aka ekpe (USB-1 site na USB-4), ọdụ ụgbọ mmiri USB-A aka nri (USB-5 site na USB-8), na USB-A n'ihu. ọdụ ụgbọ mmiri. Otu ọ bụla n'ime ndị a ga-ebunye kacha nke 500mA na ngwaọrụ USB 2.0 dakọtara.
Njikọ netwọk
Opentrons Flex nwere ike jikọọ na netwọk mpaghara site na njikọ wired (Ethernet) ma ọ bụ ikuku (Wi-Fi).
Ọdụ ụgbọ mmiri Ethernet dị n'azụ robot. Jikọọ ya na ọdụ Ethernet ma ọ bụ gbanye netwọk gị. Ma ọ bụ, malite na ụdị robot 7.1.0, jikọọ ya ozugbo na ọdụ ụgbọ mmiri Ethernet na kọmputa gị. Modul Wi-Fi dị n'ime na-akwado netwọkụ 802.11 ac/a/b/g/n nwere eriri abụọ 2.4/5 GHz.

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ISI NKE 3: Nkọwa usoro

3.6 nkọwa sistemu

Nkọwapụta izugbe

Oghere oghere dị arọ nke akụkụ
Ihuenyo mmetụ aka
Wi-Fi eriri USB
Ntinye ike Robot igwefoto
Mains ọkọnọ voltage fluctuation Main na-enye usoro nkesa mgbanwe ugboro ole mkpịrịsị okirikiri ọkọnọ ugbu a Frame mejupụtara Ohere mejupụtara ikuku chọrọ.

87 × 69 × 84 cm / 34.25 × 27 × 33 n'ime (W, D, H)
88.5 n'arọ / 195 lb 12 ANSI/SLAS oghere dakọtara na mpaghara ọrụ
(nwere ike ịnweta pipettes) oghere 4 ọzọ maka stagAtụmatụ na labware
(dị naanị maka gripper) 7-inch LCD touchscreen nwere ọkọ na-emebi emebi na Gorilla Glass 3
802.11 ac/a/b/g/n abụọ-band (2.4/5 GHz)
100 Mbps 9 USB-A ọdụ ụgbọ mmiri 1 USB-B ọdụ ụgbọ mmiri USB 2.0 ọsọ
2MP, foto na vidiyo 100 VAC, 240 Hz, 50 60 A/1 VAC, 4.0 A/115 VAC
±10%
±5%
TN-S
6.3 A
Igwe anaghị agba nchara na CNC aluminum imewe
Window akụkụ polycarbonate mbughari na ọnụ ụzọ ihu dịkarịa ala 20 cm / 8 n'etiti unit na mgbidi

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ISI NKE 3: Nkọwa usoro

Achọrọ PC ejikọrọ

Ngwa Opentrons na-agba ọsọ na: Windows 10 ma ọ bụ mgbe e mesịrị macOS 10.10 ma ọ bụ mgbe e mesịrị Ubuntu 12.04 ma ọ bụ mgbe e mesịrị.

Nkọwapụta gburugburu ebe obibi

Ọnọdụ gburugburu ebe obibi okpomọkụ dị n'ókè mmetọ iru mmiri

Iji ime ụlọ naanị +20 ruo +25 Celsius C (a tụrụ aro) 40%, anaghị ekpokọta (atụ aro) 60 (na-adịghị emetụta mmetọ)

Maka ozi ndị ọzọ gbasara ọnọdụ gburugburu ebe obibi anabatara maka ojiji na njem, lee ngalaba ọnọdụ gburugburu ebe obibi nke isi nwụnye na mbugharị.

Asambodo

Asambodo zuru ezu Enwebeghị akwụkwọ ikike/akwadoro

CE, ETL, FCC, ISO 9001 IVD, GMP

A na-ebipụta nchịkọta nke ozi asambodo na ihe mmado dị n'azụ Flex, na nso mgba ọkụ/ gbanyụọ. Maka nkọwa zuru ezu asambodo na nrube isi ozi, lee ngalaba Nrubeisi nke Iwu na Okwu Mmalite.

Nọmba usoro n'usoro
Flex ọ bụla nwere nọmba nsonazụ pụrụ iche. Ụdị nọmba nsonazụụ ahụ na-enye ozi ndị ọzọ, gụnyere ụbọchị mmepụta nke robot. Maka example, akara akara FLXA1020231007001 ga-egosi:

OPENTRONS FLEX

ISI NKE 3: Nkọwa usoro

Ihe odide FLX A10 2023 10 07 001

Otu Ụdị Ụdị Ụdị Nkeji Ụbọchị Ọnwa

Pụtara Robot bụ Opentrons Flex. Koodu maka ụdị mmepụta nke robot. Emere robọt a n’afọ 2023. E mere rọbọt ahụ n’ọnwa Ọktoba. Emere robọt ahụ n’abalị asaa nke ọnwa. Nọmba pụrụ iche maka rọbọt emere n'otu ụbọchị.

Ị nwere ike ịchọta nọmba nsonazụ maka Flex gị:
Na akwụkwọ mmado asambodo dị n'azụ Flex, nso mgba ọkụ/ gbanyụọ. N'akụkụ azụ nke ihuenyo mmetụ ahụ (n'akụkụ ebe a na-arụ ọrụ). Na ngwa Opentrons n'okpuru Ngwaọrụ> Flex gị> Ntọala Robot> Nke di elu.

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ISI NKE 4
Modul
Opentrons Flex jikọtara ya na ọtụtụ modul ngwaike Opentrons. Modul niile bụ akụkụ nke na-eburu oghere oche, ọtụtụ robot na-ejikwa ya na njikọ USB.
Isiakwụkwọ a na-akọwa ọrụ na nkọwa anụ ahụ nke modul dakọtara na sistemụ Opentrons Flex, yana otu esi ejikọta na ịhazi ha. Maka nkọwa ndị ọzọ gbasara nhazi na ojiji modul, lelee ntuziaka maka modul nke ọ bụla. Maka nkọwa gbasara itinye modul n'ime ogbugba ndu gị, lee ngalaba Onye Mmebe Protocol nke Isi Mmepe Protocol ma ọ bụ akwụkwọ ntanetị Python Protocol API.
4.1 Modul akwadoro
Opentrons Flex dakọtara na ụdị modul Opentrons dị n'elu anọ:
Module Heater-Shaker na-enye kpo oku n'elu elu yana ịma jijiji orbital. Enwere ike ikpo ọkụ na modul ahụ ruo 95 Celsius, ma nwee ike ịma jijiji sampsite na 200 ruo 3000 rpm.
Ihe ndọta magnetik bụ ngwaọrụ na-agafe agafe nke na-ejide labware nso na magneti neodymium ya dị elu. OT-2 Magnetic Module GEN1 na GEN2, nke na-ebuli magnetị ha elu na ala n'ihe gbasara labware, anaghị akwado na Opentrons Flex.
Module okpomọkụ bụ efere efere na-ekpo ọkụ na oyi nke nwere ike idowe ọnọdụ okpomọkụ n'etiti 4 na 95 Celsius.
Thermocycler Module na-enye n'elu oche, igwe ọkụ ọkụ na-akpaghị aka zuru oke, na-eme ka akpaaka nke usoro mgbago elu na mgbada. Thermocycler GEN2 dakọtara nke ọma na gripper. Enweghị ike iji Thermocycler GEN1 na gripper, yabụ anaghị akwado ya na Opentrons Flex.
Ụfọdụ modul emebere maka OT-2 dakọtara na Flex, dị ka achịkọtara na tebụl dị n'okpuru. Ihe nrịbama na-egosi ndakọrịta, yana X na-egosi enweghị ndakọrịta.

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ISI NKE 4: MODULE

Ụdị ngwaọrụ na ọgbọ Heater-Shaker Module GEN1 Igwe Ọdụdọ Igwe Ọdụdọ GEN1 Igwe Ọdụdọ Igwe Ọdụdọ GEN2 Module Okpomọkụ GEN1 Module okpomọkụ GEN1 Thermocycler Module GEN2 Thermocycler Modul

OT-2

Flex

4.2 Sistemụ caddy modul
Emebere modul dakọtara ka ọ dabara n'ime caddies na-ejide oghere n'okpuru oche. Usoro a na-enye ohere labware n'elu modul ka ọ nọrọ nso na elu oche ahụ, ọ na-enyekwa ohere maka iji eriri USB dị n'okpuru ala ka oche ahụ na-adị mma n'oge usoro iwu gị.

Caddies maka Heater-Shaker, Okpomọkụ, na Thermocycler Modul.

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ISI NKE 4: MODULE
Iji tinye modul n'ime elu oche, a ga-ebu ụzọ tinye ya n'ime caddy modul kwekọrọ. Ụdị modul ọ bụla dakọtara nwere imewe caddy nke ya nke na-ejikọta modul na labware kpọmkwem na oche gbara ya gburugburu. (Ewepụ bụ ngọngọ magnetik, nke na-adịghị achọ ike ma ọ bụ eriri USB na-ebugharị ma si otú a na-anọdụ ala n'elu oche ahụ.) Caddies maka modul nke na-ejide otu oghere nwere ike itinye ebe ọ bụla na kọlụm 1 ma ọ bụ 3; Enwere ike itinye Thermocycler naanị na oghere A1 na B1 n'otu oge.
Na mkpokọta, iji wụnye caddy modul:
1. Wepu oghere oche ọ bụla site na ebe modul ga-aga. 2. Nọdụ ala modul n'ime ya caddy na ikesiike ya arịlịka. 3. Nyefee ike modul na eriri USB site na mkpuchi akụkụ, site na oghere oche efu, na
tinye ha na modul. 4. Debe modul caddy n'ime oghere ma gbanye ya n'ime ebe.
Maka ntuziaka nrụnye kpọmkwem, lelee Quickstart Guide ma ọ bụ Ntuziaka ntuziaka maka modul akọwapụtara. Njikọ eriri na usoro nke mgbakwunye na caddy dịgasị iche site na modul.
4.3 Module mmezi
Mgbe mbụ ị rụnyere modul na Flex, ịkwesịrị ịme nhazi nhazi ọnọdụ akpaaka. Usoro a dị ka nhazi ọkwa maka akụrụngwa, ma hụ na Flex na-aga na ebe ziri ezi maka ịrụ ọrụ protocol kacha mma. N'oge nhazigharị, Flex ga-aga n'ebe dị na ihe nkwụnye ngbanwe modul, nke yiri squares calibration nke bụ akụkụ nke oghere oche mbughari.

Ihe nkwụnye mgbanwe maka Heater-Shaker, Okpomọkụ na Modul Thermocycler.
Achọrọ nhazi modul maka modul niile arụnyere site na caddy: Heater-Shaker, Temperature, na Thermocycler Modules. Ihe ngọngọ magnetik anaghị achọ nhazigharị, ọ dịkwa njikere maka ojiji ozugbo itinye ya n'elu oche.

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ISI NKE 4: MODULE
Mgbe ịhazi modul
Flex na-akpali gị na-akpaghị aka ka ị rụọ nhazi mgbe ị jikọọ na ike na modul na-enweghị data nhazi nhazi ọ bụla echekwara. (Ị nwere ike ịchụpụ ngwa ngwa a, mana ị gaghị enwe ike iji modul mee usoro ahụ ruo mgbe i debere ya.)
Ozugbo ịmechara nhazigharị, Flex na-echekwa data mmezi yana nọmba nsonazụ modul maka ojiji n'ọdịnihu. Flex agaghị eme ka ị chegharịa ọ gwụla ma ihichapụ data nhazigharị maka modul ahụ na ntọala robot. Ị nwere ike ịgbanye ma gbanyụọ modul gị kpamkpam, ma ọ bụ ọbụna bugharịa ya na oghere oche ọzọ, na-enweghị mkpa ịmegharị ya. Ọ bụrụ na ịchọrọ ịmegharịgharị, ị nwere ike ịmalite usoro ahụ n'oge ọ bụla site na kaadị modul dị na ngwa Opentrons. (Mmegharị adịghị na ihuenyo mmetụ ahụ.)
Otu esi ahazi modul
Ntuziaka na ihuenyo mmetụ ma ọ bụ na Opentrons App ga-eduzi gị na usoro nhazi nhazi. N'ozuzu usoro ndị a bụ:
1. Kpọkọtanụ akụrụngwa achọrọ, gụnyere ihe ngbanwe nhazi modul na nyocha calibration pipette. 2. Debe ihe nkwụnye mmezi n'elu modul ma hụ na ọ bụ ọkwa zuru oke.
Ụfọdụ modul nwere ike ịchọ ka ị gbanye ihe nkwụnye ọkụ na modul. 3. Tinye ihe nyocha calibration na pipette. 4. Flex ga-emegharị ozugbo iji metụ isi ihe ụfọdụ na nkwụnye ngbanwe ma chekwaa ndị a
ụkpụrụ calibration maka ojiji n'ọdịnihu.
Ozugbo mmezi mmezi na ị wepụrụ nkwụnye na nyocha, modul ga-adị njikere maka ojiji na protocol.
Mgbe ọ bụla, ị nwere ike view ma jikwaa data nhazi modul gị na ngwa Opentrons. Gaa na Ntọala Robot maka Flex gị wee pịa taabụ Calibration.

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ISI NKE 4: MODULE
4.4 Igwe ọkụ-Shaker Module GEN1

Njirimara Heater-Shaker
NA-ekpo ọkụ na ịma jijiji
Heater-Shaker na-enye kpo oku n'elu oche yana ịma jijiji orbital. Enwere ike ikpo ọkụ na modul ahụ ruo 95 Celsius C, yana pro okpomọkụ na-esotefile:
Oke okpomọkụ: 37 °C Okpomọkụ ziri ezi: ± 95 °C na 0.5 °C Otu ọnọdụ okpomọkụ: ± 55 °C na 0.5 °Camp ọnụego: 10 Celsius C/min
Modul ahụ nwere ike ịma jijiji samples site na 200 ruo 3000 rpm, yana pro na-ama jijijifile:
Dayameta nke orbital: 2.0 mm Ntuzi akụkụ okirikiri: Oke ọsọ na-aga elekere: 200 rpm izi ezi ọsọ: ± 3000 rpm
Modul ahụ nwere latch labware kwadoro maka idobe efere na modul tupu ịma jijiji.

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ISI NKE 4: MODULE
Ihe nkwụnye ọkụ dakọtara ka achọrọ maka ịgbakwunye labware na Heater-Shaker. Enwere ike ịzụta ihe nkwụnye ọkụ ozugbo site na Opentrons na https://shop.opentrons.com. Ihe nkwụnye ọkụ dị ugbu a gụnyere:

Ihe nkwụnye ọkụ zuru ụwa ọnụ

PCR nkwụnye

Ihe nkwụnye mmiri miri emi

96 Flat Bottom Adapter

Njikwa Software
The Heater-Shaker bụ nke a na-ahazi nke ọma na Protocol Designer na Python Protocol API. Python API na-enye ohere ka emee usoro protocol ndị ọzọ n'otu oge mgbe Heater-Shaker na-arụ ọrụ. Hụ iwu anaghị egbochi n'ime akwụkwọ API maka nkọwa gbasara ịgbakwunye usoro ndị yiri ya na protocol gị.
Na-abụghị usoro iwu, ngwa Opentrons nwere ike igosipụta ọkwa dị ugbu a nke Heater-Shaker ma nwee ike ijikwa igwe kpo oku, shaker na latch labware ozugbo.

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ISI NKE 4: MODULE

Nkọwapụta ọkụ-Shaker

Akụkụ arọ Module ike ntinye ike ntinye ihe nkwụnye ọkụ Main na-enye voltage fluctuation Overvoltage Ike oriri

152 × 90 × 82 mm (L/W/H) 1.34 n'arọ 36 VDC, 6.1 A 100 VAC, 240/50 Hz ± 60% Atiri II Na-adịghị arụ ọrụ: 10 W

Ụdị: Ịma jijiji: 4 W Ịkụ ọkụ: 11 W Ịkụ ọkụ na ịma jijiji: 10 W

Ọnọdụ gburugburu ebe obibi okpomọkụ dị n'ókè iru mmiri dị elu ogo mmetọ

Kachasị: 125 W Ime ime ụlọ naanị 130 °C Ruo 20%, na-abụghị condensing Ruo 25 m n'elu oké osimiri larịị 80

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ISI NKE 4: MODULE
4.5 Mgbochi Igwe Ọdụdọ GEN1

Njirimara ngọngọ magnetik
The Opentrons Magnetic Block GEN1 bụ ihe ndọta 96-nke ọma efere. A na-eji ihe mgbochi magnetik eme ihe na ụkpụrụ ndị na-adabere na ndọta iji dọpụ ụmụ irighiri ihe na nkwusioru ma dowe ha na efere olulu mmiri mgbe a na-asachapụ, sachaa, ma ọ bụ usoro elution ndị ọzọ. Maka example, nkwadebe NGS na-akpaghị aka; na-asachapụ genomic na mitochondrial DNA, RNA, ma ọ bụ protein; na usoro mmịpụta ndị ọzọ bụ ihe niile eji eme ihe nwere ike ịgụnye ngọngọ magnetik.
Akụkụ ndọta
Igwe ndọta enweghị ike, enweghị akụrụngwa eletrọnịkị ọ bụla, ọ dịghịkwa ebuli bead ndọta elu ma ọ bụ ala na ngwọta. Olulu mmiri ndị ahụ nwere magnetik neodymium mgbanaka 96 dị elu nke edobere n'àkwà mmiri juru, nke na-enyere aka ịnọgide na-enwe ndidi n'etiti ngọngọ na pipettes ka ọ na-agba ọsọ ụkpụrụ akpaaka.
Njikwa Software
The Magnetic Block GEN1 bụ nke a na-ahazi nke ọma na Protocol Designer na Python Protocol API.

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ISI NKE 4: MODULE

N'èzí protocol, Otú ọ dị, ihuenyo mmetụ na Opentrons App amaghị na enweghị ike igosipụta ọnọdụ Magnetic Block GEN1 dị ugbu a. Nke a bụ modul enweghị ike. O nweghi ihe eletrọnịkị ma ọ bụ igwe arụrụ arụ nwere ike ikwurịta okwu na rọbọt Flex. Ị "na-achịkwa" ngọngọ ndọta site na protocols na-eji Opentrons Flex Gripper ịgbakwunye na wepụ labware na modul a.

Nkọwapụta ngọngọ magnetik

Akụkụ ịdị arọ Module ike Magnet ọkwa ọnọdụ gburugburu ebe obibi okpomọkụ dị n'ókè iru mmiri dị elu ogo mmetọ.

136 × 94 × 45 mm (L/W/H) 1.13 kg Ọ dịghị, modul bụ unpowered N52 neodymium Ime ụlọ naanị 20 °C 25%, na-abụghị condensing Ruo 30 m n'elu oké osimiri larịị 80

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ISI NKE 4: MODULE
4.6 Okpomọkụ Module GEN2

Atụmatụ modul okpomọkụ
ekpo ọkụ na oyi
The Opentrons Temperature Module GEN2 bụ modul efere dị ọkụ na oyi. A na-ejikarị ya na protocol chọrọ ikpo ọkụ, oyi, ma ọ bụ mgbanwe okpomọkụ. Modul ahụ nwere ike iru na idobe okpomọkụ sitere na 4 Celsius C ruo 95 Celsius C n'ime nkeji nkeji, dabere na nhazi modul na ọdịnaya ya.
Mgbochi okpomọkụ
Iji jide labware na okpomọkụ, modul na-eji aluminom thermal blocks. Modul na-abịa na 24well na 96-well thermal blocks. Okpomọkụ Module caddy na-abịa na ngọngọ olulu mmiri miri emi yana ngọngọ dị larịị emebere maka iji Flex Gripper. Ihe mgbochi ndị ahụ na-ejide tubes 1.5 ml na 2.0 ml, efere PCR 96 nke ọma, ibe PCR, efere olulu mmiri miri emi, na efere ala dị larịị.

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ISI NKE 4: MODULE
Mara: Modul ahụ na-ebugharị ya na ngọngọ dị larịị maka OT-2. Ejila ihe mgbochi OT-2 na Flex. Ngwongwo ala dị larịị maka Flex nwere okwu ndị a "Opentrons Flex" n'elu elu ya. Otu maka OT-2 adịghị.

24-nke ọma thermal ngọngọ

96-nke ọma thermal ngọngọ

Ihe mgbochi ikpo ọkụ dị omimi

Ọkụ ọkụ dị larịị maka Flex

Ịsa mmiri na ikpo ọkụ
N'ihi na ikuku bụ ezigbo ihe mkpuchi ọkụ, oghere dị n'etiti labware na ihe mgbochi ọkụ nwere ike imetụta arụmọrụ oge okpomọkụ nke Module okpomọkụ. Ịtụkwasị ntakịrị mmiri na 24- ma ọ bụ 96-well thermal blocks na-ewepụ oghere ikuku ma na-eme ka ọkụ ọkụ dịkwuo mma. Ọnụ mmiri dị mma na-adabere na ngọngọ thermal na labware. Hụ akwụkwọ ọcha nke Module okpomọkụ maka ntụnye ndị ọzọ.

Njikwa Software
Modul okpomọkụ bụ nke a na-ahazi nke ọma na Protocol Designer na Python Protocol API.
Na-abụghị protocol, ngwa Opentrons nwere ike igosipụta ọnọdụ ọnọdụ okpomọkụ dị ugbu a ma nwee ike ịchịkwa okpomọkụ nke efere elu.

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Nkọwapụta modul okpomọkụ

Akụkụ arọ Module ike
Ọnọdụ gburugburu ebe obibi okpomọkụ dị n'ókè iru mmiri dị elu ogo mmetọ

194 × 90 × 84 mm (L/W/H) 1.5 n'arọ Ntinye: 100 VAC, 240/50 Hz, 60 A mmepụta: 4.0 VDC, 36 A, 6.1 W max Ime ime naanị <219.6 °C (akwadoro maka ezigbo oyi. ) Ruo 22%, anaghị ekpo ọkụ ruo 60 m n'elu oke osimiri 2000

4.7 Thermocycler Modul GEN2

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Njirimara Thermocycler
The Opentrons Thermocycler Module GEN2 bụ thermocycler na-arụ ọrụ nke ọma, na-enye PCR na-enweghị aka n'ụdị efere efere 96. Ihe mkpuchi ya na-ekpo ọkụ na akara a ga-atụfu ya dabara n'elu efere ahụ, na-eme ka ọ dị mmaample kpo oku na ntakiri evaporation.
Ịkpọ oku na ime ka ọ dị jụụ Ihe mgbochi Thermocycler nwere ike ikpo ọkụ ma dị jụụ, mkpuchi ya nwere ike ikpo ọkụ, yana usoro okpomọkụ ndị a.file: Okpomọkụ ngọngọ okpomọkụ: 4 °C Thermal ngọngọ kacha kpo oku ramp ọnụego: 4.25 °C / s si GEN2 ambient ruo 95 Celsius C Thermal ngọngọ kacha jụrụ ramp ọnụego: 2.0 Celsius C / s site na 95 Celsius C ruo ihe dị n'akụkụ mkpuchi mkpuchi okpomọkụ: 37 Celsius C mkpuchi okpomọkụ ziri ezi: ± 110 ° C Enwere ike imeghe ma ọ bụ mechie mkpuchi akpaaka dị ka ọ dị mkpa n'oge mmebe protocol.
THERMOCYCLER PROFILES Thermocycler nwere ike ime profiles: ịnya igwe na-akpaghị aka site na usoro nke mgbochi okpomọkụ iji mee mmeghachi omume mmetụta okpomọkụ.
RUBBER AUTOMATION SEALS Thermocycler na-abịa na akàrà akpaaka rọba iji nyere aka belata evaporation. A ga-emerịrị akara nke ọ bụla tupu ejiri ya ma nwee ike iji ya mee ọtụtụ ọsọ. Enwere ike ịzụta akara ndị ọzọ ozugbo site na Opentrons na https://shop.opentrons.com.
Njikwa SOFTWARE Thermocycler bụ nke a na-ahazi nke ọma na Protocol Designer na Python Protocol API. Na-abụghị usoro iwu, ngwa Opentrons nwere ike igosipụta ọkwa ugbu a nke Thermocycler ma nwee ike ịchịkwa okpomọkụ mgbochi, mkpuchi mkpuchi, na ọnọdụ mkpuchi.

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Nkọwapụta thermocycler

Akụkụ (mkpuchi mepere emepe) Akụkụ (mkpuchi mechiri emechi) Ibu (gụnyere duct azụ) Ihe nkwụnye ọkụ vol.tage Power nkwụnye ọkụ ugbu a Overvoltage Ọnọdụ gburugburu ebe obibi okpomọkụ Ọdịda iru mmiri dị elu chọrọ

244.95 × 172 × 310.1 mm (L/W/H) 244.95 × 172 × 170.35 mm (L/W/H) 8.4 kg 100 V na 240/50 Hz 60 A Category II Ime ụlọ naanị 8.5 Celsius); 5 Celsius C (anabatara) 20%, na-abụghị condensing Ruo 25 m n'elu oké osimiri larịị dịkarịa ala 2 cm / 40 n'etiti unit na a mgbidi

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ISI NKE 5
Labware
Isiakwụkwọ a na-ekpuchi ihe ndị dị na Opentrons Labware Library ị nwere ike iji Opentrons Flex na Opentrons Flex Gripper. Ọ na-ekpuchikwa labware omenala yana, maka ndị ọrụ ike anyị, na-ejikọta ngwa labware na JSON ha kwekọrọ file nkọwa.
Ị nwere ike ịzụta labware n'aka ndị nrụpụta akụrụngwa mbụ ma ọ bụ site na ụlọ ahịa Opentrons na https://shop.opentrons.com. Na, Opentrons na-arụ ọrụ mgbe niile iji nyochaa nkọwa labware ọhụrụ. Hụ Ọbá akwụkwọ Labware (njikọ n'elu) maka ndepụta kacha ọhụrụ.
5.1 Echiche labware
Labware gụnyere ihe karịrị naanị ihe etinyere n'elu oche ma jiri ya na protocol. Iji nyere gị aka ịghọta Opentrons labware, ka anyị nyochaa isiokwu a site n'echiche atọ dị iche iche. Maka Opentrons Flex, labware gụnyere ihe dị n'ọbá akwụkwọ Labware anyị, data na-akọwapụta ibe labware ọ bụla, yana labware omenala.
Labware dị ka ngwaike
Ọbá akwụkwọ Labware gụnyere ihe niile ị nwere ike iji na ndabara na Opentrons Flex. Ndị a bụ ihe ndị na-adịgide adịgide na ihe ndị ị na-eri eri nke ị na-arụ ọrụ, jigharịa, ma ọ bụ tụfuo mgbe ị na-eme usoro. Ịkwesighi ịme ihe ọ bụla pụrụ iche iji rụọ ọrụ na ihe ndị dị na Labware Library. Robot Flex maara ka esi arụ ọrụ na ihe niile dị n'ọbá akwụkwọ na-akpaghị aka.
Labware dị ka data
A na-echekwa ozi ụlọ nyocha na ihe ngosi Javascript (JSON) files na .json file ndọtị. A JSON file na-agụnye akụkụ oghere (ogologo, obosara, ịdị elu), ikike volumetric (L, mL), na metric ndị ọzọ na-akọwapụta njirimara elu, ọdịdị ha, na ọnọdụ. Mgbe ị na-eme protocol, Flex na-agụ .json ndị a files ịmata ihe labware dị na oche na otu esi arụ ọrụ na ya.

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Omenala labware
Omenala labware bụ labware na-adịghị etinye n'ọbá akwụkwọ Labware ma ọ bụ bụ labware nke Onye Okike Custom Labware mepụtara. Otú ọ dị, mgbe ụfọdụ, echiche nke labware omenala na-abịa site n'echiche nke mgbagwoju anya, mmefu, ma ọ bụ ihe isi ike. Mana, labware omenala ekwesịghị isi ike nghọta ma ọ bụ mepụta. Ka anyị wepụta oge iji bupụ echiche nke labware omenala.
Dị ka exampN'ezie, Library Opentrons Labware gụnyere 96-well plates (200 L) sitere na Corning na BioRad, mana ndị na-emepụta ihe ndị ọzọ na-emekwa efere ọma ndị a. Na, n'ihi ụkpụrụ ụlọ ọrụ a na-anabatakarị, ndịiche dị n'etiti ihe ụlọ nyocha ndị a dị n'ebe niile pere mpe. Agbanyeghị, ihe nkịtị 200 L, 96-well efere sitere na Stellar Scientific, Oxford Lab, ma ọ bụ Krackerer Scientific (ma ọ bụ ndị na-eweta ihe ọ bụla maka okwu ahụ) bụ “ labware omenala” maka Flex n'ihi na akọwapụtaghị ya na ọbá akwụkwọ Labware anyị. . Na mgbakwunye, obere ọdịiche dị na akụkụ labware nwere ike inwe mmetụta dị egwu na ọganiihu nke ọsọ protocol gị. Maka nke a, ọ dị mkpa ịnwe nkọwa labware ziri ezi maka labware ọ bụla ịchọrọ iji na protocol gị.
Ọzọkwa, ebe labware omenala nwere ike ịbụ ngwa esoteric, nke na-apụ apụ, ọtụtụ oge ọ bụ naanị ndụmọdụ, efere, tubes, na racks na-eji kwa ụbọchị na ụlọ nyocha n'ụwa nile. Ọzọ, naanị ihe dị iche n'etiti Opentrons labware na labware omenala bụ ihe ọhụrụ akọwapụtaghị ya na ngwanrọ na-enye ike robot. Flex nwere ike, ma na-arụ ọrụ na ihe ndị ọzọ bụ isi labware ma ọ bụ ihe pụrụ iche, mana ịkwesịrị ịdekọ njirimara nke ihe ahụ na nkọwa labware JSON. file wee bubata data ahụ n'ime ngwa Opentrons. Hụ ngalaba nkọwa ụlọ nyocha omenala n'okpuru maka ozi ndị ọzọ.
Iji nchikota, labware gụnyere:
Ihe niile dị na Opentrons Labware Library. Nkọwa labware: data dị na JSON file nke na-akọwapụta ụdị, nha, na ike nke ihe ndị dị n'otu n'otu
dị ka nke ọma efere, ndụmọdụ, reservoirs, wdg. Custom labware, nke bụ ihe na-adịghị gụnyere na Labware Library.
Mgbe reviewN'ime echiche ndị a dị mkpa, ka anyị nyochaa edemede na ihe ndị dị na Opentrons Labware Library. Mgbe nke ahụ gasịrị, anyị ga-emecha isiakwụkwọ ahụ na njedebeview nke data components nke labware file ma chịkọta atụmatụ na ọrụ Opentrons na-enyere gị aka ịmepụta labware omenala.

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5.2 Reservoirs
Opentrons Flex na-arụ ọrụ na ndabara yana ebe nchekwa otu na ọtụtụ olulu mmiri edepụtara n'okpuru. Iji ọdọ mmiri ndị a na-enyere aka belata ibu ọrụ nkwadebe gị n'ihi na ha adịla njikere ozugbo na igbe ahụ. Ozi nchekwa nchekwa dịkwa na Opentrons Labware Library.

Otu mmiri mmiri mmiri

Nkọwapụta ndị nrụpụta

Agilent

290 ml V ala

Axygen

90 ml nke mmiri ara ehi

Akwu

195 ml nke mmiri ara ehi

290 ml V ala

API ibu aha
agilient_1_ reservoir_290ml
axygen_1_ reservoir_90ml
nest_1_ reservoir_195ml
nest_1_ reservoir_290ml

Multi-olulu mmiri

Nkọwapụta ndị nrụpụta

Akwu

12 olulu mmiri 15 mL / nke ọma V ala

USA Sayensị

12 olulu mmiri 22 mL / nke ọma V ala

Aha ibu API nest_12_ reservoir_15ml
usascientific_12_ reservoir_22ml

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Nkọwapụta mmiri mmiri na API
Ọbá akwụkwọ Opentrons Labware na-akọwapụta njirimara nke ọdọ mmiri ndị e depụtara n'elu na JSON dị iche iche files. Robot na Opentrons Python API na-adabere na nkọwa JSON ndị a iji rụọ ọrụ na labware nke usoro gị na-eji. Maka examplee, mgbe ị na-arụ ọrụ na API, ọrụ ProtocolContext.load_labware na-anabata aha labware ndị a dị ka paramita dị irè na koodu gị. Aha ibu API ejikọrọ jikọọ na nkọwapụta labware nchekwa nchekwa na ebe nchekwa Opentrons GitHub.
labware nchekwa nchekwa omenala
Gbalịa iji Opentrons Labware Onye Okike mepụta nkọwa labware omenala ma ọ bụrụ na edeghị mmiri mmiri ị ga-achọ iji mee ebe a. Nkọwa omenala na-ejikọta akụkụ niile, metadata, ụdị, ikike volumetric na ozi ndị ọzọ na JSON file. Opentrons Flex chọrọ ozi a iji ghọta otu esi arụ ọrụ na labware omenala gị. Lee ngalaba nkọwapụta ụlọ nyocha maka ozi ndị ọzọ.
5.3 Efere nke ọma
Opentrons Flex na-arụ ọrụ na ndabara yana efere ọma edepụtara n'okpuru. Iji efere ọma ndị a na-enyere aka ibelata ibu ọrụ nkwadebe gị n'ihi na ha adịla njikere ozugbo na igbe. Ozi efere ọma dịkwa na Opentrons Labware Library.

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6-efere nke ọma
Onye nrụpụta Corning

Nkọwapụta
olulu mmiri 6 16.8 mL/olulu mmiri okirikiri, ala dị larịị

Aha ibu API coring_6_wellplate_16.8ml_flat

12-efere nke ọma
Onye nrụpụta Corning

Nkọwapụta
olulu mmiri 12 6.9 mL/olulu mmiri okirikiri, ala dị larịị

Aha ibu API coring_12_wellplate_6.9ml_flat

24-efere nke ọma
Onye nrụpụta Corning

Nkọwapụta
olulu mmiri 24 3.4 mL/olulu mmiri okirikiri, ala dị larịị

Aha ibu API coring_24_wellplate_3.4ml_flat

48-efere nke ọma
Onye nrụpụta Corning

Nkọwapụta
olulu mmiri 48 1.6 mL/olulu mmiri okirikiri, ala dị larịị

Aha ibu API coring_48_wellplate_1.6ml_flat

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96-efere nke ọma
Onye nrụpụta Bio-Rad Corning NEST
Opentrons Thermo Scientific
USA Sayensị

Nkọwapụta
olulu mmiri 96 200 µL/olulu mmiri okirikiri, V ala
olulu mmiri 96 360 µL/olulu mmiri okirikiri, ala dị larịị
olulu mmiri 96 100 µL/ ọma olulu mmiri okirikiri, V ala PCR zuru oke
olulu mmiri 96 200 µL/olulu mmiri okirikiri, ala dị larịị
olulu mmiri miri emi 96 2000 µL/olulu mmiri Square, V ala
olulu mmiri siri ike 96 200 µL/ ọma olulu mmiri okirikiri, V ala PCR zuru oke
Mmiri miri emi 96 1300 µL/olulu mmiri okirikiri, U ala
Mmiri miri emi 96 2000 µL/olulu mmiri okirikiri, U ala
96 miri emi 2.4 mL / ọma Square olulu mmiri, U ala

Aha ibu API biorad_96_wellplate_200ul_pcr
coring_96_wellplate_360ul_flat
nest_96_wellplate_100ul_pcr_full_skirt
nest_96_wellplate_200ul_flat
nest_96_wellplate_2ml_deep
opentrons_96_wellplate_200ul_pcr_full_ skirt
thermoscientificnuc_96_wellplate_ 1300ul thermoscientificnuc_96_wellplate_ 2000ul usascientific_96_wellplate_2.4ml_deep

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384-efere nke ọma

Onye nrụpụta etinyere Bio-Rad Bio-Rad
Corning

Nkọwapụta
olulu mmiri 384 40 µL/olulu mmiri okirikiri, V ala
olulu mmiri 384 50 µL/olulu mmiri okirikiri, V ala
olulu mmiri 384 112 µL/olulu mmiri Square, ala dị larịị

Aha ibu API applybiosystemsmicroamp_384_ wellplate_40ul biorad_384_wellplate_50ul
coring_384_wellplate_112ul_flat

Ọfọn efere nkwụnye
Efere aluminum depụtara n'okpuru bụ ihe nkwụnye ọkụ maka Opentrons Heater-Shaker GEN1 modul. Ị nwere ike iji nkọwa nkwụnye nkwụnye ndị a kwụ ọtọ ka ibunye Opentrons kwenyesiri ike ma ọ bụ labware omenala n'elu Heater-Shaker.

Ụdị ihe nkwụnye ọkụ Opentrons Universal Flat Heater-Shaker Adapter Opentrons 96 PCR Heater-Shaker Adapter Opentrons 96 Deep Well Heater-Shaker Adapter Opentrons 96 Flat Bottom Heater-Shaker Adapter

Aha ibu API opentrons_universal_flat_adapter opentrons_96_pcr_adapter opentrons_96_deep_well_adapter opentrons_96_flat_bottom_adapter

Ị nwekwara ike ibu ma nkwụnye ọkụ na labware na otu nkọwa. Ọbá akwụkwọ Labware anyị gụnyere ọtụtụ ihe nkwụnye ọkụ ahaziri ahazi na ngwakọta labware nke na-eme Heater-Shaker dị njikere iji ozugbo na igbe ahụ.

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Mara: Ejila nkọwa jikọtara ọnụ ma ọ bụrụ na ịchọrọ ịkwaga labware na ma ọ bụ gbanyụọ Heater-Shaker n'oge protocol gị, ma ọ bụ jiri gripper ma ọ bụ aka gị. Jiri nkọwa nkwụnye kwụ ọtọ kama.

Ngwakọta nkwụnye / labware

API ibu aha

Opentrons 96 Deep Well Heater-Shaker Adapter na NEST Deep Well Plate 2 mL

opentrons_96_deep_well_adapter_nest_ wellplate_2ml_deep

Opentrons 96 Flat Bottom Heater-Shaker Adapter nwere NEST 96 Well Plate 200 µL Flat

opentrons_96_flat_bottom_adapter_nest_ wellplate_200ul_flat

Opentrons 96 PCR Heater-Shaker Adapter nwere NEST Well Plate 100 µL

opentrons_96_pcr_adapter_nest_wellplate_ 100ul_pcr_full_skirt

Opentrons Universal Flat Heater-Shaker Adapter na Corning 384 Well Plate 112 µL Flat

opentrons_universal_flat_adapter_corning_384_ wellplate_112ul_flat

Enwere ike ịzụta ihe nkwụnye ọkụ ozugbo site na Opentrons na https://shop.opentrons.com.

Efere ọma na nkọwa API
Ọbá akwụkwọ Opentrons Labware na-akọwapụta njirimara nke olulu mmiri ndị e depụtara n'elu na JSON dị iche files. Robot Flex na Opentrons Python API dabere na nkọwa JSON ndị a iji rụọ ọrụ na labware nke usoro iwu gị na-eji. Maka examplee, mgbe ị na-arụ ọrụ na API, ọrụ ProtocolContext.load_labware na-anabata aha labware ndị a dị ka paramita dị irè na koodu gị. Aha ibu API ejikọrọ jikọọ na nkọwapụta labware plate olulu mmiri na ebe nchekwa Opentrons GitHub.

Omenala ọma plate labware
Gbalịa iji Opentrons Labware Onye Okike mee nkọwapụta labware omenala ma ọ bụrụ na edepụtaghị efere ọma ị ga-achọ iji mee ebe a. Nkọwa omenala na-ejikọta akụkụ niile, metadata, ụdị, ikike volumetric na ozi ndị ọzọ na JSON file. Opentrons Flex na-agụ ozi a ka ọ ghọta ka esi arụ ọrụ na labware omenala gị. Hụ ngalaba nkọwa ụlọ nyocha maka ozi ndị ọzọ.

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5.4 Ndụmọdụ na ọnụ ahịa
Ndụmọdụ Opentrons Flex na-abịa na nha 50 µL, 200 µL na 1000 µL. Ndị a doro anya ndụmọdụ polypropylene na-adịghị eduzi nke dị na ma ọ bụ na-enweghị nzacha. A na-achịkọta ha sterile n'ime racks na-ejide ndụmọdụ 96 na enweghị DNase, RNase, protease, pyrogens, DNA mmadụ, endotoxins, na PCR inhibitors. Racks tinyekwara nọmba nza na ụbọchị ngafe.
Atụmatụ Flex pipette na-arụ ọrụ na Opentrons Flex 50 µL na 1000 µL pipettes na nhazi 1-, 8-, na 96-ọwa. Ọ bụ ezie na ị nwere ike itinye ndụmọdụ Flex ọ bụla na 50 L na 1000 L pipettes, gbalịa ka ị kwekọọ n'ọnụ ya na ikike nke pipette nwere. Maka examplee, ọ nwere ike bụrụ ihe na-adịghị mma itinye 1000 L n'ọnụ na 50 L pipette. Maka 1000 L pipette, ị nwere ike iji 50 L, 200 L, ma ọ bụ 1000 L n'ọnụ.

Ndụmọdụ racks
A na-ejikọta ndụmọdụ ndị a na-enyochabeghị na nke a kpara akpa n'ime akwa nke nwere efere ntọala a na-emegharịgharị, efere etiti nke na-ejide ndụmọdụ 96, na mkpuchi.

Ntụnye aka site na olu 50 µL 200 µL 1000 µL

API ibu aha
Achọghị: opentrons_flex_96_tiprack_50ul ekpochapụrụ: opentrons_flex_96_filtertiprack_50ul
Achọghị: opentrons_flex_96_tiprack_200ul ekpochapụrụ: opentrons_flex_96_filtertiprack_200ul
Achọghị: opentrons_flex_96_tiprack_1000ul ekpochapụrụ: opentrons_flex_96_filtertiprack_1000ul

Iji nyere aka na njirimara, akara n'ọnụ ọnụ etiti efere bụ agba dabere na nha ọnụ ọnụ:
50 µL: magenta 200 L: odo 1000 µL: acha anụnụ anụnụ

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Mgbe ị na-enye iwu ma ọ bụ na-atụgharị, ndụmọdụ na racks na-abata na nhazi ngwugwu abụọ dị iche iche:
Racks: Ihe mejupụtara nke sọọsọ n'ọnụ ọnụ kpuchiri ekpuchi (efere ala, efere etiti nwere ndụmọdụ, na mkpuchi). Nhazi echekwabara kacha mma mgbe ịdị ọcha dị oke mkpa, iji zere mmetọ obe, ma ọ bụ mgbe protocol gị anaghị ekwe ka e jiri ya mee efere ma ọ bụ ihe mejupụtara ya.
Ntugharị: Mejupụtara otu akwa ntụ ntụ zuru oke (efere ala, efere etiti nwere ndụmọdụ, na mkpuchi) yana arịa ọnụ n'otu n'otu. Nhazi njupụta kacha mma mgbe protocol gị na-enye ohere ka e jiri ya mee ihe n'ala ala ma ọ bụ ihe mejupụtara ya.
Ndakọrịta Tippipet
Emebere ndụmọdụ pipette Flex maka Opentrons Flex pipettes. Atụmatụ Flex adịghị azụ azụ dakọtara na Opentrons OT-2 pipettes, ma ọ bụ ị nweghị ike iji ndụmọdụ OT-2 na Flex pipettes.
Atụmatụ ụkpụrụ ụlọ ọrụ ndị ọzọ nwere ike ịrụ ọrụ na Flex pipettes, mana akwadoghị nke a. Iji hụ na ịrụ ọrụ kacha mma, ị ga-eji naanị ndụmọdụ Opentrons Flex na Flex pipettes.
Ntụpọ nkwụnye ọkụ
Pipette nke ọwa 96 chọrọ ihe nkwụnye ọkụ iji tinye ntụmadị zuru oke nke ọma. N'oge usoro ntinye aka, pipette na-agagharị n'elu nkwụnye ahụ, na-agbada onwe ya na ntụtụ na-arị elu, ma na-adọta ndụmọdụ n'elu pipettes site n'ibuli ihe nkwụnye na n'ọnụ ọnụ.

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Mara: Naanị jiri ihe nkwụnye ọkụ n'ọnụ mgbe ị na-ebuli ndụmọdụ zuru ezu otu oge. Tinye ntụnye ọnụ ozugbo n'elu oche mgbe ị na-ebuli ndụmọdụ ole na ole.
Ịdọ aka ná ntị: tuo ihe ize ndụ. Wepu aka na nkwụnye ihe nkwụnye n'ọnụ mgbe pipette na-ejikọta ndụmọdụ pipette.

Ụdị ihe nkwụnye ọkụ Opentrons Flex 96 Tip Rack Adapter

Aha ibu API opentrons_flex_96_tiprack_adapter

Ihe nkwụnye ọkụ n'ọnụ dakọtara na Opentrons Flex Gripper. Ị nwere ike iji gripper na-edobe racks ọhụrụ na nkwụnye ọkụ ma ọ bụ bulite ma bugharịa racks eji eme ihe n'ime ụlọ mkpofu.

5.5 tubes na tube racks

Usoro Opentrons 4-in-1 Tube Rack na-arụ ọrụ na Opentrons Flex na ndabara. Iji 4-in-1 tube rack na-enyere aka belata ibu ọrụ nkwadebe gị n'ihi na nchikota ọ na-enye adịla njikere ozugbo na igbe ahụ. Ozi ndị ọzọ dịkwa na Opentrons Labware Library.

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Ngwakọta tube na rack
The Opentrons 4-in-1 tube rack na-akwado ụdị tube dị iche iche, otu ma ọ bụ n'ụdị dị iche iche (olu). Ndị a na-agụnye: 6-tube rack maka 50 ml tubes (6 x 50 ml). Nchikota 10-tube maka tubes 50 ml anọ na tubes 15 mL isii (4 x 50 ml, 6 x 15 ml). 15-tube akwa maka tubes 15 ml (15 x 15 ml). 24-tube akwa maka 0.5 ml, 1.5 mL, ma ọ bụ 2 mL tubes (24 x 0.5 ml, 1.5 ml, 2 ml).
Rịba ama: Ọkụ niile bụ cylindrical nwere ala V nwere ụdị (conical) ma ọ bụrụ na egosighi ya.

6-tube akwa
Ụdị tube 6 Falcon 50 ml 6 Akwu 50 ml
10-tube akwa
Ụdị tube 4 Falcon 50 ml 6 Falcon 15 ml 4 Akwu 50 ml 6 Akwu 15 ml

Aha ibu API opentrons_6_tuberak_falcon_50ml_conical opentrons_6_tuberack_nest_50ml_conical
API load name opentrons_10_tuberack_falcon_4x50ml_6x15ml_conical opentrons_10_tuberack_nest_4x50ml_6x15ml_conical

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15-tube akwa
Ụdị tube 15 Falcon 15 ml 15 Akwu 15 ml

Aha ibu API opentrons_15_tuberak_falcon_15ml_conical opentrons_15_tuberack_nest_15ml_conical

24-tube akwa

Ụdị tube
24 Eppendorf Nchekwa Mkpọchi 1.5 mL 24 Eppendorf Nchekwa mkpọchi 2 ml, U-ụdị ala 24 jeneriki 2 mL ịghasa okpu 24 NEST 0.5 mL ịghasa okpu 24 NEST 1.5 mL screw cap 24 NEST 1.5 mL screw cap 24 mL 2 NEST 24 mL snap cap 2 mL NEST XNUMX mL okpu snap, ala U-dị ka

Aha ibu API opentrons_24_tuberack_eppendorf_1.5ml_safelock_snapcap opentrons_24_tuberack_ependorf_2ml_safelock_snapcap
opentrons_24_tuberack_generic_2ml_screwcap opentrons_24_tuberack_nest_0.5ml_screwcap opentrons_24_tuberack_nest_1.5ml_screwcap opentrons_24_tuberack_nest_1.5ml_snapcap opentrons_24_tuberack_nest_2ml_screwcap opentrons_24_tuberack_nest_2ml_snapcap

Nkọwa tube rack API
Ọbá akwụkwọ Opentrons Labware na-akọwapụta njirimara nke racks tube depụtara n'elu na JSON dị iche iche files. Robot Flex na Opentrons Python API dabere na nkọwa JSON ndị a iji rụọ ọrụ na labware nke usoro iwu gị na-eji. Maka examplee, mgbe ị na-arụ ọrụ na API, ọrụ ProtocolContext.load_labware na-anabata aha labware ndị a dị ka paramita dị irè na koodu gị. Aha ibu API ejikọrọ jikọọ na nkowa tube rack labware na ebe nchekwa Opentrons GitHub.

OPENTRONS FLEX

ISI NKE 5: LABWARE
Omenala tube ogbe labware
Gbalịa ịmepụta nkọwa labware omenala site na iji Opentrons Labware Creator ma ọ bụrụ na ewepụtaghị eriri tube na rack ị ga-achọ iji mee ebe a. Nkọwa omenala na-ejikọta akụkụ niile, metadata, ụdị, ikike volumetric na ozi ndị ọzọ na JSON file. Opentrons Flex na-agụ ozi a ka ọ ghọta ka esi arụ ọrụ na labware omenala gị. Lee ngalaba nkọwapụta ụlọ nyocha maka ozi ndị ọzọ.
5.6 Aluminom blocks
Aluminom na-egbochi ụgbọ mmiri na Module okpomọkụ GEN2 ma nwee ike zụta ya iche dị ka ihe atọ. Ntọala ahụ na-agụnye efere ala dị larịị, ngọngọ nke ọma 24, na ngọngọ 96.
Opentrons Flex na-eji aluminom blocks jide sample tubes na olulu mmiri na Module okpomọkụ ma ọ bụ ozugbo na oche. Mgbe ejiri ya na Module Okpomọkụ, ihe mgbochi aluminom nwere ike idowe sample tubes, PCR ibe, ma ọ bụ efere na okpomọkụ mgbe niile n'etiti 4 Celsius C na 95 Celsius C.
Efere ala dị larịị
Efere ala dị larịị maka ụgbọ mmiri Flex nwere cadady Module Temperature yana dakọtara na efere olulu mmiri ọkọlọtọ ANSI/SL dị iche iche. Efere a dị larịị dị iche na efere nke na-ebu Module okpomọkụ n'onwe ya ma ọ bụ ihe dị iche iche nwere akụkụ atọ. Ọ na-egosipụta elu na-arụ ọrụ sara mbara yana mkpachị nkuku chamfered. Atụmatụ ndị a na-enyere aka melite arụmọrụ Opentrons Flex Gripper mgbe ị na-ebuga labware na ma ọ bụ gbanyụọ na efere.
Ị nwere ike ịkọ nke efere ala dị larịị ị nwere n'ihi na nke Flex nwere okwu "Opentrons Flex" n'elu elu ya. Otu maka OT-2 adịghị.

OPENTRONS FLEX

ISI NKE 5: LABWARE

24-nke ọma aluminum ngọngọ
A na-eji ngọngọ olulu mmiri 24 na onye sample vials. Maka example, ọ na-anabata sample vials na:
Nwee ala nke nwere ụdị V ma ọ bụ U. Chekwaa ọdịnaya site na iji okpu snap ma ọ bụ mkpọchi mkpuchi. Jide mmiri mmiri na ike nke 0.5 ml, 1.5 ml, na 2 ml.

96-nke ọma aluminum ngọngọ
Ihe mgbochi 96 nke ọma na-akwado ụdị efere dị iche iche nke ọma. Maka example, ọ na-anabata efere ọma ndị bụ:
Site na ndị na-emepụta efere ọma dị ka Bio-Rad na NEST.
Emebere ya na ala nke nwere ụdị V, ala U-ma ọ bụ ala dị larịị.
Ejiri olulu mmiri 100 µL ma ọ bụ 200 µL rụọ ya.
Ọ dakọtara na ibe PCR jeneriki.

Ihe nkwụnye kwụ ọtọ
Thermal ngọngọ Flex flat ala efere 24-ọma aluminum block 96-ọma aluminum block

Aha ibu API opentrons_aluminum_flat_bottom_plate Lee nchikota labware n'okpuru. opentrons_96_well_aluminum_block

Ngwakọta aluminom ngọngọ labware
Ọbá akwụkwọ Opentrons Labware na-akwado nchikota ngọngọ, vial na nke ọma efere ndị a, nke akọwakwara na nkọwapụta labware JSON dị iche. files. Robot Flex na Opentrons Python API

OPENTRONS FLEX

ISI NKE 5: LABWARE
dabere na nkọwapụta JSON ndị a iji rụọ ọrụ na labware nke ụkpụrụ gị na-eji. Maka examplee, mgbe ị na-arụ ọrụ na API, ọrụ ProtocolContext.load_labware na-anabata aha labware ndị a dị ka paramita dị irè na koodu gị. Tebụlụ ndị dị n'okpuru depụtara mkpokọta ngọngọ/akpa na aha ibu API metụtara ya. Njikọ jikọtara na nkọwa JSON kwekọrọ na ebe nchekwa Opentrons GitHub.
Mara: Ọkụ niile nwere ala nwere ụdị V ọ gwụla ma egosighi ya.

Ngwakọta 24-nke ọma aluminum block labware

24-nke ọma ngọngọ Generic 2 mL ịghasa okpu NEST 0.5 mL ịghasa okpu NEST 1.5 mL ịghasa okpu NEST 1.5 mL snap okpu NEST 2 mL ịghasa okpu NEST 2 mL snap okpu, U-ọdịdị ala

Aha ibu API opentrons_24_aluminumblock_generic_2ml_screwcap opentrons_24_aluminumblock_nest_0.5ml_screwcap opentrons_24_aluminumblock_nest_1.5ml_screwcap opentrons_24_aluminumblock_nest_p1.5ml_screwcap_24ml crewcap opentrons_2_aluminumblock_nest_24ml_snapcap

Ngwakọta 96-nke ọma aluminum block labware

96-nke ọma mgbochi ọdịnaya Bio-Rad ọma efere 200 µL Generic PCR strip 200 µL NEST nke ọma efere 100 µL

Aha ibu API opentrons_96_aluminumblock_biorad_wellplate_200uL opentrons_96_aluminumblock_generic_pcr_strip_200uL opentrons_96_aluminumblock_nest_wellplate_100uL

OPENTRONS FLEX

ISI NKE 5: LABWARE

5.7 Labware na Opentrons Flex Gripper
Ọ bụ ezie na Opentrons Flex na-arụ ọrụ na ngwaahịa niile dị na Labware Library, Opentrons Flex Gripper dakọtara na naanị ihe labware akọwapụtara. Ugbu a, a na-ahazi Gripper maka iji ya na ihe labware ndị a.

Ụdị efere ụlọ nyocha miri emi nke ọma 96 dị mma nke ọma
Ndụmọdụ Racks (ndụmọdụ na-enweghị nzacha na nzacha)

Ụdị
NEST 96 miri mmiri efere 2 ml
Opentrons siri ike 96 Well Plate 200 µL PCR Full Skirt NEST 96 Well Plate 200 µL Flat
Opentrons Flex 96 Atụmatụ Rack 50 µL Opentrons Flex 96 Ndụmọdụ Rack 200 µL Opentrons Flex 96 Atụmatụ Rack 1000 µL

Mara: Maka nsonaazụ kacha mma, jiri Flex Gripper naanị yana labware edepụtara n'elu. Flex Gripper nwere ike ịrụ ọrụ na ANSI/SLAS akpaaka ndị ọzọ na-akwado labware, mana akwadoghị nke a.

5.8 Nkọwa labware omenala
Dịka a tụlere na mbido isiakwụkwọ a, labware omenala bụ labware nke edepụtaghị n'ọbá akwụkwọ Opentrons Labware. Ị nwere ike iji ihe labware ndị ọzọ a na-ahụkarị ma ọ bụ pụrụ iche na Flex site na ịlele na ịdekọ njirimara nke ihe ahụ na ịchekwa data ahụ na JSON. file. Mgbe ebubatara na ngwa ahụ, Flex na API na-eji data JSON ahụ na-emekọrịta ihe na labware gị. Opentrons na-enye ngwaọrụ na ọrụ, nke anyị ga-enyocha n'okpuru, iji nyere gị aka iji Flex na labware omenala.

OPENTRONS FLEX

ISI NKE 5: LABWARE

Ịmepụta nkọwa labware omenala
Ngwa na ọrụ Opentrons na-enyere aka itinye labware omenala n'aka gị. Atụmatụ ndị a na-anabata ọkwa nka dị iche iche na ụzọ ọrụ. Ịmepụta labware nke gị, na iji ya na Opentrons Flex, na-enyere aka mee ka robot bụrụ ihe mgbakwunye na ike dị ike na ụlọ nyocha gị.

Onye Okike LABWARE dịpụrụ adịpụ
Onye Okike Labware Custom bụ koodu enweghị, webNgwá ọrụ dabere na-eji interface eserese iji nyere gị aka ịmepụta nkọwa labware file. Onye Okike Labware na-ewepụta nkọwa labware JSON file nke ị na-ebubata n'ime ngwa Opentrons. Mgbe nke ahụ gasịrị, labware omenala gị dị maka Flex robot na Python API.

Ọrụ LABWARE omenala
Kpọtụrụ anyị ma ọ bụrụ na labware nke ịchọrọ iji adịghị n'ọbá akwụkwọ ahụ, ọ bụrụ na ịnweghị ike ịmepụta nkọwa nke gị, ma ọ bụ n'ihi na ihe omenala na-agụnye ụdị dị iche iche, nha, ma ọ bụ mmebi iwu ndị ọzọ akọwara n'okpuru.

Labware ị nwere ike ịkọwa n'ime Onye Okike Labware
; Olulu mmiri na tubes bụ otu na otu. ; Ahịrị niile nwere nha nha nhata
(Oghere dị n'etiti ahịrị hà nhata).
; Ogidi niile na-agbakọ n'otu n'otu (oghere dị n'etiti kọlụm hà nhata).
; Dabara nke ọma n'otu oghere oche.

Labware Opentrons kwesịrị ịkọwa; Olulu mmiri na ụdị tube dị iche iche. ; Ahịrị anaghị agbasa nke ọma.
; A naghị agbasa ogidi n'otu n'otu.
; Obere obere oghere oche (chọrọ ihe nkwụnye ọkụ) ma ọ bụ gbasaa ọtụtụ oghere oche.

OPENTRONS FLEX

ISI NKE 5: LABWARE
Nke a bụ ụfọdụ eserese na-enyere gị aka iji anya nke uche hụ onye bụbuamples kọwara n'elu. A na-agbakọ kọlụm niile nke ọma na ahịrị niile na-agbakwa n'otu n'otu. Ogidi adịghị mkpa inwe oghere dị ka ahịrị.
Mgbe niile Grid agaghị adị n'etiti etiti labware.
Ahịrị ndị na-adịghị agafe agafe na-ekekwa nke ọma mana ogidi anaghị agbasa nke ọma.
Ogidi/ ahịrị ndị na-adịghị agafe agafe na-ekewa nke ọma mana olulu mmiri adịghị otu.
Na-adịghị mma Enwere ihe karịrị otu grid.
Ndị otu labware anyị ga-arụ ọrụ ịghọta mkpa gị yana chepụta nkọwa labware maka gị. Hụ akụkọ nkwado na-arịọ nkọwapụta labware omenala yana ụdị arịrịọ labware maka ozi ndị ọzọ. Nke a bụ ọrụ dabere na ego.

OPENTRONS FLEX

ISI NKE 5: LABWARE

Python API
Ọ bụ ezie na ịnweghị ike iji API anyị mepụta labware omenala, ị nwere ike iji labware omenala na ụzọ API dị. Agbanyeghị, ịkwesịrị ibu ụzọ kọwapụta labware omenala gị wee bubata ya na ngwa Opentrons. Ozugbo ị gbakwunyere labware gị na ngwa Opentrons, ọ dị na Python API na robot. Hụ ngalaba nkọwa ụlọ nyocha omenala nke akwụkwọ Python API maka ozi ndị ọzọ. Maka ozi gbasara iji API dee script protocol, hụ ngalaba Python Protocol API na isi mmepe Protocol.
JSON labware atụmatụ
A JSON file bụ ụkpụrụ maka ọkọlọtọ Opentron na labware omenala. Nke a file nwere ma na-ahazi data labware dịka nkọwapụta nhazi nke schema nke ndabara si dị.
A schema bụ etiti

Akwụkwọ / akụrụngwa

opentrons FLEX FLEX Opentrons Flex Open Source Liquid Handling Robot [pdf] Akwụkwọ ntuziaka
FLEX Opentrons Flex Open Source Liquid Handling Robot, FLEX, Opentrons Flex Open Source Liquid Handling Robot, Flex Open Source Liquid Handling Robot, Open Source Liquid Handling Robot, Robot Liquid Handling Robot, Robot Handling Liquid, Robot Handling

Ntụaka

Akwụkwọ ntuziaka onye ọrụ

FLEX Opentrons Flex Open Source Liquid Handling Robot

Opentrons Flex

Nkọwapụta:

Ntuziaka ojiji:

1. Nwụnye na nhazi akụrụngwa:

2. Mbugharị:

3. Njikọ:

4. Onye Mmebe Protocol:

5. Python Protocol API:

6. OT-2 Usoro:

Ajụjụ A na-ajụkarị (FAQ):

Ajụjụ: Kedu ka m ga-esi chọpụta nsogbu ma ọ bụrụ na robot anaghị emegharị dị ka
tụrụ anya?

Ajụjụ: Enwere m ike iji pipettes omenala na Opentrons Flex?

Akwụkwọ / akụrụngwa

Ntụaka

Hapụ ikwu

Kagbuo nzaghachi

FLEX Opentrons Flex Open Source Liquid Handling Robot

Opentrons Flex

Nkọwapụta:

Ntuziaka ojiji:

1. Nwụnye na nhazi akụrụngwa:

2. Mbugharị:

3. Njikọ:

4. Onye Mmebe Protocol:

5. Python Protocol API:

6. OT-2 Usoro:

Ajụjụ A na-ajụkarị (FAQ):

Ajụjụ: Kedu ka m ga-esi chọpụta nsogbu ma ọ bụrụ na robot anaghị emegharị dị ka tụrụ anya?

Ajụjụ: Enwere m ike iji pipettes omenala na Opentrons Flex?

Akwụkwọ / akụrụngwa

Ntụaka

Ozi ndị metụtara

Hapụ ikwu

Kagbuo nzaghachi

Ajụjụ: Kedu ka m ga-esi chọpụta nsogbu ma ọ bụrụ na robot anaghị emegharị dị ka
tụrụ anya?