Zofotokozera: Lorem ipsum dolor sit
amet, consectetur adipiscing elit. Yesetsani kuchita zochitika za tempor
ut labore et dolore magna aliqua.
Zofotokozera Zachilengedwe: Lorem ipsum
dolor sit amet, consectetur adipiscing elit. Sed kuchita eiusmod tempor
incididunt ut labore et dolore magna aliqua.

Zitsimikizo: Lorem ipsum dolor sit amet,
consectetur adipiscing elit. Sed kuchita eiusmod tempor incididunt ut
labore et dolore magna aliqua.
Nambala ya siriyo: XXX-XXXX-XXXX

Malangizo Ogwiritsira Ntchito Zogulitsa:

1. Kuyika ndi kusanja kwa zida:

Tsatirani ndondomeko zomwe zafotokozedwa m'buku la pipette ndi gripper
kukhazikitsa.

2. Kusamuka:

Kuti mumve zachidule, onani gawo 2.5 mu bukhuli. Za
mayendedwe akutali, tsatirani malangizo omwe aperekedwa. General kusuntha
malangizo aliponso.

3. Zolumikizana:

Onetsetsani kuti pali kulumikizana koyenera kwamagetsi monga momwe zafotokozedwera m'bukuli.
Lumikizani USB ndi zida zothandizira ngati pakufunika. Ma network
ziyenera kukhazikitsidwa potsatira malangizo.

4. Wopanga Protocol:

Mvetsetsani zofunikira za Protocol Designer ndikuphunzira momwe mungachitire
kuti mupange ma protocol atsopano kapena kusintha omwe alipo malinga ndi labu yanu
zofunika.

5. Python Protocol API:

Onani zolemba ndi kugwiritsa ntchito Python Protocol API.
Dziwani zambiri za Python zomwe zimawonjezera magwiridwe antchito.

6. OT-2 Protocols:

Phunzirani za OT-2 Python protocols, OT-2 JSON protocols, ndi
Ma protocol a Magnetic Module amitundu yosiyanasiyana yoyesera.

Mafunso Ofunsidwa Kawirikawiri (FAQ):

Q: Kodi ndimathetsa bwanji ngati loboti silikuyenda ngati
kuyembekezera?

A: Yang'anani kugwirizana kwa magetsi, onetsetsani kuti mwasintha bwino
zida, ndikuwonetsetsa kuti palibe zopinga mu
njira ya robot.

Q: Kodi ndingagwiritse ntchito ma pipette ndi Opentrons Flex?

A: Opentrons amalimbikitsa kugwiritsa ntchito ma pipette ogwirizana kuti akhale abwino
ntchito ndi kulondola.

"``

View Fullscreen

Opentrons Flex
Buku la Malangizo
Malingaliro a kampani Opentrons Labworks Inc.
Disembala 2023

© OPENTRONS 2023 Opentrons FlexTM (Opentrons Labworks, Inc.) Mayina olembetsedwa, zizindikiro zamalonda, ndi zina zotere zomwe zagwiritsidwa ntchito m'chikalatachi, ngakhale zitakhala zosazindikirika motero, siziyenera kuonedwa ngati zosatetezedwa ndi lamulo.

M'ndandanda wazopezekamo
Mawu Oyamba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 Kapangidwe ka bukuli . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Zolemba ndi machenjezo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Mutu 1: Mawu Oyamba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 1.1 Takulandilani ku Opentrons Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Zatsopano mu Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Flex workstations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 1.2 Zambiri zachitetezo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Zizindikiro zachitetezo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Machenjezo okhudza chitetezo chamagetsi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Machenjezo owonjezera otetezedwa . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 Chenjezo lachitetezo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 Kutetezedwa kwachilengedwe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Fuko lapoizoni . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Zakumwa zoyaka moto . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 1.3 Kutsata malamulo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Chitetezo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Kugwirizana kwamagetsi. . . . . . . . . . . . . . . . . . . . . . . . .20 machenjezo ndi zolemba za FCC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20 Canada kutsatira ISED. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Chenjezo la chilengedwe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Wi-Fi precertification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Mutu 2: Kuyika ndi Kusamutsa . . . . . . . . . . . . . . . . . . . . . . . 22 2.1 Zofunikira pachitetezo ndi magwiridwe antchito. . . . . . . . . . . . . . . . . . . . .22 Komwe mungayike Opentrons Flex . . . . . . . . . . . . . . . . . . . . . . . .22 Kugwiritsa ntchito mphamvu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 Mikhalidwe ya chilengedwe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 2.2 Unboxing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Khama ndi nthawi yofunikira . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Krete ndi kulongedza zinthu . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Zinthu zamalonda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 Gawo 1: Chotsani crate. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27 Gawo 2: Tulutsani Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29 Gawo 3: Msonkhano womaliza ndi mphamvu pa . . . . . . . . . . . . . . . . . . .32

2.3 Kuthamanga koyamba. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Yatsani mphamvu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Lumikizani ku netiweki kapena kompyuta. . . . . . . . . . . . . . . . . . . . .36 Ikani zosintha zamapulogalamu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37 Gwirizanitsani Pendant Yoyimitsa Mwadzidzi . . . . . . . . . . . . . . . . . . . . . . 38 Patsani dzina loboti yanu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
2.4 Kuyika ndi kusanja zida. . . . . . . . . . . . . . . . . 38 Kuyika kwa Pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 Kuyika kwa Gripper. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
2.5 Kusamuka. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 Mayendedwe aafupi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Kuyenda mtunda wautali . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Malangizo onse osuntha . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 Malingaliro omaliza okhudza kusamuka. . . . . . . . . . . . . . . . . . . . . . . . . . .44
Mutu 3: Kufotokozera Kwadongosolo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 3.1 Zigawo zathupi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45 Mafelemu ndi mpanda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 Sitimayo ndi malo ogwirira ntchito. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 Staging area. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47 Zosintha zapadenga. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 Mpandamachokero. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49 Stagmalo mipata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49 Dongosolo la kayendedwe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 Touchscreen ndi zowonetsera za LED. . . . . . . . . . . . . . . . . . . . . . . . .51 3.2 Pipettes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 Pipette zofotokozera. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Pipette calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Pipette tip rack adaputala. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Kujambula pang'ono nsonga. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 Pipette masensa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 Pipette zosintha za firmware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 3.3 Chophimba . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57 Zolemba za Gripper. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Gripper calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Gripper zosintha za firmware. . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 3.4 Choyimira Choyimitsa Mwadzidzidzi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 Nthawi yogwiritsira ntchito E-stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 Kuchita ndi kutulutsa E-stop . . . . . . . . . . . . . . . . . . . . .60

3.5 Zogwirizana. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 Kulumikizana kwamphamvu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 USB ndi maulumikizidwe othandizira. . . . . . . . . . . . . . . . . . . . . . . . .62 Kulumikizana ndi maukonde. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
3.6 Mafotokozedwe a dongosolo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63 Kufotokozera mwachidule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63 Mafotokozedwe a chilengedwe . . . . . . . . . . . . . . . . . . . . . . . . . .64 Zitsimikizo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 Nambala ya seri. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64
Mutu 4: Ma modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 4.1 Ma module othandizira. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66 4.2 Module caddy system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 4.3 Kuwongolera ma module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68 Nthawi yosinthira ma module. . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 Momwe mungasankhire ma module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 4.4 Wotentha Wotentha Module GEN1 . . . . . . . . . . . . . . . . . . . . . . . . . .70 Mawonekedwe a Heater-Shaker. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70 Zosintha za Heater-Shaker . . . . . . . . . . . . . . . . . . . . . . . . . .72 4.5 Block Magnetic GEN1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 Mawonekedwe a Magnetic Block. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 Zolemba za Magnetic Block. . . . . . . . . . . . . . . . . . . . . . . . .74 4.6 Kutentha Module GEN2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .75 Kutentha kwa Module . . . . . . . . . . . . . . . . . . . . . . . . .75 Kutentha kwa Module . . . . . . . . . . . . . . . . . . . .77 4.7 Thermocycler Module GEN2. . . . . . . . . . . . . . . . . . . . . . . . . . . .77 Thermocycler mbali . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .78 Mafotokozedwe a Thermocycler. . . . . . . . . . . . . . . . . . . . . . . . . . .79
Mutu 5: Labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 5.1 Malingaliro a Labwa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Labware ngati hardware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Labware monga deta . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Labure mwamakonda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81 5.2 Madamu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Malo osungira chitsime chimodzi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Malo osungira zitsime zambiri. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Reservoirs ndi matanthauzo a API . . . . . . . . . . . . . . . . . . . . . . . . 83 Mwambo reservoir labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 5.3 Ziwiya mbale . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

6-chitsime mbale. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 12-chitsime mbale . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 24-chitsime mbale . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 48-chitsime mbale. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 96-chitsime mbale . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 384-chitsime mbale . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 Ma adapter mbale bwino. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 Well plates ndi matanthauzo a API. . . . . . . . . . . . . . . . . . . . . . . . .87 Mwambo bwino mbale labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 5.4 Malangizo ndi zida zopangira nsonga . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 Zopangira zopangira. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 Kugwirizana kwa Tippipette. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 Adaputala yopangira nsonga. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 5.5 Machubu ndi ma chubu rack. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90 Kuphatikizika kwa chubu ndi rack. . . . . . . . . . . . . . . . . . . . . . . . . . . 91 6-machubu rack. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 10-chubu rack. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 15-machubu rack. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 24-machubu rack. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Tube rack API matanthauzo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Mwambo chubu pachiyika labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 5.6 midadada ya aluminiyamu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 Chipinda chapansi chathyathyathya. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 24-chitsime cha aluminiyamu chipika. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 96-chitsime cha aluminiyamu chipika. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Ma adapta odziyimira pawokha . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Aluminium block labware kuphatikiza. . . . . . . . . . . . . . . .94 24-chitsime cha aluminiyamu block labware kuphatikiza. . . . . . . . .95 96-chitsime cha aluminiyamu block labware kuphatikiza. . . . . . . . .95 5.7 Labware ndi Opentrons Flex Gripper . . . . . . . . . . . . . . .96 5.8 Matanthauzo amtundu wa labware . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 Kupanga matanthauzo a labware . . . . . . . . . . . . . . . . . . .97 JSON labware schema . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99 JSON matanthauzo a labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104 Mutu 6: Kupititsa patsogolo ndondomeko. . . . . . . . . . . . . . . . . . . . . . . . . . .105 6.1 Ndondomeko zopangiratu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105 Protocol Library . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105 Custom Protocol Development Service. . . . . . . . . . . . . . . .

6.2 Wopanga Protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109 Zofunikira za Wopanga Protocol. . . . . . . . . . . . . . . . . . . . . . 109 Kupanga ndondomeko . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Kusintha ma protocol omwe alipo . . . . . . . . . . . . . . . . . . . . . . . . . . 114
6.3 Python Protocol API . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 Kulemba ndi kuyendetsa zolemba . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 Python-zokhazokha. . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
6.4 OT-2 ma protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117 OT-2 Python protocols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118 OT-2 ma protocol a JSON. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120 Magnetic Module protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Mutu 7: Mapulogalamu ndi Ntchito. . . . . . . . . . . . . . . . . . . . . . . . . . 121 7.1 Kugwira ntchito pazenera. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 Dashboard ya robot. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 Protocol Management. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122 Zambiri za Protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124 Yambitsani kukhazikitsa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127 Labware Position Check. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128 Kuthamanga patsogolo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130 Kumaliza kumaliza. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131 Kasamalidwe ka zida. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132 Zokonda pa robot. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133 Kukonzekera kwa Deck. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136 7.2 Opentrons App. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 Kukhazikitsa kwa pulogalamu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 Kusamutsa ma protocol ku Flex. . . . . . . . . . . . . . . . . . . . . . . . . 140 Ma module ndi zowongolera. . . . . . . . . . . . . . . . . . . . . . . . . . . 142 Protocol yaposachedwa imayendetsa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143 7.3 Kuchita bwino kwambiri. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144 Jupyter Notebook . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144 Kugwira ntchito pamzere wolamula pa SSH. . . . . . . . . . . . . . . . . . . . 145
Mutu 8: Kusamalira ndi Utumiki . . . . . . . . . . . . . . . . . . . . . . . .147 8.1 Kuyeretsa Flex yanu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Musanayambe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Zimene mungathe kuyeretsa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 Njira zoyeretsera. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148 Kuyeretsa mafelemu ndi mazenera. . . . . . . . . . . . . . . . . . . . 148 Kutsuka masitepe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148

Kuyeretsa gantry. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 Kuyeretsa zinyalala. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 8.2 Kuyeretsa mapaipi ndi malangizo. . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 Pipette decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150 Kuyeretsa nsonga za pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151 8.3 Kuyeretsa chogwirira . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152 8.4 Ma module oyeretsa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 General module kuyeretsa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 Zisindikizo za Thermocycler. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 8.5 Autoclave-safe labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154 8.6 Kutumikira Flex. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 Ntchito za Opentrons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 Kuyenerera kwa kukhazikitsa ndi kuyenerera kwa ntchito. . . 155 Kukonzekera kodzitetezera . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156 chitsimikizo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157 Zakumapeto A: Kafotokozedwe ka Mawu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .158 Zowonjezera B: Zolemba Zowonjezera . . . . . . . . . . . . . . . . . . . . . .170 B.1 Opentrons Knowledge Hub. . . . . . . . . . . . . . . . . . . . . . . . . . . . 170 B.2 Python Protocol API zolemba. . . . . . . . . . . . . . . . . . . 170 B.3 Opentrons HTTP API reference. . . . . . . . . . . . . . . . . . . . . . . . 171 B.4 Zolemba za Wopanga . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171 Zowonjezera C: Mapulogalamu Otsegula Otsegula . . . . . . . . . . . . . . . . . . . . . . . . .172 C.1 Opentrons pa GitHub. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172 C.2 Opentrons monorepo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173 C.3 Nkhokwe zina . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175 Zowonjezera D: Thandizo ndi Mauthenga Othandizira . . . . . . . . . . . . . . .176 D.1 Zogulitsa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 D.2 Thandizo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 D.3 Zambiri zamabizinesi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177

Mawu Oyamba
Takulandilani ku bukhu lamalangizo la loboti yonyamula madzi ya Opentrons Flex. Bukuli limakuwongolerani pafupifupi chilichonse chomwe muyenera kudziwa kuti mukhazikitse ndikugwiritsa ntchito Flex, ndikuwunikira mitu yomwe ili yofunika kwambiri kwa ogwiritsa ntchito a Flex tsiku lililonse m'malo a labu.
Kapangidwe ka bukuli
Opentrons Flex ndi dongosolo lovuta, kotero pali njira zambiri zophunzirira zonse zomwe zingachite. Khalani omasuka kudumphira pamutu womwe ukunena za mutu uliwonse womwe mukufuna! Za exampKomabe, ngati muli ndi Flex yokhazikitsidwa kale mu labu yanu, mutha kudumpha kudutsa mutu wa Kukhazikitsa ndi Kusamutsa.
Ngati mukufuna njira yowongoleredwa, bukuli lapangidwa kuti muzitha kulitsatira kuyambira koyambira mpaka kumapeto.
Phunzirani za Flex. Zosiyanitsa za Flex zalembedwa mu Mutu 1: Mau oyamba. Chiyambichi chimaphatikizanso zambiri zokhudzana ndi chitetezo ndi malamulo.
Yambani ndi Flex. Ngati mukufuna kukhazikitsa Flex yanu, tsatirani malangizo atsatanetsatane mu Mutu 2: Kuyika ndi Kusamutsa. Kenako dziwani zigawo za Flex mu Chaputala 3: Kufotokozera Kwadongosolo.
Konzani malo anu. Kukonza sitimayo kumathandizira kugwiritsa ntchito zosiyanasiyana zasayansi pa Flex. Chaputala 4: Ma module akufotokoza zotumphukira za Opentrons zomwe mutha kuziyika kapena pamwamba pa sitimayo kuti mugwire ntchito zina zasayansi. Mutu 5: Labware ikufotokoza momwe mungagwiritsire ntchito zida zosungiramo zakumwa.
Pangani protocol. Kugwiritsa ntchito kwakukulu kwa Flex ndikuyendetsa njira zasayansi zokhazikika, zomwe zimadziwika kuti ma protocol. Chaputala 6: Kukula kwa Protocol kumapereka njira zingapo zopangira ma protocol okonzeka kapena kupanga nokha. Kuti mugwiritse ntchito protocol yanu, tsatirani malangizo omwe ali mu Chaputala 7: Mapulogalamu ndi Ntchito, yomwe ilinso ndi malangizo ogwirira ntchito zina ndikusintha makonda a loboti yanu.
Pitirizani kuyendetsa Flex. Tsatirani malangizo omwe ali mu Chaputala 8: Maintenance and Service kuti Flex yanu ikhale yaukhondo komanso ikuyenda bwino. Kapena lowani ku imodzi mwamautumiki a Opentrons omwe alembedwa pamenepo ndipo tiyeni tikusamalireni Flex.
Dziwani zambiri. Mukufunabe china? Onani zowonjezera. Zowonjezera A: Kalozera wa mawu amatanthauzira mawu okhudzana ndi Flex. Zowonjezera B: Zolemba Zowonjezera zimakulozerani kuzinthu zambiri zazinthu za Opentrons ndikulemba ma code kuti muwongolere Flex.

Mtengo wa OPENTRONS FLEX

MAWU OLANKHULIDWA
Zowonjezera C: Mapulogalamu Otsegula-Source amafotokoza momwe Opentrons imagwiritsidwira ntchito pa GitHub monga chothandizira kwa onse opanga ndi osapanga.
Zakumapeto D: Thandizo ndi Mauthenga Okhudzana ndi Mauthenga amalemba momwe mungalumikizire Opentrons ngati mukufuna thandizo loposa zomwe zolemba zathu zimapereka.
Zolemba ndi machenjezo
Mubukuli, mupeza zolemba ndi machenjezo opangidwa mwapadera. Zolemba zimapereka chidziwitso chothandiza chomwe sichingakhale chodziwikiratu mukamagwiritsa ntchito Flex. Samalani kwambiri machenjezo-amagwiritsidwa ntchito pokhapokha mutakhala pachiwopsezo chovulala, kuwonongeka kwa zida, kuwonongeka kapena kuwonongeka kwa s.ampzowononga kapena zopangira, kutayika kwa data, kapena kuvulaza kwina. Zolemba ndi machenjezo zikuwoneka motere:
Sample Dziwani: Izi ndi zomwe muyenera kudziwa, koma sizikuwopsa.
Sample Chenjezo: Ichi ndi chinthu chomwe muyenera kudziwa chifukwa pali chiopsezo chokhudzana nacho.

Mtengo wa OPENTRONS FLEX

MUTU 1
Mawu Oyamba

Mutuwu ukukudziwitsani za Opentrons Flex ecosystem, kuphatikiza kapangidwe kake kachitidwe ndi masanjidwe a malo ogwirira ntchito. Zimaphatikizanso zambiri zokhudzana ndi kutsata ndi chitetezo, zomwe muyenera kuyambiransoview musanakhazikitse loboti yanu ya Opentrons Flex. Kuti mumve zambiri pazambiri za Opentrons Flex, onani Mutu Wofotokozera Kachitidwe.

1.1 Takulandilani ku Opentrons Flex
Opentrons Flex ndi loboti yogwira zamadzimadzi yopangidwira kuti ikhale yodutsa kwambiri komanso mayendedwe ovuta. Roboti ya Flex ndiye maziko a ma modular system omwe amaphatikiza ma pipette, labware gripper, masitepe apamwamba, ma module apamtunda, ndi labware - zonse zomwe mungathe kuzisintha nokha. Flex idapangidwa ndi chotchinga cholumikizira kuti mutha kugwira nayo ntchito mwachindunji pa benchi ya labu, kapena mutha kuyiwongolera kuchokera pa labu yanu yonse ndi Opentrons App kapena ma API athu otsegula.
Malo ogwirira ntchito a Flex amabwera ndi zida zonse - loboti, zida, ndi labware - zomwe muyenera kuyamba kupanga ntchito wamba wamba. Kwa mapulogalamu ena, Opentrons Flex imayenda pa mapulogalamu otsegula ndi firmware, ndipo ndi reagent- ndi labware-agnostic, kukupatsani ulamuliro wa momwe mumapangira ndi kuyendetsa ma protocol anu.

Zatsopano mu Flex
Opentrons Flex ndi gawo la ma robot a Opentrons liquid handler. Ogwiritsa ntchito Opentrons Flex atha kudziwa bwino Opentrons OT-2, loboti yathu yamapaipi. Flex imadutsa mphamvu za OT-2 m'malo angapo ofunikira, kubweretsa kutulutsa kwapamwamba komanso nthawi yoyenda.

Kutulutsa kwa Pipette
Pipette ndi nsonga luso

Kufotokozera
Flex pipettes ali ndi 1, 8, kapena 96 njira. Pipette ya 96-channel imagwira ntchito pazitsime nthawi 12 nthawi imodzi monga pipette yaikulu ya OT-2.
Flex pipettes ali ndi ma voliyumu okulirapo (1 µL, 50 µL) ndipo amatha kugwira ntchito ndi maupangiri aliwonse a Opentrons Flex. Uku ndikuwongolera kuposa ma pipette a OT-5, omwe ali ndi mizere yaying'ono ndipo ayenera kugwiritsa ntchito malangizo okhala ndi kuchuluka kwa voliyumu yofananira.

Mtengo wa OPENTRONS FLEX

MUTU 1: MAU OYAMBA

Gripper Automated calibration Touchscreen Module caddies Deck slot imagwirizanitsa zinyalala zosunthika Kukula ndi kulemera kwake

Opentrons Flex Gripper imatenga ndikusuntha labware kuzungulira sitimayo, popanda kulowererapo kwa ogwiritsa ntchito. The gripper imathandizira kuti pakhale zovuta zogwirira ntchito mkati mwa protocol imodzi.
Kuyika kwapaipi kwa Flex pipettes ndi gripper ndizodziwikiratu. Dinani batani limodzi, ndipo chidacho chidzasunthira kumalo opangidwa molondola pa desiki kuti mudziwe komwe kuli, ndikusunga deta kuti mugwiritse ntchito muzolemba zanu.
Flex ili ndi mawonekedwe ake omwe amakulolani kuwongolera mwachindunji, kuphatikiza kugwiritsa ntchito Opentrons App. Gwiritsani ntchito zenera logwira kuti muyambe kuyendetsa ma protocol, fufuzani momwe ntchito ilili, ndikusintha makonda pa loboti.
Ma Flex modules amalowa mu makadi omwe amakhala pansi pa sitimayo. Ma Caddies amayika labware yanu pafupi ndi malo okwera ndikuloleza njira zolowera pansi. Ma Caddies amathandizira masanjidwe ochulukirapo a module ndi labware pa sitimayo.
Malo otsetsereka pa Flex amawerengedwa ndi makina ogwirizanitsa (A1D4) omwe ali ofanana ndi momwe zitsime zimawerengedwera pa labware.
Bira la zinyalala limatha kupita m'malo angapo pa Flex. Malo osakhazikika (kagawo A3) ndi malo omwe akulimbikitsidwa. Mukhozanso kugwiritsa ntchito chophatikizira kutaya zinyalala mu chute ya zinyalala zomwe mungasankhe.
Flex ndi yayikulupo komanso yolemera kwambiri kuposa OT-2. Ntchito zoyika pa Flex zimafuna kuthandizidwa ndi wothandizana nawo labu.

Kuyerekeza mwatsatanetsatane kwaukadaulo wamaloboti kulipo pa Opentrons webmalo.
Maloboti onse a Flex ndi OT-2 amayendetsa pulogalamu yathu yotsegula, ndipo Opentrons App imatha kuwongolera mitundu yonse ya maloboti nthawi imodzi. Ngakhale ma protocol a OT-2 sangathe kuyendetsedwa mwachindunji pa Flex, ndikosavuta kuwasintha (onani gawo la OT-2 Protocols la mutu wa Protocol Development kuti mumve zambiri).

Flex workstations
Malo ogwirira ntchito a Opentrons Flex akuphatikiza loboti ya Flex, zowonjezera, ma pipette ndi gripper, ma module apamtunda, ndi ma labware ofunikira kuti agwiritse ntchito pulogalamu inayake. Zida zonse zogwirira ntchito ndi modular. Ngati mukufuna kusintha mapulogalamu, mutha kuwonjezera kapena kusinthana ndi zida zina za Flex ndi zogwiritsira ntchito.

Mtengo wa OPENTRONS FLEX

MUTU 1: MAU OYAMBA
NGS NTCHITO
Opentrons Flex NGS Workstation imagwiritsa ntchito laibulale ya NGS prep. Itha kusinthiratu kusanja kachitidwe ka ntchito pogwiritsa ntchito makina aliwonse otsogola, kuphatikiza kugawikana- ndi tagkukonzekereratu kwa library.
Kuphatikiza pa loboti ya Flex, NGS Workstation ikuphatikiza:
Gripper Kusankha kwa kasinthidwe ka pipette
Mapaipi awiri a 8-Channel (1 µL ndi 50 µL) 5-Channel Pipette (1000 µL) Waste Chute Magnetic Block Temperature Module Thermocycler Module Labware kit yokhala ndi nsonga zosefera, machubu a microcentrifuge, zosungira, ndi mbale za PCR.
PCR WORKSTATION
Opentrons Flex PCR Workstation imagwiritsa ntchito kukhazikitsidwa kwa PCR ndikuyenda kwa thermocycling mpaka 96 s.amples. Iwo akhoza aliquot chilled reagents ndi samplekani mu mbale ya PCR ya 96-chitsime. Pogwiritsa ntchito makina a Thermocycler Module, gwiritsani ntchito chophatikizira kuti mukweze mbale mu Thermocycler, ndiyeno yendetsani pulogalamu ya PCR yomwe mwasankha.
Kuphatikiza pa loboti ya Flex, PCR Workstation imaphatikizapo:
Gripper Kusankha kwa kasinthidwe ka pipette
1-Channel Pipette (1 µL) ndi 50-Channel Pipette (8 µL) 1-Channel Pipette (50 µL) Waste Chute Temperature Module Labware kit yokhala ndi nsonga zosefera, machubu a microcentrifuge, malo osungira, ndi mbale za PCR

Mtengo wa OPENTRONS FLEX

MUTU 1: MAU OYAMBA
NUCLEIC ACID EXTRACTION WORKSTATION
Opentrons Flex Nucleic Acid Extraction Workstation imagwiritsa ntchito kudzipatula kwa DNA/RNA ndikuyeretsa. Imagwiritsa ntchito Magnetic Block kulekanitsa mikanda ya maginito, ndi Heater-Shaker kwa s.ample lysis ndi kubwezeretsanso mikanda ya maginito.
Kuphatikiza pa loboti ya Flex, Nucleic Acid Extraction Workstation imaphatikizapo:
Gripper Kusankha kwa kasinthidwe ka pipette
1-Channel Pipette (5 µL) ndi 1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL) Waste Chute Magnetic Block Heater-Shaker Module Labware kit yokhala ndi nsonga zosefera, zosungira, mbale za PCR, ndi mbale zakuya za PCR.
MAGNETIC BEAD PROTEIN PURIFICATION WORKSTATION
Opentrons Flex Magnetic Bead Protein Purification Workstation imapanga makina ang'onoang'ono oyeretsera mapuloteni ndi ma proteinomics.ampndikukonzekera mpaka 96 samples. Imagwirizana ndi ma reagents ambiri otchuka a maginito.
Kuphatikiza pa loboti ya Flex, Protein Purification Workstation imaphatikizapo:
Gripper Kusankha kwa kasinthidwe ka pipette
1-Channel Pipette (5 µL) ndi 1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL) Waste Chute Magnetic Block Heater-Shaker Module Labware kit yokhala ndi nsonga zosefera, zosungira, mbale za PCR, ndi mbale zakuya za PCR.
FLEX PREP WORKSTATION
Opentrons Flex Prep Workstation imagwiritsa ntchito ma pipetting osavuta. Konzani malo ogwirira ntchito ndi 1-Channel ndi 8-Channel Pipettes kuti mugwire ntchito monga sampndi transfer, sample kubwereza, ndi

Mtengo wa OPENTRONS FLEX

MUTU 1: MAU OYAMBA
reagent aliquoting. Konzani malo ogwirira ntchito ndi 96-Channel Pipette kuti mugwiritse ntchito aliquoting yamphamvu kwambiri komanso mbale stampndi.
Kuphatikiza pa loboti ya Flex, Flex Prep Workstation imaphatikizapo:
Kusankha kwa masinthidwe a pipette: 1-Channel Pipette (5 µL) ndi 1000-Channel Pipette (8 µL) 5-Channel Pipette (1000 µL)
Labware kit yokhala ndi nsonga zosefera, machubu a microcentrifuge, ndi zosungira
PLAMID PREP WORKSTATION
Opentrons Flex Plasmid Prep Workstation imagwiritsa ntchito maginito opangidwa ndi mikanda ya plasmid ndikuyenda kwa ntchito yoyeretsa. Malo ogwirira ntchitowa ali ndi ma pipette apamwamba kwambiri, Heater-Shaker Module, ndi Magnetic Block kuti agwirizane ndi chemistry yopangidwa ndi mikanda.
Kuphatikiza pa loboti ya Flex, Plasmid Prep Workstation imaphatikizapo:
Gripper 1-Channel Pipette (5 µL) ndi 1000-Channel Pipette (8 µL) Waste Chute Magnetic Block Heater-Shaker Module Labware kit yokhala ndi zosefera, machubu a microcentrifuge, malo osungira, mbale za PCR, ndi mbale zakuya zakuya.
SYNBIO NTCHITO
Opentrons Flex SynBio Workstation imapanga makina osiyanasiyana opangira biology monga DNA synthesis ndi cloning. Zimagwiritsa ntchito Magnetic Block ndi Temperature Module kuti zithandizire chemistry yozikidwa ndi mikanda. Onjezani Thermocycler Module kuti mupange ma incubations otentha ndi ampzikomo.
Kuphatikiza pa loboti ya Flex, SynBio Workstation ikuphatikiza:
Gripper 1-Channel Pipette (5 µL) ndi 1000-Channel Pipette (8 µL) Magnetic Block Temperature Module

Mtengo wa OPENTRONS FLEX

MUTU 1: MAU OYAMBA

Labware kit yokhala ndi malangizo anthawi zonse komanso zosefera, machubu a microcentrifuge, malo osungira, mbale za PCR, ndi mbale zakuya zakuya.

1.2 Zambiri zachitetezo
Loboti ya Opentrons Flex yonyamula madzi yapangidwa kuti igwire ntchito bwino. Onani zomwe zafotokozedwa mugawoli kuti muwonetsetse kugwiritsa ntchito Flex yanu motetezeka. Malangizowa akukhudza kugwiritsa ntchito kotetezeka kwa zolowetsa ndi zotulutsa zolumikizana ndi zinthu, kuphatikiza mphamvu ndi kulumikizana kwa data, komanso zilembo zochenjeza zopezeka pa roboti ya Flex ndi zida zofananira. Kugwiritsa ntchito chipangizocho m'njira zina osati zomwe zafotokozedwa m'bukuli kukhoza kuyika wogwiritsa ntchito ndi zida pachiwopsezo.

Zizindikiro zachitetezo
Zolemba zosiyanasiyana pa Flex ndi bukuli zimakuchenjezani za komwe mungavulale kapena kuvulala.

Chizindikiro

Kufotokozera
Chenjezo: Imachenjeza ogwiritsa ntchito zinthu zomwe zitha kukhala zoopsa. Zochita zomwe zingabweretse kuvulala kapena imfa.
Chenjezo: Imachenjeza ogwiritsa ntchito Kuwonongeka kwa zida. Deta yotayika kapena yoyipa. Kusokoneza kosabweza kwa opareshoni yomwe ikuchitika.
Kugwedezeka kwamagetsi: Kumazindikiritsa zida zomwe zitha kukhala pachiwopsezo cha kugwedezeka kwamagetsi ngati chida sichinasamalidwe bwino.

Malo otentha: Amazindikiritsa zida zomwe zingawononge munthu chifukwa cha kutentha kwambiri / kutentha ngati chidacho sichikugwiritsidwa ntchito bwino.

Pinch point: Imazindikiritsa zida zomwe zitha kuvulaza munthu akamayenda.

Mtengo wa OPENTRONS FLEX

MUTU 1: MAU OYAMBA

Mupeza zolemba zotsatirazi pa Flex:
Malebulo a zinthu zaluntha Malebulo ovomerezeka (monga ETL) Malebulo owopsa a magetsi Malebulo ochenjeza Malebulo azinthu Zolemba Pinch point High voltage amalemba zilembo za Power rating

Machenjezo okhudza chitetezo chamagetsi
Nthawi zonse samalani machenjezo awa okhudzana ndi chitetezo chamagetsi:

Chizindikiro

Kufotokozera
Lumikizani loboti pagawo lokhazikika, la Gulu loyamba. Onani gawo la Power Connection mumutu wa Kufotokozera Kwadongosolo.

Osalumikiza (pulagi), chotsani (chombola), kapena gwiritsani ntchito zingwe zamagetsi za AC ngati: Chingwe chaphwanyidwa kapena chawonongeka. Zingwe zomata, zingwe, kapena zotengera zina zimaduka kapena kuwonongeka.
Kugwiritsa ntchito zingwe zamagetsi zowonongeka kungayambitse ngozi yamagetsi yomwe imabweretsa kuvulala kwambiri kapena kuwonongeka kwa loboti.
Osalowa m'malo mwa chingwe chamagetsi cha AC pokhapokha panjira ya Opentrons Support.

Kuti mumve zambiri pazofunikira zamagetsi, onani gawo la Power Consumption la mutu wa Installation and Relocation.

Mtengo wa OPENTRONS FLEX

MUTU 1: MAU OYAMBA

Machenjezo owonjezera otetezera
Nthawi zonse sungani machenjezo owonjezera awa:

Chizindikiro

Kufotokozera
Opentrons Flex sinavomerezedwe kuti igwiritsidwe ntchito ndi zakumwa zophulika kapena zoyaka. Osalowetsa mbale, machubu, kapena mbale zokhala ndi zamadzimadzi zophulika kapena zoyaka mu robot kapena kugwiritsa ntchito chidacho ndi zamadzimadzi zophulika kapena zoyaka mumpanda.
Gwiritsani ntchito machitidwe abwino a labotale ndipo tsatirani njira za wopanga pogwira ntchito ndi mankhwala. Opentrons alibe udindo kapena wolakwa chifukwa cha, kapena chifukwa cha, kugwiritsa ntchito mankhwala owopsa.

Flex imalemera 88.5 kg (195 lbs). Chifukwa cha zimenezi, pamafunika anthu awiri kuti anyamule ndi kulisuntha bwinobwino. Onani gawo la Relocation mumutu wa Kukhazikitsa ndi Kusamutsa.

Flex iyenera kuyikidwa pamtunda womwe ungathe kuthandizira kulemera kwake kwa 88.5 kg (195 lbs) ndi malo okwanira kuti agwirizane ndi loboti kuphatikizapo mtunda wake wochepa (20 cm / 8 mkati). Onani gawo la Safety and Operating Requirements pamutu wa Kukhazikitsa ndi Kusamutsa.
Flex imatha kutulutsa ma vibrate ikugwira ntchito. Ikani loboti pamalo olimba, osalala, osamva madzi okhala ndi zolumikizira zopingasa kapena zomata. Onani gawo la Safety and Operating Requirements pamutu wa Kukhazikitsa ndi Kusamutsa.

Chenjezo lachitetezo
Kuti muteteze Flex kuti isawonongeke, tsatirani izi:

Mtengo wa OPENTRONS FLEX

MUTU 1: MAU OYAMBA

Chizindikiro

Kufotokozera
Gwiritsani ntchito labware yomwe imagwirizana ndi ANSI/SLAS kapena yovomerezedwa ndi Opentrons. Onani mutu wa Labware.
Sungani zinthu zowononga, zowononga, kapena zinthu zina zowononga kutali ndi loboti.

Chitetezo chachilengedwe
Tetezani zitsanzo ndi zopangira zokhala ndi zinthu zotengedwa kuchokera kwa anthu ngati zitha kupatsirana. Opentrons amalimbikitsa kugwiritsa ntchito njira zotetezeka za labotale monga zafotokozedwera mu Biosafety in Microbiological and Biomedical Laboratories (BMBL) 6th Edition.
Nthawi zonse, Flex sipanga ma aerosol owoneka kuchokera kumadzimadzi. Komabe, mumikhalidwe ina, ndizotheka kupanga ma aerosols kuchokera kuzinthu zamadzimadzi. Mukamagwira ntchito ndi biosafety level 2 kapena zamadzimadzi zokulirapo, ganizirani kusamala kuti musamakhale ndi mpweya wa aerosol, molingana ndi mabungwe oyang'anira kwanuko. Kuti muchepetse chiwopsezo chopezeka ndi aerosol kuchokera ku loboti, onetsetsani kuti:
Chitani zokonza monga zafotokozedwera mu mutu wa Maintenance and Service. Konzani bwino ndikuteteza zida zonse, ma pipettes, ma modules, ndi labware. Gwiritsani ntchito njira yoyenera yopangira mapaipi kuti muchepetse ma aerosols.
Utsi wapoizoni
Ngati mukugwira ntchito ndi zosungunulira zosasunthika kapena zinthu zapoizoni, gwiritsani ntchito mpweya wabwino wa mu labotale kuti muchotse nthunzi iliyonse yomwe ingapangidwe.
Zamadzimadzi zoyaka moto
Flex sinayesedwe kuti igwiritsidwe ntchito ndi zakumwa zoyaka ndipo sayenera kugwiritsidwa ntchito ndi zakumwa zoyaka.

Mtengo wa OPENTRONS FLEX

MUTU 1: MAU OYAMBA

1.3 Kutsata malamulo
Opentrons Flex imagwirizana ndi zofunikira zonse zachitetezo chotsatirachi ndi miyezo yamagetsi yamagetsi.

Chitetezo
Lamulo ID IEC/UL/CSA 61010-1 IEC/UL/CSA 61010-2-051

Mutu
Zofunikira Zachitetezo Pazida Zamagetsi Poyezera, Kuwongolera, ndi Kugwiritsa Ntchito Laborator Gawo 1: Zofunikira Zonse
Zofunikira makamaka pazida za labotale zosakaniza ndi kugwedeza

Kugwirizana kwa electromagnetic

Chidziwitso chalamulo EN/BSI 61326-1
FCC 47 CFR Gawo 15 Gawo B Kalasi A IC ICES-003

Mutu
Zida Zamagetsi Zoyezera, Kuwongolera, ndi Kugwiritsa Ntchito Laboratory Zofunikira za EMC Gawo 1: Zofunikira Zazikulu Zofunikira Ma Radiators Osafuna
Spectrum Management and Telecommunications InterferenceCausing Equipment Standard Information Technology Equipment (Kuphatikiza Digital Apparatus)

Machenjezo ndi zolemba za FCC
Chenjezo: Kusintha kapena kusinthidwa kwa gawoli lomwe silinavomerezedwe mwachindunji ndi Opentrons kungathe kulepheretsa wogwiritsa ntchito kugwiritsa ntchito zidazo.
Chipangizochi chikugwirizana ndi Gawo 15 la malamulo a FCC. Kugwira ntchito kumadalira izi:

Mtengo wa OPENTRONS FLEX

MUTU 1: MAU OYAMBA
Chipangizochi sichingabweretse zosokoneza. Chipangizochi chiyenera kuvomereza kusokonezedwa kulikonse komwe kulandidwa, kuphatikiza kusokoneza komwe kungayambitse mosayenera
ntchito.
Zindikirani: Zidazi zayesedwa ndipo zapezeka kuti zikugwirizana ndi malire a chipangizo cha digito cha Gulu A, motsatira Gawo 15 la malamulo a FCC. Malirewa amapangidwa kuti apereke chitetezo chokwanira ku zosokoneza zovulaza pamene zida zikugwiritsidwa ntchito kumalo amalonda. Zipangizozi zimapanga, zimagwiritsa ntchito, komanso zimatha kuwunikira mphamvu zamawayilesi ndipo, ngati sizinayikidwe ndikugwiritsidwa ntchito motsatira buku la malangizo, zitha kusokoneza njira zolumikizirana ndi wailesi. Kugwiritsiridwa ntchito kwa chipangizochi m’malo okhala anthu kungadzetse kusokoneza koopsa kotero kuti wogwiritsa ntchitoyo adzafunika kukonza zosokonezazo ndi ndalama zake.
Canada ISED kutsatira
Canada ICES-003(A) / NMB-003(A)
Izi zimakwaniritsa zofunikira za Innovation, Science and Economic Development Canada.
Le présent produit est conforme aux spécifications techniques d'Innovation, Sciences et Developpement économique Canada.
Chenjezo la chilengedwe
Chenjezo: Khansa ndi Kuvulaza Ubereki www.P65Warnings.ca.gov
Wi-Fi precertification
Module ya Wi-Fi ndi yovomerezeka kuti igwiritsidwe ntchito m'madera ambiri:
United States (FCC): FCC Identifier UAY-W8997-M1216 European Economic Area (CE): Palibe chizindikiritso chapagulu (kudzidziwitsa) Canada (IC): Hardware Version Identification Number W8997-M1216 Japan (TELEC): Nambala yovomerezeka 020-170034 India (WPC): Nambala yolembetsa ETA-SD-20191005525 (kudzilengeza)

Mtengo wa OPENTRONS FLEX

MUTU 2
Kukhazikitsa ndi kusamutsa
Mutuwu ukufotokoza momwe mungakonzekerere labu yanu ya Opentrons Flex, momwe mungakhazikitsire loboti, komanso momwe mungayendetsere ngati kuli kofunikira. Musanatumize Flex yanu, onetsetsani kuti labu yanu kapena malo anu akukwaniritsa zonse zomwe zili mu gawo la Safety and Operating Requirements. Ikafika nthawi yoti muyambe kuyendetsa Flex yanu, tsatirani malangizo atsatanetsatane mugawo la Unboxing, First Run, ndi Instrument Installation and Calibration, kapena gwiritsani ntchito Opentrons Onsite Support Set Up service. Ndipo ngati mungafunike kusuntha Flex yanu kupita kumalo atsopano, pafupi kapena kutali, tsatirani njira zomwe zili mu gawo la Relocation.
2.1 Zofunikira pachitetezo ndi magwiridwe antchito
Kumene mungayike Opentrons Flex
Space ndi chinthu chamtengo wapatali pafupifupi labu iliyonse. Flex yanu ifunika zina-koma osati zochuluka, chifukwa idapangidwa kuti igwirizane ndi theka la benchi wamba wamba. Onetsetsani kuti muli ndi malo omwe akugwirizana ndi izi.
Pamwamba pa benchi: Pamalo osasunthika, olimba, osasunthika, osagwira madzi. Matebulo kapena mabenchi okhala ndi mawilo (ngakhale mawilo okhoma) savomerezedwa. Flex imayenda mwachangu ndipo imakhala ndi misa yambiri, yomwe imatha kugwedezeka kapena kusalinganiza magome opepuka kapena osunthika.
Kulemera kwake: Loboti yokhayo imalemera 88.5 kg (195 lb) ndipo iyenera kukwezedwa ndi anthu awiri ogwira ntchito limodzi. Ikani loboti pamalo omwe amatha kunyamula kulemera kwake kuphatikiza kulemera kwa ma module, labware, zakumwa, kapena zida zina za labu zomwe mungagwiritse ntchito polemba.
Malo ogwiritsira ntchito: Miyeso yoyambira ya robot ndi 87 cm W x 69 cm D x 84 cm H (pafupifupi 34 ″ x 27 ″ x 33 ″). Flex imafunikira 20 cm (8 ″) ya chilolezo cham'mbali ndi chakumbuyo cha zingwe, zolumikizira za USB, ndikuchotsa utsi kuchokera kuma module omwe amatentha komanso ozizira.
Chenjezo: Osayika mbali kapena kumbuyo kwa Flex pakhoma.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA

84 cm 33 "

87 cm 34 "
Opentrons Flex miyeso yoyambira.

69 cm 27 "

20 cm 8 "

Pamwamba view ya Opentrons Flex, yowonetsa mbali yocheperako ndi kumbuyo.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA

Kugwiritsa ntchito mphamvu
Opentrons Flex iyenera kulumikizidwa ku khoma lomwe lili pafupi ndi benchi yomwe mumayiyika. Ingolumikizani Flex ku mabwalo omwe amatha kutengera mphamvu yake yayikulu:
Mphamvu zolowetsa: 36 VDC, 6.1 A Kugwiritsa ntchito mopanda ntchito: 30 W Kugwiritsa ntchito nthawi zonse: 40 W (panthawi yoyendetsa protocol) Kugwiritsa ntchito kwambiri: Pafupifupi 50 W
Kugwiritsa ntchito mphamvu kwenikweni kumadalira:
Kuchuluka ndi mtundu wa kayendedwe kachitidwe pa protocol. Nthawi yochuluka yomwe robot imakhala yopanda ntchito. Mkhalidwe wa magetsi pa robot. Ndi zida zingati zomwe zimalumikizidwa.
Kumbukirani kuwerengera zida zina zamagetsi zomwe zimawononga mphamvu pamagawo omwewo, kuphatikiza ma module a Flex okhala ndi mphamvu zawo. Za exampLero, Thermocycler Module ili ndi mphamvu zambiri (630 W) zomwe zimakhala zazikulu kwambiri kuposa loboti ya Flex yomwe. Ngati kuli kofunikira, funsani woyang'anira malo anu kuti atsimikizire kuti akukwaniritsa zofunikira zamagetsi a chipangizo chanu.

Mikhalidwe ya chilengedwe
Mikhalidwe ya chilengedwe yovomerezeka kuti igwiritsidwe ntchito, yovomerezeka, ndi kusungidwa imasiyana:

Analimbikitsa ntchito dongosolo

Zovomerezeka pakugwiritsa ntchito dongosolo

Kutentha kozungulira +20 mpaka +25 °C

+2 mpaka +40 °C

Chinyezi chachibale Kutalika

40%, osafupikitsa
Pafupifupi 500 m pamwamba pa nyanja

30%, osasunthika (pansi pa 80 °C)
Kufikira 2000 m pamwamba pa nyanja

Kusungirako ndi mayendedwe
-10 mpaka +60 °C
10%, osasunthika (pansi pa 85 °C)
Kufikira 2000 m pamwamba pa nyanja

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
Opentrons yatsimikizira magwiridwe antchito a Opentrons Flex m'mikhalidwe yomwe ikulimbikitsidwa kuti igwiritsidwe ntchito pamakina, ndipo kugwira ntchito m'mikhalidwe imeneyi kuyenera kupereka zotsatira zabwino. Flex ndiyotetezeka kugwiritsidwa ntchito m'malo ovomerezeka pamakina, koma zotsatira zimatha kusiyana. Osayatsa kapena kugwiritsa ntchito Flex ngati zili kunja kwa malirewo. Zosungirako ndi zoyendetsa zimangogwira ntchito pamene loboti imachotsedwa kwathunthu ku mphamvu ndi zipangizo zina.
2.2 Unboxing
Zabwino zonse! Opentrons Flex yanu yafika ndipo mwakonzekera malo mu labu yanu. Tiyeni titsegule crate ya chilombocho, tichotse lobotiyo, ndikukonzekera kuti igwire ntchito. Zomwe zili mugawoli zimapereka mndandanda wa magawo ndi malangizo omwe amakuyendetsani njira zomwe zimafunikira kuti Flex ikhale yosakanizidwa, ikhazikitsidwe, ndikukonzekera kugwiritsidwa ntchito. Tagawa njira yokhazikitsira m'magawo atatu:
Gawo 1 limakhudza kupatulira crate. Gawo 2 limakwirira kuchotsa Flex kuchokera mu crate ndikuyisunthira kumalo omaliza a msonkhano. Gawo 3 limakhudza msonkhano womaliza ndikuyika mphamvu pa loboti kwa nthawi yoyamba.
Khama ndi nthawi yofunika
Mufuna kupempha mnzanu wa labu kuti akuthandizeni ndi unboxing, kukweza, kusuntha, ndi kusonkhana. Muyenera kupanga bajeti pafupifupi mphindi 30 mpaka ola kuti muchite izi.
Zindikirani: Flex imafuna anthu awiri kuti ayikweze bwino. Komanso, kukweza ndi kunyamula Flex ndi manja ake ndiyo njira yabwino yosunthira robot.
Kreti ndi zonyamula katundu
Kutsegula Flex kumakupatsani loboti yodabwitsa, koma mumasiyidwanso ndi mapanelo akulu akulu akulu limodzi ndi zida zotumizira zosiyanasiyana komanso zopalasa. Ngakhale mutataya zinthuzi, tikukulimbikitsani kuti muzisunga zinthu zimenezi ngati malo osungira alipo. Zopakapaka zimatha kugwiritsidwanso ntchito, zomwe zimathandiza kukonzekera Flex yanu kuti itumizidwe ngati mukufuna kuitumiza kwina (mwachitsanzo, kumsonkhano kapena malo atsopano) m'tsogolomu.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
Zinthu zopangidwa
Sitima za Flex zomwe zili ndi zigawo zomwe zili pansipa. Ma Pipettes, gripper, ndi ma module amabwera m'matumba osiyana kuchokera ku Flex crate yayikulu, ngakhale mutawagula limodzi ngati malo ogwirira ntchito.

(1) Opentrons Flex loboti

(1) Chingwe cha USB

(1) Chingwe chamagetsi

(1) Chingwe cha Ethernet

(5) L-makiyi (12 mm hex, 1.5 mm hex, 2.5 mm hex, 3 mm hex),
T10 Torx)

(1) Emergency Stop Pendant

(1) Deck slot yokhala ndi ma labware clips

(4) Makanema osungira labware

(1) Pipette calibration probe

(4) Kunyamula zogwirira ndi zipewa

(1) Mawindo apamwamba kwambiri

(4) Mazenera am'mbali

(1) 2.5 mm hex screwdriver

(1) 19 mm wrench

(16 + zotsalira) Zomangira mawindo (M4x8 mm mutu wathyathyathya)
26

(10) Zomangira zapadeck (M4x10 mm socket mutu)

(12) Zomangira zapadeck (M3x6 mm socket mutu)

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
Gawo 1: Chotsani crate
Opentrons imatumiza Flex yanu mu crate yolimba ya plywood. Klasi yotumizira imagwiritsa ntchito mbedza ndi latch clamps kuteteza pamwamba, mbali, ndi pansi mapanelo palimodzi. Kugwiritsa ntchito zingwe, m'malo mwa misomali kapena zomangira, kumatanthauza kuti simudzasowa khwangwala (kapena mphamvu zambiri) kuti mutsegule crate, ndipo mutha kuyiphatikizanso pambuyo pake, ngati pakufunika.
Zindikirani: M'mphepete mwa ma crate amatha kukhala oyipa panthawi yotumiza. Mungafune kugwiritsa ntchito magolovesi ogwira ntchito kuti muteteze manja anu ku zidutswa zamatabwa.
Kuti mutulutse zingwe, tembenuzirani tabu ya latch mmwamba ndikuitembenuzira kumanzere (motsatira koloko). Izi zimasuntha clamp tulutsani m'mabulaketi ake osungira. Kenako mutha kutembenuza mkono wa latch kuchoka pa crate.
1 Tsegulani zingwe zisanu ndi zitatu zomwe zikugwira pamwamba m'mbali.

2 Chotsani gulu lapamwamba mutatulutsa zingwe.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUSINTHA NDI KUSINTHA 3 Dulani thumba la buluu lotumizira, chotsani zinthu izi pa padding, ndi kuziyika pambali:
User Kit Power, Efaneti, ndi zingwe za USB Emergency Stop Pendant
4 Chotsani chidutswa chapamwamba cha thovu kuti muwonetse mazenera. Padding amateteza mbali ndi pamwamba mapanelo.
5 Chotsani mazenera ndi kuwaika pambali. Muziphatikiza pambuyo pake.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
6 Tsegulani zingwe zotsalira 16 zomwe zimagwira mapanelo am'mbali wina ndi mnzake komanso m'munsi mwa crate. 7 Chotsani mapanelo am’mbali ndi kuwaika pambali.
Gawo 2: Tulutsani Flex
Mukamaliza masitepe omwe ali mu Gawo 1, tsopano muyenera kuwona loboti yomwe ili m'chikwama chodzitchinjiriza ndikumangirizidwa ku zida zomangira zitsulo zalalanje. Chikwamacho chimatseka loboti ndikuyiteteza ku chilengedwe chakunja. Mabulaketi achitsulo amateteza loboti pansi pa crate. Mafelemu awiri otumizira amathandizira loboti, kugawa kulemera kwake mofanana, ndikuisunga yolimba kuti isasunthike potumiza. Pitirizani kumasula Flex ndikuyichotsa pa crate.
8 Pogwiritsa ntchito wrench ya 19 mm kuchokera pa User Kit, masulani mabokosi kuchokera pansi pa crate. Mutha kutaya mabulaketi, kapena kuwasunga kuti mudzagwiritse ntchito mtsogolo.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUSINTHA NDI KUSINTHA 9 Kokani kapena pindani chikwama chotumizira mpaka pansi kuti muwonetse loboti yonse.
10 Mothandizidwa ndi mnzako wa labu, gwirani zogwira m'manja mumafelemu otumizira malalanje mbali zonse za maziko a loboti, kwezani Flex kuchoka pa crate, ndikuyiyika pansi. Sungani kapena kutaya maziko a crate ndi chimango chotumizira.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUSINTHA NDI KUSINTHA 11 Pogwiritsa ntchito kiyi ya 12 mm hex L kuchokera ku User Kit, chotsani mabawuti anayi omwe ali ndi mafelemu otumizira.
Flex. Sungani kapena kutaya mafelemu ndi mabawuti.
12 Chotsani zogwirira ntchito zinayi za aluminiyamu pa User Kit. Sungani zogwirira ntchito m'malo omwewo omwe anali ndi mabawuti otumizira 12 mm.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
13 Mothandizidwa ndi mnzanu wa mu labu, kwezani Flex ndi zonyamula zake ndikuyisunthira pa benchi yogwirira ntchito kuti musonkhene komaliza.

Gawo 3: Msonkhano womaliza ndikuyatsa mphamvu
Mukasuntha Flex kumalo ogwirira ntchito kwakanthawi, kapena kwawo kwamuyaya, ndi nthawi yoti muyike zomaliza pa robot yanu yatsopano.
14 Ngati mwasuntha loboti kumalo ake omaliza, ogwirira ntchito, chotsani zogwirira ntchito ndikuzisintha ndi zipewa zomaliza. Zipewazo zimatseka zogwirira ntchito mu chimango ndikupangitsa kuti loboti ikhale yoyera. Bweretsani zogwirira ku User Kit kuti musunge.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
15 Bweretsani mapepala apamwamba ndi am'mbali kuchokera ku thovu lonyamulira lomwe mwayika pambali mutachotsa pamwamba pa crate.
16 Gwirizanitsani mazenera ku Flex potsatira zomwe zalembedwa pafilimu yoteteza kutsogolo. Ndiye chotsani zoteteza filimu.
17 Pogwiritsa ntchito zomangira zazenera zopindika ndi screwdriver ya 2.5 mm kuchokera pa User Kit, ikani mazenera ku Flex. Onetsetsani kuti mabowo opindika (oboola ngati V) pazenera akuyang'ana kunja (kwa inu). Izi zimathandiza kuti zomangira zigwirizane ndi pamwamba pa zenera.

Chenjezo: Kuwongolera mapanelo molakwika kungayambitse kuwonongeka. Kuchuluka kwa screw torque kumatha kuphwanya mapanelo. Limbani zomangira pamanja mpaka mazenera akhale otetezeka. Uku si kuyesa mphamvu.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA 18 Pogwiritsa ntchito screwdriver ya 2.5 mm kuchokera ku User Kit, chotsani zomangira zokhoma pa gantry. Izi
zomangira zimalepheretsa gantry kusuntha mukamayenda. Zomangira zotsekera za gantry zili: Kumanzere njanji pafupi ndi kutsogolo kwa loboti. Pansi pa mkono woyima wa gantry. Kumanja njanji pafupi kutsogolo kwa loboti mu bulaketi lalanje. Pali zomangira ziwiri apa.
Gantry imayenda mosavuta ndi dzanja mutachotsa zomangira zonse zotumizira. 19 Dulani ndi kuchotsa mphira ziwiri zomwe zimasunga nkhokwe ya zinyalala panthawi yotumiza.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
20 Gwirizanitsani chingwe chamagetsi ku Flex ndikuchilumikiza pakhoma. Onetsetsani kuti malo a sitimayo mulibe zopinga. Yendetsani chosinthira mphamvu chakumbuyo kumanzere kwa loboti. Akayatsidwa, gantry imasunthira kumalo ake ndipo chojambula chojambula chikuwonetsa malangizo owonjezera okonzekera.

Tsopano popeza Flex yanu yatuluka m'bokosi ndipo mwakonzeka kupita, pitilizani kugawo loyamba la Run pansipa.
2.3 Kuthamanga koyamba
Pangani zokhazikitsira zoyambira pa touchscreen musanalumikizane ndi zida zina zilizonse ku Flex yanu. Robotiyo ikutsogolerani pakulumikizana ndi netiweki yanu ya labu, kusinthira ku mapulogalamu aposachedwa, ndikusintha makonda anu Flex powapatsa dzina.
Yatsani
Mukatsegula Flex, chizindikiro cha Opentrons chidzawonekera pazenera. Pambuyo mphindi zochepa, iwonetsa chophimba cha "Welcome to your Opentrons Flex".
Pulogalamu yolandila ya Opentrons Flex. Muyenera kuwona chophimba ichi mukayamba Flex yanu koyamba.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
Lumikizani ku netiweki kapena kompyuta
Tsatirani zomwe zili pa touchscreen kuti loboti yanu ilumikizidwe kuti iwonetse zosintha zamapulogalamu ndikulandila protocol files. Pali njira zitatu zolumikizira: Wi-Fi, Efaneti, ndi USB.

Zosankha zolumikizirana ndi netiweki. Muyenera kukhala ndi intaneti kuti mukhazikitse Flex. Wi-Fi: Gwiritsani ntchito chophimba cholumikizira kuti mulumikizane ndi ma netiweki a Wi-Fi omwe ali otetezedwa ndi WPA2 Personal authentication (mamanetiweki ambiri omwe amangofunika mawu achinsinsi kuti alowe nawo amagwera m'gululi).
Zindikirani: Flex sigwirizana ndi ma portal ogwidwa (ma network omwe alibe mawu achinsinsi koma a webtsamba kutsimikizira ogwiritsa ntchito pambuyo polumikizana).
Mutha kulumikizananso ndi netiweki yotseguka ya Wi-Fi, koma izi sizovomerezeka.
Chenjezo: Kulumikizana ndi netiweki ya Wi-Fi yotseguka kudzalola aliyense wamitundu yosiyanasiyana ya netiweki kuwongolera loboti yanu ya Opentrons Flex popanda kutsimikizika.

Ngati mukufuna kulumikiza netiweki ya Wi-Fi yomwe imagwiritsa ntchito kutsimikizika kwabizinesi (kuphatikiza "eduroam" ndi maukonde amaphunziro ofanana omwe amafunikira dzina lolowera ndi mawu achinsinsi), choyamba lumikizani ku Opentrons App ndi Ethernet kapena USB kuti mumalize kuyika koyambirira. Kenako gwirizanitsani ndi netiweki ya Wi-Fi pamakina ochezera a Flex yanu. Kuti mupeze zokonda pamanetiweki:

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
1. Dinani Zipangizo kumanzere kwa Opentrons App. 2. Dinani menyu ya madontho atatu () ya Flex yanu ndikusankha Zikhazikiko za Robot. 3. Dinani pa Networking tabu.
Sankhani netiweki yanu kuchokera ku menyu yotsitsa kapena sankhani "Lowani netiweki ina ..." ndikulowetsa SSID yake. Sankhani njira yotsimikizira bizinesi yomwe netiweki yanu imagwiritsa ntchito. Njira zothandizira ndi:
EAP-TTLS yokhala ndi TLS EAP-TTLS yokhala ndi MS-CHAP v2 EAP-TTLS yokhala ndi MD5 EAP-PEAP yokhala ndi MS-CHAP v2 EAP-TLS
Iliyonse mwa njirazi imafunikira dzina lolowera ndi mawu achinsinsi, ndipo kutengera masinthidwe anu enieni angafunikire satifiketi files kapena zosankha zina. Onani zolemba za IT za malo anu kapena funsani woyang'anira IT wanu kuti mumve zambiri za kukhazikitsidwa kwa netiweki yanu.
Ethernet: Lumikizani loboti yanu ku netiweki switch kapena hub ndi chingwe cha Ethernet. Mukhozanso kulumikiza mwachindunji ku doko la Efaneti pa kompyuta yanu, kuyambira mu mtundu wa robot 7.1.0.
USB: Lumikizani chingwe cha USB A-to-B choperekedwa ku doko la USB-B la loboti komanso potsegula pa kompyuta yanu. Gwiritsani ntchito chingwe cha USB B-to-C kapena adapter ya USB A-to-C ngati kompyuta yanu ilibe doko la USB-A.
Kuti mupitilize kuyika, kompyuta yolumikizidwa iyenera kukhala ndi Opentrons App yoyika ndikuyendetsa. Kuti mudziwe zambiri pakuyika Opentrons App, onani gawo la App Installation la mutu wa Software and Operation.
Ikani zosintha zamapulogalamu
Tsopano popeza mwalumikiza netiweki kapena kompyuta, lobotiyo imatha kuyang'ana zosintha zamapulogalamu ndi firmware ndikutsitsa ngati pakufunika. Ngati pali zosintha, zingatenge mphindi zingapo kuti muyike. Zosintha zikatha, loboti iyambiranso.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
Gwiritsirani ntchito Emergency Stop Pendant
Lumikizani Emergency Stop Pendant (E-stop) ku doko lothandizira (AUX-1 kapena AUX-2) kumbuyo kwa loboti.

Isanayambe kapena itatha kulumikiza Emergency Stop Pendant.
Kulumikiza ndi kuyatsa E-stop ndikofunikira kuti muphatikize zida ndikuyendetsa ma protocol pa Flex. Kuti mudziwe zambiri pakugwiritsa ntchito E-stop panthawi ya robot, onani gawo la Emergency Stop Pendant la mutu wa System Description.
Perekani dzina loboti yanu
Kutchula loboti yanu kumakupatsani mwayi wozindikira mosavuta pamalo omwe muli labu. Ngati muli ndi maloboti angapo a Opentrons pa netiweki yanu, onetsetsani kuti mwawapatsa mayina apadera. Mukatsimikizira dzina la loboti yanu, mudzatengedwera ku Opentrons Flex Dashboard yanu. Mwachiwonekere sitepe yotsatira yomwe mungafune kuchita ndikuyika zida, zomwe zili ndi gawo lotsatira.
2.4 Kuyika ndi kusanja zida
Mukakhazikitsa koyamba loboti, chotsatira ndikuyika zida ku loboti ndikuziyesa.
Kuti muyike chida, dinani kaye pa Instruments pa touchscreen kapena pitani ku gawo la Pipettes ndi Modules pazithunzi zatsatanetsatane za chipangizocho mu Opentrons App. Sankhani phiri lopanda kanthu ndikusankha Ikani Pipette kapena Ikani Gripper. Ngati phiri lomwe mukufuna kugwiritsa ntchito lakhala kale, muyenera kuchotsa pipette kapena gripper poyamba.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
Zindikirani: Kuyika kwathunthu ndi chimodzimodzi posatengera kuti mumagwiritsa ntchito chophimba kapena Opentrons App. Chida chilichonse chomwe mungayambire chimawongolera kuyikapo mpaka mutamaliza kapena kuletsa.
Mukayamba pa touchscreen, pulogalamuyi idzawonetsa lobotiyo ngati "yotanganidwa". Mukayamba mu pulogalamuyi, chojambula chojambula chidzawonetsa modal yosonyeza kuti kuyika zida kukuchitika.
Kuyika kwake kwenikweni kumasiyanasiyana malinga ndi chida chomwe mukuchilumikiza, monga momwe zafotokozedwera m'zigawo zomwe zili pansipa. Zida zonse zili ndi njira yosinthira yokha, yomwe muyenera kuchita mukangokhazikitsa.
Kuyika kwa Pipette
Mukayika pipette, mudzawongoleredwa ndi njira zotsatirazi pa touchscreen kapena Opentrons App.
1. SANKHANI PIPETTE TYPE Sankhani pakati pa 1- kapena 8-Channel Pipette ndi 96-Channel Pipette. Kulumikiza 96-Channel Pipette kumafuna masitepe owonjezera pang'ono chifukwa imamangiriza mbale yapadera yoyikira yomwe imadutsa mapiri onse awiri.
2. KONZEKERANI KUYANG'ANIRA Chotsani labware pamalopo ndikuyeretsa malo ogwirira ntchito kuti kumangiriza ndikuwongolera mosavuta. Sonkhanitsaninso zida zofunika, monga choyezera, hex screwdriver, ndi mounting plate (za 96-Channel Pipette).
3. LUMIKIZANI NDI KUTETEZA PIPETTE Gantry idzasunthira kutsogolo kwa robot kuti muthe kulumikiza pipette.
1- ndi 8-Channel Pipettes amalumikizana mwachindunji ndi phiri la pipette. Pipette ya 96-Channel imafuna mbale yokwera. Kuti mumangirize mbale yoyikira, muyenera choyamba kulumikiza chonyamulira cha z-axis chokwera kumanja kwa pipette.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
Lumikizani pipette ku phiri losankhidwa la pipette ndikuteteza zomangira zake.
4. KUTHENGA ZOCHITIKA ZONSE Kuti muyese pipette, sungani kafukufuku woyezera pamphuno yoyenera ya pipette. Pipette idzasuntha yokha kuti ikhudze mfundo zina pa sitimayo ndikusunga izi kuti zigwiritsidwe ntchito mtsogolo. Kuwongolera kukatha ndipo mwachotsa kafukufuku, pipette idzakhala yokonzeka kugwiritsidwa ntchito muzotsatira.
Kuyika kwa Gripper
Mukayika cholumikizira, mudzawongoleredwa panjira zotsatirazi pazithunzi kapena pa Opentrons App.
1. KONZEKERANI KUIKHALITSA Chotsani labware pamalopo ndikuyeretsa malo ogwirira ntchito kuti kulumikiza ndi kuwongolera kukhale kosavuta. Komanso sonkhanitsani hex screwdriver yofunikira ndikuwonetsetsa kuti pini yowongolera ili m'malo ake osungira pa chogwirira.
2. LUMIKIZANI NDI KUTETEZA GRIPPER Gantry idzasunthira kutsogolo kwa robot kuti mugwirizane ndi chogwirira. Lumikizani chogwirizira ku chokwera chowonjezera ndikuteteza zomangira zake.
3. THAWANI ZOCHITA ZONSE Kuti muyese chogwirira, ikani pini yoyeserera kutsogolo kwa nsagwada. Chogwirizira chimangosuntha kuti chikhudze mfundo zina pa desiki ndikusunga ma calibration awa kuti adzagwiritse ntchito mtsogolo. Kenako bwerezaninso zomwezo ndi pini ya calibration kumbuyo kwa nsagwada. Kuwongolera kukatha ndipo mwabwezeretsanso piniyo pamalo ake osungira, chogwirizira chikhala chokonzeka kugwiritsidwa ntchito pama protocol.
2.5 Kusamuka
Gawoli limapereka malangizo ndi malangizo amomwe mungasunthire loboti yanu ya Opentrons Flex pa mtunda waufupi komanso wautali.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
Zoyenda zazifupi
Kusuntha pang'ono kumatenga mtunda wautali kuchokera "tiyeni tingosuntha pang'ono" kupita kudutsa labu, kutsika muholo, kapena pansi pa nyumba yanu. Muzochitika izi, mutha kusuntha Flex yanu pamanja. Kunyamula pa ngolo yamanja ndi njira yabwino.
Chenjezo: The Flex imalemera 88.5 kg. Chifukwa cha zimenezi, pamafunika anthu awiri kuti anyamule ndi kulisuntha bwinobwino.
Gwiritsirani ntchito zonyamula katundu kuti musunthire Flex yanu kupita kumalo atsopano, pafupi. Kukweza ndi kunyamula Flex ndi manja ake ndi njira yoyenera yosunthira loboti mtunda waufupi. Chotsani zogwirira ntchito ndikuzisunga mu User Kit mukatha kusuntha. Pofuna kupewa kuwononga loboti, nthawi zonse gwiritsani ntchito zogwirizira zonyamulira kuti munyamule ndikusuntha. Osagwira chimango kuti mukweze kapena kusuntha loboti yanu.
Kuyenda mtunda wautali
Kusuntha mtunda wautali kumachotsa Flex yanu kuchokera ku yunivesite, malo, kapena malo anu. Kudutsa tawuni, ku mzinda watsopano, chigawo, chigawo, kapena dziko zonse ndi zakaleampkusuntha kwa mtunda wautali. Pankhaniyi, muyenera kunyamula Flex kuti muyiteteze ku zinthu, kugwedezeka, ndi mayendedwe ovuta omwe angachitike mukuyenda.
Ngati mwasunga bokosi lotumizira ndi zothandizira zamkati zomwe zidabwera ndi Flex yanu, mutha kuyiyikanso muzinthu izi kuti muyende mtunda wautali. Tsatirani masitepe a unboxing mobwerera m'mbuyo kuti mukonzekere Flex yanu kuti isamuke mtunda wautali. Kwenikweni, muyenera:
Chotsani mphamvu ndi chingwe cha netiweki, ngati cholumikizidwa. Chotsani zida zonse zolumikizidwa ndi labware. Bweretsaninso mbale zapansi. Tsekani chotchinga (onani General Moving Advice gawo pansipa). Chotsani ndi kusunga mazenera mapanelo.
Ngati mudasunga kreti loyambirira:
Lumikizaninso chimango chotumizira ku Flex ndikuchitchinjiriza pamapallet pogwiritsa ntchito mabaketi a L. Onjezerani padding ndikugwirizanitsanso bokosi lotumizira.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
Ngati mulibe crate yoyambirira ndi zinthu zina zogwirizana nazo, funsani kampani yodziwika bwino yotumiza katundu. Atha kukukonzerani kulongedza, mayendedwe, ndi njira yobweretsera.
Malangizo onse osuntha
LULUKANITSA ZIKABWI ZA MPHAMVU NDI NETWORK Musanasamutse Flex yanu, musaiwale: Zimitsani magetsi ndikumachotsa pamagetsi. Lumikizani Efaneti kapena USB chingwe, ngati ntchito.
TSEKANI GANTRY Musanasunthe Flex yanu, lowetsaninso zomangira zotsekera kuti mutseke chotchinga. Malo otsekera gantry ali: Kumanzere njanji pafupi ndi kutsogolo kwa loboti. Pansi pa mkono woyima wa gantry. Kumanja kwa njanji pafupi ndi kutsogolo kwa robot. Kutseka gawo ili la gantry kumafuna yaying'ono
bulaketi lalanje ndi zomangira ziwiri zokhoma.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
HOME GANTRY Mwina simukufuna kutseka chitseko ngati mukusuntha loboti kupita kumalo oyandikana nawo. Ngati mwaganiza kuti musatseke, gwiritsani ntchito chotchinga chokhudza kapena Opentrons App kuti mutumize gantry pamalo ake akunyumba musanayitsitse. Kuti mukhazikitse gantry kudzera pa touchscreen, dinani menyu ya madontho atatu () ndiyeno dinani Home gantry. Kuti mukhazikitse gantry kudzera pa Opentrons App: Dinani Zida. Dinani pa Flex yanu pamndandanda wazida. Dinani menyu ya madontho atatu () ndikudina Home gantry.
CHOTSANI MAMODULE Ma module amkati ndi zomata zina zimawonjezera kulemera kwa Flex yanu. Zimakhudzanso malo apakati pa mphamvu yokoka ya loboti, zomwe zimapangitsa kuti limve ngati "tippy" polikweza. Kuti muchepetse komanso kusanja loboti, chotsani zida zilizonse zomwe zalumikizidwa ndi labware musanayitenge.
Bwezeretsaninso mipata ya DECK Tikupangira kuti mulumikizanenso ndi mipata ya sitimayo kuti musunthe mtunda wautali. Kuteteza mipata m'malo awo oyamba kumathandiza kupewa kutaya mwangozi. Kuyikanso malo otsetsereka kuti muziyenda pang'ono kuzungulira labu ndikosankha.
KUSINTHA KWAMBIRI KWAMBIRI Muyenera kukonzanso zida zilizonse ndi ma module mutatha kuziyikanso. Kuti mumve zambiri zakusintha kwa ma module, onani mutu wa Ma module.

Mtengo wa OPENTRONS FLEX

MUTU 2: KUYANG'ANIRA NDI KUSINTHA
Malingaliro omaliza okhudza kusuntha
Flex yanu ndi makina olimba komanso opangidwa bwino, komanso ndi chida cholondola chasayansi chomwe chimapangidwa kuti chizitha kupirira. Chotsatira chake, muyenera kuchisamalira mosamala mukachisamutsa m'dera lanu lantchito kapena kutumiza dziko lonselo. Izi zikutanthauza kutsatira malangizo omwe aperekedwa pano ndikugwiritsa ntchito nzeru zanu za momwe munganyamulire chipangizo chokwera mtengo cha labotale. Mfundo yofunika kwambiri: mukasuntha Flex yanu, lakwitsani mbali yosamala ndi zowonjezera zowonjezera.
Ngati muli ndi mafunso kapena nkhawa zakusamutsa Flex yanu, titumizireni ku support@opentrons.com.

Mtengo wa OPENTRONS FLEX

MUTU 3
Kufotokozera Kwadongosolo

Mutuwu ukufotokoza machitidwe a Hardware a Opentrons Flex, omwe amayang'anira mawonekedwe ake a labotale. Sitimayo, gantry, ndi zida za Opentrons Flex zimathandiza kugwiritsa ntchito zida zamadzimadzi zolondola komanso zogwiritsira ntchito labware. Chojambula cha pachipangizochi chimathandizira kuyendetsa ma protocol ndikuyang'ana momwe robot ilili popanda kubweretsa kompyuta yanu ku benchi ya labu. Kulumikizika kwa mawaya ndi opanda zingwe kumathandizira kuwongolera kowonjezera kuchokera ku Opentrons App (onani mutu wa Software and Operation kuti mumve zambiri) ndikukulitsa mawonekedwe amtunduwu pomangirira zotumphukira (onani mutu wa Ma module).

3.1 Zigawo zakuthupi

Kamera

Kuwala kwa Status

Zenera logwira

Chimango

Gantry Deck
Khomo Lakutsogolo
Malo azinthu zakuthupi za Opentrons Flex.

Mbali za Windows Handle Caps

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU

Chimango ndi mpanda
Chojambula cha loboti ya Opentrons Flex imapereka kukhazikika komanso kuthandizira pamapangidwe ake komanso gantry. Ma subsystems onse amakina amakhalapo ndikuyikidwa pa chimango chachikulu. Chimangocho chimapangidwa makamaka ndi chitsulo chachitsulo ndi aluminiyamu extrusions.
Chitsulo chachitsulo chimakhala ndi zitseko zamawindo am'mbali ndi khomo lakutsogolo lopangidwa ndi polycarbonate yowonekera yomwe imakulolani kuwona zomwe zikuchitika mkati mwa Flex. Zitseko za khomo lakumaso zimatseguka kuti zitheke kulowa mkati mwa dongosolo. Ndi chitseko chakutsogolo chotseguka, mutha kulumikiza zida, ma module, ndi zida zamasitepe; konzani sitimayo pamaso pa protocol; kapena kusintha mkhalidwe wa sitimayo panthawi ya protocol.
Mizere yoyera ya LED m'mphepete mwa pamwamba pa chimango imapereka kuwala kowoneka bwino kwa mapulogalamu. Kamera ya 2-megapixel imatha kujambula sitimayo ndi malo ogwirira ntchito pojambulira ndikutsata ma protocol.

Deck ndi malo ogwira ntchito
Sitimayo ndi aluminiyamu yopangidwa ndi makina pomwe ma protocol asayansi amapangidwira. Sitimayo ili ndi mipata yayikulu 12 ya ANSI/SLAS-format yomwe ingasinthidwenso kuti igwire labware, ma module, ndi zogwiritsidwa ntchito. Malo otsetsereka amazindikiridwa ndi makina olumikizirana, okhala ndi slot A1 kumanzere kumanzere ndi slot D3 kutsogolo kumanja.

Slot Expansion (kwa Thermocycler) Malo Ogwirira Ntchito

StagArea

Madera a sitimayo mkati mwa Flex.

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU
Malo ogwirira ntchito ndi malo owoneka pamwamba pa sitimayo yomwe imapezeka kwa pipetting. Labware yoyikidwa mu mipata A1 kudutsa D3 ali pamalo ogwirira ntchito.
Opentrons Flex imabwera ndi mipata yochotsamo pamaudindo onse 12 omwe amagwira ntchito. Chigawo chilichonse chimakhala ndi zikwatu zamakona zoyika labware pamalopo.
Mutha kusinthanso sitimayo posintha mipata ndi zida zina, kuphatikiza zinyalala zosunthika, zinyalala za zinyalala, ndi ma module caddies. Malo okulitsa kumbuyo kwa A1 amangogwiritsidwa ntchito kupanga malo owonjezera a Thermocycler Module, yomwe imakhala ndi mipata A1 ndi B1.
Zindikirani: Malo otsetsereka amatha kusinthana mkati mwa ndime (1, 2, kapena 3) koma osati kudutsa mizati; ndime 1 ndi gawo 3 mipata ndi zidutswa zosiyana ngakhale kukula kwake kofanana. Mutha kudziwa ndime yomwe kagawo imalowera poyang'ana kanema wabuluu kumanzere kumanzere.
Muyenera kusiya malo osungiramo malo omwe mukufuna kuyika labware yoyimirira. Sitimayo ndi zinthu zomwe zimayikidwapo zimakhalabe zokhazikika, pokhapokha zitasunthidwa ndi chogwirizira kapena kulowererapo pamanja.
StagMalo
Ataging area ndi malo owonjezera kumanja kwa sitimayo. Mutha kusunga labware pamalowa mutakhazikitsa stagmalo mipata. Labware yoyikidwa mu mipata A4 kudzera D4 ali mu staging area. Flex pipettes sangathe kufika mu staging, koma chogwirizira chikhoza kunyamula ndi kusuntha labware kupita ndi kuchokera kumalo ano. Kuwonjezera malo owonjezera kumathandizira kuti malo ogwirira ntchito azikhala opezeka pazida zomwe zimagwiritsidwa ntchito muzochita zanu zokha.
Stagmalo otsetsereka amaphatikizidwa ndi masinthidwe ena ogwirira ntchito ndipo amapezekanso kuti agulidwe kuchokera ku https://shop.opentrons.com.

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU

Staging Area ndi mipata Anaika

Zosintha zapa deck
Zosintha ndi zinthu za Hardware zomwe zimalowetsa mipata yokhazikika. Amakulolani kuti musinthe makonda anu ndikuwonjezera magwiridwe antchito ku Flex yanu. Pakadali pano, zida zamasitepe zikuphatikiza stagmipata, bin ya zinyalala mkati, ndi chute kunja zinyalala. Mutha kukhazikitsa zosintha m'malo ochepa chabe. Pansipa pali mndandanda wa malo omwe ali pagulu lililonse.

Kukonzekera Staging area mipata Zinyalala bin Zinyalala chute Zinyalala chute ndi staging area slot

Mipata A3D3 A1D1 ndi A3-D3 D3 D3 yekha

Zosintha zilibe mphamvu. Zilibe zida zamagetsi kapena zamakina zomwe zimalumikizana ndi malo omwe ali pano komanso malo osungira maloboti. Izi zikutanthauza kuti muyenera kugwiritsa ntchito mawonekedwe amasinthidwe kuti adziwitse Flex zomwe zimayikidwa pa sitimayo komanso komwe zili.

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU

Mutha kulumikizana ndi zosintha zamasinthidwe kuchokera pa touchscreen pogwiritsa ntchito madontho atatu () menyu komanso kuchokera ku Opentrons App. Onani gawo la Deck Configuration la chaputala cha Software and Operation kuti mumve zambiri zamomwe mungakhazikitsire sitimayo kuchokera pa touchscreen.

Zinyalala chute
Opentrons Flex Waste Chute imasamutsa zakumwa, maupangiri, zopangira nsonga, ndi mbale zachitsime kuchokera pamalo otsekera a Flex kupita kumalo osungira zinyalala omwe amayikidwa pansi pa kutseguka kwake kwakunja. Chute cha zinyalala chimamangirira ku adapter ya mbale yomwe imakwanira pa slot D3. Imabweranso ndi zenera lapadera la theka lomwe limalola chute kutuluka kutsogolo kwa robot.
Zigawo za chute zinyalala.

Kuphimba Deck Plate Adapter

Waste Chute
Deck Plate Adapter yokhala ndi StagArea

Stagmalo mipata
Staging area slots ndi zidutswa za ANSI/SLAS zomwe zimalowetsa mipata yokhazikika mugawo 3 ndikuwonjezera mipata yatsopano ku s.taging area - zonse popanda kutaya malo mu malo ogwira ntchito. Mutha kukhazikitsa kagawo kamodzi kapena mipata inayi kuti mupange gawo latsopano (A4 mpaka D4) kumanja kwa sitimayo. Dziwani, komabe, kuti m'malo mwa deck slot A3 kumafuna kusuntha nkhokwe ya zinyalala. Powonjezera stagPokhala ndi malo otsetsereka, loboti yanu ya Flex imatha kusunga labware yambiri ndikugwira ntchito bwino.

Mtengo wa OPENTRONS FLEX

Flex staging area slot.
49

MUTU 3: KUFOTOKOZERA ZINTHU
Slot INSTALLATION
Kuti muyike, chotsani zomangira zomwe zimangiriza kagawo wamba pa sitimayo ndikusintha ndi staging area slot. Pambuyo kukhazikitsa, gwiritsani ntchito touchscreen kapena Opentrons App kuti muuze loboti yomwe mwawonjezera ngatitagmalo olowera ku deck.
Kuyika ngatitaging area slot.

Slot COMPATIBILITY Stagmalo otsetsereka amagwirizana ndi zida za Flex, ma module, ndi labware zomwe zalembedwa pansipa.

Flex chigawo cha Gripper Pipettes Modules
Labware

Stagkuyanjana kwa dera
Flex Gripper imatha kusuntha labware kupita kapena kuchokera ku stagmalo mipata.
Flex pipettes sangathe kufika ku staging area. Gwiritsani ntchito chophatikizira kuti musunthe nsonga zopangira ndi labware kuchokera ku staging malo ku malo ogwira ntchito pamaso pa pipetting.
Magnetic Block GEN1 ikhoza kuyikidwa mugawo 3 pamwamba pa astaging area slot. Ma module sagwiritsidwa ntchito mugawo 4.
Ma module amagetsi monga Heater-Shaker ndi Temperature Module amalowa mu makadi omwe atha kuikidwa mugawo 3. Simungawonjezere ngatitaging area slot ku malo okhala ndi module caddy.
StagMalo otsetsereka amakhala ndi miyeso yofanana ya ANSI/SLAS ngati mipata yokhazikika. Gwiritsani ntchito labware yogwirizana ndi gripper mu stagmalo, kapena onjezani pamanja ndikuchotsa zolemba pamalopo.

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU
Kachitidwe kamayendedwe
Chophatikizidwa ndi chimangocho ndi gantry, yomwe ndi kayendedwe ka robot ndi kachitidwe kake. Gantry imasuntha padera pa x- ndi y-axis kuti ikhazikitse pipettes ndi gripper pamalo enieni opangira protocol. Kuyenda motsatira nkhwangwa izi ndikolondola mpaka kufupi ndi 0.1 mm. Gantry imayendetsedwa ndi 36 VDC hybrid bipolar stepper motors. Momwemonso, zophatikizidwa ndi gantry ndi mapiri a pipette ndi phiri lowonjezera. Izi zimayenda motsatira z-axis kuti ikhazikitse ma pipette ndi gripper pamalo olondola kuti protocol ikwaniritsidwe. Kuyenda motsatira mbali iyi kumayendetsedwa ndi 36 VDC hybrid bipolar stepper motors. Zamagetsi zomwe zili mu gantry zimapereka mphamvu za 36 VDC ndi mauthenga kwa pipettes ndi gripper, zikaphatikizidwa.
Gantry

Pipette Mounts

Mount Extension

Malo a chida amakwera pa Flex.

Mawonekedwe a Touchscreen ndi ma LED
Mawonekedwe oyambira ogwiritsa ntchito ndi 7-inch LCD touchscreen, yomwe ili kumanja kwa loboti. Chophimbacho chimakutidwa ndi Gorilla Glass 3 kuti chikande komanso kukana kuwonongeka. Pezani zambiri za Flex pomwepo pa touchscreen, kuphatikiza:

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU

Kuwongolera kwa Protocol Kukhazikitsa, kukhazikitsa, ndi kuyang'anira kasamalidwe ka Labware Zokonda pa robot Mapulogalamu a System ndi zosintha za firmware Zolemba zantchito ndi zidziwitso zolakwika
Kuti mumve zambiri pakugwiritsa ntchito Flex kudzera pa touchscreen, onani gawo la Touchscreen Operation la mutu wa Software and Operation.
Kuwala kwake ndi kadulidwe ka ma LED pamwamba pa loboti yomwe imapereka chidziwitso pang'onopang'ono za loboti. Mitundu yosiyanasiyana ndi zowunikira zimatha kuyankhulana bwino, kulephera, kapena kusagwira ntchito mosiyanasiyana:

Mtundu wa LED White Neutral states
Green Normal states
Blue Mandatory imanena kuti Yellow Abnormal states Red Emergency states

Chithunzi cha LED Solid Pulsing
Kuphethira kawiri
Kuthamanga Kolimba Kwambiri

Maloboti
Yayatsidwa koma osagwiritsa ntchito protocol Roboti imakhala yotanganidwa (mwachitsanzo, kukonzanso mapulogalamu kapena firmware, kukhazikitsa protocol run, kuletsa protocol run) Kuchita kwatha (mwachitsanzo, kusungidwa kwa protocol, kusinthidwa kwa mapulogalamu, chida cholumikizidwa kapena kuchotsedwa) Protocol yatha. Protocol yayimitsidwa

Zolimba

Vuto la pulogalamu

Kuthwanima katatu, mobwerezabwereza

Zolakwika pathupi (mwachitsanzo, kuwonongeka kwa zida)

Kuwala kwa mawonekedwe kumathanso kuyimitsidwa pamakonzedwe a robot.

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU
3.2 Pipettes
Opentrons pipettes ndi zida zosinthika zomwe zimagwiritsidwa ntchito kusuntha zakumwa m'dera lonse logwira ntchito panthawi ya ma protocol. Pali ma pipette angapo a Opentrons Flex, omwe amatha kunyamula ma voliyumu kuchokera ku 1 µL mpaka 1000 µL mumayendedwe 1, 8, kapena 96:
Opentrons Flex 1-Channel Pipette (1 µL) Opentrons Flex 50-Channel Pipette (1 µL) Opentrons Flex 5-Channel Pipette (1000 µL) Opentrons Flex 8-Channel Pipette (1 µL) Opentrons 50 Flex 8x µL) Opentrons 5 Flex 1000x96 L)
Ma pipettes amangirira ku gantry pogwiritsa ntchito zomangira zomangira kutsogolo kwa pipette. 1-channel ndi 8-channel pipettes aliyense amakhala limodzi pipette phiri (kumanzere kapena kumanja); pipette ya 96-channel imakhala ndi mapiri onse awiri. Kuti mumve zambiri pakuyika ma pipette, onani Kuyika kwa Instrument and Calibration.

Kumangidwa Kwabata
Zomangira

Zomangira Zomangamanga

Kutaya

Nozzles (Zowonjezera za O-mphete)

Nozzles (Zokhazikika za O-mphete)

Malo a zigawo za 1-, 8-, ndi 96-channel pipettes.

Kutaya

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU

Ma pipette amatenga nsonga za pulasitiki zotayidwa pozikanikiza pamitsuko ya pipette, ndiyeno gwiritsani ntchito nsongazo kulakalaka ndi kugawira zakumwa. Kuchuluka kwa mphamvu zonse zomwe zimafunika kuti munthu azinyamula zikuchulukirachulukira pamene malangizo ambiri amatengedwa nthawi imodzi. Kwa maupangiri ang'onoang'ono, pipette imamangiriza nsonga pokankhira nsonga ya pipette kunsonga. Kuti mukwaniritse mphamvu zofunikira kuti mutenge nsonga zonse, pipette ya 96-channel imakokeranso nsongazo m'mwamba pamphuno. Kukoka kumeneku kumafuna kuyika zoyika nsonga mu adaputala ya nsonga, m'malo molunjika panjanji. Kutaya maupangiri (kapena kuwabwezera ku rack yawo), makina ojambulira pipette amakankhira nsonga kuchokera pamphuno.

Pipette specifications
Opentrons Flex pipettes adapangidwa kuti azigwira ma voliyumu osiyanasiyana. Chifukwa cha kuchuluka kwawo konse, amatha kugwiritsa ntchito maupangiri angapo, omwe amakhudza momwe amagwirira ntchito zamadzimadzi. Opentrons yayesa mapaipi a Flex kuti akhale olondola komanso olondola pamaphatikizidwe angapo ansonga ndi kuchuluka kwamadzimadzi:

Pipette
Flex 1-Channel
50 μl
Flex 1-Channel
1000 μl
Flex 8-Channel
50 μl
Flex 8-Channel
1000 μl

Tip Mphamvu 50 µL 50 µL 50 µL 50 µL 50 µL 200 µL 1000 µL 50 µL 50 µL 50 µL 50 µL 50 µL 200

Kuyesedwa Volume 1 µL 10 µL 50 µL 5 µL 50 µL
200µL 1000µL
1 µL 10 µL 50 µL 5 µL 50 µL 200 µL 1000 µL

Zolondola %D 8.00% 1.50% 1.25% 5.00% 0.50% 0.50% 0.50% 10.00% 2.50% 1.25% 8.00% 2.50% 1.00% 0.70%.

Zolondola %CV 7.00% 0.50% 0.40% 2.50% 0.30% 0.15% 0.15% 8.00% 1.00% 0.60% 4.00% 0.60% 0.25% 0.15%

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU

Flex 96-Channel
1000 μl

50 µL 50 µL 200 µL 1000 µL

5 µL 50 µL 200 µL 1000 µL

10.00% 2.50% 1.50% 1.50%

5.00% 1.25% 1.25% 1.50%

Kumbukirani izi zolondola posankha malangizo a pipette. Nthawi zambiri, kuti mupeze zotsatira zabwino muyenera kugwiritsa ntchito malangizo ang'onoang'ono omwe amakwaniritsa zosowa za protocol yanu.

Zindikirani: Opentrons amayesa ma volumetric a ma pipette a Flex kuti atsimikizire kuti akukwaniritsa zolondola komanso zolondola zomwe zalembedwa pamwambapa. Simuyenera kuwerengera kuchuluka kwa ma pipette anu musanagwiritse ntchito. Mukungoyenera kupanga ma calibration. Onani gawo lotsatira, komanso gawo la Pipette Installation la mutu wa Kukhazikitsa ndi Kusuntha, kuti mudziwe zambiri.
Ntchito za Opentrons Care ndi Opentrons Care Plus zikuphatikiza kusintha kwa pipette chaka chilichonse ndi ziphaso zowongolera. Onani gawo la Servicing Flex la mutu wa Maintenance and Service kuti mumve zambiri.

Pipette calibration
The User Kit imaphatikizapo kufufuza kwachitsulo kwa pipette, komwe mumagwiritsa ntchito panthawi yoyendetsa. Mukamayendetsa ma protocol, sungani kafukufukuyu pachogwira maginito pa nsanamira yakutsogolo ya loboti. Pa nthawi ya calibration, phatikizani kafukufukuyo pamphuno yoyenera ndikutseka m'malo mwake. Loboti imasuntha kafukufukuyo kumalo oyeserera pa sitimayo kuti ayeze pomwe pipetteyo ili.
Adapter ya nsonga ya Pipette
Sitima zapaipi za Opentrons Flex 96 zokhala ndi ma adapter nsonga anayi. Awa ndi mabatani a aluminiyamu opangidwa molondola omwe mumayika pa sitimayo. Ma adapter amakhala ndi ma racks a Flex 50 L, 200 L, ndi 1000 μL.

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU
Chifukwa cha mphamvu yomwe ikukhudzidwa, pipette ya 96-channel imafuna adaputala kuti imangirire nsonga zonse bwino. Panthawi yophatikizira, pipette imayenda pamwamba pa adaputala, imatsikira pazikhomo, ndikukokera nsonga pazipiipi pokweza adaputala ndi nsonga choyikapo. Kukoka nsonga, m'malo mokankhira, kumapereka mwayi wofunikira kuti muteteze nsonga za pipettes ndikuletsa kugwedeza pamwamba pa sitimayo. Ikamalizidwa, pipette ya 96-channel imatsitsa adaputala ndikuyika nsonga yopanda kanthu pamtunda. Onani gawo la Malangizo ndi nsonga pamutu wa Labware kuti mudziwe zambiri.
Kujambula pang'ono nsonga
Pipette ya 96-channel imatha kutenga nsonga zonse kapena malangizo ochepa. Izi zimawonjezera kuchuluka kwa mapulogalamu omwe mungathe kuchita ndi pipette ya 96-channel, chifukwa imakhala ndi mapepala onse awiri.
Pakadali pano, pipette ya 96-channel imathandizira kujambula pang'ono kwa malangizo 8 pamasanjidwe amzati. Pakusintha uku, pipette imagwiritsa ntchito milomo yake yakumanzere kuti itenge nsonga kuchokera kumanja kupita kumanzere kuchokera ku nsonga, kapena ma nozzles ake kumanja kuti atenge nsonga kumanzere kupita kumanja kuchokera pachimake.
Mukatenga nsonga zosakwana 96 kuchokera pachibowo, choyikapo chiyenera kuyikidwa mwachindunji pa sitimayo, osati mu adaputala.
Pipette sensors
Opentrons Flex pipettes ali ndi masensa angapo omwe amazindikira ndi kulemba deta za momwe pipette ilili ndi malangizo aliwonse omwe adatenga.
ZOGWIRITSA NTCHITO
Kuphatikizana ndi kafukufuku wachitsulo kapena nsonga ya conductive, masensa a capacitance amazindikira pamene pipette ikugwirizana ndi chinachake. Kuzindikira kukhudzana pakati pa kafukufuku wachitsulo ndi sitimayo kumagwiritsidwa ntchito poyesa kuwongolera kwa pipette ndi ma module calibration.
Ma pipette a 1-channel ali ndi sensa imodzi ya capacitance, pamene mapaipi amtundu wambiri ali ndi awiri: pazitsulo 1 ndi 8 za 8-channel pipettes, ndi pa channel 1 ndi 96 (maudindo A1 ndi H12) a pipette ya 96.

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU

OPTICAL TIP PRESENCE SENSOR
Kusintha kwa photointerruptor kumazindikira malo a pipette's ejector mechanism, kutsimikizira ngati nsonga zinatoledwa bwino kapena kugwetsedwa. 1-channel, 8-channel, ndi 96-channel pipettes onse ali ndi sensa imodzi ya kuwala yomwe imayang'anira nsonga zamtundu uliwonse.
Pipette firmware zosintha
Opentrons Flex imangosintha firmware ya pipette kuti ikhale yogwirizana ndi mtundu wa pulogalamu ya robot. Zosintha za firmware za Pipette zimakhala zachangu, ndipo zimachitika nthawi iliyonse:
Mumangirira pipette. Roboti imayambiranso.
Ngati, pazifukwa zilizonse, mapulogalamu anu a pipette ndi mapulogalamu a robot achoka pa kulunzanitsa, mukhoza kusintha pamanja firmware mu Opentrons App.
1. Dinani Zida. 2. Dinani pa Flex yanu pamndandanda wa zida. 3. Pansi pa Zida ndi Ma modules, pipette ya kunja-kulunzanitsa idzawonetsa kuwerenga kwa banner yochenjeza
"Zosintha za firmware zilipo." Dinani Update tsopano kuti muyambe kusintha.
Mutha view mtundu wa firmware womwe wakhazikitsidwa pano wa pipette iliyonse yolumikizidwa. Pa touchscreen, kupita Zida ndikupeza dzina pipette. Mu Opentrons App, pezani khadi la pipette pansi pa Zida ndi Ma modules, dinani menyu ya madontho atatu (), ndiyeno dinani About pipette.

3.3 Chipolopolo
The gripper amasuntha labware m'dera lonse ntchito ndi stagmalo pakuchita ma protocol. Chogwirizira chimamangiriza ku phiri lokulitsa, lomwe ndi losiyana ndi mapiri a pipette; gripper angagwiritsidwe ntchito ndi kasinthidwe pipette. Kuti mumve zambiri pakuyika gripper, onani Kuyika kwa Instrument and Calibration.
Chogwirizira chimatha kusuntha labware kudutsa sitimayo ndikuyika kapena kuyimitsa ma module. Chogwirizira chimatha kuwongolera mbale zina zachitsime, mbale zakuya, ndi zotsekera. Kuti mumve zambiri za zomwe gripper imatha kusuntha, onani gawo la Labware ndi Opentrons Flex Gripper la mutu wa Labware, kapena onani Laibulale ya Opentrons Labware.

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU

Zolemba za Gripper
Nsagwada zimagwira ntchito yoyambira ya chogwirira, chomwe ndikutsegula kapena kutseka zopalasa ziwiri zofananira kuti zigwiritse kapena kutulutsa mphamvu m'mbali mwa labware. Kuyenda kwa nsagwada kumayendetsedwa ndi 36 VDC brushed motor yolumikizidwa ndi rack-and-pinion gear system.
Kuti asunthe chidutswa cha labware chomwe chagwidwa ndi nsagwada, gantry imakweza chogwirizira pa z-axis, ndikuchisuntha chammbali, ndikuchitsitsa kumalo atsopano a labware.
Malo a zigawo za gripper.

Zomangira zomangira
Calibration pini ya Jaws Paddles

Gripper calibration
Chogwirizira chimakhala ndi pini yowongolera zitsulo. Pini ya calibration ili m'malo osungiramo malo omwe ali pansi pa gripper. Maginito amagwirizira pini m'malo mwake. Kuti muchotse pini yoyeserera, igwireni ndi zala zanu ndikukoka modekha. Kuti mulowetse piniyo, ibwezereni mu kagawo kosungirako. Mudzadziwa kuti ndi yotetezeka ikalowa m'malo mwake.
Mukamayesa chogwirira, sungani chikhomo ku nsagwada iliyonse motsatana. Loboti imasuntha piniyo kuti ifike pamalo oyeserera kuti ayeze pomwe pali chogwiriracho.
Panthawi yoyendetsa protocol, ikani pini m'malo ake osungira kuti mutetezeke. Lumikizanani nafe support@opentrons.com ngati mutaya pini yoyeserera.

Zosintha za Gripper firmware
Opentrons Flex imangosintha firmware ya gripper kuti ikhale yolumikizana ndi mtundu wa pulogalamu ya loboti. Zosintha za Gripper firmware nthawi zambiri zimakhala zachangu, ndipo zimachitika nthawi iliyonse:

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU
Mumangirira chogwirizira. Roboti imayambiranso.
Ngati, pazifukwa zilizonse, mtundu wanu wa gripper firmware ndi robotic software wasiya kulunzanitsa, mutha kusintha pamanja firmware mu Opentrons App.
1. Dinani Zida. 2. Dinani pa Flex yanu pamndandanda wa zida. 3. Pansi pa Zida ndi Ma modules, cholumikizira chakunja chidzawonetsa kuwerenga kwa banner yochenjeza.
"Zosintha za firmware zilipo." Dinani Update tsopano kuti muyambe kusintha.
Mutha view mtundu wa firmware womwe wakhazikitsidwa pano wa gripper. Pa touchscreen, pitani ku Zida ndikudina chogwirizira. Mu Opentrons App, pezani khadi yolumikizira pansi pa Zida ndi Ma module, dinani menyu ya madontho atatu (), kenako dinani About gripper.
3.4 Zoyimitsa Zadzidzidzi
Emergency Stop Pendant (E-stop) ndi batani lodzipatulira la hardware kuti muyimitse mofulumira kuyenda kwa robot. Opentrons Flex imafuna E-stop yogwira ntchito, yosasunthika kuti ikhale yolumikizidwa nthawi zonse. Mukakanikiza batani loyimitsa, Flex imaletsa ma protocol aliwonse omwe akuyenda kapena kukhazikitsa mayendedwe mwachangu momwe kungathekere ndikuletsa kuyenda kwa maloboti ambiri.
Nthawi yogwiritsira ntchito E-stop
Mungafunike kukanikiza E-stop:
Pakakhala ngozi yowopsa kapena kuvulazidwa kwa wogwiritsa ntchito. Pamene pali chiwopsezo choyandikira cha kuwonongeka kwa loboti kapena zida zina. Pamene sampochepera kapena ma reagents ali pachiwopsezo choyandikira kuipitsidwa. Pambuyo pa kugunda kwa hardware.
Moyenera simuyenera kukanikiza E-stop (kupatula pakuyesa kwamtundu wa Hardware).
Osagwiritsa ntchito E-stop kuletsa ntchito zanthawi zonse, zomwe zikuyembekezeka. M'malo mwake, gwiritsani ntchito batani la mapulogalamu pa touchscreen kapena Opentrons App. Kuyimitsa kudzera pa pulogalamu kukulolani kuti muyambirenso kapena kuletsa protocol yanu, pomwe kukanikiza E-stop nthawi zonse kumaletsa protocol nthawi yomweyo.

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU

Kuchita ndi kumasula E-stop
E-stop ili ndi makina osindikizira, opotoza kuti atulutse.
Gwirani ntchito: Kanikizani pansi mwamphamvu pa batani lofiira. Flex idzalowa m'malo oimitsidwa. Konzani: Mukayimitsidwa, yang'anani mosamala zovuta zilizonse pamalo ogwirira ntchito, monga kuchotsa zotayira,
kuchotsa labware, kapena kusuntha gantry (iyenera kuyenda momasuka komanso mosavuta ndi dzanja). Tulutsani: Jambulani batani molunjika. Idzatulukira pamalo ake osamangika. Bwezeraninso: Pa touchscreen kapena Opentrons App, tsimikizirani kuti mwakonzeka kuti Flex iyambirenso
kuyenda. Gantry ibwerera komwe idachokera ndipo ntchito ya module iyambiranso.
Poyimitsidwa, Flex ndi zida zolumikizidwa zizikhala motere:

Hardware Gantry Pipettes
Gripper
Heater-Shaker Module
Temperature Module Thermocycler Module Status light Touchscreen

Khalidwe
Kuyenda kopingasa zokha kwayimitsidwa. Kusuntha kwamanja kwamanja ndikololedwa.
Kuyenda moyima kwa ma pipette kwayimitsidwa. Mabuleki a injini pa nkhwangwa zoyima amamangidwa kuti apewe
pipettes kugwa. Kuyenda kwa plunger ndi kunyamula nsonga kwayimitsidwa.
Kuyenda koyima kwa chogwira kwayimitsidwa. The motor brake pa ofukula olamulira ndi chinkhoswe kupewa
chogwira kuti asagwe. Ma injini a nsagwada omwe amagwiritsa ntchito mphamvu yogwira amakhalabe othandizidwa, choncho
chogwira sichidzagwetsa labware chomwe chingakhale chonyamula.
Wogwedezayo amaima ndi nyumba. Latch ya labware imatsegulidwa. Kutentha kwazima.
Kutentha kapena kuziziritsa ndikozimitsa.
Kutentha kapena kuziziritsa ndikozimitsa.
Kuwala kumakhala kofiira.
Uthenga wolephereka umalowa pazenera. Chizindikiro chowonekera pazenera chikuwonetsa mukachita bwino
tsegulani batani loyimitsa.

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU
3.5 Zogwirizana

On/Off Switch

Zojambula Zam'mbali

Madoko a USB-A

IEC Power Inlet

Madoko AUX-1, AUX-2, USB-B, Efaneti

Kulumikizana kwamagetsi
Opentrons Flex imalumikizana ndi gwero lamagetsi kudzera panjira yolowera ya IEC-C14. Loboti ili ndi magetsi amkati a AC/DC, kuvomereza 100 VAC, kulowetsa kwa 240/50 Hz ndikusinthira kukhala 60 VDC. Zamagetsi zina zonse zamkati zimayendetsedwa ndi 36 VDC supply.

Chenjezo: Gwiritsani ntchito chingwe chamagetsi choperekedwa ndi loboti. Osagwiritsa ntchito chingwe chamagetsi chomwe chili ndi mphamvu yamagetsi yosakwanira kapena mphamvu yamagetsitagndi mavoti.
Sungani chingwe chamagetsi chopanda zotchinga kuti muchotse ngati kuli kofunikira.

Palinso batire ya CR1220 yachitsulo yopatsa mphamvu mawotchi anthawi yeniyeni a loboti ikakhala yosalumikizidwa ndi mphamvu zamagetsi. Batire ili mkati mwa mpanda wa touchscreen. Lumikizanani ndi Opentrons Support kuti mudziwe zambiri ngati mukuganiza kuti mukufunika kusintha batire.

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU
USB ndi maulumikizidwe othandizira
Opentrons Flex ili ndi madoko 10 onse a USB omwe ali m'malo osiyanasiyana a loboti, omwe amagwira ntchito zosiyanasiyana.
Madoko 8 akumbuyo a USB-A (omwe amatchedwa USB-1 kudzera pa USB-8) ndi madoko 2 othandizira (zolumikizira za M12 zolembedwa AUX-1 ndi AUX-2) ndi zolumikizira ma module ndi zida za Opentrons. Onani mutu wa Ma modules kuti mumve zambiri zolumikizira zidazi ndikuzigwiritsa ntchito pama protocol anu. Doko lakumbuyo la USB-B ndi lolumikizira loboti ku laputopu kapena kompyuta yapakompyuta, kukhazikitsa kulumikizana ndi Opentrons App yomwe ikuyenda pa kompyuta yolumikizidwa. Doko lakutsogolo la USB-A (USB-9), lomwe lili pansi pa chiwonetsero chazithunzi, lili ndi magwiridwe antchito ofanana ndi madoko akumbuyo a USB-A.
Chidziwitso: Madoko a USB ali ndi mphamvu zoteteza loboti ndi zida zolumikizidwa. Kutumiza mphamvu kumagawika mkati m'magulu atatu adoko: madoko akumanzere a USB-A (USB-1 kudzera pa USB-4), madoko akumanja a USB-A (USB-5 kudzera pa USB-8), ndi kutsogolo USB-A. doko. Lililonse la maguluwa lidzapereka 500 mA yochuluka kuzipangizo zogwirizana ndi USB 2.0.
Ma network
Opentrons Flex imatha kulumikizana ndi netiweki yapafupi ndi mawaya (Ethernet) kapena opanda zingwe (Wi-Fi).
Doko la Ethernet lili kumbuyo kwa loboti. Lumikizani ku Ethernet hub kapena sinthani netiweki yanu. Kapena, kuyambira mu mtundu wa robot 7.1.0, ilumikizeni mwachindunji ku doko la Efaneti pakompyuta yanu. Gawo lamkati la Wi-Fi limathandizira maukonde 802.11 ac/a/b/g/n okhala ndi mlongoti wa 2.4/5 GHz.

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU

3.6 Mafotokozedwe a dongosolo

General specifications

Miyeso Kulemera Sikelo mipata
Zenera logwira
Wi-Fi Efaneti USB
Kuyika kwamphamvu kwa roboti ya kamera
ZOYENERA KUTSATIRA |tage fluctuation Mains amapereka pafupipafupi kusinthasintha Dongosolo lagawidwe Kafupi kafupi kaphatikizidwe kamakono Kapangidwe kazenera Zofunikira pa mpweya wabwino

87 × 69 × 84 masentimita / 34.25 × 27 × 33 mu (W, D, H)
88.5 kg / 195 lb 12 ANSI / SLAS-mipata yogwirizana ndi malo ogwira ntchito
(zofikira ma pipette) 4 mipata yowonjezera ya stagmalangizo ndi labware
(chongofikira ku gripper) 7-inch LCD touchscreen yokhala ndi zokanda- komanso yosamva kuwonongeka kwa Gorilla Glass 3
802.11 ac/a/b/g/n dual-band (2.4/5 GHz)
100 Mbps 9 USB-A madoko 1 USB-B doko USB 2.0 liwiro
2MP, chithunzi ndi kanema 100 VAC, 240 Hz, 50 60 A/1 VAC, 4.0 A/115 VAC
±10%
±5%
TN-S
6.3 A
Chitsulo cholimba komanso kapangidwe ka aluminium CNC
Mawindo a mbali ya polycarbonate ndi khomo lakutsogolo Osachepera 20 cm / 8 pakati pa unit ndi khoma

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU

Zofunikira za PC zolumikizidwa

Opentrons App imagwira ntchito: Windows 10 kapena mtsogolo macOS 10.10 kapena kenako Ubuntu 12.04 kapena mtsogolo.

Mafotokozedwe a chilengedwe

Mikhalidwe ya chilengedwe Kutentha kozungulira Chinyezi chofananira Digiri ya kuipitsa

Kugwiritsa ntchito m'nyumba kokha +20 mpaka +25 °C (ndikovomerezeka) 40%, kusasunthika (kovomerezeka) 60 (kuipitsa kopanda conductive kokha)

Kuti mumve zambiri pazachilengedwe zovomerezeka zogwiritsidwa ntchito ndi zoyendera, onani gawo la Environmental Conditions pamutu wakuti Installation and Relocation.

Zitsimikizo

Ziphaso zatha Sizinatsimikizidwe/zovomerezeka

CE, ETL, FCC, ISO 9001 IVD, GMP

Chidule cha zidziwitso za certification chimasindikizidwa pa chomata kumbuyo kwa Flex, pafupi ndi cholumikizira / chozimitsa. Kuti mudziwe zambiri za certification ndi kutsata, onani gawo la Regulatory Compliance mu Mawu Oyamba.

Nambala ya siriyo
Flex iliyonse ili ndi nambala yake yapadera. Maonekedwe a serial nambala amapereka zambiri, kuphatikizapo tsiku lopangidwa ndi robot. Za example, nambala ya FLXA1020231007001 ingasonyeze:

Mtengo wa OPENTRONS FLEX

MUTU 3: KUFOTOKOZERA ZINTHU

Makhalidwe FLX A10 2023 10 07 001

Gulu la Model Version Chaka cha Mwezi wa Tsiku la Mwezi

Kutanthauza Loboti ndi Opentrons Flex. Khodi ya mtundu wa robot. Robotiyo idapangidwa mu 2023. Loboti idapangidwa mu Okutobala. Lobotiyo idapangidwa pa tsiku la 7 la mweziwo. Nambala yapadera ya maloboti opangidwa tsiku linalake.

Mutha kupeza nambala ya serial ya Flex yanu:
Pa chomata chotsimikizira kumbuyo kwa Flex, pafupi ndi cholumikizira / chozimitsa. Kumbali yakumbuyo ya touchscreen (kumalo ogwirira ntchito). Mu Opentrons App pansi pa Zida> Flex yanu> Zokonda za Robot> Zapamwamba.

Mtengo wa OPENTRONS FLEX

MUTU 4
Ma modules
Opentrons Flex imaphatikizana ndi ma module angapo a Opentrons hardware. Ma module onse ndi zotumphukira zomwe zimakhala m'malo otsetsereka, ndipo ambiri amawongoleredwa ndi loboti polumikizana ndi USB.
Mutuwu ukufotokoza ntchito ndi maonekedwe a thupi la ma modules omwe amagwirizana ndi Opentrons Flex system, komanso momwe angagwiritsire ntchito ndikuwongolera. Kuti mumve zambiri pakukhazikitsa ndi kugwiritsa ntchito ma module, onani zolemba zamagawo omwewo. Kuti mumve zambiri pakuphatikiza ma module mu ma protocol anu, onani gawo la Protocol Designer la mutu wa Protocol Development kapena zolemba za pa intaneti za Python Protocol API.
4.1 Ma module othandizira
Opentrons Flex imagwirizana ndi mitundu inayi ya ma module a Opentrons:
The Heater-Shaker Module imapereka kutentha padenga komanso kugwedezeka kwa orbital. Gawoli limatha kutentha mpaka 95 ° C, ndipo limatha kugwedeza sampkuchokera 200 mpaka 3000 rpm.
Magnetic Block ndi chipangizo chokhazikika chomwe chimasunga labware pafupi ndi maginito ake amphamvu kwambiri a neodymium. Ma OT-2 Magnetic Module GEN1 ndi GEN2, omwe amasuntha maginito awo m'mwamba ndi pansi poyerekeza ndi labware, sagwiritsidwa ntchito pa Opentrons Flex.
Temperature Module ndi gawo la mbale yotentha komanso yozizira yomwe imatha kusunga kutentha kwapakati pa 4 ndi 95 ° C.
Thermocycler Module imapereka padenga, makina opangira ma thermocycling, kupangitsa kuti masitepe oyenda kumtunda ndi kutsika ayendere. Thermocycler GEN2 imagwirizana kwathunthu ndi chophatikizira. Thermocycler GEN1 singagwiritsidwe ntchito ndi chophatikizira, chifukwa chake sichimathandizidwa pa Opentrons Flex.
Ma module ena omwe adapangidwira OT-2 amagwirizana ndi Flex, monga tafotokozera mwachidule patebulo pansipa. Chizindikiro chikuwonetsa kugwirizana, ndipo X ikuwonetsa kusagwirizana.

Mtengo wa OPENTRONS FLEX

MUTU 4: MAMODULE

Mtundu wa chipangizo ndi m'badwo wa Heater-Shaker Module GEN1 Magnetic Module GEN1 Magnetic Module GEN2 Magnetic Block GEN1 Temperature Module GEN1 Temperature Module GEN2 Thermocycler Module GEN1 Thermocycler Module GEN2 HEPA Module

OT-2

Flex

4.2 Module caddy system
Ma module ogwirizana adapangidwa kuti agwirizane ndi makadi omwe amakhala pansi pa sitimayo. Dongosololi limalola ma labware pamwamba pa ma module kuti akhalebe pafupi ndi pamwamba pa sitimayo, komanso amalola kuwongolera chingwe chapansi panthaka kotero kuti sitimayo imakhala yaudongo panthawi yomwe protocol yanu ikuyenda.

Ma Caddies a Heater-Shaker, Kutentha, ndi Ma module a Thermocycler.

Mtengo wa OPENTRONS FLEX

MUTU 4: MAMODULE
Kuti agwirizane ndi module pamwamba pa sitimayo, iyenera kuyikidwa kaye mu caddy yofananira. Mtundu uliwonse wa module yogwirizana uli ndi mapangidwe ake a caddy omwe amagwirizanitsa module ndi labware ndendende ndi sitima yozungulira. (Kupatulapo ndi Magnetic Block, omwe safuna mphamvu kapena USB chingwe routing ndipo motero amakhala molunjika pamwamba pamwamba.) Caddies kwa ma modules omwe amakhala ndi kagawo kamodzi akhoza kuikidwa paliponse mu ndime 1 kapena 3; Thermocycler ikhoza kuikidwa mu mipata A1 ndi B1 nthawi imodzi.
Nthawi zambiri, kukhazikitsa module caddy:
1. Chotsani mipata iliyonse pamalo pomwe gawoli lipita. 2. Ikani gawolo mu caddy yake ndikumangitsa anangula ake. 3. Yendetsani mphamvu ya module ndi zingwe za USB kudzera pazivundikiro zam'mbali, kupita kumtunda wopanda kanthu, ndi
agwirizane nawo ku module. 4. Khazikitsani module caddy mu kagawo ndikuyipukuta m'malo mwake.
Kuti mupeze malangizo enieni oyika, onani Quickstart Guide kapena Instruction Manual pagawo linalake. Malumikizidwe a chingwe ndi njira yolumikizira ku caddy amasiyana ndi module.
4.3 Kusintha kwa ma module
Mukayamba kukhazikitsa gawo pa Flex, muyenera kuyendetsa ma calibration okhazikika. Njirayi ndi yofanana ndi kuwerengetsera kwa zida, ndikuwonetsetsa kuti Flex imasunthira kumalo olondola kuti agwire bwino ntchito. Pakuwongolera, Flex imasunthira kumalo omwe ali pa adapter calibration calibration, yomwe imawoneka yofanana ndi mabwalo owerengera omwe ali mbali ya mipata yochotsamo.

Ma adapter a Calibration a Heater-Shaker, Temperature, ndi Thermocycler Modules.
Kuwongolera ma module ndikofunikira pama module onse omwe amayika kudzera pa caddy: Heater-Shaker, Temperature, ndi Thermocycler Modules. Magnetic Block safuna kusinthidwa, ndipo ndi okonzeka kugwiritsidwa ntchito mukangoyiyika pa sitimayo.

Mtengo wa OPENTRONS FLEX

MUTU 4: MAMODULE
Nthawi yosinthira ma module
Flex imakupangitsani kuti musamalire mukalumikiza ndikuyatsa gawo lomwe lilibe data yosungira yosungidwa. (Mutha kuletsa izi, koma simungathe kuyendetsa ma protocol ndi module mpaka mutayiyesa.)
Mukamaliza kuwongolera, Flex imasunga data yoyeserera ndi nambala ya serial kuti igwiritsidwe ntchito mtsogolo. Flex sidzakupangitsani kuti musinthenso pokhapokha mutachotsa deta ya calibration ya gawolo muzokonda za robot. Mutha kuyimitsa momasuka gawo lanu ndikuyimitsa, kapena kuyisuntha kupita kumalo ena, osafunikira kukonzanso. Ngati mukufuna kukonzanso, mutha kuyambitsa ndondomekoyi nthawi iliyonse kuchokera pa module card mu Opentrons App. (Kukonzanso sikupezeka pa touchscreen.)
Momwe mungasankhire ma module
Malangizo pa touchscreen kapena Opentrons App adzakuwongolerani momwe mungasinthire. Nthawi zambiri masitepe ndi:
1. Sonkhanitsani zida zofunika, kuphatikizapo adaputala ya module calibration ndi pipette calibration probe. 2. Ikani adaputala ya calibration pamwamba pa module ndikuwonetsetsa kuti ndiyonse.
Ma module ena angafunike kuti mumange adaputala ku module. 3. Gwirizanitsani kafukufuku wa calibration ku pipette. 4. Flex imangosuntha kuti ikhudze mfundo zina pa adaputala yosinthira ndikusunga izi
ma calibration kuti agwiritsidwe ntchito mtsogolo.
Kuwongolera kukatha ndipo mwachotsa adaputala ndi kufufuza, gawoli likhala lokonzeka kugwiritsidwa ntchito pama protocol.
Nthawi iliyonse, mungathe view ndikuwongolera data ya ma module anu mu Opentrons App. Pitani ku Zikhazikiko za Robot za Flex yanu ndikudina pa Calibration tabu.

Mtengo wa OPENTRONS FLEX

MUTU 4: MAMODULE
4.4 Heater-Shaker Module GEN1

Mawonekedwe a Heater-Shaker
KUTENGA NDI KUTENGA
The Heater-Shaker imapereka kutentha kwapadenga komanso kugwedezeka kwa orbital. Gawoli limatha kutenthedwa mpaka 95 ° C, ndikutsatira kutentha kwa profile:
Kutentha osiyanasiyana: 37 °C Kutentha kolondola: ± 95 °C pa 0.5 °C Kutentha mofanana: ± 55 °C pa 0.5 °CRamp mlingo: 10 °C / min
Module imatha kugwedeza sampkutsika kuchokera ku 200 mpaka 3000 rpm, ndi zotsatirazi zogwedezafile:
Kutalika kwa orbital: 2.0 mm Mayendedwe a Orbital: Kuthamanga kwa Clockwise: 200 rpm Kuthamanga kwachangu: ± 3000 rpm
Module ili ndi latch labware yotetezedwa kuti iteteze mbale ku gawoli musanagwedezeke.

Mtengo wa OPENTRONS FLEX

MUTU 4: MAMODULE
MA ADAPTER OTCHULUKITSA Adapter yogwirizana ndiyofunikira kuti muwonjezere labware ku Heater-Shaker. Adapter zitha kugulidwa mwachindunji kuchokera ku Opentrons pa https://shop.opentrons.com. Ma Adapter a Thermal omwe alipo pano akuphatikizapo:

Adapter ya Universal Flat

Adapter ya PCR

Adapter ya Deep Well

96 Flat Pansi Adapter

MALANGIZO A SOFTWARE
The Heater-Shaker imatha kukonzedwa bwino mu Protocol Designer ndi Python Protocol API. Python API imalolanso kuti masitepe ena a protocol achitidwe mofanana pomwe Heater-Shaker ikugwira ntchito. Onani malamulo Osatsekereza muzolemba za API kuti mumve zambiri pakuwonjezera njira zofananira ndi ma protocol anu.
Kunja kwa ma protocol, Opentrons App imatha kuwonetsa momwe Heater-Shaker ilili ndipo imatha kuwongolera mwachindunji chowotcha, shaker, ndi latch labware.

Mtengo wa OPENTRONS FLEX

MUTU 4: MAMODULE

Zotsatira za Heater-Shaker

Dimensions Kulemera kwa module yolowetsa mphamvu ya adaputala Mphamvu ya adaputala Mains supply voltagndi kusinthasintha kwa Overvoltage Kugwiritsa ntchito mphamvu

152 × 90 × 82 mm (L/W/H) 1.34 kg 36 VDC, 6.1 A 100 VAC, 240/50 Hz ± 60% Category II Idle: 10 W

Chitsanzo: Kugwedeza: 4 W Kutentha: 11 W Kutentha ndi kugwedezeka: 10 W

Mikhalidwe ya chilengedwe Kutentha kozungulira Chinyezi chofanana ndi kutalika kwake Digiri ya kuipitsa

Kuchuluka: 125 W Kugwiritsira ntchito m'nyumba 130 °C kokha Kufikira 20%, osasunthika Kufikira 25 m pamwamba pa nyanja 80

Mtengo wa OPENTRONS FLEX

MUTU 4: MAMODULE
4.5 Maginito Block GEN1

Mawonekedwe a Magnetic Block
Opentrons Magnetic Block GEN1 ndi chonyamula maginito 96-chitsime. Maginito a maginito amagwiritsidwa ntchito m'ma protocol omwe amadalira maginito kuti atulutse tinthu ting'onoting'ono kuti tiyimitsidwe ndikuzisunga m'mbale zapachitsime panthawi yochapa, kutsuka, kapena njira zina zowonongeka. Za example, kukonzekera NGS yodzichitira; kuyeretsa DNA ya genomic ndi mitochondrial, RNA, kapena mapuloteni; ndi njira zina zochotsera zonse ndizogwiritsa ntchito zomwe zingakhudze maginito.
ZINTHU ZA MAGNETIC
Magnetic Block ilibe mphamvu, ilibe zida zilizonse zamagetsi, ndipo samasuntha mikanda ya maginito mmwamba kapena pansi mu yankho. Zitsimezi zimakhala ndi maginito 96 amphamvu kwambiri a neodymium omwe amaikidwa pa bedi lodzaza ndi masika, zomwe zimathandiza kuti pakhale kulolerana pakati pa chipika ndi ma pipette pamene mukuyendetsa ma protocol odzichitira.
MALANGIZO A SOFTWARE
Magnetic Block GEN1 ndi yotheka kukonzedwa mu Protocol Designer ndi Python Protocol API.

Mtengo wa OPENTRONS FLEX

MUTU 4: MAMODULE

Kunja kwa ma protocol, komabe, chotchingira ndi Opentrons App sadziwa ndipo sangathe kuwonetsa momwe Magnetic Block GEN1 ilipo. Iyi ndi module yopanda mphamvu. Zilibe zida zamagetsi kapena zamakina zomwe zimatha kulumikizana ndi loboti ya Flex. "Mumawongolera" Maginito Block kudzera mu ndondomeko zomwe zimagwiritsa ntchito Opentrons Flex Gripper kuwonjezera ndi kuchotsa labure mu gawoli.

Mafotokozedwe a Magnetic Block

Makulidwe Kunenepa Module mphamvu Maginito giredi Mikhalidwe ya chilengedwe Kutentha kozungulira Chinyezi chofananira Mulingo Digiri ya kuipitsa

136 × 94 × 45 mamilimita (L/W/H) 1.13 makilogalamu Palibe, gawo ndi opanda mphamvu N52 neodymium M'nyumba ntchito kokha 20 °C 25%, sanali condensing Mpaka 30 mamita pamwamba pa nyanja 80

Mtengo wa OPENTRONS FLEX

MUTU 4: MAMODULE
4.6 Kutentha Module GEN2

Kutentha kwa module
KUCHENGA NDI KUZIGIRIRA
Opentrons Temperature Module GEN2 ndi gawo lotentha komanso lozizira. Nthawi zambiri amagwiritsidwa ntchito pama protocol omwe amafunikira kutentha, kuziziritsa, kapena kusintha kwa kutentha. Gawoli limatha kufikira ndikusunga kutentha kuyambira 4 ° C mpaka 95 ° C mkati mwa mphindi, kutengera kasinthidwe ndi zomwe zili mkati mwake.
MABUKU OTSATIRA
Kuti agwire labware kutentha, gawoli limagwiritsa ntchito midadada yotentha ya aluminium. Module imabwera ndi 24well ndi 96-chabwino matenthedwe midadada. The Temperature Module caddy imabwera ndi chipika chakuya komanso chotchinga pansi chopangidwa kuti chigwiritsidwe ntchito ndi Flex Gripper. Mipiringidzo imakhala ndi machubu a 1.5 mL ndi 2.0 mL, mbale za PCR za 96-well, zingwe za PCR, mbale zakuya, ndi mbale zapansi.

Mtengo wa OPENTRONS FLEX

MUTU 4: MAMODULE
Zindikirani: Gawoli limatumizanso ndi chipika chapansi cha OT-2. Osagwiritsa ntchito block ya OT-2 ndi Flex. Chophimba chapansi cha Flex chili ndi mawu akuti "Opentrons Flex" pamwamba pake. Mmodzi wa OT-2 alibe.

24-chitsime chotentha chamafuta

96-chitsime chotentha chamafuta

Kuzama bwino matenthedwe chipika

Malo otentha apansi a Flex

KUSAMBIRA KWA MADZI NDI KUTENGETSA
Chifukwa mpweya ndi insulator yabwino yotentha, mipata pakati pa labware ndi chotchinga chotenthetsera imatha kusokoneza magwiridwe antchito a Temperature Module. Kuyika madzi pang'ono muzitsulo zotentha za 24- kapena 96 kumachepetsa mipata ya mpweya ndikuwongolera kutentha kwabwino. Kuchuluka kwa madzi kumadalira chipika chamafuta ndi labware. Onani Temperature Module White Paper kuti mumve zambiri.

MALANGIZO A SOFTWARE
The Temperature Module imatha kukonzedwa bwino mu Protocol Designer ndi Python Protocol API.
Kunja kwa ma protocol, Opentrons App imatha kuwonetsa momwe zilili pano pa Temperature Module ndipo imatha kuwongolera kutentha kwa mbale.

Mtengo wa OPENTRONS FLEX

MUTU 4: MAMODULE

Kutentha kwa Module

Miyeso Kulemera Module mphamvu
Mikhalidwe ya chilengedwe Kutentha kozungulira Chinyezi chofanana ndi kutalika kwake Digiri ya kuipitsa

194 × 90 × 84 mm (L/W/H) 1.5 kg Zolowetsa: 100 VAC, 240/50 Hz, 60 A Kutulutsa: 4.0 VDC, 36 A, 6.1 W max Kugwiritsa ntchito m'nyumba kokha <219.6 °C (kuzizira koyenera ndi kokwanira Kufikira 22%, osasunthika Kufikira 60 m pamwamba pa nyanja 2000

4.7 Thermocycler Module GEN2

Mtengo wa OPENTRONS FLEX

MUTU 4: MAMODULE
Zochita za Thermocycler
Opentrons Thermocycler Module GEN2 ndi chotenthetsera chomwe chili padenga, chopereka PCR yopanda manja mumtundu wa mbale 96. Chivundikiro chake chotenthedwa ndi chosindikizira chotayidwa chimakwanira bwino pa mbale, kuwonetsetsa kuti s yogwira ntchito bwinoample Kutentha ndi evaporation pang'ono.
KUTENGA NDI KUZIRIRA Chotchinga cha Thermocycler chimatha kutentha ndi kuzizira, ndipo chivindikiro chake chimatha kutentha, ndi kutentha kotsatiraku.file: Kutentha kwa block block: 4 °C Thermal block pazipita Kutentha ramp mlingo: 4.25 °C/s kuchokera ku GEN2 yozungulira mpaka 95 °C Thermal block pazipita kuzizirira ramp mlingo: 2.0 °C/s kuchokera 95 °C mpaka yozungulira Lid kutentha osiyanasiyana: 37 °C chivindikiro kutentha molondola: ± 110 °C Chivundikiro chodzichitira okha akhoza kutsegulidwa kapena kutsekedwa pakufunika pakuchita protocol.
Mtengo wa magawo THERMOCYCLER PROFILES The Thermocycler imatha kuchita profiles: kumangoyenda panjinga motsatizana ndi kutentha kwa chipika kuti muchite zinthu zosagwirizana ndi kutentha.
ZIZINDIKIRO ZA RUBBER AUTOMATION Thermocycler imabwera ndi zisindikizo za raba automation kuti zithandizire kuchepetsa kutuluka kwa nthunzi. Chisindikizo chilichonse chiyenera kutsekedwa chisanagwiritsidwe ntchito ndipo chingagwiritsidwe ntchito maulendo angapo. Zisindikizo zowonjezera zitha kugulidwa mwachindunji kuchokera ku Opentrons pa https://shop.opentrons.com.
KULAMULIRA KWA SOFTWARE Thermocycler imatha kukonzedwa bwino mu Protocol Designer ndi Python Protocol API. Kunja kwa ma protocol, Opentrons App imatha kuwonetsa momwe Thermocycler ilili ndipo imatha kuwongolera kutentha kwa chipika, kutentha kwa chivindikiro, ndi malo a chivindikiro.

Mtengo wa OPENTRONS FLEX

MUTU 4: MAMODULE

Thermocycler specifications

Makulidwe (chivundikiro chotseguka) Makulidwe (chivundikiro chatsekedwa) Kulemera (kuphatikiza njira yakumbuyo) Mphamvu ya adapter vol.tage Power adaputala panopa Overvoltage Mikhalidwe ya chilengedwe Kutentha kozungulira Chinyezi chofanana ndi kutalika kwake Zofunikira pa mpweya wabwino

244.95 × 172 × 310.1 mm (L/W/H) 244.95 × 172 × 170.35 mm (L/W/H) 8.4 kg 100 V pa 240/50 Hz 60 A Category II Kugwiritsa ntchito m’nyumba 8.5 ° C5 kokha 20 ° C (chovomerezeka) 25%, chosasunthika Kufikira 2 m pamwamba pa nyanja Osachepera 40 cm / 30 pakati pa unit ndi khoma

Mtengo wa OPENTRONS FLEX

MUTU 5
Labware
Mutuwu ukukhudza zinthu zomwe zili mu Laibulale ya Opentrons Labware yomwe mungagwiritse ntchito ndi Opentrons Flex ndi Opentrons Flex Gripper. Ikuphatikizanso ma labware ndipo, kwa ogwiritsa ntchito mphamvu, imalumikiza zida za labware ku JSON yawo yofananira. file matanthauzo.
Mutha kugula labware kuchokera kwa opanga zida zoyambira kapena kusitolo ya Opentrons pa https://shop.opentrons.com. Ndipo, Opentrons imagwira ntchito nthawi zonse kuti itsimikizire matanthauzidwe atsopano a labware. Onani Laibulale ya Labware (yolumikizidwa pamwambapa) kuti mupeze mindandanda yaposachedwa.
5.1 Malingaliro a Labware
Labware imaphatikizapo zambiri kuposa zinthu zomwe zimayikidwa pamtunda ndikugwiritsidwa ntchito mu protocol. Kukuthandizani kumvetsetsa labware ya Opentrons, tiyeni tiwunikenso mutuwu kuchokera munjira zitatu zosiyanasiyana. Kwa Opentrons Flex, labware imaphatikizapo zinthu zomwe zili mu Laibulale yathu ya Labware, deta yomwe imatanthawuza chidutswa chilichonse cha labware, ndi labware makonda.
Labure ngati hardware
Laibulale ya Labware imaphatikizapo chilichonse chomwe mungagwiritse ntchito mwachisawawa ndi Opentrons Flex. Izi ndi zinthu zolimba komanso zotha kudyedwa zomwe mumagwiritsa ntchito, kugwiritsanso ntchito, kapena kutaya mukamagwiritsa ntchito protocol. Simufunikanso kuchitapo kanthu kuti mugwire ntchito ndi zinthu zomwe zili mu Labware Library. Loboti ya Flex imadziwa kugwira ntchito ndi chilichonse chomwe chili mulaibulale yokha.
Labware ngati data
Zambiri zamalabu zimasungidwa mu Javascript object notation (JSON) filendi .json file zowonjezera. A JSON file imaphatikizapo miyeso ya malo (utali, m'lifupi, kutalika), mphamvu ya volumetric (L, mL), ndi ma metrics ena omwe amatanthauzira mawonekedwe a pamwamba, mawonekedwe awo, ndi malo. Mukamagwiritsa ntchito protocol, Flex imawerenga izi .json filendikudziwa kuti labware yomwe ili pamtunda ndi momwe mungagwirire nayo ntchito.

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE
Custom labware
Custom Labware ndi labware yomwe ilibe mu Labware Library kapena ndi labware yopangidwa ndi Custom Labware Creator. Komabe, nthawi zina lingaliro lazolemba zamakalata limalemedwa ndi malingaliro azovuta, ndalama, kapena zovuta. Koma, labware makonda sikuyenera kukhala kovuta kumvetsetsa kapena kupanga. Tiyeni titenge kamphindi kuti titulutse lingaliro la labware yokhazikika.
Monga exampLero, Laibulale ya Opentrons Labware ili ndi mbale za 96 (200 L) zochokera ku Corning ndi BioRad, koma opanga ena amapanganso mbale izi. Ndipo, chifukwa cha miyezo yovomerezeka yamakampani, kusiyana pakati pa zinthu za labu zomwe zimapezeka paliponse ndizochepa. Komabe, mbale wamba ya 200 L, 96-chitsime yochokera ku Stellar Scientific, Oxford Lab, kapena Krackeler Scientific (kapena wopereka wina aliyense pankhaniyi) ndi "custom labware" ya Flex chifukwa sichinafotokozedwe mu Labware Library yathu. . Kuphatikiza apo, kusiyana kwakung'ono mumiyeso ya labware kumatha kukhudza kwambiri kupambana kwa protocol yanu. Pachifukwa ichi, ndikofunikira kukhala ndi tanthauzo lolondola la labware pa labu iliyonse yomwe mukufuna kugwiritsa ntchito mu protocol yanu.
Komanso, ngakhale ma labware achikhalidwe amatha kukhala a esoteric, chidutswa chimodzi cha zida, nthawi zambiri amakhala nsonga, mbale, machubu, ndi zoyika zomwe zimagwiritsidwa ntchito tsiku lililonse m'ma lab padziko lonse lapansi. Apanso, kusiyana kokha pakati pa Opentrons labware ndi labware makonda ndi chinthu chatsopanocho sichinatchulidwetu mu pulogalamu yomwe imapatsa mphamvu loboti. The Flex imatha, ndipo imagwira ntchito ndi zinthu zina zoyambira labware kapena china chake chapadera, koma muyenera kulemba mawonekedwe a chinthucho mu tanthauzo la labware JSON. file ndikulowetsa datayo mu Opentrons App. Onani gawo la Custom Labware Definitions pansipa kuti mudziwe zambiri.
Pomaliza, labware imaphatikizapo:
Chilichonse mu Opentrons Labware Library. Matanthauzo a Labware: data mu JSON file zomwe zimatanthawuza mawonekedwe, makulidwe, ndi kuthekera kwa zinthu payekha
monga mbale mbale, nsonga, nkhokwe, etc. Mwambo labware, amene ali zinthu zimene sizinaphatikizidwe mu Labware Library.
Pambuyo pa reviewPoganizira mfundo zofunika zimenezi, tiyeni tione magulu ndi zinthu mu Opentrons Labware Library. Pambuyo pake, timamaliza mutuwo ndikumalizaview za zigawo za data za labware file ndi kufotokoza mwachidule mawonekedwe a Opentrons ndi ntchito zomwe zimakuthandizani kupanga labware yokhazikika.

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE

5.2 Madamu
Opentrons Flex imagwira ntchito mwachisawawa ndi malo okhala ndi zitsime zambiri zomwe zalembedwa pansipa. Kugwiritsa ntchito ma reservoirs awa kumathandizira kuchepetsa ntchito yanu yokonzekera chifukwa ndi okonzeka kutuluka m'bokosi. Zambiri zosungiramo madzi zimapezekanso mu Opentrons Labware Library.

Malo osungiramo chitsime chimodzi

Zolemba Zopanga

Wanzeru

290 mL V pansi

Axygen

90 ml ya madzi pansi

NEST

195 ml ya madzi pansi

290 mL V pansi

Dzina lotsegula la API
agilient_1_ reservoir_290ml
mpweya_1_ posungira_90ml
nest_1_ reservoir_195ml
nest_1_ reservoir_290ml

Multi-well reservoirs

Zolemba Zopanga

NEST

12 zitsime 15 mL / bwino V pansi

USA Sayansi

12 zitsime 22 mL / bwino V pansi

API katundu dzina nest_12_ reservoir_15ml
usascientific_12_reservoir_22ml

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE
Reservoirs ndi matanthauzo a API
Laibulale ya Opentrons Labware imatanthawuza mawonekedwe a malo osungira omwe alembedwa pamwambapa mu JSON yosiyana files. Loboti ndi Opentrons Python API zimadalira matanthauzidwe awa a JSON kuti azigwira ntchito ndi labware yogwiritsidwa ntchito ndi ma protocol anu. Za example, pogwira ntchito ndi API, ntchito ya ProtocolContext.load_labware imavomereza mayina a labware awa ngati magawo ovomerezeka mu code yanu. Maina olemetsa a API olumikizidwa amalumikizana ndi matanthauzo a labware ya reservoir munkhokwe ya Opentrons GitHub.
Mwambo reservoir labware
Yesani kupanga matanthauzidwe a labware ndi Opentrons Labware Creator ngati chosungira chomwe mukufuna kugwiritsa ntchito sichinalembedwe apa. Kutanthauzira kwachizolowezi kumaphatikiza miyeso yonse, metadata, mawonekedwe, kuchuluka kwa volumetric, ndi zina zambiri mu JSON. file. Opentrons Flex ikufunika izi kuti imvetsetse momwe mungagwirire ntchito ndi labure yanu. Onani gawo la Custom Labware Definitions kuti mudziwe zambiri.
5.3 Zitsime zosungira
Opentrons Flex imagwira ntchito mwachisawawa ndi mbale zomwe zalembedwa pansipa. Kugwiritsa ntchito mbale izi kumathandizira kuchepetsa ntchito yokonzekera kukonzekera chifukwa ndi okonzeka kutulutsa. Zambiri za mbale zikupezekanso mu Opentrons Labware Library.

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE

6-chitsime mbale
Wopanga Corning

Zofotokozera
6 zitsime 16.8 mL / chitsime Zozungulira zitsime, pansi lathyathyathya

API katundu dzina corning_6_wellplate_16.8ml_flat

12-chitsime mbale
Wopanga Corning

Zofotokozera
12 zitsime 6.9 mL / chitsime Zozungulira zitsime, pansi lathyathyathya

API katundu dzina corning_12_wellplate_6.9ml_flat

24-chitsime mbale
Wopanga Corning

Zofotokozera
24 zitsime 3.4 mL / chitsime Zozungulira zitsime, pansi lathyathyathya

API katundu dzina corning_24_wellplate_3.4ml_flat

48-chitsime mbale
Wopanga Corning

Zofotokozera
48 zitsime 1.6 mL / chitsime Zozungulira zitsime, pansi lathyathyathya

API katundu dzina corning_48_wellplate_1.6ml_flat

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE

96-chitsime mbale
Wopanga Bio-Rad Corning NEST
Opentrons Thermo Scientific
USA Sayansi

Zofotokozera
96 zitsime 200 µL/chitsime Zitsime zozungulira, V pansi
96 zitsime 360 µL/chitsime zozungulira zitsime, pansi lathyathyathya
96 zitsime 100 µL/chitsime Zozungulira zitsime, V pansi PCR siketi yodzaza
96 zitsime 200 µL/chitsime zozungulira zitsime, pansi lathyathyathya
96 zitsime zakuya 2000 µL / bwino Square zitsime, V pansi
Zitsime zolimba 96 200 µL / zitsime zozungulira, V pansi PCR siketi yonse
Zitsime zakuya za Nunc 96 1300 µL/zitsime zozungulira, U pansi
Zitsime zakuya za Nunc 96 2000 µL/zitsime zozungulira, U pansi
96 zitsime zakuya 2.4 mL / chitsime Square zitsime, U pansi

API katundu dzina biorad_96_wellplate_200ul_pcr
corning_96_wellplate_360ul_flat
nest_96_wellplate_100ul_pcr_full_skirt
nest_96_wellplate_200ul_flat
nest_96_wellplate_2ml_deep
opentrons_96_wellplate_200ul_pcr_full_ siketi
thermoscientificnunc_96_wellplate_ 1300ul thermoscientificnunc_96_wellplate_ 2000ul usascientific_96_wellplate_2.4ml_deep

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE

384-chitsime mbale

Manufacturer Applied Biosystems Bio-Rad
Corning

Zofotokozera
384 zitsime 40 µL/chitsime Zitsime zozungulira, V pansi
384 zitsime 50 µL/chitsime Zitsime zozungulira, V pansi
384 zitsime 112 µL/chitsime Square zitsime, pansi lathyathyathya

API katundu dzina appliedbiosystemsmicroamp_384_ wellplate_40ul biorad_384_wellplate_50ul
corning_384_wellplate_112ul_flat

Ma adapter mbale bwino
Ma mbale a aluminiyamu omwe alembedwa pansipa ndi ma adapter otentha a module ya Opentrons Heater-Shaker GEN1. Mutha kugwiritsa ntchito matanthauzidwe a adaputala awa kuti muyike Opentrons yotsimikizika kapena labure yokhazikika pamwamba pa Heater-Shaker.

Adapter mtundu wa Opentrons Universal Flat Heater-Shaker Adapter Opentrons 96 PCR Heater-Shaker Adapter Opentrons 96 Deep Well Heater-Shaker Adapter Opentrons 96 Flat Bottom Heater-Shaker Adapter

API katundu dzina opentrons_universal_flat_adapter opentrons_96_pcr_adapter opentrons_96_deep_well_adapter opentrons_96_flat_bottom_adapter

Mukhozanso kutsegula adaputala ndi labware ndi tanthauzo limodzi. Laibulale yathu ya Labware imaphatikizapo ma adapter angapo otenthetsera omwe adasinthidwa kale ndi ma labware omwe amapangitsa Heater-Shaker kukhala yokonzeka kugwiritsa ntchito kunja kwa bokosilo.

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE

Chidziwitso: Osagwiritsa ntchito matanthauzidwe ophatikizika ngati mukufuna kusuntha labware pa Heater-Shaker panthawi ya protocol yanu, kaya ndi gripper kapena pamanja. Gwiritsani ntchito matanthauzo a adaputala odziyimira m'malo mwake.

Adapter / labware kuphatikiza

Dzina lotsegula la API

Opentrons 96 Deep Well Heater-Shaker Adapter yokhala ndi NEST Deep Well Plate 2 ml

opentrons_96_deep_well_adapter_nest_ wellplate_2ml_deep

Opentrons 96 Flat Bottom Heater-Shaker Adapter yokhala ndi NEST 96 Well Plate 200 µL Flat

opentrons_96_flat_bottom_adapter_nest_ wellplate_200ul_flat

Adapter ya Opentrons 96 PCR Heater-Shaker yokhala ndi NEST Well Plate 100 µL

opentrons_96_pcr_adapter_nest_wellplate_ 100ul_pcr_full_skirt

Adapter ya Opentrons Universal Flat Heater-Shaker yokhala ndi Corning 384 Well Plate 112 µL Flat

opentrons_universal_flat_adapter_corning_384_ wellplate_112ul_flat

Adapter zitha kugulidwa mwachindunji kuchokera ku Opentrons pa https://shop.opentrons.com.

Ma plates abwino ndi matanthauzo a API
Laibulale ya Opentrons Labware imatanthauzira mawonekedwe a mbale zachitsime zomwe zalembedwa pamwambapa mu JSON files. Loboti ya Flex ndi Opentrons Python API imadalira matanthauzo a JSON kuti agwire ntchito ndi labware yogwiritsidwa ntchito ndi ma protocol anu. Za example, pogwira ntchito ndi API, ntchito ya ProtocolContext.load_labware imavomereza mayina a labware awa ngati magawo ovomerezeka mu code yanu. Mayina okhudzana ndi katundu wa API amalumikizana ndi matanthauzo a labware ya chitsime munkhokwe ya Opentrons GitHub.

Mwambo bwino mbale labware
Yesani kugwiritsa ntchito Opentrons Labware Creator kuti mupange tanthauzo la labware ngati chitsime chomwe mukufuna kugwiritsa ntchito sichinalembedwe apa. Kutanthauzira kwachizolowezi kumaphatikiza miyeso yonse, metadata, mawonekedwe, kuchuluka kwa volumetric, ndi zina zambiri mu JSON. file. Opentrons Flex imawerenga izi kuti mumvetsetse momwe mungagwirire ntchito ndi labure yanu. Onani gawo la Custom Labware Definitions kuti mudziwe zambiri.

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE

5.4 Malangizo ndi zida zopangira
Malangizo a Opentrons Flex amabwera mu makulidwe a 50 µL, 200 µL, ndi 1000 μL. Awa ndi malangizo omveka bwino, osayendetsa polypropylene omwe amapezeka ndi zosefera kapena zopanda. Amapakidwa osabala m'marack omwe amakhala ndi nsonga 96 ndipo alibe DNase, RNase, protease, pyrogens, DNA yamunthu, endotoxins, ndi PCR inhibitors. Ma Racks amaphatikizanso nambala zambiri komanso masiku otha ntchito.
Malangizo a ma pipette a Flex amagwira ntchito ndi ma pipette a Opentrons Flex 50 µL ndi 1000 µL mu 1-, 8-, ndi 96-channel. Ngakhale mutha kuyika malangizo aliwonse a Flex pa 50 L ndi 1000 L pipettes, yesani kufananiza nsonga ndi kuchuluka kwa pipette. Za exampLero, zingakhale zosamvetseka kuyika nsonga ya 1000 L pa 50 L pipette. Pa 1000 L pipette, mutha kugwiritsa ntchito nsonga ya 50 L, 200 L, kapena 1000 L.

Zopangira nsonga
Nsonga zosasefedwa ndi zosefedwa zimakulungidwa muchoyikapo chomwe chimakhala ndi mbale yoyambira yogwiritsidwanso ntchito, mbale yapakatikati yomwe imakhala ndi nsonga 96, ndi chivindikiro.

Tip rack ndi voliyumu 50 µL 200 µL 1000 µL

Dzina lotsegula la API
Zosasefedwa: opentrons_flex_96_tiprack_50ul Zosefedwa: opentrons_flex_96_filtertiprack_50ul
Zosasefedwa: opentrons_flex_96_tiprack_200ul Zosefedwa: opentrons_flex_96_filtertiprack_200ul
Zosasefedwa: opentrons_flex_96_tiprack_1000ul Zosefedwa: opentrons_flex_96_filtertiprack_1000ul

Kuti zithandizire kuzizindikiritsa, mbale zapakati pa nsonga zokhala ndi utoto zimatengera kukula kwa nsonga:
50 µL: magenta 200 L: yellow 1000 µL: buluu

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE

Mukayitanitsa kapena kuyitanitsanso, maupangiri ndi ma racks amabwera m'mapangidwe awiri osiyana:
Ma Racks: Amakhala ndi nsonga zokutidwa padera (mbale, mbale yapakati yokhala ndi nsonga, ndi chivindikiro). Masinthidwe okhetsedwa amakhala abwino kwambiri ngati ukhondo uli wofunika kwambiri, kupewa kuipitsidwa, kapena ngati ma protocol anu sakulola kuti mbale kapena chigawo chanu chigwiritsidwenso ntchito.
Zowonjezeredwa: Zimakhala ndi nsonga imodzi yathunthu (mbale yoyambira, mbale yapakati yokhala ndi nsonga, ndi chivindikiro) ndi zotengera zamomwemo. Zosintha zodzazanso zimakhala zabwino kwambiri ngati ma protocol anu amalola kuti mbale yapansi igwiritsidwenso ntchito.
Kugwirizana kwa Tippipette
Malangizo a pipette a Flex adapangidwira ma pipette a Opentrons Flex. Malangizo a Flex samabwerera m'mbuyo ogwirizana ndi Opentrons OT-2 pipettes, komanso simungagwiritse ntchito malangizo a OT-2 pa Flex pipettes.
Maupangiri ena amakampani amatha kugwira ntchito ndi Flex pipettes, koma izi sizovomerezeka. Kuti muwonetsetse kuti mukugwira ntchito bwino, muyenera kugwiritsa ntchito malangizo a Opentrons Flex okhala ndi Flex pipettes.
Adaputala yoyambira
Pipette ya 96-channel imafuna adaputala kuti imangirire nsonga zonse bwino. Panthawi yophatikizira, pipette imayenda pamwamba pa adaputala, imatsikira pazikhomo, ndikukokera nsonga pazipiipi pokweza adaputala ndi nsonga choyikapo.

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE

Chidziwitso: Ingogwiritsani ntchito adaputala yoyika nsonga mukatolera nsonga zonse nthawi imodzi. Ikani nsonga zotchingira pa sitimayo potenga nsonga zochepa.
Chenjezo: Chiwopsezo cha kutsina. Sungani manja kutali ndi adaputala ya nsonga pamene pipette ikugwirizanitsa nsonga za pipette.

Adapter yamtundu wa Opentrons Flex 96 Tip Rack Adapter

API katundu dzina opentrons_flex_96_tiprack_adapter

Adaputala yopangira nsonga imagwirizana ndi Opentrons Flex Gripper. Mutha kugwiritsa ntchito chophatikizira kuti muyike nsonga zatsopano pa adaputala kapena kunyamula ndikusuntha nsonga zomwe zagwiritsidwa ntchito mu chute ya zinyalala.

5.5 Machubu ndi machubu oyika

Dongosolo la Opentrons 4-in-1 Tube Rack limagwira ntchito ndi Opentrons Flex mwachisawawa. Kugwiritsa ntchito 4-in-1 chubu rack kumathandizira kuchepetsa ntchito yanu yokonzekera chifukwa zophatikizika zomwe zimapereka ndizokonzekera zokha kunja kwa bokosilo. Zambiri zimapezekanso mu Opentrons Labware Library.

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE
Tube ndi rack kuphatikiza
Opentrons 4-in-1 chubu rack imathandizira mitundu yosiyanasiyana yamachubu, payokha kapena mosiyanasiyana (voliyumu). Izi zikuphatikizapo: 6-chubu choyikapo 50 mL machubu (6 x 50 mL). 10-chubu kuphatikiza machubu anayi 50 mL machubu ndi asanu 15 mL machubu (4 x 50 mL, 6 x 15 mL). 15-chubu choyika cha 15 mL machubu (15 x 15 mL). 24-chubu rack kwa 0.5 mL, 1.5 mL, kapena 2 mL machubu (24 x 0.5 mL, 1.5 mL, 2 mL).
Zindikirani: Machubu onse ndi ozungulira okhala ndi V-woboola pakati (conical) pansi pokhapokha atawonetsedwa.

6-machubu rack
Mtundu wa chubu 6 Falcon 50 mL 6 NEST 50 mL
10-machubu rack
Mtundu wa chubu 4 Falcon 50 mL 6 Falcon 15 mL 4 NEST 50 mL 6 NEST 15 mL

API katundu dzina opentrons_6_tuberack_falcon_50ml_conical opentrons_6_tuberack_nest_50ml_conical
API load name opentrons_10_tuberack_falcon_4x50ml_6x15ml_conical opentrons_10_tuberack_nest_4x50ml_6x15ml_conical

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE

15-machubu rack
Mtundu wa chubu 15 Falcon 15 mL 15 NEST 15 mL

API katundu dzina opentrons_15_tuberack_falcon_15ml_conical opentrons_15_tuberack_nest_15ml_conical

24-machubu rack

Mtundu wa Tube
24 Eppendorf Safe-Lock 1.5 mL 24 Eppendorf Safe-Lock 2 mL, pansi ngati U-24 generic 2 mL screw cap 24 NEST 0.5 mL screw cap 24 NEST 1.5 mL screw cap 24 NEST 1.5 mL snap 24 m2 kapu NEST 24 mL snap cap, pansi ngati U

API katundu dzina opentrons_24_tuberack_eppendorf_1.5ml_safelock_snapcap opentrons_24_tuberack_eppendorf_2ml_safelock_snapcap
opentrons_24_tuberack_generic_2ml_screwcap opentrons_24_tuberack_nest_0.5ml_screwcap opentrons_24_tuberack_nest_1.5ml_screwcap opentrons_24_tuberack_nest_1.5ml_snapcap opentrons_24_tuberack_nest_2ml_screwcap opentrons_24_tuberack_nest_2ml_snapcap

Tanthauzo la Tube rack API
Laibulale ya Opentrons Labware imatanthauzira mawonekedwe a ma chubu olembedwa pamwambapa mu JSON yosiyana files. Loboti ya Flex ndi Opentrons Python API imadalira matanthauzo a JSON kuti agwire ntchito ndi labware yogwiritsidwa ntchito ndi ma protocol anu. Za example, pogwira ntchito ndi API, ntchito ya ProtocolContext.load_labware imavomereza mayina a labware awa ngati magawo ovomerezeka mu code yanu. Maina olemetsa a API olumikizidwa amalumikizana ndi matanthauzo a labware ya chubu munkhokwe ya Opentrons GitHub.

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE
Mwambo chubu pachiyika labware
Yesani kupanga matanthauzidwe a labware pogwiritsa ntchito Opentrons Labware Creator ngati chubu ndi rack zomwe mukufuna kugwiritsa ntchito sizinalembedwe apa. Kutanthauzira kwachizolowezi kumaphatikiza miyeso yonse, metadata, mawonekedwe, kuchuluka kwa volumetric, ndi zina zambiri mu JSON. file. Opentrons Flex imawerenga izi kuti mumvetsetse momwe mungagwirire ntchito ndi labure yanu. Onani gawo la Custom Labware Definitions kuti mudziwe zambiri.
5.6 zitsulo za aluminiyamu
Aluminium imatchinga sitimayo ndi Temperature Module GEN2 ndipo itha kugulidwa padera ngati magawo atatu. Choyikacho chimaphatikizapo mbale yapansi yapansi, chipika cha 24-chitsime, ndi chipika cha 96-chitsime.
Opentrons Flex imagwiritsa ntchito midadada ya aluminiyamu kuti igwire sample machubu ndi mbale zitsime pa Temperature Module kapena mwachindunji pa sitimayo. Mukagwiritsidwa ntchito ndi Temperature Module, midadada ya aluminiyamu imatha kusunga sample machubu, PCR n'kupanga, kapena mbale pa kutentha kosasintha pakati pa 4 °C ndi 95 °C.
Lathyathyathya pansi mbale
Chipinda chapansi chapansi cha zombo za Flex chokhala ndi Temperature Module's caddy ndipo chimagwirizana ndi mbale zosiyanasiyana za ANSI/SLAS. Mbale yathyathyathyayi imasiyana ndi mbale yomwe imanyamula ndi Temperature Module yokha kapena magawo atatu osiyana. Iwo zimaonetsa yotakata ntchito pamwamba ndi chamfered ngodya tatifupi. Izi zimathandizira kupititsa patsogolo magwiridwe antchito a Opentrons Flex Gripper posuntha labware pa mbale.
Mutha kudziwa kuti ndi mbale yathyathyathya iti yomwe muli nayo chifukwa ya Flex ili ndi mawu oti "Opentrons Flex" pamwamba pake. Mmodzi wa OT-2 alibe.

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE

24-chitsime cha aluminiyamu
Chitsime cha 24-chitsime chimagwiritsidwa ntchito ndi munthu aliyense sampndi mbale. Za example, amavomereza sampndi mbale kuti:
Khalani ndi V-zoboola pakati kapena U. Sungani zomwe zili mkati ndi snap cap kapena zotsekera zotsekera. Gwirani madzi mu mphamvu ya 0.5 mL, 1.5 mL, ndi 2 mL.

96-chitsime cha aluminiyamu
Chitsime cha 96-chitsime chimathandizira mitundu yosiyanasiyana ya mbale zachitsime. Za example, imavomereza mbale zabwino zomwe ndi:
Kuchokera kwa opanga bwino mbale ngati Bio-Rad ndi NEST.
Zopangidwa ndi zokhala ngati V, pansi ngati U, kapena pansi.
Zopangidwa ndi zitsime za 100 µL kapena 200 μL.
Zimagwirizananso ndi generic PCR strips.

Ma adapter okha
Thermal block Flex flat bottom plate 24-well aluminium block 96-well aluminium block

API katundu dzina opentrons_aluminium_flat_bottom_plate Onani kuphatikiza labware pansipa. opentrons_96_well_aluminium_block

Kuphatikizika kwa aluminium block labware
Laibulale ya Opentrons Labware imathandizira kuphatikizika kwa block, vial, ndi chitsime chotsatira, zomwe zimatanthauzidwanso mu tanthauzo la JSON labware. files. The Flex robot ndi Opentrons Python API

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE
dalirani matanthauzidwe a JSON awa kuti mugwire ntchito ndi labware yogwiritsidwa ntchito ndi ma protocol anu. Za example, pogwira ntchito ndi API, ntchito ya ProtocolContext.load_labware imavomereza mayina a labware awa ngati magawo ovomerezeka mu code yanu. Matebulo omwe ali m'munsimu amatchula zophatikizira za block/container ndi mayina okhudzana ndi API. Maulalo amalumikizana ndi matanthauzidwe ofanana a JSON munkhokwe ya Opentros GitHub.
Zindikirani: Machubu onse ali ndi pansi ngati V pokhapokha atawonetsedwa.

24-well aluminium block labware kuphatikiza

24-well block contents Generic 2 mL screw cap NEST 0.5 mL screw cap NEST 1.5 mL screw cap NEST 1.5 mL snap cap NEST 2 mL screw cap NEST 2 mL snap cap, pansi ngati U-

API katundu dzina opentrons_24_aluminumblock_generic_2ml_screwcap opentrons_24_aluminumblock_nest_0.5ml_screwcap opentrons_24_aluminumblock_nest_1.5ml_screwcap opentrons_24_aluminiumblock_nest_1.5mlcapnest_scrowp24 ns_2_aluminiumblock_nest_24ml_snapcap

96-well aluminium block labware kuphatikiza

96-chitsime chotchinga mkati Bio-Rad chitsime mbale 200 µL Generic PCR Mzere 200 µL NEST mbale mbale 100 µL

API katundu dzina opentrons_96_aluminumblock_biorad_wellplate_200uL opentrons_96_aluminumblock_generic_pcr_strip_200uL opentrons_96_aluminumblock_nest_wellplate_100uL

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE

5.7 Labware ndi Opentrons Flex Gripper
Ngakhale Opentrons Flex imagwira ntchito ndi zonse zomwe zili mu Labware Library, Opentrons Flex Gripper imagwirizana ndi zinthu za labware zokha. Pakadali pano, Gripper imakonzedwa kuti igwiritsidwe ntchito ndi zinthu zotsatirazi za labware.

Gulu la Labwara Mbale Zakuya Zakuya Zovala Mokwanira 96 Mbale
Tip Racks (nsonga zosasefedwa komanso zosefedwa)

Mitundu
NEST 96 Deep Well Plate 2 ml
Opentrons Tough 96 Well Plate 200 µL PCR Full Skirt NEST 96 Well Plate 200 µL Flat
Opentrons Flex 96 Tip Rack 50 µL Opentrons Flex 96 Tip Rack 200 µL Opentrons Flex 96 Tip Rack 1000 µL

Zindikirani: Kuti mupeze zotsatira zabwino, gwiritsani ntchito Flex Gripper kokha ndi labware yomwe yatchulidwa pamwambapa. Flex Gripper itha kugwira ntchito ndi ma labure ena ogwirizana ndi ANSI/SLAS, koma izi sizovomerezeka.

5.8 Tanthauzo la labware mwamakonda
Monga tafotokozera koyambirira kwa mutu uno, labware yachizolowezi ndi labware yomwe sinalembedwe mu Opentrons Labware Library. Mutha kugwiritsa ntchito zinthu zina wamba kapena zapadera za labware ndi Flex poyezera molondola ndi kujambula mawonekedwe a chinthucho ndikusunga zomwezo mu JSON. file. Mukatumizidwa mu pulogalamuyi, Flex ndi API imagwiritsa ntchito deta ya JSON kuti igwirizane ndi labware yanu. Opentrons imapereka zida ndi ntchito, zomwe tikambirana pansipa, kukuthandizani kugwiritsa ntchito Flex yokhala ndi labure.

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE

Kupanga matanthauzo a labware
Zida ndi ntchito za Opentrons zimakuthandizani kuti muzitha kuzifikira. Izi zimatengera luso losiyanasiyana komanso njira zogwirira ntchito. Kupanga labware yanu, ndikuigwiritsa ntchito ndi Opentrons Flex, kumathandiza kuti loboti ikhale yosunthika komanso yamphamvu yowonjezera labu yanu.

MLENGI WA CUSTOM LABWARE
The Custom Labware Mlengi alibe code, web-chida chokhazikitsidwa chomwe chimagwiritsa ntchito mawonekedwe owonetsera kuti akuthandizeni kupanga tanthauzo la labware file. Labware Mlengi amapanga JSON labware tanthauzo file zomwe mumalowetsa mu Opentrons App. Pambuyo pake, labware yanu yachizolowezi imapezeka ku Flex robot ndi Python API.

CUSTOM LABWARE SERVICE
Lumikizanani nafe ngati labware yomwe mukufuna kugwiritsa ntchito palibe mulaibulale, ngati simungathe kupanga matanthauzidwe anu, kapena chifukwa china chake chimakhala ndi mawonekedwe, kukula kwake, kapena zolakwika zina zomwe zafotokozedwa pansipa.

Labware mutha kutanthauzira mu Labware Creator
; Zitsime ndi machubu ndizofanana komanso zofanana. ; Mizere yonse imakhala yofanana
(danga lapakati pa mizere ndilofanana).
; Mizati yonse ndi yofanana (danga pakati pa mizati ndi lofanana).
; Imakwanira bwino pagawo limodzi.

Labware Opentrons iyenera kufotokozera; Zitsime ndi mawonekedwe a chubu amasiyana. ; Mizere siyikhala yotalikirana.
; Mizati siyili molingana.
; Zocheperako pang'ono kuposa sikelo imodzi (imafuna adaputala) kapena mipata yambiri.

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE
Nawa zithunzi zomwe zimakuthandizani kuti muwone m'maganizo mwanu wakaleampzomwe tafotokozazi. Nthawi Zonse Milingo yonse imakhala yofanana ndipo mizere yonse imakhala yofanana. Mizati sifunika kukhala ndi mipata yofanana ndi mizere.
Nthawi zonse Gululi siliyenera kukhala pakatikati pa labware.
Mizere Yosakhazikika imakhala ndi mipata yofanana koma mizati siyikhala yofanana.
Mizere/mizere yosagwirizana ndi mipata yofanana koma zitsime sizifanana.
Zosakhazikika Pali magulu opitilira umodzi.
Gulu lathu la labware lidzagwira ntchito kuti limvetsetse zosowa zanu ndikupangirani matanthauzidwe a labware. Onani zolemba zothandizira Kufunsira Tanthauzo La Labware Yachizolowezi ndi Fomu Yofunsira Mwambo Labware kuti mumve zambiri. Izi ndi ntchito zolipira.

Mtengo wa OPENTRONS FLEX

MUTU 5: LABWARE

PYTHON API
Ngakhale simungathe kupanga labware ndi API yathu, mutha kugwiritsa ntchito labware ndi njira zomwe zilipo za API. Komabe, muyenera kufotokozera labware yanu kaye ndikuyilowetsa mu Opentrons App. Mukangowonjezera labu yanu ku Opentrons App, ikupezeka ku Python API ndi loboti. Onani gawo la Custom Labware Definitions la zolemba za Python API kuti mumve zambiri. Kuti mudziwe zambiri za kulemba zolemba za protocol ndi API, onani gawo la Python Protocol API mu mutu wa Protocol Development.
JSON labware schema
A JSON file ndiye pulani ya Opentrons yokhazikika komanso labware yokhazikika. Izi file lili ndi kukonza deta ya labware molingana ndi mapangidwe omwe akhazikitsidwa ndi schema yosasinthika.
Chiwembu ndi chimango

Zolemba / Zothandizira

opentrons FLEX FLEX Opentrons Flex Open Source Liquid Handling Robot [pdf] Buku la Malangizo
FLEX Opentrons Flex Open Source Liquid Handling Robot, FLEX, Opentrons Flex Open Source Liquid Handling Robot, Flex Open Source Liquid Handling Robot, Open Source Liquid Handling Robot, Gwero la Liquid Handling Robot, Liquid Handling Robot, Liquid Handling Robot, Robot

Maumboni

Buku Logwiritsa Ntchito

FLEX Opentrons Flex Open Source Liquid Handling Robot

Opentrons Flex

Zofotokozera:

Malangizo Ogwiritsira Ntchito Zogulitsa:

1. Kuyika ndi kusanja kwa zida:

2. Kusamuka:

3. Zolumikizana:

4. Wopanga Protocol:

5. Python Protocol API:

6. OT-2 Protocols:

Mafunso Ofunsidwa Kawirikawiri (FAQ):

Q: Kodi ndimathetsa bwanji ngati loboti silikuyenda ngati
kuyembekezera?

Q: Kodi ndingagwiritse ntchito ma pipette ndi Opentrons Flex?

Zolemba / Zothandizira

Maumboni

Siyani ndemanga

Letsani yankho

FLEX Opentrons Flex Open Source Liquid Handling Robot

Opentrons Flex

Zofotokozera:

Malangizo Ogwiritsira Ntchito Zogulitsa:

1. Kuyika ndi kusanja kwa zida:

2. Kusamuka:

3. Zolumikizana:

4. Wopanga Protocol:

5. Python Protocol API:

6. OT-2 Protocols:

Mafunso Ofunsidwa Kawirikawiri (FAQ):

Q: Kodi ndimathetsa bwanji ngati loboti silikuyenda ngati kuyembekezera?

Q: Kodi ndingagwiritse ntchito ma pipette ndi Opentrons Flex?

Zolemba / Zothandizira

Maumboni

Zolemba Zogwirizana

Siyani ndemanga

Letsani yankho

Q: Kodi ndimathetsa bwanji ngati loboti silikuyenda ngati
kuyembekezera?