I-MacroArray ALLERGY XPLORER I-Macro Array Diagnostics
Imininingwane
- Igama lomkhiqizo: Isisekelo se-UDI-DI 91201229202JQ
- Izinombolo Eziyisethenjwa: REF 02-2001-01, 02-5001-01
- Ukusetshenziswa Okuhlosiwe: Ukutholwa kwe-allergen-specific IgE (sIgE) ngobuningi kanye nesamba se-IgE (tIgE) semi-quantitatively
- Abasebenzisi: Izisebenzi zaselabhorethri eziqeqeshiwe kanye nochwepheshe bezokwelapha elabhorethri yezokwelapha
- Isitoreji: Amakhithi ama-reagents azinzile izinyanga eziyi-6 ngemuva kokuvulwa
Imiyalo yokusetshenziswa komkhiqizo
Isimiso Senqubo
Umkhiqizo uthola i-IgE eqondene ne-allergen ngobuningi kanye nesamba se-IgE semi-quantitatively.
Ukuthunyelwa kanye Nesitoreji
Qinisekisa ukuthi ama-reagents ekhithi agcinwa njengoba kukhonjisiwe futhi asetshenziswa phakathi nezinyanga eziyisi-6 zokuvulwa.
Ukulahlwa kwemfucuza:
Landela izinqubo ezifanele zokulahla imfucuza ngokwemithethonqubo.
Izingxenye Zekhithi
Bheka kumanuwali yomsebenzisi ukuze uthole ulwazi oluningiliziwe ngezingxenye zekhithi.
Izisetshenziswa ezidingekayo
Ukuhlaziya okwenziwa ngesandla: Qinisekisa ukuthi unempahla edingekayo ehlinzekwa ngumkhiqizi.
Ukuhlaziya okuzenzakalelayo: Sebenzisa idivayisi engu-MAX, Isixazululo sokuwasha, i-Stop Solution, i-RAPTOR SERVER Analysis Software, kanye ne-PC/Laptop. Landela imiyalelo yesondlo ngokucophelela.
Ukuphatha ama-Arrays
Landela imiyalelo enikeziwe yokuphatha ama-array ngokucophelela ukuze uqinisekise imiphumela enembile.
Izexwayiso kanye nezinyathelo zokuqapha
- Gqoka izinto zokuzivikela ezifanele njengokuvikela isandla namehlo namajazi elebhu.
- Phatha ama-reagents kanye ne-sampNgokulandela izinqubo ezinhle zaselabhorethri.
- Phatha zonke izinto eziwumthombo womuntu njengeziyingozi futhi uziphathe ngokucophelela.
FAQ
- Q: Ama-reagents ekhithi azinzile isikhathi esingakanani?
A: Ama-reagents ekhithi azinzile izinyanga eziyi-6 ngemuva kokuvulwa lapho egcinwe ngaphansi kwezimo ezibonisiwe. - Umbuzo: Ubani ongasebenzisa lo mkhiqizo?
IMP: Lo mkhiqizo uhloselwe ukusetshenziswa izisebenzi zaselabhorethri eziqeqeshiwe kanye nochwepheshe bezokwelapha endaweni yaselabhorethri yezokwelapha.
www.madx.com
I-ALLERGY XPLORER (ALEX²) UMYALELO WOKUSEBENZISA
DESCRIPTION
I-Allergy Xplorer (ALEX²) i-Enzyme-Linked Immunosorbent Assay (ELISA) - izivivinyo zokuxilonga ezisekelwe ku-in-vitro zokulinganisa ubuningi be-allergen-specific IgE (sIgE).
Lo Myalelo Wokusetshenziswa usebenza kule mikhiqizo elandelayo:
| I-UDI-DI eyisisekelo | REF | Umkhiqizo |
| 91201229202JQ | 02-2001-01 | I-ALEX² yokuhlaziya okungu-20 |
| 02-5001-01 | I-ALEX² yokuhlaziya okungu-50 |
INHLOSO OKUHLOSIWEYO
I-ALEX² Allergy Xplorer iyikhithi yokuhlola esetshenziselwa ukuhlolwa kwe-in-vitro kwe-serum yomuntu noma i-plasma (ngaphandle kwe-EDTA-plasma) ukuze kuhlinzekwe ulwazi olusiza ukuxilonga iziguli eziphethwe izifo ezixhunywe yi-IgE ngokuhambisana nokunye okutholwe emitholampilo noma imiphumela yokuhlolwa kokuxilonga. .
Idivayisi yezokwelapha ye-IVD ithola i-allergen-specific IgE (sIgE) ngobuningi kanye nesamba se-IgE (tIgE) ngokwe-semi-quantitatively. Umkhiqizo usetshenziswa izisebenzi zaselabhorethri eziqeqeshiwe kanye nochwepheshe bezokwelapha elabhorethri yezokwelapha.
ISIFINYEZO NENCAZELO YESIVIVINYO
Ukungezwani nokungezwani komzimba uhlobo olusheshayo lwe-hypersensitivity yokusabela futhi kulamula amasosha omzimba esigaba se-IgE sama-immunoglobulins. Ngemva kokuchayeka ezintweni ezithile ezingezwani nomzimba, ukukhululwa kwe-histamine okuphakathi kwe-IgE nabanye abaxhumanisi kuma-mast cell kanye nama-basophils kubangela ukubonakaliswa kwemitholampilo njengesifuba somoya, i-allergic rhino-conjunctivitis, i-atopic eczema, nezimpawu zesisu [1]. Ngakho-ke, iphethini yokuzwela enemininingwane emithonjeni ethile isiza ekuhlolweni kweziguli eziguliswa yizinto ezithile [2-6]. Awukho umkhawulo kusibalo sabantu abahlolwayo. Lapho kwakhiwa izivivinyo ze-IgE, ubudala nobulili ngokuvamile akubhekwa njengezici ezibalulekile ngoba amazinga e-IgE, akalwa kulezi zivivinyo, awahlukani kakhulu ngokusekelwe kulezi zibalo zabantu.
Yonke imithombo ye-allergen yohlobo lwe-I imbozwe yi-ALEX². Uhlu oluphelele lwe-ALEX² yokungezwani komzimba nezinto ezingezwani nazo zingatholakala ngaphansi kwalo myalelo.
Ulwazi olubalulekile lomsebenzisi!
Ukuze usebenzise kahle i-ALEX², kuyadingeka ukuthi umsebenzisi afunde ngokucophelela futhi alandele le miyalo ukuze ayisebenzise. Umkhiqizi akanaso isikweletu nganoma yikuphi ukusetshenziswa kwalesi simiso sokuhlola esingachazwanga kule dokhumenti noma ukuguqulwa komsebenzisi wesistimu yokuhlola.
Qaphela: Ikhithi ehlukile engu-02-2001-01 yokuhlolwa kwe-ALEX² (20 Arrays) ihloselwe ngokukhethekile ukucutshungulwa mathupha. Ukuze usebenzise lokhu okuhlukile kwekhithi ye-ALEX² ene-MAX 9k ezenzakalelayo, Isixazululo Sokugeza (REF 00-5003-01) kanye ne-Stop Solution (REF 00-5007-01) zidinga uku-odwa ngokuhlukana. Lonke ulwazi olwengeziwe lomkhiqizo lungatholakala emiyalweni ehambisanayo yokusetshenziswa: https://www.madx.com/extras.
Okuhlukile kwekhithi ye-ALEX² 02-5001-01 (amalungu afanayo angu-50) kungasetshenziselwa ukucubungula okuzenzakalelayo nge-MAX 9k (REF 17-0000-01) kanye nedivayisi ye-MAX 45k (REF 16-0000-01).
ISIMISO SENKQUBO
I-ALEX² ukuhlolwa kwe-immunoassay okusekelwe ku-Enzyme-Linked Immunosorbent Assay (ELISA). Okukhishiwe kwe-Allergen noma okungezwani ne-molecular, okuhlanganiswe nama-nanoparticles, kufakwa ngendlela ehlelekile esigabeni esiqinile esakha ama-macroscopic array. Okokuqala, izinto ezingezwani nezinhlayiyana eziboshwe yizinhlayiyana zisabela nge-IgE ethile ekhona ku-s yesiguliample. Ngemuva kokufukamela, i-IgE engaqondile iyagezwa. Inqubo iyaqhubeka ngokwengeza i-enzyme ene-antibody yokuthola i-IgE ene-enzyme eyenza inkimbinkimbi ene-IgE eboshwe ngezinhlayiyana. Ngemva kwesinyathelo sesibili sokugeza, i-substrate yengezwa eguqulwa ibe imvula engancibilikiyo, enombala nge-enzyme eboshwe ngama-antibody. Ekugcineni, ukusabela kwe-enzyme-substrate kuyekwa ngokungeza i-reagent evimbayo. Inani lemvula lilingana nokuhlushwa kwe-IgE ethile ku-s yesiguliample. Inqubo yokuhlola ilebhu ilandelwa ukutholwa kwesithombe nokuhlaziya kusetshenziswa isistimu yezandla (ImageXplorer) noma isistimu ezenzakalelayo (MAX 45k noma MAX 9k). Imiphumela yokuhlolwa ihlaziywa nge-RAPTOR SERVER Analysis Software futhi ibikwe kumayunithi okuphendula e-IgE (kUA/l). Isamba semiphumela ye-IgE nayo ibikwe kumayunithi okuphendula e-IgE (kU/l). I-RAPTOR SERVER iyatholakala kunguqulo 1, ngenombolo yenguqulo enamadijithi amane aphelele sicela ubhekisele ku-RAPTOR SERVER imprint etholakala ku- www.raptor-server.com/imprint.
UKUTHUMELA KANYE NESIGCINI
Ukuthunyelwa kwe-ALEX² kwenzeka ezimweni zokushisa ezizungezile. Noma kunjalo, ikhithi kufanele igcinwe ngokushesha lapho ihanjiswa ku-2-8 ° C. Igcinwe kahle, i-ALEX² kanye nezingxenye zayo zingasetshenziswa kuze kube idethi yokuphelelwa yisikhathi ebonisiwe.
Amakhithi ama-reagents azinzile izinyanga eziyi-6 ngemuva kokuvulwa (ngezimo ezikhonjisiwe zokugcina).
UKULAHLWA KWEMFUCUZA
Lahla i-ALEX² cartridge esetshenzisiwe kanye nezingxenye zekhithi ezingasetshenzisiwe ezinodoti wamakhemikhali waseselabhorethri. Landela yonke imithetho kazwelonke, yesifunda, neyendawo mayelana nokuchithwa.
UHLU LWEZIMFANEKISO
IZINTO ZEKHITHI
Ingxenye ngayinye (i-reagent) izinzile kuze kube yidethi eshiwo kulebula yengxenye ngayinye. Akunconywa ukuhlanganisa noma yimaphi ama-reagents avela kukhithi ehlukene. Ukuze uthole uhlu lwezinto ezingezwani nomzimba kanye nokungezwani komzimba namamolekyuli okunganyakaziswanga ku-ALEX² array, sicela uxhumane support@madx.com.
| Izingxenye zekhithi REF 02-2001-01 | Okuqukethwe | Izakhiwo |
| I-ALEX² Cartridge | 2 Amabhamuza ku-10 ALEX² ekuhlaziyeni okungu-20 sekukonke.
Ukulinganisa ngejika eliyinhloko kutholakala nge-RAPTOR SERVER Isofthiwe yokuhlaziya. |
Ilungele ukusetshenziswa. Gcina ku-2-8°C kuze kube yidethi yokuphelelwa yisikhathi. |
| I-ALEX² Sample Diluent | Ibhodlela elingu-1 ku-9 ml | Ilungele ukusetshenziswa. Gcina ku-2-8°C kuze kube yidethi yokuphelelwa yisikhathi. Vumela i-reagent ukuthi ifinyelele izinga lokushisa legumbi ngaphambi kokusetshenziswa. I-reagent evuliwe izinzile izinyanga ezingu-6 ku-2-8 ° C, ihlanganisa i-CCD inhibitor. |
| Ukugeza Isixazululo | Ibhodlela elingu-2 ku-50 ml | Ilungele ukusetshenziswa. Gcina ku-2-8°C kuze kube yidethi yokuphelelwa yisikhathi. Vumela i-reagent ukuthi ifinyelele izinga lokushisa legumbi ngaphambi kokusetshenziswa. I-reagent evuliwe izinzile izinyanga eziyi-6 ku-2-8°C. |
| Izingxenye zekhithi REF 02-2001-01 | Okuqukethwe | Izakhiwo |
| I-ALEX² I-antibody yokutholwa | Ibhodlela elingu-1 ku-11 ml | Ilungele ukusetshenziswa. Gcina ku-2-8°C kuze kube yidethi yokuphelelwa yisikhathi. Vumela i-reagent ukuthi ifinyelele izinga lokushisa legumbi ngaphambi kokusetshenziswa. I-reagent evuliwe izinzile izinyanga eziyi-6 ku-2-8°C. |
| ALEX² Isixazululo se-Substrate | Ibhodlela elingu-1 ku-11 ml | Ilungele ukusetshenziswa. Gcina ku-2-8°C kuze kube yidethi yokuphelelwa yisikhathi. Vumela i-reagent ukuthi ifinyelele izinga lokushisa legumbi ngaphambi kokusetshenziswa. I-reagent evuliwe izinzile izinyanga eziyi-6 ku-2-8°C. |
| (ALEX²) Misa Isixazululo | Ibhodlela elingu-1 ku-2.4 ml | Ilungele ukusetshenziswa. Gcina ku-2-8°C kuze kube yidethi yokuphelelwa yisikhathi. Vumela i-reagent ukuthi ifinyelele izinga lokushisa legumbi ngaphambi kokusetshenziswa. I-reagent evuliwe izinzile izinyanga ezingu-6 ku-2-8°C. Ingase ibonakale njengesixazululo se-turbid ngemva kokugcinwa isikhathi eside. Lokhu akunawo umthelela emiphumeleni. |
| Izingxenye zekhithi REF 02-5001-01 | Okuqukethwe | Izakhiwo |
| I-ALEX² Cartridge | 5 Amabhamuza ku-10 ALEX² ekuhlaziyeni okungu-50 sekukonke.
Ukulinganisa ngejika eliyinhloko elitholakala nge-RAPTOR SERVER Analysis Software. |
Ilungele ukusetshenziswa. Gcina ku-2-8°C kuze kube yidethi yokuphelelwa yisikhathi. |
| I-ALEX² Sample Diluent | Ibhodlela elingu-1 ku-30 ml | Ilungele ukusetshenziswa. Gcina ku-2-8°C kuze kube yidethi yokuphelelwa yisikhathi. Vumela i-reagent ukuthi ifinyelele izinga lokushisa legumbi ngaphambi kokusetshenziswa. I-reagent evuliwe izinzile izinyanga ezingu-6 ku-2-8 ° C, ihlanganisa i-CCD inhibitor. |
| Ukugeza Isixazululo | 4 x iqo. Ibhodlela elingu-1 ukuya ku-250 ml | Gcina ku-2-8°C kuze kube yidethi yokuphelelwa yisikhathi. Nciphisa 1 kuya ku-4 ngamanzi ancibilikisiwe ngaphambi kokusetshenziswa. Vumela i-reagent ukuthi ifinyelele izinga lokushisa legumbi ngaphambi kokusetshenziswa. I-reagent evuliwe izinzile izinyanga ezingu-6 ku-2-8°C. |
| I-ALEX² I-antibody yokutholwa | Ibhodlela elingu-1 ku-30 ml | Ilungele ukusetshenziswa. Gcina ku-2-8°C kuze kube yidethi yokuphelelwa yisikhathi. Vumela i-reagent ukuthi ifinyelele izinga lokushisa legumbi ngaphambi kokusetshenziswa. I-reagent evuliwe izinzile izinyanga eziyi-6 ku-2-8°C. |
| Izingxenye zekhithi REF 02-5001-01 | Okuqukethwe | Izakhiwo |
| ALEX² Isixazululo se-Substrate | Ibhodlela elingu-1 ku-30 ml | Ilungele ukusetshenziswa. Gcina ku-2-8°C kuze kube yidethi yokuphelelwa yisikhathi. Vumela i-reagent ukuthi ifinyelele izinga lokushisa legumbi ngaphambi kokusetshenziswa. I-reagent evuliwe ngu
uzinze izinyanga eziyisi-6 ku-2-8°C. |
| (ALEX²) Misa Isixazululo | Ibhodlela elingu-1 ku-10 ml | Ilungele ukusetshenziswa. Gcina ku-2-8°C kuze kube yidethi yokuphelelwa yisikhathi. Vumela i-reagent ukuthi ifinyelele izinga lokushisa legumbi ngaphambi kokusetshenziswa. I-reagent evuliwe izinzile izinyanga ezingu-6 ku-2-8°C. Ingase ibonakale njengesixazululo se-turbid ngemva kokugcinwa isikhathi eside. Lokhu akunawo umthelela emiphumeleni. |
IZIHLOKO EZIDINGEKAYO ZOKUQUQUZWA NOKUHLAZIYA
Ukuhlaziywa Kwezandla
- I-ImageXplorer
- Isimeli (uyazikhethela)
- I-Lab Rocker (i-engeli yokuthambekela engu-8°, isivinini esidingekayo esingu-8 rpm)
- Igumbi lokufukamela (WxDxH – 35x25x2 cm)
- I-RAPTOR SERVER Analysis Software
- I-PC/Laptop
Izisetshenziswa ezidingekayo, ezinganikezwanga i-MADx:
- Amanzi Angenawo Amaminerali
- Amapayipi namathiphu (100 µl & 100 - 1000 µl)
Ukuhlaziya okuzenzakalelayo:
- Idivayisi engu-MAX (MAX 45k noma MAX 9k)
- Isixazululo sokugeza (REF 00-5003-01)
- Misa Isixazululo (REF 00-5007-01)
- I-RAPTOR SERVER Analysis Software
- I-PC/Laptop
Izinsizakalo zesondlo ngokwemiyalelo yomkhiqizi.
UKUPHATHA AMA-ARRAY
Ungathinti indawo yamalungu afanayo. Noma ikuphi ukonakala okubangelwa izinto ezibuthuntu noma ezicijile kungaphazamisa ukufunda okulungile kwemiphumela. Ungazitholi izithombe ze-ALEX² ngaphambi kokuthi uhlu lome ngokuphelele (lome ezingeni lokushisa legumbi).
IZIXWAYISO NEZIQINISEKISO
- Kunconywa ukuthi ugqoke isivikelo sezandla neso kanye namajazi elebhu futhi ulandele imikhuba emihle yaselabhorethri lapho ulungiselela futhi uphatha ama-reagents kanye s.ampLes.
- Ngokuhambisana nokusebenza okuhle kwelabhorethri, zonke izinto eziwumthombo womuntu kufanele zibhekwe njengezinamandla okuthelelanayo futhi zisingathwe ngezinyathelo zokuphepha ezifanayo nalezo zesiguli.ampLes.
- I-ALEX² SampI-le Diluent and Washing Solution iqukethe i-sodium azide (<0.1%) njengesilondolozi futhi kufanele iphathwe ngokucophelela. Ishidi ledatha yokuphepha liyatholakala uma ucelwa.
- I-(ALEX²) Stop Solution iqukethe i-Ethylenediaminetetraacetic acid (EDTA)-Solution futhi kufanele isingathwe ngokucophelela. Ishidi ledatha yokuphepha liyatholakala uma ucelwa.
- Okokusetshenziswa kwe-in-vitro diagnostic kuphela. Hhayi okokusetshenziswa kwangaphakathi noma kwangaphandle kubantu noma ezilwaneni.
- Izisebenzi eziqeqeshelwe umkhuba waselabhorethri kuphela okufanele zisebenzise le khithi.
- Lapho ufika, hlola izingxenye zekhithi ngomonakalo. Uma enye yezingxenye yonakalisiwe (isb. amabhodlela e-buffer), thintana ne-MADx (support@madx.com) noma umsabalalisi wangakini. Ungasebenzisi izingxenye zekhithi ezilimele, njengoba ukusetshenziswa kwazo kungaholela ekusebenzeni kabi kwekhithi.
- Ungasebenzisi ama-reagents ngemuva kwezinsuku zawo zokuphelelwa yisikhathi.
- Ungahlanganisi ama-reagents avela kumaqoqo ahlukene.
ELISA INQUBO
Ukulungiselela
Ukulungiswa kwe-sampI-Serum noma i-plasma (i-heparin, i-citrate, ayikho i-EDTA) sampIgazi le-capillary noma le-venous lingasetshenziswa. Igazi samples ingaqoqwa kusetshenziswa izinqubo ezijwayelekile. Isitolo sampngaphansi kwe-2-8°C kuze kube yisonto elilodwa. Gcina i-serum ne-plasma sampngaphansi kokungu-20°C ukuze igcinwe isikhathi eside. Ukuthunyelwa kwe-serum/plasma sampUkushisa okuphansi kuyasebenza. Vumela njalo u-sampukuze ufinyelele izinga lokushisa ekamelweni ngaphambi kokusetshenziswa.
Ukulungiswa Kwesixazululo Sokugeza (kuphela REF 02-5001-01 kanye ne-REF 00-5003-01 uma sisetshenziswa nedivayisi ye-MAX): Thela okuqukethwe kwebhodlela elingu-1 le-Washing Solution esitsheni sokuwasha sensimbi. Gcwalisa amanzi ancibilikisiwe kuze kufike uphawu olubomvu bese uxuba ngokucophelela isitsha izikhathi eziningana ngaphandle kokukhiqiza igwebu. I-reagent evuliwe izinzile izinyanga ezingu-6 ku-2-8°C.
Igumbi lokufukamela: Vala isivalo sazo zonke izinyathelo zokuhlola ukuvimbela ukwehla komswakama.
Amapharamitha of Inqubo:
- 100µl sample + 400 µl I-ALEX² Sample Diluent
- 500 µl ALEX² I-antibody yokutholwa
- 500 µl ALEX² Isixazululo se-Substrate
- 100 µl (ALEX²) Stop Solution
- 4500 µl Isixazululo Sokugeza
Isikhathi sokuhlola cishe si-3 h 30 min (ngaphandle kokoma kohlu olucutshunguliwe).
Akunconyelwe ukwenza ama-assay amaningi kunalokho okungafakwa ngepayipi emizuzwini engu-8. Wonke ama-incubation enziwa ekamelweni lokushisa, 20-26 ° C.
Wonke ama-reagents kufanele asetshenziswe ekamelweni lokushisa (20-26 ° C). Ukuhlolwa akufanele kwenziwe elangeni eliqondile.
Lungisa igumbi lokufukamela
Vula i-incubation chamber bese ubeka amathawula ephepha engxenyeni engezansi. Gcoba amathawula wephepha ngamanzi ancibilikisiwe kuze kube yilapho izingxenye ezomile zamathawula wephepha zingabonakali.
Sample incubation/CCD inhibition
Khipha inombolo edingekayo yama-cartridge we-ALEX² bese uwabeka kubaphathi bohlelo. Engeza u-400 μl we-ALEX² Sample Diluent kukhathriji ngayinye. Engeza isiguli esingu-100 μlample kuma-cartridges. Qinisekisa ukuthi ikhambi eliwumphumela lisakazwa ngokulinganayo. Beka ama-cartridge egumbini lokufukamela elilungisiwe bese ubeka igumbi lokufukamela nama-cartridges ku-rocker lab ukuze ama-cartridges azamazame ohlangothini olude lwe-cartridge. Qala i-serum incubation nge-8 rpm amahora ama-2. Vala i-incubation chamber ngaphambi kokuqala i-lab rocker. Ngemuva kwamahora ama-2, khipha i-samples esitsheni sokuqoqa. Sula ngokucophelela amaconsi ku-cartridge usebenzisa ithawula lephepha.
Gwema ukuthinta indawo engaphezulu ngethawula lephepha! Gwema noma yikuphi ukudlula noma ukungcoliswa kwe-sampkancane phakathi kwama-cartridge we-ALEX² angawodwana!
Ongakukhetha noma okuhle kwe-Hom s LF (umaka weCCD): ngephrothokholi evamile ye-CCD yokuvimbela amasosha omzimba (njengoba kuchazwe esigabeni 2: sample incubation/CCD inhibition) ukusebenza kahle kokuvimbela i-CCD ngama-85%. Uma izinga eliphezulu lokuvinjelwa liyadingeka, lungiselela i-1 ml sample tube, engeza 400 μl ALEX² Sample Diluent kanye ne-100 μl serum. Fukamela imizuzu engama-30 (unganyakazi) bese uqhubeka nenqubo yokuhlola evamile.
Qaphela: Isinyathelo esengeziwe sokuvimbela i-CCD siholela ezimweni eziningi esilinganisweni sokuvimbela amasosha omzimba e-CCD angaphezu kwama-95%.
1a. Ukugeza I
Faka isixazululo sokugeza esingu-500 μl kukhatriji ngayinye bese ufukamela ku-rocker lab (ngo-8 rpm) imizuzu emi-5. Khipha Isixazululo Sokugeza esitsheni esiqoqayo bese ucindezela ngamandla amakhatriji esitakini samathawula ephepha omile. Sula ngokucophelela amaconsi asele kuma-cartridges usebenzisa ithawula lephepha.
Phinda lesi sinyathelo izikhathi ezingu-2 ngaphezulu.
Engeza i-antibody yokuthola
Engeza u-500 µl we-ALEX² I-Detection Antibody ku-cartridge ngayinye.
Qiniseka ukuthi indawo ephelele yamalungu afanayo imbozwe isixazululo se-ALEX² Detection Antibody.
Beka ama-cartridges ekamelweni lokufukamela ku-rocker lab bese ufukamela ngo-8 rpm imizuzu engu-30. Khipha isixazululo se-Detection Antibody esitsheni esiqoqiwe bese ucindezela ngamandla amakhatriji esitaki samathawula ephepha omile. Sula ngokucophelela amaconsi asele kuma-cartridges usebenzisa ithawula lephepha.
2a. Ukugeza II
Faka isixazululo sokugeza esingu-500 μl kukhatriji ngayinye bese ufukamela ku-rocker lab ngo-8 rpm imizuzu emi-5. Khipha Isixazululo Sokugeza esitsheni esiqoqayo bese ucindezela ngamandla amakhatriji esitakini samathawula ephepha omile. Sula ngokucophelela amaconsi asele kuma-cartridges usebenzisa ithawula lephepha.
Phinda lesi sinyathelo izikhathi ezingu-4 ngaphezulu.
3+4. Engeza i-ALEX² Isixazululo Se-Substrate futhi umise ukusabela kwe-substrate
Engeza u-500 μl we-ALEX² Substrate Solution kukhatriji ngayinye. Qala isibali sikhathi ngokugcwalisa i-cartridge yokuqala bese uqhubeke nokugcwaliswa kwama-cartridge asele. Qinisekisa ukuthi indawo ephelele ye-array imbozwe yi-Substrate Solution futhi ufukamele ama-array imizuzu eyi-8 ngqo ngaphandle kokuzamazama (i-lab rocker ngo-0 rpm futhi isendaweni evundlile).
Ngemva kwemizuzu engu-8 ngqo, engeza u-100 μl we-(ALEX²) Stop Solution kuwo wonke amakhathriji, uqale ngekhatriji yokuqala ukuze uqinisekise ukuthi wonke amalungu afanayo afukanyelwa ngesikhathi esifanayo ne-ALEX² Substrate Solution. Khulisa ngokucophelela ukuze usabalalise ngokulinganayo Isixazululo Sokumisa (ALEX²) ku-cartridges yamalungu afanayo, ngemva kokuthi (ALEX²) Isixazululo Sokumisa sifakwe ngamapayipi kuwo wonke amalungu afanayo. Ngemva kwalokho khipha (ALEX²) Isixazululo Se-Substrate/Stop kusuka kuma-cartridges bese ucindezela ngamandla ama-cartridge esitakini samathawula ephepha omile. Sula ngokucophelela noma imaphi amaconsi asele kuma-cartridges usebenzisa ithawula lephepha.
I-Lab Rocker AKUMELE INGABE IYADIKIZISE ngesikhathi sokufakwa kwe-substrate!
4a. Ukugeza III
Faka isixazululo sokugeza esingu-500 μl kukhatriji ngayinye bese ufukamela ku-rocker lab ngo-8 rpm imizuzwana engu-30. Khipha Isixazululo Sokugeza esitsheni esiqoqayo bese ucindezela ngamandla amakhatriji esitakini samathawula ephepha omile. Sula ngokucophelela noma imaphi amaconsi asele kuma-cartridges usebenzisa ithawula lephepha.
Ukuhlaziywa kwesithombe
Ngemva kokuphothula inqubo yokuhlola, yomisa emoyeni ama-arrays ekamelweni lokushisa kuze kube yilapho omile ngokuphelele (kungathatha kufika kumaminithi angu-45).
Ukomisa okuphelele kubalulekile ukuze kube nokuzwela kokuhlolwa. Amalungu afanayo omiswe ngokuphelele kuphela anikeza isignali efanelekile isilinganiso somsindo.
Okokugcina, amalungu afanayo omisiwe askeniwe nge-ImageXplorer noma idivayisi ye-MAX futhi ahlaziywe ngesofthiwe ye-RAPTOR SERVER Analysis (bona imininingwane encwadini ye-software ye-RAPTOR SERVER). I-RAPTOR SERVER Analysis Software iqinisekiswa kuphela ngokuhlanganiswa nethuluzi le-ImageXplorer kanye namadivayisi we-MAX, ngakho-ke i-MADx ayithathi noma yimiphi isibopho ngemiphumela, etholwe nanoma iyiphi enye idivayisi yokuthwebula izithombe (njengezikena).
I-Assay Calibration
I-ALEX² ijika eliyinhloko lokulinganisa lasungulwa ngokuhlolwa kwereferensi ngokumelene namalungiselelo e-serum ane-IgE ethile ngokumelene nama-antigen ahlukene amboza ububanzi obuhlosiwe bokulinganisa. Inqwaba yemingcele yokulinganisa ihlinzekwa yi-RAPTOR SERVER Analysis Software. Imiphumela yokuhlolwa kwe-ALEX² sigE ichazwa njenge-kUA/l. Isamba semiphumela ye-IgE singu-semi-quantitative futhi ibalwa kusukela esilinganisweni se-anti-IgE esinezici zokulinganisa eziningi, ezihlinzekwa yi-RAPTOR SERVER Analysis Software futhi zikhethwa ngokuya ngamakhodi e-QR acacisiwe kwilotho ethile.
Imingcele yejika yenkatho ngayinye ilungiswa uhlelo lokuhlola ireferensi yangaphakathi, ngokumelene namalungiselelo e-serum ahlolwe ku-ImmunoCAP (Thermo Fisher Scientific) ye-IgE ethile ngokumelene nezifo eziningana. Ngakho-ke imiphumela ye-ALEX² ilandeleka ngokungaqondile uma kuqhathaniswa nokulungiselelwa kwereferensi ye-WHO 11/234 yengqikithi ye-IgE.
Ukwehluka okuhlelekile kumaleveli esignali phakathi kwenkatho kwenziwa ngokujwayelekile ngokulinganisa kwe-heterologous ngokumelene nejika lesithenjwa le-IgE. Isici sokulungisa sisetshenziselwa ukulungisa ngokuhlelekile ukuchezuka kokulinganisa okukhethekile.
Ibanga Lokulinganisa
I-IgE eqondile: 0.3-50 kUA/l ubuningi
Isamba se-IgE: 20-2500 kU/l i-semi-quantitative
IKHWALITHI YOKULAWULA
Ukugcinwa kwamarekhodi ohlolo ngalunye
Ngokomkhuba omuhle waselabhorethri kuyanconywa ukuthi urekhode izinombolo zenkatho yawo wonke ama-reagents asetshenzisiwe.
Lawula Izifanekiso
Ngokokwenza okuhle kwelabhorethri kunconywa ukuthi ukulawulwa kwekhwalithi sampama-les afakwe phakathi kwezikhawu ezichaziwe. Amanani ayisethenjwa e-sera ethile etholakalayo yokulawula anganikezwa yi-MADx uma eceliwe.
UKUHLAZIYWA KWEDATHA
Ukuze kuhlaziywe isithombe samalungu afanayo acutshunguliwe, i-ImageXplorer noma idivayisi ye-MAX izosetshenziswa. Izithombe ze-ALEX² zihlaziywa ngokuzenzakalela kusetshenziswa i-RAPTOR SERVER Analysis Software futhi umbiko uyakhiqizwa ofinyeza imiphumela yomsebenzisi.
IMIPHUMELA
I-ALEX² ukuhlolwa komthamo kwe-ELISA kwe-IgE ethile kanye nendlela elinganiselwe ye-IgE ephelele. Amasosha omzimba e-Allergen-specific IgE avezwa njengamayunithi okuphendula e-IgE (kUA/l), isamba semiphumela ye-IgE njenge-kU/l. I-RAPTOR SERVER Analysis Software ibala ngokuzenzakalelayo futhi ibike imiphumela ye-sIgE (ngobuningi) kanye nemiphumela ye-tIgE (i-semi-quantitatively).
IMIKHAWULO YENKQUBO
Ukuxilongwa okuqinisekile komtholampilo kufanele kwenziwe kuphela ngokuhambisana nakho konke okutholakala emtholampilo ochwepheshe bezokwelapha futhi ngeke kusekelwe emiphumeleni yendlela eyodwa yokuxilonga kuphela.
Ezindaweni ezithile zokusetshenziswa (isib. ukungezwani nokudla), amasosha omzimba ajikelezayo e-IgE angase aqhubeke engabonakali nakuba ukubonakaliswa okungokomtholampilo kokungezwani nokudla okuthile okuthile okungezwani komzimba kungase kube khona, ngenxa yokuthi lezi zivikeli mzimba zingase ziqonde ezinto ezingezwani nezifo ezishintshwayo ngesikhathi sokucutshungulwa kwezimboni, ukupheka noma ukugayeka kokudla futhi ngenxa yalokho azikho ekudleni kwasekuqaleni isiguli esihlolelwa kona.
Imiphumela engemihle yobuthi ikhombisa kuphela amazinga angatholakali amasosha omzimba athile e-IgE (isb. ngenxa yokungavezwa isikhathi eside) futhi ayivimbeli ukuba khona kokuzwela okukhulu emitholampilo ekulumeni kwezinambuzane.
Ezinganeni, ikakhulukazi ezifika eminyakeni emi-2 ubudala, uhla olujwayelekile lwe-tigE luphansi kunentsha nakubantu abadala [7]. Ngakho-ke, kulindelekile ukuthi engxenyeni ephezulu yezingane ezingaphansi kweminyaka engu-2 ingqikithi yeleveli ye-IgE ingaphansi komkhawulo wokutholwa oshiwo. Lo mkhawulo awusebenzi esilinganisweni esithile se-IgE.
IZIMALI EZILINDELEKILE
Ukuhlobana okuseduze phakathi kwamazinga e-antibody e-IgE aqondene ne-allergen kanye nesifo sokungezwani komzimba kwaziwa kahle futhi kuchazwa kahle ezincwadini [1]. Isiguli ngasinye esizwakele sizokhombisa i-IgE profile uma ihlolwe nge-ALEX². Impendulo ye-IgE ngo-sampOkuncane okuvela kubantu abanempilo abangaguquki kuzoba ngaphansi kuka-0.3 kUA/l kokungezwani komzimba nemolekyuli eyodwa kanye nasezindaweni ezingezwani komzimba lapho kuhlolwa nge-ALEX². Indawo eyinkomba ye-IgE isiyonke kubantu abadala ingaphansi kwe-100 kU/l. Ukuzijwayeza okuhle kwelabhorethri kuncoma ukuthi ilabhorethri ngayinye isungule uhla lwayo lwamanani alindelekile.
IZIMPAWU ZOKUSEBENZA
Izici zokusebenza kanye Nesifinyezo Sokuphepha Nokusebenza kungatholakala ku-MADx webindawo: https://www.madx.com/extras.
IWARANTI
Idatha yokusebenza itholwe kusetshenziswa inqubo echazwe kule Miyalelo Yokusetshenziswa. Noma iluphi ushintsho noma ukuguqulwa enqubweni kungase kuthinte imiphumela futhi i-MacroArray Diagnostics iyazilahla zonke iziqinisekiso eziveziwe (okuhlanganisa newaranti eshiwo yokuthengiseka kanye nokufaneleka ukuze kusetshenziswe) kumcimbi onjalo. Ngenxa yalokho, i-MacroArray Diagnostics kanye nabasabalalisi bayo basendaweni ngeke babophezeleke ngomonakalo ongaqondile noma owumphumela kumcimbi onjalo.
IZIfinyezo
| U-ALEX | I-Allergy Xplorer |
| I-CCD | Izithako ezisebenzayo ze-carbohydrate |
| I-EDTA | I-Ethylenediaminetetraacetic acid |
| ELISA | I-Enzyme-Linked Immunosorbent Assay |
| IgE | Immunoglobulin E |
| IVD | Ukuxilongwa kwe-in-vitro |
| kU/l | Amayunithi ekhilo ilitha |
| kUA/l | Ikhilo lamayunithi e-IgE eqondene ne-allergen ilitha |
| I-MADx | I-MacroArray Diagnostics |
| REF | Inombolo yesithenjwa |
| rpm | Ifinyeza ngomzuzu |
| sIgE | I-Allergen-specific IgE |
| tIgE | Ingqikithi ye-IgE |
| µl | I-Microliter |
UHLU LWE-ALLERGEN ALEX²
Imikhiqizo ye-Allergen: Aca m, Aca s, Ach d, Ail a, All c, All s, Ama r, Amb a, Ana o, Api m, Art v, Ave s, Ber e, Bos d meat, Bos d milk, Bro p, Cam d, Can f ♂ umchamo, Can s, Cap a, Capq h epithelia, Car p, Che Car i, Car p, Che spp., Cic a, Cit s, Cla h, Clu h, Cor a pollen, Cuc p, Cup s, Cyn d, Dau c, Dol spp., Equ c milk, Equ c meat, Fag e, Fic b, Fic c, Fra e, Gad m, Gal d meat, Gald white, Gal d Hor, Jun g, Jun Len c, Lit s, Loc m, Lol spp., Lup a, Mac i, Man i, Mel g, Mor r, Mus a, Myt e, Ori v, Ory meat, Ory s, Ost e, Ovi a epithelia, Ovi a meat, Ovi a milk, Pan b, Pan m, Pap s, Paspp n, Perj. a, Pet c, Pha v, Phr c, Pim a, Pis s, Pla l, Pol d, Pop n, Pru av, Pru du, Pyr c, Raj c, Rat n, Rud spp., Sac c, Sal k, Sal s, Sco s, Sec c powder, Sec c pollen, Ses i, Sipps Sold, Sold epithel, Sus d meat, Ten m, Thu a, Trifo, Tri s, Tyr p, Ulm c, Urt d, Vac m, Ves v, Zea m flour
Izingxenye zemvelo ezihlanziwe: nAct d 1, nApi m 1, nAra h 1, nAra 3, nBos d 4, nBos d 5, nBos d 6, nBos d 8, nCan f 3, nCor a 9, nCor a 11, nCup a 1, nCry j 1, nEqu c 3, nFag e 2, nGad m 1, nGad m 2 + 3, nGal d 2, nGal 3, nGal d 4, nGal d 5, nGly m 5, nGly m 6, nJug r 4, nMac i 2S Albumin, nOle e 7 (RUO), nPap s 2S Albumin, nPis v 3, nPla a 2, nTri a aA_TI
Izingxenye ezihlanganisiwe: rAct d 10, rAct d 2, rAct d 5, rAln g 1, rAln g 4, rAlt a 1, rAlt a 6, rAmb a 1, rAmb a 4, rAna o 2, rAna o 3, rAni 1, 3 Ani, 1 Ani rApi g 2, rApi g 6, rApi m 10, rAra h 2, rAra h 6, rAra h 8, rAra h 9, rAra h 15, rArg r 1, rArt v 1, rArt fr 3, spr 1, spr 3, spr. 4. 6, rCan f 1, rCan f 1, rCan f 2, rCan f 6, rCan f Fel d 1 like, rCan s 2, rCav p 4, rChe a 5, rCla h 9, rClu h 10, rCor a 21 a5 r 2, rCor a 1 a2 r. a 4 (RUO), rCor a 6, rCra c 1, , rCuc m 3, rCyn d 1, rCyp c 1, rDau c 8, rDer f 1, rDer f 1.0103, rDer p 1.0401, rDer p 8, pr 12D 14, rDer p 6, rDer p 2, rDer p 1, rDer p 1, rEqu c 1, rEqu c 1, rFag s 2, rFel d 1, rFel d 10, rFel d 11, rFel 2 + 20Fra d rGal 21, rGly d 23, rGly m 5, rGly m 7, rHev b 1, rHev b 4, rHev b 1, rHev b 1, rHev b 2, rHev b 4, rHom s LF, Jurg r, Jur 7, Jur r 1, rJug 3, rLep d 1, rLol p 1, rMal d 2, rMal d 4, rMala s 8, rMala s 1, rMala s 3, rMal d 5, rMer a 6.02, 8 MrOs 11, i-rOle e 1, i-rOry c 2, i-rOry c 3, i-rOry c 6, i-rPar j 2, i-rPen m 1, i-rPen m 1, i-rPen m 3, i-rPen m 11, i-rPer a 5, i-rPhl p 6, i-rPhl p 2, i-phl 1, i-phl 1, rPhl p 1, rPhl p 1, rPhod 9, rPhod s 1, rPis v 2, rPis v 3, rPis v 2 (RUO), rPla a 1, rPla a 2, rPla l 3, Pr 4 pr , 7 Pru (RUO), rRaj c Parvalbumin, rSal k 1, rSal s 12, rSco s 2, rSes i 5.0101, rSin a 6, rSola l 7, rSus d 2, rThu a 1, rTri a 1, rTri a v Tyr 2, 4 1, rVit v 3, rXip g 1, rZea m 5
IZIKHOMBISI
- Hamilton, RG. (2008). Ukuhlolwa kwezifo zokungezwani komzimba womuntu. I-Clinical Immunology. 1471-1484. 10.1016/B978-0-323-04404-2.10100-9.
- I-Harwanegg C, i-Laffer S, i-Hiller R, i-Mueller MW, i-Kraft D, i-Spitzauer S, i-Valenta R. I-Microarrayed recombinant allergened yokuxilongwa kokungezwani komzimba. I-Clin Exp Allergy. 2003 Jan;33(1):7-13. doi: 10.1046/j.1365-2222.2003.01550.x. PMID: 12534543.
- Hiller R, Laffer S, Harwanegg C, Huber M, Schmidt WM, Twardosz A, Barletta B, Becker WM, Blaser K, Breiteneder H, Chapman M, Crameri R, Duchêne M, Ferreira F, Fiebig H, Hoffmann-Sommergruber K, King TP, Kleber-Janke T, Kurup VP, Lehrer SB, Lidholm J, Müller U, Pini C, Reese G, Scheiner O, Scheynius A, Shen HD, Spitzauer S, Suck R, Swoboda I, Thomas W, Tinghino R, Van Hage-Hamsten M, Virtanen T, Kraft D, Müller MW, Valenta R . Ama-aleji ama-Microarrayed: onogada bamasango okwelapha ukungezwani komzimba nezinto ezithile. I-FASEB J. 2002 Mar;16(3):414-6. doi: 10.1096/fj.01-0711fje. Epub 2002 Jan 14. PMID: 11790727
- Ferrer M, Sanz ML, Sastre J, Bartra J, del Cuvillo A, Montoro J, Jáuregui I, Dávila I, Mullol J, Valero A. Ukuxilongwa kwamangqamuzana ku-allergology: ukusetshenziswa kwenqubo ye-microarray. J Investig Allergol Clin Immunol. 2009;19 Izengezo 1:19-24. I-PMID: 19476050.
- Ott H, Fölster-Holst R, Merk HF, Baron JM. I-Allergen microarrays: ithuluzi elinoveli le-high-resolution IgE profiling kubantu abadala abane-atopic dermatitis. Eur J Dermatol. 2010 Jan-Feb;20(1):54-
61. doi: 10.1684/ejd.2010.0810. I-Epub 2009 Oct 2. PMID: 19801343. - I-Sastre J. Ukuxilongwa kwe-Molecular ku-allergies. I-Clin Exp Allergy. 2010 Okthoba;40(10):1442-60. doi: 10.1111/j.1365-2222.2010.03585.x. Epub 2010 Aug 2. PMID: 20682003.
- Martins TB, Bandhauer ME, Bunker AM, Roberts WL, Hill HR. Izikhawu ezintsha zereferensi yobuntwana nezabantu abadala ze-IgE ephelele. J Allergy Clin Immunol. 2014 Feb;133(2):589-91.
Ukuze uthole imininingwane ngezifundo zokuhlaziya nezemitholampilo ezenziwe bheka izici zokusebenza kokuthi https://www.madx.com/extras.
SHINTSHA UMLANDO
| Inguqulo | Incazelo | Ifaka esikhundleni |
| 11 | I-nGal d1 ishintshele ku-rGal d1; URL kubuyekezwe ku madx.com; I-CE ingezwe ngenombolo Yomkhandlu Owazisiwe; shintsha umlando wengeziwe | 10 |
© Copyright by MacroArray Diagnostics
I-MacroArray Diagnostics (MADx)
I-Lemböckgasse 59, Top 4
1230 Vienna, Austria
+43 (0)1 865 2573
www.madx.com
Inombolo yenguqulo: 02-IFU-01-EN-11 Ikhishwe: 09-2024
Isiqondisi Esisheshayo
I-MacroArray Diagnostics
I-Lemböckgasse 59, Top 4
1230 Vienna
madx.com
I-CRN 448974 g
Amadokhumenti / Izinsiza
 |
I-MacroArray ALLERGY XPLORER I-Macro Array Diagnostics [pdf] Iziyalezo 91201229202JQ, 02-2001-01, 02-5001-01, ALLERGY XPLORER Macro Array Diagnostics, ALLERGY XPLORER, Macro Array Diagnostics, Array Diagnostics, Diagnostics |
