MacroArray ALLERGY XPLORER Macro Array Diagnostics
Zvinotsanangurwa
- Zita reChigadzirwa: Basic UDI-DI 91201229202JQ
- Nhamba Dzekushandisa: REF 02-2001-01, 02-5001-01
- Kushandiswa Kwakanangana: Kuonekwa kwe-allergen-specific IgE (sIgE) yakawanda uye yakazara IgE (tIgE) semi-quantitatively
- Vashandisi: Vashandi vemurabhoritari vakadzidziswa uye nyanzvi dzekurapa murabhoritari yezvokurapa
- Kuchengetedza: Kit reagents yakagadzikana kwemwedzi mitanhatu mushure mekuvhurwa
Mirayiridzo Yekushandiswa Kwechigadzirwa
Nheyo yeMaitiro
Chigadzirwa chinoona allergen-chaiyo IgE muhuwandu uye yakazara IgE semi-huwandu.
Kutumira uye Kuchengeta
Ita shuwa kuti kit reagents inochengetwa sezvakaratidzwa uye inoshandiswa mukati memwedzi mitanhatu yekuvhurwa.
Kurasa tsvina:
Tevedzera nzira dzakakodzera dzekurasa tsvina sezviri pamirau.
Kit Zvikamu
Tarisa kubhuku rekushandisa kuti uwane ruzivo rwakadzama pamusoro pezvikamu zvekiti.
Chinodiwa Equipment
Manual Analysis: Ita shuwa kuti une michina inodiwa inopihwa nemugadziri.
Automatic Analysis: Shandisa MAX mudziyo, Washing Solution, Stop Solution, RAPTOR SERVER Analysis Software, uye PC/Laptop. Tevera mirairo yekuchengetedza zvakanyatsonaka.
Kubata kweArrays
Tevedza mirairo yakapihwa yekubata mitsara nemazvo kuti uve nechokwadi chemhedzisiro.
Yambiro uye Chengetedzo
- Pfeka giya rekudzivirira rakakodzera seruoko uye maziso ekudzivirira uye majasi erabhoritari.
- Bata reagents uye sampzvishoma kutevera maitiro akanaka e laboratori.
- Bata zvinhu zvese zviri kubva kuvanhu sezvingangotapurira utachiona uye zvibate nehungwaru.
FAQ
- Q: Inguva yakareba sei kit reagents yakagadzikana?
A: Kit reagents yakagadzikana kwemwedzi mitanhatu mushure mekuvhurwa kana ichichengetwa pasi pemamiriro akaratidzwa. - Mubvunzo: Ndiani anogona kushandisa chigadzirwa ichi?
A: Ichi chigadzirwa chakagadzirirwa kushandiswa nevashandi vakadzidziswa murabhoritari uye nyanzvi dzekurapa munzvimbo yemurabhoritari yezvokurapa.
www.madx.com
ALLERGY XPLORER (ALEX²) MIRAYIRIRO YEKUSHANDISA
DESCRIPTION
Iyo Allergy Xplorer (ALEX²) ndeye Enzyme-Yakabatanidzwa Immunosorbent Assay (ELISA) - yakavakirwa in-vitro diagnostic bvunzo dzehuwandu kuyerwa kweallegen-specific IgE (sIgE).
Uyu Murairo Wekushandisa unoshanda kune zvinotevera zvigadzirwa:
| Basic UDI-DI | REF | Product |
| 91201229202JQ | 02-2001-01 | ALEX² ye20 Ongororo |
| 02-5001-01 | ALEX² ye50 Ongororo |
CHINANGWA CHECHINANGWA
Iyo ALEX² Allergy Xplorer itest kit inoshandiswa kuongorora in-vitro yeserum yemunhu kana plasma (kunze kweEDTA-plasma) kupa ruzivo rwekubatsira kuongororwa kwevarwere vanorwara nezvirwere zveIgE-mediated pamwe nezvimwe zvakawanikwa mukiriniki kana mhedzisiro yekuongorora. .
Iyo IVD mudziyo wekurapa unoona allergen-chaiwo IgE (sIgE) yakawanda uye yakazara IgE (tIgE) semi-huwandu. Chigadzirwa chacho chinoshandiswa nevashandi vakadzidziswa marabhoritari uye nyanzvi dzekurapa murabhoritari yekurapa.
PFUPISO UYE TSANANGURO YEMUYEdzo
Allergic reactions imhando yekukurumidza I hypersensitivity reactions uye inopindirana nemasoja ekudzivirira chirwere ari ekirasi yeIgE yema immunoglobulins. Mushure mekuratidzwa kune chaiwo allergenic, IgE-mediated kuburitswa kwe histamine uye vamwe vayananisi kubva mast maseru uye basophils inoguma nekuratidzwa kwekiriniki kwakadai seasthma, allergic rhino-conjunctivitis, atopic eczema, uye zviratidzo zvemudumbu [1]. Naizvozvo, yakadzama sensitization maitiro kune chaiwo allergenic anobatsira mukuongororwa kweallergic varwere [2-6]. Hapana chinorambidzwa pahuwandu hwekuedzwa. Paunenge uchigadzira maIgE assays, zera uye bonde hazviwanzo kutariswa sezvinhu zvakakosha nekuti IgE mazinga, ayo anoyerwa mune aya assays, haana kunyanya kusiyana zvichienderana nehuwandu hwevanhu.
Ese makuru emhando I allergen masosi akafukidzwa neALEX². Rondedzero izere yeALEX² allergen maextracts uye molecular allergens inogona kuwanikwa pazasi pekuraira uku.
Ruzivo rwakakosha kumushandisi!
Kuti ishandise chaiyo ALEX², zvinodikanwa kuti mushandisi anyatso kuverenga uye kutevedzera iyi mirairo yekushandisa. Mugadziri haatore mhosva yekushandiswa kweiyi bvunzo system iyo isina kutsanangurwa mugwaro iri kana kugadziridzwa nemushandisi weye test system.
Chenjerera: Iyo kit musiyano 02-2001-01 yeALEX² bvunzo (20 Arrays) inongoitirwa manyorero ekugadzirisa. Kuti ushandise iyi ALEX² kit musiyano ine otomatiki MAX 9k, iyo Washing Solution (REF 00-5003-01) uye Stop Solution (REF 00-5007-01) inoda kuodha zvakasiyana. Yese rumwe ruzivo rwechigadzirwa runogona kuwanikwa mune inoenderana mirairo yekushandisa: https://www.madx.com/extras.
Iyo ALEX² kit musiyano 02-5001-01 (50 arrays) inogona kushandiswa kugadzira otomatiki neMAX 9k (REF 17-0000-01) pamwe neMAX 45k (REF 16-0000-01) mudziyo.
ZVINHU ZVINOITA KUTI
ALEX² ndeye immunoassay bvunzo yakavakirwa paEnzyme-Linked Immunosorbent Assay (ELISA). Allergen extracts kana molecular allergens, ayo akabatanidzwa kune nanoparticles, anoiswa mune yakarongeka fashoni pachikamu chakasimba ichigadzira macroscopic array. Chekutanga, ma particle-bound allergens anoita neIgE chaiyo iripo mumurwereample. Mushure mekuvharirwa, isina-chaiyo IgE inogezwa. Maitiro acho anoenderera mberi nekuwedzera enzyme-yakanyorwa anti-human IgE yekuona antibody iyo inoumba yakaoma ine particle-yakasungwa chaiyo IgE. Mushure mechikamu chechipiri chekugeza, substrate inowedzerwa iyo inoshandurwa kuita isinganyungudika, ine mavara emvura neiyo antibody-yakasungwa enzyme. Pakupedzisira, iyo enzyme-substrate reaction inomiswa nekuwedzera inovharira reagent. Huwandu hwekunaya kwemvura hunoenderana nehuwandu hweIgE chaiyo mumurwereample. Iyo lab yekuyedza maitiro inoteverwa nekutora mifananidzo uye ongororo uchishandisa ingave yemanyorero sisitimu (ImageXplorer) kana iyo otomatiki system (MAX 45k kana MAX 9k). Mhedzisiro yebvunzo inoongororwa neRAPTOR SERVER Analysis Software uye yakashumwa muIgE mhinduro zvikamu (kUA/l). Yese mibairo yeIgE inoshumwawo muIgE mhinduro zvikamu (kU/l). RAPTOR SERVER inowanikwa muvhezheni 1, kune izere nhamba ine manhamba eshanduro ndapota tarisa kune RAPTOR SERVER imprint iripo pa. www.raptor-server.com/imprint.
SHIPMENT UYE KUCHENGETA
Kutumirwa kweALEX² kunoitika pane ambient tembiricha mamiriro. Nekudaro, iyo kit inofanirwa kuchengetwa nekukasira pakuburitswa pa2-8 ° C. Yakachengetwa nemazvo, ALEX² uye zvikamu zvayo zvinogona kushandiswa kusvika zuva rakatarwa rekupera.
Kit reagents yakagadzikana kwemwedzi gumi nemiviri mushure mekuvhurwa (panzvimbo dzakaratidzwa dzekuchengetedza).
KURASA marara
Rasa yakashandiswa ALEX² cartridge uye zvinhu zvekiti zvisina kushandiswa nemarara emakemikari emurabhoritari. Tevera mitemo yese yenyika, yenyika, uye yemunharaunda maererano nekuraswa.
BHUKU REZVIRATIDZO
KIT COMPONENTS
Chimwe nechimwe chikamu (reagent) chakagadzikana kusvika zuva rataurwa pane chimwe nechimwe chechikamu chezita. Izvo hazvikurudzirwe kusanganisa chero reagents kubva akasiyana kit majeti. Kuti uwane rondedzero yeallergen extracts uye molecular allergens isingafambiswe pane ALEX² array, ndapota bata support@madx.com.
| Kit Zvikamu REF 02-2001-01 | Content | Properties |
| ALEX² Cartridge | 2 Blisters à 10 ALEX² ye20 ongororo yakazara.
Calibration kuburikidza ne master curve inowanikwa kuburikidza neRAPTOR SERVER Analysis Software. |
Yakagadzirira kushandiswa. Chengetedza pa2-8°C kusvika zuva rekupera. |
| ALEX² Sample Diluent | 1 bhodhoro kusvika 9 ml | Yakagadzirira kushandiswa. Chengetedza pa2-8°C kusvika zuva rekupera. Bvumira reagent kuti isvike mukamuri tembiricha isati yashandiswa. Yakavhurwa reagent yakagadzikana kwemwedzi mitanhatu pa 6-2 ° C, inosanganisira CCD inhibitor. |
| Washing Solution | 2 bhodhoro kusvika 50 ml | Yakagadzirira kushandiswa. Chengetedza pa2-8°C kusvika zuva rekupera. Bvumira reagent kuti isvike mukamuri tembiricha isati yashandiswa. Yakavhurwa reagent yakagadzikana kwemwedzi gumi nemiviri pa6-2°C. |
| Kit Zvikamu REF 02-2001-01 | Content | Properties |
| ALEX² Detection Antibody | 1 bhodhoro kusvika 11 ml | Yakagadzirira kushandiswa. Chengetedza pa2-8°C kusvika zuva rekupera. Bvumira reagent kuti isvike mukamuri tembiricha isati yashandiswa. Yakavhurwa reagent yakagadzikana kwemwedzi gumi nemiviri pa6-2°C. |
| ALEX² Substrate Solution | 1 bhodhoro kusvika 11 ml | Yakagadzirira kushandiswa. Chengetedza pa2-8°C kusvika zuva rekupera. Bvumira reagent kuti isvike mukamuri tembiricha isati yashandiswa. Yakavhurwa reagent yakagadzikana kwemwedzi gumi nemiviri pa6-2°C. |
| (ALEX²) Stop Solution | 1 bhodhoro kusvika 2.4 ml | Yakagadzirira kushandiswa. Chengetedza pa2-8°C kusvika zuva rekupera. Bvumira reagent kuti isvike mukamuri tembiricha isati yashandiswa. Yakavhurwa reagent yakagadzikana kwemwedzi mitanhatu pa6-2°C. Inogona kutaridzika senge turbid solution mushure mekuchengetedza kwenguva refu. Izvi hazvina zvazvinoita. |
| Kit Zvikamu REF 02-5001-01 | Content | Properties |
| ALEX² Cartridge | 5 Blisters à 10 ALEX² ye50 ongororo yakazara.
Calibration kuburikidza ne master curve inowanikwa kuburikidza neRAPTOR SERVER Analysis Software. |
Yakagadzirira kushandiswa. Chengetedza pa2-8°C kusvika zuva rekupera. |
| ALEX² Sample Diluent | 1 bhodhoro kusvika 30 ml | Yakagadzirira kushandiswa. Chengetedza pa2-8°C kusvika zuva rekupera. Bvumira reagent kuti isvike mukamuri tembiricha isati yashandiswa. Yakavhurwa reagent yakagadzikana kwemwedzi mitanhatu pa 6-2 ° C, inosanganisira CCD inhibitor. |
| Washing Solution | 4 x conc. 1 bhodhoro kusvika 250 ml | Chengetedza pa2-8°C kusvika zuva rekupera. Dilute 1 kusvika 4 nemvura ine demineralized usati washandisa. Bvumira reagent kuti isvike mukamuri tembiricha isati yashandiswa. Yakavhurwa reagent yakagadzikana kwemwedzi mitanhatu pa6-2°C. |
| ALEX² Detection Antibody | 1 bhodhoro kusvika 30 ml | Yakagadzirira kushandiswa. Chengetedza pa2-8°C kusvika zuva rekupera. Bvumira reagent kuti isvike mukamuri tembiricha isati yashandiswa. Yakavhurwa reagent yakagadzikana kwemwedzi gumi nemiviri pa6-2°C. |
| Kit Zvikamu REF 02-5001-01 | Content | Properties |
| ALEX² Substrate Solution | 1 bhodhoro kusvika 30 ml | Yakagadzirira kushandiswa. Chengetedza pa2-8°C kusvika zuva rekupera. Bvumira reagent kuti isvike mukamuri tembiricha isati yashandiswa. Yakavhurwa reagent iri
yakagadzikana kwemwedzi mitanhatu pa6-2°C. |
| (ALEX²) Stop Solution | 1 bhodhoro kusvika 10 ml | Yakagadzirira kushandiswa. Chengetedza pa2-8°C kusvika zuva rekupera. Bvumira reagent kuti isvike mukamuri tembiricha isati yashandiswa. Yakavhurwa reagent yakagadzikana kwemwedzi mitanhatu pa6-2°C. Inogona kutaridzika senge turbid solution mushure mekuchengetedza kwenguva refu. Izvi hazvina zvazvinoita. |
ZVINO ZVINODIWA ZVOKUITA NEKUONGORORA
Manual Analysis
- ImageXplorer
- Arrayholder (kusarudza)
- Lab Rocker (kurerekera kona 8°, inodiwa kumhanya 8 rpm)
- Incubation chamber (WxDxH - 35x25x2 cm)
- RAPTOR SERVER Analysis Software
- PC/Laptop
Midziyo inodiwa, isina kupihwa neMADx:
- Demineralized Mvura
- Pipettes & matipi (100 µl & 100 - 1000 µl)
Automatic Analysis:
- MAX mudziyo (MAX 45k kana MAX 9k)
- Washing Solution (REF 00-5003-01)
- Stop Solution (REF 00-5007-01)
- RAPTOR SERVER Analysis Software
- PC/Laptop
Maintenance services maererano nemirairo yemugadziri.
KUBATA ARRAYS
Usabate array surface. Chero hurema hwepamusoro hunokonzerwa nekugomara kana kupinza zvinhu zvinogona kukanganisa kuverenga kwakaringana kwemhedzisiro. Usatore ALEX² mifananidzo isati yaoma zvachose (yakaoma pakamuri tembiricha).
YAMBIRO UYE CHENJERERO
- Zvinokurudzirwa kupfeka kudzivirira kwemaoko nemaziso pamwe nemajasi erabhoritari uye kutevedzera maitiro akanaka emurabhoritari pakugadzirira nekubata mareagents uye s.amples.
- Zvinoenderana nemaitiro akanaka emurabhoritari, zvinhu zvese zvinobva muvanhu zvinofanirwa kutariswa sezvingangotapurira utachiona uye zvinobatwa nematanho akafanana neanoitwa nemurwere.amples.
- ALEX² Sample Diluent uye Washing Solution ine sodium azide (<0.1%) sechinhu chinochengetedza uye inofanira kubatwa nehanya. Chengetedzo data sheet inowanikwa pakukumbira.
- Iyo (ALEX²) Stop Solution ine Ethylenediaminetetraacetic acid (EDTA)-Solution uye inofanira kubatwa nehungwaru. Chengetedzo data sheet inowanikwa pakukumbira.
- Yekushandisa in-vitro diagnostic chete. Kwete yekushandiswa kwemukati kana kunze muvanhu kana mhuka.
- Vashandi vakadzidziswa kudzidzira murabhoritari chete ndivo vanofanira kushandisa kiti ichi.
- Paunosvika, tarisa kit zvikamu zvekukuvara. Kana chimwe chezvikamu chikakuvadzwa (semuenzaniso mabhodhoro ebhafa), bata MADx (support@madx.com) kana muparidzi wenzvimbo yako. Usashandise zvakakanganisika kit zvikamu, sezvo kushandiswa kwavo kunogona kutungamirira kukushaya kit kuita.
- Usashandise reagents kupfuura mazuva avo ekupera.
- Usasanganise mareagents kubva kumabheti akasiyana.
ELISA PROCEDURE
Kugadzirira
Kugadzirira kwesampzvishoma: Serum kana plasma (heparin, citrate, kwete EDTA) sampkubva kune capillary kana venous ropa rinogona kushandiswa. Ropa samples inogona kuunganidzwa uchishandisa maitiro akajairwa. Chitoro sampzvishoma pa 2–8°C kusvika kwevhiki imwe. Chengetedza serum uye plasma sampyakaderera pa -20°C kuitira kuchengetedza kwenguva refu. Kutumirwa kweserum/plasma sampzvishoma pakupisa kwekamuri kunoshanda. Nguva dzose bvumira sampkuti isvike kamuri tembiricha isati yashandiswa.
Gadziriro yeWashing Solution (chete REF 02-5001-01 uye REF 00-5003-01 kana yashandiswa neMAX mudziyo): Dururira zviri mubhodhoro re1 reWashing Solution mumudziyo wekugezesa wechiridzwa. Zadza mvura ine demineralized kusvika kune yakatsvuka uye nyatso sanganisa mudziyo wacho kakawanda pasina kuburitsa furo. Iyo reagent yakavhurwa yakagadzikana kwemwedzi mitanhatu pa6-2 ° C.
Incubation chamber: Vhara chivharo kune ese matanho ekuedza kudzivirira kudonha kwehunyoro.
Parameters of Maitiro:
- 100µl pample + 400 µl ALEX² Sample Diluent
- 500 µl ALEX² Detection Antibody
- 500 µl ALEX² Substrate Solution
- 100 µl (ALEX²) Stop Solution
- 4500 µl Washing Solution
Nguva yeAssay ingangoita 3 h 30 min (pasina kuomeswa kweakagadziridzwa array).
Hazvikurudzirwe kumhanyisa maassays akawanda kupfuura anogona kubhowa mu8 min. Ese incubations inoitwa mukamuri tembiricha, 20-26 ° C.
Mareagents ese anofanirwa kushandiswa mukamuri tembiricha (20-26 ° C). Kuongorora hakufanirwe kuitwa muchiedza chezuva.
Gadzirira incubation chamber
Vhura incubation chamber woisa matauro epepa pazasi. Soak mapepa emapuranga nemvura yakasvibiswa kusvikira pasina zvikamu zvakaoma zvemapepa emapepa zvinoonekwa.
Sample incubation/CCD inhibition
Bvisa nhamba inodiwa yeALEX² cartridges uye uiise mune array holder (s). Wedzera 400 μl yeALEX² Sample Diluent kune imwe neimwe cartridge. Wedzera 100 μl murwere sample kuma cartridges. Iva nechokwadi chokuti mhinduro inoguma yakapararira zvakafanana. Isa ma cartridges mukamuri yakagadzirirwa incubation uye isa iyo incubation chamber nemakaturiji pa lab rocker kuitira kuti ma cartridges anozununguka parutivi rurefu rwe cartridge. Tanga serum incubation ne8 rpm kwemaawa maviri. Vhara incubation chamber usati watanga lab rocker. Mushure memaawa maviri, bvisa samples mumudziyo wekuunganidza. Nyatsopukuta madonhwe kubva pacartridge uchishandisa tauro repepa.
Dzivisa kubata array surface nepepa tauro! Dzivisa chero kutakurira kana kuyambuka-kusvibiswa kwesampzvishoma pakati pemunhu ALEX² cartridges!
Optional or positive Hom s LF (CCD marker): neyakajairwa CCD antibody inhibition protocol (sezvinotsanangurwa mundima 2: sample incubation / CCD inhibition) iyo CCD inhibition inoshanda i85%. Kana chiyero chepamusoro chekudzivisa kushanda chinodiwa, gadzirira 1 ml sample chubhu, wedzera 400 μl ALEX² Sample Diluent uye 100 μl serum. Chengetedza maminetsi makumi matatu (kwete-kuzunungusa) wozoenderera mberi nemaitiro akajairika ekuyedza.
Cherechedza: Iyo yakawedzera CCD inhibition nhanho inotungamirira muzviitiko zvakawanda kune inhibition rate yeCCD antibodies inopfuura 95%.
1a. Washing I
Wedzera 500 μl Washing Solution kune yega yega cartridge uye incuite pane lab rocker (pa8 rpm) kwemaminetsi mashanu. Bvisa iyo Washing Solution mumudziyo wekuunganidza uye urove nesimba ma cartridges pamurwi wemapepa akaoma. Nyatsopukuta madonhwe akasara kubva pamakaturiji uchishandisa tauro repepa.
Dzokorora danho iri 2 dzimwe nguva.
Wedzerai ma antibodies
Wedzera 500 µl yeALEX² Detection Antibody kune yega yega cartridge.
Ita shuwa kuti iyo yakazara array nzvimbo yakafukidzwa neALEX² Detection Antibody mhinduro.
Isa ma cartridges mukamuri yekufukidzira pane lab rocker uye incubate pa8 rpm kwemaminitsi makumi matatu. Bvisa mushonga weDetection Antibody mumudziyo wekuunganidza uye urove zvine simba makaturiji ari pamurwi wemapepa akaoma. Nyatsopukuta madonhwe akasara kubva pamakaturiji uchishandisa tauro repepa.
2a. Kugeza II
Wedzera 500 μl Washing Solution kune yega yega cartridge uye incuite pa lab rocker pa8 rpm kwemaminitsi mashanu. Bvisa iyo Washing Solution mumudziyo wekuunganidza uye urove nesimba ma cartridges pamurwi wemapepa akaoma. Nyatsopukuta madonhwe akasara kubva pamakaturiji uchishandisa tauro repepa.
Dzokorora danho iri 4 dzimwe nguva.
3+4. Wedzera ALEX² Substrate Solution uye misa substrate kuita
Wedzera 500 μl yeALEX² Substrate Solution kune yega yega cartridge. Tanga timer nekuzadza cartridge yekutanga uye enderera mberi nekuzadza kwekaturiji yakasara. Ita shuwa kuti iyo yakazara dhizaini yakafukidzwa neSubstrate Solution uye incubate arrays kwe8 maminetsi chaiwo pasina kuzununguka (lab rocker pa0 rpm uye munzvimbo yakachinjika).
Mushure memaminitsi masere chaiwo, wedzera 8 μl ye (ALEX²) Stop Solution kumakaturiji ese, kutanga nekaturiji yekutanga kuvimbisa kuti maarray ese akaiswa panguva imwe chete neALEX² Substrate Solution. Chengetedza kugovanisa zvakaenzana (ALEX²) Misa Solution mumakaturiji akarongwa, mushure mekunge (ALEX²) Stop Solution yaiswa pombi pane ese arrays. Mushure mezvo buditsa (ALEX²) Substrate/Stop Solution kubva kumakaturiji uye nekudzvanya zvine simba macartridges pamurwi wemapepa akaoma. Nyatsopukuta chero madonhwe akasara kubva pamakaturiji uchishandisa tauro repepa.
Iyo Lab Rocker haifanire KUZUNZA panguva ye substrate incubation!
4a. Kugeza III
Wedzera 500 μl Washing Solution kune yega yega cartridge uye incuite pa lab rocker pa8 rpm kwemasekonzi makumi matatu. Bvisa iyo Washing Solution mumudziyo wekuunganidza uye urove nesimba ma cartridges pamurwi wemapepa akaoma. Nyatsopukuta chero madonhwe akasara kubva pamakaturiji uchishandisa tauro repepa.
Kuongorora kwemifananidzo
Mushure mekupedza maitiro ekuongorora, mhepo omesa mitsara pakupisa kwekamuri kusvikira yaoma zvachose (inogona kutora kusvika ku45 min).
Kuomeswa kwakakwana kunokosha pakunzwa kwekuedzwa. Chete arrays akaomeswa zvakakwana anopa chiratidzo chakakwana kune ruzha reshiyo.
Chekupedzisira, mitsara yakaomeswa inoongororwa neImageXplorer kana MAX mudziyo woongororwa neRAPTOR SERVER Analysis software (ona zvakadzama muRAPTOR SERVER software handbook). Iyo RAPTOR SERVER Analysis Software inongosimbiswa pamwe chete neImageXplorer chiridzwa uye MAX zvishandiso, saka MADx haitore chero mutoro wemhedzisiro, iyo yakawanikwa neimwe imwe mudziyo wekutora mifananidzo (senge scanner).
Assay Calibration
Iyo ALEX² master calibration curve yakavambwa nereferensi yekuyedza kurwisa serum gadziriro ine chaiyo IgE kurwisa akasiyana maantigen anovhara iyo yaitarisirwa kuyerwa renji. Roti chaiyo calibration paramita inopihwa neRAPTOR SERVER Analysis Software. ALEX² sIgE mibairo yebvunzo inoratidzwa se kUA/l. Yese mibairo yeIgE ndeye semi-quantitative uye yakaverengerwa kubva kune anti-IgE kuyerwa ine mijenya-chaiyo calibration zvinhu, izvo zvinopihwa neRAPTOR SERVER Analysis Software uye inosarudzwa zvichienderana neiyo yakawanda-yakatarwa QR-makodhi.
Curve paramita yejeti yega yega inogadziriswa ne-in-house referensi yekuongorora system, inopesana neserum gadziriro yakaedzwa paImmunoCAP (Thermo Fisher Scientific) yeIgE chaiyo inopesana neakawanda allergenic. Iyo ALEX² mhedzisiro saka inoteedzera zvisina kunanga kutarisana neiyo WHO referensi gadziriro 11/234 yeIgE yakazara.
Misiyano yakarongeka mumatanho echiratidzo pakati pemijenya inogadziriswa neheterologous calibration inopesana neIgE referensi curve. Chinhu chekugadzirisa chinoshandiswa kugadzirisa zvine hungwaru kune mijenya-chaiyo kuyerwa kutsauka.
Measuring Range
Yakananga IgE: 0.3-50 kUA/l uwandu
Yese IgE: 20-2500 kU/l semi-quantitative
HUNHU KUDZORERA
Kuchengeta rekodhi kune imwe neimwe bvunzo
Zvinoenderana nemaitiro akanaka emurabhoritari zvinokurudzirwa kurekodha nhamba dzemajenya ese anoshandiswa.
Kudzora Sayenzi
Maererano nemaitiro akanaka erabhoritari zvinokurudzirwa kuti kutonga kwehutano sampLes inosanganisirwa mukati menguva dzakatsanangurwa. Reference values kune imwe yekutengeserana inowanikwa yekudzora sera inogona kupihwa neMADx pakukumbira.
DATA ANALYSIS
Pakuongororwa kwemifananidzo yeakagadziriswa arrays, iyo ImageXplorer kana MAX mudziyo unofanirwa kushandiswa. ALEX² mifananidzo inoongororwa otomatiki uchishandisa RAPTOR SERVER Analysis Software uye mushumo unogadzirwa uchipfupisa mibairo yemushandisi.
RESULTS
ALEX² ndeyehuwandu ELISA bvunzo kune chaiyo IgE uye semi-huwandu nzira yeIgE yakazara. Allergen-specific IgE masoja ekudzivirira chirwere anotaridzwa seIgE mhinduro maunitsi (kUA/l), ese eIgE mhinduro se kU/l. RAPTOR SERVER Analysis Software inoverengera otomatiki uye inoshuma sIgE zvawanikwa (muhuwandu) uye tIgE zvawanikwa (semi- quantitatively).
ZVIKAPIMIRIRA ZVINOITA
A definitive clinic diagnosis inofanira kungoitwa pamwe chete nezvose zvinowanikwa zvipatara zvinowanikwa nevashandi vezvokurapa uye hazvizobvi pamigumisiro yeimwe nzira yekuongorora chete.
Mune dzimwe nzvimbo dzekushandisa (semuenzaniso kusawirirana kwechikafu), masoja ekudzivirira chirwere anotenderera eIgE anogona kuramba asingaonekwe kunyangwe ratidziro yekiriniki yekusawirirana kwechikafu kune imwe allergen inogona kunge iripo, nekuti masoja ekudzivirira chirwere aya anogona kunge ari chaiwo kune aleji anogadziriswa panguva yekugadzira maindasitiri, kubika kana kugaya. uye nokudaro hazvipo pakudya kwepakutanga kunoongororwa murwere.
Mhedzisiro yehuturu husina kunaka hunongoratidza huwandu husingaonekwi hwehuturu hwemaantibodies eIgE (semuenzaniso nekuda kwekusaonekwa kwenguva refu) uye hazvitadzise kuvapo kwekiriniki hypersensitivity kune kurumwa netumbuyu.
Muvana, kunyanya kusvika kumakore maviri ezera, iyo yakajairika tIgE yakaderera pane yevechiri kuyaruka nevakuru [2]. Naizvozvo, zvinofanirwa kutarisirwa kuti muchikamu chepamusoro chevana vari pasi pemakore maviri iyo yakazara IgE-level iri pasi peiyo yakatarwa yekuona muganhu. Izvi zvinogumira hazvishande kune chaiyo IgE kuyerwa.
ZVINOTARISIRWA ZVINOKOSHA
Kushamwaridzana kwepedyo pakati pe-allergen-specific IgE antibody mazinga uye chirwere chechirwere chinozivikanwa uye chinotsanangurwa zvakakwana mumabhuku [1]. Murwere wega wega anonzwisiswa anoratidza munhu IgE profile kana yakaedzwa neALEX². Mhinduro yeIgE ine sampzvishoma kubva kune vane hutano vasiri-alergic vanhu vanozove pazasi 0.3 kUA/l kune imwe chete molecular allergens uye yeallergen extracts kana yakaedzwa neALEX². Nzvimbo yekutarisa yeIgE yakazara muvakuru ndeye <100 kU/l. Tsika yakanaka yemurabhoritari inokurudzira kuti rabhoritari yega yega imise huwandu hwayo hwehunhu hunotarisirwa.
PERFORMANCE CHARACTERISTICS
Maitiro ekuita pamwe neChidimbu cheKuchengetedza uye Kuita zvinogona kuwanikwa paMADx websaiti: https://www.madx.com/extras.
WARRANTY
Iyo data yekushanda yakawanikwa uchishandisa maitiro akatsanangurwa mune ino Mirayiridzo Yekushandisa. Chero shanduko kana kugadziridzwa mukuita kunogona kukanganisa zvabuda uye MacroArray Diagnostics inorambidza zvese waranti yakataurwa (kusanganisira inorehwa waranti yekutengesa uye kusimba kwekushandisa) muchiitiko chakadaro. Nekuda kweizvozvo, MacroArray Diagnostics nevashambadziri venzvimbo havazove nemhosva yekukuvadza zvisina kunanga kana kukonzeresa muchiitiko chakadaro.
MAZWIRO
| ALEX | Allergy Xplorer |
| CCD | Cross-reactive carbohydrate determinants |
| EDTA | Ethylenediaminetetraacetic acid |
| ELISA | Enzyme-Yakabatanidzwa Immunosorbent Assay |
| IgE | Immunoglobulin E |
| IVD | In-vitro diagnostic |
| kU/l | Makirogiramu paLita |
| kUA/l | Kilo mayuniti e-allergen-specific IgE parita |
| MADx | MacroArray Diagnostics |
| REF | Reference number |
| rpm | Round paminiti |
| sIgE | Allergen-specific IgE |
| tIgE | Total IgE |
| µl | Microliter |
ALLERGEN rondedzero ALEX²
Allergen zvinyorwa: Aca m, Aca s, Achd, Ail a, All c, All s, Amar, Amb a, Ana o, Api m, Art v, Ave s, Ber e, Bos d nyama, Bos d mukaka, Bro p , Cam d, Can f ♂ weti, Can s, Cap a, Cap h epithelia, Cap h mukaka, Car c, Car i, Car p, Che a, Che q, Chi spp., Cic a, Cit s, Cla h , Clu h, Cor a pollen, Cuc p, Cup s, Cyn d, Dau c, Dol spp., Equ c mukaka, Equ c nyama, Fag e, Fic b, Fic c, Fra e, Gad m, Gal d nyama , Gal d white, Gal d yolk, Hel a, Hom g, Hor v, Jug r, Jun a, Len c, Lit s, Loc m, Lol spp., Lup a, Mac i, Man i, Mel g, Mor r, Mus a, Myt e, Ori v, Ory nyama, Ory s, Ost e, Ovi a epithelia, Ovi nyama, Ovi a mukaka, Pan b, Pan m, Pap s, Par j, Pas n, Pec spp. , Pen ch, Per a, Pers a, Pet c, Pha v, Phr c, Pim a, Pis s, Pla l, Pold, Pop n, Pru av, Pru du, Pyr c, Raj c, Rat n, Rud spp., Sac c, Sal k, Sal s, Sco s, Sec c hupfu, Sec c mukume, Ses i, Sin, Sol spp., Sola l, Sol t, Sus d epithel, Sus d nyama, Gumi m, Thu a, Tri fo, Tri s, Tyr p, Ulm c, Urt d, Vac m, Ves v, Zea m hupfu
Yakanatswa yezvakasikwa zvinhu: nAct d 1, nApi m 1, nAra h 1, nAra 3, nBos d 4, nBos d 5, nBos d 6, nBos d 8, nCan f 3, nCor a 9, nCor a 11, nCup a 1, nCry j 1, nEqu c 3, nFag e 2, nGad m 1, nGad m 2 + 3, nGal d 2, nGal d 3, nGal d 4, nGal d 5, nGly m 5, nGly m 6, nJug r 4, nMac i 2S Albumin, nOle e 7 (RUO), nPap s 2S Albumin, nPis v 3, nPla a 2, nTri a aA_TI
Recombinant components: rAct d 10, rAct d 2, rAct d 5, rAln g 1, rAln g 4, rAlt a 1, rAlt a 6, rAmb a 1, rAmb a 4, rAna o 2, rAna o 3, rAni Ani s 1, rApi g 3, rApi g 1, rApi g 2, rApi m 6, rAra h 10, rAra h 2, rAra h 6, rAra h 8, rArg r 9, rArt v15 rAsp f 1, rAsp f 1, rAsp f 3, rAsp f 1, rBer e 3, rBet v 4, rBet v 6, rBet v 1, rBla g 1, rBla g 2, rBla g 6, rBla gla 1, 2, rBlo t 4, rBlo t 5, rBlo t 9, rBos d 10, rCan f 21, rCan f 5, rCan f 2, rCan f 1, rCan f Fel d 2 kufanana, rCan s 4, rCav p 6, a 1, rCla h 3, rClu h 1, rCor a 1, rCor a 8, rCor a 1, rCor a 1.0103 (RUO), rCor a 1.0401, rCra c 8, , rCuc m 12, rCyp 14 Cyp , rDau c 6, rDer f 2, rDer f 1, rDer p 1, rDer p 1, rDer p 1, rDer p 2, rDer p 1, rDer p 10, rDer p 11, rDer p 2, rDer p 20, c 21, rEqu c 23, rFag s 5, rFel d 7, rFel d 1, rFel d 4, rFel d 1, rFra a 1 + 2, rFra e 4, rGal d 7, rGly d m 1, rGly m 3, rHev b 1, rHev b 1, rHev b 2, rHev b 4, rHev b 8, rHev b 1, rHom s LF, rJug r 3, rJug r 5, rJug r 6.02, 8d r Lep 11r. , rLol p 1, rMal d 2, rMal d 3, rMala s 6, rMala s 2, rMala s 1, rMal d 1, rMer a 3, rMes a 11 (RUO), rMus m 5, rOle 6, rOry c 2, rOry c 1, rOry c 1, rPar j 1, rPen m 1, rPen m 9, rPen m 1, rPen m 2, rPer a 3, rPhl p 2, rPhl p 1, rPhl rPhl p 2, rPhl p 3, rPhl p 4, rPho d 7, rPhod s 1, rPis v 12, rPis v 2, rPis v 5.0101 (RUO), rPla a 6, rPla a 7, rPla 2 Pol 1, , rPru p 1, rPru p 2 (RUO), rRaj c Parvalbumin, rSal k 4, rSal s 1, rSco s 3, rSes i 1, rSin a 5, rSola l 3, rSus d 7, rTThuri a 1, 1, rTri a 1, rTyr p 1, rVes v 1, rVes v 6, rVit v 1, rXip g 1, rZea m 14
REFERENCES
- Hamilton, RG. (2008). Kuongororwa kwezvirwere zvevanhu. Clinical Immunology. 1471-1484. 10.1016/B978-0-323-04404-2.10100-9.
- Harwanegg C, Laffer S, Hiller R, Mueller MW, Kraft D, Spitzauer S, Valenta R. Microarrayed recombinant allergens yekuongororwa kweallergy. Clin Exp Allergy. 2003 Jan;33(1):7-13. doi: 10.1046/j.1365-2222.2003.01550.x. PMID: 12534543.
- Hiller R, Laffer S, Harwanegg C, Huber M, Schmidt WM, Twardosz A, Barletta B, Becker WM, Blaser K, Breiteneder H, Chapman M, Crameri R, Duchêne M, Ferreira F, Fiebig H, Hoffmann-Sommergruber K, King TP, Kleber-Janke T, Kurup VP, Lehrer SB, Lidholm J, Müller U, Pini C, Reese G, Scheiner O, Scheynius A, Shen HD, Spitzauer S, Suck R, Swoboda I, Thomas W, Tinghino R, Van Hage-Hamsten M, Virtanen T, Kraft D, Müller MW, Valenta R. Microarrayed allergenic molecules: diagnostic magedhi ekurapa allergy. FASEB J. 2002 Mar;16(3):414-6. doi: 10.1096/fj.01-0711fje. Epub 2002 Jan 14. PMID: 11790727
- Ferrer M, Sanz ML, Sastre J, Bartra J, del Cuvillo A, Montoro J, Jáuregui I, Dávila I, Mullol J, Valero A. Molecular diagnosis mu allergology: kushandiswa kwemaitiro e microarray. J Investig Allergol Clin Immunol. 2009;19 Suppl 1:19-24. PMID: 19476050.
- Ott H, Fölster-Holst R, Merk HF, Baron JM. Allergen microarrays: chinyorwa chinyorwa chepamusoro-resolution IgE profiling muvakuru vane atopic dermatitis. Eur J Dermatol. 2010 Jan-Kukadzi;20(1):54-
61. doi: 10.1684/ejd.2010.0810. Epub 2009 Oct 2. PMID: 19801343. - Sastre J. Molecular kuongororwa mune allergy. Clin Exp Allergy. 2010 Oct;40(10):1442-60. doi: 10.1111/j.1365-2222.2010.03585.x. Epub 2010 Aug 2. PMID: 20682003.
- Martins TB, Bandhauer ME, Bunker AM, Roberts WL, Hill HR. Hutsva hutsva uye nguva dzekutarisa dzevakuru dzeIgE yakazara. J Allergy Clin Immunol. 2014 Feb;133(2):589-91.
Kuti uwane ruzivo nezve zvakaitwa analytical uye zvekiriniki zvidzidzo tarisa kune maitiro ekuita pa https://www.madx.com/extras.
CHINJA NHOROONDO
| Version | Tsanangudzo | Inotsiva |
| 11 | nGal d1 yakashandurwa kuita rGal d1; URL yakagadziridzwa ku madx.com; CE yakawedzerwa nehuwandu hweMuviri Wakaziviswa; chinja nhoroondo yakawedzerwa | 10 |
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MacroArray Diagnostics
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MacroArray ALLERGY XPLORER Macro Array Diagnostics [pdf] Mirayiridzo 91201229202JQ, 02-2001-01, 02-5001-01, ALLERGY XPLORER Macro Array Diagnostics, ALLERGY XPLORER, Macro Array Diagnostics, Array Diagnostics, Diagnostics |
