MacroArray ALLERGY XPLORER Ntuziaka nyocha nchọpụta nnukwu ihe

Nọmba ntụaka: REF 02-2001-01, 02-5001-01
Eji Ezubere: Nchọpụta IgE (sIgE) kpọmkwem na-ahụ maka ihe nfụkasị ahụ na mkpokọta IgE (tIgE) ọkara n'otu n'otu.

Ndị ọrụ: Ndị ọrụ ụlọ nyocha a zụrụ azụ na ndị ọkachamara ahụike n'ụlọ nyocha ahụike
Nchekwa: Kit reagents kwụsiri ike maka ọnwa 6 ka emepechara

Ntuziaka ojiji ngwaahịa

Ụkpụrụ nke Usoro
Ngwaahịa a na-achọpụta IgE kpọmkwem ihe nfụkasị ahụ na mkpokọta IgE ọkara n'otu n'otu.

Mbupu na Nchekwa
Gbaa mbọ hụ na echekwara reagents ngwa dịka egosiri ma jiri ya mee ihe n'ime ọnwa 6 nke imeghe.

Mkpofu mkpofu:
Soro usoro mkpofu mkpofu kwesịrị ekwesị dịka iwu siri dị.

Ngwa ngwa
Rụtụ aka na ntuziaka onye ọrụ maka ozi zuru ezu na ngwa ngwa.

Ngwa achọrọ

Nyocha akwụkwọ ntuziaka: Gbaa mbọ hụ na ị nwere akụrụngwa dị mkpa nke onye nrụpụta nyere.

Nyocha akpaaka: Jiri ngwaọrụ MAX, Ngwọta ịsa ahụ, Ngwọta Kwụsị, Ngwa nyocha SERVER RAPTOR, yana PC/Laptọọpụ. Soro ntuziaka mmezi nke ọma.

Ijikwa nke Arrays
Soro ntuziaka enyere maka ijikwa nhazi nke ọma iji hụ na nsonaazụ ziri ezi.

Ịdọ aka ná ntị na ịkpachara anya

Yiri ngwa nchebe kwesịrị ekwesị dị ka mkpuchi aka na anya na uwe ụlọ nyocha.

Jikwaa reagents na samples eso ezi laabu omume.
Na-emeso ihe niile mmadụ si enweta dị ka ndị nwere ike ibute ọrịa ma jikwaa ya nke ọma.

FAQ

Ajụjụ: Ogologo oge ole ka reagents kit na-akwụsi ike?
A: Kit reagents kwụsiri ike maka ọnwa 6 ka emepere ya mgbe echekwara ya n'okpuru ọnọdụ egosiri.

Ajụjụ: Onye nwere ike iji ngwaahịa a?
A: Ezubere ngwaahịa a maka ndị ọrụ ụlọ nyocha a zụrụ azụ yana ndị ọkachamara ahụike n'ụlọ nyocha ahụike.

www.madx.com
Allergy XPLORER (ALEX²) Ntuziaka maka ojiji

Nkọwa

Allergy Xplorer (ALEX²) bụ Enzyme-Linked Immunosorbent Assay (ELISA) - dabere na in-vitro diagnostic ule maka ọnụọgụ ọnụọgụ nke IgE (sIgE) akọwapụtara.
Ntuziaka maka ojiji a na-adabara maka ngwaahịa ndị a:

UDI-DI bụ isi	REF	Ngwaahịa
91201229202JQ	02-2001-01	ALEX² maka nyocha 20
91201229202JQ	02-5001-01	ALEX² maka nyocha 50

Ebumnobi ebumnobi

ALEX² Allergy Xplorer bụ ngwa ule a na-eji maka nyocha in-vitro nke serum ma ọ bụ plasma mmadụ (ma e wezụga EDTA-plasma) iji nye ozi iji nyere aka chọpụta ndị ọrịa na-arịa ọrịa ogbugbo nke IgE na njikọ nchoputa ụlọ ọgwụ ndị ọzọ ma ọ bụ nsonaazụ nyocha nyocha. .
Ngwa ahụike nke IVD na-achọpụta IgE (sIgE) nke na-ahụ maka ihe nfụkasị ahụ n'ọnụ ọgụgụ yana ngụkọta IgE (tIgE) ọkara n'otu n'otu. Ndị ọrụ ụlọ nyocha a zụrụ azụ na ndị ọkachamara ahụike na-eji ngwaahịa a na ụlọ nyocha ahụike.

NKỌWA NA NKWAWA NKE Nnwale ahụ

Mmeghachi omume nfụkasị bụ ụdị mmeghachi omume hypersensitivity nke I ozugbo yana nje nje ndị sitere na klas IgE nke immunoglobulins na-ebute ya. Mgbe ekpughere ya na ihe nfụkasị ahụ kpọmkwem, mwepụta nke histamine na ndị ọzọ na-eme ka IgE na-esite na mkpụrụ ndụ mast na basophils na-ebute ngosipụta ahụike dịka ụkwara ume ọkụ, rhino-conjunctivitis nfụkasị, atopic eczema, na mgbaàmà eriri afọ [1]. Ya mere, usoro nleba anya zuru oke maka ndị na-ahụ maka ihe nfụkasị ahụ na-enyere aka na nyocha nke ndị ọrịa na-ahụ maka ihe nfụkasị ahụ [2-6]. Enweghị mmachi na ọnụ ọgụgụ ule. Mgbe ị na-etolite nyocha IgE, a naghị ewere afọ na mmekọahụ dị ka ihe dị oke egwu n'ihi na ọkwa IgE, nke a na-atụle na nyocha ndị a, adịghị agbanwe agbanwe na-adabere na ndị a.
ALEX² kpuchiri ụdị isi iyi I allergen niile. Enwere ike ịchọta ndepụta zuru oke nke ALEX² ihe nfụkasị ahụ na ihe nfụkasị molekụla n'okpuru ntuziaka a.

Ozi dị mkpa maka onye ọrụ!
Maka iji ALEX² nke ọma, ọ dị mkpa ka onye ọrụ jiri nlezianya gụọ ma soro ntuziaka ndị a maka ojiji. Onye nrụpụta anaghị ewere ụgwọ ọ bụla maka ojiji sistemụ nnwale a na-akọwaghị n'ime akwụkwọ a ma ọ bụ maka mgbanwe nke onye na-eji sistemụ nnwale a.
Ntị: variant kit 02-2001-01 nke ule ALEX² (20 Arrays) bụ naanị maka nhazi ntuziaka. Iji jiri ụdị ALEX² nke a nwere MAX 9k na-akpaghị aka, a ga-enye iwu ịsa Ngwọta (REF 00-5003-01) na Ngwọta Kwụsị (REF 00-5007-01) iche. Enwere ike ịchọta ozi ngwaahịa niile na ntuziaka dabara adaba maka ojiji: https://www.madx.com/extras.
Enwere ike iji ụdị ALEX² kit 02-5001-01 (50 arrays) maka nhazi akpaghị aka na ngwaọrụ MAX 9k (REF 17-0000-01) yana ngwaọrụ MAX 45k (REF 16-0000-01).

Ụkpụrụ nke usoro

ALEX² bụ nnwale immunoassay dabere na Enzyme-Linked Immunosorbent Assay (ELISA). A na-edobe ihe nfụkasị ahụ nke Allergen ma ọ bụ ihe nfụkasị ahụ molekụla, nke jikọtara ya na nanoparticles, n'usoro n'usoro n'usoro n'usoro siri ike na-akpụ usoro macroscopic. Nke mbụ, allergens na-ejikọta urughuru na-emeghachi omume na kpọmkwem IgE nke dị na s onye ọrịa.ample. Mgbe emechara ya, a na-asachapụ IgE na-abụghị nke akọwapụtara. Usoro a na-aga n'ihu site n'ịgbakwunye ihe mgbochi nchọpụta IgE nke akpọrọ mgbochi mmadụ nke na-etolite mgbagwoju anya nke nwere kpọmkwem IgE. Mgbe usoro ịsa ahụ nke abụọ gasịrị, a na-agbakwunye mkpụrụ nke na-agbanwe ka ọ bụrụ ihe na-adịghị edozi ahụ, nke nwere agba site na enzyme na-egbochi mmadụ. N'ikpeazụ, a na-akwụsị mmeghachi omume enzyme-substrate site n'ịgbakwunye reagent na-egbochi ya. Ọnụọgụ mmiri ozuzo dabara na ntinye nke kpọmkwem IgE na s onye ọrịaample. Usoro nyocha ụlọ nyocha na-esote site na inweta na nyocha ihe onyonyo site na iji usoro ntuziaka (ImageXplorer) ma ọ bụ sistemụ akpaaka (MAX 45k ma ọ bụ MAX 9k). A na-enyocha nsonaazụ ule ahụ site na RAPTOR SERVER Analysis Software wee kọwa ya na nkeji nzaghachi IgE (kUA/l). A na-akọkwa ngụkọta nsonaazụ IgE na nkeji nzaghachi IgE (kU/l). SERVER RAPTOR dị na ụdị 1, maka ọnụọgụ ọnụọgụ ọnụọgụ anọ zuru ezu biko rụtụ aka na akara RAPTOR SERVER dị na www.raptor-server.com/imprint.

Mbupu na nchekwa
Mbupu ALEX² na-ewere ọnọdụ n'ọnọdụ ọnọdụ okpomọkụ. Agbanyeghị, a ga-echekwa ngwa ahụ ozugbo ebufere ya na 2-8 Celsius. Echekwara nke ọma, ALEX² na ihe mejupụtara ya nwere ike iji ruo ụbọchị njedebe egosipụtara.

Kit reagents kwụsiri ike maka ọnwa 6 ka emepechara (na ọnọdụ nchekwa egosiri).

Mkpofu ihe mkpofu
Tụfuo katrij ALEX² eji na ngwa ngwa ejighi ya nwere mkpofu kemịkalụ laabu. Soro ụkpụrụ mba, steeti na mpaghara gbasara mkpofu.

Nkọwa nkọwa

Ngwakọta ngwa
Akụkụ nke ọ bụla (reagent) kwụsiri ike ruo ụbọchị ekwuru na akara akụrụngwa nke ọ bụla. A naghị atụ aro ka ikpokọta reagents ọ bụla sitere na nza ngwa dị iche iche. Maka ndepụta nke ihe nfụkasị nfụkasị ahụ na ihe nfụkasị ahụ molekụla anaghị emegharị n'usoro ALEX², biko kpọtụrụ nkwado@madx.com.

Ngwa ngwa REF 02-2001-01	Ọdịnaya	Njirimara
ALEX² Katrij	2 Blisters a 10 ALEX² maka nyocha 20 na mkpokọta. Calibration site na isi usoro dị site na RAPTOR SERVER Ngwanrọ nyocha.	Njikere maka ojiji. Chekwaa na 2-8 Celsius C ruo ụbọchị njedebe.
ALEX² Sampna diluent	1 karama na 9 ml	Njikere maka ojiji. Chekwaa na 2-8 Celsius C ruo ụbọchị njedebe. Kwe ka reagent ruo ụlọ okpomọkụ tupu eji ya. Reagent mepere emepe kwụsiri ike maka ọnwa 6 na 2-8 Celsius, gụnyere ihe mgbochi CCD.
Ngwọta ịsa ahụ	2 karama na 50 ml	Njikere maka ojiji. Chekwaa na 2-8 Celsius C ruo ụbọchị njedebe. Kwe ka reagent ruo ụlọ okpomọkụ tupu eji ya. Reagent mepere emepe kwụsiri ike maka ọnwa 6 na 2-8 Celsius.

Ngwa ngwa REF 02-2001-01	Ọdịnaya	Njirimara
ALEX² Antibody	1 karama na 11 ml	Njikere maka ojiji. Chekwaa na 2-8 Celsius C ruo ụbọchị njedebe. Kwe ka reagent ruo ụlọ okpomọkụ tupu eji ya. Reagent mepere emepe kwụsiri ike maka ọnwa 6 na 2-8 Celsius.
ALEX² ngwọta ngwọta	1 karama na 11 ml	Njikere maka ojiji. Chekwaa na 2-8 Celsius C ruo ụbọchị njedebe. Kwe ka reagent ruo ụlọ okpomọkụ tupu eji ya. Reagent mepere emepe kwụsiri ike maka ọnwa 6 na 2-8 Celsius.
(ALEX²) Kwụsị Ngwọta	1 karama na 2.4 ml	Njikere maka ojiji. Chekwaa na 2-8 Celsius C ruo ụbọchị njedebe. Kwe ka reagent ruo ụlọ okpomọkụ tupu eji ya. Reagent mepere emepe kwụsiri ike maka ọnwa 6 na 2-8 Celsius. Nwere ike ịpụta dị ka ihe ngwọta turbid mgbe nchekwa ogologo gasịrị. Nke a enweghị mmetụta na nsonaazụ.

Ngwa ngwa REF 02-5001-01	Ọdịnaya	Njirimara
ALEX² Katrij	5 Blisters a 10 ALEX² maka nyocha 50 na mkpokọta. Calibration site na usoro ukwu dị site na RAPTOR SERVER Analysis Software.	Njikere maka ojiji. Chekwaa na 2-8 Celsius C ruo ụbọchị njedebe.
ALEX² Sampna diluent	1 karama na 30 ml	Njikere maka ojiji. Chekwaa na 2-8 Celsius C ruo ụbọchị njedebe. Kwe ka reagent ruo ụlọ okpomọkụ tupu eji ya. Reagent mepere emepe kwụsiri ike maka ọnwa 6 na 2-8 Celsius, gụnyere ihe mgbochi CCD.
Ngwọta ịsa ahụ	4 x nkwe. 1 karama na 250 ml	Chekwaa na 2-8 Celsius C ruo ụbọchị njedebe. Ghichaa 1 ruo 4 na mmiri demineralized tupu eji ya. Kwe ka reagent ruo ụlọ okpomọkụ tupu eji ya. Reagent mepere emepe kwụsiri ike maka ọnwa 6 na 2-8 Celsius.
ALEX² Antibody	1 karama na 30 ml	Njikere maka ojiji. Chekwaa na 2-8 Celsius C ruo ụbọchị njedebe. Kwe ka reagent ruo ụlọ okpomọkụ tupu eji ya. Reagent mepere emepe kwụsiri ike maka ọnwa 6 na 2-8 Celsius.

Ngwa ngwa REF 02-5001-01	Ọdịnaya	Njirimara
ALEX² ngwọta ngwọta	1 karama na 30 ml	Njikere maka ojiji. Chekwaa na 2-8 Celsius C ruo ụbọchị njedebe. Kwe ka reagent ruo ụlọ okpomọkụ tupu eji ya. Mepere reagent bụ kwụsie ike maka ọnwa 6 na 2-8 Celsius.
(ALEX²) Kwụsị Ngwọta	1 karama na 10 ml	Njikere maka ojiji. Chekwaa na 2-8 Celsius C ruo ụbọchị njedebe. Kwe ka reagent ruo ụlọ okpomọkụ tupu eji ya. Reagent mepere emepe kwụsiri ike maka ọnwa 6 na 2-8 Celsius. Nwere ike ịpụta dị ka ihe ngwọta turbid mgbe nchekwa ogologo gasịrị. Nke a enweghị mmetụta na nsonaazụ.

Akụrụngwa achọrọ maka nhazi na nyocha

Nyocha akwụkwọ ntuziaka

ImageXplorer
Onye njide (nhọrọ)
Lab Rocker (ntụgharị uche 8 Celsius, ọsọ achọrọ 8 rpm)
Ụlọ nnabata (WxDxH - 35x25x2 cm)
Ngwanrọ nyocha ihe nkesa RAPTOR
PC/Laptọọpụ

Akụrụngwa achọrọ, nke MADx enyeghị ya:

Mmiri nke Demineralized
Pipettes na ndụmọdụ (100 µl & 100-1000 µl)

Nyocha akpaaka:

Ngwaọrụ MAX (MAX 45k ma ọ bụ MAX 9k)
Ngwọta ịsacha (REF 00-5003-01)
Ngwọta Kwụsị (REF 00-5007-01)
Ngwanrọ nyocha ihe nkesa RAPTOR

PC/Laptọọpụ

Ọrụ nlekọta dịka ntuziaka ndị nrụpụta siri dị.

Ijikwa ARRAYS

Emetụla elu n'usoro. Nrụrụ elu ọ bụla nke ihe na-adịghị nkọ ma ọ bụ nkọ kpatara nwere ike igbochi agụpụta nsonaazụ ya. Enwetala ihe onyonyo ALEX² tupu nhazi ahụ akpọnwụọ kpamkpam (akpọọ nkụ na ụlọ okpomọkụ).

Ịdọ aka ná ntị na ịkpachara anya

A na-atụ aro iyi mkpuchi aka na anya yana uwe lab ma soro usoro ụlọ nyocha dị mma mgbe ị na-akwadebe ma na-ejikwa reagents na s.amples.
N'ikwekọ na omume ụlọ nyocha dị mma, a ga-ewere ihe niile mmadụ si enweta dị ka ọrịa na-efe efe ma jiri otu nlezianya mee ya dịka onye ọrịa.amples.
ALEX² Sample Diluent na Ngwọta ịsa nwere sodium azide (<0.1%) dị ka ihe nchekwa na a ga-ejikwa nlezianya mee ya. Mpempe data nchekwa dị mgbe a rịọrọ ya.

Ngwọta Nkwụsị (ALEX²) nwere Ethylenediaminetetraacetic acid (EDTA) - ngwọta na a ga-ejikwa nlezianya mee ya. Mpempe data nchekwa dị mgbe a rịọrọ ya.
Maka iji nyocha in-vitro naanị. Ọ bụghị maka ime ma ọ bụ mpụga ojiji mmadụ ma ọ bụ anụmanụ.
Naanị ndị ọrụ zụrụ azụ n'ọrụ ụlọ nyocha kwesịrị iji ngwa a.

Mgbe ị bịarutere, lelee akụrụngwa akụrụngwa maka mmebi. Ọ bụrụ na otu n'ime ihe ndị ahụ mebiri emebi (dịka karama nchekwa), kpọtụrụ MADx (nkwado@madx.com) ma ọ bụ onye nkesa mpaghara gị. Ejila ngwa ngwa mebiri emebi, n'ihi na ojiji ha nwere ike ibute arụmọrụ ngwa adịghị mma.
Ejila reagents gafere ụbọchị njedebe ha.
Ejikọtala reagents sitere na batches dị iche iche.

Usoro ELISA

Nkwadebe
Nkwadebe nke samples: Serum ma ọ bụ plasma (heparin, citrate, enweghị EDTA) sampEnwere ike iji les sitere na capillary ma ọ bụ venous ọbara. Ọbara sampEnwere ike ịnakọta les site na iji usoro ọkọlọtọ. Ụlọ ahịa sampna-erughị 2-8 Celsius C ruo otu izu. Debe serum na plasma sampna-erughị -20 Celsius C maka nchekwa ogologo oge. Mbupu nke serum/plasma samples na ọnụ ụlọ okpomọkụ dị. Kwe ka samples iru ụlọ okpomọkụ tupu eji.
Nkwadebe nke Ngwọta Ngwọta (naanị maka REF 02-5001-01 na REF 00-5003-01 mgbe ejiri ngwaọrụ MAX): Wunye ọdịnaya nke 1 vial nke Ngwọta Ngwọta n'ime akpa ịsa ahụ. Jupụta mmiri demineralized ruo akara uhie ma jiri nlezianya gwakọta akpa ahụ ọtụtụ oge n'ebughị ụfụfụ. Reagent mepere emepe kwụsiri ike maka ọnwa 6 na 2-8 Celsius.
Ụlọ ntinye: Mechie mkpuchi maka usoro nyocha niile iji gbochie ọdịda iru mmiri.

Oke of Usoro:

100 µl sample + 400 µl ALEX² Sampna diluent
Ihe mgbochi nchọpụta 500 µl ALEX²
500 µl ALEX² ihe ngwọta

100 µl (ALEX²) Kwụsị Ngwọta
Ngwọta ịsa 4500 µl

Oge nyocha bụ ihe dịka awa 3 30 nkeji (na-enweghị ihicha nke nhazi nhazi).
A naghị atụ aro ka ị na-agba ọsọ nyocha karịa ka enwere ike ịgbanye na 8 min. A na-eme incubations niile na ụlọ okpomọkụ, 20-26 Celsius C.

A ga-eji reagents niile na okpomọkụ nke ụlọ (20-26 Celsius C). Agaghị eme nyocha ahụ na ìhè anyanwụ.

Kwadebe ụlọ incubation
Mepee ụlọ incubation ma tinye akwa nhicha akwụkwọ n'akụkụ ala. Tinye akwa nhicha akwụkwọ na mmiri demineralized ruo mgbe a na-ahụghị akụkụ akọrọ nke akwa akwa akwụkwọ.

Sample incubation / CCD mgbochi
Wepụta ọnụọgụ ALEX² dị mkpa ma tinye ha n'ime ihe njide nhazi. Tinye 400 μl ALEX² Sample Diluent ọ bụla katrij. Tinye 100 μl onye ọrịaample ka cartridges. Gbaa mbọ hụ na a na-agbasa ihe ngwọta nke ga-esi na ya pụta. Debe cartridges n'ime ime ụlọ a kwadebere ma tinye ebe a na-emepụta ihe na cartridges na ụlọ nyocha nke mere na cartridges na-ama jijiji n'akụkụ ogologo nke katrij. Malite incubation serum na 8 rpm maka awa 2. Mechie ọnụ ụlọ nnabata tupu ịmalite rocker lab. Mgbe awa 2 gachara, hapụ samples n'ime akpa nchịkọta. Jiri nlezianya hichapụ ụmụ irighiri mmiri na katrijị site na iji akwa nhicha akwụkwọ.

Zere iji towel akwụkwọ metụ elu ahụ dị n'usoro! Zenarị ihe ọ bụla ebufe ma ọ bụ mmetọ nke samples n'etiti katrij ALEX² n'otu n'otu!

Nhọrọ ma ọ bụ nke ziri ezi Hom s LF (CCD markker): na ọkọlọtọ CCD mgbochi mgbochi protocol (dị ka akọwara na paragraf 2: sample incubation / CCD mgbochi) arụmọrụ mgbochi CCD bụ 85%. Ọ bụrụ na achọrọ oke mmachi mgbochi, kwadebe 1 ml sample tube, tinye 400 μl ALEX² Sample Diluent na 100 μl serum. Tinyegharịa maka nkeji 30 (anaghị ama jijiji) wee gaa n'ihu na usoro nyocha.
Mara: Mgbakwunye mgbochi CCD na-eduga n'ọtụtụ ọnọdụ na ọnụego mgbochi maka ọgwụ mgbochi CCD karịrị 95%.

1a. Ịsa I
Tinye 500 μl Ngwọta ịsa na katrij ọ bụla ma tinye ya na rocker lab (na 8 rpm) maka nkeji 5. Wepụ ihe ngwọta ịsa ahụ n'ime akpa nnakọta wee kpatụ katrijị ndị ahụ n'ụkọ akwa akwa akwa kpọrọ nkụ. Jiri nlezianya hichapụ ụmụ irighiri mmiri ndị fọdụrụ na katrijị site na iji akwa nhicha akwụkwọ.
Tinyegharịa nzọụkwụ a ugboro abụọ ọzọ.

Tinye ọgwụ mgbochi nchọpụta
Tinye 500 µl nke ALEX² Antibody Detection na katrij ọ bụla.

Gbaa mbọ hụ na ihe ngwọta ALEX² kpuchiri elu n'usoro zuru ezu.

Tinye katrijị ahụ n'ime ụlọ ntinye na ụlọ nyocha wee tinye ya na 8 rpm maka nkeji 30. Wepụ ihe mgbochi nchọpụta nchọpụta n'ime akpa nnakọta wee kpatụ katrijị ndị ahụ n'ụkọ akwa akwa akwa kpọrọ nkụ. Jiri nlezianya hichapụ ụmụ irighiri mmiri ndị fọdụrụ na katrijị site na iji akwa nhicha akwụkwọ.

2a. Ịsacha II
Tinye 500 μl Ngwọta ịsa na katrij ọ bụla ma tinye ya na rocker lab na 8 rpm maka nkeji ise. Wepụ ihe ngwọta ịsa ahụ n'ime akpa nnakọta wee kpatụ katrijị ndị ahụ n'ụkọ akwa akwa akwa kpọrọ nkụ. Jiri nlezianya hichapụ ụmụ irighiri mmiri ndị fọdụrụ na katrijị site na iji akwa nhicha akwụkwọ.
Tinyegharịa nzọụkwụ a ugboro abụọ ọzọ.

3+4. Tinye ALEX² Substrate Solution ma kwụsị mmeghachi omume mkpụrụ osisi
Tinye 500 μl ALEX² Substrate Solution na katrij ọ bụla. Malite ngụ oge na-ejuputa katrij nke mbụ wee gaa n'ihu na ndochi nke katrij ndị fọdụrụ. Jide n'aka na ihe ngwọta kpuchiri elu ahụ n'usoro zuru ezu ma tinye usoro ahụ maka nkeji 8 kpọmkwem na-enweghị ịma jijiji (lab rocker na 0 rpm na n'ọnọdụ kwụ ọtọ).
Mgbe nkeji 8 gachara, tinye 100 μl nke (ALEX²) Ngwọta Kwụsị na katrij niile, malite na katrij nke mbụ iji jide n'aka na a na-etinye usoro niile n'otu oge na ngwọta ALEX². Jiri nlezianya kpalie ikesa (ALEX²) Ngwọta Kwụsị n'ime katrij n'usoro, mgbe (ALEX²) Kwụsị Ngwọta na-agbanye n'usoro niile. Mgbe nke ahụ gasịrị, wepụ ihe ngwọta (ALEX²) / Kwụsị Ngwọta n'ime katrijị wee kpatụ katrijị ahụ ike n'elu akwa akwa akwa akwa. Jiri nlezianya hichapụ ụmụ irighiri mmiri ndị fọdụrụ na katrijị site na iji akwa nhicha akwụkwọ.

Lab Rocker agaghị ama jijiji n'oge ntinye mkpụrụ!

4a. Ịsacha III
Tinye 500 μl Ngwọta ịsa na katrij ọ bụla ma tinye ya na rocker lab na 8 rpm maka sekọnd 30. Wepụ ihe ngwọta ịsa ahụ n'ime akpa nnakọta wee kpatụ katrijị ndị ahụ n'ụkọ akwa akwa akwa kpọrọ nkụ. Jiri nlezianya hichapụ ụmụ irighiri mmiri ndị fọdụrụ na katrijị site na iji akwa nhicha akwụkwọ.

Nyocha onyonyo
Mgbe ịmechara usoro nyocha ahụ, kpoo arrays ndị ahụ n'ime ụlọ okpomọkụ ruo mgbe ha kpochara kpamkpam (nwere ike were ihe dị ka 45 min).

Nhicha zuru oke dị mkpa maka uche nke ule. Naanị usoro akpọnwụ akpọnwụ kpamkpam na-enye akara kacha mma maka oke mkpọtụ.

N'ikpeazụ, a na-eji ImageXplorer ma ọ bụ ngwaọrụ MAX nyochaa usoro ndị a mịrị amị ma jiri ngwa nyocha RAPTOR SERVER nyocha (lee nkọwapụta na akwụkwọ ntuziaka RAPTOR SERVER). A na-enyocha sọftụwia nyocha nke SERVER naanị yana yana ngwa ImageXplorer na ngwaọrụ MAX, yabụ MADx anaghị eburu ọrụ ọ bụla maka nsonaazụ, nke enwetara na ngwaọrụ njide onyonyo ọ bụla (dị ka nyocha).

Nhazi nhazi

Ewubere usoro nhazi nhazi nke ALEX² site na nyocha ntụle megide nkwadebe serum nwere IgE kpọmkwem megide antigens dị iche iche na-ekpuchi oke nha. Ngwanrọ nyocha RAPTOR SERVER na-enye parampat nhazi nhazi ọtụtụ. A na-akọwapụta nsonaazụ ule ALEX² sIgE dị ka kUA/l. Ngụkọta nsonaazụ IgE bụ ọkara ọnụọgụ wee gbakọọ site na nleba mgbochi IgE nwere ihe nrụzi ọtụtụ, nke RAPTOR SERVER Analysis Software weputara wee họrọ dabere na koodu QR-kpọmkwem.
A na-ahazi usoro nleba anya n'ime ụlọ maka nza ọ bụla, megide nkwadebe ọbara nke a nwalere na ImmunoCAP (Thermo Fisher Scientific) maka kpọmkwem IgE megide ọtụtụ allergens. Ya mere, a na-achọpụta nsonaazụ ALEX² na-apụtaghị ìhè megide nkwadebe ntụaka WHO 11/234 maka mkpokọta IgE.
Ọdịiche dị n'usoro n'ọkwa mgbaama n'etiti nza na-ahazi ya site na nhazigharị heteroloji megide akụkụ ntụaka IgE. A na-eji ihe mgbazi na-ahazi n'usoro maka ndahie nha nha kpọmkwem.

Oke nso
Specific IgE: 0.3-50 kUA/l nha
Mkpokọta IgE: 20-2500 kU/l ọkara ọnụọgụ

Njikwa ogo

Idekọ ndekọ maka nyocha ọ bụla
Dị ka ezigbo ụlọ nyocha omume ọ na-atụ aro ka ịdekọ nza nọmba nke niile reagents eji.

Ụdị njikwa
Dị ka ezigbo ụlọ nyocha a na-atụ aro ka njikwa mma sampA na-etinye les n'ime oge akọwapụtara. Enwere ike ịnye ụkpụrụ ntụnye aka maka ụfọdụ sera njikwa azụmaahịa dị site na MADx ma rịọ ya.

Nyocha data

Maka nyocha onyonyo nke usoro nhazi, ImageXplorer ma ọ bụ ngwaọrụ MAX ga-eji. A na-enyocha onyonyo ALEX² ozugbo site na iji RAPTOR SERVER Analysis Software wee wepụta akụkọ na-achịkọta nsonaazụ maka onye ọrụ.

Nsonaazụ
ALEX² bụ ule ELISA ọnụ maka kpọmkwem IgE na usoro ọnụọgụ ọnụọgụ maka mkpokọta IgE. A na-akọwapụta ọgwụ mgbochi IgE kpọmkwem dị ka nkeji nzaghachi IgE (kUA/l), ngụkọta IgE dị ka kU/l. RAPTOR SERVER Analysis Software na-agbakọ na-akpaghị aka ma na-akọ rịzọlt sIgE (n'ụzọ dị iche iche) na tIgE (n'obere quantitatively).

MKWU NDỊ NA-ECHICHE

A ga-eme nyocha ụlọ ọgwụ zuru oke na njikọta yana nyocha ụlọ ọgwụ niile dị site n'aka ndị ọkachamara ahụike agaghị adabere na nsonaazụ nke otu usoro nyocha naanị.
N'akụkụ ụfọdụ nke ngwa (dịka ihe nfụkasị nri), nje nje IgE na-ekesa nwere ike ịnọgide na-adịghị achọpụta ya n'agbanyeghị na ngosipụta ụlọ ọgwụ nke ihe nfụkasị nri megide ụfọdụ allergen nwere ike ịdị, n'ihi na ọgwụ nje ndị a nwere ike bụrụ kpọmkwem maka allergens nke na-agbanwe n'oge nhazi ụlọ ọrụ, nri ma ọ bụ mgbaze. ya mere adịghị adị na nri mbụ nke a na-anwale onye ọrịa.
Nsonaazụ venom na-adịghị mma na-egosi naanị ọkwa a na-achọpụtaghị nke ọgwụ mgbochi IgE kpọmkwem (dịka n'ihi ogologo oge anaghị ekpughe ya) yana anaghị egbochi ịdị adị nke hypersensitivity nke ụlọ ọgwụ na nsị ahụhụ.
N'ime ụmụaka, ọkachasị ndị ruru afọ abụọ, ọnụọgụ tIgE nkịtị dị ala karịa ndị nọ n'afọ iri na ụma na ndị okenye [2]. Ya mere, a ga-atụ anya na n'ime ọnụ ọgụgụ dị elu nke ụmụaka na-erubeghị afọ 7 ngụkọta ọkwa IgE dị n'okpuru njedebe nchọpụta akọwapụtara. Mmachi a anaghị emetụta nha IgE akọwapụtara.

Ụkpụrụ echere
Mmekọrịta chiri anya dị n'etiti ọkwa mgbochi IgE nke allergen-kpọmkwem na ọrịa nfụkasị bụ nke a maara nke ọma ma kọwaa ya nke ọma na akwụkwọ [1]. Onye ọrịa ọ bụla nwere uche ga-egosi onye IgE profile mgbe a nwalere ya na ALEX². Nzaghachi IgE na samples sitere na ndị nwere ahụike na-adịghị ahụ efe ga-adị n'okpuru 0.3 kUA/l maka otu allergens molekụla yana maka mmịpụta allergen mgbe a nwalere ya na ALEX². Ebe ntụaka maka ngụkọta IgE na ndị okenye bụ <100 kU/l. Omume ụlọ nyocha dị mma na-atụ aro ka ụlọ nyocha ọ bụla guzobe oke nke ụkpụrụ a tụrụ anya ya.

ÀJỤMỤ ỌRỤ
Enwere ike ịchọta njirimara arụmọrụ yana nchịkọta nke Safety na Performance na MADx websaịtị: https://www.madx.com/extras.

NKWUKWU

Enwetara data arụmọrụ site na iji usoro akọwapụtara na ntuziaka maka ojiji a. Mgbanwe ọ bụla ma ọ bụ mgbanwe ọ bụla na usoro a nwere ike imetụta nsonaazụ ya yana MacroArray Diagnostics na-ekwupụta ikike niile egosipụtara (gụnyere akwụkwọ ikike nke ahịa na mma maka ojiji) na mmemme dị otú ahụ. N'ihi ya, MacroArray Diagnostics na ndị na-ekesa mpaghara agaghị enwe ụgwọ maka mmebi na-apụtaghị ìhè ma ọ bụ ihe ga-esi na ya pụta na mmemme dị otú ahụ.

MKPEBI

ALEX	Allergy Xplorer
CCD	Ndị na-achọpụta carbohydrate na-emeghachi omume
EDTA	Ethylenediaminetetraacetic acid
ELISA	Enzyme-njikọ Immunosorbent Assay
IgE	Immunoglobulin E
IVD	Nchọpụta n'ime vitro
ku/l	Kilo nkeji kwa lita
kUA/l	Otu kilo nke IgE kpọmkwem ihe nfụkasị ahụ kwa lita
MADx	Ihe nchọpụta MacroArray
REF	Nọmba ntụaka
rpm	Gburugburu kwa nkeji
sIgE	IgE kpọmkwem-allergen
tIgE	Mkpokọta IgE
µl	Microlite

Ndepụta Allergen ALEX²

Allergen wepụ: Aca m, Aca s, Ach d, Ail a, All c, All s, Ama r, Amb a, Ana o, Api m, Art v, Ave s, Ber e, Bos d anụ, Bos d mmiri ara ehi, Bro p , Cam d, Nwere ike f ♂ mmamịrị, Nwere ike s, Cap a, Cap h epithelia, Cap h milk, Car c, Car i, Car p, Che a, Che q, Chi spp., Cic a, Cit s, Cla h , Clu h, Cor a pollen, Cuc p, Cup s, Cyn d, Dau c, Dol spp., Equ c mmiri ara ehi, Equ c anụ, Fag e, Fic b, Fic c, Fra e, Gad m, Gal d anụ. , Gal d ọcha, Gal d nkochi ime akwa, Hel a, Hom g, Hor v, Jug r, Jun a, Len c, Lit s, Loc m, Lol spp., Lup a, Mac i, Man i, Mel g, Mor r, Mus a, Myt e, Ori v, Anụ ory, Ory s, Ost e, Ovi a epithelia, Ovi anụ, Ovi a mmiri ara, Pan b, Pan m, Pap s, Par j, Pas n, Pec spp. , Pen ch, Per a, Pers a, Pet c, Pha v, Phr c, Pim a, Pis s, Pla l, Pold, Pop n, Pru av, Pru du, Pyr c, Raj c, Rat n, Rud spp., Sac c, Sal k, Sal s, Sco s, ntụ ọka nke abụọ, Sec c pollen, Ses i, Sin, Sol spp., Sola l, Sol t, Sus d epithel, Sus d anụ, Iri m, Thu a, Tri fo, Tri s, Tyr p, Ulm c, Urt d, Vac m, Ves v, Zea m ntụ ọka

Akụkụ eke emechara ọcha: nAct d 1, nApi m 1, nAra h 1, nAra h 3, nBos d 4, nBos d 5, nBos d 6, nBos d 8, nCan f 3, nCor a 9, nCor a 11, nCup a 1, nCry j. 1, nEqu c 3, nFag e 2, nGad m 1, nGad m 2 + 3, nGal d 2, nGal d 3, nGal d 4, nGal d 5, nGly m 5, nGly m 6, nJug r 4, nMac i. 2S Albumin, nOle e 7 (RUO), nPap s 2S Albumin, nPis v 3, nPla a 2, nTri na aA_TI

Ngwakọta ejikọta: rAct d 10, rAct d 2, rAct d 5, rAln g 1, rAln g 4, rAlt a 1, rAlt a 6, rAmb a 1, rAmb a 4, rAna o 2, rAna o 3, rAnir s 1. 3, rApi g 1, rApi g 2, rApi g 6, rApi m 10, rAra h 2, rAra h 6, rAra h 8, rAra h 9, rAra h 15, rArg r 1, rArt v 1, 3 r, A. rAsp f 1, rAsp f 3, rAsp f 4, rAsp f 6, rBer e 1, rBet v 1, rBet v 2, rBet v 6, rBla g 1, rBla g 2, rBla g 4, rBla g 5, 9, rBlo t 10, rBlo t 21, rBlo t 5, rBos d 2, rCan f 1, rCan f 2, rCan f 4, rCan f 6, rCan f Fel d 1 dị ka, rCan s 3, rCav p 1. a 1, rCla h 8, rClu h 1, rCor a 1.0103, rCor a 1.0401, rCor a 8, rCor a 12 (RUO), rCor a 14, rCra c 6, , rCuc m 2, 1pryn , rDau c 1, rDer f 1, rDer f 1, rDer p 2, rDer p 1, rDer p 10, rDer p 11, rDer p 2, rDer p 20, rDer p 21, rDer p 23, rDer p 5, 7r. c 1, rEqu c 4, rFag s 1, rFel d 1, rFel d 2, rFel d 4, rFel d 7, rFra a 1 + 3, rFra e 1, rGal d 1, rGly d 2, rGly m 4, m 8, rHev b 1, rHev b 3, rHev b 5, rHev b 6.02, rHev b 8, rHev b 11, rHom s LF, rJug r 1, rJug r 2, rJug r 3, 6 Jurp. , rLol p 2, rMal d 1, rMal d 1, rMala s 3, rMala s 11, rMala s 5, rMal d 6, rMer a 2, rMes a 1 (RUO), rMus m 1, rOle e 1. 1, rOry c 9, rOry c 1, rOry c 2, rPar j 3, rPen m 2, rPen m 1, rPen m 2, rPen m 3, rPer a 4, rPhl p 7, rPhl p 1, rPhl rPhl p 12, rPhl p 2, rPhl p 5.0101, rPho d 6, rPhod s 7, rPis v 2, rPis v 1, rPis v 1 (RUO), rPla a 2, rPla a 4, rPla l 1, , rPru p 3, rPru p 1 (RUO), rRaj c Parvalbumin, rSal k 5, rSal s 3, rSco s 7, rSes i 1, rSin a 1, rSola l 1, rSus d 1, rThu a 1, 6, rTri a 1, rTyr p 1, rVes v 14, rVes v 19, rVit v 2, rXip g 1, rZea m 5

Ntụaka

Hamilton, RG. (2008). Ntụle nke ọrịa nfụkasị mmadụ. Immunology Clinical. 1471-1484. 10.1016/B978-0-323-04404-2.10100-9.
Harwanegg C, Laffer S, Hiller R, Mueller MW, Kraft D, Spitzauer S, Valenta R. Microarrayed recombinant allergens maka ịchọpụta ihe nfụkasị ahụ. Clin Exp Allergy. 2003 Jan; 33 (1): 7-13 . doi: 10.1046/j.1365-2222.2003.01550.x. PMID: 12534543.

Hiller R, Laffer S, Harwanegg C, Huber M, Schmidt WM, Twardosz A, Barletta B, Becker WM, Blaser K, Breiteneder H, Chapman M, Crameri R, Duchêne M, Ferreira F, Fiebig H, Hoffmann-Sommergruber K, King TP, Kleber-Janke T, Kurup VP, Lehrer SB, Lidholm J, Müller U, Pini C, Reese G, Scheiner O, Scheynius A, Shen HD, Spitzauer S, Suck R, Swoboda I, Thomas W. Van Hage-Hamsten M, Virtanen T, Kraft D, Müller MW, Valenta R. Ụmụ irighiri ihe nfụkasị ahụ nke microarrayed: ndị nche ọnụ ụzọ nyocha maka ọgwụgwọ nrịanrịa. FASEB J. 2002 Mar; 16 (3): 414-6 . doi: 10.1096/fj.01-0711fje. Epub 2002 Jan 14. PMID: 11790727
Ferrer M, Sanz ML, Sastre J, Bartra J, del Cuvillo A, Montoro J, Jáuregui I, Dávila I, Mullol J, Valero A. Nchọpụta molecular na allergology: ngwa nke usoro microarray. J Nyochaa Allergol Clin Immunol. 2009;19 Ntụkwasị 1:19-24 . PMID: 19476050.
Ott H, Fölster-Holst R, Merk HF, Baron JM. Allergen microarrays: ngwá ọrụ ọhụrụ maka profaịlụ IgE dị elu na ndị okenye nwere atopic dermatitis. Eur J Dermatol. 2010 Jan-Feb;20(1):54-
61. doi: 10.1684/ejd.2010.0810. Epub 2009 Ọkt 2. PMID: 19801343.

Sastre J. Nchọpụta molecular na allergies. Clin Exp Allergy. 2010 Ọkt; 40 (10): 1442-60 . doi: 10.1111/j.1365-2222.2010.03585.x. Epub 2010 Ọgọst 2. PMID: 20682003.
Martins TB, Bandhauer ME, Bunker AM, Roberts WL, Hill HR. Oge ntụaka nwata na okenye ọhụrụ maka mkpokọta IgE. J Allergy Clin Immunol. 2014 Feb; 133 (2): 589-91 .

Maka nkọwa gbasara nyocha nyocha na nyocha ụlọ ọgwụ na-arụtụ aka na njirimara arụmọrụ na https://www.madx.com/extras.

GBANWEE Akụkọ

Ụdị	Nkọwa	Dochie
11	nGal d1 gbanwere rGal d1; URL emelitere ka madx.com; CE gbakwunyere na ọnụọgụ nke Òtù Amụma; agbakwunyere akụkọ mgbanwe	10

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Akwụkwọ / akụrụngwa

MacroArray Allergy XPLORER Macro Array Diagnostics [pdf] Ntuziaka
91201229202JQ.

Ntụaka

Akwụkwọ ntuziaka onye ọrụ

MacroArray Allergy XPLORER Macro Array Diagnostics