MacroArray ALLERGY XPLORER Umarnin Bincike na Macro Array

Masu amfani: Horarrun ma'aikatan dakin gwaje-gwaje da kwararrun likitoci a dakin gwaje-gwaje na likita
Ajiye: Kit ɗin reagents sun tsaya tsayin daka na tsawon watanni 6 bayan buɗewa

Umarnin Amfani da samfur

Ka'idar Tsarin
Samfurin yana gano takamaiman IgE-allergen a ƙididdigewa da jimillar IgE rabin-girma.

Shigo da Ajiya
Tabbatar an adana reagents kamar yadda aka nuna kuma ana amfani dasu cikin watanni 6 da buɗewa.

Sharar gida:
Bi hanyoyin zubar da shara kamar yadda ka'idoji suka tanada.

Abubuwan Kit
Koma zuwa littafin mai amfani don cikakkun bayanai akan abubuwan da aka haɗa kayan aiki.

Kayayyakin da ake buƙata

Binciken Manual: Tabbatar cewa kuna da kayan aikin da ake buƙata daga masana'anta.

Nazari ta atomatik: Yi amfani da na'urar MAX, Maganin Wankewa, Magani Tsaida, RAPTOR SERVER Analysis Software, da PC/Laptop. Bi umarnin kulawa a hankali.

Gudanar da Arrays
Bi umarnin da aka bayar don sarrafa tsararraki a hankali don tabbatar da ingantaccen sakamako.

Gargadi da Hattara

Saka kayan kariya masu dacewa kamar kariya ta hannu da ido da riguna na lab.

Sarrafa reagents da sampkasa bin kyawawan ayyukan dakin gwaje-gwaje.
Kula da duk kayan tushen ɗan adam a matsayin masu iya kamuwa da cuta kuma a kula dasu.

FAQ

Tambaya: Yaya tsawon lokacin da kayan aikin reagents ke dawwama?
A: Kit reagents sun tsaya tsayin daka na tsawon watanni 6 bayan buɗewa lokacin da aka adana su a ƙarƙashin yanayin da aka nuna.

Tambaya: Wanene zai iya amfani da wannan samfurin?
A: Wannan samfurin an yi nufin amfani da shi ta ƙwararrun ma'aikatan dakin gwaje-gwaje da ƙwararrun likita a cikin saitin dakin gwaje-gwaje na likita.

www.madx.com
ALLERGY XPLORER (ALEX²) UMARNIN AMFANI

BAYANI

Allergy Xplorer (ALEX²) wani Enzyme-Linked Immunosorbent Assay (ELISA) - tushen in-vitro diagnostic gwaje-gwaje don ƙididdige ma'auni na takamaiman IgE (sIgE).
Wannan umarnin don amfani yana aiki don samfuran masu zuwa:

Tushen UDI-DI	REF	Samfura
91201229202JQ	02-2001-01	ALEX² don Nazari 20
91201229202JQ	02-5001-01	ALEX² don Nazari 50

MANUFAR NUFIN

ALEX² Allergy Xplorer kayan gwaji ne da aka yi amfani da shi don gwajin in-vitro na jini ko plasma (ban da EDTA-plasma) don ba da bayanai don taimaka wa marasa lafiya da ke fama da cututtukan da ke cikin IgE tare da sauran binciken asibiti ko sakamakon gwajin gwaji. .
Na'urar likita ta IVD tana gano takamaiman IgE (sIgE) mai ƙididdigewa da jimillar IgE (tIgE) rabin-girma. ƙwararrun ma'aikatan dakin gwaje-gwaje da ƙwararrun likita ne ke amfani da samfurin a cikin dakin gwaje-gwaje na likita.

TAKAITACCE DA BAYANIN GWAJI

Halayen rashin lafiyar nau'in I kai tsaye halayen halayen haɓakawa kuma ana yin sulhu ta hanyar ƙwayoyin rigakafi na rukunin IgE na immunoglobulins. Bayan bayyanar cututtuka na musamman, sakin histamine na IgE da sauran masu shiga tsakani daga kwayoyin mast da basophils suna haifar da bayyanar cututtuka irin su asma, rashin lafiyar rhino-conjunctivitis, atopic eczema, da alamun gastrointestinal [1]. Sabili da haka, cikakken tsarin wayar da kan jama'a ga ƙayyadaddun allergens yana taimakawa wajen kimanta marasa lafiya [2-6]. Babu ƙuntatawa akan yawan gwajin. Lokacin haɓaka ƙididdigar IgE, yawancin shekaru da jima'i ba a la'akari da su azaman mahimman dalilai saboda matakan IgE, waɗanda aka auna a cikin waɗannan ƙididdigar, ba sa bambanta sosai dangane da waɗannan ƙididdigar.
Dukkanin manyan nau'ikan allergen I suna rufe ta ALEX². Ana iya samun cikakken jerin abubuwan da aka cire ALEX² allergens da allergens na ƙwayoyin cuta a ƙasan wannan umarni.

Bayani mai mahimmanci ga mai amfani!
Don daidaitaccen amfani da ALEX², ya zama dole ga mai amfani ya karanta a hankali kuma ya bi waɗannan umarnin don amfani. Mai sana'anta ba shi da wani alhaki ga kowane amfani da wannan tsarin gwajin wanda ba a bayyana shi a cikin wannan takarda ba ko don gyare-gyare ta mai amfani da tsarin gwajin.
Hankali: Bambancin kit 02-2001-01 na gwajin ALEX² (20 Arrays) an yi niyya ne kawai don sarrafa hannu. Don amfani da wannan bambance-bambancen kit na ALEX² tare da MAX 9k mai sarrafa kansa, Maganin Wanke (REF 00-5003-01) da Magani Tsaya (REF 00-5007-01) suna buƙatar yin oda daban. Ana iya samun duk ƙarin bayanan samfurin a cikin umarnin da suka dace don amfani: https://www.madx.com/extras.
Za a iya amfani da bambancin kit ɗin ALEX² 02-5001-01 (tsari 50) don sarrafawa ta atomatik tare da na'urar MAX 9k (REF 17-0000-01) da kuma na'urar MAX 45k (REF 16-0000-01).

KA'IDAR HANYA

ALEX² gwajin immunoassay ne akan Enzyme-Linked Immunosorbent Assay (ELISA). Allergens tsantsa ko kwayoyin allergens, waɗanda aka haɗe zuwa nanoparticles, ana ajiye su a cikin tsari na tsari akan wani lokaci mai ƙarfi da ke samar da tsararrun macroscopic. Na farko, allergens da ke daure suna amsawa tare da takamaiman IgE wanda ke cikin s na majiyyaci.ample. Bayan shiryawa, ana wanke IgE maras takamaiman. Ana ci gaba da hanyar ta ƙara wani ƙwayar cuta mai suna anti-dan adam IgE ganowa wanda ke samar da hadaddun tare da takamaiman IgE mai ɗaure. Bayan mataki na wankewa na biyu, ana ƙara ɓangarorin da ke jujjuya su zuwa yanayin da ba a iya narkewa, mai launin hazo ta hanyar enzyme da ke daure da jiki. A ƙarshe, ana dakatar da halayen enzyme-substrate ta ƙara reagent mai toshewa. Adadin hazo ya yi daidai da ƙaddamar da takamaiman IgE a cikin s ɗin mara lafiyaample. Ana bin tsarin gwajin gwaji ta hanyar siyan hoto da bincike ta amfani da tsarin jagora (ImageXplorer) ko tsarin sarrafa kansa (MAX 45k ko MAX 9k). Ana nazarin sakamakon gwajin tare da RAPTOR SERVER Analysis Software kuma an ba da rahoto a cikin sassan amsawar IgE (kUA/l). Hakanan ana ba da rahoton jimlar sakamakon IgE a cikin raka'o'in amsa IgE (kU/l). RAPTOR SERVER yana samuwa a cikin sigar 1, don cikakken lambar sigar lamba huɗu don Allah a duba tambarin SERVER na RAPTOR da ke akwai a www.raptor-server.com/imprint.

KASUWA DA AJIYA
Jirgin ALEX² yana faruwa ne a yanayin zafin yanayi. Duk da haka, dole ne a adana kit ɗin nan da nan bayan bayarwa a zazzabi na 2-8 ° C. An adana shi daidai, ALEX² da kayan aikin sa za a iya amfani da su har zuwa ranar da aka nuna.

Kit ɗin reagents sun tsaya tsayin daka na tsawon watanni 6 bayan buɗewa (a yanayin da aka nuna).

HARKAR SHArar gida
Zubar da harsashin ALEX² da aka yi amfani da shi da kayan aikin da ba a yi amfani da su ba tare da sharar sinadarai na dakin gwaje-gwaje. Bi duk dokokin ƙasa, jiha, da na gida dangane da zubarwa.

GLOSSARY OF ALAMOMIN

KIT ABUBUWAN
Kowane bangare (reagent) yana da ƙarfi har zuwa ranar da aka bayyana akan lakabin kowane ɓangaren ɓangaren. Ba a ba da shawarar tara kowane reagents daga kuri'a na kit daban-daban ba. Don jerin abubuwan da aka cire daga allergens da allergens na kwayoyin da ba a iya motsi a kan jerin ALEX², da fatan za a tuntuɓi support@madx.com.

Abubuwan Kit na REF 02-2001-01	Abun ciki	Kayayyaki
ALEX² Cartridge	2 Blisters a 10 ALEX² don nazarin 20 gabaɗaya. Calibration ta hanyar babban lanƙwasa akwai ta RAPTOR SERVER Software na Analysis.	Shirye don amfani. Ajiye a 2-8 ° C har sai ranar karewa.
ALEX² Sampda Diluent	1 kwalban a 9 ml	Shirye don amfani. Ajiye a 2-8 ° C har sai ranar karewa. Bada reagent don isa zafin dakin kafin amfani. Bude reagent yana da ƙarfi na tsawon watanni 6 a 2-8°C, ya haɗa da mai hana CCD.
Maganin Wanka	2 kwalban a 50 ml	Shirye don amfani. Ajiye a 2-8 ° C har sai ranar karewa. Bada reagent don isa zafin dakin kafin amfani. Bude reagent yana da kwanciyar hankali na tsawon watanni 6 a 2-8 ° C.

Abubuwan Kit na REF 02-2001-01	Abun ciki	Kayayyaki
ALEX² Maganin Ganewa	1 kwalban a 11 ml	Shirye don amfani. Ajiye a 2-8 ° C har sai ranar karewa. Bada reagent don isa zafin dakin kafin amfani. Bude reagent yana da kwanciyar hankali na tsawon watanni 6 a 2-8 ° C.
ALEX² Maganin Substrate	1 kwalban a 11 ml	Shirye don amfani. Ajiye a 2-8 ° C har sai ranar karewa. Bada reagent don isa zafin dakin kafin amfani. Bude reagent yana da kwanciyar hankali na tsawon watanni 6 a 2-8 ° C.
(ALEX²) Tsaida Magani	1 kwalban a 2.4 ml	Shirye don amfani. Ajiye a 2-8 ° C har sai ranar karewa. Bada reagent don isa zafin dakin kafin amfani. Bude reagent yana da kwanciyar hankali na tsawon watanni 6 a 2-8 ° C. Zai iya bayyana azaman maganin turbid bayan dogon ajiya. Wannan ba shi da wani tasiri akan sakamako.

Abubuwan Kit na REF 02-5001-01	Abun ciki	Kayayyaki
ALEX² Cartridge	5 Blisters a 10 ALEX² don nazarin 50 gabaɗaya. Calibration ta hanyar babban lanƙwasa akwai ta RAPTOR SERVER Analysis Software.	Shirye don amfani. Ajiye a 2-8 ° C har sai ranar karewa.
ALEX² Sampda Diluent	1 kwalban a 30 ml	Shirye don amfani. Ajiye a 2-8 ° C har sai ranar karewa. Bada reagent don isa zafin dakin kafin amfani. Bude reagent yana da ƙarfi na tsawon watanni 6 a 2-8°C, ya haɗa da mai hana CCD.
Maganin Wanka	4 x cin. 1 kwalban a 250 ml	Ajiye a 2-8 ° C har sai ranar karewa. Tsarma 1 zuwa 4 tare da ruwa mai lalacewa kafin amfani. Bada reagent don isa zafin dakin kafin amfani. Bude reagent yana da kwanciyar hankali na tsawon watanni 6 a 2-8 ° C.
ALEX² Maganin Ganewa	1 kwalban a 30 ml	Shirye don amfani. Ajiye a 2-8 ° C har sai ranar karewa. Bada reagent don isa zafin dakin kafin amfani. Bude reagent yana da kwanciyar hankali na tsawon watanni 6 a 2-8 ° C.

Abubuwan Kit na REF 02-5001-01	Abun ciki	Kayayyaki
ALEX² Maganin Substrate	1 kwalban a 30 ml	Shirye don amfani. Ajiye a 2-8 ° C har sai ranar karewa. Bada reagent don isa zafin dakin kafin amfani. Bude reagent shine barga na watanni 6 a 2-8 ° C.
(ALEX²) Tsaida Magani	1 kwalban a 10 ml	Shirye don amfani. Ajiye a 2-8 ° C har sai ranar karewa. Bada reagent don isa zafin dakin kafin amfani. Bude reagent yana da kwanciyar hankali na tsawon watanni 6 a 2-8 ° C. Zai iya bayyana azaman maganin turbid bayan dogon ajiya. Wannan ba shi da wani tasiri akan sakamako.

KAYAN ABUBUWAN DA AKE BUKATA DOMIN TSARKI DA BINCIKE

Nazarin Manual

ImageXplorer
Arrayholder (na zaɓi)
Lab Rocker (kusurwar karkata 8°, saurin da ake buƙata 8rpm)
Wurin shigar da ciki (WxDxH - 35x25x2 cm)
RAPTOR SERVER Analysis Software
PC/Laptop

Kayan aiki da ake buƙata, ba a samar da su ta MADx ba:

Ruwan da aka Rage
Pipettes & tukwici (100 µl & 100 - 1000 µl)

Nazari ta atomatik:

Na'urar MAX (MAX 45k ko MAX 9k)
Maganin Wanke (REF 00-5003-01)
Magani Tsaida (REF 00-5007-01)
RAPTOR SERVER Analysis Software

PC/Laptop

Ayyukan kulawa bisa ga umarnin masana'anta.

HANYAR ARZIKI

Kar a taɓa saman tsararru. Duk wata lahani da ke haifar da abubuwa masu kaifi ko kaifi na iya tsoma baki tare da daidaitaccen karatun sakamakon. Kar a siyi hotunan ALEX² kafin tsararru ta bushe gaba daya (bushe a zazzabi).

GARGADI DA TSIRA

Ana ba da shawarar sanya kariya ta hannu da ido da riguna na lab da kuma bin kyawawan ayyukan dakin gwaje-gwaje yayin shiryawa da sarrafa reagents da s.amples.
Dangane da kyakkyawan aikin dakin gwaje-gwaje, duk abubuwan tushen ɗan adam yakamata a yi la'akari da su na iya kamuwa da cuta kuma a kula dasu tare da taka tsantsan kamar yadda masu haƙuri.amples.
ALEX² SampLe Diluent da Maganin Wanke sun ƙunshi sodium azide (<0.1%) a matsayin abin kiyayewa kuma dole ne a kula da shi da kulawa. Ana samun takardar bayanan aminci akan buƙata.

Magani Tsaida (ALEX²) ya ƙunshi Ethylenediaminetetraacetic acid (EDTA) - Magani kuma dole ne a sarrafa shi da kulawa. Ana samun takardar bayanan aminci akan buƙata.
Don bincikar in-vitro amfani kawai. Ba don amfani na ciki ko na waje a cikin mutane ko dabbobi ba.
Ma'aikatan da aka horar da su a aikin gwaje-gwaje ne kawai ya kamata su yi amfani da wannan kit ɗin.

Bayan isowa, duba abubuwan kayan aikin don lalacewa. Idan ɗayan abubuwan da aka gyara ya lalace (misali kwalaben buffer), tuntuɓi MADx (support@madx.com) ko mai rabawa na gida. Kada a yi amfani da abubuwan da aka lalata kayan aikin, saboda amfanin su na iya haifar da rashin aikin kit ɗin.
Kada a yi amfani da reagents fiye da kwanakin ƙarewar su.
Kada a haxa reagents daga batches daban-daban.

HANYAR ELISA

Shiri
Shiri na samples: jini ko plasma (heparin, citrate, babu EDTA) sampAna iya amfani da les daga capillary ko venous jini. Jini samples za a iya tattara ta amfani da daidaitattun hanyoyin. Store sampLes a 2-8 ° C har zuwa mako guda. Rike jini da plasma sampLes a -20 ° C don dogon ajiya. Jirgin jini/plasma samples a dakin da zafin jiki ya dace. Izinin sampdon isa ga zafin jiki kafin amfani.
Shirye-shiryen Maganin Wanki (kawai don REF 02-5001-01 da REF 00-5003-01 lokacin amfani da na'urar MAX): Zuba abun ciki na 1 vial na Maganin Wankewa a cikin kwandon wanka na kayan aiki. Cika ruwan da aka lalatar da shi har zuwa alamar ja kuma a haɗe kwandon a hankali sau da yawa ba tare da samar da kumfa ba. Reagent da aka buɗe yana da ƙarfi na tsawon watanni 6 a 2-8 ° C.
Gidan haɓakawa: Rufe murfi don duk matakan tantancewa don hana faɗuwar zafi.

Siga of Tsari:

100 ml sample + 400 µl ALEX² Sampda Diluent
500 µl ALEX² Gano Maganin Cutar
500 µl ALEX² Maganin Substrate

100 µl (ALEX²) Magani Tsaida
Maganin Wanke 4500 µl

Lokacin tantancewa yana kusan 3 h 30 min (ba tare da bushewar tsararrun da aka sarrafa ba).
Ba a ba da shawarar yin gwaje-gwaje fiye da yadda za a iya yin bututu a cikin mintuna 8 ba. Ana yin duk incubations a dakin da zafin jiki, 20-26 ° C.

Dole ne a yi amfani da duk reagents a zafin jiki (20-26 ° C). Kada a yi gwajin a cikin hasken rana kai tsaye.

Shirya ɗakin shiryawa
Bude ɗakin shiryawa kuma sanya tawul ɗin takarda a ɓangaren ƙasa. Jiƙa tawul ɗin takarda da ruwa mai lalacewa har sai ba a ga busassun sassan tawul ɗin takarda ba.

Sample incubation/ hana CCD
Fitar da adadin da ake buƙata na akwatunan ALEX² kuma sanya su cikin ma'auni(s). Ƙara 400 μl na ALEX² Sample Diluent ga kowane harsashi. Ƙara 100 μl mai haƙuriample ga katun. Tabbatar cewa an yada sakamakon da aka samu a ko'ina. Sanya harsashi a cikin ɗakin da aka shirya da kuma sanya ɗakin shiryawa tare da harsashi a kan maƙallan lab don harsashi suna girgiza tare da dogon gefen harsashi. Fara shiryawa na magani tare da 8 rpm na awanni 2. Rufe ɗakin shiryawa kafin fara wasan rocker. Bayan awanni 2, fitar da samples a cikin akwati mai tarin yawa. A hankali goge ɗigon ruwa daga harsashi ta amfani da tawul ɗin takarda.

Ka guji taɓa saman jeri tare da tawul ɗin takarda! Guji duk wani abin ɗaukar kaya ko gurɓatawar samples tsakanin guda ALEX² harsashi!

Na zaɓi ko tabbatacce Hom s LF (alamar CCD): tare da daidaitattun ka'idar hana hanawar CCD ta antibody (kamar yadda aka bayyana a sakin layi na 2: sample incubation / hanawar CCD) ingancin hanawar CCD shine 85%. Idan ana buƙatar mafi girman ƙimar haɓakar hanawa, shirya 1 ml sample tube, ƙara 400 μl ALEX² Sample Diluent da 100 μl ruwan magani. Sanya na tsawon mintuna 30 (ba girgiza ba) sannan a ci gaba da tsarin tantancewa da aka saba.
Lura: Ƙarin matakin hana CCD yana kaiwa a lokuta da yawa zuwa ƙimar hanawa ga ƙwayoyin rigakafin CCD sama da 95%.

1 a. Wankan I
Ƙara 500 μl Maganin Wankewa a kowace harsashi kuma a saka a kan rocker (a 8 rpm) na minti 5. Zubar da Maganin Wankewa a cikin akwati mai tarin yawa kuma da ƙarfi tatsa kwas ɗin akan tarin busassun tawul ɗin takarda. A hankali a goge ragowar ɗigon ruwa daga cikin harsashi ta amfani da tawul ɗin takarda.
Maimaita wannan mataki sau 2.

Ƙara maganin rigakafi
Ƙara 500 µl na ALEX² Gano Antibody zuwa kowane harsashi.

Tabbatar cewa an rufe cikakkiyar farfajiyar ta ALEX² Gano Antibody bayani.

Sanya cartridges a cikin ɗakin shiryawa a kan rocker da kuma sanya shi a 8 rpm na minti 30. Zubar da Maganin Gano Maganin Ganewa a cikin akwati mai tarin yawa kuma da ƙarfi taɓa kwas ɗin akan tarin busassun tawul ɗin takarda. A hankali a goge ragowar ɗigon ruwa daga cikin harsashi ta amfani da tawul ɗin takarda.

2 a ba. Wankewa II
Ƙara 500 μl Maganin Wankewa a kowace harsashi kuma a sanya shi a kan rocker a 8 rpm na minti 5. Zubar da Maganin Wankewa a cikin akwati mai tarin yawa kuma da ƙarfi tatsa kwas ɗin akan tarin busassun tawul ɗin takarda. A hankali a goge ragowar ɗigon ruwa daga cikin harsashi ta amfani da tawul ɗin takarda.
Maimaita wannan mataki sau 4.

3+4. Ƙara ALEX² Maganin Substrate kuma dakatar da amsawar substrate
Ƙara 500 μl na ALEX² Substrate Solution zuwa kowane harsashi. Fara mai ƙidayar lokaci tare da cika harsashi na farko kuma ci gaba da cika sauran harsashi. Tabbatar cewa an rufe saman gabaɗaya ta hanyar Maganin Substrate kuma a sanya jeri na tsawon mintuna 8 daidai ba tare da girgiza ba (lab rocker a 0 rpm kuma a cikin matsayi a kwance).
Bayan daidai minti 8, ƙara 100 μl na (ALEX²) Magani Tsaida zuwa duk harsashi, farawa da harsashi na farko don tabbatar da cewa duk tsararru an haɗa su lokaci guda tare da ALEX² Substrate Solution. A hankali a tayar da hankali don rarraba Magani Tsaida (ALEX²) a cikin tsararrun harsashi, bayan (ALEX²) Maganin Tsaida da aka busa akan duk jeri. Bayan haka fitar da (ALEX²) Magani/Dakatar da Magani daga cikin harsashi kuma da ƙarfi taɓo harsashi akan tarin busassun tawul ɗin takarda. A hankali goge duk wasu ɗigon digo daga cikin harsashi ta amfani da tawul ɗin takarda.

Lab Rocker ba dole ba ne ya girgiza a lokacin shiryawa da wuri!

4a ba. Wankewa III
Ƙara 500 μl Maganin Wankewa a kowace harsashi kuma a sanya shi a kan rocker a 8 rpm na 30 seconds. Zubar da Maganin Wankewa a cikin akwati mai tarin yawa kuma da ƙarfi tatsa kwas ɗin akan tarin busassun tawul ɗin takarda. A hankali goge duk wasu ɗigon digo daga cikin harsashi ta amfani da tawul ɗin takarda.

Binciken hoto
Bayan kammala aikin tantancewa, iska ta bushe aruwan a dakin da zafin jiki har sai sun bushe gaba daya (zai iya ɗaukar har zuwa 45 min).

Cikakken bushewa yana da mahimmanci don jin daɗin gwajin. Busassun jeri-jeri ne kawai ke ba da ingantacciyar sigina zuwa rabon amo.

A ƙarshe, ana bincika busassun tsararrun tare da na'urar ImageXplorer ko na'urar MAX kuma ana bincikar su tare da software na Binciken SERVER na RAPTOR (duba cikakkun bayanai a cikin littafin Jagorar SERVER na RAPTOR). RAPTOR SERVER Analysis Software ana tabbatarwa ne kawai a hade tare da kayan aikin ImageXplorer da na'urorin MAX, don haka MADx baya ɗaukar wani nauyi don sakamako, waɗanda aka samu tare da kowace na'urar ɗaukar hoto (kamar na'urar daukar hoto).

Ƙididdigar Assay

ALEX² master calibration curve an kafa shi ta hanyar gwajin tunani game da shirye-shiryen magani tare da takamaiman IgE akan antigens daban-daban waɗanda ke rufe kewayon aunawa. Ƙimar ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙira an samar da su ta RAPTOR SERVER Analysis Software. Ana bayyana sakamakon gwajin ALEX² sIgE a matsayin kUA/l. Jimlar sakamakon IgE masu ƙididdigewa ne kuma an ƙididdige su daga ma'aunin anti-IgE tare da takamaiman abubuwan daidaitawa, waɗanda RAPTOR SERVER Analysis Software ke bayarwa kuma aka zaɓa bisa ga takamaiman takamaiman QR-codes.
Ana daidaita sigogin lanƙwasa don kowane ƙuri'a ta tsarin gwajin tunani na cikin gida, akan shirye-shiryen magani da aka gwada akan ImmunoCAP (Thermo Fisher Scientific) don takamaiman IgE akan allergens da yawa. Sakamakon ALEX² ana iya gano shi a kaikaice akan shirin WHO 11/234 don jimlar IgE.
Bambance-bambancen tsari a cikin matakan sigina tsakanin kuri'a an daidaita su ta hanyar daidaitawa iri-iri a kan madaidaicin IgE. Ana amfani da ma'aunin gyara don daidaitawa da tsari don ƙayyadaddun ma'auni na musamman.

Ma'auni Range
Specific IgE: 0.3-50 kUA/l ƙididdiga
Jimlar IgE: 20-2500 kU/l Semi-quantitative

KYAUTATA KYAUTA

Ajiye rikodi don kowane gwaji
Dangane da kyakkyawan aikin dakin gwaje-gwaje ana bada shawarar yin rikodin lambobi na duk reagents da aka yi amfani da su.

Samfuran sarrafawa
Dangane da kyakkyawan aikin dakin gwaje-gwaje ana bada shawarar cewa kula da ingancin samples an haɗa su cikin ƙayyadaddun tazara. Ana iya ba da ƙimar ƙididdiga don wasu samfuran sarrafa sera na kasuwanci ta MADx akan buƙata.

NAZARIN DATA

Don nazarin hoton da aka sarrafa, za a yi amfani da ImageXplorer ko na'urar MAX. Hotunan ALEX² ana nazarin su ta atomatik ta amfani da RAPTOR SERVER Analysis Software kuma ana samar da rahoto mai taƙaita sakamakon ga mai amfani.

SAKAMAKO
ALEX² gwajin ELISA ne mai ƙididdigewa don ƙayyadaddun IgE da hanyar ƙididdige ƙididdiga don jimlar IgE. Ana bayyana takamaiman ƙwayoyin rigakafin IgE a matsayin rukunin amsa IgE (kUA/l), jimlar sakamakon IgE azaman kU/l. RAPTOR SERVER Analysis Software yana ƙididdigewa ta atomatik kuma yana ba da rahoton sakamakon sIgE (ƙididdigewa) da sakamakon tIgE (bimi- ƙima).

IYAKA NA HANYA

Ya kamata a yi tabbataccen ganewar asali na asibiti kawai tare da duk abubuwan da aka samu na asibiti daga kwararrun likitocin kuma ba za a dogara da sakamakon hanyar ganowa ɗaya kawai ba.
A wasu wuraren aikace-aikacen (misali rashin lafiyar abinci), ƙwayoyin rigakafi na IgE masu yaduwa na iya zama wanda ba a iya gano su ba duk da cewa bayyanar rashin lafiyar abinci na iya kasancewa a cikin wani nau'in allergen, saboda waɗannan ƙwayoyin rigakafi na iya zama na musamman ga allergens waɗanda aka canza yayin sarrafa masana'antu, dafa abinci ko narkewa. don haka babu shi akan ainihin abincin da aka gwada majiyyaci da shi.
Sakamakon dafin mara kyau kawai yana nuna matakan da ba a iya ganowa na takamaiman ƙwayoyin rigakafi na IgE (misali saboda rashin fallasa na dogon lokaci) kuma baya hana wanzuwar hauhawar jini na asibiti ga ƙwari.
A cikin yara, musamman har zuwa shekaru 2, matsakaicin adadin tIgE ya yi ƙasa da na matasa da manya [7]. Don haka, ana tsammanin cewa a cikin mafi girman adadin yara waɗanda ba su wuce shekaru 2 jimlar matakin IgE ya ta'allaka ne a ƙasa da ƙayyadadden ƙayyadadden ƙayyadaddun ganowa ba. Wannan iyakance ba zai shafi takamaiman ma'aunin IgE ba.

DABI'U DA AKE TSIRA
Ƙungiya ta kusa tsakanin matakan rigakafin IgE-takamaiman alerji da cutar rashin lafiyar sananne ne kuma an kwatanta su sosai a cikin wallafe-wallafe [1]. Kowane majinyaci mai hankali zai nuna mutum IgE profile lokacin da aka gwada da ALEX². Amsar IgE tare da samples daga masu lafiya waɗanda ba su da rashin lafiyar za su kasance ƙasa da 0.3 kUA/l don allergens guda ɗaya da kuma tsantsar alerji lokacin da aka gwada su tare da ALEX². Yankin tunani don jimlar IgE a cikin manya shine <100 kU/l. Kyakkyawan aikin dakin gwaje-gwaje yana ba da shawarar cewa kowane dakin gwaje-gwaje ya kafa nasa kewayon ƙimar sa.

HALAYEN YI
Ana iya samun halayen aikin da kuma Takaitaccen Tsaro da Aiki akan MADx website: https://www.madx.com/extras.

GARANTI

An samo bayanan aikin ta amfani da hanyar da aka tsara a cikin wannan Umarnin don Amfani. Duk wani canji ko gyare-gyare a cikin hanyar na iya rinjayar sakamakon kuma MacroArray Diagnostics yana watsi da duk garantin da aka bayyana (gami da garantin ciniki da dacewa don amfani) a irin wannan taron. Saboda haka, MacroArray Diagnostics da masu rarrabawa na gida ba za su ɗauki alhakin lalacewa kaikaice ko mai ma'ana ba a irin wannan lamarin.

GASKIYA

ALEX	Allergy Xplorer
CCD	Ƙayyadaddun ƙwayoyin carbohydrate masu saurin amsawa
EDTA	Ethylenediaminetetraacetic acid
ELISA	Enzyme-Linked Immunosorbent Assay
IgE	Immunoglobulin E
IVD	In-vitro bincike
ku/l	Kilo raka'a a kowace lita
ku/l	Kilo raka'a na takamaiman IgE-allergen kowace lita
MADx	MacroArray Diagnostics
REF	Lambar magana
rpm	Zagaye a minti daya
sIgE	Allergen-takamaiman IgE
tIgE	Jimlar IgE
µl	Microliter

LISSAFI ALERGEN ALEX²

Allergen ruwan 'ya'yan itace: Aca m, Aca s, Ach d, Ail a, All c, All s, Ama r, Amb a, Ana o, Api m, Art v, Ave s, Ber e, Bos d nama, Bos d madara, Bro p , Cam d, Can f ♂ fitsari, Can s, Cap a, Cap h epithelia, Cap h madara, Car c, Mota i, Car p, Che a, Che q, Chi spp., Cic a, Cit s, Cla h , Clu h, Cor a pollen, Cuc p, Cup s, Cyn d, Dau c, Dol spp., Equ c madara, Equ c nama, Fag e, Fic b, Fic c, Fra e, Gad m, Gal d nama , Gal d fari, Gal d gwaiduwa, Hel a, Hom g, Hor v, Jug r, Jun a, Len c, Lit s, Loc m, Lol spp., Lup a, Mac i, Man i, Mel g, Mor r, Mus a, Myt e, Ori v, Ory nama, Ory s, Ost e, Ovi a epithelia, Ovi nama, Ovi a madara, Pan b, Pan m, Pap s, Par j, Pas n, Pec spp. , Pen ch, Per a, Pers a, Pet c, Pha v, Phr c, Pim a, Pis s, Pla l, Pol d, Pop n, Pru av, Pru du, Pyr c, Raj c, Rat n, Rud spp., Sac c, Sal k, Sal s, Sco s, Sec c gari, Sec c pollen, Ses i, Sin, Sol spp., Sola l, Sol t, Sus d epithel, Sus d nama, Goma m, Thu a, Tri fo, Tri s, Tyr p, Ulm c, Urt d, Vac m, Ves v, Zea m gari

Abubuwan da aka tsarkake na halitta: nAct d 1, nApi m 1, nAra h 1, nAra h 3, nBos d 4, nBos d 5, nBos d 6, nBos d 8, nCan f 3, nCor a 9, nCor a 11, nCup a 1, nCry j. 1, nEqu c 3, nFag e 2, nGad m 1, nGad m 2 + 3, nGal d 2, nGal d 3, nGal d 4, nGal d 5, nGly m 5, nGly m 6, nJug r 4, nMac i. 2S Albumin, nOle e 7 (RUO), nPap s 2S Albumin, nPis v 3, nPla a 2, nTri da aA_TI

Abubuwan da aka sake haɗawa: rAct d 10, rAct d 2, rAct d 5, rAln g 1, rAln g 4, rAlt a 1, rAlt a 6, rAmb a 1, rAmb a 4, rAna o 2, rAna o 3, rAnir s 1. 3, rApi g 1, rApi g 2, rApi g 6, rApi m 10, rAra h 2, rAra h 6, rAra h 8, rAra h 9, rAra h 15, rArg r 1, rArt v 1, 3 r rAsp f 1, rAsp f 3, rAsp f 4, rAsp f 6, rBer e 1, rBet v 1, rBet v 2, rBet v 6, rBla g 1, rBla g 2, rBla g 4, rBla g 5, 9, rBlo t 10, rBlo t 21, rBlo t 5, rBos d 2, rCan f 1, rCan f 2, rCan f 4, rCan f 6, rCan f 1 kama, rCan s 3, rCarp p 1 a 1, rCla h 8, rClu h 1, rClu h 1.0103, rCor a 1.0401, rCor a 8, rCor a 12, rCor a 14 (RUO), rCor a 6, rCra c 2, , rCuc m 1, cr 1p , rDau c 1, rDer f 1, rDer f 2, rDer p 1, rDer p 10, rDer p 11, rDer p 2, rDer p 20, rDer p 21, rDer p 23, rDer p 5, rDer p 7, rDer p 1, 4 c 1, rEqu c 1, rFag s 2, rFel d 4, rFel d 7, rFel d 1, rFel d 3, rFra a 1 + 1, rFra e 2, rGal d 4, rGly d 8, rGly m 1, m 3, rHev b 5, rHev b 6.02, rHev b 8, rHev b 11, rHev b 1, rHev b 2, rHom s LF, rJug r 3, rJug r 6, rJug r 2, 1, 1 Jurg , rLol p 3, rMal d 11, rMal d 5, rMala s 6, rMala s 2, rMala s 1, rMal d 1, rMer a 1, rMes a 1 (RUO), rMus m 9, rOle e 1, 2, rOry c 3, rOry c 2, rOry c 1, rPar j 2, rPen m 3, rPen m 4, rPen m 7, rPen m 1, rPer a 12, rPhl p 2, rPhl p 5.0101, rPhl . rPhl p 6, rPhl p 7, rPhl p 2, rPho d 1, rPhod s 1, rPis v 2, rPis v 4, rPis v 1 (RUO), rPla a 3, rPla a 1, rPla l 5, , rPru p 3, rPru p 7 (RUO), rRaj c Parvalbumin, rSal k 1, rSal s 1, rSco s 1, rSes i 1, rSin a 1, rSola l 6, rSus d 1, rThu a 1, 14, rTri a 19, rTyr p 2, rVes v 1, rVes v 5, rVit v 1, rXip g 1, rZea m 14

NASARA

Hamilton, RG. (2008). Kimanta cututtukan rashin lafiyar ɗan adam. Clinical Immunology. 1471-1484. 10.1016/B978-0-323-04404-2.10100-9.
Harwanegg C, Laffer S, Hiller R, Mueller MW, Kraft D. Clin Exp Allergy. 2003 Jan; 33 (1): 7-13. doi: 10.1046/j.1365-2222.2003.01550.x. Saukewa: 12534543.

Hiller R, Laffer S, Harwanegg C, Huber M, Schmidt WM, Twardosz A, Barletta B, Becker WM, Blaser K, Breiteneder H, Chapman M. King TP, Kleber-Janke T, Kurup VP, Lehrer SB, Lidholm J, Müller U, Pini C, Reese G, Scheiner O, Scheynius A, Shen HD, Spitzauer S, Suck R. Van Hage-Hamsten M, Virtanen T, Kraft D, Müller MW, Valenta R. Kwayoyin alerji masu ƙima: masu gadin ƙofofi don maganin alerji. FASEB J. 2002 Mar; 16 (3): 414-6. doi: 10.1096/fj.01-0711fje. Epub 2002 Jan 14. PMID: 11790727
Ferrer M, Sanz ML, Sastre J, Bartra J, del Cuvillo A, Montoro J, Jáuregui I, Dávila I, Mullol J, Valero A. Kwayoyin cututtuka a cikin allergology: aikace-aikacen fasaha na microarray. J Bincika Allergol Clin Immunol. 2009;19 Shafi 1:19-24. Saukewa: 19476050.
Ott H, Fölster-Holst R, Merk HF, Baron JM. Allergen microarrays: wani labari kayan aiki don high-ƙuduri IgE profileing a manya da atopic dermatitis. Farashin J Dermatol. 2010 Jan-Fabrairu;20(1):54-
61. doi: 10.1684/ejd.2010.0810. Epub 2009 Oct 2. PMID: 19801343.

Sastre J. Kwayoyin cututtuka a cikin rashin lafiyan. Clin Exp Allergy. 2010 Oktoba; 40 (10): 1442-60. doi: 10.1111/j.1365-2222.2010.03585.x. Epub 2010 Agusta 2. PMID: 20682003.
Martins TB, Bandhauer ME, Bunker AM, Roberts WL, Hill HR. Sabbin tazara tsakanin yara da manya don jimlar IgE. J Allergy Clin Immunol. 2014 Fabrairu; 133 (2): 589-91.

Don cikakkun bayanai kan binciken bincike da na asibiti da aka yi duba ga halayen aiki a https://www.madx.com/extras.

CANJIN TARIHI

Sigar	Bayani	Maye gurbin
11	nGal d1 ya canza zuwa rGal d1; URL sabunta zuwa madx.com; CE kari tare da lambar Jikin Sanarwa; canza tarihi kara	10

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