MacroArray ALLERGY XPLORER Macro Array Diagnostics
Litlhaloso
- Lebitso la Sehlahisoa: Basic UDI-DI 91201229202JQ
- Linomoro tsa Litšupiso: REF 02-2001-01, 02-5001-01
- Tšebeliso e Reriloeng: Ho fumanoa ha allergen-specific IgE (sIgE) ka bongata le kakaretso ea IgE (tIgE) semi-quantitatively
- Basebelisi: Basebetsi ba koetlisitsoeng ba laboratori le litsebi tsa bongaka ka laboratoring ea bongaka
- Polokelo: Lisebelisoa tsa Kit li tsitsitse likhoeli tse 6 kamora ho buloa
Litaelo tsa Tšebeliso ea Sehlahisoa
Molao-motheo oa Tsamaiso
Sehlahisoa se lemoha allergen-specific IgE ka bongata le kakaretso ea IgE semi-quantitatively.
Thomello le Polokelo
Netefatsa hore lisebelisoa tsa lisebelisoa li bolokiloe joalo ka ha ho boletsoe 'me li sebelisoa nakong ea likhoeli tse 6 ho tloha ha ho buloa.
Ho Lahla Litšila:
Latela mekhoa e nepahetseng ea ho lahla litšila ho latela melaoana.
Likarolo tsa Kit
Sheba bukana ea mosebelisi bakeng sa tlhaiso-leseling e batsi ka likarolo tsa kit.
Thepa e Hlokehang
Tlhahlobo ea Matsoho: Etsa bonnete ba hore u na le lisebelisoa tse hlokahalang tse fanoeng ke moetsi.
Tlhahlobo e Ikemetseng: Sebelisa sesebelisoa sa MAX, Tharollo ea ho Hlatsoa, Stop Solution, RAPTOR SERVER Analysis Software, le PC/Laptop. Latela litaelo tsa tlhokomelo ka hloko.
Ho sebetsana le Arrays
Latela litaelo tse fanoeng bakeng sa ho sebetsana le lihlopha ka hloko ho netefatsa liphetho tse nepahetseng.
Litemoso le Litlhokomeliso
- Apara lisebelisoa tse nepahetseng tsa ts'ireletso joalo ka ts'ireletso ea letsoho le mahlo le lijase tsa lab.
- Tšoara li-reagents le sampho latela mekhoa e metle ea laboratori.
- Tšoara lisebelisoa tsohle tsa batho e le tse ka tšoaetsanoang 'me u li tšoare ka hloko.
LBH
- P: Li-reagents tsa kit li tsitsitse nako e kae?
A: Li-reagents tsa Kit li tsitsitse likhoeli tsa 6 ka mor'a ho buloa ha li bolokiloe tlas'a maemo a bontšitsoeng. - P: Ke mang ea ka sebelisang sehlahisoa see?
K: Sehlahisoa sena se reretsoe ho sebelisoa ke basebetsi ba koetlisitsoeng ba laboratori le litsebi tsa bongaka maemong a laboratori ea bongaka.
www.madx.com
ALLERGY XPLORER (ALEX²) TAELO HO SEBELISA
TLHALOSO
Allergy Xplorer (ALEX²) ke Enzyme-Linked Immunosorbent Assay (ELISA) - e thehiloeng litekong tsa tlhahlobo ea invitro bakeng sa tekanyo ea palo ea allergen-specific IgE (sIgE).
Taelo ena ea Tšebeliso e sebetsa ho lihlahisoa tse latelang:
| UDI-DI ea motheo | REF | Sehlahisoa |
| 91201229202JQ | 02-2001-01 | ALEX² bakeng sa Litlhahlobo tse 20 |
| 02-5001-01 | ALEX² bakeng sa Litlhahlobo tse 50 |
THABELO MAEMO
ALEX² Allergy Xplorer ke sesebelisoa sa liteko se sebelisetsoang tlhahlobo ea invitro ea serum ea motho kapa plasma (ntle le EDTA-plasma) ho fana ka leseli ho thusa ho fumana bakuli ba nang le mafu a IgE hammoho le liphetho tse ling tsa bongaka kapa liphetho tsa tlhahlobo ea mafu. .
Sesebelisoa sa bongaka sa IVD se lemoha allergen-specific IgE (sIgE) ka bongata le kakaretso ea IgE (tIgE) semi-quantitatively. Sehlahisoa se sebelisoa ke basebetsi ba koetlisitsoeng ba laboratori le litsebi tsa bongaka ka laboratoring ea bongaka.
KAKARETSO LE TLHALOSO EA TEKO
Matšoao a ho kula ke mofuta oa hang-hang oa hypersensitivity ho mofuta oa I 'me o kena lipakeng ke li-antibodies tsa sehlopha sa IgE sa immunoglobulins. Ka mor'a ho pepesehela lintho tse ling tse itseng, ho lokolloa ha histamine le bakena-lipakeng ba bang ho tloha liseleng tsa mast le basophils ho fella ka matšoao a kang asthma, allergic rhino-conjunctivitis, atopic eczema le matšoao a mala. Ka hona, mokhoa o hlakileng oa ts'usumetso ho li-allergen tse itseng o thusa ho lekola bakuli ba allergic [1-2]. Ha ho na thibelo ho palo ea batho ba etsang liteko. Ha ho etsoa liteko tsa IgE, lilemo le thobalano hangata ha li nkoe e le lintlha tsa bohlokoa hobane maemo a IgE, a lekantsoeng litlhahlobong tsena, ha a fapane haholo ho latela palo ea batho.
Mehloli eohle e meholo ea mofuta oa I e koahetsoe ke ALEX². Lenane le felletseng la li-extracts tsa ALEX² tsa allergen le limolek'hule li ka fumanoa botlaaseng ba taelo ena.
Lintlha tsa bohlokoa bakeng sa mosebelisi!
Bakeng sa ts'ebeliso e nepahetseng ea ALEX², hoa hlokahala hore mosebelisi a bale ka hloko le ho latela litaelo tsa tšebeliso. Moetsi ha a nke boikarabello bakeng sa ts'ebeliso efe kapa efe ea sistimi ena ea liteko e sa hlalosoang tokomaneng ena kapa bakeng sa liphetoho ke mosebelisi oa sistimi ea liteko.
Tlhokomeliso: Phapang ea kit 02-2001-01 ea teko ea ALEX² (20 Arrays) e etselitsoe ho sebetsa ka matsoho feela. Ho sebelisa mofuta ona oa ALEX² o nang le automated MAX 9k, Washing Solution (REF 00-5003-01) le Stop Solution (REF 00-5007-01) li hloka ho odara ka thoko. Lintlha tsohle tse ling tsa sehlahisoa li ka fumanoa litaelong tse lumellanang tsa tšebeliso: https://www.madx.com/extras.
Phapang ea ALEX² kit 02-5001-01 (50 arrays) e ka sebelisoa bakeng sa ts'ebetso ea othomathiki ka MAX 9k (REF 17-0000-01) hammoho le sesebelisoa sa MAX 45k (REF 16-0000-01).
MOLAO OA MOKHOA
ALEX² ke tlhahlobo ea immunoassay e thehiloeng ho Enzyme-Linked Immunosorbent Assay (ELISA). Li-extracts tsa Allergen kapa allergens ea molek'hule, tse kopantsoeng le nanoparticles, li behiloe ka mokhoa o hlophisehileng karolong e tiileng ea ho etsa sehlopha sa macroscopic. Taba ea pele, li-aleji tse nang le likaroloana li itšoara ka IgE e itseng e teng 'meleng oa mokuliample. Ka mor'a ho qhoqhoa, IgE e seng e tobileng ea hlatsuoa. Ts'ebetso e tsoela pele ka ho kenyelletsa anti-human IgE antibody e ngotsoeng ka enzyme e etsang motsoako o nang le likaroloana tsa IgE tse khethehileng. Ka mor'a mohato oa bobeli oa ho hlatsoa, substrate e kenyelletsoa e fetoloang ho sa qhibilihe, se nang le mebala e mengata ka enzyme e kopantsoeng le antibody. Qetellong, karabelo ea enzyme-substrate e emisoa ka ho eketsa reagent e thibelang. Palo ea pula e lekana le khatello ea IgE e itseng 'meleng oa mokuliample. Ts'ebetso ea tlhahlobo ea lab e lateloa ke ho fumana litšoantšo le tlhahlobo ho sebelisoa sistimi ea matsoho (ImageXplorer) kapa sistimi e ikemetseng (MAX 45k kapa MAX 9k). Liphetho tsa liteko li sekasekoa ka RAPTOR SERVER Analysis Software 'me li tlaleheloa likarolong tsa karabelo tsa IgE (kUA/l). Kakaretso ea liphetho tsa IgE le tsona li tlalehoa ho likarolo tsa karabelo tsa IgE (kU/l). RAPTOR SERVER e fumaneha ka mofuta oa 1, bakeng sa nomoro ea mofuta oa linomoro tse 'ne ka kopo sheba mongolo oa RAPTOR SERVER o fumanehang ho. www.raptor-server.com/imprint.
ROMANE LE POLOKELO
Ho romelloa ha ALEX² ho etsahala maemong a mocheso a tikoloho. Leha ho le joalo, setlolo se tlameha ho bolokoa hang ha se beleha ka mocheso oa 2-8 ° C. E bolokiloe ka nepo, ALEX² le likarolo tsa eona li ka sebelisoa ho fihlela ka letsatsi le bontšitsoeng la ho felloa ke nako.
Li-reagents tsa Kit li tsitsitse likhoeli tse 6 ka mor'a ho buloa (ka maemo a bontšitsoeng a polokelo).
TLHOKOMELO MAHALA
Lahla khatriji e sebelisitsoeng ea ALEX² le lisebelisoa tse sa sebelisoeng tse nang le litšila tsa lik'hemik'hale tsa laboratoring. Latela melao eohle ea naha, naha, le ea lehae mabapi le ho lahloa.
LIEKETSENG LITŠOANTŠO
LIEKETSENG KOTLO
Karolo ka 'ngoe (reagent) e tsitsitse ho fihlela letsatsi le boletsoeng leiboleng ea karolo ka 'ngoe. Ha ho khothalletsoe ho kopanya li-reagents tse tsoang lotho e fapaneng ea kit. Bakeng sa lethathamo la lintho tse qotsitsoeng le limolek'hule tse sa sebetseng ka har'a sehlopha sa ALEX², ka kopo ikopanye. support@madx.com.
| Likaroloana tsa Kit REF 02-2001-01 | Litaba | Thepa |
| ALEX² Cartridge | Li-blister tse 2 ho 10 ALEX² bakeng sa litlhahlobo tse 20 ka kakaretso.
Calibration ka master curve e fumaneha ka RAPTOR SERVER Analysis Software. |
E loketse ho sebelisoa. Boloka ho 2-8 ° C ho fihlela letsatsi la ho felloa ke nako. |
| ALEX² Sample Diluent | 1 botlolo ho 9 ml | E loketse ho sebelisoa. Boloka ho 2-8 ° C ho fihlela letsatsi la ho felloa ke nako. Lumella reagent ho fihlela mocheso oa kamore pele o sebelisoa. Reagent e butsoeng e tsitsitse likhoeli tse 6 ho 2-8 ° C, e kenyelletsa CCD inhibitor. |
| Ho hlatsoa Tharollo | 2 botlolo ho 50 ml | E loketse ho sebelisoa. Boloka ho 2-8 ° C ho fihlela letsatsi la ho felloa ke nako. Lumella reagent ho fihlela mocheso oa kamore pele o sebelisoa. Reagent e butsoeng e tsitsitse likhoeli tse 6 ho 2-8 ° C. |
| Likaroloana tsa Kit REF 02-2001-01 | Litaba | Thepa |
| ALEX² Sethibela-Motho sa ho Khetholla | 1 botlolo ho 11 ml | E loketse ho sebelisoa. Boloka ho 2-8 ° C ho fihlela letsatsi la ho felloa ke nako. Lumella reagent ho fihlela mocheso oa kamore pele o sebelisoa. Reagent e butsoeng e tsitsitse likhoeli tse 6 ho 2-8 ° C. |
| ALEX² Substrate Tharollo | 1 botlolo ho 11 ml | E loketse ho sebelisoa. Boloka ho 2-8 ° C ho fihlela letsatsi la ho felloa ke nako. Lumella reagent ho fihlela mocheso oa kamore pele o sebelisoa. Reagent e butsoeng e tsitsitse likhoeli tse 6 ho 2-8 ° C. |
| (ALEX²) Emisa Tharollo | 1 botlolo ho 2.4 ml | E loketse ho sebelisoa. Boloka ho 2-8 ° C ho fihlela letsatsi la ho felloa ke nako. Lumella reagent ho fihlela mocheso oa kamore pele o sebelisoa. Reagent e butsoeng e tsitsitse likhoeli tse 6 ho 2-8 ° C. E ka hlaha e le tharollo ea moferefere ka mor'a polokelo ea nako e telele. Sena ha se na phello ho liphetho. |
| Likaroloana tsa Kit REF 02-5001-01 | Litaba | Thepa |
| ALEX² Cartridge | Li-blister tse 5 ho 10 ALEX² bakeng sa litlhahlobo tse 50 ka kakaretso.
Calibration ka master curve e fumanehang ka RAPTOR SERVER Analysis Software. |
E loketse ho sebelisoa. Boloka ho 2-8 ° C ho fihlela letsatsi la ho felloa ke nako. |
| ALEX² Sample Diluent | 1 botlolo ho 30 ml | E loketse ho sebelisoa. Boloka ho 2-8 ° C ho fihlela letsatsi la ho felloa ke nako. Lumella reagent ho fihlela mocheso oa kamore pele o sebelisoa. Reagent e butsoeng e tsitsitse likhoeli tse 6 ho 2-8 ° C, e kenyelletsa CCD inhibitor. |
| Ho hlatsoa Tharollo | 4 x leqe. 1 botlolo ho 250 ml | Boloka ho 2-8 ° C ho fihlela letsatsi la ho felloa ke nako. Hlakola 1 ho 4 ka metsi a demineralized pele o sebelisoa. Lumella reagent ho fihlela mocheso oa kamore pele o sebelisoa. Reagent e butsoeng e tsitsitse likhoeli tse 6 ho 2-8 ° C. |
| ALEX² Sethibela-Motho sa ho Khetholla | 1 botlolo ho 30 ml | E loketse ho sebelisoa. Boloka ho 2-8 ° C ho fihlela letsatsi la ho felloa ke nako. Lumella reagent ho fihlela mocheso oa kamore pele o sebelisoa. Reagent e butsoeng e tsitsitse likhoeli tse 6 ho 2-8 ° C. |
| Likaroloana tsa Kit REF 02-5001-01 | Litaba | Thepa |
| ALEX² Substrate Tharollo | 1 botlolo ho 30 ml | E loketse ho sebelisoa. Boloka ho 2-8 ° C ho fihlela letsatsi la ho felloa ke nako. Lumella reagent ho fihlela mocheso oa kamore pele o sebelisoa. Bule reagent ke
e tsitsitseng bakeng sa likhoeli tse 6 ho 2-8°C. |
| (ALEX²) Emisa Tharollo | 1 botlolo ho 10 ml | E loketse ho sebelisoa. Boloka ho 2-8 ° C ho fihlela letsatsi la ho felloa ke nako. Lumella reagent ho fihlela mocheso oa kamore pele o sebelisoa. Reagent e butsoeng e tsitsitse likhoeli tse 6 ho 2-8 ° C. E ka hlaha e le tharollo ea moferefere ka mor'a polokelo ea nako e telele. Sena ha se na phello ho liphetho. |
SEHLOPHA SE HLOKANG HO SEBELISA LE HO HLAHLOBA
Tlhahlobo ea Bukana
- ImageXplorer
- Arrayholder (ho ikhethela)
- Lab Rocker (angle ea tšekamelo 8°, lebelo le hlokahalang 8 rpm)
- Kamore ea ho holisa (WxDxH - 35x25x2 cm)
- RAPTOR SERVER Analysis Software
- PC/Laptop
Lisebelisoa tse hlokahalang, tse sa fanoeng ke MADx:
- Metsi a Demineralized
- Liphaephe le malebela (100 µl & 100 - 1000 µl)
Tlhahlobo e Ikemetseng:
- Sesebelisoa sa MAX (MAX 45k kapa MAX 9k)
- Tharollo ea ho Hlatsoa (REF 00-5003-01)
- Emisa Tharollo (REF 00-5007-01)
- RAPTOR SERVER Analysis Software
- PC/Laptop
Litšebeletso tsa tlhokomelo ho latela litaelo tsa moetsi.
TS'OOA ARRAY
Se ke oa ama bokaholimo ba sehlopha. Litšitiso leha e le life tse ka holim'a metsi tse bakoang ke lintho tse hlabang kapa tse bohale li ka kena-kenana le ho bala hantle ha liphetho. Se ke oa fumana litšoantšo tsa ALEX² pele sehlopha se omella ka botlalo (o omme mocheso oa kamore).
TLHOKOMELISO LE TS'ELISO
- Ho kgothaletswa ho apara tshireletso ea matsoho le mahlo hammoho le lijase tsa lab le ho latela mekhoa e metle ea laboratori ha u lokisetsa le ho sebetsana le li-reagents le s.amples.
- Ho latela ts'ebetso e ntle ea laboratori, lisebelisoa tsohle tsa motho li lokela ho nkuoa li ka tšoaetsanoa 'me li sebetsoe ka litemoso tse tšoanang le tsa mokuli.amples.
- ALEX² Sample Diluent and Washing Solution e na le azide ea sodium (<0.1%) e le ntho e sireletsang 'me e tlameha ho sebetsoa ka hloko. Letlapa la lintlha tsa polokeho le fumaneha ha le kopa.
- The (ALEX²) Stop Solution e na le Ethylenediaminetetraacetic acid (EDTA) -Tharollo mme e tlameha ho sebetsoa ka hloko. Letlapa la lintlha tsa polokeho le fumaneha ha le kopa.
- Bakeng sa ts'ebeliso ea tlhahlobo ea invitro feela. Eseng bakeng sa tshebediso ya ka hare kapa kantle ho batho kapa diphoofolo.
- Ke basebetsi ba koetliselitsoeng tšebetsong ea laboratori feela ba lokelang ho sebelisa sephutheloana sena.
- Ha u fihla, hlahloba likarolo tsa kit bakeng sa tšenyo. Haeba e 'ngoe ea likarolo e senyehile (mohlala, libotlolo tsa buffer), ikopanye le MADx (support@madx.com) kapa morekisi oa lehae oa hau. U se ke ua sebelisa lisebelisoa tsa kit tse senyehileng, kaha tšebeliso ea tsona e ka lebisa ho se sebetse hantle ha lisebelisoa.
- Se ke oa sebelisa li-reagents ho feta matsatsi a tsona a ho felloa ke nako.
- Se ke oa kopanya li-reagents tse tsoang lihlopheng tse fapaneng.
ELISA MOKHOA
Tokisetso
Tokisetso ea samples: Serum kapa plasma (heparin, citrate, ha ho EDTA) sampho ka sebelisoa mali a capillary kapa venous. Mali sampli ka bokelloa ho sebelisoa mekhoa e tloaelehileng. Lebenkele sampka tlase ho 2-8 ° C ho fihlela beke e le 'ngoe. Boloka serum le plasmaampka tlase ho -20°C bakeng sa ho boloka nako e telele. Ho romelloa ha serum/plasma sampmocheso o fokolang o sebetsa. Lumella kamehla sampho fihlela mocheso oa kamore pele o sebelisoa.
Tokisetso ea Tharollo ea ho Hlatsoa (feela bakeng sa REF 02-5001-01 le REF 00-5003-01 ha e sebelisoa le sesebelisoa sa MAX): Tšela litaba tsa 1 vial ea Washing Solution ka har'a setshelo sa ho hlatsoa sa sesebelisoa. Tlatsa metsi a demineralized ho fihlela letšoao le khubelu 'me u kopanye ka hloko setshelo ka makhetlo a mangata ntle le ho hlahisa foam. Reagent e butsoeng e tsitsitse likhoeli tse 6 ho 2-8 ° C.
Incubation chamber: Koala sekoahelo bakeng sa mehato eohle ea tlhahlobo ho thibela ho theoha ha mongobo.
Mekhahlelo of Mokhoa:
- 100µl sample + 400 µl ALEX² Sample Diluent
- 500 µl ALEX² Sethibela-mafu sa ho Khetholla
- 500 µl ALEX² Substrate Tharollo
- 100 µl (ALEX²) Emisa Tharollo
- 4500 µl Tharollo ea ho Hlatsoa
Nako ea tlhahlobo ke hoo e ka bang 3 h 30 min (ntle le ho omisoa ha mefuta e hlophisitsoeng).
Ha ho kgothaletswe ho etsa liteko tse ngata ho feta kamoo ho ka kenngoa liphaephe ka 8 min. Li-incubations tsohle li etsoa ka mocheso oa kamore, 20-26 ° C.
Li-reagents tsohle li lokela ho sebelisoa mocheso oa kamore (20-26 ° C). Teko ha ea lokela ho etsoa ka khanya ea letsatsi.
Lokisetsa kamore ea ho qhoqhoa
Bula kamore ea ho incubation 'me u behe lithaole tsa pampiri karolong e ka tlaase. Soak lithaole tsa pampiri ka metsi a demineralized ho fihlela ho se na likarolo tse omeletseng tsa lithaole tsa pampiri tse bonahalang.
Sample incubation/CCD inhibition
Ntša palo e hlokahalang ea lik'hatriji tsa ALEX² 'me u li behe ka har'a li-array holder. Kenya 400 μl ea ALEX² Sample Diluent ho khatriji ka 'ngoe. Kenya 100 μl mokuli sample ho li-cartridges. Etsa bonnete ba hore tharollo e hlahisoang e hasana ka tsela e tšoanang. Beha li-cartridges ka kamoreng e lokiselitsoeng ea ho qhoqhoa 'me u kenye kamore ea ho qhoqhoa ka li-cartridges holim'a lab rocker e le hore li-cartridges li reke ka lehlakoreng le lelelele la cartridge. Qala serum incubation ka 8 rpm bakeng sa lihora tse 2. Koala kamore ea ho incubation pele u qala lab rocker. Ka mor'a lihora tse 2, tlosa sampka setshelong sa pokello. Hlakola ka hloko marotholi a ka har'a khatriji u sebelisa thaole ea pampiri.
Qoba ho ama bokaholimo ba marang-rang ka thaole ea pampiri! Qoba leha e le efe ho jara kapa ho tšela tšilafalo ea samphanyane lipakeng tsa li-cartridge tsa ALEX²!
Taba ea boikhethelo kapa e ntle ea Hom s LF (CCD lesupa): ka protocol e tloaelehileng ea CCD antibody (joalokaha ho hlalositsoe serapeng sa 2: sample incubation/CCD inhibition) ts'ebetso ea ho thibela CCD ke 85%. Haeba tekanyo e phahameng ea ho thibela katleho e hlokahala, lokisetsa 1 ml sample tube, eketsa 400 μl ALEX² Sample Diluent le 100 μl serum. Eketsa metsotso e 30 (e sa sisinyeheng) ebe o tsoela pele ka mokhoa o tloaelehileng oa tlhahlobo.
Hlokomela: Mohato o eketsehileng oa ho thibela CCD o lebisa maemong a mangata sekhahla sa ho thibela li-antibodies tsa CCD tse ka holimo ho 95%.
1a. Ho hlatsoa I
Kenya 500 μl Washing Solution ho khatriji e 'ngoe le e 'ngoe 'me u e kenye holim'a rocker ea lab (ka 8 rpm) metsotso e mehlano. Lahlela Tharollo ea ho Hlatsoa ka har'a sets'oants'o sa pokello 'me u tobetse ka matla li-cartridges holim'a qubu ea lithaole tsa pampiri tse omeletseng. Hlakola ka hloko marotholi a setseng ho li-cartridges u sebelisa thaole ea pampiri.
Pheta mohato ona makhetlo a 2 hape.
Kenya sethibela-mahlahahlaha
Eketsa 500 µl ea ALEX² Sethibela-Motho sa Tlhahlobo ho khatriji ka 'ngoe.
Etsa bonnete ba hore sebaka se felletseng se koahetsoe ke tharollo ea ALEX² Detection Antibody.
Beha li-cartridges ka kamoreng ea ho qhoqhoa ka har'a "lab rocker" 'me u e kenye ka 8 rpm metsotso e 30. Lahlela tharollo ea Detection Antibody ka har'a sets'oants'o sa pokello 'me u tobetse ka matla likhatriji holim'a qubu ea lithaole tsa pampiri tse omeletseng. Hlakola ka hloko marotholi a setseng ho li-cartridges u sebelisa thaole ea pampiri.
2a. Ho hlatsoa II
Kenya Tharollo ea ho Hlatsoa ea 500 μl khatrijing e 'ngoe le e 'ngoe 'me u e kenye ka har'a rocker ea lab ka 8 rpm metsotso e 5. Lahlela Tharollo ea ho Hlatsoa ka har'a sets'oants'o sa pokello 'me u tobetse ka matla li-cartridges holim'a qubu ea lithaole tsa pampiri tse omeletseng. Hlakola ka hloko marotholi a setseng ho li-cartridges u sebelisa thaole ea pampiri.
Pheta mohato ona makhetlo a 4 hape.
3+4. Eketsa ALEX² Substrate Solution 'me u emise karabelo ea substrate
Kenya 500 μl ea ALEX² Substrate Solution ho khatriji ka 'ngoe. Qala sebali sa nako ka ho tlatsa khatriji ea pele 'me u tsoele pele ka ho tlatsa li-cartridge tse setseng. Etsa bonnete ba hore bokaholimo bo felletseng bo koahetsoe ke Tharollo ea Substrate 'me u kenye lihlopha ka metsotso e 8 hantle ntle le ho sisinyeha (lab rocker ka 0 rpm le sebakeng se otlolohileng).
Ka mor'a metsotso e 8 hantle, eketsa 100 μl ea (ALEX²) Stop Solution ho li-cartridges tsohle, ho qala ka cartridge ea pele ho netefatsa hore lihlopha tsohle li kenngoa ka nako e le 'ngoe le ALEX² Substrate Solution. Hlohlelletsa ka hloko ho tsamaisa (ALEX²) Stop Solution ka har'a li-cartridges tse ngata, ka mor'a hore (ALEX²) Stop Solution e kenngoe liphaepheng tsohle. Ka mor'a moo, ntša (ALEX²) Substrate/Stop Solution ho li-cartridges 'me u tobetse ka matla qubu ea lithaole tsa pampiri tse omeletseng. Hlakola ka hloko marotholi leha e le afe a setseng ho li-cartridges u sebelisa thaole ea pampiri.
Lab Rocker HA E TLAMEHA HO TSIKAMA nakong ea ho kenya substrate!
4a. Ho hlatsoa III
Kenya Tharollo ea ho Hlatsoa ea 500 μl khatrijing e 'ngoe le e 'ngoe 'me u e kenye holim'a rocker ea lab ka 8 rpm bakeng sa metsotsoana e 30. Lahlela Tharollo ea ho Hlatsoa ka har'a sets'oants'o sa pokello 'me u tobetse ka matla li-cartridges holim'a qubu ea lithaole tsa pampiri tse omeletseng. Hlakola ka hloko marotholi leha e le afe a setseng ho li-cartridges u sebelisa thaole ea pampiri.
Tlhahlobo ea litšoantšo
Ka mor'a ho qeta mokhoa oa ho hlahloba, omisa li-arrays ka mocheso oa kamore ho fihlela o omme ka ho feletseng (o ka nka metsotso e 45).
Ho omisa ka ho feletseng ho bohlokoa bakeng sa kutloisiso ea teko. Ke feela lihlopha tse omisitsoeng ka ho feletseng tse fanang ka pontšo e nepahetseng ho tekanyo ea lerata.
Qetellong, li-arrays tse omisitsoeng li hlahlojoa ka ImageXplorer kapa sesebelisoa sa MAX 'me li hlahlojoe ka software ea RAPTOR SERVER Analysis (sheba lintlha tse ho RAPTOR SERVER software handbook). Software ea RAPTOR SERVER Analysis Software e netefalitsoe feela hammoho le sesebelisoa sa ImageXplorer le lisebelisoa tsa MAX, kahoo MADx ha e nke boikarabelo leha e le bofe bakeng sa liphello, tse fumanoeng ka sesebelisoa leha e le sefe sa ho nka litšoantšo (joaloka li-scanner).
Tekanyetso ea liteko
ALEX² master calibration curve e thehiloe ka tlhahlobo ea litšupiso khahlano le litokisetso tsa serum tse nang le IgE e ikhethileng khahlano le li-antigen tse fapaneng tse koahelang sebaka se reriloeng sa ho lekanya. Litekanyetso tse ngata tse khethehileng tsa calibration li fanoa ke RAPTOR SERVER Analysis Software. Liphetho tsa liteko tsa ALEX² sIgE li hlahisoa joalo ka kUA/l. Kakaretso ea liphetho tsa IgE li baloa ho tloha tekanyong ea anti-IgE e nang le lintlha tse khethehileng tsa calibration, tse fanoang ke RAPTOR SERVER Analysis Software 'me li khethoa ho latela lotho e khethehileng ea QR-code.
Litekanyetso tsa Curve bakeng sa lotho e 'ngoe le e' ngoe li lokisoa ke sistimi ea tlhahlobo ea litšupiso tsa ka tlung, khahlano le litokisetso tsa serum tse lekiloeng ho ImmunoCAP (Thermo Fisher Scientific) bakeng sa IgE e khethehileng khahlano le lintho tse fapaneng tse fapaneng. Ka hona, liphetho tsa ALEX² li ka lateloa ka tsela e sa tobang khahlano le boitokisetso ba litšupiso tsa WHO 11/234 bakeng sa kakaretso ea IgE.
Liphapang tse hlophisehileng tsa maemo a mats'oao lipakeng tsa lotho li tloaeleha ka ho leka-lekana ha heterologous khahlano le curve ea referense ea IgE. Tekanyo ea tokiso e sebelisoa ho ikamahanya le maemo bakeng sa liphapang tsa litekanyo tse itseng.
Mekhahlelo ea ho lekanya
IgE e khethehileng: 0.3-50 kUA / l palo
Kakaretso ea IgE: 20-2500 kU/l semi-quantitative
MOLAOLI KHABANE
Ho boloka rekoto bakeng sa tlhahlobo ka 'ngoe
Ho latela ts'ebetso e ntle ea laboratori, ho khothaletsoa ho hatisa palo ea li-reagents tsohle tse sebelisitsoeng.
Laola Mehlala
Ho latela ts'ebetso e ntle ea laboratori ho khothaletsoa hore taolo ea boleng sampli kenyelelitsoe ka har'a linako tse hlalositsoeng. Lintlha tsa litšupiso bakeng sa sera se itseng sa taolo se fumanehang khoebong li ka fanoa ke MADx ha u e kopa.
TS'EBETSO TSEBA
Bakeng sa tlhahlobo ea litšoantšo tsa li-arrays tse hlophisitsoeng, ho tla sebelisoa ImageXplorer kapa sesebelisoa sa MAX. Litšoantšo tsa ALEX² li hlahlobjoa ka bo eona ho sebelisoa RAPTOR SERVER Analysis Software 'me ho hlahisoa tlaleho e akaretsang liphetho tsa mosebelisi.
LIPHETHO
ALEX² ke tlhahlobo ea ELISA ea tlhahlobo e khethehileng ea IgE le mokhoa oa semi-quantitative bakeng sa kakaretso ea IgE. Li-antibodies tsa IgE tsa Allergen li hlahisoa e le likarolo tsa karabelo tsa IgE (kUA/l), kakaretso ea liphetho tsa IgE joalo ka kU/l. RAPTOR SERVER Analysis Software e ipabola le ho tlaleha liphetho tsa sIgE (ka bongata) le liphetho tsa tIgE (semi- quantitatively).
LIEKETSENG MOKHOPO
Tlhahlobo e nepahetseng ea kliniki e lokela ho etsoa feela hammoho le liphuputso tsohle tse fumanehang tsa kliniki ke litsebi tsa bongaka 'me e ke ke ea itšetleha ka liphello tsa mokhoa o le mong oa tlhahlobo feela.
Likarolong tse ling tsa ts'ebeliso (mohlala, allergy ea lijo), li-antibodies tsa IgE tse potolohang li ka 'na tsa lula li sa lemohuoe, leha ho ka ba le ponahatso ea bongaka ea ho hana lijo khahlano le ntho e itseng, hobane lisireletsi tsena e kanna ea ba tse tobileng ho lintho tse allergy tse fetotsoeng nakong ea ts'ebetso ea indasteri, ho pheha kapa tšilo ea lijo. ka hona ha li be teng lijong tsa pele tseo mokuli a hlahlojoang ka tsona.
Liphetho tse mpe tsa chefo li bontša feela maemo a sa bonahaleng a li-antibodies tsa mahloko a IgE (mohlala, ka lebaka la ho se pepesehele nako e telele) 'me ha li thibele boteng ba hypersensitivity ea bongaka ho hlabang ke likokoanyana.
Ho bana, haholo-holo ho fihlela lilemo tse 2, tekanyo e tloaelehileng ea tIgE e tlase ho feta ho bacha le batho ba baholo [7]. Ka hona, ho ka lebelloa hore karolong e phahameng ea bana ba ka tlase ho lilemo tse 2 kakaretso ea boemo ba IgE e ka tlase ho moeli o boletsoeng. Moeli ona ha o sebetse ho tekanyo e itseng ea IgE.
TEBELLO MATHEO
Kamano e haufi pakeng tsa maemo a anti-IgE a khethehileng a allergen le lefu la allergic e tsebahala 'me e hlalosoa ka botlalo libukeng [1]. Mokuli e mong le e mong ea hlokofalitsoeng o tla bontša IgE profile ha e lekoa ka ALEX². Karabo ea IgE ka samptse fokolang ho tsoa ho batho ba phelang hantle bao e seng ba alejiki li tla ba ka tlase ho 0.3 kUA/l bakeng sa lintho tse sa imollang limolek'hule tse le 'ngoe le bakeng sa lintho tse qotsitsoeng ha li lekoa ka ALEX². Sebaka seo ho buuoang ka sona bakeng sa kakaretso ea IgE ho batho ba baholo ke <100 kU/l. Ts'ebetso e ntle ea laboratori e khothaletsa hore laboratori ka 'ngoe e thehe mofuta oa eona oa boleng bo lebelletsoeng.
LIEKETSENG TS'EBETSO
Litšobotsi tsa ts'ebetso hammoho le Kakaretso ea Tšireletseho le Ts'ebetso li ka fumanoa ho MADx websebaka: https://www.madx.com/extras.
TIISETSO
Lintlha tsa ts'ebetso li ile tsa fumanoa ho sebelisoa mokhoa o hlalositsoeng ho Litaelo tsena tsa Tšebeliso. Phetoho efe kapa efe kapa phetoho tšebetsong e ka ama sephetho mme MacroArray Diagnostics e latola liwaranti tsohle tse hlahisitsoeng (ho kenyeletsoa le waranti e boletsoeng ea thekiso le boikoetliso bakeng sa tšebeliso) ketsahalong e joalo. Ka lebaka leo, MacroArray Diagnostics le baphatlalatsi ba eona ba lehae ba ke ke ba ikarabella bakeng sa litšenyehelo tse sa tobang kapa tse bakiloeng ketsahalong e joalo.
TS'ELISO TS'OANELO
| ALEX | Allergy Xplorer |
| CCD | Lintho tse khethollang lik'habohaedreite tse fapaneng |
| EDTA | Ethylenediaminetetraacetic acid |
| ELISA | Tlhahlobo ea Immunosorbent e amanang le Enzyme |
| IgE | Immunoglobulin E |
| IVD | Tlhahlobo ea invitro |
| kU/l | Diyuniti tsa Kilo ka Lithara |
| kUA/l | Diyuniti tsa Kilo tsa allergen-specific IgE ka litara |
| MADx | MacroArray Diagnostics |
| REF | Nomoro ea litšupiso |
| rpm | Phallela ka motsotso |
| sIgE | IgE e khethehileng ea Allergen |
| tIgE | Kakaretso ea IgE |
| µl | Microliter |
LEthathamo la ALLERGEN ALEX²
Lihlahisoa tsa Allergen: Aca m, Aca s, Achd, Ail a, All c, All s, Ama r, Amb a, Ana o, Api m, Art v, Ave s, Ber e, Bos d meat, Bos d milk, Bro p , Cam d, Can f ♂ urine, Can s, Cap a, Cap h epithelia, Cap h milk, Car c, Car i, Car p, Che a, Che q, Chi spp., Cic a, Cit s, Cla h , Clu h, Cor a pollen, Cuc p, Cup s, Cyn d, Dau c, Dol spp., Equ c milk, Equc meat, Fag e, Fic b, Fic c, Fra e, Gad m, Gal d meat , Gal d white, Gal d yolk, Hel a, Hom g, Hor v, Jug r, Jun a, Len c, Lit s, Loc m, Lol spp., Lup a, Mac i, Man i, Mel g, Mor r, Mus a, Myt e, Ori v, Ory meat, Ory s, Ost e, Ovi a epithelia, Ovi a nama, Ovi a lebese, Pan b, Pan m, Pap s, Par j, Pas n, Pec spp. , Pen ch, Per a, Pers a, Pet c, Pha v, Phr c, Pim a, Pis s, Pla l, Pol d, Pop n, Pru av, Pru du, Pyr c, Raj c, Rat n, Rud spp., Sac c, Sal k, Sal s, Sco s, Sec c flour, Sec c pollen, Ses i, Sin, Sol spp., Sola l, Sol t, Sus d epithel, Sus d meat, Ten m, Thu a, Tri fo, Tri s, Tyr p, Ulm c, Urt d, Vac m, Ves v, Zea m flour
Likarolo tsa tlhaho tse hloekisitsoeng: nAct d 1, nApi m 1, nAra h 1, nAra 3, nBos d 4, nBos d 5, nBos d 6, nBos d 8, nCan f 3, nCor a 9, nCor a 11, nCup a 1, nCry j 1, nEqu c 3, nFag e 2, nGad m 1, nGad m 2 + 3, nGal d 2, nGal d 3, nGal d 4, nGal d 5, nGly m 5, nGly m 6, nJug r 4, nMac i 2S Albumin, nOle e 7 (RUO), nPap s 2S Albumin, nPis v 3, nPla a 2, nTri a aA_TI
Likarolo tse kopaneng: rAct d 10, rAct d 2, rAct d 5, rAln g 1, rAln g 4, rAlt a 1, rAlt a 6, rAmb a 1, rAmb a 4, rAna o 2, rAna o 3, rAni 1, rAni s 3, rApi g 1, rApi g 2, rApi g 6, rApi m 10, rAra h 2, rAra h 6, rAra h 8, rAra h 9, rAra h 15, rArg r 1, rArt v1 rAsp f 3, rAsp f 1, rAsp f 3, rAsp f 4, rBer e 6, rBet v 1, rBet v 1, rBet v 2, rBla g 6, rBla g 1, rBla g 2, rBla gla 4, 5, rBlo t 9, rBlo t 10, rBlo t 21, rBos d 5, rCan f 2, rCan f 1, rCan f 2, rCan f 4, rCan f Fel d 6 like, rCan s 1, rCav p 3, a 1, rCla h 1, rClu h 8, rCor a 1, rCor a 1.0103, rCor a 1.0401, rCor a 8 (RUO), rCor a 12, rCra c 14, , rCuc m 6, rCyp 2, rCyp 1 , rDau c 1, rDer f 1, rDer f 1, rDer p 2, rDer p 1, rDer p 10, rDer p 11, rDer p 2, rDer p 20, rDer p 21, rDer p 23, rDer p 5, c 7, rEqu c 1, rFag s 4, rFel d 1, rFel d 1, rFel d 2, rFel d 4, rFra a 7 + 1, rFra e 3, rGal d 1, rGly d mGly 1, rGly d 2, rGly m 4, rHev b 8, rHev b 1, rHev b 3, rHev b 5, rHev b 6.02, rHev b 8, rHom s LF, rJug r 11, rJug r 1, rJug r 2, 3d r Lep 6 , rLol p 2, rMal d 1, rMal d 1, rMala s 3, rMala s 11, rMala s 5, rMal d 6, rMer a 2, rMes a 1 (RUO), rMus m 1, rOle rOle 1, rOry c 1, rOry c 9, rOry c 1, rPar j 2, rPen m 3, rPen m 2, rPen m 1, rPen m 2, rPer a 3, rPhl p 4, rPhl p 7, rPhl rPhl p 1, rPhl p 12, rPhl p 2, rPho d 5.0101, rPhod s 6, rPis v 7, rPis v 2, rPis v 1 (RUO), rPla a 1, rPla a 2, rPla 4 Pol 1, , rPru p 3, rPru p 1 (RUO), rRaj c Parvalbumin, rSal k 5, rSal s 3, rSco s 7, rSes i 1, rSin a 1, rSola l 1, rSus d 1, rThu a 1, 6, rTri a 1, rTyr p 1, rVes v 14, rVes v 19, rVit v 2, rXip g 1, rZea m 5
LIEKETSENG
- Hamilton, RG. (2008). Tekolo ea mafu a alejiki ea batho. Tleliniki Immunology. 1471-1484. 10.1016/B978-0-323-04404-2.10100-9.
- Harwanegg C, Laffer S, Hiller R, Mueller MW, Kraft D, Spitzauer S, Valenta R. Microarrayed recombinant allergened bakeng sa ho hlahlojoa ha allergy. Clin Exp Allergy. 2003 Jan;33(1):7-13. doi: 10.1046/j.1365-2222.2003.01550.x. PMID: 12534543.
- Hiller R, Laffer S, Hawanegg C, Huber M, Schmidt WM, Twardosz A, Barletta B, Becker WM, Blaser K, Breiteneder H, Chapman M, Crameri R, Duchêne M, Ferreira F, Fiebig H, Hoffmann-Sommergruber K, King TP, Kleber-Janke T, Kurup VP, Lehrer SB, Lidholm J, Müller U, Pini C, Reese G, Scheiner O, Scheynius A, Shen HD, Spitzauer S, Suck R, Swoboda I, Thomas W, Tinghino R, Van Hage-Hamsten M, Virtanen T, Kraft D, Müller MW, Valenta R. Microarrayed allergenic molek'hule: ho hlahloba balebeli ba liheke bakeng sa phekolo ea ho kula. FASEB J. 2002 Mar;16(3):414-6. doi: 10.1096/fj.01-0711fje. Epub 2002 Jan 14. PMID: 11790727
- Ferrer M, Sanz ML, Sastre J, Bartra J, del Cuvillo A, Montoro J, Jáuregui I, Dávila I, Mullol J, Valero A. Tlhahlobo ea limolek'hule ka allergology: tšebeliso ea mokhoa oa microarray. J Batlisisa Allergol Clin Immunol. 2009;19 Tlatsetso 1:19-24. PMID: 19476050.
- Ott H, Fölster-Holst R, Merk HF, Baron JM. Allergen microarrays: sesebelisoa sa morao-rao bakeng sa profiling ea high-resolution IgE ho batho ba baholo ba nang le atopic dermatitis. Eur J Dermatol. 2010 Jan-Feb;20(1):54-
61. doi: 10.1684/ejd.2010.0810. Epub 2009 Oct 2. PMID: 19801343. - Sastre J. Tlhahlobo ea molek'hule ka ho kula. Clin Exp Allergy. 2010 Oct;40(10):1442-60. doi: 10.1111/j.1365-2222.2010.03585.x. Epub 2010 Aug 2. PMID: 20682003.
- Martins TB, Bandhauer ME, Bunker AM, Roberts WL, Hill HR. Linako tse ncha tsa bongoana le tsa batho ba baholo bakeng sa kakaretso ea IgE. J Allergy Clin Immunol. 2014 Feb;133(2):589-91.
Bakeng sa lintlha tse mabapi le liphuputso tsa tlhahlobo le tsa bongaka tse entsoeng li bua ka litšobotsi tsa ts'ebetso ho https://www.madx.com/extras.
FETOHA SEBAKA
| Phetolelo | Tlhaloso | E nkela sebaka |
| 11 | nGal d1 e fetotsoe ho rGal d1; URL ntjhafatswa ho madx.com; CE e tlatselletsa ka palo ea Sehlopha se Tsebisoang; fetola histori e kenyelelitsoe | 10 |
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MacroArray ALLERGY XPLORER Macro Array Diagnostics [pdf] Litaelo 91201229202JQ, 02-2001-01, 02-5001-01, ALLERGY XPLORER Macro Array Diagnostics, ALLERGY XPLORER, Macro Array Diagnostics, Array Diagnostics, Diagnostics |
