MacroArray ALLERGY XPLORER Macro Array Diagnostics
Iinkcukacha
- Igama leMveliso: Isiseko se-UDI-DI 91201229202JQ
- Amanani Ezalathiso: REF 02-2001-01, 02-5001-01
- Usetyenziso olucetywayo: Ukufunyanwa kwe-allergen-specific IgE (sIgE) ngokobungakanani kunye ne-IgE iyonke (tIgE) isiqingatha sobungakanani
- Abasebenzisi: Abasebenzi baselabhoratri abaqeqeshiweyo kunye neengcali zonyango kwilebhu yezonyango
- Ugcino: Iirejenti zeKit zizinzile kwiinyanga ezi-6 emva kokuvulwa
Imiyalelo yokusetyenziswa kwemveliso
Umgaqo weNkqubo
Imveliso ibona i-allergen-specific IgE ngokobungakanani kunye ne-IgE iyonke ngokwesiqingatha sobuninzi.
Ukuthunyelwa kunye noGcino
Qinisekisa ukuba iirejenti zekhithi zigcinwa njengoko kubonisiwe kwaye zisetyenziswa kwiinyanga ezi-6 zokuvulwa.
Ukukhupha inkunkuma:
Landela iinkqubo ezifanelekileyo zokulahla inkunkuma ngokwemigaqo.
Izixhobo zeKit
Jonga kwincwadana yomsebenzisi ngolwazi oluthe kratya kumacandelo ekhithi.
Izixhobo ezifunekayo
Uhlalutyo Lwemanyuwali: Qinisekisa ukuba unezixhobo eziyimfuneko ezibonelelwa ngumenzi.
Uhlalutyo oluzenzekelayo: Sebenzisa isixhobo se-MAX, iSisombululo sokuhlamba, iStop Solution, i-RAPTOR SERVER Analysis Software, kunye nePC/Laptop. Landela imiyalelo yesondlo ngononophelo.
Ukuphathwa kweArrays
Landela imiyalelo enikiweyo yokuphatha uluhlu ngononophelo ukuqinisekisa iziphumo ezichanekileyo.
Izilumkiso kunye neZilumkiso
- Nxiba izixhobo zokukhusela ezifanelekileyo njengokhuseleko lwesandla namehlo kunye needyasi zaselebhu.
- Ukuphatha ii-reagents kunye sampUkulandela iinkqubo ezifanelekileyo zaselabhoratri.
- Phatha zonke izixhobo zabantu njengezosulelo kwaye uziphathe ngononophelo.
FAQ
- Q: Zizinzile ixesha elingakanani ii-reagents zekhithi?
A: Ii-reagents zeKit zizinzile kwiinyanga ezi-6 emva kokuvulwa xa zigcinwe phantsi kweemeko ezibonisiweyo. - Umbuzo: Ngubani onokusebenzisa le mveliso?
A: Le mveliso yenzelwe ukusetyenziswa ngabasebenzi abaqeqeshiweyo basebhubhoratri kunye neengcali zonyango kwindawo yonyango lwebhubhoratri.
www.madx.com
UKWALIWA XPLORER (ALEX²) UMYALELO WOKUSEBENZISA
INKCAZO
I-Allergy Xplorer (ALEX²) yi-Enzyme-Linked Immunosorbent Assay (ELISA) - esekelwe kwiimvavanyo zokuxilonga kwi-vitro yobungakanani bomlinganiselo we-allergen-specific IgE (sIgE).
Lo Myalelo wokusetyenziswa uyasebenza kwezi mveliso zilandelayo:
| Isiseko se-UDI-DI | I-REF | Imveliso |
| 91201229202JQ | 02-2001-01 | I-ALEX² ye-20 Uhlalutyo |
| 02-5001-01 | I-ALEX² ye-50 Uhlalutyo |
INJONGO EKUJOLISWE KUYO
I-ALEX² I-Allergy Xplorer yikiti yovavanyo esetyenziselwa uvavanyo lwe-in-vitro lweserum yomntu okanye iplasma (ngaphandle kwe-EDTA-plasma) ukunika ulwazi ukunceda ukuxilongwa kwezigulana eziphethwe zizifo ze-IgE-mediated ngokubambisana nezinye iziphumo zovavanyo okanye iziphumo zoxilongo. .
Isixhobo sonyango se-IVD sibona i-allergen-specific IgE (sIgE) ngokobungakanani kunye ne-IgE iyonke (tIgE) i-semi-quantitatively. Imveliso isetyenziswe ngabasebenzi abaqeqeshiweyo bebhubhoratri kunye neengcali zonyango kwibhubhoratri yonyango.
ISISHWANKATHELO NENKCAZO YOVAVANYO
Ukwalana luhlobo olukhawulezileyo lwe-hypersensitivity reaction kwaye kulawulwa zizilwa-buhlungu ezizezodidi lwe-IgE lwe-immunoglobulins. Emva kokuba sesichengeni kwizinto ezithile ezimele zingabikho, ukukhutshwa kwe-histamine kunye nabanye abalamli kwiiseli zemast kunye ne-basophils kubangela ukubonakaliswa kwezonyango ezifana ne-asthma, i-rhino-conjunctivitis ye-allergic, i-atopic eczema, kunye neempawu zesisu [1]. Ke ngoko, ipateni ecacileyo yokwazisa i-allergies ethile inceda ekuvavanyeni izigulana ezingavumiyo [2-6]. Akukho mda kuvavanyo lwabemi. Xa kusenziwa iimvavanyo ze-IgE, ubudala kunye nesondo ngokuqhelekileyo azithathwa njengezinto ezibalulekileyo kuba amanqanaba e-IgE, alinganiswa kolu vavanyo, awahlukanga kakhulu ngokusekwe kolu luhlu lwabantu.
Lonke uhlobo olungundoqo lwemithombo ye-allergen lugqunywa yi-ALEX². Uluhlu olupheleleyo lwe-ALEX² ye-allergenic extracts kunye ne-molecular allergens inokufunyanwa emazantsi alo myalelo.
Ulwazi olubalulekileyo kumsebenzisi!
Ukusetyenziswa okuchanekileyo kwe-ALEX², kuyafuneka ukuba umsebenzisi afunde ngononophelo kwaye alandele le miyalelo ukuze ayisebenzise. Umvelisi akathathi tyala ngalo naluphi na ukusetyenziswa kwesi sixokelelwano sovavanyo esingachazwanga kolu xwebhu okanye uhlengahlengiso ngumsebenzisi wenkqubo yovavanyo.
Ingqalelo: Umahluko wekhithi 02-2001-01 yovavanyo lwe-ALEX² (i-20 Arrays) yenzelwe ngokukodwa ukusetyenzwa ngesandla. Ukusebenzisa olu tshintsho lwekhithi ye-ALEX² kunye ne-automated MAX 9k, iSisombululo sokuhlamba (REF 00-5003-01) kunye neStop Solution (REF 00-5007-01) kufuneka ziodolwe ngokwahlukeneyo. Lonke ulwazi olongezelelweyo lwemveliso lunokufumaneka kwimiyalelo ehambelanayo yokusetyenziswa: https://www.madx.com/extras.
Umahluko wekhithi ye-ALEX² 02-5001-01 (uluhlu oluyi-50) lunokusetyenziselwa ukusetyenzwa ngokuzenzekelayo nge-MAX 9k (REF 17-0000-01) kunye nesixhobo se-MAX 45k (REF 16-0000-01).
UMGAQO WENKQUBO
I-ALEX² luvavanyo lwe-immunoassay olusekwe kwi-Enzyme-Linked Immunosorbent Assay (ELISA). Izicatshulwa ze-Allergen okanye i-molecular allergens, ezidityaniswe ne-nanoparticles, zifakwe ngendlela ecwangcisiweyo kwisigaba esiqinileyo esenza uluhlu lwe-macroscopic. Okokuqala, i-particle-bound allergens isabela nge-IgE ethile ekhoyo kwisigulaneample. Emva kofukamo, i-IgE engangcaciswanga iyahlanjwa iphele. Inkqubo iyaqhubeka ngokongeza i-enzyme-ileyibhile i-anti-human IgE yokufumanisa i-antibody eyenza i-complex kunye ne-particle-bound i-IgE ethile. Emva kwesinyathelo sesibini sokuhlamba, i-substrate yongezwa eguqulwa ibe yi-insoluble, i-precipitate enemibala yi-enzyme eboshwe kwi-antibody. Ekugqibeleni, i-enzyme-substrate reaction iyeka ngokudibanisa i-reagent blocking. Ubungakanani bemvula ilingana noxinaniso lwe-IgE ethile kwisgulanaample. Inkqubo yovavanyo lwaselebhu ilandelwa kukufumana umfanekiso kunye nohlalutyo kusetyenziswa nokuba yi-manual system (ImageXplorer) okanye inkqubo ezenzekelayo (MAX 45k okanye MAX 9k). Iziphumo zovavanyo zihlalutywa nge-RAPTOR SERVER Analysis Software kwaye zichazwe kwiiyunithi zokuphendula ze-IgE (kUA/l). Itotali yeziphumo ze-IgE nazo zichazwe kwiiyunithi zokuphendula ze-IgE (kU/l). I-RAPTOR SERVER iyafumaneka kuguqulelo 1, ngenombolo epheleleyo enedijithi ezine nceda ujonge kushicilelo lwe-RAPTOR SERVER olufumanekayo www.raptor-server.com/imprint.
UKUTHUMELA KUNYE NOGCINWA
Ukuthunyelwa kwe-ALEX² kwenzeka kwiimeko zobushushu obuphakathi. Nangona kunjalo, ikiti kufuneka igcinwe ngokukhawuleza ekuhanjisweni kwe-2-8 ° C. Igcinwe ngokuchanekileyo, i-ALEX² kunye nezixhobo zayo zingasetyenziswa kude kube ngumhla ochaziweyo wokuphelelwa.
Ii-reagents zeKit zizinzile kwiinyanga ezili-6 emva kokuvulwa (kwiimeko zokugcina ezibonisiweyo).
UKUKHUPHA INKUNKUMA
Lahla ikhatriji ye-ALEX² esetyenzisiweyo kunye nezixhobo zekhithi ezingasetyenziswanga kunye nenkunkuma yeekhemikhali zaselabhoratri. Landela yonke imimiselo yesizwe, yelizwe, neyengingqi malunga nokulahlwa.
Uluhlu lwamagama eempawu
AMANQAKU EKIT
Icandelo ngalinye (i-reagent) lizinzile kude kube ngumhla ochazwe kwileyibhile yecandelo ngalinye. Akukhuthazwa ukuba udibanise naziphi na ii-reagents ezivela kwiikiti ezahlukeneyo. Ngoluhlu lwezicatshulwa ze-allergen kunye ne-molecular allergens ezingashukumiyo kuluhlu lwe-ALEX², nceda uqhagamshelane support@madx.com.
| Izixhobo zeKit REF 02-2001-01 | Umxholo | Iipropati |
| I-ALEX² Ikhatriji | 2 Amadyungudyungu à 10 ALEX² kuhlalutyo lwe-20 lulonke.
Ulungelelwaniso nge-master curve efumaneka ngeRAPTOR SERVER Uhlalutyo lweSoftware. |
Ukulungele ukusetyenziswa. Gcina kwi-2-8 ° C kude kube ngumhla wokuphelelwa yisikhathi. |
| I-ALEX² Sample Diluent | Ibhotile enye kwi-1 ml | Ukulungele ukusetyenziswa. Gcina kwi-2-8 ° C kude kube ngumhla wokuphelelwa yisikhathi. Vumela i-reagent ukuba ifikelele kubushushu begumbi ngaphambi kokusetyenziswa. I-reagent evuliwe izinzile kwiinyanga ezi-6 kwi-2-8 ° C, iquka i-CCD inhibitor. |
| Ukuhlamba Isisombululo | Ibhotile enye kwi-2 ml | Ukulungele ukusetyenziswa. Gcina kwi-2-8 ° C kude kube ngumhla wokuphelelwa yisikhathi. Vumela i-reagent ukuba ifikelele kubushushu begumbi ngaphambi kokusetyenziswa. I-reagent evuliwe izinzile kwiinyanga ezili-6 kwi-2-8 ° C. |
| Izixhobo zeKit REF 02-2001-01 | Umxholo | Iipropati |
| I-ALEX² i-Antibody yokuFumana | Ibhotile enye kwi-1 ml | Ukulungele ukusetyenziswa. Gcina kwi-2-8 ° C kude kube ngumhla wokuphelelwa yisikhathi. Vumela i-reagent ukuba ifikelele kubushushu begumbi ngaphambi kokusetyenziswa. I-reagent evuliwe izinzile kwiinyanga ezili-6 kwi-2-8 ° C. |
| ALEX² Isisombululo seSubstrate | Ibhotile enye kwi-1 ml | Ukulungele ukusetyenziswa. Gcina kwi-2-8 ° C kude kube ngumhla wokuphelelwa yisikhathi. Vumela i-reagent ukuba ifikelele kubushushu begumbi ngaphambi kokusetyenziswa. I-reagent evuliwe izinzile kwiinyanga ezili-6 kwi-2-8 ° C. |
| (ALEX²) Misa Isisombululo | Ibhotile enye kwi-1 ml | Ukulungele ukusetyenziswa. Gcina kwi-2-8 ° C kude kube ngumhla wokuphelelwa yisikhathi. Vumela i-reagent ukuba ifikelele kubushushu begumbi ngaphambi kokusetyenziswa. I-reagent evuliwe izinzile kwiinyanga ezi-6 kwi-2-8 ° C. Inokuvela njengesisombululo se-turbid emva kokugcinwa ixesha elide. Oku akunampembelelo kwiziphumo. |
| Izixhobo zeKit REF 02-5001-01 | Umxholo | Iipropati |
| I-ALEX² Ikhatriji | 5 Amadyungudyungu à 10 ALEX² kuhlalutyo lwe-50 lulonke.
Ulungelelwaniso nge-master curve efumaneka ngeRAPTOR SERVER Analysis Software. |
Ukulungele ukusetyenziswa. Gcina kwi-2-8 ° C kude kube ngumhla wokuphelelwa yisikhathi. |
| I-ALEX² Sample Diluent | Ibhotile enye kwi-1 ml | Ukulungele ukusetyenziswa. Gcina kwi-2-8 ° C kude kube ngumhla wokuphelelwa yisikhathi. Vumela i-reagent ukuba ifikelele kubushushu begumbi ngaphambi kokusetyenziswa. I-reagent evuliwe izinzile kwiinyanga ezi-6 kwi-2-8 ° C, iquka i-CCD inhibitor. |
| Ukuhlamba Isisombululo | 4 x ikhonkco. 1 ibhotile ukuya 250 ml | Gcina kwi-2-8 ° C kude kube ngumhla wokuphelelwa yisikhathi. Nciphisa i-1 ukuya kwi-4 ngamanzi adityanisiweyo ngaphambi kokusetyenziswa. Vumela i-reagent ukuba ifikelele kubushushu begumbi ngaphambi kokusetyenziswa. I-reagent evuliwe izinzile kwiinyanga ezi-6 kwi-2-8 ° C. |
| I-ALEX² i-Antibody yokuFumana | Ibhotile enye kwi-1 ml | Ukulungele ukusetyenziswa. Gcina kwi-2-8 ° C kude kube ngumhla wokuphelelwa yisikhathi. Vumela i-reagent ukuba ifikelele kubushushu begumbi ngaphambi kokusetyenziswa. I-reagent evuliwe izinzile kwiinyanga ezili-6 kwi-2-8 ° C. |
| Izixhobo zeKit REF 02-5001-01 | Umxholo | Iipropati |
| ALEX² Isisombululo seSubstrate | Ibhotile enye kwi-1 ml | Ukulungele ukusetyenziswa. Gcina kwi-2-8 ° C kude kube ngumhla wokuphelelwa yisikhathi. Vumela i-reagent ukuba ifikelele kubushushu begumbi ngaphambi kokusetyenziswa. I-reagent evuliwe
uzinzile kangangeenyanga ezi-6 kwi-2-8°C. |
| (ALEX²) Misa Isisombululo | Ibhotile enye kwi-1 ml | Ukulungele ukusetyenziswa. Gcina kwi-2-8 ° C kude kube ngumhla wokuphelelwa yisikhathi. Vumela i-reagent ukuba ifikelele kubushushu begumbi ngaphambi kokusetyenziswa. I-reagent evuliwe izinzile kwiinyanga ezi-6 kwi-2-8 ° C. Inokuvela njengesisombululo se-turbid emva kokugcinwa ixesha elide. Oku akunampembelelo kwiziphumo. |
IZIXHOBO EZIFUNEKAYO UKUQHUBEKA NOKUHLALUTYA
Uhlalutyo Lwemanyuwali
- ImageXplorer
- Isixhasi (uyazikhethela)
- ILab Rocker (i-engile yotyekelo 8°, isantya esifunekayo 8 rpm)
- Igumbi lokufukamela (WxDxH – 35x25x2 cm)
- I-RAPTOR SERVER Analysis Software
- PC/Laptop
Izixhobo ezifunekayo, ezingabonelelwanga yi-MADx:
- Amanzi athotyiweyo
- IiPipettes & iingcebiso (100 µl & 100 - 1000 µl)
Uhlalutyo oluzenzekelayo:
- MAX isixhobo (MAX 45k okanye MAX 9k)
- Isisombululo sokuhlamba (REF 00-5003-01)
- Misa isisombululo (REF 00-5007-01)
- I-RAPTOR SERVER Analysis Software
- PC/Laptop
Iinkonzo zogcino ngokwemiyalelo yomenzi.
UKUPHATHWA KWE-ARRAYS
Musa ukubamba umphezulu woluhlu. Naziphi na iziphene ezingaphezulu ezibangelwa zizinto ezinqabileyo okanye ezibukhali zingaphazamisa ukufunda okuchanekileyo kweziphumo. Sukuyifumana imifanekiso ye-ALEX² phambi kokuba uluhlu lome ngokupheleleyo (lome kwiqondo lobushushu begumbi).
IZILUMKISO NEMIGAQO
- Kuyacetyiswa ukuba unxibe ukhuseleko lwezandla kunye namehlo kunye needyasi zaselebhu kwaye ulandele iinkqubo ezifanelekileyo zaselabhoratri xa ulungiselela kwaye uphatha iirejenti kunye s.amples.
- Ngokuhambelana nenkqubo efanelekileyo yelabhoratri, zonke izinto ezisetyenziswa ngabantu kufuneka zithathelwe ingqalelo njengosulelo kwaye ziphathwe ngendlela yolumkeleko efana neyomguli.amples.
- I-ALEX² Sampi-le Diluent kunye ne-Washing Solution iqulethe i-azide ye-sodium (<0.1%) njengesigcini kwaye kufuneka iphathwe ngononophelo. Iphepha ledatha yokhuseleko liyafumaneka xa uceliwe.
- I (ALEX²) Stop Solution iqulethe i-Ethylenediaminetetraacetic acid (EDTA)-Isisombululo kwaye kufuneka iphathwe ngononophelo. Iphepha ledatha yokhuseleko liyafumaneka xa uceliwe.
- Ukusetyenziswa kwe-in-vitro diagnostic kuphela. Ayinakusetyenziswa ngaphakathi okanye ngaphandle ebantwini okanye kwizilwanyana.
- Kuphela ngabasebenzi abaqeqeshelwe ukusebenza elabhoratri ekufuneka basebenzise le khithi.
- Ekufikeni, khangela amacandelo ekhithi umonakalo. Ukuba elinye lamacandelo lonakele (umz. iibhotile ze-buffer), qhagamshelana ne-MADx (support@madx.com) okanye umsasazi wakho wendawo. Musa ukusebenzisa izixhobo zekiti ezonakalisiweyo, njengoko ukusetyenziswa kwazo kunokukhokelela ekusebenzeni kakubi kwekiti.
- Musa ukusebenzisa ii-reagents ngaphaya kwemihla yazo yokuphelelwa yisikhathi.
- Musa ukuxuba ii-reagents ezivela kwiibhetshi ezahlukeneyo.
ELISA INKQUBO
Ukulungiselela
Ukulungiswa kwesampLes: I-Serum okanye i-plasma (i-heparin, i-citrate, akukho EDTA) sampIgazi le-capillary okanye le-venous lingasetyenziswa. Igazi samples zinokuqokelelwa kusetyenziswa iinkqubo eziqhelekileyo. Gcina sampngaphantsi kwe-2-8 ° C ukuya kwiveki enye. Gcina i-serum kunye ne-plasma sampngaphantsi kwe -20°C ukugcinwa ixesha elide. Ukuthunyelwa kwe-serum/plasma sampUbushushu begumbi buyasebenza. Hlala uvumela i-sampUkufikelela kubushushu begumbi ngaphambi kokusetyenziswa.
Ukulungiswa kwesisombululo sokuhlamba (kuphela kwi-REF 02-5001-01 kunye ne-REF 00-5003-01 xa isetyenziswe ngesixhobo se-MAX): Galela umxholo we-1 vial ye-Washing Solution kwi-container yokuhlamba isixhobo. Gcwalisa amanzi adityanisiweyo ukuya kuphawu olubomvu kwaye uxube ngokucophelela isikhongozeli ngamaxesha amaninzi ngaphandle kokuvelisa amagwebu. I-reagent evuliwe izinzile kwiinyanga ezi-6 kwi-2-8 ° C.
Igumbi lokufukamela: Vala isiciko kuwo onke amanyathelo okuvavanya ukuthintela ukuhla kokufuma.
Iiparamitha of Inkqubo:
- 100µlample + 400 µl I-ALEX² Sample Diluent
- 500 µl ALEX² I-Antibody yokuFumana
- 500 µl ALEX² Isisombululo seSubstrate
- 100 µl (ALEX²) Misa Isisombululo
- 4500 µl Isisombululo sokuhlamba
Ixesha lovavanyo limalunga ne-3 h 30 min (ngaphandle kokoma koluhlu olucwangcisiweyo).
Akukhuthazwa ukuba kuqhutywe iimvavanyo ezininzi kunokuba kunokutshizwa ngombhobho kwi-8 min. Zonke ii-incubations zenziwa kwiqondo lobushushu begumbi, 20-26°C.
Zonke ii-reagents kufuneka zisetyenziswe kwindawo yokushisa (20-26 ° C). Uvavanyo akufanele lwenziwe kwilanga elithe ngqo.
Lungisa igumbi lokufukamela
Vula igumbi lokufukamela kwaye ubeke iitawuli zephepha kwindawo engezantsi. Gxuma iitawuli zephepha ngamanzi adityanisiweyo de kungabikho ndawo ezomileyo zeetawuli zephepha ezibonakalayo.
Sample incubation/CCD inhibition
Thatha inani elifunekayo leekhatriji ze-ALEX² kwaye uzibeke kwisibambi soluhlu. Yongeza i-400 μl ye-ALEX² Sample Diluent kwikhatriji nganye. Yongeza i-100 μl yesigulana sample kwiikhatriji. Qinisekisa ukuba isisombululo esisiphumo sisasazwa ngokulinganayo. Beka iikhatriji kwigumbi lokufukamela elilungisiweyo kwaye ubeke igumbi lokufukamela kunye neekhatriji kwi-lab rocker ukuze iikhatriji zishukume kwicala elide lekhatriji. Qalisa i-serum incubation nge-8 rpm kwiiyure ezi-2. Vala igumbi lokufukamela phambi kokuba uqalise ilebhu rocker. Emva kweeyure ze-2, khupha i-samples kwisikhongozeli senkongozelo. Cima ngononophelo amathontsi kwikhatriji usebenzisa itawuli yephepha.
Kuphephe ukubamba umphezulu woluhlu kunye netawuli yephepha! Kuphephe nakuphi na ukuthwala okanye ukungcoliseka kwesampkancinci phakathi kweekhatriji ze-ALEX²!
Ngokuzithandela okanye elungileyo Hom s LF (CCD marker): kunye neprothokholi esemgangathweni yeCCD ye-antibody inhibition (njengoko kuchaziwe kumhlathi 2: sample incubation/CCD inhibition) iCCD inhibition esebenzayo yi 85%. Ukuba izinga eliphezulu lokuvinjelwa lifunekayo, lungiselela i-1 ml sample ityhubhu, yongeza 400 μl ALEX² Sample Diluent kunye ne-100 μl serum. Fuka imizuzu engama-30 (ungangcangcazeli) uze uqhubeke nenkqubo yovavanyo eqhelekileyo.
Phawula: Inyathelo elithe kratya lothintelo lweCCD likhokelela kwiimeko ezininzi kumyinge wothintelo kwizilwa-buhlungu zeCCD ezingaphezu kwama-95%.
1a. Ukuhlamba I
Yongeza i-500 μl yokuWashing Solution kwikhatriji nganye kwaye uqandusele kwi-lab rocker (ngo-8 rpm) imizuzu emi-5. Khipha iSisombululo sokuhlamba kwisitya sokuqokelela kwaye ucofe ngamandla iikhatriji kwi-stack yeetawuli zephepha ezomileyo. Cima ngononophelo amathontsi aseleyo kwiikhatriji usebenzisa itawuli yephepha.
Phinda eli nyathelo 2 amaxesha amaninzi.
Yongeza i-antibody
Yongeza i-500 µl ye-ALEX² i-Antibody yokuFumana kwikhatriji nganye.
Qinisekisa ukuba uluhlu olupheleleyo lomphezulu lugqunywe sisisombululo se-ALEX² sokufunyanwa kwe-Antibody.
Beka iikhatriji kwi-incubation chamber kwi-lab rocker kwaye udibanise kwi-8 rpm imizuzu engama-30. Khupha isisombululo se-Detection Antibody kwisitya sokuqokelela kwaye ucofe ngamandla iikhatriji kwisipaki seetawuli zephepha ezomileyo. Cima ngononophelo amathontsi aseleyo kwiikhatriji usebenzisa itawuli yephepha.
2a. Ukuhlamba II
Yongeza i-500 μl yokuWashing Solution kwikhatriji nganye kwaye uqandusele kwi-lab rocker ngo-8 rpm imizuzu emi-5. Khipha iSisombululo sokuhlamba kwisitya sokuqokelela kwaye ucofe ngamandla iikhatriji kwi-stack yeetawuli zephepha ezomileyo. Cima ngononophelo amathontsi aseleyo kwiikhatriji usebenzisa itawuli yephepha.
Phinda eli nyathelo 4 amaxesha amaninzi.
3+4. Yongeza i-ALEX² Isisombululo seSubstrate kwaye uyeke ukusabela kwi-substrate
Yongeza i-500 μl ye-ALEX² Substrate Solution kwikhatriji nganye. Qala i-timer ngokuzalisa i-cartridge yokuqala kwaye uqhubeke nokuzaliswa kweekhatriji eziseleyo. Qinisekisa ukuba uluhlu olupheleleyo lomphezulu lugqunywe siSisombululo se-Substrate kwaye ufukame uluhlu lwemizuzu eyi-8 ngqo ngaphandle kokungcangcazela (i-lab rocker kwi-0 rpm kwaye kwindawo ethe tye).
Emva kwemizuzu esi-8 kanye, yongeza i-100 μl ye (ALEX²) Stop Solution kuzo zonke iikhatriji, uqale ngekhatriji yokuqala ukuqinisekisa ukuba zonke iiarray zifukanyelwa ngexesha elinye kunye ne-ALEX² Substrate Solution. Vuma ngononophelo ukuze usasaze ngokulinganayo (ALEX²) Misa Isisombululo kwiikhatriji zoluhlu, emva kokuba (ALEX²) Isisombululo sokuMisa sifakwe ngombhobho kuzo zonke iindawo. Emva koko khupha i-(ALEX²) iSubstrate/Stop Solution kwiikhatriji kwaye ucofe ngamandla iikhatriji kwisipaki seetawuli zephepha ezomileyo. Sula ngokucophelela nawaphi na amathontsi asele kwiikhatriji usebenzisa itawuli yephepha.
ILab Rocker AKUMAKUFUNEKA IZINIKEZE ngexesha lokuqandusela i-substrate!
4a. Ukuhlamba III
Yongeza i-500 μl yokuWashing Solution kwikhatriji nganye kwaye uqandusele kwi-lab rocker ngo-8 rpm imizuzwana engama-30. Khipha iSisombululo sokuhlamba kwisitya sokuqokelela kwaye ucofe ngamandla iikhatriji kwi-stack yeetawuli zephepha ezomileyo. Sula ngokucophelela nawaphi na amathontsi asele kwiikhatriji usebenzisa itawuli yephepha.
Uhlalutyo lomfanekiso
Emva kokugqiba inkqubo yokuvavanya, umoya womise ii-arrays kwiqondo lokushisa eliqhelekileyo de kube yome ngokupheleleyo (ingathatha ukuya kwi-45 min).
Ukomisa ngokupheleleyo kubalulekile kubuntununtunu bovavanyo. Kuphela ii-arrays ezomiswe ngokupheleleyo zibonelela ngophawu olufanelekileyo kumlinganiselo wengxolo.
Ekugqibeleni, iindawo ezomileyo ziskenwa nge-ImageXplorer okanye isixhobo se-MAX kwaye zihlalutywe nge-RAPTOR SERVER Analysis software (jonga iinkcukacha kwi-RAPTOR SERVER software handbook). I-RAPTOR SERVER Analysis Software iqinisekiswa kuphela ngokudibanisa nesixhobo se-ImageXplorer kunye nezixhobo ze-MAX, ngoko ke i-MADx ayithathi naluphi na uxanduva lweziphumo, ezifunyenwe kunye nasiphi na esinye isixhobo sokuthatha umfanekiso (njengama-scanners).
Ulungelelwaniso loVavanyo
I-ALEX² ijiko lolungelelwaniso elibalaseleyo lasekwa ngovavanyo lwereferensi ngokuchasene nolungiselelo lweserum ene-IgE ethile ngokuchasene neeantigens ezahlukeneyo ezigquma uluhlu olucetyiweyo lokulinganisa. Uninzi lweeparamitha ezithile zokulinganisa zibonelelwa yiRAPTOR SERVER Analysis Software. Iziphumo zovavanyo lwe-ALEX² sigE zibonakaliswa njenge-kUA/l. Itotali yeziphumo ze-IgE zi-semi-quantitative kwaye zibalwa ukusuka kumlinganiselo we-anti-IgE kunye nezinto ezichanekileyo zokulinganisa, ezibonelelwa yi-RAPTOR SERVER Analysis Software kwaye zikhethwe ngokwe-lot-specific QR-codes.
Iiparamitha zeCurve kwiqashiso ngalinye zihlengahlengiswa yinkqubo yovavanyo lwereferensi yangaphakathi, ngokuchasene namalungiselelo e-serum avavanyiweyo kwi-ImmunoCAP (Thermo Fisher Scientific) ye-IgE ethile ngokuchasene nezinto ezininzi ezingavumiyo. Iziphumo ze-ALEX² ke ngoko zilandeleka ngokungangqalanga ngokuchasene nolungiselelo lwereferensi ye-WHO 11/234 ye-IgE iyonke.
Iiyantlukwano ezicwangcisiweyo kumanqanaba omqondiso phakathi kwamaqashiso zenziwa ziqheleke ngolungelelwaniso lwe-heterologous ngokuchasene ne-IgE yereferensi ijiko. Umba wokulungisa usetyenziselwa ukulungelelanisa ngokwenkqubo ukunxaxha komlinganiselo othile.
Uluhlu lokulinganisa
I-IgE ecacileyo: 0.3-50 kUA / l ubungakanani
I-IgE iyonke: 20-2500 kU/l isiqingatha sobungakanani
ULAWULO LWEMEKO
Ukugcinwa kweerekhodi kuvavanyo ngalunye
Ngokoqheliselo olululo lwaselabhoratri kuyacetyiswa ukuba urekhode amanani eqashiso azo zonke ii-reagents ezisetyenzisiweyo.
Imizekelo yokulawula
Ngokwendlela yokusebenza efanelekileyo kwilabhoratri kucetyiswa ukuba ulawulo lomgangatho sampLes zibandakanyiwe ngaphakathi kwezithuba ezichaziweyo. Amaxabiso okubhekisa kwisera ezithile ezifumanekayo zolawulo zinokubonelelwa yi-MADx xa ucelile.
UKUHLALUTYWA KWEDATHA
Kuhlalutyo lwemifanekiso yee-arrays ezicwangcisiweyo, i-ImageXplorer okanye isixhobo se-MAX siza kusetyenziswa. I-ALEX² imifanekiso ihlalutywa ngokuzenzekelayo kusetyenziswa iRAPTOR SERVER Analysis Software kwaye ingxelo iye yenziwa ishwankathela iziphumo zomsebenzisi.
IZIPHUMO
I-ALEX² luvavanyo lobungakanani be-ELISA kwi-IgE ethile kunye ne-semi-quantitative method ye-IgE iyonke. Izilwa-buhlungu ze-Allergen-specific IgE zibonakaliswa njengeeyunithi zokuphendula ze-IgE (kUA/l), zizonke iziphumo ze-IgE njenge-kU/l. I-RAPTOR SERVER Analysis Software ibala ngokuzenzekelayo kwaye inike ingxelo ngeziphumo ze-sIgE (ngokobungakanani) kunye neziphumo ze-tIgE (nge-semi-quantitatively).
IMIDA YENKQUBO
Ukuxilongwa okucacileyo kweklinikhi kufuneka kwenziwe kuphela ngokubambisana nazo zonke iziphumo ezifumanekayo zeklinikhi ngabasebenzi bezonyango kwaye akuyi kusekelwe kwiziphumo zendlela enye yokuxilongwa kuphela.
Kwiindawo ezithile zokusetyenziswa (umz. ukwaliwa kokutya), izilwa-buhlungu ezijikelezayo ze-IgE zisenokuhlala zingabonakali nangona ukubonakaliswa kweklinikhi kokungavisisani kokutya okuchasene ne-allergen ethile kunokubakho, kuba ezi zithinteli-zifo zinokuba zizodwa kwizinto ezimele zihlaziywe ngexesha lokwenziwa kwemizi-mveliso, ukupheka okanye ukwetyisa. kwaye ngenxa yoko azikho ekutyeni kwantlandlolo ekuvavanyelwe kona isigulana.
Iziphumo ezimbi zetyhefu zibonisa kuphela amanqanaba angabonakaliyo e-antibodies ye-IgE yetyhefu (umz. ngenxa yokungatyhileki ixesha elide) kwaye ayikuthinteli ubukho bekliniki hypersensitivity kwizinambuzane.
Ebantwaneni, ngakumbi ukuya kuthi ga kwiminyaka emi-2 ubudala, uluhlu oluqhelekileyo lwe-tigE lungaphantsi kunelolutsha kunye nabantu abadala [7]. Ngoko ke, kulindeleke ukuba kumlinganiselo ophezulu wabantwana abangaphantsi kweminyaka emi-2 i-IgE-level iyonke ilele ngaphantsi komda ochaziweyo wokufumanisa. Lo mda awusebenzi kumlinganiselo othile we-IgE.
IINDLELA EZILINDELEKILEYO
Unxulumano olusondeleyo phakathi kwe-allergen-specific IgE antibody amanqanaba kunye nesifo somzimba owaziwayo kwaye uchazwa ngokucokisekileyo kuncwadi [1]. Isigulana ngasinye esisivayo siya kubonisa i-IgE profile xa ivavanywa nge-ALEX². Impendulo ye-IgE nge-sampOkuncinci kubantu abasempilweni abangaphambanisiyo kuya kuba ngaphantsi kwe-0.3 kUA/l kwimolekyuli enye yezinto ezimele ukuxakwa kunye nezicatshulwa ze-allergen xa zivavanywa nge-ALEX². Indawo ekubhekiselwa kuyo i-IgE iyonke kubantu abadala yi<100 kU/l. Ukuziqhelanisa nelabhoratri elungileyo kucebisa ukuba ilabhoratri nganye iseke uluhlu lwayo lwamaxabiso alindelekileyo.
IIMPAWU ZOKUSEBENZA
Iimpawu zokusebenza kunye nesiShwankathelo soKhuseleko kunye nokuSebenza kunokufumaneka kwi-MADx webindawo: https://www.madx.com/extras.
ISIQINISEKISO
Idatha yokusebenza ifunyenwe kusetyenziswa inkqubo echazwe kule Miyalelo yokusetyenziswa. Naluphi na utshintsho okanye ukuguqulwa kwenkqubo kunokuchaphazela iziphumo kwaye i-MacroArray Diagnostics ikhupha zonke iziqinisekiso ezichaziweyo (kubandakanywa newaranti echaziweyo yokuthengiswa kunye nokufaneleka ukusetyenziswa) kweso siganeko. Ngenxa yoko, iMacroArray Diagnostics kunye nabasasazi bayo basekhaya abayi kuba nabutyala bomonakalo ongathanga ngqo okanye oyimfuneko kwisehlo esinjalo.
USHWANKATHELO
| ALEX | I-Allergy Xplorer |
| CCD | Izikhombisi ze-carbohydrate ezinqamlezayo |
| I-EDTA | Ethylenediaminetetraacetic acid |
| ELISA | Uvavanyo lwe-Enzyme-Linked Immunosorbent |
| IgE | Immunoglobulin E |
| IVD | In-vitro diagnostic |
| kU/l | Iiyunithi zeKilo ngeLitha nganye |
| kUA/l | Iiyunithi zeKilo ze-allergen-specific IgE ilitha nganye |
| MADx | MacroArray Diagnostics |
| I-REF | Inombolo yerefurensi |
| rpm | Ukujikeleza ngomzuzu |
| sigE | I-Allergen-specific IgE |
| tIgE | Iyonke IgE |
| .l | Microliter |
ULUHLU LWE-ALEX²
Izicatshulwa ze-Allergen: Aca m, Aca s, Achd, Ail a, All c, All s, Ama r, Amb a, Ana o, Api m, Art v, Ave s, Ber e, Bos d meat, Bos d milk, Bro p , Cam d, Can f ♂ umchamo, Can s, Cap a, Cap h epithelia, Cap h milk, Car c, Car i, Car p, Che a, Che q, Chi spp., Cic a, Cit s, Cla h , Clu h, Cor a pollen, Cuc p, Cup s, Cyn d, Dau c, Dol spp., Equ c milk, Equ c inyama, Fag e, Fic b, Fic c, Fra e, Gad m, Gal d inyama , Gal d white, Gal d yolk, Hel a, Hom g, Hor v, Jug r, Jun a, Len c, Lit s, Loc m, Lol spp., Lup a, Mac i, Man i, Mel g, Mor r, Mus a, Myt e, Ori v, Ory meat, Ory s, Ost e, Ovi a epithelia, Ovi a meat, Ovi a milk, Pan b, Pan m, Pap s, Par j, Pas n, Pec spp. , Pen ch, Per a, Pers a, Pet c, Pha v, Phr c, Pim a, Pis s, Pla l, Pol d, Pop n, Pru av, Pru du, Pyr c, Raj c, Rat n, Rud spp., Sac c, Sal k, Sal s, Sco s, Sec c powder, Sec c pollen, Ses i, Sin, Sol spp., Sola l, Sol t, Sus d epithel, Sus d meat, Ten m, Thu. a, Tri fo, Tri s, Tyr p, Ulm c, Urt d, Vac m, Ves v, Zea m umgubo
Amalungu endalo asulungekileyo: nAct 1, nApi m 1, nAra h 1, nAra 3, nBos d 4, nBos d 5, nBos d 6, nBos d 8, nCan f 3, nCor a 9, nCor a 11, nCup a 1, nCry j 1, nEqu c 3, nFag e 2, nGad m 1, nGad m 2 + 3, nGal d 2, nGal d 3, nGal d 4, nGal d 5, nGly m 5, nGly m 6, nJug r 4, nMac i 2S Albumin, nOle e 7 (RUO), nPap s 2S Albumin, nPis v 3, nPla a 2, nTri a aA_TI
Amalungu adibeneyo: Isenzo d 10, rAct d 2, rAct d 5, rAln g 1, rAln g 4, rAlt a 1, rAlt a 6, rAmb a 1, rAmb a 4, rAna o 2, rAna o 3, rAni Ani 1 3, rApi g 1, rApi g 2, rApi g 6, rApi m 10, rAra h 2, rAra h 6, rAra h 8, rAra h 9, rAra h 15, rArg r 1, rAra 1, rArt v3 rAsp f 1, rAsp f 3, rAsp f 4, rAsp f 6, rBer e 1, rBet v 1, rBet v 2, rBet v 6, rBla g 1, rBla g 2, rBla g 4, rBla gla 5, 9, rBlo t 10, rBlo t 21, rBlo t 5, rBos d 2, rCan f 1, rCan f 2, rCan f 4, rCan f 6, rCan f Fel d 1 like, rCan s 3, rCav p 1, a 1, rCla h 8, rClu h 1, rCor a 1.0103, rCor a 1.0401, rCor a 8, rCor a 12 (RUO), rCor a 14, rCra c 6, , rCuc m 2, rCyp1, rCyp1 , rDau c 1, rDer f 1, rDer f 2, rDer p 1, rDer p 10, rDer p 11, rDer p 2, rDer p 20, rDer p 21, rDer p 23, rDer p 5, rDer p 7, c 1, rEqu c 4, rFag s 1, rFel d 1, rFel d 2, rFel d 4, rFel d 7, rFra a 1 + 3, rFra e 1, rGal d 1, rGly d 2, rGly m 4, rHev b 8, rHev b 1, rHev b 3, rHev b 5, rHev b 6.02, rHev b 8, rHom s LF, rJug r 11, rJug r 1, rJug r 2, 3d 6r. , rLol p 2, rMal d 1, rMal d 1, rMala s 3, rMala s 11, rMala s 5, rMal d 6, rMer a 2, rMes a 1 (RUO), rMus m 1, rOle rOle 1, rOry c 1, rOry c 9, rOry c 1, rPar j 2, rPen m 3, rPen m 2, rPen m 1, rPen m 2, rPer a 3, rPhl p 4, rPhl p 7, rPhl rPhl p 1, rPhl p 12, rPhl p 2, rPho d 5.0101, rPhod s 6, rPis v 7, rPis v 2, rPis v 1 (RUO), rPla a 1, rPla a 2, rPla 4 Pol 1, , rPru p 3, rPru p 1 (RUO), rRaj c Parvalbumin, rSal k 5, rSal s 3, rSco s 7, rSes i 1, rSin a 1, rSola l 1, rSus d 1, rThuri a1, 6, rTri a 1, rTyr p 1, rVes v 14, rVes v 19, rVit v 2, rXip g 1, rZea m 5
IZIQINISEKISO
- Hamilton, RG. (2008). Uvavanyo lwezifo ezibangelwa kukwalana kwabantu. I-Clinical Immunology. 1471-1484. 10.1016/B978-0-323-04404-2.10100-9.
- I-Harwanegg C, i-Laffer S, i-Hiller R, i-Mueller MW, i-Kraft D, i-Spitzauer S, i-Valenta R. I-Microarrayed recombinant allergened yokuxilongwa kwe-allergies. I-Clin Exp Yokwaliwa. 2003 Jan;33(1):7-13. doi: 10.1046/j.1365-2222.2003.01550.x. PMID: 12534543.
- Hiller R, Laffer S, Harwanegg C, Huber M, Schmidt WM, Twardosz A, Barletta B, Becker WM, Blaser K, Breiteneder H, Chapman M, Crameri R, Duchêne M, Ferreira F, Fiebig H, Hoffmann-Sommergruber K, King TP, Kleber-Janke T, Kurup VP, Lehrer SB, Lidholm J, Müller U, Pini C, Reese G, Scheiner O, Scheynius A, Shen HD, Spitzauer S, Suck R, Swoboda I, Thomas W, Tinghino R, Van Hage-Hamsten M, Virtanen T, Kraft D, Müller MW, Valenta R. I-Microarrayed i-allergenic molecules: abagcini besango lokuxilonga unyango lwe-allergies. FASEB J. 2002 Mar;16(3):414-6. doi: 10.1096/fj.01-0711fje. Epub 2002 Jan 14. PMID: 11790727
- Ferrer M, Sanz ML, Sastre J, Bartra J, del Cuvillo A, Montoro J, Jáuregui I, Dávila I, Mullol J, Valero A. Ukuxilongwa kweemolekyuli kwi-allergology: ukusetyenziswa kwenkqubo ye-microarray. J Phanda iAllergol Clin Immunol. 2009;19 Supply 1:19-24. PMID: 19476050.
- Ott H, Fölster-Holst R, Merk HF, Baron JM. I-Allergen microarrays: isixhobo esinoveli sokulungiswa okuphezulu kwe-IgE kubantu abadala abane-atopic dermatitis. Eur J Dermatol. 2010 Jan-Feb;20(1):54-
61. doi: 10.1684/ejd.2010.0810. Epub 2009 Oct 2. PMID: 19801343. - I-Sastre J. Ukuxilongwa kwe-Molecular kwi-allergies. I-Clin Exp Yokwalana. 2010 Okt;40(10):1442-60. doi: 10.1111/j.1365-2222.2010.03585.x. Epub 2010 Aug 2. PMID: 20682003.
- Martins TB, Bandhauer ME, Bunker AM, Roberts WL, Hill HR. Amathuba amatsha okubhekisa ebuntwaneni nabadala kwi-IgE epheleleyo. J I-Allergy Clin Immunol. 2014 Feb;133(2):589-91.
Ukufumana iinkcukacha malunga nohlalutyo olwenziweyo kunye nezifundo zeklinikhi zibhekiselele kwiimpawu zokusebenza kwi https://www.madx.com/extras.
TSHINTSHA IMBALI
| Inguqulelo | Inkcazo | Uthatha indawo |
| 11 | I-nGal d1 itshintshelwe kwi-rGal d1; URL ihlaziywe ukuya madx.com; I-CE yongezwa ngenani leZiko elaziswayo; utshintsho imbali yongezwa | 10 |
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MacroArray Diagnostics
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MacroArray ALLERGY XPLORER Macro Array Diagnostics [pdf] Imiyalelo 91201229202JQ, 02-2001-01, 02-5001-01, ALLERGY XPLORER Macro Array Diagnostics, ALLERGY XPLORER, Macro Array Diagnostics, Array Diagnostics, Diagnostics |
