MacroArray ALLERGY XPLORER Macro Array Diagnostics

Zofotokozera

• Dzina lazogulitsa: Basic UDI-DI 91201229202JQ
• Nambala Zautumiki: REF 02-2001-01, 02-5001-01
• Kugwiritsa Ntchito Koyenera: Kuzindikira allergen-specific IgE (sIgE) mochulukira komanso IgE yonse (tIgE) semi-quantitatively
• Ogwiritsa: Ogwira ntchito zama labotale ophunzitsidwa bwino komanso akatswiri azachipatala mu labotale yachipatala
• Kusungirako: Ma reagents a Kit amakhala okhazikika kwa miyezi 6 atatsegulidwa

Malangizo Ogwiritsira Ntchito Zogulitsa

Mfundo ya Ndondomeko
Chogulitsacho chimazindikira ma allergen-IgE mochulukira komanso kuchuluka kwa IgE mochulukira.

Kutumiza ndi Kusunga
Onetsetsani kuti zopangira zida zasungidwa monga momwe zasonyezedwera ndipo zimagwiritsidwa ntchito mkati mwa miyezi 6 mutatsegula.

Kutaya Zinyalala:
Tsatirani ndondomeko zoyenera zotayira zinyalala malinga ndi malamulo.

Zida Zamagetsi
Onani buku la ogwiritsa ntchito kuti mumve zambiri za zida.

Zida Zofunika

Kusanthula pamanja: Onetsetsani kuti muli ndi zida zofunika zoperekedwa ndi wopanga.

Kusanthula Mwadzidzidzi: Gwiritsani ntchito chipangizo cha MAX, Washing Solution, Stop Solution, RAPTOR SERVER Analysis Software, ndi PC/Laptop. Tsatirani malangizo okonza mosamala.

Kusamalira Arrays
Tsatirani malangizo omwe aperekedwa pakusamalira masanjidwe mosamala kuti muwonetsetse zotsatira zolondola.

Machenjezo ndi Kusamala

• Valani zida zodzitetezera zoyenera monga zodzitetezera m'manja ndi maso ndi malaya a labu.
• Gwirani ma reagents ndi samposatsata machitidwe abwino a labotale.
• Chitani zinthu zonse zopezeka ndi anthu ngati zomwe zitha kupatsirana ndipo zigwireni mosamala.

FAQ

• Q: Kodi ma reagents amakhala okhazikika mpaka liti?
  A: Ma reagents a Kit amakhala okhazikika kwa miyezi 6 atatsegulidwa akasungidwa pansi pamikhalidwe yomwe yawonetsedwa.
• Q: Ndani angagwiritse ntchito mankhwalawa?
  A: Izi ndizomwe zimagwiritsidwa ntchito ndi ogwira ntchito zachipatala ophunzitsidwa bwino komanso akatswiri azachipatala mu labotale yachipatala.

www.madx.com
CHIWERENGERO XPLORER (ALEX²) MALANGIZO OGWIRITSA NTCHITO

DESCRIPTION

The Allergy Xplorer (ALEX²) ndi Enzyme-Linked Immunosorbent Assay (ELISA) - yochokera ku invitro diagnostic testes for the quantitative measure of allergen-specific IgE (sIgE).
Malangizo Ogwiritsa Ntchitowa akugwiritsidwa ntchito pazinthu izi:

CHOLINGA CHOFUNIKA

ALEX² Allergy Xplorer ndi zida zoyesera zomwe zimagwiritsidwa ntchito poyesa m'thupi la seramu yamunthu kapena madzi a m'magazi (kupatulapo EDTA-plasma) kuti apereke chidziwitso chothandizira kuzindikira odwala omwe akudwala matenda oyambitsidwa ndi IgE molumikizana ndi zomwe zapezedwa kapena zotsatira zoyezetsa. .
Chipangizo chachipatala cha IVD chimazindikira allergen-specific IgE (sIgE) mochulukira komanso kuchuluka kwa IgE (tIgE) semi-quantitatively. Mankhwalawa amagwiritsidwa ntchito ndi ogwira ntchito ophunzitsidwa bwino a labotale komanso akatswiri azachipatala mu labotale yachipatala.

CHIDULE NDI KUTANTHAUZA MAYESO

Zomwe sazidziwa ndi mtundu wa I hypersensitivity reaction ndipo zimayendetsedwa ndi ma antibodies a gulu la IgE la immunoglobulins. Pambuyo pokhudzana ndi zowawa zinazake, kutulutsidwa kwa histamine ndi IgE-mediated kuchokera ku mast cell ndi basophils kumabweretsa mawonetseredwe azachipatala monga mphumu, matupi a rhino-conjunctivitis, atopic eczema, ndi zizindikiro za m'mimba [1]. Chifukwa chake, njira yodziwitsira mwatsatanetsatane kuzinthu zinazake imathandizira pakuwunika odwala omwe ali ndi matupi awo [2-6]. Palibe choletsa pa chiwerengero cha anthu oyesedwa. Mukapanga zoyesa za IgE, zaka ndi kugonana nthawi zambiri sizimaganiziridwa ngati zinthu zofunika kwambiri chifukwa milingo ya IgE, yomwe imayesedwa pamayeserowa, samasiyana kwambiri kutengera kuchuluka kwa anthu.
Magwero onse akuluakulu amtundu wa I amaphimbidwa ndi ALEX². Mndandanda wathunthu wa ALEX² zotulutsa zotulutsa ndi ma cell atha kupezeka pansi pa malangizowa.

Zofunikira kwa wogwiritsa ntchito!
Kuti mugwiritse ntchito molondola ALEX², ndikofunikira kuti wogwiritsa ntchito awerenge mosamala ndikutsatira malangizowa kuti agwiritse ntchito. Wopanga sakhala ndi mlandu wogwiritsa ntchito makina oyesawa omwe sanafotokozedwe m'chikalatachi kapena zosinthidwa ndi wogwiritsa ntchito mayeso.
Chidziwitso: Zosintha za 02-2001-01 za mayeso a ALEX² (20 Arrays) zimangopangidwa kuti zizigwiritsidwa ntchito pamanja. Kuti mugwiritse ntchito mtundu uwu wa ALEX² wokhala ndi makina a MAX 9k, Washing Solution (REF 00-5003-01) ndi Stop Solution (REF 00-5007-01) ziyenera kuyitanidwa padera. Zina zonse zamalonda zitha kupezeka m'malangizo ofananirako oti mugwiritse ntchito: https://www.madx.com/extras.
Zosiyanasiyana za ALEX² 02-5001-01 (magulu 50) zitha kugwiritsidwa ntchito popanga makina ndi MAX 9k (REF 17-0000-01) komanso chipangizo cha MAX 45k (REF 16-0000-01).

MFUNDO YA NJIRA

ALEX² ndi kuyesa kwa immunoassay kutengera Enzyme-Linked Immunosorbent Assay (ELISA). Zotulutsa za Allergen kapena ma allergener a molekyulu, omwe amaphatikizidwa ndi ma nanoparticles, amayikidwa mwadongosolo pagawo lolimba kupanga gulu lalikulu. Choyamba, ma allergens omwe amamangidwa ndi tinthu timachita ndi IgE yeniyeni yomwe imapezeka m'thupi la wodwalayoample. Pambuyo pa makulitsidwe, IgE yosakhala yeniyeni imatsukidwa. Njirayi imapitilira powonjezera ma enzyme otchedwa anti-human IgE detective antibody omwe amapanga zovuta ndi tinthu tating'ono ta IgE. Pambuyo pa sitepe yachiwiri yotsuka, gawo lapansi limawonjezeredwa lomwe limasandulika kukhala losasungunuka, lakuda ndi enzyme yomangidwa ndi antibody. Pomaliza, zochita za enzyme-gawo lapansi zimayimitsidwa ndikuwonjezera chotchinga chotchinga. Kuchuluka kwa mpweya kumayenderana ndi kuchuluka kwa IgE m'magawo a wodwalayoample. Njira yoyesera labu imatsatiridwa ndikupeza zithunzi ndikusanthula pogwiritsa ntchito makina amanja (ImageXplorer) kapena makina odzichitira okha (MAX 45k kapena MAX 9k). Zotsatira zoyezetsa zimawunikidwa ndi RAPTOR SERVER Analysis Software ndikufotokozedwa m'magawo oyankha a IgE (kUA/l). Zotsatira zonse za IgE zimanenedwanso m'magawo oyankha a IgE (kU/l). RAPTOR SERVER ikupezeka mu mtundu 1, kuti mupeze nambala yonse ya manambala anayi chonde onani RAPTOR SERVER imprint yomwe ikupezeka pa www.raptor-server.com/imprint.

KUTUMA NDI KUSINTHA
Kutumiza kwa ALEX² kumachitika pakatentha kozungulira. Komabe, zida ziyenera kusungidwa nthawi yomweyo pakubereka pa 2-8 ° C. Zosungidwa bwino, ALEX² ndi zigawo zake zitha kugwiritsidwa ntchito mpaka tsiku lotha ntchito.

Ma reagents a Kit amakhala okhazikika kwa miyezi 6 atatsegulidwa (pamalo osungira omwe awonetsedwa).

KUtaya zinyalala
Tayani katiriji ya ALEX² yogwiritsidwa ntchito ndi zida zomwe sizinagwiritsidwe ntchito ndi zinyalala za mankhwala a labotale. Tsatirani malamulo onse adziko, boma, ndi amdera lanu okhudza kutaya.

MALAMULO A ZIZINDIKIRO

ZAMBIRI ZA KIT
Chigawo chilichonse (reagent) chimakhala chokhazikika mpaka tsiku lomwe lalembedwa pagawo lililonse. Sitikulimbikitsidwa kuphatikiza ma reagents ochokera kumagulu osiyanasiyana. Kuti mupeze mndandanda wazotulutsa zotulutsa ndi ma cell osasunthika pa ALEX² array, chonde lemberani. support@madx.com.

ZIDA ZOFUNIKA KUPITIRIRA NDI KUSANGALALA

Kusanthula pamanja

• ImageXplorer
• Arrayholder (posankha)
• Lab Rocker (ngodya yokhota 8 °, liwiro lofunikira 8 rpm)
• Chipinda choyezera (WxDxH - 35x25x2 cm)
• RAPTOR SERVER Analysis Software
• PC/Laputopu

Zida zofunika, zosaperekedwa ndi MADx:

• Demineralized Madzi
• Mapaipi & malangizo (100 µl & 100 - 1000 µl)

Kusanthula Mwadzidzidzi:

• Chipangizo cha MAX (MAX 45k kapena MAX 9k)
• Njira Yochapira (REF 00-5003-01)
• Stop Solution (REF 00-5007-01)
• RAPTOR SERVER Analysis Software
• PC/Laputopu

Ntchito zosamalira molingana ndi malangizo a wopanga.

KUGWIRITSA NTCHITO ARRAYS

Osakhudza gulu pamwamba. Kuwonongeka kulikonse komwe kumachitika chifukwa cha zinthu zosawoneka bwino kapena zakuthwa kumatha kusokoneza kuwerenga kolondola kwazotsatira. Osapeza zithunzi za ALEX² mndandanda usanawume (zouma kutentha firiji).

CHENJEZO NDI CHENJEZO

• Ndikoyenera kuvala zodzitchinjiriza pamanja ndi maso komanso malaya a labu ndikutsata machitidwe abwino a labotale pokonzekera ndi kusamalira ma reagents ndi s.amples.
• Mogwirizana ndi machitidwe abwino a labotale, zinthu zonse zopezeka ndi anthu ziyenera kuganiziridwa kuti zitha kupatsirana ndikusamaliridwa ndi njira zodzitetezera monga momwe wodwala amachitira.amples.
• ALEX² Sample Diluent and Washing Solution ili ndi sodium azide (<0.1%) monga chosungira ndipo iyenera kusamaliridwa mosamala. Tsamba lachitetezo chachitetezo likupezeka mukafunsidwa.
• The (ALEX²) Stop Solution ili ndi Ethylenediaminetetraacetic acid (EDTA) -Solution ndipo iyenera kusamaliridwa mosamala. Tsamba lachitetezo chachitetezo likupezeka mukafunsidwa.
• Kwa in-vitro diagnostic ntchito yokha. Osagwiritsidwa ntchito mkati kapena kunja kwa anthu kapena nyama.
• Ogwira ntchito ophunzitsidwa za labotale okha ndi omwe ayenera kugwiritsa ntchito zidazi.
• Mukafika, yang'anani zigawo za zida zowonongeka. Ngati chimodzi mwazinthuzo chawonongeka (monga mabotolo a buffer), funsani MADx (support@madx.com) kapena wogawa kwanuko. Osagwiritsa ntchito zida zowonongeka, chifukwa kugwiritsa ntchito kwawo kungayambitse kusagwira bwino kwa zida.
• Osagwiritsa ntchito ma reagents kuposa masiku awo otha ntchito.
• Osasakaniza ma reagents amitundu yosiyanasiyana.

NJIRA YA ELISA

Kukonzekera
Kukonzekera kwa sampLes: Seramu kapena plasma (heparin, citrate, palibe EDTA) sampmagazi a capillary kapena venous angagwiritsidwe ntchito. Magazi samples akhoza kusonkhanitsidwa pogwiritsa ntchito ndondomeko zokhazikika. Sitolo sampkutsika kwa 2-8 ° C kwa sabata imodzi. Sungani seramu ndi plasma sampkutsika pa -20 ° C posungira nthawi yayitali. Kutumiza kwa seramu/plasma samppang'ono kutentha kutentha ndi ntchito. Lolani nthawi zonse samples kufika kutentha kwa chipinda musanagwiritse ntchito.
Kukonzekera kwa Washing Solution (yokha ya REF 02-5001-01 ndi REF 00-5003-01 ikagwiritsidwa ntchito ndi chipangizo cha MAX): Thirani zomwe zili mu botolo limodzi la Washing Solution mu chidebe chochapira cha chida. Lembani madzi a demineralized mpaka chizindikiro chofiira ndikusakaniza mosamala chidebecho kangapo popanda kutulutsa thovu. The reagent yotsegulidwa imakhala yokhazikika kwa miyezi 1 pa 6-2 ° C.
Chamber of Incubation: Tsekani chivindikiro cha njira zonse zoyeserera kuti mupewe kutsika kwa chinyezi.

Parameters of Kachitidwe:

• 100µl paampndi + 400 µl ALEX² Sampndi Diluent
• 500 µl ALEX² Kuzindikira Antibody
• 500 µl ALEX² Yankho Lapansi
• 100 µl (ALEX²) Stop Solution
• 4500 µl Kusamba Solution

Nthawi yoyeserera ndi pafupifupi 3 h 30 min (popanda kuyanika kosinthidwa).
Sitikulimbikitsidwa kuyendetsa mayeso ochulukirapo kuposa momwe mungapangire mapaipi mu 8 min. Ma incubations onse amachitidwa pa kutentha kwa 20-26 ° C.

Ma reagents onse ayenera kugwiritsidwa ntchito kutentha (20-26 ° C). Kuyesako sikuyenera kuchitidwa padzuwa.

Konzani makulitsidwe chipinda
Tsegulani chipinda chokulitsira ndikuyika matawulo amapepala pansi. Zilowerereni zopukutira zamapepala ndi madzi opanda demineralized mpaka magawo owuma a mapepala amawonekera.

Sampndi incubation/CCD inhibition
Chotsani nambala yofunikira ya makatiriji a ALEX² ndikuyiyika muzogwirizira. Onjezani 400 μl wa ALEX² SampLe Diluent pa cartridge iliyonse. Onjezani 100 μl wodwala sample ku makatiriji. Onetsetsani kuti zotsatira zake zikufalikira mofanana. Ikani makatiriji mu chipinda chokulitsira chokonzekera ndikuyika chipinda choyamwitsira ndi makatiriji pa labu rocker kuti makatiriji agwedezeke pambali patali la cartridge. Yambitsani kukulitsidwa kwa seramu ndi 8 rpm kwa maola awiri. Tsekani chipinda chokulitsira musanayambe chowombera labu. Pambuyo 2 hours, chotsani samples mu chidebe chosonkhanitsira. Pukutani mosamala madontho a katiriji pogwiritsa ntchito thaulo lapepala.

Pewani kukhudza gulu lalikulu ndi pepala chopukutira! Pewani kunyamula kapena kusokoneza sampzochepa pakati pa ALEX² makatiriji!

Zosankha kapena zabwino Hom s LF (CCD chikhomo): ndi muyezo wa CCD antibody inhibition protocol (monga tafotokozera mu ndime 2: sample makulitsidwe/CCD chopinga) kuletsa kwa CCD ndi 85%. Ngati kuchuluka kwa zoletsa kumafunika, konzani 1 ml sample chubu, onjezani 400 μl ALEX² Sample Diluent ndi 100 μl seramu. Yalirani kwa mphindi 30 (osagwedezeka) ndiyeno pitirizani kuyesa mwachizolowezi.
Zindikirani: Kuletsa kwa CCD kowonjezera nthawi zambiri kumabweretsa chiwopsezo cha chitetezo cha CCD choposa 95%.

1 a. Kusamba I
Onjezani 500 μl Washing Solution pa katiriji iliyonse ndikuyika pa rocker labu (pa 8 rpm) kwa mphindi zisanu. Tsukani Njira Yochapira mu chidebe chosonkhanitsira ndikumenya mwamphamvu makatiriji pa mulu wa mapepala owuma. Pukutani mosamala madontho otsala pamakatiriji pogwiritsa ntchito thaulo lapepala.
Bwerezani izi 2 zina.

Onjezani ma antibody
Onjezani 500 µl ya ALEX² Detection Antibody pa cartridge iliyonse.

Onetsetsani kuti gulu lonselo laphimbidwa ndi yankho la ALEX² Detection Antibody.

Ikani makatiriji mu chipinda chokulitsira pa lab rocker ndikuumirira pa 8 rpm kwa mphindi 30. Tsukani njira ya Detection Antibody mu chidebe chosonkhanitsira ndikudina mwamphamvu makatiriji pa mulu wa mapepala owuma. Pukutani mosamala madontho otsala pamakatiriji pogwiritsa ntchito thaulo lapepala.

2 a. Kuchapa II
Onjezani 500 μl Washing Solution pa katiriji iliyonse ndikuyakirani pa rocker labu pa 8 rpm kwa mphindi zisanu. Tsukani Njira Yotsukira mu chidebe chosonkhanitsira ndikumenya mwamphamvu makatiriji pa mulu wa mapepala owuma. Pukutani mosamala madontho otsala pamakatiriji pogwiritsa ntchito thaulo lapepala.
Bwerezani izi 4 zina.

3+4. Onjezani ALEX² Substrate Solution ndikuyimitsa kachitidwe ka gawo lapansi
Onjezani 500 μl ya ALEX² Substrate Solution pa cartridge iliyonse. Yambitsani chowerengera ndi kudzaza katiriji yoyamba ndikupitiriza ndi kudzaza makatiriji otsala. Onetsetsani kuti gawo lonselo laphimbidwa ndi Substrate Solution ndikuyika magawowo kwa mphindi 8 ndendende osagwedezeka (lab rocker pa 0 rpm komanso yopingasa).
Pambuyo pa mphindi 8 ndendende, onjezani 100 μl ya (ALEX²) Stop Solution pamakatiriji onse, kuyambira ndi cartridge yoyamba kutsimikizira kuti magulu onse amalumikizidwa nthawi imodzi ndi ALEX² Substrate Solution. Samalani mosamala kuti mugawire (ALEX²) Stop Solution mu makatiriji otsatizana, pambuyo poti (ALEX²) Stop Solution itayikidwa pamapaipi onse. Pambuyo pake, tulutsani (ALEX²) Substrate/Stop Solution kuchokera pamakatiriji ndikudina mwamphamvu makatiriji pa mulu wa mapepala owuma. Pukutani mosamala madontho aliwonse otsala pamakatiriji pogwiritsa ntchito thaulo lapepala.

Lab Rocker SAYENERA KUGWIRITSA NTCHITO panthawi ya makulitsidwe a gawo lapansi!

4 a. Kusamba III
Onjezani 500 μl Washing Solution pa katiriji iliyonse ndikuyakirani pa rocker labu pa 8 rpm kwa masekondi 30. Tsukani Njira Yotsukira mu chidebe chosonkhanitsira ndikumenya mwamphamvu makatiriji pa mulu wa mapepala owuma. Pukutani mosamala madontho aliwonse otsala pamakatiriji pogwiritsa ntchito thaulo lapepala.

Kusanthula zithunzi
Mukamaliza kuyesa, pukutani mpweya ndi kutentha kwapakati mpaka zitawuma (zitha kutenga mphindi 45).

Kuyanika kwathunthu ndikofunikira kuti timve zoyeserera. Zigawo zouma zouma zokha zomwe zimapereka chizindikiro choyenera ku chiŵerengero cha phokoso.

Pomaliza, zouma zowuma zimawunikidwa ndi ImageXplorer kapena chipangizo cha MAX ndikuwunikidwa ndi pulogalamu ya RAPTOR SERVER Analysis (onani zambiri mu buku la mapulogalamu a RAPTOR SERVER). Pulogalamu ya RAPTOR SERVER Analysis Software imatsimikiziridwa kokha pamodzi ndi chida cha ImageXplorer ndi zipangizo za MAX, choncho MADx sichitenga udindo uliwonse wa zotsatira, zomwe zapezedwa ndi chipangizo china chojambula zithunzi (monga scanner).

Kuyesa Kuyesa

ALEX² master calibration curve idakhazikitsidwa ndikuyesa koyerekeza ndi kukonzekera kwa seramu ndi IgE yeniyeni motsutsana ndi ma antigen osiyanasiyana omwe amaphimba miyeso yomwe akufuna. Magawo ambiri owerengera amaperekedwa ndi RAPTOR SERVER Analysis Software. Zotsatira za mayeso a ALEX² sigE zimawonetsedwa ngati kUA/l. Zotsatira zonse za IgE ndizochepa pang'onopang'ono ndipo zimawerengedwa kuchokera ku muyeso wotsutsa-IgE wokhala ndi zinthu zambiri zofananira, zomwe zimaperekedwa ndi RAPTOR SERVER Analysis Software ndikusankhidwa molingana ndi ma QR-code enieni.
Ma curve pagawo lililonse amasinthidwa ndi makina oyesera a m'nyumba, motsutsana ndi kukonzekera kwa seramu yoyesedwa pa ImmunoCAP (Thermo Fisher Scientific) ya IgE yeniyeni motsutsana ndi zoletsa zingapo. Zotsatira za ALEX² chifukwa chake zimatsatiridwa mwanjira ina motsutsana ndi kukonzekera kwa WHO 11/234 kwa IgE yonse.
Kusiyanasiyana kwadongosolo kwamasigino pakati pa maere kumasinthidwa ndi ma heterologous calibration motsutsana ndi ma curve a IgE. Chinthu chowongolera chimagwiritsidwa ntchito kuti chisinthire mwadongosolo miyeso yosiyana kwambiri.

Kuyeza Range
IgE yeniyeni: 0.3-50 kUA/l kuchuluka
IgE yonse: 20-2500 kU/l semi-quantitative

KUKHALA KWAKHALIDWE

Kusunga zolemba pa kuyesa kulikonse
Malinga ndi machitidwe abwino a labotale tikulimbikitsidwa kulemba manambala a ma reagents onse omwe amagwiritsidwa ntchito.

Control Zitsanzo
Malingana ndi machitidwe abwino a labotale akulimbikitsidwa kuti kuwongolera khalidwe sampLes akuphatikizidwa mkati mwa nthawi zomwe zafotokozedwa. Makhalidwe otengera ma sera owongolera omwe akupezeka pamalonda atha kuperekedwa ndi MADx mukapempha.

KUSANGALALA KWA DATA

Pakuwunika kwazithunzi zamasanjidwe okonzedwa, ImageXplorer kapena chipangizo cha MAX chiyenera kugwiritsidwa ntchito. Zithunzi za ALEX² zimawunikidwa zokha pogwiritsa ntchito pulogalamu ya RAPTOR SERVER Analysis Software ndipo lipoti limapangidwa kuti lifotokoze mwachidule zotsatira za wogwiritsa ntchito.

ZOTSATIRA
ALEX² ndi kuyesa kwachulukidwe kwa ELISA kwa IgE yeniyeni komanso njira yocheperako pa IgE yonse. Ma antibodies a IgE a allergen amawonetsedwa ngati ma IgE reaction unit (kUA/l), zotsatira za IgE zonse monga kU/l. Mapulogalamu a RAPTOR SERVER Analysis Software amawerengera okha ndi kupereka lipoti zotsatira za sIgE (muchulukidwe) ndi zotsatira za tIgE (zotsatira semi-quantitative).

ZOPEREKA NTCHITO

Chidziwitso chotsimikizika chachipatala chiyenera kupangidwa pamodzi ndi zonse zomwe zilipo ndi akatswiri azachipatala ndipo sizidzatengera zotsatira za njira imodzi yokha yodziwira matenda.
M'madera ena ogwiritsira ntchito (mwachitsanzo, kusagwirizana ndi zakudya), ma antibodies a IgE ozungulira amatha kukhala osadziŵika ngakhale kuti zizindikiro za kusagwirizana ndi zakudya zomwe zimatsutsana ndi zomwe sizingagwirizane nazo zingakhalepo, chifukwa ma antibodies amenewa angakhale enieni a allergens omwe amasinthidwa panthawi ya mafakitale, kuphika kapena kugaya. motero sizipezeka pa chakudya choyambirira chomwe wodwalayo amapimidwa.
Zotsatira zoyipa za chiwembu zimangowonetsa kuchuluka kwa ma antibodies a IgE (mwachitsanzo chifukwa cha kusadziwonetsa kwa nthawi yayitali) ndipo sizimalepheretsa kupezeka kwa matenda oopsa kwambiri ku mbola za tizilombo.
Kwa ana, makamaka mpaka zaka ziwiri, chiwerengero cha TIgE chimakhala chochepa kusiyana ndi achinyamata ndi akuluakulu [2]. Chifukwa chake, tiyembekezere kuti mwa ana ochepera zaka 7, mulingo wa IgE wonse umakhala pansi pa malire omwe adziwika. Izi sizikugwira ntchito pamiyezo ya IgE yeniyeni.

MFUNDO ZOYENERA
Kugwirizana kwapakati pakati pa ma allergen-specific IgE antibody ndi matenda osagwirizana nawo amadziwika bwino ndipo akufotokozedwa bwino m'mabuku [1]. Wodwala aliyense wozindikira amawonetsa IgE profile poyesedwa ndi ALEX². Yankho la IgE ndi sampkuchepera kwa anthu athanzi omwe sali ndi matupi awo sagwirizana ndi 0.3 kUA/l pa zinthu zosagwirizana ndi ma molekyulu amodzi komanso zotulutsa zotulutsa zomwe zimatuluka zikayesedwa ndi ALEX². Malo owonetsera IgE yonse mwa akuluakulu ndi <100 kU/l. Kuchita bwino kwa labotale kumalimbikitsa kuti labotale iliyonse ikhazikitse milingo yakeyake yomwe ikuyembekezeka.

ZINTHU ZOCHITIKA
Makhalidwe ogwirira ntchito komanso Chidule cha Chitetezo ndi Magwiridwe angapezeke pa MADx webtsamba: https://www.madx.com/extras.

CHItsimikizo

Deta ya magwiridwe antchito idapezedwa pogwiritsa ntchito njira yomwe yafotokozedwa mu Malangizo Ogwiritsira Ntchito. Kusintha kulikonse kapena kusinthidwa kwa njirayi kungakhudze zotsatira zake ndipo MacroArray Diagnostics imakana zitsimikizo zonse zomwe zafotokozedwa (kuphatikiza chitsimikizo cha malonda ndi kulimba kuti mugwiritse ntchito) pazochitika zotere. Chifukwa chake, MacroArray Diagnostics ndi omwe amagawa kwawoko sadzakhala ndi mlandu wowononga mwanjira ina kapena zotsatira zake pazochitika zotere.

MAFUPITSO

ALLERGEN LIST ALEX²

Zotsatira za Allergen: Aca m, Aca s, Achd, Ail a, All c, All s, Ama r, Amb a, Ana o, Api m, Art v, Ave s, Ber e, Bos d nyama, Bos d milk, Bro p , Cam d, Can f ♂ mkodzo, Can s, Cap a, Cap h epithelia, Cap h milk, Car c, Car i, Car p, Che a, Che q, Chi spp., Cic a, Cit s, Cla h , Clu h, Cor a pollen, Cuc p, Cup s, Cyn d, Dau c, Dol spp., Equ c mkaka, Equ c nyama, Fag e, Fic b, Fic c, Fra e, Gad m, Gal d nyama , Gal d white, Gal d yolk, Hel a, Hom g, Hor v, Jug r, Jun a, Len c, Lit s, Loc m, Lol spp., Lup a, Mac i, Man i, Mel g, Mor r, Mus a, Myt e, Ori v, Ory nyama, Ory s, Ost e, Ovi a epithelia, Ovi a nyama, Ovi a mkaka, Pan b, Pan m, Pap s, Par j, Pas n, Pec spp. , Pen ch, Per a, Pers a, Pet c, Pha v, Phr c, Pim a, Pis s, Pla l, Pol d, Pop n, Pru av, Pru du, Pyr c, Raj c, Rat n, Rud spp., Sac c, Sal k, Sal s, Sco s, Sec c ufa, Sec c mungu, Ses i, Sin, Sol spp., Sola l, Sol t, Sus d epithel, Sus d nyama, Ten m, Thu a, Tri fo, Tri s, Tyr p, Ulm c, Urt d, Vac m, Ves v, Zea m ufa

Zoyeretsedwa zachilengedwe: nAct d 1, nApi m 1, nAra h 1, nAra 3, nBos d 4, nBos d 5, nBos d 6, nBos d 8, nCan f 3, nCor a 9, nCor a 11, nCup a 1, nCry j 1, nEqu c 3, nFag e 2, nGad m 1, nGad m 2 + 3, nGal d 2, nGal d 3, nGal 4, nGal d 5, nGly m 5, nGly m 6, nJug r 4, nMac i 2S Albumin, nOle e 7 (RUO), nPap s 2S Albumin, nPis v 3, nPla a 2, nTri a aA_TI

Zophatikizanso: rAct d 10, rAct d 2, rAct d 5, rAln g 1, rAln g 4, rAlt a 1, rAlt a 6, rAmb a 1, rAmb a 4, rAna o 2, rAna o 3, rAni Ani s 1, rApi g 3, rApi g 1, rApi g 2, rApi m 6, rAra h 10, rAra h 2, rAra h 6, rAra h 8, rAra h 9, rArg r 15, rArt v1 rAsp f 1, rAsp f 3, rAsp f 1, rAsp f 3, rBer e 4, rBet v 6, rBet v 1, rBet v 1, rBla g 2, rBla g 6, rBla g 1, rBla g 2, 4, rBlo t 5, rBlo t 9, rBlo t 10, rBos d 21, rCan f 5, rCan f 2, rCan f 1, rCan f 2, rCan f Fel d 4 ngati, rCan s 6, rCav p 1, a 3, rCla h 1, rClu h 1, rCor a 8, rCor a 1, rCor a 1.0103, rCor a 1.0401 (RUO), rCor a 8, rCra c 12, , rCuc m 14, rCyp 6, rCyp 2 , rDau c 1, rDer f 1, rDer f 1, rDer p 1, rDer p 2, rDer p 1, rDer p 10, rDer p 11, rDer p 2, rDer p 20, rDer p 21, rDer p 23, c 5, rEqu c 7, rFag s 1, rFel d 4, rFel d 1, rFel d 1, rFel d 2, rFra a 4 + 7, rFra e 1, rGal d 3, rGly d m1, rGly d 1, rGly m 2, rHev b 4, rHev b 8, rHev b 1, rHev b 3, rHev b 5, rHev b 6.02, rHom s LF, rJug r 8, rJug r 11, rJug r 1, 2d r Lep 3 , rLol p 6, rMal d 2, rMal d 1, rMala s 1, rMala s 3, rMala s 11, rMal d 5, rMer a 6, rMes a 2 (RUO), rMus m 1, rOle rOle 1, rOry c 1, rOry c 1, rOry c 9, rPar j 1, rPen m 2, rPen m 3, rPen m 2, rPen m 1, rPer a 2, rPhl p 3, rPhl p 4, rPhl rPhl p 7, rPhl p 1, rPhl p 12, rPho d 2, rPhod s 5.0101, rPis v 6, rPis v 7, rPis v 2 (RUO), rPla a 1, rPla a 1, rPla 2 Pol 4, , rPru p 1, rPru p 3 (RUO), rRaj c Parvalbumin, rSal k 1, rSal s 5, rSco s 3, rSes i 7, rSin a 1, rSola l 1, rSus d 1, rThuri a 1, 1, rTri a 6, rTyr p 1, rVes v 1, rVes v 14, rVit v 19, rXip g 2, rZea m 1

MALONJE

1. Hamilton, RG. (2008). Kuunika kwa anthu matupi awo sagwirizana matenda. Clinical Immunology. 1471-1484. 10.1016/B978-0-323-04404-2.10100-9.
2. Harwanegg C, Laffer S, Hiller R, Mueller MW, Kraft D, Spitzauer S, Valenta R. Microarrayed recombinant allergened kuti azindikire za ziwengo. Clin Exp Allergy. 2003 Jan;33(1):7-13. doi: 10.1046/j.1365-2222.2003.01550.x. PMID: 12534543.
3. Hiller R, Laffer S, Hawanegg C, Huber M, Schmidt WM, Twardosz A, Barletta B, Becker WM, Blaser K, Breiteneder H, Chapman M, Crameri R, Duchêne M, Ferreira F, Fiebig H, Hoffmann-Sommergruber K, King TP, Kleber-Janke T, Kurup VP, Lehrer SB, Lidholm J, Müller U, Pini C, Reese G, Scheiner O, Scheynius A, Shen HD, Spitzauer S, Suck R, Swoboda I, Thomas W, Tinghino R, Van Hage-Hamsten M, Virtanen T, Kraft D, Müller MW, Valenta R. Microarrayed mamolekyu a allergen: alonda a zipata zochizira matenda opatsirana. FASEB J. 2002 Mar;16(3):414-6. doi: 10.1096/fj.01-0711fje. Epub 2002 Jan 14. PMID: 11790727
4. Ferrer M, Sanz ML, Sastre J, Bartra J, del Cuvillo A, Montoro J, Jáuregui I, Dávila I, Mullol J, Valero A. Kuzindikira kwa maselo mu allergology: kugwiritsa ntchito njira ya microarray. J Investig Allergol Clin Immunol. 2009; 19 Zowonjezera 1:19-24. Chithunzi cha PMID: 19476050.
5. Ott H, Fölster-Holst R, Merk HF, Baron JM. Ma Allergen Microarrays: Chida Chatsopano Chofotokozera Zapamwamba za IgE Kwa Akuluakulu Omwe Ali ndi Atopic Dermatitis. Eur J Dermatol. 2010 Jan-Feb;20(1):54-
   61. doi: 10.1684/ejd.2010.0810. Epub 2009 Oct 2. PMID: 19801343.
6. Sastre J. Molecular matenda mu ziwengo. Clin Exp Allergy. 2010 Oct;40(10):1442-60. doi: 10.1111/j.1365-2222.2010.03585.x. Epub 2010 Aug 2. PMID: 20682003.
7. Martins TB, Bandhauer ME, Bunker AM, Roberts WL, Hill HR. Nthawi zatsopano zaubwana ndi achikulire pa IgE yonse. J Allergy Clin Immunol. 2014 Feb; 133 (2): 589-91.

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Zolemba / Zothandizira

MacroArray ALLERGY XPLORER Macro Array Diagnostics [pdf] Malangizo 91201229202JQ, 02-2001-01, 02-5001-01, ALLERGY XPLORER Macro Array Diagnostics, ALLERGY XPLORER, Macro Array Diagnostics, Array Diagnostics, Diagnostics

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