BIOSENSORS Daidaita Maganin Mai Amfani Software

Don ƙayyadaddun jagora akan ƙarfin tashin hankali da daidaitawar bayani na daidaitawa, koma zuwa Tebu 2 da aka bayar a cikin littafin mai amfani. Lura cewa ana iya buƙatar wasu haɓakawa bisa tsarin mutum ɗaya.

FAQs

Tambaya: Ta yaya zan adana helicity?
- A: Ajiye heliXcyto bisa ga bayanin ajiya da aka bayar a cikin jagorar mai amfani. Tabbatar duba ranar karewa akan lakabin kuma amfani dashi kafin karewa.

Tambaya: Za a iya amfani da helicity don dalilai na asibiti?
- A: A'a, heliXcyto don amfanin bincike ne kawai kuma bai kamata a yi amfani da shi ba don bincikar asibiti ko magani.

Mabuɗin Siffofin

Don daidaita siginar kyalli akan Spot 1 da Spot 2 na guntuwar heliXcyto
Yana ba da damar yin magana daidai-lokaci na sigina mai kyalli na ja yayin ma'aunin RT-IC

Mai jituwa tare da duk guntuwar helicity
Maganin daidaitawa (jajayen rini) yana ƙunshe da rini mai matsakaicin hydrophilic tare da cajin gidan yanar gizo mai inganci guda ɗaya.

Bayanin Samfura

Lambar oda: NOR-Ra

Tebur 1. Abubuwan da ke ciki da Bayanin Ajiya

Kayan abu	Cap	Ayyukan maida hankali	Adadin	Adana
Daidaitawa mafita -Ra	Lemu	10 µM	6 x 100 µl	-20 °C

Don bincike kawai amfani.
Wannan samfurin yana da ƙayyadaddun rayuwar shiryayye, da fatan za a duba ranar ƙarewar akan alamar.

Shiri

Yi amfani da wannan maganin daidaita rini na ja don ma'aunin RT-IC a cikin tashar ja (wanda ya dogara da alamar nazari).
Rarraba 10 μM daidaitaccen bayani na hannun jari zuwa maida hankali mai aiki tare da buffer mai gudana.

Matsakaicin maganin al'ada ya kamata yayi daidai da ƙaddamarwar fluorophore a cikin mafi girman ƙaddamarwar nazari don aunawa.

Ana iya ƙididdige wannan ta amfani da ma'auni mai zuwa:

: Ƙaddamar da maganin al'ada a cikin launi da ake so
: Ƙaddamar da rini a cikin alamar bincike mai suna bayani

: Mafi girman taro na nazari wanda ya kamata a auna
: Degree na lakabi (rabin rini zuwa nazari)

Ana iya adana hanyoyin da aka diluted a 2-8 ° C har zuwa kwanaki 7.

Bayanin Aikace-aikace

A cikin ma'auni na RT-IC, siginar kyalli na maganin daidaitawa ya kamata ya kasance a cikin irin wannan kewayon azaman sigina mafi girma da ke fitowa daga ƙididdigar da aka ɗaure (raw data). Cikakkiyar siginar kyalli ya dogara ne akan daidaita daidaituwar daidaituwa da ƙarfin kuzarin da ake amfani da shi a ma'aunin. Dole ne a zaɓi ƙarfin motsa jiki bisa ga sigogi masu zuwa:

a. Fluorophore maida hankali a cikin maganin Analyte:
- Matsakaicin fluorophore ya dogara ne akan ƙaddamarwar nazari da aka yi amfani da shi a cikin ma'auni da kuma matakin lakabi na analyte. Don babban DOL da babban ƙididdigar nazari, ana iya buƙatar rage ƙarfin kuzari.
b. Siginar ɗaure da ake tsammani:
- Maƙasudin da aka bayyana sosai akan tantanin halitta na iya ɗaure ƙarin kwayoyin halitta na alamar nazari. A cikin yanayin maƙasudai da yawa, ana iya sa ran siginar ɗaure mai ƙarfi. Don guje wa rufewar, ana iya la'akari da rage ƙarfin motsa jiki.
c. Nau'in guntu:
- Nau'o'in guntu daban-daban suna da bangon haske daban-daban. Mafi girman tarko da kuma ƙarin tarko akan guntu, mafi girman siginar bango. Don haka, kwakwalwan kwamfuta na L5 na iya buƙatar ƙaramin ƙarfin motsa jiki fiye da amfani da guntuwar M5.

Don wurin farawa na ƙarfin motsa jiki da al'ada. Maganin maida hankali da za a yi amfani da shi a cikin gwajin RT-IC, don Allah koma zuwa Tebur 2. Teburin 2. Dangantakar ƙwayar fluorophore, daidaitawar maganin daidaitawa, da ƙarfin motsa jiki wanda ya dace da guntu na heliXcyto M5.

Analyte dye conc. = Analyte cons x DOL	Ƙarfin tashin hankali	Maganin daidaitawa na daidaitawa	Maganin daidaitawa na Dilution
25 nM	0.5	25 nM	1:400
50 nM	0.3	50 nM	1:200
100 nM	0.2	100 nM	1:100
300 nM	0.1	300 nM	1:33
500 nM	0.08	500 nM	1:20
1 µM	0.05	1 µM	1:10
2.5 µM	0.02	2.5 µM	1:4

Lura: Wannan tebur don jagorar ku ne. Koyaya, siginar ƙarshe da aka rubuta a cikin helicity ya dogara da abubuwa da yawa. Don haka, za a buƙaci wasu haɓakawa ga kowane tsarin.

Tuntuɓar

Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Jamus
Dynamic Biosensors, Inc.

Cibiyar Kasuwanci 300, Suite 1400
Wurin, MA 01801
Amurka

Bayanin oda order@dynamic-biosensors.com.
Goyon bayan sana'a support@dynamic-biosensors.com.
www.dynamic-biosensors.com.

Kayan aiki da kwakwalwan kwamfuta an kera su kuma ana kera su a Jamus.
Dynamic Biosensors, Inc. Duk haƙƙin mallaka.

NO-Ra v1.0
www.dynamic-biosensors.com.
Dynamic Biosensors GmbH & Inc.

NO-Ra v1.0

Takardu / Albarkatu

BIOSENSORS Normalisation Solution Software [pdf] Jagorar mai amfani
NOR-Ra, Software na Daidaita Magani, Software na Magani, Software

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Manual mai amfani

BIOSENSORS Normalisation Solution Software

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