i-BIOSENSORS Normalization Solution Software enamandla
Imininingwane
- Igama Lomkhiqizo: ubuvila
- Uhlobo: Isixazululo se-Normalization (udayi obomvu)
- Okwe: Izilinganiso ze-RT-IC esiteshini esibomvu
- Umkhiqizi: I-Dynamic Biosensors GmbH & Inc.
- Inombolo Ye-oda: NOR-Ra
- Ukucwaninga Sebenzisa Kuphela
- Impilo Yeshelufu: Kulinganiselwe, hlola idethi yokuphelelwa yisikhathi kulebula
Izinto ezithinta amandla e-excitation:
- Ukugxila kwe-fluorophore kusisombululo se-analyte
- Isignali ebophezelayo elindelwe
- Uhlobo lweChip
Ukuze uthole isiqondiso esiqondile mayelana namandla avusa amadlingozi kanye nokugxila kwesisombululo esijwayelekile, bheka Ithebula 2 elinikezwe kubhukwana lomsebenzisi. Qaphela ukuthi ukulungiselelwa okuthile kungase kudingeke ngokusekelwe kumasistimu angawodwana.
Imibuzo Evame Ukubuzwa
- Q: Kufanele ngigcine kanjani i-helicity?
- A: Gcina i-heliXcyto ngokolwazi lwesitoreji olunikezwe kumanuwali yomsebenzisi. Qiniseka ukuthi uhlola idethi yokuphelelwa yisikhathi kulebula futhi uyisebenzise ngaphambi kokuphelelwa yisikhathi.
- Umbuzo: Ingabe i-helicity ingasetshenziselwa izinjongo zomtholampilo?
- A: Cha, i-heliXcyto isetshenziselwa ucwaningo kuphela futhi akufanele isetshenziselwe ukuxilonga imitholampilo noma ukwelashwa.
Izici Eziyinhloko
- Ukuze kujwayeleke amasiginali e-fluorescent ku-Spot 1 kanye ne-Spot 2 ye-chip ye-heliXcyto
- Inika amandla ireferensi yesikhathi sangempela elungile yamasiginali e-fluorescent abomvu phakathi nezilinganiso ze-RT-IC
- Ihambisana nawo wonke ama-chips we-helicity
- Isixazululo se-Normalization (udayi obomvu) siqukethe udayi obomvu one-hydrophilic ngokumaphakathi nenetha elilodwa eliphozithivu.
Incazelo Yomkhiqizo
- Inombolo Ye-oda: NOR-Ra
Ithebula 1. Okuqukethwe kanye Nokwaziswa Kwesitoreji
| Okubalulekile | Cap | Isenzo sokugxilisa ingqondo | Inani | Isitoreji |
| Ukujwayela isisombululo-Ra | iwolintshi | 10µM | 6x100µL | -20 °C |
- Okokusetshenziswa kocwaningo kuphela.
- Lo mkhiqizo unomkhawulo wempilo yeshelufu, sicela ubone idethi yokuphelelwa yisikhathi kulebula.
Ukulungiselela
- Sebenzisa lesi sixazululo sokwenza udayi obomvu ube ojwayelekile ezilinganisweni ze-RT-IC esiteshini esibomvu (kuncike kulebula ye-analyte).
- Nciphisa isixazululo sesitoko esijwayelekile esingu-10 μM ekugxiliseni okusebenzayo ngesigcinalwazi esisebenzayo.
- Ukugxiliswa kwesisombululo sokujwayelekile kufanele kulingane nokugxilisana kwe-fluorophore ekugxilweni okuphezulu kwe-analyte okumele kulinganiswe.
Lokhu kungabalwa kusetshenziswa isibalo esilandelayo:
: Ukugxila kwesixazululo sokujwayelekile kumbala oyifunayo
: Ukugxiliswa kukadayi kusixazululo se-analyte esinelebuli
: Ukugxiliswa okuphezulu kwe-analyte okufanele kulinganiswe
: Izinga lokulebula (isilinganiso sikadayi ukuze sihlaziye)
Izixazululo ezihlanjululwe zingagcinwa ku-2-8 ° C kuze kube yizinsuku ezingu-7.
Inothi Lokusebenza
Esilinganisweni se-RT-IC, isignali ye-fluorescent yesisombululo sokujwayelekile kufanele ibe kububanzi obufanayo nesignali ephakeme kakhulu evela kuhlaziyo oluboshwe (idatha eluhlaza). Isignali ye-fluorescent ephelele incike ekugxiliseni kwesixazululo se-normalization namandla ajabulisayo asetshenziswa esilinganisweni. Amandla e-excitation kufanele akhethwe ngokusekelwe kumapharamitha alandelayo:
- a. Ukugxila kwe-fluorophore kusixazululo se-analyte:
- Ukugxila kwe-fluorophore kuncike ekugxilweni kwe-analyte okusetshenziswe esilinganisweni kanye nezinga lokulebula le-analyte. Ukuze uthole i-DOL ephezulu kanye nokugxilisa kwe-analyte ephezulu, ukwehlisa amandla avusa amadlingozi kungase kudingeke.
- b. Isignali ebophezelayo elindelwe:
- Okuqondisiwe kuvezwe kakhulu kuseli kungahlanganisa ama-molecule engeziwe we-analyte enelebula. Endabeni yezinhloso ezivezwe kakhulu, isignali ebophezelayo enamandla ingalindelwa. Ukuze ugweme ukuvala i-shutter, ukwehlisa amandla okuvusa kungase kucatshangelwe.
- c. Uhlobo lwe-chip:
- Izinhlobo zama-chip ezihlukene zinezizinda ze-fluorescent ezihlukene. Uma izicupho ziba zinkulu kanye nezicupho eziningi ku-chip, kulapho isignali engemuva iphakama. Ngakho-ke, ama-chips e-L5 angase adinge amandla aphansi e-excitation kunasetshenziswa kuma-chips e-M5.
Ukuze uthole isiqalo samandla avusa amadlingozi nokujwayelekile. ukugxiliswa kwesixazululo okuzosetshenziswa ekuhlolweni kwe-RT-IC, sicela ubhekisele kuThebula 2. Ithebula 2. Ubudlelwano bokugxila kwe-fluorophore, ukugxila kwesixazululo esijwayelekile, namandla e-excitation afanele chip ye-heliXcyto M5.
| Hlaziya udayi conc. = i-analyte cons x DOL | Amandla okuvusa | Isixazululo se-Concentration Normalization | Isixazululo se-Dilution Normalization |
| 25 nM | 0.5 | 25 nM | 1:400 |
| 50 nM | 0.3 | 50 nM | 1:200 |
| 100 nM | 0.2 | 100 nM | 1:100 |
| 300 nM | 0.1 | 300 nM | 1:33 |
| 500 nM | 0.08 | 500 nM | 1:20 |
| 1µM | 0.05 | 1µM | 1:10 |
| 2.5µM | 0.02 | 2.5µM | 1:4 |
Qaphela: Leli thebula elokuqondisa kwakho. Kodwa-ke, isignali yokugcina erekhodiwe ku-helicity incike ezintweni eziningi. Ngakho-ke, ukuthuthukiswa okuthile kuzodingeka kusistimu ngayinye.
Oxhumana naye
- I-Dynamic Biosensors GmbH
- I-Perchtinger Str. 8/10
- 81379 eMunich
- EJalimane
- Inkampani engu-Dynamic Biosensors, Inc.
- 300 Trade Center, Suite 1400
- I-Woburn, i-MA 01801
- USA
- Oda Ulwazi order@dynamic-biosensors.com.
- Ukusekela kwezobuchwepheshe support@dynamic-biosensors.com.
- www.dynamic-biosensors.com.
- Izinsimbi nama-chips akhiwe futhi akhiqizwa eJalimane.
- ©2024 Dynamic Biosensors GmbH
- Dynamic Biosensors, Inc. Wonke amalungelo agodliwe.
- NOR-Ra v1.0
- www.dynamic-biosensors.com.
- I-Dynamic Biosensors GmbH & Inc.
- NOR-Ra v1.0
Amadokhumenti / Izinsiza
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i-BIOSENSORS Normalization Solution Software enamandla [pdf] Umhlahlandlela Womsebenzisi I-NOR-Ra, Isofthiwe Yesixazululo Esijwayelekile, Isofthiwe Yesixazululo, Isofthiwe |
