dynamic BIOSENSORS Normalization Solution Software

Zofotokozera

• Dzina lazogulitsa: moyo
• Mtundu: Normalization Solution (utoto wofiira)
• Za: Miyezo ya RT-IC munjira yofiyira
• Wopanga: Malingaliro a kampani Dynamic Biosensors GmbH & Inc.
• Nambala yogulira: NOR-Ra
• Kafukufuku Gwiritsani Ntchito Pokha
• Shelf Life: Zochepa, fufuzani tsiku lotha ntchito pa chizindikirocho

Zomwe zimakhudza mphamvu yachisangalalo:

1. Fluorophore concentration mu analyte solution
2. Chizindikiro chomangirira chomwe chikuyembekezeka
3. Mtundu wa Chip

Kuti mupeze chitsogozo chapadera pa mphamvu yachisangalalo ndi kukhazikika kwa njira yothetsera vutoli, onani Table 2 yoperekedwa m'buku la ogwiritsa ntchito. Zindikirani kuti kukhathamiritsa kwina kungafunike potengera machitidwe awoawo.

FAQs

• Q: Ndiyenera kusunga bwanji helicity?
  • A: Sungani heliXcyto monga momwe mukusungirako zomwe zaperekedwa m'buku la ogwiritsa ntchito. Onetsetsani kuti mwawona tsiku lotha ntchito pa cholemberacho ndikuchigwiritsa ntchito lisanathe.
• Q: Kodi helicity ingagwiritsidwe ntchito pazachipatala?
  • A: Ayi, heliXcyto ndi yogwiritsidwa ntchito pofufuza zokha ndipo sayenera kugwiritsidwa ntchito pofufuza matenda kapena kuchiza.

Zofunika Kwambiri

• Pakukhazikika kwa ma sigino a fulorosenti pa Spot 1 ndi Malo 2 a chip heliXcyto
• Imayatsa kulozera kolondola kwa nthawi yeniyeni ya ma siginecha ofiira a fulorosenti pamiyezo ya RT-IC
• Yogwirizana ndi tchipisi ta helicity
• The Normalization solution (utoto wofiira) uli ndi utoto wofiira wa hydrophilic wokhala ndi mtengo umodzi wokha.

Mafotokozedwe Akatundu

• Nambala yogulira: NOR-Ra

Table 1. Zamkatimu ndi Zambiri Zosungira

Zakuthupi	Kapu	Chochitika chokhazikika	Ndalama	Kusungirako
Kukhazikika yankho-Ra	lalanje	10µm	6x100µL	-20 ° C

• Kugwiritsa ntchito kafukufuku kokha.
• Izi zili ndi nthawi yocheperako, chonde onani tsiku lotha ntchito pa lebulo.

Kukonzekera

• Gwiritsani ntchito njira yosinthira utoto wofiyira pakuyeza kwa RT-IC munjira yofiyira (zodalira label ya analyte).
• Sungunulani 10 μM normalization stock solution kuti ikhale yogwira ntchito ndi buffer yothamanga.
• Kuchuluka kwa yankho la normalization kuyenera pafupifupi kufanana ndi kuchuluka kwa fluorophore pamlingo wapamwamba kwambiri womwe ungayesedwe.

Izi zitha kuwerengedwa pogwiritsa ntchito equation iyi:

• : Kukhazikika kwa njira yokhazikika mumtundu womwe mukufuna
• : Kuchuluka kwa utoto mu njira yolembedwa ya analyte
• : Ma analyte apamwamba kwambiri omwe ayenera kuyeza
• : Digiri ya zilembo (chiwerengero cha utoto kuti aunike)

Mayankho osungunuka amatha kusungidwa pa 2-8 ° C kwa masiku 7.

Chidziwitso cha Ntchito

Mu kuyeza kwa RT-IC, chizindikiro cha fulorosenti cha yankho la normalization chiyenera kukhala chofanana ndi chizindikiro chapamwamba kwambiri chochokera ku bound analyte (raw data). Chizindikiro cha fulorosenti chamtheradi chimadalira ndende ya yankho la normalization ndi mphamvu yosangalatsa yomwe imagwiritsidwa ntchito muyeso. Mphamvu yachisangalalo iyenera kusankhidwa kutengera magawo awa:

• a. Kukhazikika kwa fluorophore mu analyte solution:
  • Kuchuluka kwa fluorophore kumatengera kuchuluka kwa analyte komwe kumagwiritsidwa ntchito muyeso komanso kuchuluka kwa zilembo za analyte. Pazowonjezera za DOL komanso kuchuluka kwa ma analyte, kutsitsa mphamvu yosangalatsa kungakhale kofunikira.
• b. Chizindikiro chomangirira chomwe chikuyembekezeka:
  • Zolinga zofotokozedwa kwambiri paselo zimatha kumangirira mamolekyu ambiri a analyte olembedwa. Pankhani ya zolinga zowonongeka kwambiri, chizindikiro champhamvu chomangirira chikhoza kuyembekezera. Kuti mupewe kutseka kwa shutter, kuchepetsa mphamvu yosangalatsa kungaganizidwe.
• c. Mtundu wa Chip:
  • Mitundu yosiyanasiyana ya chip imakhala ndi maziko osiyanasiyana a fulorosenti. Kukula kwa misampha ndi misampha yambiri pa chip, ndipamwamba chizindikiro chakumbuyo. Chifukwa chake, tchipisi ta L5 chitha kufuna mphamvu yocheperako kuposa momwe amapangira tchipisi cha M5.

Kwa poyambira mphamvu yosangalatsa komanso yokhazikika. Kuyika kwa yankho kuti kugwiritsidwe ntchito poyesera RT-IC, chonde onani Table 2. Table 2. Relation of fluorophore concentration, normalization solution concentration, and excitation power eka heliXcyto M5 chip.

Analyte dye conc. = analyte cons x DOL	Mphamvu yosangalatsa	Concentration Normalization solution	Dilution Normalization solution
25 nM	0.5	25 nM	1:400
50 nM	0.3	50 nM	1:200
100 nM	0.2	100 nM	1:100
300 nM	0.1	300 nM	1:33
500 nM	0.08	500 nM	1:20
1µm	0.05	1µm	1:10
2.5µm	0.02	2.5µm	1:4

Zindikirani: Gome ili ndi lowongolera. Komabe, chizindikiro chomaliza cholembedwa mu helicity chimadalira zinthu zambiri. Chifukwa chake, kukhathamiritsa kwina kudzafunika pa dongosolo lililonse.

Contact

• Malingaliro a kampani Dynamic Biosensors GmbH
• Perchtinger Str. 8/10
• 81379 Munich
• Germany
• Malingaliro a kampani Dynamic Biosensors, Inc.
• 300 Trade Center, Suite 1400
• Woburn, MA 01801
• USA
• Kuitanitsa Zambiri order@dynamic-biosensors.com.
• Othandizira ukadaulo support@dynamic-biosensors.com.
• www.dynamic-biosensors.com.
• Zida ndi tchipisi amapangidwa ndikupangidwa ku Germany.
• ©2024 Dynamic Biosensors GmbH
• Dynamic Biosensors, Inc. Ufulu wonse ndi wotetezedwa.
• NOR-Ra v1.0
• www.dynamic-biosensors.com.
• Malingaliro a kampani Dynamic Biosensors GmbH & Inc.
• NOR-Ra v1.0

Zolemba / Zothandizira

dynamic BIOSENSORS Normalization Solution Software [pdf] Buku Logwiritsa Ntchito NOR-Ra, Normalization Solution Software, Solution Software, Software

Maumboni

• Buku Logwiritsa Ntchito