BIOSENSORS eguqukayo yoLungiso lweSoftwe yeSisombululo
Iinkcukacha
- Igama lemveliso: ubunzima
- Uhlobo: Isisombululo esiqhelekileyo (idayi ebomvu)
- Ye: Imilinganiselo ye-RT-IC kumjelo obomvu
- Umenzi: Dynamic Biosensors GmbH & Inc.
- Inombolo yoku-oda: NOR-Ra
- Uphando Sebenzisa Kuphela
- Beka ubomi kwishelufa: Ilinganiselwe, jonga umhla wokuphelelwa kwileyibhile
Izinto ezichaphazela amandla okuvuselela:
- Ugxininiso lweFluorophore kwisisombululo se-analyte
- Umqondiso wokubophelela olindelekileyo
- Uhlobo lwe-Chip
Ukufumana isikhokelo esithe ngqo kumandla okuvuselela kunye nokugxininiswa kwesisombululo esiqhelekileyo, jonga kwiThebhile 2 enikwe kwincwadi yomsebenzisi. Qaphela ukuba ulungiselelo oluthile lungafuneka ngokusekelwe kwiisistim zomntu ngamnye.
Ii-FAQs
- Umbuzo: Ndingazigcina njani i-helicity?
- A: Gcina i-heliXcyto ngokolwazi lokugcina olunikezelwe kwincwadi yomsebenzisi. Qinisekisa ukujonga umhla wokuphelelwa kwileyibhile kwaye uyisebenzise phambi kokuphelelwa yisikhathi.
- Umbuzo: Ngaba i-helicity ingasetyenziselwa iinjongo zonyango?
- A: Hayi, i-heliXcyto yeyokusetyenziswa kophando kuphela kwaye akufanele isetyenziswe kuxilongo lweklinikhi okanye unyango.
Ezona mpawu
- Ukwenziwa kwesiqhelo kweempawu zefluorescent kwiNdawo yoku-1 kunye neNdawo yesi-2 yetshiphu ye-heliXcyto
- Yenza ireferensi echanekileyo yexesha lokwenyani yeempawu ezibomvu zefluorescent ngexesha lemilinganiselo ye-RT-IC
- Iyahambelana nazo zonke iitshiphusi ze-helicity
- Isisombululo se-Normalization (idayi ebomvu) iqulethe idayi ebomvu ephakathi kwehydrophilic enexabiso elinye elilungileyo.
Ingcaciso yeMveliso
- Inombolo yoku-oda: NOR-Ra
Uluhlu loku-1. Imixholo kunye nolwazi loGcino
| Izinto eziphathekayo | Cap | Inyathelo lokugxila | Isixa | Ugcino |
| Ukwenziwa kwesiqhelo isisombululo-Ra | Orenji | 10µM | 6x 100µL | -20 °C |
- Isetyenziselwa uphando kuphela.
- Le mveliso inobomi obulinganiselweyo beshelufu, nceda ubone umhla wokuphelelwa kwileyibhile.
Ukulungiselela
- Sebenzisa esi sisombululo sedayi ebomvu yokulinganisa imilinganiselo ye-RT-IC kumjelo obomvu (i-analyte label-dependent).
- Nciphisa i-10 μM isisombululo sesitokhwe esiqhelekileyo kugxininiso olusebenzayo kunye nesithinteli esisebenzayo.
- Ukugxininiswa kwesisombululo sesiqhelo kufuneka malunga nokuhambelana nokugxininiswa kwe-fluorophore kugxininiso oluphezulu lwe-analyte oluza kulinganiswa.
Oku kungabalwa kusetyenziswa le nxaki ilandelayo:
: Ukugxininiswa kwesisombululo sesiqhelo kumbala ofunekayo
: Ukugxininiswa kwedayi kwisisombululo esibhaliweyo sohlalutyi
: Uxinzelelo oluphezulu lwe-analyte ekufuneka ilinganiswe
: Iqondo lokulebula (umlinganiselo wedayi ukuhlalutya)
Izisombululo ezixutywe zingagcinwa kwi-2-8 ° C ukuya kwii-7 iintsuku.
Inqaku leSicelo
Kwimilinganiselo ye-RT-IC, isignali ye-fluorescent yesisombululo esiqhelekileyo kufuneka sibe kuluhlu olufanayo njengelona phawu luphezulu oluvela kwi-analyte eboshwe (idatha eluhlaza). Isignali ye-fluorescent epheleleyo ixhomekeke ekugxininiseni isisombululo sesiqhelo kunye namandla okuvuselela asetyenziswe kumlinganiselo. Amandla ovuselelo kufuneka akhethwe ngokusekelwe kwezi parameters zilandelayo:
- a. Ugxininiso lweFluorophore kwisisombululo sohlalutyi:
- Ugxininiso lwe-fluorophore luxhomekeke kugxininiso lwe-analyte olusetyenziswa kumlinganiselo kunye neqondo lokuleyibhile kwe-analyte. Kwi-DOL ephezulu kunye nokugxilwa kwe-analyte ephezulu, ukuthoba amandla okuvuselela kunokufuneka.
- b. Umqondiso wokuzibophelela olindelekileyo:
- Iithagethi ezichazwe kakhulu kwiseli zinokubopha iimolekyuli ezininzi ze-analyte ebhaliweyo. Kwimeko ekujoliswe kuyo ngokugqithiseleyo, umqondiso obophelelayo onamandla unokulindelwa. Ukunqanda ukuvala i-shutter, ukuthoba amandla okuvuselela kunokuqwalaselwa.
- c. Uhlobo lwetshiphu:
- Iindidi zetshiphu ezahlukeneyo zinemvelaphi eyahluka-hlukeneyo ye-fluorescent. Inkulu imigibe kunye nemigibe engaphezulu kwi-chip, iphezulu isignali yangasemva. Ke ngoko, iitshiphusi ze-L5 zinokufuna amandla asezantsi okuvuselela kunokuba asetyenziswe kwiitshiphusi ze-M5.
Kwindawo yokuqala yokuvuselela amandla kunye nesiqhelo. Ugxininiso lwesisombululo esiza kusetyenziswa kuvavanyo lwe-RT-IC, nceda ubhekisele kwiTheyibhile 2. Itheyibhile 2. Ubudlelwane bogxininiso lwe-fluorophore, ugxininiso lwesisombululo esiqhelekileyo, kunye namandla okuvuselela afanele ichip ye-heliXcyto M5.
| Hlalutya idayi conc. = hlalutya iingozi x DOL | Amandla okuvuselela | Isisombululo esiQinisekisayo sokuQinisa | Isisombululo se-Dilution Normalization |
| 25 nM | 0.5 | 25 nM | 1:400 |
| 50 nM | 0.3 | 50 nM | 1:200 |
| 100 nM | 0.2 | 100 nM | 1:100 |
| 300 nM | 0.1 | 300 nM | 1:33 |
| 500 nM | 0.08 | 500 nM | 1:20 |
| 1µM | 0.05 | 1µM | 1:10 |
| 2.5µM | 0.02 | 2.5µM | 1:4 |
Phawula: Le theyibhile yeyesikhokelo sakho. Nangona kunjalo, uphawu lokugqibela olurekhodiweyo kwi-helicity luxhomekeke kwizinto ezininzi. Ke, ulungiselelo oluthile luya kufuneka kwinkqubo nganye.
Qhagamshelana
- Dynamic Biosensors GmbH
- Perchtinger Str. 8/10
- 81379 eMunich
- Jemani
- Dynamic Biosensors, Inc.
- 300 iZiko loRhwebo, iSuite 1400
- IWoburn, MA 01801
- i-USA
- Ulwazi lweodolo order@dynamic-biosensors.com.
- Uxhaso lobuchwepheshe support@dynamic-biosensors.com.
- www.dynamic-biosensors.com.
- Izixhobo kunye neetshiphusi zenziwe ngobunjineli kwaye zenziwe eJamani.
- ©2024 Dynamic Biosensors GmbH
- Dynamic Biosensors, Inc. Onke amalungelo agciniwe.
- NOR-Ra v1.0
- www.dynamic-biosensors.com.
- Dynamic Biosensors GmbH & Inc.
- NOR-Ra v1.0
Amaxwebhu / Izibonelelo
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BIOSENSORS eguqukayo yoLungiso lweSoftwe yeSisombululo [pdf] Isikhokelo somsebenzisi I-NOR-Ra, iSisombululo seSisombululo esiQhelekileyo, iSoftware yeSombululo, iSoftware |
