dynamic BIOSENSORS Normalization Solution Software Guide Guide

No ke alakaʻi kikoʻī e pili ana i ka mana excitation a me ka noʻonoʻo hoʻonā maʻamau, e nānā i ka Papa 2 i hāʻawi ʻia ma ka manual mea hoʻohana. E hoʻomanaʻo e koi ʻia paha kekahi ʻano loiloi ma muli o nā ʻōnaehana pākahi.

FAQs

Nīnau: Pehea wau e mālama ai i ka helicity?
- A: E kūʻai i ka heliXcyto e like me ka ʻike mālama i hāʻawi ʻia ma ka manual mea hoʻohana. E hōʻoia e nānā i ka lā pau ma ka lepili a hoʻohana ma mua o ka pau ʻana.
Nīnau: Hiki ke hoʻohana ʻia ka helicity no nā kumu lapaʻau?
- A: ʻAʻole, heliXcyto no ka noiʻi wale nō a ʻaʻole pono e hoʻohana ʻia no nā diagnostics clinical a i ʻole ka mālama ʻana.

Nā mea nui

No ka hana maʻamau o nā hōʻailona fluorescent ma Spot 1 a me Spot 2 o kahi chip heliXcyto

Hiki ke kuhikuhi pololei i ka manawa maoli o nā hōʻailona fluorescent ʻulaʻula i nā ana RT-IC
Kūpono me nā ʻāpana helicity āpau
Aia i loko o ka ho'onā Normalization (ula'ula'ula) ka wai 'ula'ula hydrophilic me ka uku upena maika'i ho'okahi.

Hōʻike huahana

Ka helu kauoha: NOR-Ra

Papa 1. Nā ʻike a me ka mālama ʻana

Mea waiwai	Kāpena	Ka hana concentr	Ka nui	Waihona
Hoʻoponopono maʻamau hoʻonā-Ra	Alani	10 µM	6x 100 µL	-20 °C

No ka noiʻi wale nō.
He palena palena ko kēia huahana, e ʻoluʻolu e ʻike i ka lā pau ma ka lepili.

Hoʻomākaukau

E hoʻohana i kēia ʻano hoʻonā ʻulaʻula maʻamau no nā ana RT-IC ma ke kahawai ʻulaʻula (pili i ka lepili analyte).

E hoʻoheheʻe i ka 10 μM normalization stock solution i kahi ʻano hana me kahi pale holo.
Pono e pili ana ka neʻe ʻana o ka hoʻonā maʻamau me ka ʻike fluorophore i ka ʻike analyte kiʻekiʻe loa e ana ʻia.

Hiki ke helu ʻia kēia me ka hoʻohana ʻana i ka hoohalike penei:

: Ka hoʻopaʻa ʻana o ka hopena maʻamau i ke kala i makemake ʻia
: Ka hoʻopaʻa ʻana o ka wai kala i loko o ka hoʻonā analyte i hōʻailona ʻia
: Kiekie loa o ka analyte pono e ana
: Degere o ka hōʻailona ʻana (ka ratio o ka wai i ka analyte)

Hiki ke mālama ʻia nā hopena i hoʻoheheʻe ʻia ma 2-8 ° C a hiki i 7 mau lā.

Palapala Noi

Ma ke ana RT-IC, pono e like ka hoailona fluorescent o ka solution normalization e like me ka hoailona kiʻekiʻe loa mai ka analyte paʻa (ʻike maka). ʻO ka hōʻailona fluorescent holoʻokoʻa e hilinaʻi ʻia i ka neʻe ʻana o ka hopena maʻamau a me ka mana excitation i hoʻohana ʻia i ke ana. Pono e koho ʻia ka mana excitation ma muli o nā ʻāpana aʻe:

a. ʻO ka hoʻopaʻa ʻana o ka fluorophore i ka hopena analyte:
- Pili ka fluorophore i ka analyte i hoʻohana ʻia ma ke ana a me ke degere o ka lepili ʻana o ka analyte. No ka DOL kiʻekiʻe a me nā analyte kiʻekiʻe kiʻekiʻe, pono e hoʻohaʻahaʻa i ka mana excitation.
b. Hōʻailona paʻa i manaʻo ʻia:
- Hiki i nā pahuhopu i hōʻike ʻia ma ke kelepona ke hoʻopaʻa i nā molekele hou aʻe o ka analyte i hōʻailona ʻia. I ka hihia o nā pahu hopu nui loa, hiki ke manaʻo ʻia kahi hōʻailona paʻa ikaika. No ka pale ʻana i ka pani ʻana o ka pani, e noʻonoʻo ʻia ka hoʻohaʻahaʻa ʻana i ka mana hoʻoulu.
c. ʻAno kīpē:
- Loaʻa i nā ʻano chip like ʻole ke kāʻei fluorescent. ʻO ka nui o nā pahele a ʻoi aku ka nui o nā pahele ma ka chip, ʻoi aku ka kiʻekiʻe o ka hōʻailona hope. No laila, makemake paha nā ʻāpana L5 i ka mana hoʻohaʻahaʻa haʻahaʻa ma mua o ka hoʻohana ʻana i nā pahu M5.

No kahi hoʻomaka o ka mana excitation a me ka maʻamau. e hoʻohana i ka hoʻonā ʻana i ka hoʻokolohua RT-IC, e ʻoluʻolu e nānā i ka Papa 2. Papa 2. Ka pilina o ka ʻike fluorophore, ka hoʻonā ʻana maʻamau, a me ka mana excitation kūpono no kahi chip heliXcyto M5.

conc. = analyte cons x DOL	Mana hoʻohauʻoli	ʻO ka hoʻonā ʻano maʻamau	Hoʻoheheʻe Normalization
25 nM	0.5	25 nM	1:400
50 nM	0.3	50 nM	1:200
100 nM	0.2	100 nM	1:100
300 nM	0.1	300 nM	1:33
500 nM	0.08	500 nM	1:20
1 µM	0.05	1 µM	1:10
2.5 µM	0.02	2.5 µM	1:4

Nānā: No ke alakaʻi kēia papa ʻaina. Eia naʻe, ʻo ka hōʻailona hope i hoʻopaʻa ʻia i ka helicity e pili ana i nā kumu he nui. No laila, e koi ʻia kekahi optimization no kēlā me kēia ʻōnaehana.

Hoʻopili

Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Kelemānia
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA
ʻIke Kauoha order@dynamic-biosensors.com.
Kākoʻo ʻenehana support@dynamic-biosensors.com.
www.dynamic-biosensors.com.
Hoʻokumu ʻia nā mea hana a me nā chips ma Kelemānia.
Dynamic Biosensors, Inc. Ua mālama ʻia nā kuleana āpau.
NOR-Ra v1.0
www.dynamic-biosensors.com.
Dynamic Biosensors GmbH & Inc.
NOR-Ra v1.0

Palapala / Punawai

dynamic BIOSENSORS Normalization Solution Software [pdf] Ke alakaʻi hoʻohana
NOR-Ra, Pūnaehana Hoʻoponopono Normalization, Pūnaehana Hoʻoponopono, Pūnaehana

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Palapala Hoʻohana

dynamic BIOSENSORS Normalization Solution Software

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