dynamic BIOSENSORS Normalization Solution Software
Specifications
- Khoom npe: kev nyab xeeb
- Hom: Normalization Solution (xim liab)
- Rau: RT-IC ntsuas hauv cov channel liab
- Chaw tsim tshuaj paus: Dynamic Biosensors GmbH & Inc.
- Order Number: NOR-Ra
- Rau Kev Tshawb Fawb Siv Xwb
- Lub neej txee: Txwv tsis pub, tshawb xyuas hnub tas sij hawm ntawm daim ntawv lo
Factors cuam tshuam lub zog excitation:
- Fluorophore concentration nyob rau hauv cov tshuaj analytes
- Cia siab tias khi teeb liab
- Chip hom
Rau cov lus qhia tshwj xeeb ntawm lub zog excitation thiab normalization kua concentration, mus rau Table 2 nyob rau hauv cov neeg siv phau ntawv. Nco ntsoov tias qee qhov kev ua kom zoo dua tuaj yeem xav tau raws li tus kheej lub tshuab.
FAQs
- Q: Kuv yuav ua li cas thiaj li khaws cia siab?
- A: Khaws heliXcyto raws li cov ntaub ntawv khaws cia hauv cov neeg siv phau ntawv. Nco ntsoov xyuas hnub tas sij hawm ntawm daim ntawv lo thiab siv nws ua ntej tas sij hawm.
- Q: Helicity puas tuaj yeem siv rau kev kho mob?
- A: Tsis yog, heliXcyto yog siv rau kev tshawb fawb nkaus xwb thiab yuav tsum tsis txhob siv rau kev kuaj mob lossis kev kho mob.
Cov yam ntxwv tseem ceeb
- Rau qhov normalization ntawm cov teeb liab fluorescent ntawm Spot 1 thiab Spot 2 ntawm heliXcyto nti
- Ua kom raug kev xa mus raws sijhawm ntawm cov teeb liab fluorescent liab thaum ntsuas RT-IC
- Tau tshaj txhua yam helicity chips
- Cov tshuaj Normalization (xim liab) muaj cov xim liab hydrophilic nrog ib qho txiaj ntsig zoo
Product Description
- Order Number: NOR-Ra
Table 1. Cov ntsiab lus thiab cov ntaub ntawv khaws cia
| Khoom siv | Cap | Nco ntsoov ua | Tus nqi | Cia |
| normalization daws-Ra | Txiv kab ntxwv | 10 µM | 6 x 100 l | -20 ° C |
- Rau kev tshawb fawb siv xwb.
- Cov khoom no muaj kev txwv lub neej txee, thov saib hnub tas sij hawm ntawm daim ntawv lo.
Kev npaj
- Siv cov xim liab dye normalization tov rau RT-IC ntsuas hauv cov channel liab (analyte label-dependent).
- Dilute 10 μM normalization Tshuag tov rau kev ua hauj lwm concentration nrog ib tug khiav tsis.
- Qhov concentration ntawm cov tshuaj normalization yuav tsum kwv yees li sib haum mus rau fluorophore concentration nyob rau hauv lub siab tshaj plaws analyte concentration yuav tsum tau ntsuas.
Qhov no tuaj yeem suav nrog kev sib npaug hauv qab no:
: Concentration ntawm normalization daws nyob rau hauv cov xim xav tau
: Concentration of dye in the labeled analyte solution
: Qhov siab tshaj plaws ntawm cov tshuaj ntsuam xyuas uas yuav tsum tau ntsuas
: Degree of labeling ( ratio of dye to analyte )
Cov tshuaj diluted tuaj yeem khaws cia ntawm 2-8 ° C mus txog 7 hnub.
Daim ntawv thov
Hauv kev ntsuas RT-IC, lub teeb liab fluorescent ntawm cov tshuaj normalization yuav tsum nyob rau hauv qhov zoo sib xws raws li cov teeb liab siab tshaj plaws los ntawm kev sib khi ua ke (cov ntaub ntawv nyoos). Qhov tseeb fluorescent teeb liab yog nyob ntawm normalization kua concentration thiab lub zog excitation thov nyob rau hauv qhov ntsuas. Lub zog excitation yuav tsum raug xaiv raws li cov hauv qab no tsis:
- a. Fluorophore concentration hauv kev tshuaj ntsuam xyuas:
- Lub fluorophore concentration nyob ntawm qhov ntsuas qhov ntsuas tau siv hauv kev ntsuas nrog rau qib ntawm kev sau npe ntawm cov tshuaj ntsuam xyuas. Rau siab DOL thiab siab analyte concentrations, txo lub zog excitation yuav tsum tau.
- b. Cia siab rau khi teeb liab:
- Cov hom phiaj qhia siab heev ntawm lub xov tooj ntawm tes tuaj yeem khi ntau cov molecules ntawm cov ntawv sau tshuaj ntsuam xyuas. Nyob rau hauv cov ntaub ntawv ntawm lub hom phiaj siab heev, muaj zog khi teeb liab tuaj yeem xav tau. Txhawm rau kom tsis txhob kaw qhov kaw, txo qis lub zog excitation yuav raug txiav txim siab.
- c. Chip hom:
- Cov chips sib txawv muaj cov keeb kwm fluorescent sib txawv. Qhov loj dua cov ntxiab thiab cov ntxiab ntau dua ntawm cov nti, qhov siab dua cov teeb liab tom qab. Yog li, L5 chips yuav xav tau lub zog qis dua li siv rau M5 chips.
Rau qhov pib ntawm excitation zog thiab cov cai. kev daws teeb meem yuav tsum tau siv nyob rau hauv ib qho kev sim RT-IC, thov mus saib cov lus 2. Table 2. Kev sib txheeb ntawm fluorophore concentration, normalization tov concentration, thiab excitation zog haum rau ib tug heliXcyto M5 nti
| Analyte dye conc. = analyte cons x DOL | Excitation zog | Concentration Normalization daws | Dilution Normalization daws |
| 25 nM | 0.5 | 25 nM | 1:400 ua |
| 50 nM | 0.3 | 50 nM | 1:200 ua |
| 100 nM | 0.2 | 100 nM | 1:100 ua |
| 300 nM | 0.1 | 300 nM | 1:33 ua |
| 500 nM | 0.08 | 500 nM | 1:20 ua |
| 1 µM | 0.05 | 1 µM | 1:10 ua |
| 2.5 µM | 0.02 | 2.5 µM | 1:4 ua |
Nco tseg: Lub rooj no yog rau koj kev taw qhia. Txawm li cas los xij, lub teeb liab kawg uas tau sau tseg hauv qhov helicity nyob ntawm ntau yam. Yog li, qee qhov kev ua kom zoo yuav xav tau rau txhua qhov system.
Hu rau
- Dynamic Biosensors GmbH
- Perchtinger Str. 8/10
- 81379 München ua
- Lub teb chaws Yelemees
- Dynamic Biosensors, Inc.
- 300 Trade Center, Suite 1400
- Woburn, MA 01801
- Teb chaws USA
- Cov ntaub ntawv xaj order@dynamic-biosensors.com.
- Kev pab txhawb nqa support@dynamic-biosensors.com.
- www.dynamic-biosensors.com.
- Cov cuab yeej thiab chips yog engineered thiab tsim nyob rau hauv lub teb chaws Yelemees.
- ©2024 Dynamic Biosensors GmbH
- Dynamic Biosensors, Inc. All rights reserved.
- NOR-Ra v1.0
- www.dynamic-biosensors.com.
- Dynamic Biosensors GmbH & Inc.
- NOR-Ra v1.0
Cov ntaub ntawv / Cov ntaub ntawv
 |
dynamic BIOSENSORS Normalization Solution Software [ua pdf] Cov neeg siv phau ntawv qhia NOR-Ra, Normalization Solution Software, Solution Software, Software |
