dynamic BIOSENSORS Normalization Solution Software User Guide

Kuti uwane nhungamiro yakananga pamusoro pesimba rekusimudzira uye kusimudzira mhinduro, tarisa kune Tafura 2 yakapihwa mubhuku remushandisi. Ziva kuti kumwe optimization kunogona kudikanwa zvichibva pane yega masisitimu.

FAQs

Mubvunzo: Ndinofanira kuchengetedza sei helicity?
- A: Chengetedza heliXcyto maererano neruzivo rwekuchengetera rwunopihwa mubhuku remushandisi. Iva nechokwadi chekutarisa zuva rekupera kweiyo label uye rishandise risati rapera.

Mubvunzo: Ko helicity inogona kushandiswa kune zvinangwa zvekiriniki here?
- A: Kwete, heliXcyto ndeyekutsvagisa kushandiswa chete uye haifanirwe kushandiswa kuongororwa kwekiriniki kana kurapwa.

Key Features

Nekugadziriswa kwemasaini efluorescent paSpot 1 uye Spot 2 yeheliXcyto chip.
Inogonesa chaiyo-chaiyo-nguva referensi yemasiginecha matsvuku efluorescent panguva yekuyerwa kweRT-IC

Inoenderana neese helicity chips
Iyo Normalization solution (dhayi dzvuku) ine dhayi ine mwero hydrophilic dzvuku ine imwechete yakanaka net charge.

Product Description

Nhamba yeOdha: NOR-Ra

Tafura 1. Zviri mukati uye Ruzivo Rwekuchengetedza

Material	Cap	Concentr action	Mari	Storage
Normalization mhinduro-Ra	Orenji	10 µM	6x100 µL	-20 °C

Yekushandisa tsvakurudzo chete.
Chigadzirwa ichi chine hupenyu hushoma hwesherufu, ndapota ona zuva rekupera palabel.

Kugadzirira

Shandisa iyi dhayi dzvuku normalization mhinduro yeRT-IC kuyerwa mugero dzvuku (analyte label-dependent).
Dilute iyo 10 μM normalization stock solution kune yekushanda yakasungwa ine inomhanya buffer.

Iko kusungirirwa kweiyo normalization mhinduro kunofanirwa kuenzana neiyo fluorophore concentration mune yepamusoro analyte concentration yekuyerwa.

Izvi zvinogona kuverengerwa uchishandisa inotevera equation:

: Concentration of the normalization solution muruvara rwaunoda
: Kuiswa kwedhayi mune yakanyorwa analyte mhinduro

: Yakanyanya kuwanda kweanalyte inofanirwa kuyerwa
: Dhigirii rekunyora (chiyero chedhayi kuongorora)

Diluted solutions inogona kuchengetwa pa2-8°C kwemazuva manomwe.

Application Note

Muyero yeRT-IC, chiratidzo chefluorescent chemhinduro yenguva dzose inofanira kunge iri mumutsara wakafanana sechiratidzo chepamusoro chinobva kune yakasungwa analyte (raw data). Mhedziso yefluorescent chiratidzo inotsamira pane yakajairwa mhinduro yekumisikidza uye simba rekukurudzira rinoshandiswa mukuyera. Simba rekusimudzira rinofanirwa kusarudzwa zvichibva pane zvinotevera paramita:

a. Fluorophore concentration mune analyte solution:
- Iyo fluorophore concentration inoenderana neiyo analyte concentration inoshandiswa muyero pamwe neiyo dhigirii rezita reanalyte. Kune yakakwira DOL uye yakakwira analyte kutariswa, kudzikisa simba rekusimudzira kunogona kudikanwa.
b. Zvinotarisirwa chiratidzo chekusunga:
- Zvinangwa zvakanyanya pasero zvinogona kusunga mamwe mamorekuru eiyo yakanyorwa analyte. Panyaya yezvinangwa zvakanyanyisa, chiratidzo chakasimba chinosunga chinogona kutarisirwa. Kuti udzivise kuvhara kwevhavha, kuderedza simba rekukurudzira rinogona kufungwa.
c. Chip type:
- Mhando dzakasiyana dzechips dzine akasiyana fluorescent kumashure. Iyo misungo yakakura uye miteyo yakawanda pane chip, iyo inokwidza chiratidzo chekumashure. Naizvozvo, L5 machipi angangoda yakaderera simba rekuwedzeredza pane rinoshandiswa kune M5 machipi.

Nokuda kwekutanga kwesimba rekusimudzira uye tsika. kugadziriswa kwekugadzirisa kushandiswa mukuedza kweRT-IC, tapota tarisa Tafura 2. Tafura 2. Relation of fluorophore concentration, normalization solution concentration, uye simba rekukurudzira rakakodzera heliXcyto M5 chip.

Analyte dye conc. = analyte cons x DOL	Excitation simba	Concentration Normalization solution	Dilution Normalization solution
25 nM	0.5	25 nM	1:400
50 nM	0.3	50 nM	1:200
100 nM	0.2	100 nM	1:100
300 nM	0.1	300 nM	1:33
500 nM	0.08	500 nM	1:20
1 µM	0.05	1 µM	1:10
2.5 µM	0.02	2.5 µM	1:4

Cherechedza: Tafura iyi ndeyekutungamira kwako. Zvisinei, chiratidzo chekupedzisira chakanyorwa muhelicity chinoenderana nezvinhu zvakawanda. Nekudaro, imwe optimization ichadikanwa kune yega yega system.

Contact

Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Jerimani
Nhoroondo ye Dynamic Biosensors, Inc.

300 Trade Center, Suite 1400
Woburn, MA 01801
USA

Order Information order@dynamic-biosensors.com.
Technical Support support@dynamic-biosensors.com.
www.dynamic-biosensors.com.

Zviridzwa nemachipisi zvinogadzirwa uye zvinogadzirwa muGermany.
Dynamic Biosensors, Inc. Kodzero dzese dzakachengetwa.

NOR-Ra v1.0
www.dynamic-biosensors.com.
Nhoroondo ye Dynamic Biosensors GmbH & Inc.

NOR-Ra v1.0

Zvinyorwa / Zvishandiso

dynamic BIOSENSORS Normalization Solution Software [pdf] Bhuku reMushandisi
NOR-Ra, Normalization Solution Software, Solution Software, Software

References

User Manual

dynamic BIOSENSORS Normalization Solution Software

Zvinotsanangurwa