illumina Local Run Manager CF 139-Variant 2.0 Analysis Module Software

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Local Run Manager CF 139-Variant 2.0 Analysis Module Workflow Guide

Fetileview

Mojule oa tlhahlobo oa Local Run CF 139-Variant 2.0 o sebelisoa le TruSight Cystic Fibrosis 139-Variant Assay. Teko e fumana liphetoho tse 139 tse amanang le bongaka tse bakang lefu la cystic fibrosis le mefuta e fapaneng ea liphatsa tsa lefutso tsa cystic fibrosis transmembrane conductance regulator (CFTR) ho genomic DNA e arohaneng le mehlala ea mali a motho. Mojule oa tlhahlobo o etsa tlhahlobo ea bobeli le ho tlaleha tlhahiso ho tsoa ho tatellano e sebelisang TruSight Cystic Fibrosis. Sheba sephutheloana se kenya TruSight Cystic Fibrosis Kit (Tokomane # 1000000097720).
Mojule oa tlhahlobo o hlahloba libaka tse khutšoane tsa ampDNA e entsoeng, kapa amplicon, bakeng sa mefuta e fapaneng. Tatelano e tsepamisitsoeng ea amplicon li etsa hore ho be le tšireletso e phahameng ea libaka tse itseng ho pholletsa le palo e kholo ea samples.
Mabapi le Tataiso ena
Tataiso ena e fana ka litaelo bakeng sa ho theha li-parameter tsa tatellano le tlhahlobo bakeng sa mojule oa tlhahlobo ea CF 139-Variant 2.0. Tšebeliso ea software e hloka tsebo ea motheo ea tsamaiso ea hona joale ea Windows le web sebatli se thehiloeng ho sebatli. Ho fumana leseli mabapi le dashboard ea Local Run Manager le litlhophiso tsa sistimi, bona Local Run Manager Software Reference Guide bakeng sa MiSeqDx.
(tokomane # 1000000011880).
Kenya Run Information

Beha li-Parameters

1. Kena ho Local Run Manager.
2. Khetha Create Run, ebe u khetha CF 139-Variant 2.0.
3. Kenya lebitso la ho matha le supang ho tloha ho tatellano ho ea ho tlhahlobo.
   Sebelisa litlhaku tsa alphanumeric, libaka, underscores, kapa li-dashes (litlhaku tse 40 kapa ka tlase ho moo).
4. [Ka boikhethelo] Kenya tlhaloso ea ho tsamaisa.
   Sebelisa litlhaku tsa alphanumeric, libaka, underscores, kapa li-dashes (litlhaku tse 150 kapa ka tlase ho moo).
5. Kenya nomoro ea lotho le letsatsi la ho felloa ke nako bakeng sa kit ea prep kit.
   Hlalosa Samples bakeng sa Matha
   Hlalosa samples bakeng sa ho matha u sebelisa e 'ngoe ea likhetho tse latelang.
   •  Kenya samples ka letsoho—Sebelisa tafole e se nang letho skrineng sa Create Run. Tlhahiso sample liliba li totobalitsoe.
   • Kenya samples—Eya ho ya kantle file ka sebopeho se arohaneng sa dikoma (*.csv). Setšoantšo se teng bakeng sa ho khoasolla skrineng sa Create Run.

Kena Samples Ka letsoho

1. Kenya e ikhethang sample lebitso ho Sample Lebitso tšimo.
   Sebelisa litlhaku tsa alphanumeric, dashes, kapa underscores (litlhaku tse 40 kapa ka tlase ho moo).
2. Tobetsa ka ho le letona ebe u khetha taolo e ntle le e mpe samples.
   E le ho boloka ho matha, e tlameha ho ba le bonyane taolo e le 'ngoe e ntle le e mpe.
3.  [Ka boikhethelo] Kenya joalo kaample tlhaloso ho sample Tlhaloso tab.
   Sebelisa litlhaku tsa alphanumeric, dashes, kapa underscores (litlhaku tse 50 kapa ka tlase ho moo).
4.  [Ka boikhethelo] Khetha adaptara ea Index 1 ho tsoa lethathamong le theolelang la Index 1 (i7).
   Mohato ona ke oa boikhethelo hobane li-index tsa i7 le i5 li ikemela ka sebopeho sa kamehla.
5.  [Ka boikhethelo] Khetha adaptara ea Index 2 ho tsoa lethathamong le theolelang la Index 2 (i5).
   Mohato ona ke oa boikhethelo hobane li-index tsa i7 le i5 li ikemela ka sebopeho sa kamehla.
6. Kgetha letshwao la Printa ho hlahisa sebopeho sa poleiti.
7. Kgetha Printa ho printa sebopeho sa poleiti e le sesupo sa ho lokisa dilaeborari.
8. [Ka boikhethelo] Kgetha Export to export sample information file.
9. Kgetha Boloka Matha.
   Haeba ka tlase ho 24sampli se li kentsoe, Insufficient Sampfensetere e tla hlaha. Kgetha Tswelapele ho tswelapele, kapa kgetha Hlakola ho edita samples.

TLHOKOMELO
Ho tsoela pele ka nako e ka tlase ho 24samples ha e netefatsoe ke Illumina. Bona TruSight Cystic Fibrosis Package Insert (Tokomane # 1000000097720) bakeng sa tlhaiso-leseling e batsi.

Fumana Samples
Sample tlhahisoleseding e ka imported ho tloha mefuta e 'meli ea files:

• A sample information file eo pele e neng e rometsoe kantle ho mojulung oa CF 139-Variant 2.0 ho sebelisoa karolo ea Export.
• Setšoantšo file, e ka hlahisoang ka ho khetha Template ho skrine sa Create Run. Setšoantšo file e na le lihlooho tse nepahetseng bakeng sa thomello, tse nang le lintlha tsa setšoantšisi kholomong ka 'ngoe. Sebelisa mohlophisi oa kantle ho etsa template file:
• Eketsa sample tlhahisoleseding bakeng sa se seng le se sengample mathang.
• Hang kaofela sampLe tlhahisoleseding e kentswe, hlakola tlhahisoleseding efe kapa efe e setseng ya setshwaro diseleng tse sa sebediswang.
• Boloka template file.

Ho kenya sample information:

1. Khetha Import Samples, ebe u sheba ho file ebe u e khetha.
2. Kgetha letshwao la Printa ho hlahisa sebopeho sa poleiti.
3. Kgetha Printa ho printa sebopeho sa poleiti e le sesupo sa ho lokisa dilaeborari.
4. [Ka boikhethelo] Kgetha Export to export sample tlhahisoleseling ho motho ea kantle file.
5. Kgetha Boloka Matha.
   Haeba ka tlase ho 24sampli se li kentsoe, Insufficient Sampfensetere e tla hlaha. Kgetha Tswelapele ho tswelapele, kapa kgetha Hlakola ho edita samples.

TLHOKOMELO
Ho tsoela pele ka nako e ka tlase ho 24samples ha e netefatsoe ke Illumina. Bona TruSight Cystic Fibrosis Package Insert (Tokomane # 1000000097720) bakeng sa tlhaiso-leseling e batsi.

Edit a Run

Bakeng sa litaelo tsa ho hlophisa tlhahisoleseling pele o tatellano, sheba Local Run Manager
Software Reference Guide bakeng sa MiSeqDx (tokomane # 1000000011880).
Mekhoa ea Tlhahlobo
Bakeng sa tlhaloso ea mekhoa ea tlhahlobo ea TruSight Cystic Fibrosis, bona sephutheloana se kenya TruSight Cystic Fibrosis Kit (Tokomane # 1000000097720).
View Matha le Liphetho

1. Ho tsoa skrineng se seholo sa Local Run Manager, tobetsa lebitso la ho matha.
2. Ho tloha ho Run Overview tab, review metrics ea ho latellana.
3. [Ka boikhethelo] Khetha setšoantšo sa Copy to Clipboard ho kopitsa tsela ea foldara ea tlhahiso.
4. Kgetha tab ya Sequencing Information ho botjhaview tsamaisa lintlha le lintlha tse ka sebelisoang.
5. Khetha khetho ea Samples le Liphetho tab ya ho view liphetho tsa tlhahlobo.
   Thebo e fana ka e leample lenane le akaretsang sekgahla sa mehala, tshebetso, le lesedi mabapi le ditaolo.
   Haeba tlhahlobo e phetoa, eketsa lenane le theoha la Select Analysis 'me u khethe tlhahlobo e nepahetseng.
6. [Ka boikhethelo] Penya habeli sebakeng sa Maikutlo ho kenya maikutlo mabapi le joalo kaample lethathamong. Tobetsa Boloka Liphetoho.
7. Khetha samplenaneng ho bonts'a lintlha tse fapaneng tse fapaneng. Samptlhahisoleseding e fapaneng e bonts'a ka tlase ho sample list.
   HLOKOMELA Nako le nako ha liphetoho li bolokoa, ho hlaha tlhahlobo file e tla hlahisoa foldareng ea Alignment. Sena file e kenyelletsa nako stamp e kenyelelitsoe ho file lebitso, ka sebopeho sa YYMMDD_HHMMSS.

Ho fumana lintlha tse ling ka Run Overview le li-tabo tsa Tatelano ea Boitsebiso, le mokhoa oa ho etsa tlhahlobo ea morao-rao, bona Tataiso ea Reference Software ea Local Run bakeng sa MiSeqDx (toko #
1000000011880).

Lintlha tse ngata tsa ho latela File
Hang ha tlhahlobo e phethiloe, khetha e 'ngoe ea likhetho tse latelang ho view the LotTracking.txt file.

• Ho tsoa ho Sample le Liphetho tab, tobetsa Export Lot Tracking Data.
• Bula the file ka har'a foldara ea Alignment.
  Tsela e eang ho foldara ea Alignment e bonts'oa ho Samples le Liphetho tab ea tšimong ea "Analysis Folder".
• [Ka boikhethelo] Khetha lets'oao la Copy to Clipboard ho kopitsa tsela ea foldara ea tlhahlobo.

Ha tlhahlobo e phethiloe, ho latela lintlha tsa lotho file e ngoletsoe foldareng ea Tolamiso bakeng sa ho matha. Bakeng sa mohlalaample: MiSeqAnalysis\ \Tsamaiso_N\YMMDD_HHMMSS. N ke nomoro ea tatellano e ntseng e eketseha haeba tlhahlobo e ne e requeued. YYMMDD_HHMMSS ke nakoamp tsa ho matha.

Liphetho Tlaleho

Kamora hore tlhahlobo ea bobeli e phethe, liphetho tsa tlhahlobo li akaretsoa ho Samples le Liphetho tab. Liphetho tsa tlhahlobo (sephetho) file hobane tlhahlobo e boetse e akaretsa sephetho ka mongolo o le mong o arotsoeng file e bitsoang TruSightCF139VariantAssay.txt.
Liphetho liphethong tsa tlhahlobo file kenyelletsa lintlha tse fumanehang ho Sample le Liphetho tab. Khetha e 'ngoe ea likhetho tse latelang ho view liphetho tsa tlhahlobo file TruSightCF139VariantAssay.txt:

• Ho tsoa ho Sample le Liphetho tab, tobetsa Export Data.
• Bula the file ka har'a foldara ea Alignment.
  Tsela e eang ho foldara ea Alignment e bonts'oa tšimong ea Analysis Folder ea Samples le Liphetho tab.

HLOKOMELA
Khetha lets'oao la Copy to Clipboard ho kopitsa tsela ea foldara ea tlhahlobo.
Tlhahlobo files bakeng sa mong le e mong tatellano matha li boloketsoe ka seletsa, ka files bolokoa ho
MiSeqAnalysis\ \Data\Intensities\BaseCalls le MiSeqAnalysis\
Lebitso>\Ho tsamaisana_N\YYMMDD_HHMMSS. N ke nomoro ea tatellano e ntseng e eketseha haeba tlhahlobo e ile ea khutlisoa. YYMMDD_HHMMSS ke nakoamp tsa ho matha.

Sephetho sa Tlhahlobo File Boitsebiso
Liphetho tsa tlhahlobo file TruSightCF139VariantAssay.txt e kenyelletsa likarolo tse tharo: the file hlooho, sample tlhahisoleseding, le sample mefuta e fapaneng ya tlhahisoleseding. The file Header e thathamisitsoe pele, 'me e kenyelletsa lintlha tse akaretsang mabapi le ho matha. Hlokomela hore ho na le e le 'ngoe feela file hlooho ka ho matha. Ho fapana le hoo, sample tlhahisoleseding le samplintlha tse fapaneng li kenyellelitsoe bakeng sa sampe sebelisitsoeng tlhahlobong. Likarolo tsena li kopantsoe hammoho bakeng sa se seng le se sengample.
Tafole e latelang e fana ka tlhaloso ea mola o mong le o mong ho file hlooho, e kenyelletsang lintlha tse mabapi le ho matha:
Lethathamo la 1 File Hlooho (Matha Boitsebiso)

• Teko E hlalosa teko e entsoeng. Run ID Run ID e hlahisitsoeng ke MiSeq Operating Software (MOS) qalong ea tatellano ea tatellano.
• Matha Date Date (YYMMDD) eo ho qalisoa ha tatelano ho MOS.
• Analysis Version Software version e neng e sebelisetsoa ho hlahloba.

Tafole e latelang e fana ka tlhaloso ea mola o mong le o mong ho sample likarolo tsa tlhahisoleseding: Lethathamo la 2 Sample Boitsebiso

• Sample ID The sample lebitso le fanoeng ha ho etsoa ho etsoa, ​​​​ho kopantsoe le ID ea tlhahlobo e fanoeng ke software ea Local Run Manager. Haeba u requeue ho matha, sampke reha karolo ea SampID e lula e tšoana, ha ID ea tlhahlobo e fetoha.
• Sebaka sena se ho *.txt file feela.
  Sample Name The sample lebitso le fanoeng ha ho matha ho ne ho bōptjoa.

Tafole e latelang e fana ka tlhaloso ea kholomo ka 'ngoe ho sample mefuta e fapaneng likarolo: Tafole 3 Sample Variants Information

Nalane ea Phetoho

• Tokomane Letsatsi Tlhaloso ea Phetoho
• Tokomane # 1000000100945 v01 August 2021 Aterese ea Moemeli ea Lumelloang oa EU.
• Tokomane # 1000000100945 v00 December 2019 Tokollo ea pele.

Thuso ea Setsebi

Bakeng sa thuso ea tekheniki, ikopanye le Illumina Technical Support.

• Websebaka: www.illumina.com
• Lengolo-tsoibila: techsupport@illumina.com

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Litokomane / Lisebelisoa

illumina Local Run Manager CF 139-Variant 2.0 Analysis Module Software [pdf] Bukana ea Mosebelisi Local Run Manager CF 139-Variant 2.0 Analysis Module, Software, Local Run Manager CF 139-Variant 2.0 Analysis Module Software

Litšupiso

• Bukana ea Mosebelisi