mai tsauri BIOSENSORS AS-2-Rc Cikakken Tsarin Binciken Laboratory Mai sarrafa kansa

Ƙayyadaddun bayanai

• Sunan samfur: heliX+ ADAPTER STRAND 2 tare da jan rini Rc
• Mai ƙiraAbubuwan da aka bayar na Dynamic Biosensors GmbH & Inc.
• Samfura: AS-2-Rc v5.1
• Mabuɗin fasali:
  • 2 tabo tare da jerin anka guda 2 daban-daban don adireshi na DNA.
• Lambar odaSaukewa: AS-2-RC
• Hankali: 400 nM
• Ajiya: Farar hula, da fatan za a duba ranar ƙarewa a kan lakabin. Kauce wa daskare da yawa na hawan keke ta hanyar cire nanolever.

Umarnin Amfani da samfur

Shiri | CIGABA&GUDU

1. Mix Adaftar Strand 1 - Rc (400 nM) da haɗin haɗin Ligand tare da ligand 1 (500 nM) a 1: 1 rabo (v/v).
2. Mix Adaftar Strand 2 - Rc (400 nM) da haɗin haɗin Ligand tare da ligand 2 (500 nM) a 1: 1 rabo (v/v).
3. Sanya mafita guda biyu na mataki na 1 da 2 daban a dakin da zafin jiki a 600 rpm na minti 30 don tabbatar da cikakkiyar haɓakawa.
4. Mix mafita na mataki 1 da 2 a 1: 1 rabo (v/v). An shirya mafita don aikin biochip.

Mabuɗin Siffofin

• Adaftar strand 2 don aiki na heliX® Adaftar Chip Spot 2.
• Mai jituwa tare da heliX® Adafta Chip.
• Ya haɗa da igiyoyin Adafta don sabuntawa 50.
• Mafi dacewa don MIX&RUN sample shiri.
• Adaftar strand 2 yana ɗaukar rini mai jan ruwa na hydrophobic (Rc) tare da cajin gidan yanar gizo tsaka tsaki.

heliX® Adaftar Chip Overview

2 tabo tare da jerin anka guda 2 daban-daban don adireshi na DNA.

Bayanin Samfura

Oda LambaSaukewa: AS-2-RC

Tebur 1. Abubuwan da ke ciki da Bayanan Ajiye

• Don bincike kawai amfani.
• Wannan samfurin yana da iyakataccen rayuwar shiryayye, da fatan za a duba ranar ƙarewa akan lakabin.
• Don kaucewa yawan daskare hawan keke da fatan za a ba da nanolever

Shiri | CIGABA&GUDU

In-mafi haɗakar adaftar da igiyoyin ligand:

1. Mix Adaftar Strand 1 - Rc (400 nM) da haɗin haɗin Ligand tare da ligand 1 (500 nM) a 1: 1 rabo (v/v).
2. Mix Adaftar Strand 2 - Rc (400 nM) da haɗin haɗin Ligand tare da ligand 2 (500 nM) a 1: 1 rabo (v/v).
3. Haɓaka mafita guda biyu na mataki 1 da 2 daban a RT a 600 rpm na mintuna 30 don tabbatar da cikakkiyar haɓakawa.
4. Mix bayani na mataki 1 da 2 a 1: 1 rabo (v/v). Magani yana shirye don amfani don aikin biochip. Tabbatar da maganin yana da alaƙa da kwanciyar hankali na ƙwayoyin ligand.

Table 2. Ƙarin kayan aiki don aiki na tabo 1 da tabo na 2.

Example
Ƙarar da ake buƙata don ayyuka na 3: 100 μL tare da ƙaddamarwar ƙarshe na 100 nM.

Bayan lokacin shiryawa, haɗa vial 1 da vial 2 don samun 100 μL na maganin DNA da aka shirya don amfani.

Tuntuɓar

Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Jamus

Dynamic Biosensors, Inc.
Cibiyar Ciniki 300, Suite 1400 Woburn, MA 01801 Amurka

• Bayanin oda order@dynamic-biosensors.com
• Goyon bayan sana'a support@dynamic-biosensors.com
• www.dynamic-biosensors.com

Kayan aiki da kwakwalwan kwamfuta an kera su kuma ana kera su a Jamus.

©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Duk haƙƙin mallaka.

1. TE40: 10mM Tris, 40mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
2. Idan furotin bai tsaya tsayin daka ba a cikin PE40 (TE40, HE40), da fatan za a duba dacewar buffer tare da takardar dacewa ta switchSENSE®.

www.dynamic-biosensors.com

FAQ

Tambaya: Menene rayuwar shiryayye na samfurin?
A: Samfurin yana da iyakataccen rayuwar shiryayye, da fatan za a koma zuwa ranar ƙarewar kan lakabin.

Tambaya: Ta yaya zan adana samfurin don kiyaye kwanciyar hankali?
A: Ajiye samfurin tare da farar hula don guje wa fallasa zuwa haske. Guji daskarewa-narkewar zagayowar ta hanyar shayar da nanolever.

Tambaya: Ta yaya zan shirya maganin aikin biochip?
A: Bi matakan shirye-shiryen MIX&RUN da aka bayar a cikin littafin mai amfani don shirya mafita don aikin biochip.

Takardu / Albarkatu

mai tsauri BIOSENSORS AS-2-Rc Cikakken Tsarin Binciken Laboratory Mai sarrafa kansa [pdf] Manual mai amfani AS-2-Rc, AS-2-Rc Cikakkiyar Tsarin Nazarin Lantarki Mai sarrafa kansa, Tsarin Bincike Mai sarrafa kansa, Tsarin Nazari Mai sarrafa kansa, Tsarin Binciken Laboratory, Tsarin Bincike, Tsari

Magana

• Manual mai amfani