dynamic BIOSENSORS AS-2-Rc Fully Automated Laboratory Analysis System Manual

Kopanya Adapter strand 1 - Rc (400 nM) le conjugated Ligand strand le ligand 1 (500 nM) ka karolelano ea 1: 1 (v / v).
Kopanya Adapter strand 2 - Rc (400 nM) le conjugated Ligand strand le ligand 2 (500 nM) ka karolelano ea 1: 1 (v / v).

Eketsa ka thoko litharollo tse peli tsa mohato oa 1 le oa 2 mochesong oa kamore ka 600 rpm bakeng sa metsotso e 30 ho netefatsa ho kopanngoa ho felletseng.
Kopanya tharollo ea mohato oa 1 le oa 2 ka karolelano ea 1:1 (v/v). Tharollo e se e loketse ts'ebetso ea biochip.

Likarolo tsa Bohlokoa

Adapter strand 2 bakeng sa ts'ebetso ea heliX® Adapter Chip Spot 2.
E lumellana le heliX® Adapter Chip.

E kenyelletsa likhoele tsa Adapter bakeng sa lintlafatso tse 50.
E loketse MIX&RUN sample litokisetso.

Adapter strand 2 e na le dae e khubelu ea hydrophobic (Rc) e nang le tefiso e sa nke lehlakore.

heliX® Adapter Chip Overview

Libaka tse 2 tse nang le tatellano e 2 e fapaneng ea li-ankora bakeng sa aterese e kentsoeng ka DNA.

Tlhaloso ea Sehlahisoa

Taelo Nomoro: AS-2-Rc

Lethathamo la 1. Litaba le Boitsebiso ba Polokelo

Bakeng sa tšebeliso ea lipatlisiso feela.
Sehlahisoa sena se na le nako ea nako ea shelofo, ka kopo, sheba letsatsi la ho felloa ke nako ho label.

Ho qoba linako tse ngata tsa ho qhibiliha ha leqhoa ka kopo, aliquot the nanolever

Tokisetso | TŠOAKA&RUTHA

In-solution hybridization ea adaptara le likhoele tsa ligand:

Kopanya Adapter strand 1 - Rc (400 nM) le conjugated Ligand strand le ligand 1 (500 nM) ka karolelano ea 1: 1 (v / v).

Kopanya Adapter strand 2 - Rc (400 nM) le conjugated Ligand strand le ligand 2 (500 nM) ka karolelano ea 1: 1 (v / v).
Eketsa ka thoko litharollo tse peli tsa mohato oa 1 le oa 2 ho RT ka 600 rpm bakeng sa metsotso e 30 ho netefatsa ho kopanngoa ho felletseng.
Kopanya tharollo ea mohato oa 1 le oa 2 ka karolelano ea 1: 1 (v/v). Tharollo e se e loketse ho sebelisoa bakeng sa ts'ebetso ea biochip. Ho tsitsa ha tharollo ho amana le botsitso ba limolek'hule tsa ligand.

Lethathamo la 2. Lintlha tse ling bakeng sa ts'ebetso ea sebaka sa 1 le sebaka sa 2 sa litšupiso.

Example
Bophahamo bo hlokahalang bakeng sa ts'ebetso e 3: 100 μL e nang le khatello ea ho qetela ea 100 nM.

Kamora nako ya ho dudisa, kopanya vial 1 le vial 2 ho fumana 100 μL ya tharollo ya DNA e seng e loketse ho sebediswa.

Ikopanye

Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Jeremane

Litlhaloso tsa likarolo tsa Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400 Woburn, MA 01801 USA

Lintlha tsa Odara order@dynamic-biosensors.com
Tšehetso ea tekheniki support@dynamic-biosensors.com
www.dynamic-biosensors.com

Lisebelisoa le li-chips li entsoe le ho etsoa Jeremane.

TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA

Haeba protheine e sa tsitsa ho PE40 (TE40, HE40), ka kopo, hlahloba ts'ebetso ea buffer le letlapa la ho lumellana ha switchSENSE®.

www.dynamic-biosensors.com

LBH

P: Bophelo ba shelofo ea sehlahisoa ke bofe?
A: Sehlahisoa se na le nako e lekanyelitsoeng ea bophelo, ka kopo sheba letsatsi la ho felloa ke nako ho label.

P: Ke lokela ho boloka sehlahisoa joang ho boloka botsitso?
A: Boloka sehlahisoa ka cap e tšoeu ho qoba ho pepesehela khanya. Qoba potoloho ea ho qhibiliha ka ho qotsa nanolever.

P: Ke lokela ho lokisa tharollo joang bakeng sa ts'ebetso ea biochip?
A: Latela mehato ea boitokiso ea MIX&RUN e fanoeng bukeng ea mosebelisi ho lokisa tharollo bakeng sa ts'ebetso ea biochip.

Litokomane / Lisebelisoa

dynamic BIOSENSORS AS-2-Rc Fully Automated Laboratory Analysis System [pdf] Bukana ea Mosebelisi
AS-2-Rc, AS-2-Rc e Felletseng e Ikemetseng ea Ts'ebetso ea Ts'ebetso ea Laeborari, Sistimi ea Tlhahlobo e Ikemetseng ka ho Fetisisa, Sistimi e Ikemetseng ea Ts'ebetso ea Laeborari, Sistimi ea Tlhahlobo ea Laboratori, Sistimi ea Tlhahlobo, Sisteme

Litšupiso

Bukana ea Mosebelisi

dynamic BIOSENSORS AS-2-Rc Fully Automated Laboratory Analysis System

Litaelo tsa Tšebeliso ea Sehlahisoa