dynamic BIOSENSORS AS-2-Rc Fully Automated Laboratory Analysis System Manual User

Teuina: Pa'epa'e pa'epa'e, fa'amolemole va'ai le aso e muta ai le fa'ailoga. Aloese mai le tele o ta'amilosaga fa'a'aisa-liu e ala i le fa'avasegaina o le nanolever.

Fa'atonuga o le Fa'aaogaina o Mea

Sauniuniga | MIX&RUN

Fa'afefiloi le manoa Adapter 1 – Rc (400 nM) ma le laina Ligand fa'atasi ma le ligand 1 (500 nM) ile 1:1 ratio (v/v).
Fa'afefiloi le manoa Adapter 2 – Rc (400 nM) ma le laina Ligand fa'atasi ma le ligand 2 (500 nM) ile 1:1 ratio (v/v).

Fa'a'ese'ese vaifofo e lua o le Laasaga 1 ma le 2 ile vevela ole potu ile 600 rpm mo le 30 min e fa'amautinoa ai le tu'ufa'atasia atoatoa.
Faafefiloi fofo o le Laasaga 1 ma le 2 i le fua faatatau 1:1 (v/v). Ua sauni le fofo mo le fa'aogaina o le biochip.

Vaega Autu

Feso'ota'i strand 2 mo le fa'atinoina o le heliX® Adapter Chip Spot 2.
E fetaui ma le heliX® Adapter Chip.

E aofia ai fa'amau Feso'ota'i mo 50 toe fa'afouina.
Lelei mo MIX&RUN sample sauniuniga.

Adapter strand 2 o lo'o feavea'i se vali mumu hydrophobic (Rc) fa'atasi ai ma se tau fa'aletonu.

HeliX® Adapter Chip Overview

2 nofoaga faʻatasi ma 2 taula eseese faʻasologa mo le DNA-encoded tuatusi.

Fa'amatalaga o oloa

Poloaiga Numera: AS-2-Rc

Laulau 1. Anotusi ma Fa'amatalaga Teuina

Mo na'o su'esu'ega fa'aoga.
O lenei oloa e fa'atapula'a lona ola, fa'amolemole va'ai le aso e muta ai le fa'ailoga.

Ina ia aloese mai le tele o taamilosaga liusuaisa fa'amolemole aliquot le nanolever

Sauniuniga | MIX&RUN

Fa'ato'a fa'atosinaina o mea fa'apipi'i ma manoa ligand:

Fa'afefiloi le manoa Adapter 1 – Rc (400 nM) ma le laina Ligand fa'atasi ma le ligand 1 (500 nM) ile 1:1 ratio (v/v).

Fa'afefiloi le manoa Adapter 2 – Rc (400 nM) ma le laina Ligand fa'atasi ma le ligand 2 (500 nM) ile 1:1 ratio (v/v).
Fa'a'ese'ese vaifofo e lua o le Laasaga 1 ma le 2 ile RT ile 600 rpm mo le 30 min e fa'amautinoa ai le fa'ato'a uma.
Faafefiloi le vaifofo o le laasaga 1 ma le 2 i le fua faatatau 1:1 (v/v). Ole fofo ua sauni e fa'aoga mo le fa'atinoina ole biochip. O le mautu o le fofo e fesoʻotaʻi ma le mautu o molelaʻau ligand.

Laulau 2. Mea fa'aopoopo mo le fa'atinoina o le vaega 1 ma le fa'asinomaga 2.

Example
Volume manaʻomia mo 3 faʻatinoga: 100 μL faʻatasi ai ma le faʻatonuga mulimuli o le 100 nM.

A maeʻa le taimi faʻafefe, faʻafefiloi le vial 1 ma le vial 2 e maua ai le 100 μL o le DNA ua saunia e faʻaaoga.

Fa'afeso'ota'i

Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Siamani

Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400 Woburn, MA 01801 ISA

Fa'amatalaga oka order@dynamic-biosensors.com
Lagolago Fa'atekinisi support@dynamic-biosensors.com
www.dynamic-biosensors.com

O meafaifa'aili ma meataalo e fa'ainisinia ma gaosia i Siamani.

TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA

Afai e le mautu le polotini i le PE40 (TE40, HE40), fa'amolemole siaki le fetaui lelei ma le laupepa feso'ota'iga switchSENSE®.

www.dynamic-biosensors.com

FAQ

Q: O le a le fata o le ola o le oloa?
A: O le oloa e faʻatapulaʻaina le ola, faʻamolemole vaʻai i le aso e muta ai i luga o le igoa.

Q: E tatau faapefea ona ou teuina le oloa e faatumauina le mautu?
A: Teu le oloa i se pulou paʻepaʻe e aloese ai mai le faʻaalia i le malamalama. Aloese mai ta'amilosaga fa'a'aisa-liu e ala i le fa'a'ese'ese o le nanolever.

F: Fa'afefea ona ou saunia le fofo mo le fa'atinoina o le biochip?
A: Mulimuli i laasaga sauniuni MIX&RUN o loʻo tuʻuina atu i le tusi lesona e saunia ai le fofo mo le faʻaogaina o le biochip.

Pepa / Punaoa

dynamic BIOSENSORS AS-2-Rc Fully Automated Laboratory Analysis System [pdf] Tusi Taiala
AS-2-Rc, AS-2-Rc Fully Automated Laboratory Analysis System, Fully Automated Laboratory Analysis System, Automated Laboratory Analysis System, Laboratory Analysis System, Analysis System, System

Fa'asinomaga

Tusi Taiala

dynamic BIOSENSORS AS-2-Rc Fully Automated Laboratory Analysis System