ìmúdàgba BIOSENSORS AS-2-Rc Ni kikun aládàáṣiṣẹ yàrá Analysis System

Awọn pato

• Orukọ ọja: heliX + ADAPTER STRAND 2 pẹlu pupa dai Rc
• Olupese: Yiyi to Biosensors GmbH & Inc.
• Awoṣe: AS-2-Rc v5.1
• Awọn ẹya pataki:
  • Awọn aaye 2 pẹlu awọn ọna itọsẹ 2 oriṣiriṣi oriṣiriṣi fun adirẹsi DNA ti o ni koodu.
• Nọmba ibere: AS-2-Rc
• Ifojusi: 400 nM
• Ibi ipamọ: Fila funfun, jọwọ wo ọjọ ipari lori aami. Yago fun ọpọlọpọ awọn iyipo di-di-diẹ nipa gbigbe nanolever kuro.

Awọn ilana Lilo ọja

Igbaradi | ADALU&RUN

1. Adapọ Adapter strand 1 – Rc (400 nM) ati okun Ligand conjugated pẹlu ligand 1 (500 nM) ni 1: 1 ratio (v/v).
2. Adapọ Adapter strand 2 – Rc (400 nM) ati okun Ligand conjugated pẹlu ligand 2 (500 nM) ni 1: 1 ratio (v/v).
3. Incubate lọtọ awọn ojutu meji ti igbese 1 ati 2 ni iwọn otutu yara ni 600 rpm fun awọn iṣẹju 30 lati rii daju isọdọkan pipe.
4. Illa awọn ojutu ti igbese 1 ati 2 ni ipin 1: 1 (v / v). Solusan ti šetan fun iṣẹ ṣiṣe biochip.

Key Awọn ẹya ara ẹrọ

• Okun Adapter 2 fun iṣẹ ṣiṣe ti heliX® Adapter Chip Spot 2.
• Ni ibamu pẹlu heliX® Adapter Chip.
• Pẹlu awọn okun Adapter fun awọn isọdọtun 50.
• Apẹrẹ fun MIX&RUN sample igbaradi.
• Okun Adapter 2 gbe awọ pupa hydrophobic kan (Rc) pẹlu idiyele apapọ didoju.

heliX® Adapter Chip Loriview

Awọn aaye 2 pẹlu awọn ọna itọsẹ 2 oriṣiriṣi oriṣiriṣi fun adirẹsi DNA ti o ni koodu.

ọja Apejuwe

Bere fun Nọmba: AS-2-Rc

Table 1. Awọn akoonu ati Ibi Alaye

• Fun iwadi nikan lo.
• Ọja yii ni igbesi aye selifu to lopin, jọwọ wo ọjọ ipari lori aami.
• Lati yago fun ọpọlọpọ awọn iyipo didi didi jọwọ gbe nanolever naa

Igbaradi | ADALU&RUN

Ibarapọ-ojutu ti ohun ti nmu badọgba ati awọn okun ligand:

1. Adapọ Adapter strand 1 – Rc (400 nM) ati okun Ligand conjugated pẹlu ligand 1 (500 nM) ni 1: 1 ratio (v/v).
2. Adapọ Adapter strand 2 – Rc (400 nM) ati okun Ligand conjugated pẹlu ligand 2 (500 nM) ni 1: 1 ratio (v/v).
3. Incubate lọtọ awọn ojutu meji ti igbese 1 ati 2 ni RT ni 600 rpm fun awọn iṣẹju 30 lati rii daju isọdọkan pipe.
4. Illa ojutu ti igbese 1 ati 2 ni ipin 1: 1 (v/v). Solusan ti šetan lati lo fun iṣẹ ṣiṣe biochip. Iduroṣinṣin ti ojutu jẹ ibatan si iduroṣinṣin ti awọn ohun elo ligand.

Tabili 2. Awọn ohun elo afikun fun iṣẹ ṣiṣe ti aaye 1 ati aaye itọkasi 2.

Example
Iwọn didun ti a beere fun awọn iṣẹ-ṣiṣe 3: 100 μL pẹlu ifọkansi ipari ti 100 nM.

Lẹhin akoko abeabo, dapọ vial 1 ati vial 2 lati gba 100 μL ti ojutu DNA ti o ṣetan lati lo.

Olubasọrọ

Ìmúdàgba Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Germany

Ìmúdàgba Biosensors, Inc.
300 Trade Center, gbon 1400 Woburn, MA 01801 USA

• Bere fun Alaye order@dynamic-biosensors.com
• Oluranlowo lati tun nkan se support@dynamic-biosensors.com
• www.dynamic-biosensors.com

Awọn ohun elo ati awọn eerun igi jẹ iṣelọpọ ati iṣelọpọ ni Germany.

©2024 Yiyi Biosensors GmbH | Dynamic Biosensors, Inc. Gbogbo ẹtọ wa ni ipamọ.

1. TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
2. Ti amuaradagba ko ba ni iduroṣinṣin ni PE40 (TE40, HE40), jọwọ ṣayẹwo ibamu ifipamọ pẹlu iwe ibamu switchSENSE®.

www.dynamic-biosensors.com

FAQ

Q: Kini igbesi aye selifu ti ọja naa?
A: Ọja naa ni igbesi aye selifu lopin, jọwọ tọka si ọjọ ipari lori aami naa.

Q: Bawo ni MO ṣe le tọju ọja naa lati ṣetọju iduroṣinṣin?
A: Tọju ọja naa pẹlu fila funfun lati yago fun ifihan si ina. Yago fun awọn iyipo di-di-di-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ni-ara.

Q: Bawo ni MO ṣe le mura ojutu fun iṣẹ ṣiṣe biochip?
A: Tẹle awọn igbesẹ igbaradi MIX&RUN ti a pese ni afọwọṣe olumulo lati mura ojutu fun iṣẹ ṣiṣe biochip.

Awọn iwe aṣẹ / Awọn orisun

ìmúdàgba BIOSENSORS AS-2-Rc Ni kikun aládàáṣiṣẹ yàrá Analysis System [pdf] Afowoyi olumulo AS-2-Rc, AS-2-Rc Eto Iṣayẹwo yàrá adaṣe ni kikun, Eto Iṣayẹwo yàrá adaṣe ni kikun

Awọn itọkasi

• Itọsọna olumulo