dynamic BIOSENSORS AS-2-Rc Sistemụ nyocha nyocha ụlọ nyocha zuru oke

Nkọwapụta

• Aha ngwaahịa: heliX+ ADAPTER STRAND 2 nwere ihe na-acha uhie uhie Rc
• Onye nrụpụtaỌnọdụ: Dynamic Biosensors GmbH & Inc.
• Nlereanya: AS-2-Rc v5.1
• Akụkụ ndị bụ isi:
  • 2 tụrụ nwere usoro arịlịka abụọ dị iche iche maka adreesị nwere DNA.
• Nọmba ịtụ ahịa: AS-2-Rc
• itinye uche: 400 nM
• Nchekwa: Okpu ọcha, biko hụ ụbọchị ngwụcha na label. Zenarị ọtụtụ okirikiri oyiri site n'iwepu nanolever.

Ntuziaka ojiji ngwaahịa

Nkwadebe | Mix&RUN

1. Mix Adapter strand 1 - Rc (400 nM) na eriri Ligand jikọtara ya na ligand 1 (500 nM) na 1: 1 ratio (v / v).
2. Mix Adapter strand 2 - Rc (400 nM) na eriri Ligand jikọtara ya na ligand 2 (500 nM) na 1: 1 ratio (v / v).
3. Tinye ihe ngwọta abụọ nke nzọụkwụ 1 na 2 iche iche na ụlọ okpomọkụ na 600 rpm maka 30 min iji hụ na ngwakọ zuru ezu.
4. Gwakọta ihe ngwọta nke nzọụkwụ 1 na 2 na 1: 1 ratio (v / v). Ngwọta adịla njikere maka ịrụ ọrụ biochip.

Atụmatụ igodo

• eriri nkwụnye 2 maka ịrụ ọrụ nke heliX® Adapter Chip Spot 2.
• Dakọtara na HelX® Adapter Chip.
• Gụnyere eriri nkwụnye maka nrụpụta 50.
• Kwesịrị ekwesị maka MIX&RUN sample nkwadebe.
• Ụdọ nkwụnye 2 na-ebu ihe na-acha uhie uhie hydrophobic (Rc) na ụgwọ net na-anọpụ iche.

HelX® Adapter Chip Overview

2 tụrụ nwere usoro arịlịka abụọ dị iche iche maka adreesị nwere DNA.

Nkọwa ngwaahịa

Nye iwu Nọmba: AS-2-Rc

Isiokwu 1. Ọdịnaya na Ozi Nchekwa

• Maka iji naanị nyocha.
• Ngwaahịa a nwere ndụ nchekwa nwere oke, biko hụ ụbọchị ngwụcha na label.
• Iji zere ọtụtụ okirikiri ifriizi, biko tinye nanolever

Nkwadebe | Mix&RUN

Ngwọta nke ngwakọ nke nkwụnye na eriri ligand:

1. Mix Adapter strand 1 - Rc (400 nM) na eriri Ligand jikọtara ya na ligand 1 (500 nM) na 1: 1 ratio (v / v).
2. Mix Adapter strand 2 - Rc (400 nM) na eriri Ligand jikọtara ya na ligand 2 (500 nM) na 1: 1 ratio (v / v).
3. Tinye ihe ngwọta abụọ nke nzọụkwụ 1 na 2 iche iche na RT na 600 rpm maka 30 min iji hụ na ngwakọ zuru ezu.
4. Ngwakọta ngwọta nke nzọụkwụ 1 na 2 na 1: 1 ratio (v / v). Ngwọta adịla njikere iji maka ịrụ ọrụ biochip. Nkwụsi ike nke ngwọta metụtara nkwụsi ike nke mkpụrụ ndụ ligand.

Tebụl 2. Ihe mgbakwunye maka ịrụ ọrụ nke ntụpọ 1 na ntụpọ ntụaka 2.

Example
Olu achọrọ maka ịrụ ọrụ 3: 100 μL na ntinye ikpeazụ nke 100 nM.

Mgbe oge ntinye, gwakọta vial 1 na vial 2 iji nweta 100 μL nke ngwọta DNA dị njikere iji.

Kpọtụrụ

Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Germany

Ọnọdụ nke Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400 Woburn, MA 01801 USA

• Ozi ịtụ order@dynamic-biosensors.com
• Nkwado ndị teknuzu support@dynamic-biosensors.com
• www.dynamic-biosensors.com

A na-arụ ọrụ na ibe na-arụ ọrụ na Germany.

©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Ikike niile echekwabara.

1. TE40: 10mM Tris, 40mM NaCl, 0.05% Tween20, 50 μM EDTA, 50 μM EGTA
2. Ọ bụrụ na protein anaghị akwụsi ike na PE40 (TE40, HE40), biko lelee ndakọrịta ihe nchekwa na mpempe ndakọrịta switchSENSE®.

www.dynamic-biosensors.com

FAQ

Ajụjụ: Gịnị bụ ndụ shelf nke ngwaahịa?
A: Ngwaahịa a nwere oke nchekwa, biko rụtụ aka na ụbọchị ngwụcha na label.

Ajụjụ: Kedu ka m ga-esi echekwa ngwaahịa ahụ ka ọ kwụsie ike?
A: Chekwaa ngwaahịa ahụ na okpu ọcha iji zere ikpughe na ìhè. Zenarị okirikiri oyiri site n'iwepu nanolever.

Ajụjụ: Kedu ka m ga-esi kwado ihe ngwọta maka ịrụ ọrụ biochip?
A: Soro usoro nkwadebe MIX&RUN enyere na ntuziaka onye ọrụ iji kwado ihe ngwọta maka ịrụ ọrụ biochip.

Akwụkwọ / akụrụngwa

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