dynamic BIOSENSORS AS-2-Rc Fully Automated Laboratory Analysis System Buku Logwiritsa Ntchito

Sakanizani chingwe cha Adapter 1 - Rc (400 nM) ndi chingwe cholumikizira cha Ligand chokhala ndi ligand 1 (500 nM) pa chiŵerengero cha 1: 1 (v/v).
Sakanizani chingwe cha Adapter 2 - Rc (400 nM) ndi chingwe cholumikizira cha Ligand chokhala ndi ligand 2 (500 nM) pa chiŵerengero cha 1: 1 (v/v).

Yalirani padera njira ziwiri za sitepe 1 ndi 2 kutentha kwapakati pa 600 rpm kwa mphindi 30 kuti mutsimikizire kusakanizidwa kwathunthu.
Sakanizani mayankho a gawo 1 ndi 2 pa chiyerekezo cha 1:1 (v/v). Yankho ndi okonzeka biochip functionalization.

Zofunika Kwambiri

Adapter strand 2 yogwiritsira ntchito heliX® Adapter Chip Spot 2.
Yogwirizana ndi heliX® Adapter Chip.

Zimaphatikizapo zingwe za Adapter zosinthika 50.
Zabwino kwa MIX&RUN sampndi kukonzekera.

Adapter strand 2 imanyamula utoto wofiira wa hydrophobic (Rc) wokhala ndi ukonde wosalowerera.

heliX® Adapter Chip Overview

Malo 2 okhala ndi katsatidwe ka 2 kosiyanasiyana ka ma adilesi osungidwa ndi DNA.

Mafotokozedwe Akatundu

Order NambalaChithunzi: AS-2-Rc

Table 1. Zamkatimu ndi Zosungirako

Kugwiritsa ntchito kafukufuku kokha.
Chogulitsachi chili ndi nthawi yocheperako, chonde onani tsiku lotha ntchito pa lebulo.

Kuti mupewe maulendo ambiri oundana amaundana chonde aniquot the nanolever

Kukonzekera | MIX&RUN

In-solution hybridization ya adaputala ndi zingwe za ligand:

Sakanizani chingwe cha Adapter 1 - Rc (400 nM) ndi chingwe cholumikizira cha Ligand chokhala ndi ligand 1 (500 nM) pa chiŵerengero cha 1: 1 (v/v).

Sakanizani chingwe cha Adapter 2 - Rc (400 nM) ndi chingwe cholumikizira cha Ligand chokhala ndi ligand 2 (500 nM) pa chiŵerengero cha 1: 1 (v/v).
Yalirani padera njira ziwiri za sitepe 1 ndi 2 pa RT pa 600 rpm kwa mphindi 30 kuti muwonetsetse kusakanizidwa kwathunthu.
Sakanizani njira ya sitepe 1 ndi 2 pa chiŵerengero cha 1:1 (v/v). Yankho ndi okonzeka ntchito biochip functionalization. Kukhazikika kwa yankho kumakhudzana ndi kukhazikika kwa ma molekyulu a ligand.

Table 2. Zina zowonjezera zogwiritsira ntchito malo 1 ndi malo owonetsera 2.

Example
Voliyumu yofunikira pamachitidwe atatu: 3 μL yokhala ndi ndende yomaliza ya 100 nM.

Pambuyo pa makulitsidwe, sakanizani vial 1 ndi vial 2 kuti mupeze 100 μL ya DNA yankho yokonzeka kugwiritsa ntchito.

Contact

Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Germany

Malingaliro a kampani Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400 Woburn, MA 01801 USA

Kuitanitsa Zambiri order@dynamic-biosensors.com
Othandizira ukadaulo support@dynamic-biosensors.com
www.dynamic-biosensors.com

Zida ndi tchipisi amapangidwa ndikupangidwa ku Germany.

Mtengo wa TE40 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA

Ngati mapuloteniwo sali okhazikika mu PE40 (TE40, HE40), chonde fufuzani kuti buffer ikugwirizana ndi switchSENSE® compatibility sheet.

www.dynamic-biosensors.com

FAQ

Q: Kodi alumali moyo wa mankhwala ndi chiyani?
A: Chogulitsacho chili ndi nthawi yocheperako, chonde onani tsiku lotha ntchito palembalo.

Q: Ndiyenera kusunga bwanji mankhwalawa kuti ndikhalebe okhazikika?
Yankho: Sungani mankhwalawa ndi kapu yoyera kuti mupewe kuwala. Pewani kuzizira kozizira polemba nanolever.

Q: Ndiyenera kukonzekera bwanji yankho la biochip functionalization?
A: Tsatirani njira zokonzekera za MIX&RUN zomwe zaperekedwa m'buku la ogwiritsa ntchito kuti mukonzekere yankho la magwiridwe antchito a biochip.

Zolemba / Zothandizira

BIOSENSORS AS-2-Rc Fully Automated Laboratory Analysis System [pdf] Buku Logwiritsa Ntchito
AS-2-Rc, AS-2-Rc Fully Automated Laboratory Analysis System, Fully Automated Laboratory Analysis System, Automated Laboratory Analysis System, Laboratory Analysis System, Analysis System, System

Maumboni

Buku Logwiritsa Ntchito

BIOSENSORS AS-2-Rc Fully Automated Laboratory Analysis System

Malangizo Ogwiritsira Ntchito Zogulitsa