Introduction
The Bio-Rad CFX Connect™ Real-Time PCR Detection System is essential laboratory equipment for researchers conducting polymerase chain reaction (PCR) for applications such as sequencing, cloning, gene expression studies, and mutagenesis. This system offers excellent thermal performance for two-target multiplex PCR and routine detection of single fluorophore experiments. Its adjustable heated lid accommodates a variety of vessels and sealers, including low-profile and standard-height PCR plates.
This guide provides comprehensive instructions on setting up and using the CFX Connect system for sensitive quantitative PCR.
Getting Started
For detailed information on site requirements, including benchspace, environment, and power specifications, please refer to Chapter 2 of this guide. Proper setup ensures optimal performance and longevity of the instrument.
System Overview
The CFX Connect system comprises a thermal cycler base with a reaction module bay, locking bar, and air intake vents for efficient heating and cooling. The back panel features essential ports including Ethernet, USB Type B for computer connection, USB Type A for data transfer, a serial test port, cooling vents, power input, and fuses.
Support and Resources
For technical assistance, contact the Bio-Rad Technical Support Department. The department is available Monday through Friday, 5:00 AM to 5:00 PM, Pacific time. You can reach them by phone at 1-800-424-6723, option 2, or via email at Support@bio-rad.com (U.S./Canada only). For assistance outside the U.S. and Canada, contact your local technical support office or visit www.bio-rad.com for more information, including technical notes, manuals, and product details.
