Olink Target 48 Test Kit

Litaelo tse khutšoanyane

Ho qhoqhoa

Lokisetsa motsoako oa Incubation ka har'a tube ea microcentrifuge ho latela tafole e ka tlase.

Motsoako oa ho elisa ka poleiti ea seliba sa ½ 96 (μL)

• Olink® 1-48 plex Incubation Solution 168
• Olink® Target 48 Frw-probes 21
• Olink® Target 48 Rev-probes 21
• Kakaretso 210

1. Vortex mme o otlolle motsoako oa Incubation. Fetisetsa 23 μL ea motsoako oa Incubation selibeng se seng le se seng sa mochophoro o mocha oa liliba tse 8.
2. Fetisetsa 3 μL ea Motsoako oa ho Incubation selibeng se seng le se seng sa litšiea tse 6 tsa pele tsa poleiti ea liliba tse 96 ka ho chencha liphaephe 'me u rehe poleiti ea Ho Incubation.
3. Kenya 1 μL ea lets e 'ngoe le e 'ngoeample sebelisa pipette ea likanale tse ngata ho ea tlase selibeng, 1 μl ea Sample Taolo ho liliba tse tharo tse holimo (mosehla), 1 μL ea Taolo e Negative ho liliba tse peli (tse khubelu), le 1 μL ea Li-Calibrators ho ea liliba tse tharo (tse tala), ho ea ka sebopeho sa poleiti.
4. Koahela poleiti ka filimi ea polasetiki e khomarelang, e pota-potile ka 400 - 1000 xg, 1 min mocheso oa kamore. E futhumala bosiu bo le bong ho +4 °C.
5. Qhibiliha Tharollo ea PEA bosiu ka +4 °C, 'me u behe PEA Enhancer mocheso oa kamore bosiu kaofela.

Katoloso

Lokisetsa motsoako oa katoloso ho latela tafole e ka tlase.

Motsoako oa katoloso ka poleiti ea seliba sa ½ 96 (μL)

• Metsi a Hloekileng a Phahameng (+4 °C) 4350
• Olink® 1-48 plex PEA Enhancer 580
• Olink® 1-48 plex PEA Solution 580
• Olink® 1-48 plex PEA Enzyme 58
• Kakaretso ea 5 568

1. Tlisa Plate ea ho Incubation mocheso oa kamore, o pota-potile 400 - 1000 xg bakeng sa 1 min. Preheat mochine oa PCR.
2. Vortex motsoako oa Extension 'me ue tšollele ka har'a letamo la pipette ea multichannel.
3. Qala sebali sa nako bakeng sa metsotso e 5 'me u fetisetsa 96 μL ea Motsoako oa Katoloso likarolong tse ka holimo tsa mabota a seliba sa Plate ea ho Incubation ka ho sebelisa pipetting e khutlelang morao.
4. Koala poleiti ka filimi e ncha ea polasetiki e khomarelang, sebelisa MixMate® ho vortex plate ka 2000 rpm bakeng sa metsotsoana e 30, ho etsa bonnete ba hore liliba tsohle li tsoakane, 'me li bilika fatše.
5. Beha Incubation Plate ka har'a sebaesekele se futhumatsang 'me u qale lenaneo la PEA. (50 °C 20 mets, 95 °C 5 mets (95 °C 30 sec, 54 °C 1 min, 60 °C 1 min) x 17, 10 °C tšoara)

Ho lemoha

1. Lokisetsa le ho qala Olink® 48.48 IFC bakeng sa Protein Expression. Ka bokhutšoanyane, kenya sering e le 'ngoe ea mokelikeli ka har'a accumulator e' ngoe le e 'ngoe ho chip, tlosa filimi e sireletsang ho tloha tlase ho IFC ebe u kenya IFC ho Olink® Signature Q100 ho latela litaelo tse skrineng sa sesebelisoa.
2. Thaw the Primer Plate, vortex 'me u bilise hakhutšoanyane.
3. Lokisetsa motsoako oa ho lemoha ho latela tafole e ka tlase.
   Motsoako oa ho lemoha ka poleiti ea seliba sa ½ 96 (μL)
   1. Olink® 1-48 plex Detection Solution 275.0
   2. Metsi a Hloekileng a Phahameng 116.0
   3. Olink® 1-48 plex Enzyme 3.9
   4. Olink® 1-48 plex PCR Polymerase 1.5
   5. Kakaretso 396.4
4. Fetola motsoako oa Detection 'me u otle hakhutšoane' me u kenye 46 μL ea motsoako selibeng se seng le se seng sa seliba se 8.
5. Fetisetsa 7.2 μL ea Motsoako oa Temoho selibeng se seng le se seng sa kholomo ea 1-6 ka poleiting e ncha e nang le liliba tse 96 ka lipeipi tse khutlisetsang morao, 'me u e rehe S.ample Plate.
6. Tlosa Plate ea ho Incubation ho tloha ho sebaesekele se futhumatsang, sotha litaba ebe u fetisetsa 2.8 μL ho S.ample Plate, ho sebelisa pipetting ea pele.
7. Koahela poleiti ka filimi e khomarelang, vortex le spin ka bobeli ho 400 - 1000 xg, 1 min mocheso oa kamore.
8. Fetisetsa 5 μL ho tsoa selibeng se seng le se seng sa kholomo ea 1-6 ea Primer Plate le 5 μL ho tsoa selibeng se seng le se seng sa kholomo ea 1-6 ea S.ample Plate ka har'a 48.48 IFC ea ho kena ka ho le letšehali le ka ho le letona, ka ho latellana. Sebelisa liphaephe tse khutlisetsang morao 'me u fetole malebela ka mor'a hore ho be le primer kapa sample. Se ke oa siea li-inlets li se na letho.
9. Tlosa li-bubble ebe u kenya IFC ho Olink Signature Q100 'me u latele litaelo tse skrineng sa sesebelisoa.
10. Matha IFC ho Olink Signature Q100.

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© 2023 Olink Proteomics AB. Lihlahisoa le litšebeletso tsa Olink ke tsa Tšebeliso ea Lipatlisiso Feela eseng bakeng sa Tšebeliso ea Mekhoa ea Tlhahlobo. Lintlha tsohle tse tokomaneng ena li ka fetoha ntle le tsebiso. Tokomane ena ha e reretsoe ho fana ka litiisetso, boemeli le/kapa likhothaletso tsa mofuta ofe kapa ofe, ntle le haeba litiisetso tse joalo, boemeli le/kapa likhothaletso li boletsoe ka ho hlaka. Olink ha a nke boikarabello bo hlahang liketsong tsa motho ea lebelletsoeng ho bala ho latela tokomane ena. OLINK le mofuta oa logo ea Olink ke matšoao a ngolisitsoeng, kapa a ntseng a emetse ho ngolisoa, ke Olink Proteomics AB. Matshwao ohle a kgwebo a mokga wa boraro ke thepa ya beng ba ona. Lihlahisoa tsa Olink le mekhoa ea ho hlahloba li koahetsoe ke litokelo tse 'maloa tsa litokelo tsa molao le likopo tsa patent https://www.olink.com/patents/. Olink Proteomics, Dag Hammarskjölds väg 52B , SE-752 37 Uppsala, Sweden 1126, v1.3, 2023-01-18

Litokomane / Lisebelisoa

Olink Target 48 Test Kit [pdf] Litaelo Sepheo sa 48, Teko ea Teko, Sepheo sa 48 Test Kit

Litšupiso

• Bukana ea Mosebelisi