Illumina TruSeq Synthetic Long-Read DNA Library Prep Guide

About This Guide

This document serves as a comprehensive protocol for preparing DNA libraries using the Illumina TruSeq Synthetic Long-Read DNA Library Prep Kit and the TruSeq Synthetic Long-Read DNA Barcode Kit. It is designed to guide researchers through the process of generating high-quality DNA libraries suitable for advanced genomic analyses.

Key Applications Supported

Long-Read Assembly: Facilitates the construction of accurate long contigs for de novo genome assembly, genome finishing, and metagenomics.
Phasing Analysis: Enables the preparation of DNA libraries for phasing analysis, identifying co-inherited alleles and de novo mutations in whole human genomes.

Document Scope

The guide details essential procedures including DNA fragmentation, end repair, adapter ligation, purification, size selection, and quality control. It emphasizes best practices for research use and requires trained personnel for execution.

For further product information, technical support, and resources, visit the official Illumina website:

	Illumina DNA Prep with Enrichment Dx Kit: Targeted DNA Library Preparation The Illumina DNA Prep with Enrichment Dx Kit is a comprehensive solution for preparing DNA sequencing libraries from both peripheral whole blood and formalin-fixed, paraffin-embedded (FFPE) tissue samples. This kit is designed to enrich libraries for specific genomic regions of interest, enabling targeted sequencing applications. It supports DNA from whole blood and FFPE tissues, utilizes user-supplied probe panels for target enrichment, and is compatible with Illumina sequencing systems.
	Illumina Nextera Mate Pair Library Prep Reference Guide A comprehensive reference guide detailing the Illumina Nextera Mate Pair Library Prep protocol for generating mate pair libraries from genomic DNA for paired-end sequencing. Covers gel-free and gel-plus protocols, DNA input recommendations, and technical assistance.
	Illumina TruSeq Bovine Parentage Reference Guide for DNA Sequencing Comprehensive guide to preparing DNA libraries for bovine parentage analysis using the Illumina TruSeq Bovine Parentage Kit, covering protocols, input recommendations, and technical assistance.
	TruSight Cardio Sequencing Kit Reference Guide This guide provides detailed instructions for preparing up to 48 indexed paired-end libraries using the TruSight Cardio Sequencing Kit, including fragmentation, amplification, hybridization, and capture steps for targeted resequencing applications.
	Illumina Local Run Manager Germline Variant Analysis Module Workflow Guide for NextSeq 550Dx This document provides a workflow guide for the Illumina Local Run Manager Germline Variant Analysis Module on the NextSeq 550Dx instrument. It details the process of setting up runs, entering sample information, importing manifests, performing analysis, and viewing results, specifically for germline variant detection in DNA from peripheral whole blood specimens. The guide also covers output file formats, folder structures, base calling, index diversity, revision history, and technical assistance.
	Nextera Mate Pair Sample Preparation Guide - Illumina Comprehensive guide for preparing Nextera Mate Pair DNA libraries for sequencing using Illumina's TruSeq technology. Covers Gel-Free and Gel-Plus protocols, workflow, best practices, and technical assistance.
	Illumina MiSeq System: Denature and Dilute Libraries Guide A comprehensive guide from Illumina detailing the procedures for denaturing and diluting DNA libraries for sequencing on the MiSeq system, including protocols for various normalization methods and PhiX control preparation.
	Illumina DNA Prep with Enrichment Checklist A comprehensive checklist for preparing and enriching DNA libraries using the Illumina DNA Prep with Enrichment kit, detailing steps for tagmentation, cleanup, amplification, pooling, and quality control.

About This Guide

Key Applications Supported

Document Scope

Related Documents