Introduction to the TruSight Cardio Sequencing Kit
This document serves as a comprehensive reference guide for the Illumina TruSight® Cardio Sequencing Kit. It details the protocol for preparing up to 48 indexed paired-end libraries, which are then used for targeted resequencing applications. The kit is designed for efficient and reliable DNA library preparation, offering advantages such as fast sample preparation, low DNA input requirements, and excellent data quality.
The TruSight Cardio protocol involves several key steps, including DNA fragmentation, amplification, hybridization, and capture. Each step is outlined with specific reagents and procedures to ensure optimal results. The guide also provides essential information on DNA input recommendations, tips for avoiding cross-contamination, and handling techniques for beads and plates.
For additional resources and support, including a Custom Protocol Selector and technical notes, please visit the Illumina support website at support.illumina.com.
