Illumina Nextera Mate Pair Library Prep Reference Guide

Overview

This document provides a comprehensive guide for preparing mate pair libraries from genomic DNA using the Illumina Nextera Mate Pair Library Prep Kit. It is designed for researchers and laboratory professionals involved in next-generation sequencing workflows.

The Nextera Mate Pair protocol facilitates the generation of libraries suitable for paired-end sequencing, offering flexibility with both gel-free and optional gel-plus protocols. Key features include efficient DNA fragmentation and adapter tagging using transposomes, on-bead reactions for automation, and compatibility with TruSeq DNA Library Prep adapter indexing.

Key Features and Protocols:

DNA fragmentation and adapter tagging
Gel-free and Gel-plus protocol options
DNA input recommendations and quality assessment
Detailed step-by-step procedures for library preparation
Guidance on size selection, circularization, and amplification
Information on consumables, equipment, and technical assistance

For customized workflow documentation and further resources, visit the Illumina support website:

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Overview

Key Features and Protocols:

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