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Illumina MiSeq System: Denature and Dilute Libraries Guide

This guide provides essential instructions for preparing DNA libraries for sequencing on the Illumina® MiSeq® system. It details the critical steps involved in denaturing and diluting prepared libraries to achieve optimal results.

Key procedures covered include:

Standard Normalization Method (Protocol A)
Bead-Based Normalization Method (Protocol B)
AmpliSeq™ for Illumina Panels Normalization Method (Protocol C)
AmpliSeq Library Equalizer™ for Illumina Normalization Method (Protocol D)

Additionally, the guide offers instructions for preparing a PhiX library, which serves as a crucial sequencing control, especially for low-diversity samples. It also outlines best practices and provides supplemental information for users.

For further details on product usage, legal information, and support, please visit the Illumina website.

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