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Illumina Nextera Mate Pair Sample Preparation Guide

This guide provides detailed instructions for preparing Nextera Mate Pair DNA libraries for paired-end sequencing using Illumina's advanced technology.

It outlines two distinct protocols:

Gel-Free Protocol: A streamlined, low-input method for generating libraries with a broad fragment size distribution.
Gel-Plus Protocol: Offers more stringent size selection for applications requiring specific fragment sizes or tighter distributions.

The document covers essential aspects including workflow overviews, best practices for sample handling and reagent use, DNA input recommendations, and guidance on sequencing and data analysis.

Designed for research use, this guide ensures proper and safe application of the Nextera Mate Pair Sample Preparation Kit.

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