Motic PA53 BIO Series: A Practical Guide to Modern Microscopy

Introduction to the Motic PA53 BIO Series

The Motic PA53 BIO Series represents an advanced upright biological microscope, meticulously engineered to meet the demands of modern laboratory environments. It is designed to support a broad spectrum of biological microscopy applications, ranging from clinical diagnostics to advanced academic research.

This series offers precision without compromise, whether for routine observations or capturing high-quality images suitable for publication. The PA53 BIO is tailored for high-end clinical, research, and teaching laboratories that prioritize precision, ergonomics, and modularity.

Key Features and Benefits

• Intelligent Illumination (IL): Automatically remembers light intensity per objective, reducing manual adjustments and enhancing workflow efficiency.
• Kohler Illumination: Features built-in centering tools for uniform contrast and clarity across all magnifications.
• Expandable Fluorescence System: Can be upgraded with fluorescence modules (e.g., FS6) and LUMOS LED light sources for multichannel imaging.
• Research-Grade Optics: Equipped with Motic's Plan UC Achromat or optional Plan Fluor and Plan Apochromatic objectives for superior image sharpness and flatness.
• Digital-Ready Trinocular Design: Facilitates integration with high-resolution cameras and imaging software for digital analysis, documentation, and remote teaching.

Application Areas

• Histology & Cytology: Brightfield imaging with Plan UC Achromats.
• Hematology: 100x oil immersion with a wide field-of-view.
• Pathology / IHC: Plan Fluor objectives for sharp immunostaining.
• FISH / Immunofluorescence: FS6 module with a coded 6-position fluorescence turret.
• Teaching & Documentation: Trinocular head with Moticam camera and software.
• Digital Slide Scanning: Motorized stage and sCMOS camera (PA53 SCAN).

Configurations Overview

• PA53 BIO (Base Model): Ideal for routine brightfield in clinical or teaching labs. Includes Plan UC Achromat objectives (4x-100x), LED illumination, and IL system. Offers upgrade options for phase contrast, darkfield, and polarization.
• PA53 BIO FS6 (Fluorescence Version): Suited for multichannel fluorescence and advanced pathology. Features a 6-position coded fluorescence turret, LUMOS LEDs, and Plan Fluor or Plan APO optics.

Setup & Operation Tips

• Köhler Illumination: Adjust field/aperture diaphragm for crisp contrast.
• Use IL Mode: Set light memory for each objective to streamline imaging.
• Clean Objectives Regularly: Use lens paper and proper immersion oil for 100x objectives.
• Optimize Fluorescence Imaging: Align excitation filters and use anti-fade mounting media.
• Digital Imaging: Utilize Moticam S6 + Motic Images Plus or Image-Pro for capture, measurement, and analysis.

Compatible Add-Ons

• Moticam S6 or S12 Cameras
• C-mount adapters (0.35x to 0.65x)
• Image-Pro Software (for life science analysis)
• Phase Contrast Slider Kit
• FS6 Fluorescence Upgrade Kit

Technical Specifications

The Motic PA53 BIO Series is available in various configurations, including Basic, High-end, PA53 BIO FS6, and PA53 BIO FS6 EDF, each with distinct specifications regarding objectives, stage, illumination, and accessories. Key models include SKU numbers: 1100104600941, 1100104600947, 1100104600934, and 1100104601152.

For detailed specifications on optical systems, observation tubes, eyepieces, objectives, stage, condenser, illumination, and dimensions, please refer to the technical specifications table within the document.

Why Choose Motic's PA53 BIO?

Scientists are switching to the PA53 BIO for its affordable innovation, flexibility, and responsive global support. It addresses common frustrations with overpriced systems and outdated technology, offering cloud integration, smart imaging, and digital collaboration capabilities.