PromethION 2 Solo Quick Start Guide

Pre-installation Requirements

Before using the PromethION 2 Solo, familiarize yourself with the following resources:

• PromethION 2 Solo User Manual
• Technical Specifications
• Safety and Regulatory Information
• IT Requirements

For detailed information and troubleshooting, refer to the user manual.

What's in the Box

The PromethION 2 Solo package includes:

• PromethION 2 Solo device
• 2 x Configuration Test Cells (CTCs)
• USB Type-C cable
• AC power adapter (country-specific)
• Flow cell cover

The device features a top view with LEDs, flow cell positions, a USB Type-C port, and a power switch (On/Off). The rear panel includes input/output ports.

Installation Guidance

Connecting to a Laptop

1. Ensure your computer meets the IT requirements.
2. Power on your laptop.
3. Install the latest MinKNOW software from the Software Downloads page (community.nanoporetech.com/downloads).
4. Launch MinKNOW by clicking the Nanopore wheel icon on the desktop.

Connecting to GridION

1. Verify your GridION serial number is GXB002 or above.
2. Power on your GridION.
3. Update GridION software to the latest version from the Software Downloads page (community.nanoporetech.com/downloads).
4. Launch MinKNOW by clicking the Nanopore wheel icon on the desktop.

Device Setup

Connect the PromethION 2 Solo to your host (GridION or laptop):

1. Connect the AC power adapter to the mains supply and the device's power port.
2. Ensure no Configuration Test Cells (CTCs) are inserted.
3. Connect the USB Type-C cable to the correct ports on your host and the PromethION 2 Solo. Ensure the P2 icon faces upwards on both connectors.
4. Toggle the power switch on the PromethION 2 Solo to the 'On' position.

Perform a Hardware Check

A hardware check is mandatory before your first sequencing run. This requires your CTCs.

Steps:

1. Load both CTCs into the PromethION 2 Solo flow cell positions and close the device lid.
2. Wait for the flow cell positions to initialize with a blue LED. In MinKNOW, flow cell status indicators will change from grey to white. (Note: LEDs pulse white then turn solid blue when CTCs are inserted).
3. In MinKNOW, navigate to 'My device' and select 'Hardware check'.
4. In the Hardware check panel, press 'Select all available positions'. The flow cell status indicators will turn dark blue. (Note: LEDs pulse green during the check).
5. Press 'Start' in the bottom right of the MinKNOW display.
6. Confirm that the flow cell positions show a checkmark (✔️) above the flow cell icon, indicating a successful check.
7. Remove the CTCs from the flow cell positions after the check is complete.

If the hardware check fails, consult the 'Support' section in the Additional Information.

Power Off Procedure

Powering Off the PromethION 2 Solo

1. Remove the PromethION CTCs from the device.
2. Toggle the power switch on the PromethION 2 Solo to the 'Off' position.

Powering Off the Host (Laptop or GridION)

To power off your GridION using MinKNOW, follow the workflow: Navigate to 'Host settings' in the sidebar, then select 'Shutdown' in the main panel or the pop-up box.

You can now close MinKNOW and power off your laptop.

Discover the Nanopore Community and Additional Information

Ensure the success of your sequencing project and stay updated with the latest technology and protocols by visiting the Nanopore Community:

community.nanoporetech.com

Resources Available:

• Training
• Protocols
• Forums
• FAQs (qr.nanoporetech.com/beg1C1)
• Support

Tip: Learn how to analyse your nanopore data at: nanoporetech.com/analyse

Warranty and Ordering:

• Purchase device license and warranty: store.nanoporetech.com/device-warranty.html
• Flow cell warranty: nanoporetech.com/warranty
• Recycle used flow cells: community.nanoporetech.com/support/returns
• Place consumable orders: store.nanoporetech.com

Documentation:

Device documentation is available on the Nanopore Community: community.nanoporetech.com/docs

Customer and Technical Support:

Visit: community.nanoporetech.com/support

Technical Specifications