PromethION 2 Solo Quick Start Guide

Pre-installation Information

Before using the device, familiarize yourself with the following resources:

• PromethION 2 Solo User Manual: community.nanoporetech.com/to/p2solo
• Technical Specifications: community.nanoporetech.com/to/techspec
• Safety and Regulatory Information: community.nanoporetech.com/to/safety
• IT Requirements: community.nanoporetech.com/to/p2solo-it

For detailed information and troubleshooting, refer to the user manual.

Device Overview

The PromethION 2 Solo features the following components:

• Top View: Flow cell cover, Flow cell positions, LEDs.
• Rear Input/Output Panel: USB Type-C port, Power port.
• Included Accessories: 2 x Configuration Test Cells (CTCs), USB Type-C cable, Power adapter.

Installation Guidance

To use the device with a laptop:

1. Ensure your computer meets the IT requirements.
2. Power on your laptop.
3. Install the latest MinKNOW software for MinION Mk1B and PromethION 2 Solo devices from the Software Downloads page (community.nanoporetech.com/downloads). Click the Nanopore wheel icon on the desktop after installation.

To use the device with GridION:

1. Check that the GridION serial number on the label is GXB002 or above.
2. Power on your GridION.
3. Update to the latest GridION software from the Software Downloads page (community.nanoporetech.com/downloads). Click the Nanopore wheel icon on the desktop after updating.

Device Setup

1. Plug the AC power adapter into the mains supply and the other end into the PromethION 2 Solo power port.
2. Ensure no Configuration Test Cells (CTCs) are inserted into the PromethION 2 Solo.
3. Plug the USB Type-C cable into the correct ports on your host laptop or GridION and the other end into the PromethION 2 Solo, with the P2 icon on both USB Type-C connectors facing upwards.
4. Toggle the power switch on the PromethION 2 Solo to the 'On' position.

Perform a Hardware Check

A hardware check is required before performing your first PromethION 2 Solo sequencing run. You will need your CTCs for this procedure.

Hardware Check Overview:

1. Load both CTCs into the PromethION 2 Solo Flow Cell positions and close the device lid.
2. Wait until the flow cell positions initialize with a blue LED. In MinKNOW software, the flow cell status indicators (two boxes) will change from grey to white. (Note: LEDs pulse white then turn solid blue when CTCs are inserted).
3. Press 'Select all available positions'. The flow cell status indicators will change to dark blue. (Note: LEDs pulse green during the hardware check).
4. Press 'Start' in the bottom right of the MinKNOW display.
5. Check for a ✔️ symbol above the flow cell icon in the flow cell positions to indicate a passed hardware check.
6. Remove the CTCs from the flow cell positions after completing the hardware check.

If your hardware check fails, refer to 'Support' in the Additional Information section.

Powering Off the Device

To power off your PromethION 2 Solo:

1. Remove the PromethION CTCs from the device.
2. Toggle the power switch to the 'Off' position.

To power off your laptop or GridION:

To power off your GridION using MinKNOW, follow the workflow in the software (Host settings > Shutdown).

You can then close MinKNOW and power off your laptop.

Nanopore Community

Ensure the success of your nanopore sequencing project and stay up-to-date with the latest technology and protocols. Explore the Nanopore Community for training, protocols, forums, FAQs, and support.

Visit: community.nanoporetech.com

Additional Information

• Warranty: Purchase licenses and warranties at store.nanoporetech.com/device-warranty.html. Flow cell warranty: nanoporetech.com/warranty.
• Recycle Used Flow Cells: Learn how to send your flow cells for recycling at community.nanoporetech.com/support/returns.
• Place Your Next Order: Purchase consumables at the Oxford Nanopore Store: store.nanoporetech.com.
• Documentation: Find device documentation on the Nanopore Community: community.nanoporetech.com/docs.
• Support: For customer and technical support, visit: community.nanoporetech.com/support.

Technical Specifications

Environmental Conditions

• Operating temperature range: +5°C to +40°C.
• Maintain 30 cm clearance to the rear and sides of the device.
• Designed for sequencing in ambient temperatures of +18°C to +25°C.
• Use within 30%-75% relative non-condensing humidity limits.
• Intended for indoor use.
• Can be used up to altitudes of 2,000 m.
• Pollution Degree 2.