Solusi Normalisasi Helix cyto Biosensor Dinamis
Spesifikasi
- Jeneng produk: heliX cyto Normalization Solution (Red Dye)
- Nomer pesenan: NOR-R2
- Kanggo pangukuran scIC ing saluran abang
- Kanggo nggunakake riset mung
- Urip beting winates - priksa tanggal kadaluwarsa ing label
Fitur Utama
- Kanggo normalisasi sinyal fluoresensi ing Spot 1 lan Spot 2 saka chip heliXcyto
- Enables correct real-time referencing of the red fluorescent signals during scIC measurements
- Compatible with all heliXcyto chips
- The Normalization solution (red dye) contains a hydrophilic red dye with a single negative net charge.
Deskripsi Produk
- Nomer pesenan: NOR-R2
Tabel 1. Isi lan Informasi Panyimpenan
| Bahan | Cap | Konsentrasi | Jumlah | Panyimpenan |
| Solusi normalisasi-R2 | abang | 10 µM | 6 x 100 µL | -20°C |
- Kanggo nggunakake riset mung.
- This product has a limited shelf life; please see the expiry date on the label.
- To avoid many freeze-thaw cycles, please aliquot the solution.
Persiapan
- Use this red dye normalization solution for scIC measurements in the red channel (analyte label-dependent).
- Dilute the 10 µM normalization stock solution to a working concentration with running buffer.
- The concentration of the normalization solution should approximately correspond to the fluorophore concentration in the highest analyte concentration to be measured. This can be calculated usingthe following equation:
Concentration of the normalization solution in the desired color :
Konsentrasi pewarna ing larutan analit berlabel
Highest concentration of the analyte that should be measured
Degree of labeling (ratio of dye to analyte)
Solusi sing diencerke bisa disimpen ing 2-8 ° C nganti 7 dina.
Cathetan Aplikasi
In the scIC measurement, the fluorescent signal of the normalization solution should be in a similar range to the highest signal coming from the bound analyte (raw data). The absolute fluorescent signal is dependent on normalization solution concentration and the excitation power applied in the measurement. The excitation power has to be selected based on the following parameters:
- Fluorophore concentration inthe analyte solution
Konsentrasi fluorofor gumantung saka konsentrasi analit sing digunakake ing pangukuran uga tingkat labeling analit. Kanggo DOL dhuwur lan konsentrasi analit sing dhuwur, nyuda daya eksitasi bisa uga dibutuhake. - Sinyal naleni sing dikarepake
Highly expressed targets on a cell can bind more molecules of the labeled analyte. In case of highly overexpressed targets, a strong binding signal can be expected. To avoid the shutter closing, lowering the excitation power might be considered. - Jinis chip
Jinis chip sing beda duwe latar mburi neon sing beda-beda. Sing luwih gedhe traps lan liyane traps ing chip, sing luwih dhuwur sinyal latar mburi. Mulane, chip L5 bisa uga mbutuhake daya eksitasi sing luwih murah tinimbang sing ditrapake ing chip M5.
For a starting point of excitation power and normSolution on concentration to be used in an scIC experiment, please refer to Table 2.
Table 2. Relation of fluorophore concentration, normalization solution concentration, and excitation power suitable for an 
M5 chip
| Pewarna Analit Konk. = analit conc x DOL | Daya eksitasi | Solusi Normalisasi Konsentrasi | Solusi normalisasi pengenceran |
| 25 nM | 0.5 | 25 nM | 1:400 |
| 50 nM | 0.3 | 50 nM | 1:200 |
| 100 nM | 0.2 | 100 nM | 1:100 |
| 300 nM | 0.1 | 300 nM | 1:33 |
| 500 nM | 0.08 | 500 nM | 1:20 |
| 1 µM | 0.05 | 1 µM | 1:10 |
| 2.5 µM | 0.02 | 2.5 µM | 1:4 |
Cathetan: This table is for your guidance. However, the final signal recorded in the heliXcyto depends on many factors. Thus, some optimization will be required for each system.
Kontak
Dynamic Biosensors GmbH
Perchtinger Str. 8/10 81379 Munich, Germany
Bruker Scientific LLC
40 Manning Road, Manning Park, Billerica, MA 01821 USA
- Informasi pesenan
- Dhukungan Teknis
www.dynamic-biosensors.com
Instrumen lan chip direkayasa lan diprodhuksi ing Jerman. ©2025 Dynamic Biosensors GmbH Mung Kanggo Riset. Ora digunakake ing prosedur diagnostik klinis.
Pitakonan
What is the shelf life of the heliX cyto Normalization Solution?
Produk kasebut nduweni umur simpan sing winates. Mangga dipriksa tanggal kadaluwarsa ing label kanggo informasi tartamtu.
How should I store the NOR-R2 v1.0 product?
The product should be stored according to the storage information provided in Table 1 of the user manual.
Can I use this product for clinical purposes?
No, this product is for research use only.
Dokumen / Sumber Daya
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Solusi Normalisasi Helix cyto Biosensor Dinamis [pdf] Manual pangguna helix cyto Solusi Normalisasi, helix cyto, Solusi Normalisasi, Solusi |