Hygiena foodproof® Alicyclobacillus Detection LyoKit

Accurate and Efficient Detection of Alicyclobacillus in Food Products

Introduction to Alicyclobacillus

The genus Alicyclobacillus comprises Gram-positive, acidophilic, and thermophilic, spore-forming bacteria commonly found in soils. These spores can persist in food manufacturing environments, often introduced via contaminated fruit, and survive standard pasteurization processes. While non-pathogenic, these bacteria can impart undesirable disinfectant-like off-flavors, such as guaiacol and halophenols, to final products, particularly affecting fruit juices, concentrates, and preparations, including tomato-based items. Alicyclobacillus acidoterrestris is the most prevalent contaminating species within this genus, though other species have been identified in recent years.

foodproof® Alicyclobacillus Detection LyoKit Overview

The foodproof® Alicyclobacillus Detection LyoKit utilizes real-time PCR technology, a well-established method in the food industry. This kit facilitates the detection and identification of the Alicyclobacillus genus and specifically targets all guaiacol-producing species in a single assay.

Key Features and Benefits

Best Specificity:
- 100% Inclusivity: Tested against 63 Alicyclobacillus strains from 20 species, including all six guaiacol-producing species.
- 100% Exclusivity: Validated against 46 strains, including closely related organisms and microorganisms commonly found in juice and fruit products.
Sensitive: Offers a high-sensitivity procedure, benchmarked against the International Fruit and Vegetable Juice Association (IFU) method no. 12 (BAT medium).
Safe: Minimizes the risk of false-negative results through internal controls and the use of Uracil-N-Glycosylase to prevent carry-over contamination.
Fast: Provides results within a short 4-hour timeframe with a direct sample protocol, requiring minimal hands-on time. Presence/absence confirmation is available after 24 to 48 hours of enrichment, potentially saving up to 5 days compared to IFU Method 12 guidelines.
Easy: A convenient, complete solution that includes DNA extraction and real-time PCR analysis.
Matrices: Tested with a wide range of food matrices, including fruit juice, fruits, concentrates, syrups, tomato paste, and fruit-based spreads.
Identification of Species: Enables the identification of all guaiacol-producing Alicyclobacillus species, including A. acidoterrestris and A. suci, within a single assay. Features an easy and convenient direct sample protocol for juices, concentrates, and other products.

Workflow

The detection process involves three main stages:

Direct Sample or Enrichment: This initial step requires 24 to 72 hours.
DNA Extraction: Approximately 30 minutes are needed for DNA extraction.
Real-Time PCR: The final analysis takes approximately 90 minutes.

[Workflow diagram description: The process is illustrated in three steps. Step 1 shows a sample bag for 'Direct Sample or Enrichment' (24-72 hours). Step 2 shows a test tube for 'DNA Extraction' (approx. 30 min). Step 3 shows a computer screen for 'Real-Time PCR' (approx. 90 min).]

Product Components and Kits

DNA Extraction Kits

foodproof® StarPrep Two Kit
Enables fast and easy extraction of Alicyclobacillus DNA from food and beverage matrices.
Model Number: KIT230177
Tests: 96

Real-Time PCR Kits

foodproof® Alicyclobacillus Detection LyoKit
(Identification of all guaiacol producers)
Model Numbers: KIT230151 (LP), KIT230152 (RP)
Tests: 96

Additional Reagents

Color Compensation Set 5
(Calibration tool for assays on LightCycler 480 System)
Model Number: KIT230011
Reactions: 96

Instrument Compatibility

The kits are compatible with various real-time PCR instruments:

Low Profile (LP) instruments: e.g., LightCycler® 480, LightCycler® 96, AriaMx, Bio-Rad CFX96™, Applied Biosystems™ 7500 Fast, QuantStudio™.
Regular Profile (RP) instruments: e.g., Mx3005P.

Calibration Use: LightCycler 480 System.

Hygiena Information

Hygiena®
Camarillo, CA 93012
USA
Website: www.hygiena.com
Support Email: diagnostics.support@hygiena.com

Trademarks: foodproof®, microproof®, vetproof®, RoboPrep®, and LyoKit® are trademarks of Hygiena Diagnostics GmbH. Hygiena® is a registered trademark of Hygiena. Other brand or product names are trademarks of their respective holders.

Document Version: KD-ALI-01-EN V03/2022

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