Hygiena foodproof® Salmonella plus Cronobacter Detection LyoKit
Product Overview
The foodproof® Salmonella plus Cronobacter Detection LyoKit is a real-time PCR assay designed for the rapid detection of Salmonella and Cronobacter species. These pathogens are recognized as high-risk bacteria in infant formula and medical nutrition products, where consumer resistance is low, necessitating accurate detection results.
This kit utilizes established methods for rapid pathogen detection in the food industry and can simultaneously detect Salmonella and Cronobacter. It is suitable for testing raw materials, infant formula, and environmental samples. The kit also includes an internal positive control for reliable interpretation of results, even in the absence of target analytes.
Key Benefits
- Reduced Time and Cost: Offers a more efficient laboratory workflow.
- Dual Detection: Provides results for both Salmonella and Cronobacter in a single reaction.
- Simplified Workflow: Uses a common enrichment and simplified DNA extraction protocol for ease of use.
- Rapid Results: Delivers definitive positive or negative results in as little as 19 hours, significantly reducing testing time compared to traditional culture methods.
Accuracy and Reliability
- Comprehensive Coverage: Detects 100 strains of Salmonella and 50 strains of Cronobacter with 100% inclusivity.
- High Specificity: Demonstrates zero cross-reactivity with 30 non-target strains.
- Consistent Results: Verified by multiple laboratory studies for consistent reproducibility.
- Lyophilized Technology: Features pre-dispensed, lyophilized PCR reagents to minimize cross-contamination risks associated with liquid handling.
Certifications
The kit holds AFNOR certification (ISO) - QUA 18/12/24 and QUA 18/13/24, valid for infant formula with or without probiotics and related ingredients, and environmental samples (up to 375g test portions).
Certification Details:
- AFNOR Certification: [NF Validation EN ISO 16140]
- Reference Numbers: QUA 18/12-12/24, QUA 18/13-12/24
- Application: Alternative analytical methods for agricultural enterprises.
- More Information: http://nf-validation.afnor.org/en
Product Information
| Product Number | Description | Quantity | Compatible Instruments |
|---|---|---|---|
| KIT230131 | foodproof Salmonella plus Cronobacter Detection LyoKit (LP) | 96 | BAX® System Q7, LightCycler 480 |
Workflow
The detection process involves three main steps:
- Enrichment: 16-24 hours at 37 ± 1 °C.
- DNA Extraction: Time varies depending on the protocol.
- Real-time PCR: Approximately 100 minutes.
DNA Extraction Kits
The foodproof Salmonella and Cronobacter detection kits are compatible with various extraction methods, offering flexibility. Please choose the extraction kit that best suits your laboratory's needs:
| Product Number | Description | Quantity |
|---|---|---|
| KIT2047 | BAX® Lysis Buffer | 96 |
| KIT230187 | foodproof StarPrep® Three Kit (Standard) | 96 |
| KIT230188 | foodproof StarPrep® Three 8-Strip Kit (High Throughput) | 480 |
Related Products
Hygiena also offers a range of other products, including sample collection devices, enrichment media, and other related detection methods, to facilitate the detection of pathogens in environmental samples and finished products.
| Product Number | Description | Quantity |
|---|---|---|
| SS100BPWP | Stick Sponge™ Pre-moistened 10 mL Buffered Peptone Water | 100 |
| SS100NBP | Stick Sponge™ Pre-moistened 10 mL Neutralizing Buffer | 100 |
| MS1-EB | MicroSnap® Enterobacteriaceae EB Enrichment Device | 100 |
| MS2-EB | MicroSnap® Enterobacteriaceae EB Detection Device | 100 |
| MED2011 | Hygiena Buffered Peptone Water (BPW) - Dehydrated Culture Medium | 500 g |
| MED2010 | Hygiena Buffered Peptone Water (BPW) - Dehydrated Culture Medium | 2.5 kg |
| MED2028 | Hygiena Buffered Peptone Water (BPW) - Dehydrated Culture Medium | 80 x 67.5 g packs |
| KIT230043 | foodproof Enterobacteriaceae and Cronobacteriaceae Detection | 96 tests / kit |
| KIT230137 | foodproof Enterobacteriaceae and Salmonella Detection Kit (LP) | 96 tests / kit |
