foodproof® CaMV Detection Kit Ready Reference Guide

Product No. KIT230016

Revision B, September 2025

Manufactured by Hygiena

Introduction

This document provides a ready reference for the foodproof® CaMV Detection Kit, a PCR kit for the qualitative detection of CaMV DNA using real-time PCR instruments. Before starting, it is strongly recommended to read the entire product manual available on the Hygiena website.

PROGRAM SETUP

Program your real-time PCR instrument before setting up the PCR reactions. Select the following channels:

FAM (CaMV)

PCR Program Details:

The PCR program consists of a pre-incubation phase followed by an amplification phase.

Pre-incubation: 1 cycle

Step 1: 37 °C for 4 min
Step 2: 95 °C for 10 min

Amplification: 50 cycles

Step 1: 95 °C for 15* sec
Step 2**: 60 °C for 60 sec

* Use 5 s for LightCycler® 480

** Fluorescence detection

Note: For some real-time PCR instruments, the probe quencher as well as the usage of a passive reference dye has to be specified. This kit contains probes with a non-fluorescent "dark" quencher and no passive reference dye.

DATA INTERPRETATION

Verify results of positive (Control Template) and negative controls (H₂O), before interpreting sample results. Always compare samples to positive and negative controls. Review data from each channel and interpret results as described in the table.

FAM	Result Interpretation
+	Positive for CaMV
-	Negative for CaMV*

* The assay is used in addition to the foodproof® GMO Screening LyoKits and does not contain an internal amplification control. Please check screening results for inhibition control.

PREPARATION OF THE PCR MIX

Take appropriate precautions to prevent contamination, e.g., by using filter tips and wearing gloves. Thaw reagents, mix (do not vortex!), and briefly spin vials before opening.

ADD PCR MIX

Pipet 20 µL of Master Mix into each strip or plate well (for n samples + 2 controls).
ADD SAMPLES AND CONTROLS

Pipet 5 µL of samples, negative control (colorless cap) or Control Template (purple cap) into respective wells.
SEAL

Seal strips/plate accurately.
CENTRIFUGE

Briefly spin strips/plate in a suitable centrifuge.
START REAL-TIME PCR RUN

Cycle samples as described above.

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Introduction

PROGRAM SETUP

PCR Program Details:

DATA INTERPRETATION

PREPARATION OF THE PCR MIX

ADD PCR MIX

ADD SAMPLES AND CONTROLS

SEAL

CENTRIFUGE

START REAL-TIME PCR RUN

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