APERA LogoDO850
Portable Optical Dissolved Oxygen Meter
Instruction ManualAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 21vAPERA INSTRUMENTS (Europe) GmbH

Overview

Thank you for purchasing Apera Instruments DO850 Portable Optical Dissolved Oxygen Meter. The DO850 measures dissolved oxygen in water using luminescence technology through an optical sensor and displays data using advanced intelligent instrument. Compared to electrochemical oxygen analyzers, the DO850 is more accurate and stable, easier to use and cost-effective portable dissolved oxygen meter.
Before you use the instrument, please carefully read the instruction manual to help you properly use and maintain.

1.1 Luminescent optical sensor.

  • Stability and Accuracy: Oxygen is not consumed during measurements. It is not affected by sample flow rate and thus provides a stable measurement.
  • Easy to Use: No electrolytes and membranes are present in the meter; no warm-up; frequent calibration is not necessary.
  • Interference-Free: Sensor cap is coated with a light-shielding layer and minimizes the impact from external light sources. The use of non-chemical sensors helps reduce a
    variety of heavy metal ions interference in the aqueous environment, along with H2S and NH4 and other chemical substances.
  • Long service life. Other than mechanical deterioration (such as scratches to the light shielding layer), the sensor cap has up to 8000 hours of service life.
  • Easy to Calibrate and Maintain. Probe is equipped with calibration/storage sleeve, which makes calibration and maintenance more convenient and reliable.

1.2 Advanced Intelligent Instrument

  • Automatic temperature compensation, automatic pressure compensation and manual salinity compensation.
  • Clear large-size LCD display with white backlight.
  • Reading stable and automatic locking modes.
  • Meets IP57 waterproof rating; in addition, a standard instrument suitcase is provided.

Special Notes

  • Sensor cap surface coating can not withstand high temperatures, so the optical dissolved oxygen electrode can not test the water of more than 50 ℃.
  • Sensor cap must be kept in a humid environment, if the surface coating is dry, treat it by hydration, otherwise the measured value is unstable or the response is slow, as described in section 4.2 (Probe Maintenance)
  • After meter power on, wait about 30 seconds to read value or operatation.

Technical Specifications

Dissolved
Oxygen
Dynamic Range (0-20.00) mg/L (ppm), (0-200.0)%
Resolution 0.01/0.1mg/L (ppm), 0.1/1%
Accuracy ±2% reading or ±2% saturation, whichever is greater
±2% reading or ±0.2 mg/L, whichever is greater
Response Time 30 s (25°C , 90% response)
Calibration Points Saturation Point & Zero Oxygen
Temperature Compensation Automatic, (0 to 50)°C
Pressure Compensation Automatic, (60 to 120) kPa
Salinity Compensation Manual, (0 to 45) ppt
Temperature Range (0 to 50.0) °C
Resolution 0.1 °C
Accuracy ±0.5 °C
Other Batteries AA x 3 (1.5Vx3)
IP Rating IP57
Dimensions and Weight Meter: 88x170x33 mm/313g
With case: 360x270x76 mm/1.3kg
Product Certificate RoHs, CE & IS09001:2015

Instructions

3.1 LCD Screen

APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - LCD Screen

1 Measurement mode icon 7 Auto lock icon
2 Reading/Measured Value 8 Temperature Compensation
ATC — Auto Temperature Compensation
3 Symbol
MTC — Manual Temperature Compensation
4 Units of Measurement
5 Temperature unit 9 Reading Stability Icon
6 Temprature value / Symbol 10 Low Voltage Icon

3.2 Key Operation

APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - LCD Screen 1

Short press: key press time < 2 s; Long press: key press time> 2 s.
Power on: Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 1to turn on. Shutdown: long pressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 1 2 seconds off.
Special notes: After meter power on, wait about 30 seconds to read value or operatation.
Table – 1 Key operation and functions

Key Operation Functions
APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 1 Short Press In the shutdown mode: press the key to boot
In measurement mode: press to turn backlight on or off
Long Press Press and hold for 2 seconds to turn off
APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 2 Short Press In measurement mode: press the key to switch unit: %—>mg/L or %—*ppm
APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 Long Press In the measurement mode: press the key for 2 seconds to enter the calibration mode
Short Press To cancel any operation, press to return to measurement mode
APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 Short Press In the menu mode: press the key to change the serial number or select the parameter
APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4 Short Press In the menu mode: press the key to change the serial number or select the parameter
APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4 Short Press In measurement mode: press to enter menu mode; In calibration mode: press key to calibrate;
In the menu mode: press key to confirm the parameter.

3.3 Batteries
The instrument uses three AA batteries, please use the LR6-type alkaline batteries to ensure battery quality. Battery life> 200 hours (no backlight). When the display showsAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 6 symbol as Figure-3, replace the battery.

APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - LCD Screen 2

3.4 Instrument Socket
The instrument uses an 8-pin socket that is protected by a gray rubber cap seal. When inserting the Probe plug, please insert it after the notch position, and tighten the plug nut. The end face of the socket and the plug connection has the sealing ring, which can effectively maintain the waterproof protection of the socket. Fig.-3
3.5 Reading Stability ModeAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - LCD Screen 3When the measured value is stable, the LCD screen displays the icon as APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 7shown in Figure-4. If there is no icon or APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 7icon flashing, indicating that the measured value is not stable, the measured value should not be read or calibrated.
3.6Auto Lock Mode
In parameter setting P4.2 you can select the auto-lock mode (Off-On),select On to turn on automatic locking. When the reading is stable for more than 10 seconds, the meter automatically locks the measured value and displays the HOLD icon, as shown in Figure 5.
When auto locked, pressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3to unlock.

APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - LCD Screen 4

3.7 Backlighting
The Instruments LCD screen has a white backlight suitable for use in dimly lit environments. Turning on the backlight will consume more power. The instrument is equpped with automatic backlight and manual backlight mode. In automatic backlight mode, when thepower key APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 1 is pressed, backlight will be on for a minute then automatically shuts off; In manual backlight mode, when theAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 1 key is pressed, backlight will be on and will be off APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 1only is pressed again. In the parameter setting P4.3, you can select the auto backlight mode (On-Off), select On to turn on auto backlight, select Off to turn off auto backlight.
3.8 Automatic Power-Off
In the parameter setting P4.4, you can select the auto power off function (On-Off), select On to turn on auto power off, the instrument will shut down automatically after 20 minutes, select Off to disable this function.

Optical Dissolved Oxygen Probe

4.1 Probe Structure
The instruments DO803 optical dissolved oxygen probe has a cable length of 3m and builtin temperature sensor for automatic temperature compensation. The electrode structure is shown in Fig.-6

APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - LCD Screen 5

1. Bottom cover of the calibration sleeve 5. Locking cap
2. Sponge for water storage 6. Optical DO electrode
3. Sensor cap 7. 0 ring
4. Calibration sleeve Immersion line:The tested solution should be above this line

4.2 Probe Maintenance
The sensor cap of the optical DO electrode must be kept in a moist environment. If the surface coating of the sensor cap dries out, the measured value will be unstable or the
response will be slow. The electrode calibration sleeve is used to store the probe.
(A) Short-term storage (less than 30 days): The probe head is kept in the calibration sleeve. Always keep the sponge inside the calibration sleeve wet. Several drops of clean water should be added to a dry sponge (let the sponge be saturated, but not dripping), and tighten the lock cap, so that the sensor cap is kept in the moistsaturated air.
(B) Long-term storage (greater than 30 days): The probe head is kept in the calibration sleeve. Check whether the water storage sponge is moist every 30 days or user can store the electrode in a beaker containing water.
(C) Before the first use, unscrew the calibration sleeve to check if the sponge is wet. If the sponge is dry or if the electrode is exposed to dry air for more than 8 hours, the surface coating of the sensor cap may be completely dry. So the electrode should be immersed in tap water at room temperature 25℃ for 24 hours. If the water temperature is low, soaking time is 48-72 hours.
(D) The sponge can not be allowed to get stained or moldy, otherwise it will consume or produce oxygen. If stained or moldy, please clean immediately.
4.3 Sensor Cap
(A) The sensor cap is an important part of the optical DO probe. The surface coating of the cap cannot be scratched or mechanically worn. Otherwise, the life of the sensor
cap will be reduced or the probe will be damaged. Please pay special attention to it when using the probe.
(B) The surface coating of the sensor cap cannot withstand high temperatures, so the optical DO probe can not be tested in water above 50 ℃.
(C) If the surface of the sensor cap is contaminated, please do not use alcohol and organic solvents to clean, otherwise it may damage the probe. It can be gently wiped with a soft cloth or paper towels. To disinfect the probe, immerse it in 3% hydrogen peroxide for 15 to 30 minutes and then rinse off with water.
(D) The sensor cap has a service life of more than 8000 hours. When the probe is not being used, it does not “bleach” luminescence layer; in addition, the storage time will not reduce the life of the probe, so the actual use time of the sensor cap is far more than a year. The major factor affecting the service life of the sensor cap is the surface coating being damaged under external force. So the key is to protect the sensor cap from external damage.
(E) If the sensor cap is damaged or deteriorated, users need to purchase a new one.
Every new cap has a set of calibration codes which need to be input into the instrument. The specific input method will be described in the instruction manual of sensor cap.
(F) The probe that comes with the instrument can be used directly without the input of the calibration codes. So users should not take off the sensor cap when it is not in use.  Nor should one swap the caps from different instruments. When being installed, the sensor cap must be tightened and the interior can not be contaminated or wet.

Preparation for Calibration

5.1 Dissolved Oxygen Units Selection
Dissolved oxygen units displays in two forms: mg / L and %, and ppm and %. PressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 2  to switch between mg / L → %, or ppm → %. Users can choose mg / L or ppm in parameter setting P3.1, but only a percentage (DO %) is displayed in calibration.
5.2 Resolution Selection
The resolution unit can be selected in parameter setting P3.2: 0.01 or 0.1mg/L (ppm). After setting, the meter will display resolution of 0.1 or 1 in according to %.
5.3 Temperature Unit Selection
The temperature unit can be selected in parameter setting P4.1: ℃ or ℉.
5.4 Air Pressure Compensation
The instrument has automatic air pressure compensation function. The instrument has been performed the air pressure calibration before it leaves the factory. So generally users do not have to calibrate the air pressure. If necessary, calibrate it according to the value of the standard barometer. Refer to parameter setting P3.4 for the procedure of barometric calibration.
5.5 Salinity Compensation
The instrument has manual salinity compensation. It is set in the parameter P3.3 (0 to 45 ppt). To obtain accurate readings in mg/L and ppm, it is necessary to know the salinity of the solution to be tested and input it into the instrument. As the salinity of the solution increases, the level of DO decreases. Generally, salinity of freshwater is 0 to 0.5ppt, salinity of seawater is 35ppt.

Calibration

6.1 Saturated Oxygen Calibration
(A) This procedure requires the use of a calibration sleeve to allow the probe to be calibrated in a humidity-saturated atmosphere
(B) Check that the sponge in the calibration sleeve is wet. Attach the calibration sleeve to the probe. Tighten the locking cap. Be careful not to have water droplets on the head of the cap. Wait for 5 to 10 minutes after turning on the instrument. Saturate the air in the calibration sleeve with water vapor. In addition, wait for the temperature to completely stablize.
(C) Long pressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 the key to enter the calibration mode, and the CAL is flashing in the upper right corner of the LCD. Wait for the stableAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 7 icon to appear and stay, press the key APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4to calibrate, once the instrument displays a stable 100%, it is ready to use.

6.2 Zero -Oxygen Calibration
Zero-Oxygen calibration is only performed when a probe or sensor cap is replaced or the probe has not been used for a long period. It is usually not necessary to conduct zero oxygen calibration. The instrument is factory calibrated for zero oxygen, so it is not necessary to perform a zero-oxygen calibration for the first time. Zero-oxygen calibration should follow these steps:
(A) Preparation of 100ml of oxygen-free water: in the 100ml beaker weigh 2g anhydrous sodium sulfite (Na2SO3) and add 100ml of pure water or tap water to dissolve.
Oxygen-free water is effective within 1 hour.
(B) Put the electrode into the oxygen-free water, wait for 3 to 5 minutes after the instrument is turned on, and wait for the temperature and measured value to completely stabilize. The measured value should be very low, 0.1mg / L or so.
(C) Long press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3to enter the calibration mode. The upper right corner of the LCD will flash CAL . Wait for a stableAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 7 icon. Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4and the zero-oxygen calibration is completed.
6.3 Special Notes for Calibration
(A) Optical dissolved oxygen probes have better stability and smaller calibration drift compared to conventional electrochemical dissolved oxygen electrodes, which means that the instrument can maintain its calibration data for a few months. However, for better accuracy, it is recommended that the oxygen saturation calibration be performed
according to section 6.1 before use every day.
(B) Drying of the surface coating of the sensor cap can adversely affect the stability of the measurement. Please pay special attention to this situation. See Section 4.2 (Probe
Maintenance) for details.
(C) The instrument has factory default setting function, select YES in parameter setting P3.5, the meter will be calibrated to the theory value.

Measurement

7.1 When measuring, place the probe in sample solution, swirl quickly in the solution for a few seconds and rest it to remove bubbles from the measuring surface of the sensor cap. The solution must be above the immersion line of the probe. Note that brief shaking the probe in solution is only to eliminate bubbles. Not like conventional electrochemical electrodes, the measuring via optical dissolved oxygen probes does not require constant stirring of the solution or flowing fluid.
7.2 Users can read the measured values when theAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 7 icon appears and stays. Note that the measurement time is related to temperature. When the solution temperature and the probe temperature is close, the measurement time is about one minute. When the solution temperature and the electrode temperature differ a lot, it takes about 3 minutes to  each a stable reading. This is because the reading of dissolved oxygen is heavily influenced by temperature, and the probe senses temperature much more slowly than dissolved oxygen.

Parameter Settings

8.1 Press theAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4 key in the measurement mode to enter the parameter setting mode P3.0, pressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4 the key to switch the menu P3.0 →P4.0; In P3.0 mode, pressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4 to enter P3.1, pressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 to switch submenu P3.1→P3.5; In P4.0 mode, press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3to enter P4.1, press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 to switch submenu P4.1→P4.4. See Table 2 for details.

Table-2 Parameter Setting List

Menu Submenu Parameter Code Content
P3.0 DO
parameter
P3.1 DO Units Selection / mg/L—ppm
P3.2 Resolution Selection / 0.01/0.1 mg/L(ppm)
P3.3 Salinity Compensation / (0 to 45) ppt
P3.4 Air Pressure Calibration APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 8 (60 to 120) kPa
P3.5 Back to Factory Default APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 9 No—Yes
P4.0 Basic
parameter
P4.1 Temp. Unit Selection / °C °F
P4.2 Auto Lock / Off—On
P4.3 Auto Backlight APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 10 On—Off
P4.4 Auto Power Off APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 11 On—Off

8.2 DO Parameter setting (pressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 orAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4 to switch)

APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 12 P3.1—Dissolved Oxygen Unit (mg/L—ppm)
.In P3.0 mode, press theAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4 key to enter P3.1 mode.
.Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4thekey, mg / L flashes, press theAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3key to select mg/L → ppm, pressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 toAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4confirm. Press to enter P3.2 mode, or press to return to measurement mode.
APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 13 P3.2—Resolution (0.01-0.1mg/L)
1.Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4the key, 0.01 flashes, press the APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3key to select  resolution (0.01- .1mg/L),press to APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4confirm.
2Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3to enter P3.3 mode, or press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3to return to   measurement mode.
APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 14 P3.3—Salinity Compensation (0~45 ppt)
1Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4the key, 0 flashes, Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3or APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3key to adjust salinity value (0 to 45 ppt), Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4Key to confirm.
2Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3to enter P3.4 mode,or press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3to return to  measurement mode.
APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 15 P3.4—Air Pressure Calibration (60 to 120 kPa)
1.Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4thekey, 101.3 flashes, according to standard pressure  value. PressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 orAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 key to adjust, PressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4 key to confirm.
2.Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3to enter P3.5 mode, or pressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 to return to  measurement mode.
APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 15 P3.5—Back to Factory Default (No—Yes)
Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4key, No flashes, PressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3key to select No →Yes,  PressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4key to confirm. Meter returnsAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 to measurement mode. No—cancel restore. Yes—restore

8.3 Basic Parameter setting (press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3orAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 to switch)

APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 18 P4.1— temperature unit (℃—℉)
1. In P4.0 mode, Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4key to enter P4.1 mode.
2.Press  APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4key, °C flashes, PressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 key to select °C→°F,Press key APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4to confirm.
2.Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3key to enter P4.2 mode, or  keyAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 to return to  measurement mode.
APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 18 P4.2—Auto Lock (Off—On)
1Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4key, Off flashes, PressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 key to select Off→On,PressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4 key to confirm. Off—unlock; On—lock (If reading stays stable for more than 10seconds, it auto locks).
2. PressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 key to enter P4.3 mode, APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3or key to return to measurement mode.
APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 20 P4.3—Auto Backlight (On—Off)
1.PressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4 key, On flashes, Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3key to select On→Off, PressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4 key to confirm. On – Auto backlight on, Off – Auto backlight off.
2.Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3key to enter P4.4 mode, or pressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3 key to return to measurement mode.
APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 21 P4.4—Auto Power Off (On—Off)
1. PressAPERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4  key, On flashes Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3key to selectOn→Off,press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4 key to confirm.
On—auto power off on Off—auto power off off
2. Press APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 3key to return to measurement mode

Complete Kit

9.1 What’s in the box

Content Quantity
1. D0850 Portable Optical Dissolved Oxygen Meter 1
2 D0803 Optical Dissolved Oxygen Probe 1
3 Probe Calibration Sleeve 1
4 Small Screwdriver 1
5 Carrying Case 1
6 Sponge for Water Storage (spare) 4
7 Instruction Manual 1

9.2 Accessories for separate purchase

Model Name
D0803 Optical DO probe (3m cable, with sensor cap and calibration sleeve)
D0810 Optical DO probe (10m cable, with sensor cap and calibration sleeve)
D08032 Sensor cap
D08031 Calibration sleeve

Warranty

10.1 We warrant this instrument to be free from defects in material and workmanship and agree to repair or replace free of charge, at option of APERA INSTRUMENTS (Europe) GmbH, any malfunctioned or damaged product attributable to responsibility of APERA INSTRUMENTS (Europe) GmbH for a period of 3 YEARS from the date of purchase. That of DO803 optical DO probe (excluding sensor cap) is 2 years from the date of purchase. That of DO8032 sensor cap is 1 year from the date of purchase.
10.2 Damage and malfunction of the product caused by the following reasons are not covered by the warranty:
(A) Fails to install, operate, or use the product in accordance with the instruction manual, or if the product is damaged by abuse or incorrect use;
(B) The sensor cap is damaged by external force and can not work; or the electrode cable is damaged or twisted due to external force;
(C) Fails to maintain the product in accordance with the requirements of this manual and the industry standard process;
(D) Any unauthorized repairs, and the use of defective or incorrect components to repair the product;
(E) Any modification of the product unauthorized by the Company.
10.3 Product Warranty Period is the free of charge service time for the user who purchase the product, not the service life of the instrument or the probe.

Trouble Shooting

Error Solutions
The instrument does not turn on 1.The battery is not installed correctly. Check the polarity.
2.Battery low voltage, replace the battery.
3.Instrument freezes. Unplug the battery and then install.
The instrument can not calibrate 1.Check calibration procedure: correct atmospheric pressure, salinity input and temperature.
2.The measured value is not stable, prolong the stabilization time, until the demonstration APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 7then presses the APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter - Icon 4key.
3.Check the sensor cap. If it is contaminated, it can be cleaned; if dry, it can be hydrated; if damaged it can be replaced.
DO readings are not accurate 1.Check whether the temperature is stable, the salinity input and barometric pressure are accurate.
2.If the probe calibration is not good, recalibrate.
3.Check the sensor cap. If it is contaminated, it can be cleaned; if dry, it can be hydrated; if damaged it can be replaced.
4.Unscrew the sensor cap, check whether there is moisture inside, if so, wipe off, dry, and tighten it.
The display value stays 200% or 20.0 mg/L. No change 1.Check whether the concentration of the sample is higher than 200% or 20.0 mg / L (ppm).
2.Check if the temperature reading is accurate.
3.If the probe calibration is not good, recalibrate.
4.Check the sensor cap. If it is contaminated, it can be cleaned; if dry, it can be hydrated; if damaged, it can be replaced.

Appendix A: Oxygen Solubility Table (760mm Hg)

Temp °C Chlority: 0
Salinity: 0
5.0 ppt
9.0 ppt
10.0 ppt
18.1 ppt
15.0 ppt
27.1 ppt
20.0 ppt
36.1 ppt

25.0 ppt
45.2 ppt

0.0 14.62 13.73 12.89 12.10 11.36 10.66
1.0 14.22 13.36 12.55 11.78 11.07 10.39
2.0 13.83 13.00 12.22 11.48 10.79 10.14
3.0 13.46 12.66 11.91 11.20 10.53 9.90
4.0 13.11 12.34 11.61 10.92 10.27 9.66
5.0 12.77 12.02 11.32 10.66 10.03 9.44
6.0 12.45 11.73 11.05 10.40 9.80 9.23
7.0 12.14 11.44 10.78 10.16 9.58 9.02
8.0 11.84 11.17 10.53 9.93 9.36 8.83
9.0 11.56 10.91 10.29 9.71 9.16 8.64
10.0 11.29 10.66 10.06 9.49 8.96 8.45
11.0 11.03 10.42 9.84 9.29 8.77 8.28
12.0 10.78 10.18 9.62 9.09 8.59 8.11
13.0 10.54 9.96 9.42 8.90 8.41 7.95
14.0 10.31 9.75 9.22 8.72 8.24 7.79
15.0 10.08 9.54 9.03 8.54 8.08 7.64
16.0 9.87 9.34 8.84 8.37 7.92 7.50
17.0 9.67 9.15 8.67 8.21 7.77 7.36
18.0 9.47 8.97 8.50 8.05 7.62 7.22
19.0 9.28 8.79 8.33 7.90 7.48 7.09
20.0 9.09 8.62 8.17 7.75 7.35 6.96
21.0 8.92 8.46 8.02 7.61 7.21 6.84
22.0 8.74 8.30 7.87 7.47 7.09 6.72
23.0 8.58 8.14 7.73 7.34 6.96 6.61
24.0 8.42 7.99 7.59 7.21 6.84 6.50
25.0 8.26 7.85 7.46 7.08 6.72 6.39
26.0 8.11 7.71 7.33 6.96 6.62 6.28
27.0 7.97 7.58 7.20 6.85 6.51 6.18
28.0 7.83 7.44 7.08 6.73 6.40 6.09
29.0 7.69 7.32 6.93 6.62 6.30 5.99
30.0 7.56 7.19 6.85 6.51 6.20 5.90
31.0 7.43 7.07 6.73 6.41 6.10 5.81
32.0 7.31 6.96 6.62 6.31 6.01 5.72
33.0 7.18 6.84 6.52 6.21 5.91 5.63
34.0 7.07 6.73 6.42 6.11 5.82 5.55
35.0 6.95 6.62 6.31 6.02 5.73 5.46
36.0 6.84 6.52 6.22 5.93 5.65 5.38
37.0 6.73 6.42 6.12 5.84 5.56 5.31
38.0 6.62 6.32 6.03 5.75 5.48 5.23
39.0 6.52 6.22 5.98 5.66 5.40 5.15
40.0 6.41 6.12 5.84 5.58 5.32 5.08
41.0 6.31 6.03 5.75 5.49 5.24 5.01
42.0 6.21 5.93 5.67 5.41 5.17 4.93
43.0 6.12 5.84 5.58 5.33 5.09 4.86
44.0 6.02 5.75 5.50 5.25 5.02 4.79
45.0 5.93 5.67 5.41 5.17 4.94 4.72

Salinity = Dissolved salts in water.
Chlorinity = Measure of chloride content, by mass, of water.
S (‰) = 1.80655 x Chlorinity (‰)
Appendix B: DO % Calibration Values

Cal. value Pressure Cal. value

Pressure

D.O. %

in Hg mmHg kPa mbar D.O. % in Hg mmHg kPa

mbar

101% 30.22 767.6 102.34 1023.38 86% 25.73 653.6 87.14 871.40
100% 29.92 760.0 101.33 1013.25 85% 25.43 646.0 86.13 861.26
99% 29.62 752.4 100.31 1003.12 84% 25.13 638.4 85.11 851.13
98% 29.32 744.8 99.30 992.99 83% 24.83 630.8 84.10 841.00
97% 29.02 737.2 98.29 982.85 82% 24.54 623.2 83.09 830.87
96% 28.72 729.6 97.27 972.72 81% 24.24 615.6 82.07 820.73
95% 28.43 722.0 96.26 962.59 80% 23.94 608.0 81.06 810.60
94% 28.13 714.4 95.25 952.46 79% 23.64 600.4 80.05 800.47
93% 27.83 706.8 94.23 942.32 78% 23.34 592.8 79.03 790.34
92% 27.53 699.2 93.22 932.19 77% 23.04 585.2 78.02 780.20
91% 27.23 691.6 92.21 922.06 76% 22.74 577.6 77.01 770.07
90% 26.93 684.0 91.19 911.93 75% 22.44 570.0 75.99 759.94
89% 26.63 676.4 90.18 901.79 74% 22.14 562.4 74.98 749.81
88% 26.33 668.8 89.17 891.66 73% 21.84 554.8 73.97 739.67
87% 26.03 661.2 88.15 881.53 72% 21.54 547.2 72.95 729.54

APERA INSTRUMENTS (Europe) GmbH
Wilhelm-Muthmann-Straße 18
42329 Wuppertal, Germany
Email: info@aperainst.de
Website: www.aperainst.de
Tel.: +49 202 51988998

Documents / Resources

APERA INSTRUMENTS DO850 Portable Optical Dissolved Oxygen Meter [pdf] Instruction Manual
DO850 Portable Optical Dissolved Oxygen Meter, DO850, Portable Optical Dissolved Oxygen Meter, Optical Dissolved Oxygen Meter, Dissolved Oxygen Meter, Oxygen Meter, Meter

References

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