Fumana mokhoa oa ho hloekisa le ho boloka heliXcyto ea hau ka katleho le li-chips ka CS-100-1 HeliXcyto Fumana Tharollo ea Molecule Cell Cleaning 1. Tharollo ena e entsoeng ka sesepa e etselitsoe ka ho khetheha bakeng sa mekhoa ea ho hloekisa letsatsi le leng le le leng, ho netefatsa ts'ebetso e nepahetseng. Latela litaelo tsa tšebeliso tse fanoeng bukeng ea mosebelisi bakeng sa liphetho tse ntle.
Ithute ho itokisetsa hantle le ho sebelisa heliXcyto RT-IC Running Buffer 1 (RB 1) e nang le mahloriso a 10x stock le 50 mL palo. Netefatsa ts'ebetso e nepahetseng bakeng sa tlhokomelo le litekanyo tsa RT-IC sesebelisoa sa heliXcyto.
Ithute ka PF-BU-B-5 Running Buffer bakeng sa sistimi ea proFIRE bukeng ena ea mosebelisi. Fumana lintlha tsa sehlahisoa, litataiso tsa tšebeliso, litaelo tsa polokelo, le tse ling. Etsa bonnete ba ho ts'oaroa hantle le ho lahla buffer bakeng sa ts'ebetso e nepahetseng.
Ntlafatsa ts'ebetso ea hau ea ho kopanya protheine-DNA ka PF-BU-C-1 Conjugation Buffer ka Dynamic Biosensors. Fumana ka botlalo litaelo tsa tšebeliso le tlhaiso-leseling ea polokelo bukeng ea mosebelisi. Ntlafatsa katleho ea litlhoko tsa hau tsa lipatlisiso ka proFIRE 1X Conjugation Buffer.
Boloka sesebelisoa sa hau sa heliXcyto se le boemong bo botle ka heliXcyto Cleaning Solution 3. Latela litaelo tse nepahetseng bakeng sa tlhokomelo e sebetsang, ho netefatsa nako e telele le ts'ebetso. Nomoro ea Odara: CS-50-3.
Ithute ho sebelisa DNA hantle ka liprotheine tse kholo ho feta 5 KDA u sebelisa PF-SH-1 Thiol Coupling Kit 1 bakeng sa Liprotheine. Bukana ena ea mosebedisi e fana ka ts'ebetso e qaqileng ea mehato ea 3 hammoho le likarolo tsa bohlokoa tse kang lihlopha tsa thiol tse sebetsang hantle le ho hloekisa mela ea spin. Fumana ka lisebelisoa tse khothaletsoang le mehato ea liphetho tse nepahetseng tsa motsoako oa protheine-DNA.
Fumana lintlha tse tebileng ka heliX Plus Adapter Strand 1 ntle le Fluorophore bukeng ena ea mosebelisi. Ithute ka BIOSENSORS e matla le theknoloji e ncha e ka morao ho Strand 1 no Fluorophore. Lekola bokhoni le likarolo tsa sehlahisoa sena se tsoetseng pele bakeng sa litlhoko tsa hau tsa lipatlisiso.
Fumana mokhoa oa ho ntlafatsa Litekanyo tsa RT-IC ho Channel e Tala ka buka ea mosebelisi ea NOR-Ga heliXcyto Normalization Solution (Green Dye). Ithute ka likarolo tsa bohlokoa, boitokisetso, lintlha tsa kopo, le tlhaiso-leseling ea mabitso bakeng sa Dynamic Biosensors GmbH le Inc.
Fumana hore na heliXcyto, tharollo e tloaelehileng (dae e khubelu) ke Dynamic Biosensors GmbH & Inc. (Nomoro ea Taelo: NOR-Ra), e ntlafatsa litekanyo tsa RT-IC mocheng o mofubelu bakeng sa merero ea lipatlisiso. Ithute ka litlhaloso tsa eona, litokisetso, lintlha tsa ts'ebeliso, le melaoana ea polokelo.
Fumana mokhoa oa ho sebelisa DNA ka nepo u sebelisa PF-AB-1 Antibody Oligo Conjugation Kit. Bukana ena ea mosebedisi e fana ka lintlha tse qaqileng tsa mehato e 3 bakeng sa phetoho ea DNA, ho kopanya liprotheine, le ho hloekisa, ho fella ka li-conjugate tsa boleng bo phahameng ba antibody-DNA. Ithute ka lisebelisoa tse hlokahalang, kemiso ea nako, le lintlha tsa bohlokoa bakeng sa kopano e atlehileng.
