Fumana buka ea mosebelisi ea HeliX+ Regeneration Solution bakeng sa nchafatso ea sefahleho sa chip ke Dynamic Biosensors. Ithute ka litlhaloso tsa sehlahisoa, litaelo tsa tšebeliso, le Lipotso-potso bakeng sa SOL-REG-1-5. Boloka sebakeng se pholileng, se omileng hole le khanya ea letsatsi bakeng sa liphetho tse nepahetseng.
Tseba buka ea mosebelisi ea HK-NTA-1 v4.1 HIS Capture Kit ho tsoa ho Dynamic Biosensors. Lekola lintlha tsa sehlahisoa, likarolo tsa bohlokoa, litaelo tsa boitokiso, le lintlha tsa ho ikopanya le tšehetso ea tekheniki le litaelo.
Tseba buka ea mosebelisi ea HK-SA-1 v6.1 Biotin Capture Kit e nang le lintlha le likarolo tsa bohlokoa bakeng sa ho etsa liteko tse sebetsang hantle. Ithute ka bophelo ba shelofo ea sehlahisoa, mehato ea ho lokisa, le mokhoa oa ho nchafatsa chip. Lekola litataiso tse fanoeng ke Dynamic Biosensors bakeng sa tšebeliso e nepahetseng.
Tseba Bukana ea Mosebelisi bakeng sa heliX+ 1X BUFFER C PH 8.0 Coupling Buffer (Nomoro ea Odara: BU-C-150-1 v2.1) ka Dynamic Biosensors GmbH & Inc. Ithute ka sebopeho sa eona, litataiso tsa polokelo, le litaelo tsa tšebeliso ea sehlahisoa bakeng sa nanolever e sebetsang hantle. conjugation.
Fumana Tharollo ea 10X Passivation ka Dynamic Biosensors, ea mohlala SOL-PAS-1-5 v2.1, e etselitsoeng chip surface passivation ka motsoako oa thiol-reactive. Ithute mokhoa oa ho hlapolla hantle le ho nchafatsa tharollo bakeng sa liphetho tse nepahetseng.
Fumana lintlha tse qaqileng le litlhaloso bakeng sa AS-1 Adapter Strand 1 (AS-1-Rc) ka Dynamic Biosensors GmbH & Inc. Ithute ka mahloriso a eona, likarolo tsa bohlokoa, tataiso ea polokelo, le tšebeliso ea ts'ebetso ea biochip. Lekola litekanyo tsa ho kopanya, mehato ea ho incubation, le lisebelisoa tse ling tse hlokahalang bakeng sa ts'ebetso e nepahetseng. Fumana mokhoa oa ho lokisa le ho boloka sehlahisoa sena ka nepo bakeng sa ts'ebeliso ea in-solution hybridization.
Ntlafatsa boima ba ligand ka DC-0 v5.1 Density Control Kit ho tsoa ho Dynamic Biosensors. Fumana liphapang tse nepahetseng tsa ligand holim'a libaka tsa sensor u sebelisa likhoele tsa cAnchor. E nepahetseng bakeng sa heliX+ Adapter Chip Spot 1 le Spot 2. Litaelo tsa polokelo le litaelo tsa tšebeliso li kenyelelitsoe.
The heliX+ AMINE COUPLING KIT 3 by Dynamic Biosensors GmbH e nolofalletsa ho kopana ha limolek'hule tse tlaase tsa pI le li-amine tsa mantlha bakeng sa tlhoekiso ea proFIRE. Bukana ena ea mosebedisi e fana ka litaelo tse qaqileng bakeng sa phetoho ea DNA, ligand conjugation, le mekhoa ea ho hloekisa. E lumellana le mohlala oa HK-NHS-3 v6.1, setlolo sena se fana ka ts'ebetso ea 3-step conjugation workflow le lihlahisoa tse phahameng tsa bohloeki.
Ntlafatsa lipatlisiso tsa liprotheine le li-antibody ka HeliX Plus Reducing Agent Kit 1 (RK-PA-1) ka Dynamic Biosensors. Setsi sena se thusa phokotso ea bond ea disulfide bakeng sa limolek'hule tse fetang 20 kDa, e nolofalletsang phokotso e ikhethileng ea sebaka le ts'ebetso ea ligand ka nako e le 'ngoe. E loketse ho kopanya hanghang mme e kenyelletsa li-reagents bakeng sa maikutlo a mabeli a phokotso.
Ithute ho sebelisa HeliXcyto Normalization Solution ka nepo bakeng sa litekanyo tse nepahetseng tsa fluorescence. Fumana litlhaloso, mehato ea boitokiso, lintlha tsa ts'ebeliso, le Lipotso-potso bukeng ena e felletseng ea mosebelisi. E loketse bafuputsi ba batlang ho netefatsa tlhahlobo e nepahetseng ea data ka heliXcyto NOR-0.
