ALPCO Multi Array Xplorer 45k MAX 45k e Ikemetseng ka Botlalo Litaelo tsa Multiplex Batch Analyzer
- MAX 45k e nolofalletsa samptlhahlobo ea ho araba bakeng sa li-antibodies tse 295 tsa allergen-specific IgE (ALEX2) kapa 286 ea li-antibodies tsa IgG (FOX). Teko ea liphanele ka bobeli e ka phethoa ka 500 µl feela ea mali ho tsoa monoaneng kapa ho huloa mali bakeng sa setsebi se felletseng.file.
- ALEX2 phanele ea allergy e kenyelletsa lintho tse 183 tsa limolek'hule - boholo ba indasteri. Likaroloana tsa limolek'hule tse ikhethang li fana ka tlhahisoleseding e tobileng haholoanyane mabapi le karabelo e ka 'nang ea e-ba teng ea ho kula (lelapa le itseng la allergen) le ho ntlafatsa ho lumellana ha batch-to-batch.
Litšobotsi tsa Seletsa
- Cartridge rotor ❶ e ts'oara likhatriji tse fihlang ho 50 bakeng sa tlhahlobo ea multiplex ea ho fihla ho 50 s.ampka nako e le 'ngoe ea lihora tse 4.
- Nale ea ho hlapa ❷ e eketsa buffer ho li-cartridges tsa motho ka mong ha rotor e phethoha.
- The Imaging Module ❸ e hlahloba khatriji ka 'ngoe ho netefatsa mofuta oa khatriji, nomoro ea lotho, letsatsi la ho felloa ke nako le boemo 'me e nka setšoantšo sa khatriji ka 'ngoe qetellong ea tlhahlobo.
- Letsoho la tšepe la nale ❹ le ea holimo le tlase le li-pivots ho tloha ho sample le reagent rotor ho rotor ea cartridge.
- Setsi sa ho hlatsoetsa se sebetsang ❺ se hlatsoa hantle ntlha ea lipeipi lipakeng tsa setsample phetiso ea reagent ho thibela tšilafalo le ho felisa tlhoko ea malebela a ka lahloang.
- SampLe Rotor e ka amohela ho fihlela ho 50 samp❻ le li-reagents bakeng sa 50sample ALEX2 kapa FOX matha. ❼ Lisebelisoa li kentsoe ka libarcode tse shebileng kantle 'me li baloa ke MAX 45k. ❽
Mokhoa o Tloaelehileng oa Ts'ebetso
Mohato oa 1: Lokisa li-reagents
Laola lisebelisuoa tse ikhethileng tse nang le li-barcode tse shebileng kantle. Netefatsa hore letamo la ho hlatsuoa le tlatsitsoe 'me letamo la litšila ha le na letho.
Mohato oa 2: Laela Samples
Mojaro samptse nang le barcode tse shebileng kantle. 400 µL ea hlokahala bakeng sa sample li-tubes tsa ALEX2. 200 µL e lekane bakeng sa li-tubes tse tlase, tse tlase. Sampli-les li hlapolotsoe pele ho sebelisoa 12 µL ea sample bakeng sa tlhahlobo ea FOX. Boholo ba vial bo ka kopanngoa.
Mohato oa 3: Kenya li-cartridges
Li-cartridges li laeloa ho tloha bohareng ho sutumelletsa ka ntle ho fihlela molumo oa ho penya o utloahala ho qala sebakeng sa 1.
Mohato oa 4: Koala Li-Lids le ho Laola Inventory
Nakong ea tsamaiso ea thepa, MAX 45k e tla rekota reagent, sample, le tlhaiso-leseling ea cartridge ho tsoa ho li-barcode le maemo a log.
Mohato oa 5: Etsa Likhetho tsa Priming le Predilution 'me u netefatse Meqolo ea Reagent
Kgetha priming e tlwaelehileng kapa e atolositsweng mme o bontshe haeba tsohle sampli se li hlophisitsoe esale pele. Nakong ea netefatso, MAX 45k e tla netefatsa palo e lekaneng bakeng sa lisebelisoa tsohle tsa tlhahlobo.
Mohato oa 6: Qala ho Run
MAX 45k e tla iketsetsa mehato eohle ea dilution, pipetting, incubation, hlatsoa le ho nka khatriji. Ha tlhahlobo e felile, sebaka sa boemo se tla bontša "Test Run Finished". Ka ho tobetsa "View Litekanyo”, leqephe la sephetho lea buloa.
Liphanele tsa Array
ALEX2 Allergy Xplorer:
~300 allergens, ho kenyeletsoa 183 ea limolek'hule allergens
- ALEX2 phanele e kenyelletsa 144 recombinant molecular allergens le ntho e thibelang e matla ho thibela litlamo tse sa totobalang le tse ka bang teng ka lebaka la tsoekere e sebetsang (CCDs kapa li-cross-reactive carbohydrate determinants) ho lintho tse ling tse amanang le liphetho tse nepahetseng.
FOX Food Xplorer:
Lijo tse 283 tse akaretsang lihlopha tse 13 tsa lijo
- Phanele ea FOX e lekanya li-antibodies tsohle tse ikhethileng tsa IgG, ho kenyeletsoa li-immune-activating IgG1 le IgG3 subclass, li-immune-modulating IgG2 antibodies, le li-antibodies tsa IgG4 tse thibelang 'mele bakeng sa ho lemoha ho se mamelle lijo kapa mamello ea lijo.
Re leboha ho ba teng pontshong ya ALPCO ya Max 45k ho AACC. Ka kopo ikopanye sales@alpco.com ho hlophisa nehelano ea lijo tsa mots'eare le ho ithuta ho buisana ho ea pele hore na Max 45k e kena lab ea hau joang.
sales@alpco.com | 800-592-5726 | www.alpco.com
13JUL22
Litokomane / Lisebelisoa
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