Hep-G2/2.2.15 Human Hepatoblastoma Cell Line - Safety Data Sheet

Section 1: Identification

1.1 Product Identification

Product Name: Hep-G2/2.2.15 Human Hepatoblastoma Cell Line

Product Code: SCC249

Product Number: 9Q073Q

Brand: Millipore

1.2 Supplier Details

Manufacturer/Supplier: Sigma-Aldrich (Shanghai) Trading Co.Ltd.

Address: 509 Renqing Road, Zhangjiang High Tech East Park, Pudong, SHANGHAI 201201 SHANGHAI, CHINA

Also listed: Sigma-Aldrich (Shanghai) Trading Co. Ltd., 509 Renqing Road, Shanghai, Postal Code: 201201, CHINA

Parent Company: Merck KGaA, Darmstadt

Note: The life science business of Merck operates as MilliporeSigma in the US and Canada.

Section 1.3 Emergency Contact

Phone: +49(0)6151 72-2440 (Germany)

Contact Phone: +86 21 6141-5566

Fax: +86 21 6141-5567

Emergency Consultation Number: +86 532 83889090 (NRCC) / 400 120 4937 (CHEMTREC)

Section 1.4 Recommended Use and Restrictions

Recommended Use: Biochemical research/analysis

Section 2: Hazard(s) Overview

Emergency Overview: Liquid. Color: Light pink to red. Not a hazardous substance or mixture.

GHS Hazard Classes: Not a hazardous substance or mixture.

GHS Label Elements: No pictograms, signal words, hazard statements, or precautionary statements are required.

Physical and Chemical Hazards: Not classified based on available information.

Health Hazards: Not classified based on available information.

Environmental Hazards: Not classified based on available information.

Other Hazards Not Covered by GHS: Although it is unknown whether the cell line contains pathogens that can harm humans or contaminate the environment, the possibility of adventitious viruses is rarely excluded. Therefore, it is recommended that all animal/human cell lines be handled under Biosafety Level 2 (BSL-2) containment [US Government publication "Biosafety in Microbiological and Biomedical Laboratories (BMBL) 5th Edition" (CDC, 2009)].

Section 3: Composition/Information on Ingredients

Substance/Mixture: Mixture

Components

Section 4: First-Aid Measures

Inhalation: Move to fresh air. If feeling unwell, consult a doctor.

Skin Contact: In case of skin contact, immediately remove/take off all contaminated clothing. Wash skin/shower with water. Wash with a suitable disinfectant.

Eye Contact: After eye contact, rinse thoroughly with plenty of water. Remove contact lenses.

Ingestion: After ingestion, immediately let the injured person drink water (up to 2 cups). If necessary, consult a doctor.

Most important symptoms and effects: The most important known symptoms and effects are described in the label (see Section 2.2) and/or Section 11.

Advice to rescuers: See Section 8 for personal protection.

Special advice to doctors: No data available.

Section 5: Fire-Fighting Measures

Extinguishing Media and Fire Extinguishing Agents: Use extinguishing measures appropriate to the surrounding environment and situation.

Unsuitable Extinguishing Media: No restrictions on extinguishing agents are specified for this substance/mixture.

Specific Hazards: Non-flammable. May produce hazardous gases or vapors when ignited.

Hazardous Combustion Products: Carbon oxides, Nitrogen oxides, Sulfur oxides, Hydrogen chloride gas, Calcium oxide.

Special Fire-Fighting Procedures: Prevent fire-fighting water from polluting surface and groundwater.

Special Protective Equipment for Firefighters: In case of fire, wear self-contained breathing apparatus.

Section 6: Accidental Release Measures

Personal Precautions, Protective Equipment and Emergency Procedures:

• Advice for non-emergency personnel: Avoid direct contact with biological material. Do not inhale vapor/aerosol. Evacuate hazardous areas, follow emergency procedures, seek expert advice.
• Advice for emergency responders: See Section 8 for personal protection.

Environmental Precautions: Do not let product enter drains.

Methods for Containment and Cleaning Up: Cover drains. Collect, dike, pump out spilled material. Use absorbent material (e.g., Merck Chemizorb®) to absorb liquid. Dispose of as chemical waste. Clean affected areas. Avoid inhaling vapor. Use effective purification methods such as autoclaving, incineration, or chemical disinfection to clean and purify all materials to be treated, as well as contaminated surfaces.

Follow applicable material restrictions (see Sections 7 and 10).

Section 7: Handling and Storage

Handling Precautions: For precautions, see Section 2.2.

Advice on Fire Prevention and Explosion Prevention: Handle materials in a Biosafety Level 2 (BSL-2) containment facility.

Safety Disposal Precautions: See Section 8 for personal protection.

Storage

Storage Precautions: Keep tightly closed.

VCI Storage Class: 12, Non-flammable liquid.

Recommended Storage Temperature: Refer to product label.

Section 8: Exposure Controls/Personal Protection

Exposure Limits and Occupational Exposure Limits for Components

Other Information: Skin

Engineering Controls

No data available.

Personal Protective Equipment

Respiratory Protection: Required when vapor/mist is generated.

We recommend respiratory protection filters based on standards: DIN EN 143, DIN 14387, and other relevant standards for respiratory protective devices.

Recommended Filter Type: Protective equipment with Type A organic vapor filter (according to DIN 3181 definition).

Employer must maintain, clean, and test respiratory protective equipment according to the manufacturer's instructions. These measures must be properly documented.

Eye/Face Protection: Use eye protection equipment tested and approved according to official standards such as NIOSH (USA) or EN 166 (EU). Safety glasses.

Skin and Body Protection: The type of protective equipment must be selected based on the concentration and quantity of hazardous substances in the specific workplace.

Hand Protection

Chloroprene (Neoprene) Gloves

• Material: Chloroprene
• Solvent Permeation Time: 480 minutes
• Glove Thickness: 0.65 mm
• Protection Index: Full contact
• Manufacturer: KCL 720 Camapren®

Latex Gloves

• Material: Latex
• Solvent Permeation Time: 240 minutes
• Glove Thickness: 0.6 mm
• Protection Index: Splash protection
• Manufacturer: Lapren® (KCL 706 / Aldrich Z677558, Size M)

Note: This recommendation is only applicable to products supplied by us and listed on the Safety Data Sheet for their intended use. If dissolved or mixed with other substances, or if deviations from EN 16523-1 occur, contact a CE-approved glove supplier (e.g., German glove supplier KCL Company, website: www.kcl.de).

Hygiene Measures: Standard industrial hygiene practices. Change contaminated clothing.

Section 9: Physical and Chemical Properties

Appearance and State: Liquid

Color: Light pink to red

Odor: No data available

Odor Threshold: No data available

pH Value: No data available

Melting Point: No data available

Boiling Point/Boiling Range: No data available

Flash Point: No data available

Evaporation Rate: No data available

Flammability (Solid, Gas): No data available

Flammability (Liquid): This product is not flammable.

Burning Rate: No data available

Explosion Limit/Flammability Limit: No data available

Explosion Lower Limit/Flammability Lower Limit: No data available

Vapor Pressure: No data available

Vapor Density: No data available

Relative Density: No data available

Density: No data available

Solubility:

• Water Solubility: Soluble (20 °C)

Octanol/Water Partition Coefficient: No data available

Auto-ignition Temperature: No data available

Decomposition Temperature: No data available

Dynamic Viscosity: No data available

Kinematic Viscosity: No data available

Flow Time: No data available

Explosive Properties: Not explosive.

Oxidizing Properties: No

Particle Characteristics:

• Particle Size: No data available

Section 10: Stability and Reactivity

Reactivity: No data available

Chemical Stability: No data available

Possibility of Hazardous Reactions: May react violently. Generally considered to react with water.

Conditions to Avoid: No data available

Incompatible Materials: No data available

Hazardous Decomposition Products: In case of fire: see Section 5 Fire-fighting measures.

Section 11: Toxicological Information

11.1 Toxicological Effects of the Mixture

Acute Toxicity

• Oral: No data available
• Inhalation: No data available
• Dermal: No data available

Skin Corrosion/Irritation: No data available

Serious Eye Damage/Eye Irritation: No data available

Respiratory or Skin Sensitization: No data available

Germ Cell Mutagenicity: No data available

Carcinogenicity: No data available

Reproductive Toxicity: No data available

Specific Target Organ Toxicity (Single Exposure): No data available

Specific Target Organ Toxicity (Repeated Exposure): No data available

Aspiration Hazard: No data available

11.2 Additional Information

No data available.

Component: Dimethyl sulfoxide

Acute Toxicity

• LD50 Oral - Rat - Male and Female: 28,300 mg/kg (OECD Test Guideline 401)
• LC50 Inhalation - Rat - Male and Female: 4 h - > 5.33 mg/l - Dust/Mist (OECD Test Guideline 403)
• LD50 Dermal - Rat - Male and Female: 40,000 mg/kg
• Note: (ECHA)

Skin Corrosion/Irritation

• Skin - Rabbit: Result: Mild irritant - 4 h (OECD Test Guideline 404)

Serious Eye Damage/Eye Irritation

• Eyes - Rabbit: Result: Mild irritant - 24 h (OECD Test Guideline 405)

Respiratory or Skin Sensitization

• Maximization Test - Guinea Pig: Result: Negative (OECD Test Guideline 406)
• Local Lymph Node Assay (LLNA) - Mouse: Result: Negative (OECD Test Guideline 429)

Germ Cell Mutagenicity

• Test Type: Ames Test
• Test System: Salmonella typhimurium
• Result: Negative
• Test Type: Sister Chromatid Exchange Test
• Test System: Chinese Hamster Ovary Cells
• Result: Negative
• Test Type: Mutagenicity (Mammalian Cytogenetic Test): Chromosomal Aberration Negative.
• Test System: Chinese Hamster Ovary Cells
• Result: Negative
• Method: OECD Test Guideline 474
• Species: Rat - Male and Female
• Result: Negative

Carcinogenicity: No data available

Reproductive Toxicity: No data available

Specific Target Organ Toxicity (Single Exposure): No data available

Specific Target Organ Toxicity (Repeated Exposure): No data available

Aspiration Hazard: No data available

Section 12: Ecological Information

Ecotoxicity

Component: Dimethyl sulfoxide

• Toxicity to Fish: LC50 (Danio rerio (Zebrafish)): > 25,000 mg/l
• Endpoint: Mortality
• Exposure Time: 96 h
• Test Type: Static Test
• Analytical Monitoring: Yes
• Method: OECD Test Guideline 203
• GLP: Yes
• Toxicity to Aquatic Invertebrates and Other Aquatic Organisms: EC50 (Daphnia magna): 24,600 mg/l
• Exposure Time: 48 h
• Test Type: Static Test
• Analytical Monitoring: Yes
• Method: OECD Test Guideline 202
• Toxicity to Algae/Aquatic Plants: ErC50 (Pseudokirchneriella subcapitata): 17,000 mg/l
• Exposure Time: 72 h
• Test Type: Static Test
• Analytical Monitoring: Yes
• Method: OECD Test Guideline 201
• GLP: Yes
• Toxicity to Microorganisms: EC50 (Activated Sludge): 10 - 100 mg/l
• Exposure Time: 30 minutes
• Method: ISO 8192

Persistence and Degradability

Component: Dimethyl sulfoxide

• Biodegradability: Aerobic
• Concentration or Concentration Range: 2 mg/l
• Result: Not readily biodegradable.
• Biodegradation Rate: 31%
• Exposure Time: 28 d
• Method: OECD Test Guideline 301D
• GLP: Yes

Abiotic Degradation

• Hydrolysis Half-life: 0.12 - 1.2 h (30 °C) pH: 7
• Note: Readily hydrolyzable.

Bioaccumulative Potential

Component: Dimethyl sulfoxide

• Octanol/Water Partition Coefficient: log Pow: -1.35 (20 °C)
• Note: Predicted to have low bioaccumulation potential.

Mobility in Soil: No data available

Other Adverse Environmental Effects: No data available

Section 13: Disposal Considerations

Disposal Methods

Waste Chemicals: Used materials and any products containing infectious materials must be autoclaved and disposed of according to all local and national regulations.

Dispose of remaining and non-recyclable solutions through a licensed company.

Contaminated Packaging: Dispose of as unused product.

Section 14: Transport Information

International Regulations

Air Transport (IATA-DGR)

• UN/ID Number: UN 3373
• Proper Shipping Name: Biological substance, Category B
• Class: 6.2
• Packing Group: Not regulated
• Label:
• Packing Instructions (Cargo Aircraft): 650
• Packing Instructions (Passenger Aircraft): 650

Sea Transport (IMDG-Code)

• UN Number: UN 3373
• Proper Shipping Name: BIOLOGICAL SUBSTANCE, CATEGORY B
• Class: 6.2
• Packing Group:
• Label: 3373
• EmS Number: F-A, S-T
• Marine Pollutant (Yes/No): No
• According to MARPOL73/78 Annex II and IBC Code: Not applicable to the product supplied.

Domestic Regulations

JT/T 617

• UN Number: UN 3373
• Proper Shipping Name: Biological substance, Category B
• Class: 6.2
• Packing Group: Not regulated
• Label: 6.2
• Environmentally Hazardous: No

Special Precautions: The transport classification provided herein is for reference only and is based solely on the nature of the unpacked material described in this Safety Data Sheet. Transport classification may vary depending on the mode of transport, package size, and regional or national regulations.

Section 15: Regulatory Information

Applicable Regulations

Occupational Disease Prevention Law

Regulations on the Safety Management of Hazardous Chemicals

• Hazardous Chemicals Catalogue: Not applicable
• List of Key Regulated Hazardous Chemicals: Not applicable

Regulations on the Management of Environmental Protection for Import and Export of Toxic Chemicals and Hazardous Chemicals

• Catalogue of Toxic Chemicals Strictly Restricted for Import and Export in China: Not applicable

Measures for the Environmental Management Registration of New Chemical Substances

• Registration Certificate/Filing Receipt Number: B1A222214443, B1A222225559, B1A222221992, B1A122210525, B1A222221985, B1A222250127

Downstream users should comply with the intended uses, environmental and health hazard characteristics, and environmental risk control measures listed in the Safety Data Sheet, as well as local/national regulations for relevant chemicals.

Regulations on the Management of Precursor Chemicals: Not listed.

Section 16: Other Information

Revision Date: 2025/08/12

Other Information:

• Copyright: 2025 Sigma-Aldrich Co. LLC. All rights reserved. Paper copies may be made for internal use only.
• The information provided is believed to be correct but does not purport to be all-inclusive and shall be used only as a guide. The information in this document is based on our current knowledge and is applicable to the product as described. It does not represent a guarantee of the product's properties. Sigma-Aldrich and its affiliates shall not be liable for any damage resulting from handling or contact with the above product. For further terms of use, see the reverse side of the invoice or packaging slip. For further sales terms and conditions, see www.sigma-aldrich.com and/or the reverse side of the invoice or packing slip.
• Date Format: Year/Month/Day
• Abbreviations and Acronyms:
• GBZ 2.1-2007: Occupational exposure limits for hazardous factors in the workplace - Chemical hazardous factors
• GBZ 2.1-2007 / PC-TWA: Time-weighted average permissible concentration
• AIIC - Australian Inventory of Industrial Chemicals; ANTT - Brazilian National Land Transport Agency; ASTM - American Society for Testing and Materials; bw - body weight; CMR - Carcinogenic, Mutagenic or Reprotoxic substance; DIN - German Institute for Standardization; DSL - Domestic Substances List (Canada); ECx - Concentration of effect leading to x% response; ELx - Effect level leading to x% response; EmS - Emergency Schedule; ENCS - Existing and New Chemical Substances (Japan); ErCx - Concentration of growth rate leading to x% effect; ERG - Emergency Response Guidebook; GHS - Globally Harmonized System of Classification and Labelling of Chemicals; GLP - Good Laboratory Practice; IARC - International Agency for Research on Cancer; IATA - International Air Transport Association; IBC - International Bulk Chemical Code; IC50 - Half maximal inhibitory concentration; ICAO - International Civil Aviation Organization; IECSC - Inventory of Existing Chemical Substances in China; IMDG - International Maritime Dangerous Goods; IMO - International Maritime Organization; ISHL - Industrial Safety and Health Law (Japan); ISO - International Organization for Standardization; KECI - Korean Existing Chemicals Inventory; LC50 - Lethal Concentration 50%; LD50 - Lethal Dose 50%; MARPOL - International Convention for the Prevention of Pollution from Ships; Southern Common Market - Facilitation Agreement for the Transport of Dangerous Goods; n.o.s. - not otherwise specified; Nch - Chilean certification; NO(A)EC - No Observed Adverse Effect Concentration; NO(A)EL - No Observed Adverse Effect Level; NOELR - No Observed Effect Level of Exposure; NOM - Mexican Official Standard; NTP - National Toxicology Program; NZIoC - New Zealand Inventory of Chemicals; OECD - Organisation for Economic Co-operation and Development; OPPTS - Office of Pollution Prevention and Toxics; PBT - Persistent, Bioaccumulative and Toxic substance; PICCS - Philippine Inventory of Chemicals and Chemical Substances; (Q)SAR - (Quantitative) Structure-Activity Relationship; REACH - Registration, Evaluation, Authorisation and Restriction of Chemicals (EC) 1907/2006; SADT - Self-Accelerating Decomposition Temperature; SDS - Safety Data Sheet; TCSI - Taiwan Chemical Substance Inventory; TDG - Transport of Dangerous Goods; TECI - Thai Existing Chemicals Inventory; TSCA - Toxic Substances Control Act (USA); UN - United Nations; UNRTDG - United Nations Recommendations on the Transport of Dangerous Goods; vPvB - very Persistent and very Bioaccumulative substance; WHMIS - Workplace Hazardous Materials Information System.

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