Biosan CVP-2 Centrifuge Vortex for PCR Plates

1. About this edition of user instructions

The current edition of the user instructions applies to the following models:

Edition 3.02 – April of 2022

2. Safety Precautions

Caution! Make sure you have fully read and understood the present Manual before using the equipment. Please pay special attention to sections marked by this symbol.

2.1 Icons used on the unit and packaging

• CE: [CE Marking, manufacturer affirms conformity with European health, safety, and environmental protection standards, see 12.1]
• [WEEE Directive Marking, see 12.1]
• [Polarity of the power connector]
• [Equipment uses direct current]
• ⚠ [Do not use without lid. Secure the safety cover and the lid as described in 5.6]
• [Emergency opening location, see 5.18]

2.2 General safety

• This unit is a state-of-the-art piece of equipment, which is extremely safe to operate. Nevertheless, it may lead to danger for users or others nearby if used by untrained staff, in an inappropriate way or for purposes other than for those it was designed.
• Save the unit from shocks or falling.
• Store and transport the unit as described in section 10. Storage and transportation on page 14.
• Use only original accessories provided by the manufacturer and ordered specifically for this model.
• Before using any cleaning or decontamination methods except those recommended by the manufacturer, check with the manufacturer that the proposed method will not damage the equipment.
• Do not make modifications to the design of the unit.

2.3 Electrical safety

• Connect only to the external power supply with voltage corresponding to that on the serial number label.
• Use only the external power supply provided with this product.
• Ensure that the external power supply is easily accessible during use.
• Do not plug the unit into an ungrounded mains outlet, and do not use an ungrounded extension lead.
• Disconnect the unit from the electric circuit before moving.
• Disconnect the external power supply from mains outlet to turn off the unit.
• If liquid penetrates into the unit, disconnect it from the external power supply and have it checked by a repair and maintenance technician.
• Do not operate the unit in premises where condensation can form. Operating conditions of the unit are defined in section 7. Specifications on page 11.

2.4 During operation

• Do not use rotors with visible signs of corrosion, wear or mechanical damage.
• Do not use the unit without the rotor safety cover.
• Do not operate the unit in environments with aggressive or explosive chemical mixtures. Please contact manufacturer for possible operation of the unit in specific atmospheres.
• Observe the safety area of 300 mm around the unit. Persons and hazardous materials must not be located in the safety area whilst the unit is operating.
• Do not centrifuge flammable or chemically active substances.
• Do not use outside laboratory rooms.
• Do not operate the unit if it is faulty or was installed incorrectly.
• Always fix rotor securely. Stop the operation immediately with the Stop key if any unusual noise occurs during acceleration, which can be due to improper rotor fixation.

2.5 Biological safety

• According to EN 61010-2-020, a centrifuge without biological safety system is not considered a biologically safe and therefore cannot be used for centrifuging hazardous materials contaminated with toxic, radioactive or pathogenic microorganisms.
• Take additional measures if a contact of bare skin with hazardous substances is possible. Take care not to cut, puncture, or tear the gloves. Not one glove material is impervious to all substances. Disposable surgical or PVC gloves provide substantial but not complete protection. PVC gloves are probably more protective than surgical gloves, but they are stiffer and less tactile. Discard gloves after each use.
• It is the user's responsibility to carry out appropriate decontamination if hazardous material spills on or penetrates into the equipment.
• Some inner components are susceptible to contamination. Only qualified specialists, who are experienced in deactivations procedures, must perform cleaning these components for their future use.
• The user is responsible for decontaminate the unit before its decommissioning and utilization.

3. General Information

After many years of Combined Centrifuge/Vortex concept success, Biosan is proud to introduce the long-awaited Centrifuge/Vortex for PCR plates, CVP-2, to the sample preparation market. The application range of CVP-2 is PCR plates: with skirts, semi-skirts or without them.

The Spin-mix-spin technology is intended to spin-down micro volumes of reagents on the well's bottom (first centrifugation spin), following mixing (mix) and spin-down the reagents again (second spin). This repetitive algorithm, named SMS (spin-mix-spin), aims at reducing the mistakes during sample preparation for PCR analysis. The algorithm was published for the first time and patented by Biol. Dr. V. Bankovskis (V. Bankovskis et al., Riga, Latvia, Pat. No. P94-74).

CVP-2 is a fully automatic device for reproducing SMS for 2 PCR plates at the same time, thus saving time considerably. It is a must-have instrument for PCR and DNA analyses laboratory.

CVP-2 is 4 devices combined in 1:

1. Centrifuge – Maximum RCF: 175 × g (1500 rpm)
2. Vortex (regulation timer 0 – 60 s)
3. Centrifuge/Vortex
4. SMS for realization of the SMS algorithm

4. Getting started

4.1 Unpacking

Remove packing materials carefully and retain them for future shipment or storage of the unit. Examine the unit carefully for any damage incurred during transit. The warranty does not cover in-transit damage. Warranty covers only the units transported in the original package.

4.2 Standard set. Package contents:

• CVP-2 Centrifuge/Vortex: 1 pce
• R-2MP rotor for PCR plates with fixation nut for fastening and safety cover: 1 pce
• AP-96 adapter for semi-skirted and non-skirted PCR plates, 96 wells: 2 pcs
• Wrench for rotor removing: 1 pce
• Key for cover unblocking (fixed into rear panel of the unit): 1 pce
• External power supply: 1 pce
• Power cable: 1 pce
• User instructions, declaration of conformity: 1 copy

4.2.2 Optional accessories, on request

• AP-384 adapter 384-well PCR plates (Eppendorf): 2 pcs.

Image description: A wrench tool for rotor removal, the AP-96 adapter for 96-well PCR plates, and the AP-384 adapter for 384-well PCR plates.

4.3 Setup

• Place the unit on an even, stable, horizontal, and clean surface.
• Remove protective film from the display.
• Plug the power cable into the external power supply.
• Plug the external supply into the power socket on the rear of the unit and position the unit so that there is easy access to the power switch and mains outlet.
• According to EN 61010-2-20, people and hazardous materials must not be within a 300 mm area around the device during centrifuge operation.

4.4 Rotor installation

• Connect the power cord to a properly grounded mains outlet. Switch on the power switch (I position) on the rear.
• Press the [Open Button] and open the lid by lifting it upwards by hand.
• Unscrew the fixation nut (fig. 1/1) counter clockwise using the wrench included in the standard set and remove the nut.
• Place the rotor and secure it tightly with the fixation nut, placing the nut with the key holes up (fig. 1/1) and turning it clockwise by the included wrench.
• Place the safety cover (fig. 1/2) on the rotor by pressing the cover clip (fig. 1/3) down.
• Close the centrifuge lid.
• Note: To close the lid, press down the middle part of the edge of the lid until the clicking sound is heard. Otherwise, the lid will not be secured properly, which will cause operation malfunction.
• Turn off the centrifuge with the switch on the rear (position O).

Figure 1: Rotor replacement illustration showing the fixation nut, safety cover, and cover clip.

5. Operation

5.1 Recommendations during operation

• Do not use rotors with visible signs of corrosion, wear, or mechanical damage. Check the rotor and buckets for any signs of wear or corrosion, and replace if necessary.
• When using semi-skirted or non-skirted PCR plates, put the plate into an appropriate adapter. Insert the plate into the rotor with the adapter. Refer to section 6. How to choose a proper adapter.
• Load both PCR plates to balance the unit during operation.
• Before operation, check PCR plates and ensure that they are closed properly. Content can be spilled from open PCR plates, endangering personnel working with hazardous materials.
• For proper mixing, it is recommended to fill PCR plates not more than 75% of their nominal volume.
• Seal the microplates with an appropriate adhesive film to avoid spilling the sample.

Figure 2: Front panel illustration of the CVP-2 unit with labeled controls.

5.2 Power On

Connect the external power supply to a properly grounded power socket. Turn on the power switch (I position) on the rear side of the unit. The lid unlocks automatically.

5.3 Open Lid

Press the [Open Button] (fig. 2/1) and open the lid by lifting it upwards by hand (indication [Lid Open] will appear in the Mode bottom line, fig. 2/2). The lid will open only with the rotor stopped.

5.4 Remove Safety Cover

Remove the safety cover by lifting the safety cover clip.

5.5 Insert Plates

Insert TWO microtest plates in the rotor one opposite another.

5.6 Place Safety Cover and Close Lid

Place the safety cover on the rotor and secure it by pressing the safety cover clip down tightly. Close the outer lid carefully and smoothly until the clicking sound is heard (indication [Lid Closed] will appear in the Mode bottom line).

Note: To close the lid, press down the middle part of the edge of the lid until the clicking sound is heard. Otherwise, the lid will not be secured properly, which will cause operation malfunction.

5.7 Quick centrifugation

Press the [QS Button] (fig. 2/10) for quick mixing/sedimentation and hold it pressed for the desired time. The unit will automatically stop when the [QS Button] is released.

5.8 Setting parameters

• By pressing the [Select Button] (fig. 2/3), choose a parameter to change. Each pressing of the [Select Button] consecutively activates parameters in the operation cycle; the currently active parameter is indicated by blinking.
• Use the [Up Arrow] and [Down Arrow] keys (fig. 2/4) for setting the corresponding values. Pressing a key for longer than 2 seconds will increase the change rate.
• Speed (RPM) and spinning/mixing time (Time) can be changed during operation. When a new operation cycle starts, the microprocessor automatically accepts changes as the current operation mode. Cycle count (Cycle) and operating mode (Mode) cannot be changed during operation.
• Mode parameter (fig. 3/1) can be one of the following:
  • SMS: SMS-algorithm mode.
  • MS: Alternating centrifugation and vortexing.
  • SPIN: Centrifugation only.
  • MIX: Vortexing only.

5.9 SMS-algorithm

• Select the SMS-algorithm (indication S.M.S. of Mode parameter, fig. 3/6).
• Set the required centrifugation speed, from 300 to 1500 rpm (increment 100 rpm, fig. 3/2).
• Set the required centrifugation time, from 1 s to 30 min (increment 1 s, after 1 min – 1 min, fig. 3/1).
• Set the required vortexing speed, from 300 to 1200 rpm (increment 100 rpm, fig. 3/4).
• Set the required vortexing time, from 1 to 60 s (increment 1 s, fig. 3/3).
• Set the required SMS-algorithm cycle count, from 1 to 999 (fig. 3/5).

5.10 Centrifugation + vortexing (MS)

• Select the alternating centrifugation and vortexing algorithm (indication M.S. of Mode parameter, fig. 4/6).
• Set the required centrifugation speed, from 300 to 1500 rpm (increment 100 rpm, fig. 4/2).
• Set the required centrifugation time, from 1 s to 30 min (increment 1 s, after 1 min – 1 min, fig. 4/1).
• Set the required vortexing speed, from 300 to 1200 rpm (increment 100 rpm, fig. 4/4).
• Set the required vortexing time, from 1 to 60 s (increment 1 s, fig. 4/3).
• Set the required SMS-algorithm cycle count, from 1 to 999 (fig. 4/5).

5.11 Centrifugation (SPIN)

• Select the centrifugation mode (indication SPIN of Mode parameter, fig. 5/3).
• Set the required centrifugation speed, from 300 to 1500 rpm (increment 100 rpm, fig. 5/2).
• Set the required centrifugation time, from 1 s to 30 min (increment 1 s, after 1 min – 1 min, fig. 5/1).

5.12 Vortexing (MIX)

• Select the vortexing mode (indication MIX of Mode parameter, fig. 6/3).
• Set the required vortexing speed, from 300 to 1200 rpm (increment 100 rpm, fig. 6/2).
• Set the required vortexing time, from 1 to 60 s (increment 1 s, fig. 6/1).

Figure 3: SMS-algorithm display example.

Figure 4: Centrifugation + vortexing display example.

Figure 5: Centrifugation display example.

Figure 6: Vortexing display example.

5.13 Start Operation

Press the [Run Button] (fig. 2/5) to start operation.

5.14 Rotor Rotation

The rotor will begin rotating, and the display will show indications (fig. 2/2), cycle countdown (fig. 2/6), and changing parameters of the current mode (fig. 2/7 and fig. 2/8).

5.15 Stop Operation

Operation can be stopped before the set number of cycles elapses, if necessary, by pressing the [Stop Button] (fig. 2/9).

5.16 Automatic Stop

The unit stops automatically after executing the set number of cycles (blinking indication on display, fig. 2/2) and the lid unlocks automatically. A sound signal is emitted after the full stop of the rotor. Press the [Stop Button] (fig. 2/9) to stop the signal.

5.17 Power Off

At the end of operation, set the power switch to position O (OFF) on the rear. Disconnect the external power supply from the electric circuit.

5.18 Lid emergency opening

Note: The electrical lid lock allows opening the lid only when the unit is connected to the mains and is turned on.

Disconnect the power cord from the external power supply unit. Wait until the rotor stops completely. Insert the provided lid-unblocking key (found on the rear panel of the unit) into the marked opening on the right side of the unit and press to unlock the lid.

6. How to choose a proper adapter

The table below displays PCR plate types in relation to available adapters.

Caution! PCR plate height inside an adapter should not be bigger than 17 mm.

7. Specifications

The unit is designed for operation in cold rooms, incubators, and closed laboratory rooms at ambient temperatures from +4°C to +40°C in a non-condensing atmosphere and maximum relative humidity 80% for temperatures up to 31°C, decreasing linearly to 50% relative humidity at 40°C.

Biosan is committed to a continuous program of improvement and reserves the right to alter design and specifications of the equipment without additional notice.

• Speed setting range:
• Centrifugation: 300–1500 rpm
• Vortexing: 300–1200 rpm
• Speed setting increment: 100 rpm
• Relative centrifugal force: up to 175 × g
• Centrifugation mode time range: 1 s – 30 min
• Centrifugation mode time increment: 1 s; after 1 min – 1 min
• Vortex mode time range: 0–60 s
• Vortex mode time increment: 1 s
• Number of programmable cycles: 1–999 cycles
• Maximum plate height: 17 mm
• Display: LCD, 2x16 characters
• Safety measures: Safety cover, lid with a lock
• Dimensions: 285x350x190 mm
• Operating voltage and current: 12 V=, 1.5 A
• Power consumption: 18 W
• External power supply input: in 100–240V~, 50/60 Hz, out 12 V=
• Weight, accurate within ± 10%: 6.15 kg

8. Ordering information

8.1 Models and versions available

8.2 Optional accessories and replacement parts

To inquire about or order the optional accessories or the replacement parts, contact Biosan or your local Biosan representative.

8.2.1 Optional accessory

8.2.2 Replacement parts

9. Care and maintenance

9.1 Service

• If the unit is disabled (e.g., no centrifugation or vortexing, no reaction to key presses, etc.) or requires maintenance, disconnect the unit from the mains and contact Biosan or your local Biosan representative.
• All maintenance and repair operations (except listed below) must be performed only by qualified and specially trained personnel.
• Operating integrity check: If the unit follows the procedure described in section 5. Operation, then no additional checks are required.

9.2 Cleaning and disinfection

• Use mild soap and water with a soft cloth or sponge for cleaning the exterior. Rinse remaining washing solution with distilled water. Wipe dry the excess water with a clean, soft cloth or sponge.
• To disinfect the plastic parts, use 75% ethanol or DNA/RNA removing solution (e.g., Biosan PDS-250). After disinfecting, it is necessary to wipe the surfaces dry.
• To access the rotor, open the lid. For a powered unit, see 5.3. For an unpowered unit, see 5.18. Hold the rotor with one hand, unscrew the rotor fixation nut, turning it counter clockwise using the included wrench. Retrieve the rotor, clean, and disinfect, as necessary. Replace the rotor and secure it tightly with the fixation nut, placing the nut with the key holes up and turning it clockwise by the securing wrench. Replace the rotor safety cover on the rotor by pressing the cover clip down tightly.
• The rotor and adapters are autoclavable (15 min 121°C); the unit itself is not autoclavable.

9.3 Disposal

Disposal of the appliance requires special precautions and must be carried out at an appropriate disposal site, separate from normal household waste. To prevent pollution of the environment, all waste resulting from the disposal of the product must be collected and disposed of in the country of use, in accordance with the applicable requirements for the handling of electronic waste.

10. Storage and transportation

• Store and transport the unit in a horizontal position (see package label) at ambient temperatures between -20°C and +60°C and maximum relative humidity of 80%.
• After transportation or storage and before connecting it to the electric circuit, keep the unit under room temperature for 2-3 hrs.
• For extended storage, the unit does not require special procedures.

11. Warranty. Production date

• The Manufacturer guarantees the compliance of the unit with the requirements of Specifications, provided the Customer follows the operation, storage, and transportation instructions.
• The warranted service life of the unit from the date of delivery to the Customer is 24 months (excluding optional parts mentioned in the table in the Specifications section). For extended warranty, register the unit, see 11.5.
• Warranty covers only the units transported in the original package.
• If any manufacturing defects are discovered by the Customer, an unsatisfactory equipment claim shall be compiled, certified, and sent to the local distributor address. To obtain the claim form, visit the Technical support section on our website at the link below.
• Extended warranty: For CVP-2, a Premium class model, one year of extended warranty is available free of charge after registration, during 6 months from the date of sale. Online registration form can be found in the Warranty registration section on our website at the link below.
• Description of the classes of our products is available in the Product class description section on our website at the link below.

Technical support: https://biosan.lv/en/support

Warranty registration: https://biosan.lv/register-en

Product class description: https://biosan.lv/classes-en

11.7 Information required for service

The following information will be required in the event that warranty or post-warranty service becomes necessary. Complete the table below and retain for your records.

11.8 Production date

Production date is placed in the serial number, on the label of the unit. The serial number consists of 14 digits styled XXXXXXYYMMZZZZ, where XXXXXX is the model code, YY and MM – year and month of production, ZZZZ – unit number.

12. EU Declaration of conformity

Centrifuge-vortex for PCR plates CVP-2 is in conformity with the following relevant Union legislations:

• LVD 2014/35/EU: LVS EN 61010-1:2011 Safety requirements for electrical equipment for measurement, control, and laboratory use. General requirements. LVS EN 61010-2-020:2016 Particular requirements for laboratory centrifuges.
• EMC 2014/30/EU: LVS EN 61326-1:2013 Electrical equipment for measurement, control and laboratory use. EMC requirements. General requirements.
• RoHS3 2015/863/EU: Directive on the restriction of the use of certain hazardous substances in electrical and electronic equipment.
• WEEE 2012/19/EU: Directive on waste electrical and electronic equipment.

Declaration of Conformity is available for download on the page for the relevant model on our website by links below, in the Downloads section.

QR code for Declaration of Conformity: [Link to Downloads Section]