BACTRON BACTRON900-2 SHEL LAB Anaerobic Chamber Instruction Manual

Instruction Manual for BACTRON models including: BAA90022, BAA90022-E, BACTRON900-2 SHEL LAB Anaerobic Chamber, BACTRON900-2, SHEL LAB Anaerobic Chamber, LAB Anaerobic Chamber, Anaerobic Chamber, Chamber

BACTRON900 BACTRON900-2

BACTRON900

Audrey Sonka

/e85326dbe035dd6bcf72738737dd9523e2d0aea3c20898f62...

BACTRON900 BACTRON900-2

File Info : application/pdf, 98 Pages, 4.55MB

4861873 UMAN BACTRON900 and 900-2

Installation - Operation Manual
BACTRON900, BACTRON900-2
110 120 Volts 2210| P a2g40e Volts

BACTRON900 Front View
Warning: This product contains chemicals, including Triglycidyl Isocyanurate, known to the State of California to cause cancer as well as birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov. ¡Advertencia! Este producto contiene sustancias químicas, incluido el triglicidil isocianurato, que el estado de California sabe que causa cáncer, así como defectos de nacimiento u otros daños reproductivos. Para obtener más nformación, visite www.P65Warnings.ca.gov. Avertissement! Ce produit peut vous exposer à des produits chimiques, dont l'isocyanurate de triglycidyle, reconnu par l'État de Californie pour provoquer le cancer, des anomalies congénitales ou d'autres problèmes de reproduction. Pour plus d'informations, visitez le site www.P65Warnings.ca.gov.
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BACTRON900 Anaerobic Chamber 110 120 Volts 220 240 Volts

Part Number (Manual): 4861873 Revision: December 03, 2024

Sheldon Part ID Numbers:

Model

BACTRON900 (110V)

BACTRON900-2 (220 V)

Part ID

BAA90022

BAA90022-E

The Part ID denotes the specific build type of the model.

Shellab is a brand of Sheldon Manufacturing, INC, an ISO 9001 certified manufacturer.
Safety Certifications

EN 61010-1:2010/A1:2019 EN IEC 61010-2-010:2020 UL 61010-1:2012/R:2023-06 UL 61010-2-010:2019 CSA C22.2 No. 61010-1:2012/U3:2023-06 CSA C22.2 No. 61010-2-010:2019

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TABLE OF CONTENTS
Introduction ................................................................................................................................ 7
Read this Manual.............................................................................................................................. 7 Safety Considerations and Requirements ........................................................................................... 7 Contact Assistance ........................................................................................................................... 9 Engineering Improvements ............................................................................................................... 9 Manufacturing Defect Warranty ......................................................................................................... 9 Reference Sensor Device ................................................................................................................ 13
RECEIVING YOUR BACTRON900 ............................................................................................... 15
Inspect the Shipment ..................................................................................................................... 15 Orientation..................................................................................................................................... 17 Record Data Plate Information ........................................................................................................ 19
INSTALLATION .......................................................................................................................... 21
Installation Checklist....................................................................................................................... 21 Required Ambient Conditions .......................................................................................................... 22 Sufficient Workspace ...................................................................................................................... 22 Environmental Disruption Sources ................................................................................................... 22 Eliminate UV Lighting...................................................................................................................... 23 Power Requirements....................................................................................................................... 23 Gas Supply Requirements ............................................................................................................... 24 Lifting and Handling ....................................................................................................................... 26 Leveling ......................................................................................................................................... 26 Installing the BACTRON900............................................................................................................. 26 Attach the Regulator to the Gas Supply Cylinder............................................................................... 27 Connect to the Gas Supply .............................................................................................................. 27 Connect the Foot Pedal Switch ........................................................................................................ 28 Fill the Manometer ......................................................................................................................... 28 Vacuum Supply .............................................................................................................................. 29 Installation Cleaning and Disinfection .............................................................................................. 29 Place Anaerobic Monitoring Strips .................................................................................................... 30 Install Incubator Shelf Spacers ........................................................................................................ 30 Open the Incubator Doors .............................................................................................................. 30 Close the Airlock Doors ................................................................................................................... 31 Installing the Armport Doors ........................................................................................................... 31
GRAPHIC SYMBOLS ................................................................................................................... 33
CONTROL PANEL OVERVIEW .................................................................................................... 35
Main Panel Incubator Controls......................................................................................................... 35 Shadowbox Control Panel ............................................................................................................... 38 BACTRON900 WORKSPACE INCUBATOR .......................................................................................... 39
OPERATION ............................................................................................................................... 41
Theory of Operation ....................................................................................................................... 41 Put the BACTRON900 into Operation ............................................................................................... 44 Install an O2 Scrubber Cartridge ...................................................................................................... 47 Plug In the BACTRON900................................................................................................................ 47 Supply Gas to the BACTRON900 ...................................................................................................... 48 Zero the Pressure Displays .............................................................................................................. 49 Launch the Anaerobic Commissioning Cycle ..................................................................................... 50 Attach the Sleeves ........................................................................................................................ 51 Enter the Chamber ......................................................................................................................... 52 Moving in the Pressurized Chamber ................................................................................................. 54 Vacuuming Down Ballooning Sleeves ............................................................................................... 55 Anaerobic Monitoring Strips ............................................................................................................ 55 Verify the Anaerobic Atmosphere..................................................................................................... 56
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TABLE OF CONTENTS
Exit the Chamber............................................................................................................................ 57 Set the Incubator Temperature Setpoint .......................................................................................... 58 Set the Over Temperature Limit ...................................................................................................... 59 Set the Airlock Cycle Iterations ........................................................................................................ 60 Cycling the Airlock .......................................................................................................................... 60 Manually Cycling the Airlock ............................................................................................................ 61 Airlock Safety Aborts ...................................................................................................................... 62 Vacuum Pump Cooldown Lockout .................................................................................................... 62 Inner Airlock Door Lock .................................................................................................................. 62 Loading Samples ............................................................................................................................ 63 Humidifying the Incubators ............................................................................................................. 64 Chamber Accessory Power Outlets................................................................................................... 64 Volatile Compound Scrubber and Rejuvenation Cycle ........................................................................ 64 Condensation and the Dew Point ..................................................................................................... 65 Deionized and Distilled Water.......................................................................................................... 66 Pressure Unit Conversion Chart ....................................................................................................... 66
USER MAINTENANCE................................................................................................................. 67
Daily Maintenance .......................................................................................................................... 68 Normal Gas Consumption................................................................................................................ 68 Troubleshooting Persistent Oxygen in the Chamber .......................................................................... 69 Leak Diagnostic Unit in Use Procedure .......................................................................................... 70 Leak Check Unit Empty ................................................................................................................ 72 Door Gasket Maintenance and Usage............................................................................................... 73 Sleeve Maintenance and Usage ....................................................................................................... 73 O2 Scrubber Cartridge: Test in the Chamber..................................................................................... 74 Reactivating O2 Scrubber Cartridges ................................................................................................ 74 Quality Control Test Scrubber Cartridges....................................................................................... 75 AMG Conservation Methods............................................................................................................. 75 Replacing Sleeve Components ......................................................................................................... 76 Cleaning and Disinfecting................................................................................................................ 77 Maintaining the Acrylic Glass Panels ................................................................................................ 79 Electrical Components .................................................................................................................... 79 Calibrate the Temperature Display................................................................................................... 80
UNIT SPECIFICATIONS ............................................................................................................. 85
Power ............................................................................................................................................ 85 Unit Weight.................................................................................................................................... 85 Shipping Weight ............................................................................................................................. 85 Unit Dimensions ............................................................................................................................. 85 Shelf Dimensions ............................................................................................................................ 86 Airlock Dimensions ......................................................................................................................... 86 Volumes and Capacity..................................................................................................................... 86 Temperature .................................................................................................................................. 87 Oxygen .......................................................................................................................................... 87
PARTS LIST................................................................................................................................ 88
Ordering Parts and Consumables..................................................................................................... 90
ACCESSORIES............................................................................................................................ 91
APPENDICES ............................................................................................................................. 95
AMG Usage .................................................................................................................................... 95 PLC Inputs and Outputs .................................................................................................................. 96
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INTRODUCTION
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INTRODUCTION
Thank you for purchasing a BACTRON900 Anaerobic Chamber. We know you have many choices in today's competitive marketplace when it comes to anaerobic cultivation systems. We appreciate you choosing ours. We stand behind our products and will be here for you if you need us.
READ THIS MANUAL
Failure to follow the guidelines and instructions in this user manual may create a protection impairment by disabling or interfering with the unit safety features. This can result in injury or death. Before using the unit, read the manual in its entirety to understand how to install, operate, and maintain the unit in a safe manner. Ensure all operators are given appropriate training before the unit begins service. Keep this manual available for use by all operators.
SAFETY CONSIDERATIONS AND REQUIREMENTS
Follow basic safety precautions, including all national laws, regulations, and local ordinances in your area regarding the use of this unit. If you have any questions about local requirements, please contact the appropriate agencies.
SOPs Because of the range of potential applications this unit can be used for, the operator or their supervisors must draw up a site-specific standard operating procedure (SOP) covering each application and associated safety guidelines. This SOP must be written and available to all operators in a language they understand.
Intended Applications and Locations BACTRON900 Anaerobic chambers are intended for professional, industrial, and educational applications suitable for the cultivation of clinical bacteria. These units are not intended for use at hazardous or household locations. Use this equipment only for its intended range of applications.
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INTRODUCTION

Power

Your unit and its recommended accessories are designed and tested to meet strict safety requirements.

· The unit is designed to connect to a power source using the specific power cord type shipped with the unit.

· Always plug the unit's power cord into a protective earth-grounded electrical outlet that conforms to national and local electrical codes. If the unit is not grounded properly, parts
such as knobs and controls can conduct electricity and cause serious injury.

· Do not bend the power cord excessively, step on it, or place heavy objects on it.

· A damaged cord can be a shock or fire hazard. Never use a power cord if it is

damaged or altered in any way.

Shock or

· Use only approved accessories. Do not modify system components. Any alterations Fire Hazard

or modifications to your unit not explicitly authorized by the manufacturer can be dangerous

and will void your warranty.

BACTRON900
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INTRODUCTION
CONTACT ASSISTANCE
Phone hours for Support are 6 am 4:30 pm Pacific Coast Time (west coast of the United States, UTC-8), Monday Friday. Please have the following information ready when calling or emailing Technical Support: the model number and the serial number. These will be found on the unit data plate located in the workspace chamber above the inner airlock door. See 19. support@sheldonmfg.com 800-322-4897 extension 4 503-640-3000 extension 4
Sheldon Manufacturing, INC. P.O. Box 627 Cornelius, OR 97113 USA
ENGINEERING IMPROVEMENTS
Sheldon Manufacturing continually improves its products. As a result, engineering changes and improvements are made from time to time. Therefore, some changes, modifications, and improvements may not be covered in this manual. If your unit's operating characteristics or appearance differ from those described in this manual, please contact your BACTRON900 dealer or distributor for assistance.
MANUFACTURING DEFECT WARRANTY
For information on your warranty and online warranty registration please visit: · sheldonmanufacturing.com/warranty
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INTRODUCTION
AMG Supply
The BACTRON900 requires a continual supply of Anaerobic Mixed Gas to establish and maintain an anaerobic atmosphere. The gas mix must have 5% hydrogen to drive the BACTRON900 catalytic oxygen scrubbing process. The manufacturer recommends an AMG mixture ratio of 5% hydrogen, 5% carbon dioxide, and 90% nitrogen. Note: Do Not exceed 5% hydrogen concentration or explosive mixtures can occur.
The BACTRON900 can be connected to either a standalone supply cylinder or an in-house system. Please see page 24 for more information. On-Site Supply The manufacturer strongly recommends keeping at least two size 200 cylinders of AMG (size N 8.76M3) on hand or a house supply system equivalent to ensure a continual supply of AMG. Anticipated Gas Usage AMG usage in the BACTRON900 is highly variable. Consumption is primarily driven by the following factors:
· The number of times the chamber is accessed each day. o Airlock and armport sleeve cycles consume AMG.
· The amount of time laboratory personnel spend working with their arms in the sealed workspace chamber. o Movement displaces the chamber atmosphere, some of which is vented and must be replaced with AMG injections.
· Laboratory personnel should adhere as close as possible to the proper movement technique guidelines while working in the chamber.
Page 95 contains AMG usage data for specific BACTRON900 operations. Airlock cycles may be supplemented with nitrogen to help reduce AMG use. Please see pages 24 and 25 for additional gas requirement information.
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INTRODUCTION

Required Gas Pressure Delivery to the BACTRON900

Delivery of less than 50 psi gas flow pressure to the BACTRON900 may slow cycle times. Delivery pressures of less than 40 psi will interrupt the airlock, sleeve, and commissioning cycles, and prevent the BACTRON900 from maintaining overpressure in the workspace chamber.

Factors that can reduce gas pressure delivery include:

· The total volume of the delivery system, including:

o The distance between the BACTRON900 and the supply source.

o Incorrectly sized gas tubing.

· The total number of units attached and drawing from a building gas supply system.

· Incorrect regulator settings.

Gas regulators may be set higher than 50 psi gas flow to overcome factors lowering the pressure in the supply system, if necessary. Never exceed a setting of 60 psi.

The manufacturer recommends waiting to introduce electronic devices into the workspace chamber until an anaerobic atmosphere has been established. Condensation may take place in the chamber during the anaerobic commissioning cycle.

Open the incubator door.
The incubator door must be open during the commissioning cycle while the BACTRON900 establishes an anaerobic atmosphere in its workspace chamber.

Open the Incubator Door

· Failure to open the incubator door will leave a significant reservoir of oxygenated atmosphere in the incubator.

· The airlock doors must be closed and latched prior to launching a commissioning cycle. The inner door will lock when the BACTRON900 is turned On.

· The armport doors must be installed for the commissioning cycle to successfully create an anaerobic atmosphere.

Armport Doors Must be Installed during the Commissioning Cycle

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INTRODUCTION
Scrubber Cartridge Oven
The BACTRON900 requires a scrubber cartridge oven to reactivate the O2/activated carbon media scrubber cartridges. Each cartridge requires a bake out of at least 8 hours at 200°C to reactivate after 24-hours of use in the chamber. This necessitates at least one bake-out per day, with one scrubber mounted in the BACTRON900 workspace chamber while the other is being baked out.
Glassware
A glassware beaker or flask is placed under the drain tube for the condensatecapturing chiller plate in the chamber. The manometer pressure valve/gauge in the workspace chamber requires periodic water refills. Water is added by pouring or injecting water into the fill port on the top of the manometer body. Using a beaker or other glassware for transporting and pouring water into the manometer. Two 500 ml beakers or a container fan will be needed if you intend to use activated carbon media to scrub volatile fatty and sulfur compounds out of the workspace chamber atmosphere.
Oxygen Detection Strips
The BACTRON900 uses a minimum of 1 Oxoid brand oxygen-detecting anaerobic indicator strip every 24 hours to provide continual anaerobic surveillance in the workspace chamber. Part Number 9900706, 1 box of 100 strips. Each BACTRON900 comes with five oxygen detection strips. Five strips are sufficient to verify that an anaerobic atmosphere has been established in the workspace chamber.
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INTRODUCTION

REFERENCE SENSOR DEVICE
Must be purchased separately.

A reference sensor device is required for calibrating BACTRON900 incubator temperature displays. Reference devices must meet the following standards:
· Accurate to at least 0.1°C The device should be regularly calibrated, preferably by a third party.

Temperature Reference

Temperature Probe
Use a digital device with a wire thermocouple probe that can be introduced into the incubator through the incubator door space. Select thermocouples suitable for the application temperature at which you will be calibrating.

Why Probes?
Reference readings taken outside an incubator using a wire temperature probe avoids incubation chamber door openings. Openings disrupt the chamber temperature. Each disruption requires a minimum 1-hour wait to allow the temperature to re-stabilize before continuing. Always use thermocouple probes.

No Alcohol or Mercury Thermometers
Alcohol thermometers do not have sufficient accuracy to conduct accurate temperature calibrations. Never place a mercury thermometer in an incubator!

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INTRODUCTION
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RECEIVING YOUR BACTRON900

INSPECT THE SHIPMENT
1. When a unit leaves the factory, its safe delivery becomes the responsibility of the carrier. 2. Damage sustained during transit is not covered by the manufacturing defect warranty. 3. Save the shipping carton until you are certain the unit and its accessories function properly. When you receive your unit, inspect it for concealed loss or damage to its interior and exterior. If you find any damage to the unit, follow the carrier's procedure for claiming damage or loss.

1. Carefully inspect the shipping carton and report any damage to the carrier service that delivered the unit.

2. If the carton is not damaged, open the carton and remove the contents. 3. The unit should come with an end-user Installation and Operation. 4. Verify that the correct number of accessory items have been included.

See next page for required & useful accessories items.

Standard accessory items included with the BACTRON900 and BACTRON900-2:

Anaerobic Monitoring Strips (5 packets)

Armport Door Left

Armport Door Right

Shelf Spacers (3)

Do not open now!

O2 Scrubber Cartridges (2)

Foot Switch Control

Power Cords NEMA 5-15P
(2)

Leveling Feet (4)

Leave in the package! Do not
unwrap now.
Medium Sleeve Assemblies (2)
(Size 8)

Small Sleeve Cuffs (2) (Size 7)

Armport Door Stands (2)

Vacuum Supply: The BACTRON900 comes with an internal vacuum pump.

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RECEIVING YOUR BACTRON900

Required and Useful Accessories
1. An AMG regulator and a second O2 scrubber are required to operate the BACTRON900.
2. The unit comes with an LED Light Unit 220V and 2 X 11 Petri Dish Racks; these components can be ordered separately.

O2 Scrubber Cartridge

LED Light Unit 220V

Petri Dish Rack (2 X 11 Plates)

9990759 Required!

9730519 Useful

5110729 Useful

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RECEIVING YOUR BACTRON900 ORIENTATION
Figure 1: BACTRON900 / BACTRON900-2
Figure 2: Workspace Chamber Layout
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RECEIVING YOUR BACTRON 300
Workspace Chamber Orientation
BACTRON900
Figure 3: Power Supply Panel (Located on back of unit, behind the Airlock)
Foot Switch Figure 3: Power Supply Panel
(Located on back of unit, behind the Airlock) Airlock Orientation & Data Plate
Figure 4: Airlock Inner Door, Sliding Shelf, and Data Plate The airlock has a sliding shelf that can transport up to 216 plates at a time.
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RECEIVING YOUR BACTRON900 RECORD DATA PLATE INFORMATION
The data plate contains the incubator model number, serial number, part number, and Part ID number. Tech Support will need this information during any support call. Record it below for future reference.
· The data plate is located in the workspace chamber above the inner airlock door. Data Plate Information
Model No: Serial No: Part No: Part ID:
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INSTALLATION
INSTALLATION CHECKLIST
Pre-Installation:
Check that sufficient countertop space is available for the BACTRON900. Rolling stands to
mount the BACTRON900 on are available for purchase. Please see pages 22 and 91.
Check that the ambient conditions and ventilation spacing requirements are met, page 22. Check for sources of temperature and atmospheric disruption in the environment, page 22. Verify that no damaging UV light sources are present, page 23. Check that a suitable electrical outlet is present, page 23. Procure an AMG gas supply for the BACTRON900 suitable for your application. See page 24 for
gas requirements. Optional: Obtain a nitrogen (N2) supply to reduce AMG usage during airlock cycles. Obtain an N2 regulator and tubing. Please see pages 24 and 93.
Install the BACTRON900 in a suitable location:
Review lifting and handling instructions, page 26. Make sure the BACTRON900 is level, page 26.
Set up the BACTRON900 for use:
Connect the gas supply source(s) to the BACTRON900, page 27. Connect the foot pedal switch to the BACTRON900, page 28. Fill the manometer in the workspace chamber with water, page 28. Clean and disinfect the BACTRON900, accessories, and items that will be placed in the
chamber, page 29.
Place the listed BACTRON900 accessories in the chamber, page 30. Place 5 unopened anaerobic monitoring packets or strips in the workspace chamber, page 30. Install shelf spacers in the workspace incubator (BACTRON900), page 30. Open the incubator doors open (centered), page 30. Close and latch both airlock doors, page 31. Install the armport doors, page 31.
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INSTALLATION

REQUIRED AMBIENT CONDITIONS
BACTRON900s are intended for use indoors at ambient room temperatures between +15C to 30C (59F and 86F), at no greater than 80% Relative Humidity (at 25C / 77F). The room temperature should not vary more than +/-1°C per hour.
Operating the BACTRON900 outside of these conditions may adversely affect its incubator temperature stability and effective operating range. For conditions outside of those listed above, please contact your BACTRON900 distributor to explore other options suited to your laboratory or production environment.

SUFFICIENT WORKSPACE
2.5 inches (64mm) minimum clearances required for unobstructed airflow and cooling:

BACTRON900 - 2.5" (64mm) Gas Connections
2.5" (64mm)

2.5" (64mm)

2.5" (64mm) Power Cord
2.5" (64mm)

Airlock Door Swing

· Gas Connections: The BACTRON900 requires a continual connection to 1 or 2 compressed gas sources. Ensure there is sufficient space to access these connections.
· A control panel is located in the shadow box on the left side of the unit, behind a sliding door. Ensure users have sufficient room to access the panel.
· Please see page 85 for unit dimensions. Caster-mounted stands for BACTRON900s are available for purchase.
ENVIRONMENTAL DISRUPTION SOURCES
Consider proximate environmental factors that can affect the chamber temperature and atmospheric integrity when selecting a location to install the BACTRON900:
· Ovens, autoclaves, and any device that produces significant radiant heat · High-traffic areas · Direct sunlight · Heating and cooling ducts, or other sources of fast-moving air currents Direct exposure to air-conditioning vents or other sources of cold air can result in condensation or fogging on the acrylic glass panels of the chamber, depending on humidity and other ambient conditions. Prolonged exposure to cold air flows may adversely affect the incubator temperature performance.
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INSTALLATION
ELIMINATE UV LIGHTING
Sustained exposure to direct sunlight, UVC, or UV germicidal lighting around 254nm will cause rapid aging of the BACTRON900 acrylic glass panels and armport sleeves. Check to see if your laboratory or workspace contains sources of UV lighting.
Periodic use of long-wave (365nm) UV hand lamps for bacterial identification should not damage the acrylic glass. See the Maintaining the Acrylic Glass Panels entry on page 79 for more details.

POWER REQUIREMENTS
When selecting a location for the BACTRON900, check that each of the following requirements is satisfied.

Power Source: The power source for the BACTRON900 or BACTRON900-2 must match the voltage and match or exceed the ampere requirements listed on the unit data plate. BACTRON900s are intended for 110 - 120V and 220 240V, 50/60 Hz applications at the following amperages:

Model
BACTRON900 BACTRON900-2

Voltage
110-120 220-240

Amperage
14 Amps 10 Amps

Standard NEMA 5-15P wall socket

· The unit may be damaged if the supplied voltage varies by more than 10% from the data plate rating.
· The wall power source must be protective earth-grounded.
· Use a separate circuit to prevent loss of the unit due to overloading or circuit failure.
· The recommended wall circuit breakers for these units are 15 amps.
· The wall power source must conform to all national and local electrical codes.
Power Cord and Fuses: The unit must be positioned so that all end-users can quickly unplug the BACTRON900 in the event of an emergency.
· The unit comes with two 15 Amp, 125V, 9 ft. 5 in. (2.86m) NEMA 5-15P power cords. Always use these cords or identical replacements.
· These units come with one T12.5 Amp, 250V 5x20mm fuse located in the power cord inlet. The unit must be fused to operate.
· These units come with one T16.0 Amp, 250V 5x20mm fuse located in the secondary inlet that is wired to the interior outlet. The unit must be fused to operate.
Vacuum Pump: These units each come with an internally mounted vacuum pump.
· The BACTRON900 and BACTRON900-2 have one pair of T10 Amp, 250V 5x20mm fuses installed in a fuse holder on the power connection panel on the back of the unit. The fuses must be installed and unblown for the pump to operate.

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INSTALLATION
GAS SUPPLY REQUIREMENTS
Warning: Never exceed a 5% hydrogen concentration inside the anaerobic workspace chamber. Exceeding 5% creates an explosion and flammability hazard. Avertissement: La concentration d'hydrogène ne doit pas dépasser 5% dans la chambre anaérobie. Un dépassement de 5% crée un risque d'explosion et d'inflammabilité.

AMG (Anaerobic Mixed Gas) Required
A supply source of AMG sufficient to conduct the Anaerobic Commissioning Cycle and operate the unit afterward must be on hand prior to placing the BACTRON900 into operation. The Manufacturer strongly recommends keeping a second AMG cylinder on site to ensure a continual supply of AMG.
Anaerobic Mixed Gas is often sold by gas suppliers under the category of Anaerobic Incubation Mixtures or Biological Atmospheres. Laboratories that are required to be compliant with Good Laboratory Practices may require batch-certified AMG.
Reminder: The recommended BACTRON900 AMG mix is 5% H2, 5% CO2, 90% N2.

Airgas Part Numbers for AMG: H2 5%, CO2 5%, N2 90%, Size 200, CGA 350:
Z03NI9022000008 Standard
Z03NI9032000041 Analyzed with Certificate.

Contact your site safety officer and review your institutional safety protocols for handling, storing, and using compressed gases. Follow all local ordinances and national regulations regarding compressed gases in research, clinical, or production environments.

AMG Regulator Requirements

An AMG Regulator is required to operate the BACTRON900.

The AMG Regulator provided by Sheldon Manufacturing with the BACTRON900 is compliant with the requirements listed below:

· The regulator for each AMG cylinder must be a dual-stage regulator to ensure precise flow rates.

· The AMG regulator must be rated for hydrogen.

· Must be capable of delivering 50 psi of gas flow to the BACTRON900 (345kPa).

· The supply tubing from the gas regulator to the BACTRON900 must be 3/16-inch ID

(inside dimension).

Supply Pressure

Flow Setting

Gauge

Pressure Gauge

AMG Regulator

PN: 7150511

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INSTALLATION
Nitrogen Option - Dual Gas Configuration
AMG is used to cycle the airlock to create and maintain an anaerobic atmosphere in the workspace chamber. This is a major source of AMG usage; however, AMG is only necessary for the final gas backfill. To reduce AMG consumption, connect a nitrogen (N2) supply to the BACTRON900 N2 In gas port. The BACTRON900 will draw from the N2 In gas port during gas backfills for every iteration of an airlock auto cycle, except the final iteration. The unit must be connected to both an AMG supply source (AMG In port) and a N2 source for the dual gas configuration to function. See illustrations on page 27.
· For dual gas configurations, the manufacturer recommends a cylinder of AMG, along with a cylinder of 100% Nitrogen (N2).
· The nitrogen must be medical or food grade. The use of industrial-grade nitrogen risks introducing impurities into the workspace chamber and damaging components.
· The nitrogen regulator must be a dual-stage regulator rated for nitrogen, connected to 3/16" ID gas tubing, and be capable of delivering 50 psi of gas flow to the BACTRON900 (345kPa).
· The BACTRON900 will not draw from the nitrogen supply during manual airlock cycles or when cycling the armport sleeves.
Required Gas Pressure Delivery to the BACTRON900
Delivery to the BACTRON900 of less than 50 psi gas flow pressure may slow cycle times. Delivery pressure less than 40 psi will interrupt airlock, sleeve, and commissioning cycles, and prevent the BACTRON900 from maintaining overpressure in the workspace chamber. Factors that can reduce gas pressure delivery include:
· The total volume of the delivery system, including: o The distance between the BACTRON900 and the supply source. o Incorrectly sized gas tubing.
· The total number of units attached and drawing from a building gas supply system. · Incorrect regulator settings. If necessary, gas regulators may set higher than 50 psi gas flow to overcome factors lowering the pressure in the supply system. Never exceed a gas flow setting of 60 psi.
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INSTALLATION
LIFTING AND HANDLING
The BACTRON900 is heavy. Use appropriate powered lifting devices. Follow these guidelines when lifting and handling the BACTRON900:
· Lift the BACTRON900 only from its bottom surface. o Doors, handles, and knobs are not adequate for lifting or stabilization.
· Restrain the BACTRON900 completely while lifting or transporting to prevent tipping. · Remove all removable components, such as shelf spacers and trays, and secure all doors in
the closed position during transport and transfer to prevent shifting and damage. Note: To prevent damage when moving the BACTRON900, turn each of the four leveling feet
completely clockwise.
LEVELING
The BACTRON900 must be level and stable for safe operation. 1. Install the leveling feet included with the BACTRON900.
INSTALLING THE BACTRON900
Install the unit in a workspace location that meets the criteria discussed in the previous entries in the Installation section. Do not connect the unit to its power source at this time.
· Adjust the leveling feet until the chamber stands level and solid without rocking in its workspace location.
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INSTALLATION

ATTACH THE REGULATOR TO THE GAS SUPPLY CYLINDER
Note: Skip this procedure if the BACTRON900 will be drawing AMG from a building supply system.

Optional: Attach a nitrogen regulator to a nitrogen supply cylinder now if you will be using the dual-gas configuration.
CONNECT TO THE GAS SUPPLY
Warning: Never exceed a 5% hydrogen concentration inside the anaerobic workspace chamber. Exceeding 5% creates an explosion and flammability hazard. Avertissement: La concentration d'hydrogène ne doit pas dépasser 5% dans la chambre anaérobie. Un dépassement de 5% crée un risque d'explosion et d'inflammabilité.
Single Gas Configuration

AMG

T-Fitting

The T-Fitting Adaptor comes with the BACTRON900 Single Gas Configuration
Failure to connect the T-fitting to both the AMG and Nitrogen IN ports will interfere with the airlock auto cycle. The Nitrogen IN port must always be connected to either the AMG or an N2 supply. Dual Gas Configuration
AMG
N2

DO NOT start a flow of gas to the BACTRON900 at this time for either configuration.
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INSTALLATION
CONNECT THE FOOT PEDAL SWITCH
The foot pedal switch cycles the armports and attached sleeves. Connect the foot pedal cable to the two-pin female connection on the power access panel on the back of the BACTRON900.

FILL THE MANOMETER
The manometer acts as a pressure relief check valve and as a visual gauge of atmospheric pressure inside the sealed workspace chamber.
The manometer must be filled with 2 fluid ounces (60 ml). Failure to do so will compromise the chamber anaerobic atmosphere.

2 fl oz (60ml)

· Detach the reservoir bottle from the manometer assembly and remove from
unit for filling, remove the reservoir by rotating the bottle counter-clockwise.

NOTE: Due to risk of spilling, reservoir MUST be filled outside of the unit
· Underfilling or overfilling compromises the manometer accuracy as a pressure gauge and as a check valve. Fill with 2 fl oz (60 ml) of water.

Measuring Rings
Bottle

· The water should reach the top (black) measuring ring when the BACTRON-J is off and the bottom poppet ring when on.

· To avoid scaling (mineralization build-up), use distilled water. Never use deionized water.

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INSTALLATION
VACUUM SUPPLY
The BACTRON900 comes with an integrated vacuum pump which eliminates the need for vacuum pump connections during installation. The vacuum pump is used to partially evacuate the airlock and the armport sleeves when cycling to remove oxygen. The BACTRON900 is not designed to connect to an in-house vacuum supply system.
INSTALLATION CLEANING AND DISINFECTION
Clean and disinfect the chamber during installation to reduce the chance of microbiological contamination. The BACTRON900 was cleaned and the workspace chamber disinfected at the factory. However, the BACTRON900 may have been exposed to contaminants during shipping, and/or the factory procedure may not meet the standards of your institutional protocols.
Please see the Cleaning and Disinfecting entry on pages 77 and 78 in the User Maintenance section for information on how to clean and disinfect the unit without damaging the chamber.
Never use deionized water to clean or rinse the BACTRON900!
1. Remove all protective wrappings from accessories and the unit prior to cleaning/disinfecting. 2. Clean and disinfect the workspace chamber and incubator(s). 3. Clean, disinfect, and place the following accessories in the workspace chamber:
o The armport doors o The incubator bottom shelf spacers o The petri dish rack 2x11
The petri dish rack can be placed on the top shelf of the workspace chamber incubator or the BACTRON900 workspace shelves during the setup.
o A glass flask or beaker. When clean, place the glass flask or beaker under the plastic condensation tube on the left side of the chamber.
o You may place water-resistant, aerobic-tolerant items into the workspace chamber now. Doing so saves time and AMG usage by eliminating future airlock cycles.
The manufacturer recommends waiting to introduce electronic devices into the workspace chamber until after an anaerobic atmosphere has been established. Condensation may take place in the chamber during the anaerobic commissioning cycle.
Leave the O2 scrubber cartridge in its packaging and DO NOT place it in the BACTRON900 at this time!
Each O2 scrubber comes from the factory ready for use. If a scrubber has been stored at your site for longer than 6 months, it will need to be reactivated prior to installation for use. Reactivate the scrubber by baking at 200°C for 8 hours.
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INSTALLATION
PLACE ANAEROBIC MONITORING STRIPS
Place at least 5 anaerobic monitoring strip packets in the chamber. Do not place the packets in the incubator.
Do not open the packets at this time! Packets should only be opened in an anaerobic atmosphere. At least one of these strips will be used after establishing an anaerobic atmosphere. Up to a total of five may be required.

INSTALL INCUBATOR SHELF SPACERS
These spacers ensure even heat distribution and uniformity. 1. Set the spacers on the bottom shelf of the workspace chamber, side by side, with the "SPACER" label facing outward.
OPEN THE INCUBATOR DOORS
Open the incubator door by pulling the door handle open. The incubator doors must be open during the commissioning cycle while the BACTRON900 establishes an anaerobic atmosphere in its workspace chamber. Failure to do so will leave significant reservoirs of oxygenated atmosphere in the incubators.

BACTRON900

Spacer

Spacer Spacer

Incubator Door Handle

30 | P a g e

INSTALLATION

CLOSE THE AIRLOCK DOORS
The airlock doors should be closed and latched prior to launching a commissioning cycle. The inner airlock door will lock when the unit is turned On.
1

INSTALLING THE ARMPORT DOORS

The armport doors must be installed in order for the commissioning

cycle to successfully establish an anaerobic atmosphere.

1. Turn the locking bar on both doors to a roughly 45° position.

2. Insert the tabs for one door into the slots on the bottom of its armports.

3. Pull the top of the door toward you so that it sits balanced and vertical in the armport.

· Repeat steps 2 and 3 for the second door.
4. Turn the locking bars on both doors to a horizontal position, one at a time.

5. Secure the doors one at a time by turning the black armport doorknob clockwise.

o The silver locking bar will move toward the body of

the door.

o Use wrist strength only. Turn until the knob grabs and feels snug. Tightening too much can damage the door.

6. Gently check that the doors sit snug in the ports.

o The doors should not move when gently pulling on

the locking bar or pushing against the door body.

User finger strength only.

o The locking bar should not move.

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GRAPHIC SYMBOLS

The BACTRON900 has multiple graphic symbols located on its exterior and interior surfaces. The symbols identify hazards and the functions of the adjustable components, as well as important notes found in the user manual.

Symbol

Definition

Consult the user manual. Consulter le manuel d'utilisation
I/ON O/OFF: Controls all power to each chamber and its systems. I indique que l'interrupteur est en position marche. O indique que le commutateur est en position d'arrêt. AC Power Repère le courant alternatif
Low AMG supply alarm Indique l'alarme d'alimentation de gaz basse AMG
Airlock Anaerobic environment Indique un environnement anaérobie dans le sas
Temperature display Indique l'affichage de la température
Incubator heating Indique que l'incubateur chauffe
Adjusts UP and DOWN Ajuster la température de l'incubateur vers le haut et vers le bas
Over Temperature Limit Thermostat température limite contrôle haute

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GRAPHIC SYMBOLS

Symbol

Definition
Anaerobic Commissioning cycle in progress Signale un cycle de purge d'oxygène
Indicates AMG Indique gaz AMG
Foot pedal control / Sleeve Cycle Indique la commande de la pédale
Indicates potential shock hazard. Risque de choc électrique
Recycle the unit. Do not dispose of in a landfill. Recycle l'unité. Ne jetez pas dans une décharge
Protective earth-ground. Terre électrique
Manually adjustable. Indique un réglage manuel

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CONTROL PANEL OVERVIEW
Figure 5: Main Control Panel
MAIN PANEL INCUBATOR CONTROLS
BACTRON900
Figure 6: The Main Control Panel operates the workspace chamber incubator.
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CONTROL PANEL OVERVIEW
Main Panel Incubator Controls continued
Status
Power Switch Controls all power to each chamber and its systems.
Low AMG Supply Alarm If the BACTRON900 detects low pressure in the external AMG supply line lasting longer than 30 seconds, it will illuminate the Low AMG Supply indicator light and sound an alarm beep every 15 seconds. The BACTRON900 will abort any active cycles until pressure is restored in the line.
Airlock
Airlock Anaerobic This indicator light illuminates during the final gas backfill of an airlock auto cycle. It will remain lit until the next time the outer airlock door is opened, exposing the airlock chamber to aerobic air. Auto Cycle Pushing the rocker switch briefly initiates the airlock auto cycle. Pressing and holding the switch for at least 2 seconds aborts the cycle. The number of evacuations gas-backfill cycle iterations is set using the Airlock Cycle Setting Switch control located on the shadowbox control panel. Airlock Pressure Display This display shows the level of atmospheric pressure in the airlock in inches of mercury (inHg). At room pressure, the gauge should read 0. During cycles, the pressure in the airlock drops to -18inHg during evacuations and rises to -4inHg during the gas backfills. The final gas backfill is timed to end with a mild overpressure in the airlock (approximately 0.5inHg) This allows the inner airlock door to be opened without difficulty from inside the overpressure environment of the workspace chamber.
Incubator
Incubator Temperature Display During normal operations, the display shows the current incubator air temperature, accurate to 0.1°C. The Up and Down buttons are used to change display modes and input either a new temperature setpoint or a calibration adjustment. The display blinks continually while in its setpoint or calibration adjustment modes, preceded by an "SP" for Setpoint or "C O" for Calibration Offset. The heating indicator light illuminates when the BACTRON900 calls for power to the incubator heating elements.
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CONTROL PANEL OVERVIEW
Main Panel Incubator Controls continued
Over Temperature Limit The red Over Temp Activated light illuminates when the Over Temperature Limit system cuts off heating in the incubator by rerouting power away from the heating elements. While the OTL is rerouting power an alarm beep sounds a short alert every three seconds. The OTL control dial is located on the side shadowbox control panel. For more details, please see the Over Temperature Limit System description in the Theory of Operations (page 41).
Workspace
Commissioning Cycle in Progress This indicator illuminates while the automated Anaerobic Commissioning Cycle is active. The commissioning cycle launch switch is located on the shadowbox control panel.
AMG Injecting This illuminates while the BACTRON900 is injecting AMG into the workspace chamber.
Sleeves
Sleeve Cycle in Progress The Sleeve Cycle light illuminates while the BACTRON900 is cycling the sleeves and armports.
Sleeve Cycle The foot pedal switch launches the sleeve cycle. Momentarily pressing the foot switch initiates the cycle. Press the switch a second time to cancel the cycle.
Sleeve Pressure Display The display shows the pressure in the sleeve assemblies. At room pressure, the gauge should read 0. During sleeve cycles, the pressure drops to -18inHg, then briefly backfills with AMG. During normal operations when a cycle is not running, a pressure of +0.1inHg or higher on this gauge causes the BACTRON900 to open its chamber vent solenoid. This provides dynamic venting that helps prevent the manometer from bubbling (outgassing chamber atmosphere) while a user is working in the chamber, and displacing atmosphere within the sealed volumes with their arms.
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CONTROL PANEL OVERVIEW
SHADOWBOX CONTROL PANEL
Located on the left side of the BACTRON900 behind a sliding door. This panel contains setting and backup manual controls that are typically not used during routine operations.
Manual Switch The Manual Gas / OFF / VAC switch is a backup system that allows the user to manually cycle the airlock.
Airlock Cycle Setting Switch This switch sets the number of evacuations gas backfill iterations for the airlock auto and manual cycles. The BACTRON900 comes set to 3 cycle iterations but can run 4 or five iterations.
Controller Alarm Volume The top Audible Alarms dial sets the beep volume for the cycle abort alarms and the Low AMG warning alarm. The beep volume ranges from high to mute.
Over Temp Alarm Volume The bottom Audible Alarms dial sets the beep volume for the Over Temperature Limit alarm. The volume ranges from high to mute.
Over Temperature Limit Control On the BACTRON900 this dial sets the Workspace Chamber Incubator heating cut-off limit.
Commissioning Cycle Switch Pressing this switch for up to 5 seconds launches the auto-timed Anaerobic Commissioning Cycle, purging oxygenated atmosphere from the chamber. Pressing and holding the switch for 5 seconds or longer again aborts the cycle.
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CONTROL PANEL OVERVIEW BACTRON900 WORKSPACE INCUBATOR
The main panel incubator controls operate the side storage incubator.
Figure 7: BACTRON900 Workspace Incubator Control Panel
The workspace incubator control panel on the BACTRON900 controls the operations of the workspace chamber incubator and Over Temperature Limit (OTL) system.
Incubator Display During normal operations, the display shows the current incubator air temperature, accurate to 0.1°C. The Up and Down buttons are used to change display modes and then input either a new temperature setpoint or a calibration adjustment. The display blinks continually while in its setpoint or calibration adjustment modes, preceded by an "SP" for Setpoint or "C O" for Calibration Offset. The Heating indicator light illuminates when the BACTRON900 calls for power to the incubator heating elements.
Set Over Temperature This graduated dial sets the heating cut-off point for the OTL temperature limit system. The OTL system prevents unchecked heating of the chamber in the event of a hardware failure or external heat spike. For more details, please see the Over Temperature Limit System description in the Theory of Operations (page 37). The red Over Temp Activated light illuminates when the Over Temperature Limit system cuts off heating by rerouting power away from the heating elements. An alarm beep sounds every 3 seconds while the OLT is rerouting power. Over Temperature Alarm Volume The Over Temperature Alarm volume is located on the back of the control box and is for the workspace incubator.
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CONTROL PANEL OVERVIEW
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OPERATION
THEORY OF OPERATION
Achieving Anaerobic Conditions
Figure 8: Atmosphere Control Measures The BACTRON900 is engineered to establish and maintain an anaerobic workspace chamber atmosphere suitable for clinical cultivation of anaerobic bacteria. This atmosphere is initially achieved by purging the chamber with pulses of anaerobic mixed gas (AMG) as part of an auto-commissioning cycle. The AMG purge pushes standard (free) atmosphere out through a vent solenoid controlled by the cycle. In addition to the AMG pulses, an O2 scrubber inside the chamber captures oxygen through a catalytic reaction between the AMG hydrogen, any free oxygen, and the palladium-coated pellets in the scrubber cartridge. This reaction forms water vapor (H2O). Catalysis is the process of increasing the rate of a chemical reaction by adding a substance known as a catalyst. Catalysts are not consumed in the reaction and remain unchanged after it. In the BACTRON900, the catalysis is an exothermic process, and the scrubber cartridge body will grow hot to the touch in the presence of free oxygen and hydrogen. After completion of the auto-commissioning cycle, the O2 scrubber maintains the anaerobic atmosphere in the chamber. Each O2 scrubber must be removed after 24 hours of use and reactivated by baking the cartridge at 200°C for 8 hours. This removes buildups of hydrogen sulfides and fatty acids from the palladium surfaces of the scrubber pellets. A mild overpressure in the chamber is established with AMG injections to prevent the infiltration of external aerobic atmosphere, including the diffusion of molecular oxygen through seals. Oxygen intrusion surveillance is conducted using color-changing Oxoid brand indicator strips. Microbiological controls, such as Clostridium novyi or Pseudomonas aeruginosa, may also be used to indicate anaerobic or aerobic conditions. A digital oxygen detector is available for purchase for real-time readings and logging. Condensate Management Evaporation from Petri dish sample media and water vapor from the catalytic scrubber reaction are captured on the cold plate of a Peltier-effect condensate chiller located behind the O2 scrubber cartridge. This condensed moisture is then channeled into a drain tube that empties into a receptacle placed in the workspace chamber by the end-user. The receptacle must be drained regularly. The Peltier condensate chiller eliminates the need to use chemical desiccants which can retain condensate and dry out culture media.
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OPERATION
Accessing the Workspace Chamber The BACTRON900 airlock is used to introduce or remove sample containers and laboratory equipment from the workspace chamber. The airlock creates a near anaerobic environment through partial vacuum evacuations to reduce the atmospheric volume, followed by anaerobic gas backfills. Each evacuation phase draws the airlock down to -18 inches of mercury (inHg), removing approximately 60% of the O2 in the airlock while retaining enough pressure to prevent sample media from boiling. The number of evacuations backfill iterations can be set by the user to 3, 4, or 5. More iterations use more gas and require more time but achieve a lower end-state oxygen residual in the airlock chamber. Residual oxygen is captured in the workspace chamber by O2 scrubber catalyst, which is located in close proximity to the inner airlock door and the chamber circulation fan.
A backup control to manually cycle the airlock is located on the shadowbox control panel.
Users can access and work glove-free in the anaerobic workspace chamber using the armports and attached sleeves on the front of the BACTRON900. The user dons both sleeves and initiates a cycle of the sleeves by pressing the foot pedal once. Upon completion of the cycle, the user opens the armport doors and enters the chamber. The automated sleeve cycle consists of two vacuum down/AMG backfill iterations.
Cycling and effective use of the sleeves require bare skin contact between the widest part of the user's forearms and the cuff ring of the sleeve body. Smooth small items held by hand may be introduced into the workspace chamber through the sleeves. The sleeves are compatible with exam gloves for handling pathogenic samples.
Incubators The BACTRON900 has a side-storage incubator adjacent to the workspace chamber.
The incubator is controlled by a microprocessor board with a solid-state temperature sensor probe attached to the incubator body, along with two heating elements. The processor employs proportionalintegral-derivative (PID) analytical feedback-loop functions when measuring and controlling the chamber air temperature. PID-controlled heating pulse intensities and lengths are proportional to the difference between the measured chamber temperature and the setpoint. The setpoint is the desired operating temperature entered by the user. The frequency of pulses is derived from the rate of change in the difference. The integral function slows the rate of pulses when the temperature nears the setpoint in order to prevent overshooting.
The PID functions are also used to optimize incubator warming rates for hotter or cooler environments. If the BACTRON900 is moved to a new location with a significant temperature difference, it may require 24 hours of incubator runtime for the processor to fully adapt to the new thermal environment. To verify the temperature display accuracy, the incubator should run at its application setpoint for 24 hours before loading samples. The heat loss from leaving the incubator doors open for long periods (an hour or more) can trick the controller into operating as though in a cool environment. This can result in a phase of temperature overshoots.
The incubator relies on natural heat radiation for cooling. An incubator can achieve a low-end temperature of the ambient room temperature +5°C. The fan inside the incubator aids in maintaining air circulation and a uniform air temperature in the incubation space.
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OPERATION
The Over Temperature Limit (OTL) System The OTL is a mechanical heating cutoff included with each incubator and operates independently of the incubator microprocessor controller. The OTL helps safeguard samples by preventing runaway heating in the event of a hardware failure in the microprocessor controller or a sudden external heat spike. The OTL is connected to a hydrostatic sensor probe located inside the incubator and is intended to be set by the user to approximately 1°C above the current operating temperature setpoint. If the incubator temperature exceeds the OTL cutoff setting, the OTL will route power away from the incubator heating elements. It will continue to do so as long as the incubator air temperature is higher than the present OTL cutoff setting. While the OTL is rerouting power, a red indicator illuminates, and a short alarm beep sounds every 3 seconds. Manometer Pressure Gauge and Check Valve The water-filled manometer in the workspace chamber serves as a visual pressure gauge and dynamic venting check valve during instances of excess overpressures. The manometer is filled to the top reference ring when the chamber is unpowered and at room pressure. When the BACTRON900 is powered, the chamber is under pressure which forces the water down to 0.5 inch from the bottom reference ring.
Figure 9: Chamber Manometer
Additional pressure increases inside the workspace chamber to drive the water farther down into the manometer bottle. Excessive pressure will cause the water to bubble as the chamber atmosphere is vented through the manometer water and out of the chamber. This helps to prevent damage to the chamber gaskets and the acrylic glass panels. The manometer exhaust vent is a port consisting of a tube and black O-ring located on the back, right side of the BACTRON900. Additional venting is provided by the armport sleeve solenoid whenever the sleeve pressure gauge detects a pressure level of +0.1inHg or higher in the sleeve assemblies.
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OPERATION
PUT THE BACTRON900 INTO OPERATION
Note: Once in operation, the BACTRON900 should run for 24 hours prior to loading samples. This ensures the stability of both the anaerobic atmosphere and incubator air temperature.
After installation in a new workspace environment, confirm the following items are available prior to placing the unit into operation.
1. Verify that all Installation procedures have been carried out. 2. Verify enough AMG is on hand to commission and sustain an anaerobic atmosphere. 3. Verify that an active O2 scrubber is ready to install in the workspace chamber.
o The catalytic O2 scrubbers come from the factory activated and ready for use. o If the scrubber cartridge has been stored for 6 months or longer, bake out the
scrubber cartridge for at least 8 hours at 200°C to reactivate the catalytic palladium. Perform the following procedures and steps
1
Perform the Install the O2 Scrubber procedure on page 47.

2
3
A M G
4

Perform the Plug in the BACTRON900 procedure on page 47. Perform the Supply Gas to the BACTRON900 procedure on page 48.
Place the Power Switch on the Main Control Panel in the On (|) position (page 44).
· The switch and control panel displays will illuminate.
44 | P a g e

OPERATION
5

Zero the Pressure Displays if the BACTRON900 has been installed in an overpressure environment or at a high altitude. This sets the pressure gauges to local conditions.

Leave the incubator Set to Off
Each BACTRON900 incubator comes from the factory set to OFF and must remain off during the auto commissioning cycle. The incubator display shows a temperature near that of the ambient environment when off.
If the incubator has been turned ON, see the Set the Incubator Temperature on page 58 for how to set an incubator to OFF.
6
Commissioning Cyle Perform the Launch the Anaerobic Commissioning Cycle procedure on page 49. The cycle takes approximately 5 hours to complete for the BACTRON900.
7
Attach the Sleeves to the armports, page 51.

8
Read

Review the following procedures while the commissioning cycle establishes an anaerobic atmosphere. The user's hands will be occupied in the sleeves while working in the chamber after the cycle is complete.
· Enter the Chamber page 52 · Moving in the Chamber page 54 · Anaerobic Monitoring Strip page 55 · Verifying the Anaerobic Atmosphere page 56 · Troubleshooting O2 in the Chamber page 69 · Exit The Chamber page 57

Commission in Progress

Commissioning Cycle Finishes
· The Commissioning cycle light will flash three times, then extinguish.

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OPERATION
10

Enter the Chamber after the commissioning cycle has finished, page 52.

Verify the Anaerobic Atmosphere, page 56.

If the chamber is not fully anaerobic perform the Troubleshooting O2 in the Chamber, page 69.

Optional: If you are required to verify the accuracy of the

incubator temperature display(s), set up the verification

equipment now.

See the suggested calibration setup and the first two steps of the suggested calibration procedure on pages 80 and 81.

Close the 3 incubator doors.

· If you have set up temperature probes in the incubator(s) to run a verification or calibration, make sure any gaps created by the probe wires are covered.

Set the incubator to your application temperature. Please see

the Set the Incubator Temperature procedure on page 58.

Allow the BACTRON900 to run 24 hours prior to:

24 Hours Required

· Loading Samples, page 63
· Verifying or calibrating the accuracy of the incubator temperature display.

Set the Over Temperature Limit heating cutoff temperature on

page 59.

BACTRON900: The OTL system for the incubator must be set separately.

Step 16 concludes the Putting the BACTRON900 into Operation procedure.
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OPERATION
INSTALL AN O2 SCRUBBER CARTRIDGE
Open the left armport door, install one O2 scrubber cartridge, then close and latch the armport door.

See page 31 for instructions on Properly Closing and Latching the Armport Door. Whenever the chamber is anaerobic, scrubbers should be introduced and removed through the airlock.
Note: Incubator not shown in renderings above.
PLUG IN THE BACTRON900

All Units

47 | P a g e

OPERATION
SUPPLY GAS TO THE BACTRON900
1. Open the supply cylinder valve.

Example only. Supply cylinder valve open close directions vary by country.

The supply gauge (right) will jump from zero ( 0 ) to showing the current gas supply pressure level.

2. Open the Regulator Flow Valve to supply an AMG flow of 50 psi to the BACTRON900.

AMG 50 psi

2 - Or -
3

AMG 50 psi

A M G
N2

N2 50 psi

3. Optional: Open the Nitrogen regulator valve to supply a N2 flow of 50 psi to the BACTRON900 N2 port.
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OPERATION
ZERO THE PRESSURE DISPLAYS
Conditional: This procedure should always be performed on BACTRON900s installed in overpressure environments, and may also need to be performed on units installed in high-elevation locations 5,000 feet (2,000 meters) above sea level or higher. Function: The Airlock and Sleeve Pressure Displays on the main control panel should each show 0 (zero) when exposed to room atmosphere pressure. The gauges help restore the airlock and sleeves to near room pressure when completing a cycle. The gauges were originally zeroed near sea level.
Sleeve Pressure Display Zero the Sleeve display if the display shows a reading other than 0 when sleeves are not attached to the armports.
1. Always remove the sleeves from the armports prior to zeroing. 2. Press and hold both the up and down buttons until the display shows zero.
Airlock Pressure Display Zero the Airlock display if the display shows a reading other than 0 when the outer airlock door is open, exposing the airlock chamber to room pressure atmosphere.
1. Open the outer airlock door. 2. Press and hold both the up and down buttons until the display shows zero. 3. Close and latch the door after zeroing the display.
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OPERATION
LAUNCH THE ANAEROBIC COMMISSIONING CYCLE
The anaerobic commissioning cycle establishes an anaerobic atmosphere in the workspace chamber over the course of several hours.
Note: Commissioning cycles will not initiate while a sleeve cycle or an airlock cycle is active. 1. Prior to launching the cycle, verify that: Incubator is set to OFF

All 3 Incubator doors are open

Sufficient AMG is available.

Both airlock doors are closed and latched

Armport doors are closed and latched.

2. Launch the Commissioning Cycle:
Briefly press the Commission Auto Cycle button on the side shadowbox control panel.
· The Commission in Progress light on the main control panel will illuminate and remain on throughout the cycle.
o The BACTRON900 requires 5 hours to complete the cycle.
During the cycle, extended AMG injections force aerobic chamber atmosphere out through a one-way commissioning solenoid. This purge and the O2 capture provided by the oxygen scrubber removes O2 from the chamber atmosphere.
· Sleeve cycles and airlock cycles will not initiate while commissioning is active.
· Upon completion of the Commission Auto Cycle, the Commission in Progress light will blink three times and extinguish. The commissioning solenoid port closes automatically.
Fogging and Humidity. Mild or heavy condensation may take place inside the chamber during the commissioning cycle. This is due in part to the formation of water vapor as the catalytic O2 scrubber removes large amounts of oxygen. High ambient humidity and cool room temperatures also contribute. The condensate should dissipate by the end of the cycle as oxygen decreases and as the condensate controller removes water vapor from the chamber atmosphere.
Aborting the Commissioning Cycle
If it is necessary to abort the commissioning cycle, press and hold the Auto Commissioning Cycle switch for 5 seconds.

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OPERATION

ATTACH THE SLEEVES
Attach and secure both sleeves to the armports. This allows reach-in access to the chamber through the ports without introducing aerobic external atmosphere.

Figure 9: Sleeve Installation
Begin with either Armport 1. Unroll the large opening of a sleeve over the lip of the armport door. Beginning from the
bottom of the armport is typically the easiest approach. · Place the ring on the large end of the sleeve inside the groove on the armport. · Make sure none of the sleeve material is trapped or pinched between the ring and the
seating groove. 2. Secure the sleeve to the armport using the 48 inch (121 cm) self-gripping strap included with
the sleeve. · Exercise caution when placing the strap next to the armport gas lines.
3. Repeat the process for the second sleeve and armport. Note: Sleeves may remain attached to the BACTRON900 when not in use.
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OPERATION ENTER THE CHAMBER
Prior to entry, read the Exiting the Chamber procedure (page 57) for how to withdraw your arms from the chamber without compromising the anaerobic atmosphere. 1. Don the Sleeves
Note: Sleeves come with mid-sized, size 8 cuffs. Please see the Parts List on page 88 for other cuff sizes.

Remove rings and watches. Sharp objects will damage the sleeves.
Snug contact, sleeve cuff and bare skin at the widest part of the forearm.
2. Position and hold your hands approximately 4 6 inches (10 cm 15 cm) away from the armport doors, to either side.
This position prevents the collapsing sleeves from pulling your hands into the arm port doors during the vacuum down cycle phases. It also keeps the sleeve material tight, helping to obtain a complete evacuation of the sleeves.

3. Cycle the Sleeves
a. Press and release the foot pedal. · Both sleeves will vacuum down then partly fill with AMG twice.
Cancelling the Sleeve Cycle: Press the foot pedal at any time to end an active sleeve cycle.
Auto Abort Signal: The Auto Abort Signal is an audio alarm of a long beep, followed by a short beep sound. The sleeve cycle light flashes in the event of an auto abort.

Auto Abort 1: The cycle will abort if a vacuum down phase fails to achieve -18inHg within 25 seconds. Check the sleeves for leaks and ensure that the sleeves are properly attached.

Auto Abort 2: The cycle aborts if the cycle injects AMG into the workspace chamber twice during a sleeve cycle vacuum phase. Check that the arm port doors are properly sealed and latched to prevent the sleeve cycle from vacuuming atmosphere out of the chamber.

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OPERATION
4. Open the Armport Doors
a.
b.
c.
1 2
5. Store the Armport Doors

When the cycle has finished, loosen both armport doorknobs by 2 or 3 turns.
Rotate the locking bars to roughly 45°.
Slowly push one door into the chamber, then slowly push the other door into the chamber. Pushing both doors in simultaneously will create a significant displacement of the pressurized chamber atmosphere, resulting in active venting.

Open and center the 2 Incubator Doors in the Chamber, and open the 1 door that accesses the Side-Storage Incubator for the Commissioning Cycle

Armport Doors and Armport Door Stand

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OPERATION
MOVING IN THE PRESSURIZED CHAMBER
Undisturbed Overpressure
When sealed and sitting undisturbed, the BACTRON900 maintains 0.5 inches (1cm) of water column overpressure in the workspace chamber to prevent infiltration by external atmosphere.

Incubator not shown here or below.

Pressure Increase

0.5 inches (1cm) water column overpressure

Introducing objects into the sealed chamber displaces atmosphere, further increasing the pressure. The BACTRON900 will vent atmosphere through a pair of one-way valves to avoid damage to the chamber.

Sleeve vacuum solenoid vents for up to 60 seconds when the sleeve pressure exceeds +0.1inHg.

Manometer Venting water bubbles

Reminder: Approximate volume of displaced chamber atmosphere is equivalent to inserted arms.

Water in the manometer's outer bottle forced down.

All vented atmospheres will be replaced with injected AMG after the displacement ends. This drives up the overall AMG usage and associated operating costs.
Pressure Management
· Slow, deliberate, simultaneous movements balancing out one another.
· No atmosphere vented.

Use a swimming motion, withdrawing one arm while reaching in with the other. Slow movements avoid spiking the chamber pressure and venting anaerobic atmosphere.
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OPERATION
VACUUMING DOWN BALLOONING SLEEVES
The foot pedal switch may be used to vacuum down the sleeves if the sleeves balloon up when 1 working in the workspace chamber.
2 1. Tap the foot pedal switch once to begin vacuuming down. 2. Tap the foot pedal again when the sleeves have collapsed far enough to work comfortably. Note: If the foot pedal is not pressed a second time, the BACTRON900 will run through a complete
sleeve cycle.
ANAEROBIC MONITORING STRIPS
An anaerobic monitoring strip packet must be opened to verify a strict anaerobic atmosphere has been established in the chamber following the commissioning cycle. An open strip must be present at all times in the chamber during normal operations to monitor for oxygen contamination. Handling and Placing the Strips
· Do not touch the monitoring strip body. Doing so risks contaminating the strip and creating false oxygen positive coloring, even in a fully anaerobic environment.
· A dried-out monitoring strip will not indicate new oxygen contamination. A new strip must be opened in the chamber every 24 hours.
· Place the strip where it will be clearly visible. o Do not place the strip in an incubator. Accelerated drying and heat discoloration may generate false positive readings.
· For best accuracy and to lengthen the amount of usable time, the strip should be left in the packet, partly exposed. This avoids contamination and wicks oxygen-detecting fluid up from the bottom of the packet. o Bend the edges or partly fold the packet to allow it to stand upright.
· The strips do not lose color after having been exposed to significant O2. A strip holds its pink coloration once completely saturated, even if the oxygen is removed from the chamber atmosphere. o A new packet must be opened to resume monitoring for O2 once all oxygen is purged or scrubbed from the chamber atmosphere.
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OPERATION

Note: A digital gas analyzer capable of detecting oxygen may be used to verify an anaerobic atmosphere has been established. Use the airlock to introduce the analyzer into the workspace chamber after the commissioning cycle is complete.

VERIFY THE ANAEROBIC ATMOSPHERE
1. Enter the workspace chamber using the Entry procedure, cycling the sleeves. 2. Open 1 oxygen strip near the center of the chamber. 3. Wait 1 minute. Observe the strip handling precautions listed on page 54.

White

Moderate Pink

Anaerobic

Heavy O2

Very Pale Pink

Saturated Pink

Slight O2

Fully Aerobic

Light Pink

Mild O2

4. If the strip remains white, the chamber is anaerobic. Exit the chamber using the steps in the Exit the Chamber procedure on page 56. The verification procedure is complete.
· Leave the strip in the chamber to monitor for oxygen intrusions.
Or
5. If the strip turns partly or completely pink, exit the chamber using the Exit the Chamber procedure on page 57.
6. Launch another BACTRON900 commissioning cycle to purge oxygen and introduce more AMG for the scrubber to remove O2.
· Allow the cycle to run for 1 hour.
7. Abort the commissioning cycle after 1 hour.
8. Reenter the chamber using the Entry procedure and open 1 anaerobic monitoring strip.
9. If the strip remains white, the chamber atmosphere is anaerobic and ready to use. Exit the chamber using the steps in the Exit the Chamber procedure.
· Leave the strip in the chamber to monitor for oxygen intrusions.
Or
10. If the second strip turns pink, exit the chamber, and perform the Troubleshooting Oxygen in the Chamber procedure on page 69.

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OPERATION
EXIT THE CHAMBER
Pressure in the chamber drops when a user withdraws their arms. If done too quickly, this can draw in outside air through the sleeve cuffs or manometer. Use the following steps to exit the chamber without pulling in aerobic atmosphere.
1. Check that both airlock doors are closed and latched to avoid drawing aerobic atmosphere in through the airlock.
2. One at a time, remove armport doors from storage and place them on the chamber floor in front of the ports.
3. Close and latch the armport doors. · See the Install the Armport Doors procedure on page 30 for how to correctly latch the armport doors.
4. Withdraw your arms from the sleeves one at a time.
Armport Seal Check
1. Grip and slowly push both sleeves simultaneously towards the doors. This will cause the sleeves to balloon. · If the manometer water bubbles in response or the outer ring of water moves down, one or both armport doors are not sealed. · The armport vacuum solenoid will click and passively vent atmosphere from the sleeve assemblies responding to the pressure increases in the sleeves, even if the doors are sealed correctly. · Do not physically press on the armport doors to test the seals! Doing so routinely may warp the acrylic glass front panel or damage the doors and armports.
2. If the armport doors are not correctly sealed, don and cycle the sleeves, then reseat and relatch the doors.
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OPERATION
SET THE INCUBATOR TEMPERATURE SETPOINT
Close the incubator doors prior to setting a temperature setpoint. Running the incubator for longer than a half hour will result in temperature instability and overshoots once the doors are closed.
BACTRON900: Each incubator must be set independently.

1. Set OTL control to its maximum setting, if not already set to max.
· Turning the OTL all the way to the right (clockwise) prevents the heating cutoff system from interfering with this procedure.

2. Jump to the Temperature Setpoint Adjustment mode

Set Temperature

Set Tempe ature

Setpoint Adjustment Mode

Initial Setpoint+

Press and hold either

Note: The display will automatically exit the adjustment mode after 5 seconds of inactivity on the arrow keys, saving the last shown setpoint value.

3. Set the Temperature Setpoint

Note: To turn an incubator off, set the setpoint to its lowest setting (OFF).

Adjust

New Setpoint

4. Wait for 5 seconds after entering the Setpoint

Set Temperature

HEATING ACTIVATED

· The display will stop flashing. The setpoint is now saved in the controller.
· The display will revert to showing the current chamber air temperature, heating or passively cooling to match the new setpoint.

End of procedure

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OPERATION
Note: Test the OTL heating cutoff system at least once each year for functionality.
SET THE OVER TEMPERATURE LIMIT
The incubator must be operating at your incubation application temperature and must be stable for at least 1 hour prior to setting the OTL. The incubator OTL must be set independently in the BACTRON900.
1. Set OTL control to its maximum setting, if not already set to max.
2. Turn the dial counterclockwise until the red Over Temperature Limit Light illuminates.

3. Slowly turn the dial clockwise until just after the OTL Activated light turns off.
· The Over Temperature Limit is now set approximately 1°C above the current incubator air temperature.
4. Leave the OTL dial set just above the activation point.

Optional: Turn the dial slightly to the left.
· This sets the OTL cutoff threshold nearer to the current incubator air temperature.
If the OTL is sporadically activating, you may turn the dial very slightly to the right (clockwise).
If the OTL continues activating, check for ambient sources of heat or cold that may be adversely impacting the unit's temperature stability. Check to determine if any powered accessories in the workspace chamber are generating heat. If you can find no sources creating external or internal temperature fluctuations, contact Tech Support or your distributor for assistance.

End of Procedure

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OPERATION

SET THE AIRLOCK CYCLE ITERATIONS
Optional: The BACTRON900 comes from the factory set to run a 3-iteration auto cycle. The airlock can be set to run 4- or 5-iteration cycles. Each cycle iteration consists of a vacuum down evacuation phase followed by a gas backfill of the airlock chamber. More iterations decrease the amount of O2 left in the airlock chamber upon cycle completion but increase gas usage and cycle run times.

· BACTRON900:
o 3 cycle iterations, 46 seconds o 4 iterations, 60 seconds o 5 Iterations, 72 seconds.

Figure 10: Shadow Box Control Panel

CYCLING THE AIRLOCK

Light On. The airlock has been cycled. Inner airlock door unlocked.

Outer Door Inner Door

Light Off. The outer airlock door has been opened allowing O2 into the airlock. Inner door is locked.

Cycle the airlock prior to opening the inner airlock door whenever the Airlock Anaerobic light is off.

Auto Cycle

1. Close and latch both the inner and outer airlock doors, or the airlock will not cycle.
2. Push the Airlock Auto Cycle switch on the main control panel.
· The airlock evacuates down to -18inHg then backfills with gas drawn from the N2 In port to -4inHg during all but the final cycle iteration.
· The airlock backfills to a positive pressure drawing from the AMG In Port during the final gas backfill. The Airlock Anaerobic light turns On.
· The inner airlock door unlocks during the final gas backfill.
The inner airlock door locks automatically when the outer airlock door is opened.

Always close airlock doors after loading or offloading items. This safeguards against an inattentive user opening the outer door while the inner door is open.

Aborting an Active Auto Cycle

1. Press and hold the Auto Cycle switch to the right for 2 seconds.

· The BACTRON900 will cease vacuuming and automatically restore pressure to the airlock.

Simultaneous Cycles

Cycling the armports while cycling the airlock slows the airlock cycle.

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OPERATION

MANUALLY CYCLING THE AIRLOCK
This control is intended as a backup for the airlock autocycle system. It can also be used to carry out custom cycles or low-pressure applications down to -18inHg in the airlock chamber.
Unlocking: The inner airlock door will unlock when the chamber has been vacuumed down a number of times equal to:
· The Auto Cycle selector switch setting when the last autocycle was run; or,
· The door unlocks after the last autocycle when the BACTRON900 is turned off. The airlock vacuums down a number of times equal to the switch setting when the unit is turned back On.
Both airlock doors must be closed and latched. The airlock will not manually cycle if either door is open.
1. Press the Manual Cycle switch to the left (VAC).
· The airlock will draw down to -18inHg pressure and then cease vacuuming.
· You may stop the vacuum down at any time by returning the switch to the middle position.
o Stop the vacuum draw down immediately if the water in the manometer gauge bubbles or the AMG Injecting light illuminates frequently indicating there may be a leak along the inner airlock door.
2. Press the Manual switch to the right (GAS) to inject AMG into the airlock.
· The airlock will backfill with AMG, restoring it to near room atmosphere pressure.
· You may stop the AMG backfill at any time by returning the switch to the middle position.
· This completes one cycle iteration.
3. Run a number of cycle iterations, repeating Steps 1 and 2, equal to the number of iterations run during the last autocycle.
· The inner airlock door unlocks when the Manual Cycle switch is returned to the middle position after completing the final vacuum down for the cycle.

MANUAL CYCLE

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OPERATION AIRLOCK SAFETY ABORTS
An auto cycle will abort under the following conditions. · If either airlock door is opened during the cycle. · If the Low AMG alarm activates. · If the vacuum pump has been running continuously for 90 seconds. · If an auto cycle fails to achieve a pressure lower than -4inHg in the airlock chamber within 10 seconds during a vacuum down phase. o This safeguards against any leaks in the airlock door seals drawing in external aerobic air or workspace chamber atmosphere.
In the event of an aborted auto cycle, a long beep followed by a short beep will sound, the Airlock Anaerobic light will flash, then extinguish.
VACUUM PUMP COOLDOWN LOCKOUT
The BACTRON900 will stop running the vacuum pump after 90 consecutive seconds of operation. This safeguards against overheating the pump in the event of a leak in the workspace chamber, airlock, gas plumbing, or other hardware failures. This cooldown lockout lasts for five minutes during which the airlock and sleeve cycles will not run. Normal vacuum applications in BACTRON900 cycles require runtimes significantly shorter than 90 seconds. If the vacuum pump is in a lockout state, check for leaks in the workspace chamber and airlock.
INNER AIRLOCK DOOR LOCK
Locking The inner airlock door automatically locks when the outer airlock door is opened or when the BACTRON900 is turned On. Manual Unlock To unlock the inner airlock door without manually cycling or auto cycling the airlock:
· Rapidly cycle the Manual Cycle switch from GAS to VAC a number of times equal to the setting of the Auto Cycle switch when an auto cycle was last run. o Or, if the BACTRON900 has been turned Off, equal to the setting of the Iteration switch when the unit was turned On. o Or, the lock can also be physically unlocked by pressing the small silver stud on the lock, just above the inner airlock door latch. The back of a pen or other narrow, blunt object is recommended.
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OPERATION
LOADING SAMPLES
The manufacturer recommends waiting 24 hours after establishing an anaerobic atmosphere before loading samples into the unit. Containers Airtight containers can introduce significant amounts of oxygen into the anaerobic environment of the BACTRON900.
· Whenever possible, closed containers placed in the airlock should be loose-capped or ventilated to allow the airlock cycles to draw oxygen from the containers.
· Caps on empty syringes should be loosened if permitted by your laboratory or production protocol.
Sliding Shelf Transport The airlock sliding shelf can hold and transport up to 216 plates. Incubator Sample Placement
· Even spacing. Place samples and other media containers as evenly spaced as possible on the incubator shelves to allow for atmosphere circulation and better temperature uniformity.
· Humidifying. Placing an open beaker of water on the left side of the circulation fan on the bottom shelf of the workspace incubator in the BACTRON900 will help prevent samples from drying prematurely.
· If anaerobes sensitive to heat are being cultivated, it may be necessary to place an empty Petri plate at the bottom of each stack of the workspace incubator.
This concludes Putting the BACTRON900 into Operation portion of the Operation Section.
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OPERATION
HUMIDIFYING THE INCUBATORS
Placing a small number of Petri dishes or other open media containers in the BACTRON900 for several weeks may lead to excessive drying of sample media. A small open container such as a flask of 500ml of distilled water set on each shelf of the incubator can help to slow sample drying.
CHAMBER ACCESSORY POWER OUTLETS
BACTRON900s are provided with two accessory outlets located inside the workspace chamber on the left wall. The power switch on the main control panel controls power to these outlets.
· The outlets are intended to power low-draw equipment such as magnetic stirrers or a volatile compounds scrubber fan.
· Do not attach equipment drawing more than 1 amp from both outlets.
Waste Heat
Accessory equipment may heat the workspace chamber. This can affect the temperature performance of the incubator and may increase pressure in the sealed chamber through thermal expansion of the chamber atmosphere. Monitor the chamber pressure using the manometer and the incubator performance when using powered accessories inside the workspace chamber.
VOLATILE COMPOUND SCRUBBER AND REJUVENATION CYCLE
Activated carbon scrubber media may be placed in the workspace chamber to absorb volatile fatty acids and volatile sulfur compounds produced by sample cultivation. Scrubbing out volatiles reduces odors, reduces the filmy buildups on chamber surfaces, and prolongs O2 scrubber endurance during cultivation processes producing significant amounts of VFAs (volatile fatty acids) or VSCs (volatile sulfur compounds).
Please see the Accessories section on page 91 for information on scrubber media recommended by the manufacturer.
Use and Rejuvenation:
1. Place a 250-gram sample (one packet) of media in a 500ml beaker. Place another 250-gram sample into a second 500ml beaker. · A scrubber fan is available for purchase to place scrubber media in. The fan container increases the volatiles scrubbing rate. Please see page 91.
2. Place the first media sample in the workspace chamber. 3. Swap out carbon media samples after every 24 hours in the workspace chamber. 4. Carbon media must be reactivated by baking. For best results, reactivate carbon media the same
day it will be placed into the chamber. · Bake at 160°C for at least 2 hours. Use carbon scrubber media for 6 months, then discard.
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OPERATION
CONDENSATION AND THE DEW POINT
Relative humidity inside the BACTRON900 should never exceed 80% at 25°C. Exceeding this threshold can result in condensate forming on the incubator and workspace surfaces. Condensate will appear whenever the humidity level in the chamber reaches the dew point. The dew point is the level of humidity at which the air cannot hold more water vapor. The warmer the air, the more water vapor it can hold. As the level of humidity rises in a chamber, condensate will first appear on surfaces cooler than the air temperature. Near the dew point, condensate forms on any item or exposed surface that is even slightly cooler than the air. When the dew point is reached, condensate forms on nearly all exposed surfaces. Mild condensate may be present in BACTRON900 units fully loaded or loaded to near capacity with breathable media plates, depending on ambient temperature and humidity. Cold air blowing on the exterior of the BACTRON900 may help cause condensation in the workspace chamber by chilling the acrylic glass panels or metal bulkheads. Managing excessive condensation at humidity levels that overwhelm the BACTRON900 condensate controller depends on either lowering the humidity level in the chamber or increasing its air temperature.
Note: Rising or falling air pressure from the weather will adjust the dew point up and down in small increments. If the relative humidity in the BACTRON900 is already near the dew point, barometric fluctuations may push it across the dew point threshold.
If excessive condensate is forming in the BACTRON900 chamber, check the following: · Is the BACTRON900 exposed to an external flow of cold air such as an air-conditioning vent or a door to a cooler hallway or adjacent room? Block or divert the air, or move the BACTRON900. · Does the ambient humidity in the room exceed the stated BACTRON900 operating range of 80% relative humidity? If so, lower the room humidity. · Do the number of media containers in the BACTRON900 exceed its rating? Reduce the number of sample containers. · Remove or cap open containers of water or media. Drain the condensate controller catch vessel frequently. Do not drain the manometer.
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OPERATION
DEIONIZED AND DISTILLED WATER
Do not use deionized water for cleaning or humidifying the BACTRON900! While commonly available in laboratory environments, deionized water is an aggressive solvent that attacks most metal surfaces. The use of deionized water in a BACTRON900 voids the manufacturing defect warranty and may damage the unit. The manufacturer recommends the use of distilled water in the resistance range of 50K Ohm/cm to 1M Ohm/cm, or a conductivity range of 20.0 uS/cm to 1.0 uS/cm for cleaning and humidifying applications.

PRESSURE UNIT CONVERSION CHART

InHg

1 inHg

1 kPa

0.2953

1 Kgf/cm2 28.9590

1 bar

29.5300

1 psi

2.0360

1 mmHG 0.0394

1 mmH2O 0.0028

1 inH2O 0.0734

kPa
3.3863 1
98.0665 100
6.8947 1.3332 0.0098 0.2490

Kgf/cm2
0.0345 0.0102
1 1.0197 0.0703 0.0014 0.0001 0.0025

bar
0.3386 0.01
0.9806 1
0.0689 0.0013 0.0001 0.0024

psi
0.4911 0.1450 14.2233 14.5037
1 0.0193 0.0014 0.0361

mmHG
25.400 7.5006 735.55 750.06 51.7150
1 0.0029 1.86832

mmH2O
345.32 101.97 10000.27 10197.44 703.09 13.5954
1 25.399

inH2O
13.6087 4.01463 393.700 401.463 27.7707 0.53524 0.03970
1

Figure 11: Pressure Measurement Unit Conversion

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USER MAINTENANCE

Chamber Quality Control Check Sheet

Month:

Record Incubator Scrubber

Temperature

Changed

Date:

Condensate Receptacle Drained

AMG Cylinder Pressure Reading

Monitoring Strip Changed

You may copy this sheet for institutional use.

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USER MAINTENANCE
DAILY MAINTENANCE
1. Swap out the O2 scrubber cartridge in the chamber with a reactivated scrubber. 2. Bake out the O2 scrubber removed from the workspace chamber. 3. Empty the condensate collection container, as needed. 4. Change the anaerobic monitoring strip at least once every 24 hours or more often, as
needed. The strip in the workspace chamber must remain moist in order to indicate the presence of oxygen. 5. Check that the airlock gaskets are properly seated. 6. Check the sleeve cuffs for holes, tears, and other signs of wear that may compromise integrity. Replace, if necessary. 7. Verify the incubator is set to the correct temperature. 8. Record the gas regulator supply reading. · Tracking gas usage prevents supply shortages and provides a record for tracking the
emergence of any leaks in the gas system. 9. Optional: Swap out and reactivate volatile compounds scrubber media, if installed. Please
see the Volatile Compound Scrubber and Rejuvenation Cycle on page 64 in the Operations section. 10. Check the water level in the manometer. While the chamber is operating under its normal overpressure of 0.5 inch, the water level should be even with the lower of the two reference rings (red). 11. Clean and disinfect the workspace chamber in accordance with your laboratory or production protocols, or regulatory requirements.
NORMAL GAS CONSUMPTION
A sealed and undisturbed BACTRON900 will typically go for more than 30 minutes between gas injections into the workspace chamber. The AMG Injecting light will illuminate while pulsing gas into the workspace chamber, accompanied by a pair of audible clicks from the gas solenoid opening and closing. Airlock cycles, entering or exiting the armports, and working in the workspace chamber will temporarily increase the frequency of gas injections. When the BACTRON900 is sitting sealed and undisturbed, AMG injections every twenty to thirty minutes may indicate a small leak. Injections every 10 30 seconds in an undisturbed BACTRON900 indicate a major leak.
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USER MAINTENANCE TROUBLESHOOTING PERSISTENT OXYGEN IN THE CHAMBER
1. Ensure the armport doors are being correctly installed (page 31) and sleeve assemblies are correctly attached (see page 51).
2. Verify proper sleeve donning and armport entry and exit procedures have been used (see page 52.
3. Check if samples and closed containers have been improperly introduced into the chamber (see page 63). · Samples and containers should not be introduced until the BACTRON900 has run with an anaerobic atmosphere in the chamber for at least 24 hours.
4. Verify the manometer is filled with 2 fl oz (60 ml) water. 5. If the manometer water bottle was removed for filling, verify the bottle has been properly
screwed back into the manometer body. · An improperly threaded bottle allows chamber atmosphere to leak directly through the
manometer body, bypassing the pressure containment of the manometer water airlock. 6. Verify the AMG cylinder or in-house supply regulator is set to at least 50 psi to ensure
adequate pressure is delivered to the BACTRON900. · Failure to properly attach supply lines to both ports will allow O2 into the chamber
when the airlock is cycled. Check for kinks or damage to the AMG supply line.

7. If used, verify the N2 cylinder or in-house supply regulator is set to at least 50 psi to ensure adequate pressure is delivered to the BACTRON900.
8. Verify that the anaerobic monitoring strips are properly handled and not placed in an incubation chamber. The strips are heat sensitive and will register a false O2 reading from heat discoloration. See page 55. · Make sure the monitoring strip packets have not been opened in an oxygenated atmosphere prior to being placed in the chamber.
9. Verify the O2 scrubber cartridge is activated. · See the Testing the O2 Scrubber Cartridges procedure on page 74.
10. Set the airlock cycle to 5 iterations to reduce post-cycle oxygen in the airlock chamber. · The airlock should not be used until the BACTRON900 has run with an anaerobic atmosphere in the workspace chamber for at least 24 hours, except as a diagnostic.
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USER MAINTENANCE

LEAK DIAGNOSTIC UNIT IN USE PROCEDURE
Perform this procedure to check for leaks in and around the workspace chamber when the BACTRON900 is loaded with samples and cannot be taken out of use. Leaks can result from damage, long-term wear on BACTRON900 components, or user error.

Establish a Baseline

A baseline of AMG usage should be established before attempting to determine if the chamber is leaking. AMG usage increases when users access and work in the workspace chamber, therefore, the baseline should be established when the unit is sitting undisturbed.

1. Record the gas cylinder supply level at the end of the workday. Note the gauge level the next morning. Read the Normal Gas Consumption description on page 68.
2. If the BACTRON900 is using a significant amount of AMG overnight while sitting undisturbed, a gas leak is likely. Review the AMG cylinder readings recorded in the maintenance log to see if a period of increased usage or loss can be identified.
3. If the BACTRON900 is injecting AMG more frequently than every 30 minutes after sitting undisturbed overnight, there is likely a leak.
· The normal injection rate of every 30 minutes or more sustains the chamber overpressure.

Verify Chamber Overpressure

Verify that the manometer has been periodically refilled or topped off as part of daily maintenance. If filled correctly, the water in the manometer should sit even with the bottom measuring ring of the manometer while the BACTRON900 is on and automatically maintaining overpressure in the chamber.

1. If the manometer is: (a) correctly filled, and (b) the water level is not depressed to the lower reference ring, and (c) the BACTRON900 is not injecting AMG frequently, the unit may be failing to inject gas as required to maintain the chamber overpressure.
2. If the manometer water level is not depressed and the BACTRON900 is injecting AMG frequently, it is likely there is a significant leak.
Check the Airlock

0.5 inches (1.27 cm) water column pressure

Verify the integrity of the airlock if the previous steps indicate a leak.

1. Check the airlock door gaskets. There should be no brittleness or dryness, and no cracks.
2. Check that both gaskets are securely seated on the mounting frames. Sample media is sticky, and if spilled, can cause an airlock door to pull a gasket off the mounting frame.
· The airlock door windows should sit flush against the door gaskets when the doors are closed.

3. Confirm that users are closing the inner airlock door after transferring items into or out of the workspace chamber.

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USER MAINTENANCE
Check the Armport Doors Failure to correctly close and latch the armport doors can result in the chamber leaking anaerobic atmosphere and increasing the rate of gas injections while sitting undisturbed.
1. Check the door ring-seals for signs of damage or excessive wear. Replace the rings if there are obvious signs of damage or wear.
2. Check that the armport doors are sealed and secure when not in use. a. The locking bars should be in the horizontal position. b. The knobs should be tightened clockwise using only wrist strength. Tightening the knobs too tight can damage the doors. This can result in a leak of chamber atmosphere around the threaded post on which the knob is mounted. c. The door should sit snugly in the port when correctly sealed. Use finger strength only to check that the door does not rock in the port.
Locating Leaks A gas leak detector capable of detecting hydrogen (Part Number 4600501) can be used to locate leaks along the sealed edges of the acrylic glass panels, armport doors, the outer airlock door, and back panel. The manometer exhaust port on the back of the BACTRON900 will register as a leak under normal operating conditions. Some hydrogen gas naturally diffuses through the water-filled manometer. Do not seal or otherwise obstruct the manometer exhaust port. Doing so compromises the BACTRON900 overpressure and gas regulation system and voids the manufacturing defect warranty.
Fixing a Leak Contact your institutional maintenance department or Technical Support for assistance if a leak is confirmed, or if increased gas usage is not restricted to periods when users are working in the BACTRON900.
Excessive AMG Usage During Work Hours Check the following items if AMG usage is excessive when users are working in the BACTRON900.
· Verify users are operating the airlock correctly. · Confirm that users are employing correct sleeve donning, entry, and exit procedures. · Confirm the integrity of the sleeves and sleeve components. · Read the AMG Conservation Methods entry on page 75 for ways to reduce AMG usage.
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USER MAINTENANCE
LEAK CHECK UNIT EMPTY
Use this comprehensive procedure to check the atmospheric integrity of the workspace chamber to determine when the BACTRON900 can be taken out of operation. All samples should be removed from the chamber prior to carrying out this procedure since aerobic atmosphere will be present in the chamber. This procedure places the unit at a steady-state temperature and atmospheric pressure before performing a set of leak checks.
1. Turn off the BACTRON900. 2. Remove the left armport sleeve and open the left armport door. 3. Remove the O2 scrubber cartridge from the BACTRON900 to prevent its presence from
interfering with the leak check. · The catalytic production of water vapor reduces the volume and pressure of the
chamber atmosphere. This can interfere with a leak check. 4. Verify the manometer is filled with water up to the top reference ring (the fill line). 5. Check the integrity of the airlock door gaskets. 6. Replace the gasket if brittleness, dryness, or cracks are present. 7. Clean the gaskets with warm water and soap if sticky or dirty. Dry and seat securely on the
airlock mounting frames. 8. Close and latch both airlock doors. 9. Verify that the window panel of each airlock door sits flush against the door gasket. 10. Check the armport door ring seals for signs of damage or wear. 11. Close and secure the left armport. 12. Check that both the armport doors are correctly latched, with the locking bars in the
horizontal position, and the knobs snugly tightened clockwise using wrist strength. 13. Check that the AMG gas regulator is set to 50 psi. 14. Open the gas cylinder valve all the way On if not already opened. 15. Turn On the BACTRON900.
a. The AMG injecting light should illuminate. b. The manometer water level should be forced down to the bottom of the two measuring
rings (the red line).
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USER MAINTENANCE
16. Set the incubator(s) to Off to prevent heating. · An incubator actively heating from room temperature to achieve a setpoint increases air pressure in the chamber due to thermal expansion of the chamber atmosphere. This can interfere with performing an accurate leak check.
17. Monitor the BACTRON900 for 40 minutes. After the first 10 minutes, the unit should only inject once every 30 minutes. a. If there is a leak, the AMG Injecting light will illuminate more frequently than every 30 minutes. b. AMG chamber injections every 10 20 minutes are indicative of a large leak. c. Failure to obtain 0.5 inches of chamber overpressure as indicated by the manometer is indicative of a leak. Check to see if the chamber atmospheric pressure switch that sets the overpressure level needs to be adjusted. Adjusting the chamber pressure switch is a service-level procedure.
Locating Leaks See the Locating Leaks Entry (page 70) for instructions on using a hydrogen leak detector to pinpoint or find the leak. The hydrogen detector only finds leaks if AMG is present in the chamber.
DOOR GASKET MAINTENANCE AND USAGE
BACTRON900 door gaskets are subject to significant compression during airlock cycles. Users cycling the airlock more than 15 times per day will need to replace the door gaskets every 3 to 6 months. Heavy institutional users may wish to keep a pair of spare door gaskets on hand. Please see the parts list on page 88. Cleaning: Spilling sample media on door gaskets or the interior surfaces of airlock doors may cause the gaskets to stick to the doors. This can compromise the atmospheric integrity of the airlock. Gaskets can be cleaned with dish soap and warm water if permitted by your laboratory or production protocol.
SLEEVE MAINTENANCE AND USAGE
Sleeves may be washed with dish soap and warm water between uses. Disinfection should be carried out per laboratory or production protocols. Institutions with several users for each BACTRON900 may wish to keep a pair of sleeves in small, medium, and large sizes on hand or keep a pair of sleeves for each user.
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USER MAINTENANCE
CAUTION: The catalytic scrubber grows hot in the presence of free oxygen and AMG. DO NOT touch the scrubber cartridge body, and use caution when touching the scrubber cartridge handle.
O2 SCRUBBER CARTRIDGE: TEST IN THE CHAMBER
A test to see if a scrubber cartridge inside the workspace chambers is still active. 1. Place the scrubber in the anaerobic airlock. · If the airlock is not anaerobic, run a cycle before opening the inner airlock door. 2. Position the scrubber so the cartridge does not touch either airlock door.

Figure 12: Placement of scrubber cartridge in the Airlock

Auto Cycle

3. Close the inner airlock door.
4. Open the outer airlock door to allow aerobic atmosphere into the airlock chamber.
5. Close the outer airlock door.
6. Cycle the airlock with the scrubber cartridge inside. See page 60.
7. After the cycle is complete, open the inner door and place your hand near the scrubber cartridge to check for heat. Do not touch the cartridge!
· An activated scrubber will have grown warm in the presence of oxygen and AMG during an airlock cycle.
8. If the scrubber is cool or only slightly warm, reactivate by baking for a minimum of 8 hours. Please see the Reactivating Scrubber Cartridges procedure below.

REACTIVATING O2 SCRUBBER CARTRIDGES
Reactivate each cartridge after 24-hours of use in the workspace chamber. Failure to do so leaves the scrubber cartridge unable to remove free oxygen from the workspace chamber atmosphere.
1. Bake the catalyst cartridge at 200°C for a minimum of 8 hours.
2. Remove the cartridge handle before heating and reinstall the handle after baking.
3. Use appropriate Personal Protective Equipment (PPE) to prevent burns.
4. Reactivating helps remove buildups of volatiles that would otherwise prevent free oxygen and AMG hydrogen from impacting the palladium surfaces of the scrubber cartridge.

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USER MAINTENANCE
QUALITY CONTROL TEST SCRUBBER CARTRIDGES
Perform a quality control test on each O2 Scrubber cartridge once per month. 1. Place a reactivated O2 scrubber cartridge in the airlock with an aerobic atmosphere and run the auto cycle. · The palladium-coated pellets inside the catalyst cartridge should grow warm in the presence of oxygen and hydrogen indicating that the cartridge is ready for use. 2. If the cartridge is not hot after the cycle, bake the cartridge at 200°C for at least 8 hours. 3. Cleaning - While the scrubber is still hot from the oven, flow AMG over the cartridge in the aerobic air of the room. Utilize appropriate PPE.
A M G
· This helps remove buildups of hydrogen sulfides and other contaminants that interfere with cartridge effectiveness.
4. Allow the cartridge to cool after flowing AMG over it. 5. Retest by placing the cartridge in the BACTRON900 airlock with an aerobic atmosphere and
cycling the airlock.
AMG CONSERVATION METHODS
· Minimize the number of airlock cycles per day. · Use the dual AMG - N2 gas configuration for auto cycling the airlock. · Move a large number of items through the airlock in one transport to reduce the volume of AMG
used cycling the airlock. A greater volume of solids reduces the gas backfill volume. · When transporting a small number of items, place a large solid object in the airlock. This reduces
the volume of gas utilized. · Introduce small individual items, such as sealed microplates or transport tubes, into the
workspace chamber through the sleeve assemblies rather than the airlock. · Employ proper sleeve techniques when entering and exiting the workspace chamber. · Avoid fast or large movements while working in the chamber. Use a swimming motion,
withdrawing one arm partly from the armport while reaching in with the other arm.
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USER MAINTENANCE

Figure 13: Reassembling the Sleeve Assembly

Cuff-Ring and Sleeve Cuff

REPLACING SLEEVE COMPONENTS

Sleeve cuff attached to Cuff-Ring
1ST O-Ring added.

Replacing the sleeve components requires performing the following steps to disassemble the sleeve assembly:
1. Roll the black O-ring off the cuff on the outside of the sleeve assembly.
2. Pull the sleeve cuff and cuff-ring out of the sleeve body.
3. Remove the 2nd black O-ring.
4. Remove the sleeve cuff from the cuff-ring.
5. Inspect all components for brittleness, dryness, holes, or cracks. Replace as needed.
· The cuffs have the fastest rate of wear.

Sleeve Body attached.
2nd O-Ring added.

Reassemble the sleeve assembly: 1. Pull the wide, fringed side of the sleeve cuff on over the
cuff-ring. 2. Roll an O-ring onto the cuff-ring and over the sleeve cuff.
Place the ring into the groove opposite the fringed side of the cuff. 3. Pull the narrow end of the sleeve body over the sleeve cuff and cuff-ring. The fringed end of the sleeve cuff should be inside the sleeve body, and the body of the cuff should protrude from the sleeve. 4. Roll on the second O-ring. Place the O-ring into the unoccupied cuff-ring groove.
End of Procedure
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USER MAINTENANCE
Warning: Prior to any maintenance or cleaning of this unit, disconnect the power cord from the power supply. Avertissement: Avant tout entretien ou nettoyage de cet appareil, débranchez le cordon d'alimentation de l'alimentation.
CLEANING AND DISINFECTING
If a hazardous material or substance has spilled in the unit, immediately initiate your site Hazardous Material Spill Containment protocol. Contact your local Site Safety Officer and follow instructions per the site policy and procedures.
1. The BACTRON900 should be cleaned and disinfected prior to first use.
2. Periodic cleaning and disinfection are required to prevent microbiological contamination.
3. Do not use spray-on cleaners or disinfectants. These can leak through openings and coat electrical components.
4. Do not use cleaners or disinfectants that contain solvents capable of harming paint coatings, acrylic glass, or stainless steel surfaces. Do not use chlorine-based bleaches or abrasives--these will damage the chamber liner.
5. Consult the manufacturer or their agent if you have any doubts about the compatibility of decontamination or cleaning agents with any part of the equipment or material contained.
Warning: Never clean the unit with alcohol or flammable cleaners. Avertissement: Ne jamais nettoyer l'appareil avec de l'alcool ou des nettoyants inflammables
Cleaning Keep the following in mind when cleaning the BACTRON900 interior.
· Remove and clean the sleeve assemblies and all removable workspace chamber accessory items, except the currently installed O2 scrubber cartridge.
o Do not clean the catalytic O2 scrubbers using water, cleaning agents, or disinfectants! Clean the scrubber cartridge using heat and AMG (see page 74).
· Wash the armport doors, sample dish racks, shelf spacers, airlock gaskets, and sleeve assemblies with a mild soap and water solution.
· Clean the workspace chamber, incubator, and airlock interiors with a mild soap and water solution, including all corners.
o Take special care when cleaning around and chamber power outlets to prevent damage. Do not clean the airlock door alarm sensors.
o Do not use chloride-based cleaners, except Zephiran benzalkonium chloride solution. Other types may have adverse effects on microbiological samples.
· Rinse with distilled water and wipe dry with a soft cloth. Do not use deionized water. Please see page 65 for more information on DI water.
· Wipe down the interior surfaces with Zephiran. Allow the Zephiran to evaporate, do not wipe it up.
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USER MAINTENANCE

Disinfecting
Keep the following points in mind when carrying out your laboratory, clinical, or production space disinfection protocol:

· Turn off the BACTRON900 to safeguard against electrical shocks.

· Disinfect the BACTRON900 using commercially available disinfectants that are non-corrosive,

non-abrasive, and suitable for use on stainless steel, painted surfaces, and acrylic glass.

Contact your local Site Safety Officer for detailed information on the disinfectants

compatible with your cultivation or culturing applications.

Shock or

· Do not use overtly volatile disinfecting agents. Chlorines, amphyls, and quaternary

Fire Hazard

ammonias will evaporate into the chamber environment. Concentration in the chamber

atmosphere will increase over time, potentially leading to inhibited growth or metabolic

symptoms in sample populations.

· Open all the BACTRON900 doors to facilitate disinfection, ventilation of disinfectants, and drying.

· If possible, remove all interior accessories (shelf spacers, Petri dish racks, and other non-attached items) from the chamber when disinfecting.

· Disinfect all corners of the workspace chamber, the incubator interior(s), and the airlock interior.

· Take special care not to damage the armport door gaskets or the airlock door gaskets.

· The manometer glass water bottle can be autoclaved.

· After completion of your institutional protocol, allow all disinfectants to evaporate completely. Wipe down all surfaces except the door sensors with distilled water and Zephiran until the BACTRON900 no longer has a volatile odor. Use nonabrasive wipes.

Airlock Door Sensor; 1 per Door

Figure 14: Airlock Door Sensor

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USER MAINTENANCE
MAINTAINING THE ACRYLIC GLASS PANELS
Cleaning and Scratches The manufacturer recommends using Novus brand acrylic glass cleaner and scratch remover for cleaning and maintaining acrylic glass surfaces on the BACTRON900. Please see the Accessories section on page 91. Alcohol or alcohol-based solvents and other aggressive solvents should never be used to clean the BACTRON900 and may damage the acrylic glass panels.
Ultraviolet Lighting Never expose the BACTRON900 to sustained UV light. Prolonged exposure to UV will result in rapid aging of the acrylic glass, leaving it vulnerable to compression forces, and generating cracks across all exposed areas. UV light will also quickly age sleeve assemblies, turning the sleeves yellow and result in a rapid loss of elasticity. The BACTRON900 should not be exposed to direct sunlight. Damage from exposure to UV light is not covered under the manufacturing defect warranty. Disable or redirect laboratory disinfection UV lighting away from the BACTRON900. Verify that your laboratory or workspace environment does not use UV disinfection lighting at night. This type of light is usually referred to as short-wave UVC or germicidal UV light and operates at roughly 254 nm. Periodic use of long-wave (365 nm) UV hand lamps used for bacterial identification should not damage the acrylic glass.
ELECTRICAL COMPONENTS
Electrical components do not require maintenance. If the electrical systems fail to operate as specified, please contact your BACTRON900 distributor or technical support for assistance.
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USER MAINTENANCE

CALIBRATE THE TEMPERATURE DISPLAY
Note: Performing a temperature display calibration requires a temperature reference device. Please see the Temperature Reference Device entry on page 12 for the device requirements.
Temperature calibrations are performed to match an incubator temperature display to the actual air temperature inside the incubator which is supplied by a calibrated reference device.
Calibrate as often as required by your laboratory or production protocol, or regulatory compliance schedule. Always calibrate to the industry standards and use the calibration setup required by your laboratory protocol.
BACTRON900: Each temperature display must be separately calibrated to its incubator.
Suggested Calibration Setup

Temperature Reference Device

1. Use the airlock to introduce the Temperature Reference Device into the
workspace chamber. Cycle the airlock.
2. Introduce the sensor probes into the incubator through an open incubator door.

Painters tape or other non-stick tape recommended
3. Position the sensor head at least 2 inches (50 mm) from the shelf surface and as near as possible to the geometric center of the chamber. This ensures the air temperature is being measured. Secure the thermocouple probe to the shelving with non-marking tape.
4. Close the incubator doors. Seal any gaps along the side of the doors using painter's tape or any other non-stick tape. Do not seal the finger holes on the workspace incubator door!
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USER MAINTENANCE

Temperature Stabilization
The incubator air temperature must stabilize in order to perform an accurate calibration.
1. Allow the incubator to operate undisturbed with the doors shut for at least 24-hours when first putting the BACTRON900 into operation in a new environment.
2. Operating 8-hours undisturbed with the doors shut will suffice for a BACTRON900 that has been in operation for at least 1 day.
3. To be considered stabilized, the incubator chamber must operate at your calibration temperature for at least 1 hour with no fluctuations of ±0.2°C or greater. Begin Calibraiton
Fluctuations (Exaggerated) Required temperature stabilization period operating undisturbed with the incubator doors closed.
Start

Suggested Temperature Calibration
1 When the incubator temperature has stabilized, compare the reference device and incubator temperature display readings.
· If the readings are the same, or the difference between the two readings falls within the acceptable range of your protocol, the display is accurately showing the incubator air temperature. The Temperature Calibration procedure is now complete.
- Or -
· If a difference falls outside of your protocol range, advance to step 2.
2 A display calibration adjustment must be entered to match the display to the reference device. See next step.

Reference Device

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USER MAINTENANCE

Temperature Calibration (continued)

Place the display in its temperature calibration mode.

a. Press and hold both the UP and DOWN temperature arrow buttons simultaneously for approximately 5 seconds.

b. Release the buttons when the temperature display shows the letters "C O". The display will begin flashing the current temperature display value.

Note: If an arrow key is not pressed for five seconds, the display will stop flashing and store the last displayed number as the new current chamber temperature value.

Reference Device

Use the Up or Down arrows to adjust the current display temperature value until it matches the reference device temperature reading.

After matching the display to the reference device, wait

5 seconds.

· The temperature display will stop flashing and store the corrected chamber display value.

· The incubator will now begin heating or passively cooling in order to reach the setpoint with the corrected display value.

Cooling to SetPoint

After the incubator has achieved the corrected temperature, allow the BACTRON900 to sit at least one (1) hour undisturbed to stabilize.

· Failure to wait until the incubator is fully stabilized will result in an inaccurate reading.

SetPoint Achieved

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USER MAINTENANCE
Temperature Calibration (continued)
7 Compare the reference device reading with the chamber temperature display again.
· If the reference device and the chamber temperature display readings are the same or the difference falls within the range of your protocol, the incubator is now calibrated for temperature.
- OR · See the next step if the readings fail to match or fall
outside of your protocol range.
8 If the two readings are not the same and the difference still falls outside the acceptable range of your protocol, repeat steps 3 7 up to two more times.
Three calibration attempts may be required to successfully calibrate units that are more than ±2°C out of calibration.

Reference Device

Reference Device
X

9 If the temperature readings of the incubator temperature display and the reference device still fall outside your protocol after three calibration attempts, contact your BACTRON900 distributor or Technical Support for assistance.

End of Procedure

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UNIT SPECIFICATIONS

The BACTRON900s are either 110 120 volt units or 220 240 volt units. Please refer to the unit's data plate for individual electrical specifications.
Technical data specified applies to units with standard equipment at an ambient temperature of 25°C (77°F) and nominal voltage. The temperatures specified are determined in accordance with factory standards following DIN 12880 respecting the recommended wall clearances of 10% of the height, width, and depth of the inner chamber. All indications are average values, typical for units produced in the series. We reserve the right to alter technical specifications at any time.

POWER

Model
BACTRON900 BACTRON900-2

AC Voltage
110 120 220 240

Amperage
14.0 10.0

Frequency
50/60 Hz 50/60 Hz

UNIT WEIGHT
Model
BACTRON900

Unit Net Weight
473 lbs / 215 kgs

SHIPPING WEIGHT
Model
BACTRON900

Shipping Weight
795 lbs / 361 kgs

UNIT DIMENSIONS

Inches

Model
BACTRON900

Exterior W × D × H
88.5 x 32 x 33.6 in

Interior Dimensions W × D × H
42.5 x 28.9 x 25.0 in

Millimeters
Model
BACTRON900

Exterior W × D × H
2248 x 813 x 854 mm

Interior Dimensions W × D × H
1059 x 734 x 635 mm

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UNIT SPECIFICATIONS

SHELF DIMENSIONS
Model
BACTRON900

Inches W × D
27.3 x 7.4

Millimeters W × D
694 x 188

AIRLOCK DIMENSIONS
Interior

Model
BACTRON900

Inches W × D × H
16.0 x 10.0 x 11.5

Millimeters W × D × H
406 x 254 x 292

VOLUMES AND CAPACITY

Workspace Chamber Volume and Capacity

Model
BACTRON900

Cubic Feet
16.0

Plate Capacity
Model
BACTRON900

Plates
900

Airlock Plate Capacity
Model
BACTRON900

Plates
216

Liters
453.0

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UNIT SPECIFICATIONS

TEMPERATURE
Operating Temperature Range

Model
BACTRON900

Range
Ambient +5C to 70C

Temperature Uniformity
Model
BACTRON900

Workspace Incubator
±1.0C @ 37C

OXYGEN
When sitting undisturbed in a steady state, a BACTRON900 typically rests at lower than 4.7 parts per million oxygen in the workspace chamber.

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PARTS LIST

Description

Parts Number

Description

Parts Number

Anaerobic Monitoring Strips (Box of 100 packets)
Airlock Door Gasket, 1 Each 12in x 12in (burgundy)

9900706 3450507

Power Cords (2), 5-15P NEMA, 7.5 feet (2.29m), detachable

1800510

Foot Pedal Control

9830516

Airlock Door Gasket, 1 Each 9 x 9 (burgundy)

3450506

Fuse, Power Cord Inlet, Type T 12.5 Amp, 250V, 5x20mm

3300520

Armport Door Left

9900699

Armport Door Right Armport Door O-Ring

9900698 6000509

Armport Door Stand
O2 Scrubber Holder Assembly (includes catalyst cartridge. The unit requires 2 scrubbers for continual operation.)

9990761 9990759

Fuses, Vacuum Pump, Type T 10Amp, 250V, 5x20mm

3300516

Fuse, Secondary Inlet for Interior Outlet: Type T 16.0 Amp, 250V, 5x20mm

3300513

Gas Tubing white, 3/16ID, 5/16OD, 1 foot in length. Order by feet for an unbroken length.

8500527

Petri Dish Rack, 2 stacks of 11 Petri plates (for workspace incubators)

5110729

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PARTS

Description

Parts Number Description

Parts Number

Leveling Foot

2700506

Sleeve Assembly Size 6, Extra Small (2 cuffs, 2 cuff-rings, 4 0rings, 2 sleeve bodies, 2 selfgripping straps)
Sleeve Assembly Size 7, Small (2 cuffs, 2 cuff-rings, 4 O-rings, 2 sleeve bodies, 2 self-gripping straps)

9990812XS 9990738S

Sleeve Assembly Size 8, Medium (2 cuffs, 2 cuff-rings, 4 O-rings, 2 sleeve bodies, 2 selfgripping straps)
Sleeve Assembly Size 9, Large (2 cuffs, 2 cuff-rings, 4 O-rings, 2 sleeve bodies, 2 self-gripping straps)

9990738M 9990738L

Sleeve Cuff-Ring 4 Inches, interior diameter (for Small, Medium, and Large)
Sleeve Cuff O-Ring, Black, 4 Inches (For the 4-inch Sleeve Cuff Ring. Two O-rings are required).
Sleeve Cuff-Ring 3.5 Inches, interior diameter (for extra-small sleeve assembly)
Sleeve Cuff O-Ring, 3.5 inches, (For extra-small sleeve assembly. Only one is required.)

6400590 6000504 6400619 6000503

Sleeve Cuffs Latex, Size 7 (for Small sleeve assembly)
Sleeve Cuffs Latex, Size 8 (for Medium sleeve assembly)

3600500 3600501

Sleeve Cuffs Latex, Size 9 (for Large sleeve assembly)

3600502

Sleeve Cuffs Nitrile, Size 6 (for Extra Small sleeve assembly)

9990777

Sleeve Cuffs Nitrile, Size 7 (for Small sleeve assembly)
Sleeve Cuffs Nitrile, Size 8 (for Medium sleeve assembly)
Sleeve Cuffs Nitrile, Size 9 (for Large sleeve assembly)
Sleeve, Extra Small (10in to 3.5in dia.) (for XS sleeve assembly)
Sleeve, Standard (10in to 4.0in dia.)
(for S, M, L sleeve assemblies)

3600525 3600526 3600527 9990775 3600521

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PARTS ORDERING PARTS AND CONSUMABLES
Accessories and replacement parts can be ordered online at parts.sheldonmfg.com. If the required item is not listed online or if you require assistance in determining which part or accessory you need, contact the BACTRON900 manufacturer by emailing parts@sheldonmfg.com or by calling 1-800-322-4897 ext. 4 or (503) 640-3000 ext. 4. Please have the model, serial, and part numbers, and Part ID of the BACTRON900 unit ready. Tech Support needs this information to match your unit to its correct part.
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ACCESSORIES
Activated Carbon Media (2 lbs / 0.9 kgs) For scrubbing hydrogen sulfides, fatty acids, and some toxic or corrosive compounds from the chamber atmosphere. Part Number 1060500 Activated Carbon, Volatile Compounds Scrubber Fan Holds activated carbon scrubber media. Speeds the removal of sulfides, fatty acids, and toxic or corrosive compounds. For 110 120 volt units. Part Number 9490578 Acrylic Glass Cleaner (2 oz. / 59.2 ml) Novus brand acrylic glass cleaner. Part Number 1060503 Acrylic Glass Scratch Remover (2 oz. / 59.2 ml) Helps remove visible scratches and nicks from acrylic glass. Part Number 1060504
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ACCESSORIES

AMG Regulator The AMG Regulator is included with the BACTRON900. Part Number: 7150511
Anaerobic Indicator Strips 1 box of 100 Oxoid brand oxygen-detecting anaerobic indicator strips. Part Number 9900706
BACTRON900 LED Light Unit 220V A BACTRON900 LED lamp that sits on top of the workspace chamber. 220V. Part Number: 9730520
BACTRON900 Rolling Stand A rolling stand with cabinet. 29.3 inches high by 61.5 inches wide (74cm high by 156cm wide)
Part Number: BACSTAND-MD22
Gas Leak Detector A handheld gas detector for locating AMG leaks. Recommended for units that have been in service for 4 or more years. Part Number 4600501

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ACCESSORIES
Leica S6 Spotting Stereo Microscope and Assembly Requires the appropriate microscope adaptor. Part Number 9990516
Lukas Fiber Optic Micro Lite Illumination System A fiber optic, adjustable brightness, halogen light box and guide. Provides a stable, long-lasting light for use with chambers and stereo microscopes. Part Number 4650503
Nitrogen Regulator Kit Delivery gauge range of 2 60 PSIG. Includes barbed adaptor fitting and 16 feet (4.9 meters) of flexible tubing. Part Number 9740567 UV Viewing Lamp A handheld UV viewing lamp. Part Number 9490507
Zephiran Benzalkonium Chloride Chamber Cleaner 1 Gallon, 0.133%. Part Number 1060501
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APPENDICES

AMG USAGE

1. All values are approximate and affected by ambient temperatures. 2. All values are for automated cycles using the factory default settings. 3. Gas usage during manual cycles is dependent on the unit operator. Automated Commissioning Cycles

Model
BACTRON900 BACTRON900-2

Standard Cubic Feet
16.00 scf 16.00 scf

Standard Liters
453 sl 453 sl

Displayed supply gauge pressure: The BACTRON900 uses approximately 640 psi of gauge pressure from a size 300 cu.ft. compressed gas cylinder.

Airlock Auto Cycles
These usage figures are for BACTRON900s using a supplementary nitrogen supply (the dual gas configuration), so that AMG is only used during the final backfill of the airlock. Without the nitrogen supply, a 3-phase cycle uses approximately three times as much AMG. A 4-phase cycle consumes around four times as much, etc.

Model
BACTRON900

Standard Cubic Feet
1.2 scf

Standard Liters
33 sl

Sleeve Auto Cycles

Model
BACTRON900

Standard Cubic Feet
0.8 scf

Standard Liters
22 sl

Resting State
When a BACTRON900 is sitting sealed and undisturbed, it uses approximately 4 15 standard liters per day (0.15 0.53 scf per day).

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APPENDICES
PLC INPUTS AND OUTPUTS
The BACTRON900's Programmable Logic Controller (PLC) receives inputs from sensors and other components, triggering logic routines. These, in turn, generate outputs to power or depower the BACTRON900 components. These lamps are useful diagnostic indicators.

Outputs

Inputs
In 11 From outer airlock door sensor to PLC; registers door closed. 12 From inner airlock door sensor to PLC; registers door closed.

Shadow Box

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P.O. Box 627 Cornelius, Oregon, 97113
USA
support@sheldonmfg.com sheldonmanufacturing.com
800-322-4897 503-640-3000

References

Microsoft Word for Microsoft 365