New Features
- Expandable electropherogram image
- Adjustable gel/electropherogram sizes during analysis
- Inclusion Regions - analyze only the portion(s) of the sample you want
- New DQN (DNA Quality Number) in DNA and NGS analysis modes; a purity-based metric for rapid assessments of sample quality
- Molarity calculation options; settings to use Peak Maxima or Average Size of a Smear
- Concentration/Molarity units; view data in ng/µL / nmole/L or in pg/µL / pmole/L
- % Concentration based on either mass concentration or molarity
- Image resolution; easily change the exported image resolution to be higher for publication work
- Y-Axis; set the Y-axis to units of molarity with a simple right-click function
- X-Axis; set a start time for the data to display
- Add annotations to the gel image and/or the electropherogram
- Logo function; add your company logo to reports
- Recent file viewer; easily load the most recent runs you've looked at.
Main Screen
Once data is opened, the main screen appears similar to previous ProSize versions. Notable feature changes are below.
A – Y-Axis Molarity: Controls the display of the Y-axis, allowing it to show Molarity.
B – X-Axis Electropherogram Start Time: Allows adjustment of the starting point for the X-axis display.
C – Expandable Electropherogram Image: Enables expanding the electropherogram to fill the screen.
D – Gel Image Annotation: Provides tools for adding annotations to gel images.
E – Option Menu: Access to various software settings and configurations.
A – Y-Axis Molarity
Right-clicking on the Y-Axis opens an option to show the Y-Axis in either RFU (Relative Fluorescence Units), a traditional scale based upon sample mass, or in Molarity (nmole/L), which is based upon the relative number of molecules in the sample.
B – X-Axis Electropherogram Start Time
Right-clicking on the X-Axis and selecting "Set Display Starting Time (min)" opens a popup window. In this window, you can select a new Starting Time to show for the electropherogram view.
The image below shows the "Original Start Time" and the "Personalized Start Time" after using the "Set Display Starting Time" option.
C – Expandable Electropherogram Image
Extend Electropherogram: Selection of the blue arrow ➡️ indicated on the left will extend the electropherogram encompassing the entire bottom portion of the screen.
Minimize Electropherogram: To minimize the electropherogram to its original size and view the Peak Table, select the blue arrow ➡️ again.
D – Gel Image Annotation
ProSize data analysis software contains Annotation options for the Gel Image as well as the Electropherogram view.
Adding an Annotation: Selecting the annotation icon ? will open the "Add Annotation" box. Hover over the annotation wording to select it and move it to the desired location. Hover over the arrow ⬆️ to move the annotation to the desired location.
Annotation Editor: Selecting the editor icon ✏️ will open the "Annotation Editor" screen, allowing you to change colors or edit your annotations.
E – Option Menu
Selection of the "Option" menu from the top toolbar opens a new window. An extended description of each option is provided.
- The DQN (DNA Quality Number): A purity-based QC metric available in DNA and NGS modes. This setting can be enabled or disabled and is adjustable by the user, useful for quickly determining sample quality for downstream applications.
- Molarity Calculation: Set to use Peak Maxima or Average Size of a Smear.
- Concentration Unit: Settable to the range of interest for your samples (ng/µL or pg/µL).
- % Concentration based on: Setting to base % Conc. value on Mass Concentration or Molarity.
- Image Resolution: Adjust the exported image resolution higher for publication work.
- Edit Configuration: Make changes to your Global Configuration if needed.
Adjustable Gel/Electropherogram Size
Hovering anywhere over the center bar will display a resize symbol ↔️. You can then left-click/hold the mouse key and drag the line up or down to increase or decrease the size of the electropherogram relative to the Gel Image size.
Examples of each option are shown on the next slide.
Inclusion Regions
Under the "Option" – "Edit Configuration" window or the "Individual Parameters" window, you can enter Inclusion Regions based on size. When entering a Start and End Size, the Peak Table will show only the region(s) of interest.
Inclusion Regions can be applied to All or just Selected sample wells.
Logo
Under the "Generate PDF" option, you can now enter a logo for your company that will be shown at the top of each page of the PDF Report.
Recent Files
Selection of "File" – "Recent Files" will show the most recent files you have opened, allowing for quick access to the last few runs you've viewed.
User Manual and Tutorial Videos
As in previous ProSize versions, a complete User Manual and numerous Tutorial Videos are available for viewing on demand, under the "Help" – "User Manuals" and "Help - Tutorial Videos" options on the main screen.
Users are encouraged to view this material to further learn about the various features and functions of the ProSize software.
