NANOCOLOR® Ammonium 100

Overview

The test is suitable for the photometric determination of NH₄⁺. The test is in accordance with APHA 4500-NH₃ F, ISO 7150-1, DIN 38406-E5 and EPA 350.1. The test is suitable for surface water, ground and drinking water.

• Measuring range:
  • 4–80 mg/L NH₄-N
  • 5–100 mg/L NH₄⁺ / NH₃
• Number of tests: 20
• Wavelength for photometric determination: 585 nm
• Shelf life: 12 months
• Reaction time: 15 minutes
• Storage temperature: 15–25 °C
• Storage conditions: upright

Method

Photometric determination of a blue indophenol dye based on a reaction with hypochlorite and salicylate at a pH of 12.6 in the presence of sodium nitroprusside.

Interferences

The method can be applied for analyzing seawater. Turbidities cause higher measurement values.

Reagents and accessories

Contents of reagents set:

• 20 test tubes R0
• 1 reagent R2

Required devices:

• MACHEREY-NAGEL photometer
• Digital piston pipette 100–1000 μL (REF 91677) with pipette tips (REF 91667)
• Tweezers for sampling NANOFIX capsules (REF 916114)

Standards

• NANOCONTROL Multistandard Sewage (REF 925013)

Sampling and preparation

See DIN EN ISO 5667-3-A 21. Adjust to pH 1–13 with NaOH or HCl prior to analysis.

Quality control

The measurement of a blank value and a standard is recommended before every measuring series as quality control measure.

Quality data:

The following data were determined during production according to ISO 8466-1 and DIN 38402-A51:

• Number of LOTs: 27
• Standard deviation of the method: ± 0.85 mg/L NH₄-N
• Coefficient of variation of the process: ± 2.01 %
• Confidence interval: ± 1.95 mg/L NH₄-N

Specified data for procedure:

• Sensitivity (absorbance of 0.010 A corresponds to): 0.5 mg/L NH₄-N
• Accuracy of a measurement value: ± 0.146 mg/L NH₄-N

LOT-specific certificates are available at www.mn-net.com.

Procedure

1. Open test tube
2. Pipette 500 μL of sample into test tube
3. Add 1 NANOFIX R2
4. Seal test tube and shake vigorously
5. Wait 15 min
6. Clean outside of test tube
7. Measure

Notes

When using other photometers, make sure measurements are possible in test tubes (16 mm OD) and calibrate the method. Correction value e. g. for colored or turbid samples possible (see photometer manual). Information regarding safety can be found on the box' label and in the safety data sheet. You can download the SDS from www.mn-net.com/SDS. 08/2020